FastQCFastQC Report
Fri 27 May 2016
ERR765452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765452_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11341434
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1105420.9746739257134504No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT884000.7794428817378826No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG551780.48651696073001No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT488070.43034240643643473No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG435520.38400787766344185No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA433420.38215625995795593No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC414350.36534180774671No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG400910.35349145443160007No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC309370.27277855692675196No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT272390.2401724508558618No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC264780.23346254098026756No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC259110.22846317317545559No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA174610.15395760359756977No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG171660.1513565215827205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164950.14544016215233452No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG161460.14236294987036033No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC160670.14166638892401084No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC152060.13407475633151858No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT127780.11266652876523375No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG116110.1023768246590334No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC81400.024.9230251
GTACTAG25550.021.3705161
TCTACAC117950.020.5913263
TACACTC107750.019.8750155
ATCTACA109050.019.2071552
CTACACT134950.018.8682674
AGTACTC51950.018.3917835
GTACATA31900.017.7067341
CATGGGG214050.016.6011944
GTATAAG40950.016.0924451
CGTTAAC8750.015.6004751
TAGGCAT46800.015.5883175
GTATTAG34700.015.4640511
CTAGGCA51500.015.2621914
TAGTACT36250.014.93129354
CACTCTT140600.014.7642297
GTATAAA75950.014.7501731
TACTAGG36750.014.7288622
ATTATAC33050.014.6689263
GTATTAT31250.014.6106521
Produced by FastQC (version 0.11.5)