##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765452_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11341434 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.560077147210837 33.0 33.0 33.0 27.0 33.0 2 31.521745310160956 33.0 33.0 33.0 27.0 33.0 3 31.575306350149372 33.0 33.0 33.0 27.0 33.0 4 35.31372487817678 37.0 37.0 37.0 33.0 37.0 5 35.268855331697914 37.0 37.0 37.0 33.0 37.0 6 35.20232706022889 37.0 37.0 37.0 33.0 37.0 7 35.15506681077543 37.0 37.0 37.0 33.0 37.0 8 35.13587496960261 37.0 37.0 37.0 33.0 37.0 9 34.991194058881796 37.0 37.0 37.0 33.0 37.0 10-11 35.06317133265511 37.0 37.0 37.0 33.0 37.0 12-13 35.0451153266862 37.0 37.0 37.0 33.0 37.0 14-15 36.419982605374244 37.0 37.0 40.0 33.0 40.0 16-17 36.55969439137943 37.0 37.0 40.0 33.0 40.0 18-19 36.62637211484897 38.5 37.0 40.0 33.0 40.0 20-21 36.628967289321615 37.0 37.0 40.0 33.0 40.0 22-23 36.56936349495135 37.0 37.0 40.0 33.0 40.0 24-25 36.51755584875775 37.0 37.0 40.0 33.0 40.0 26-27 36.48823596733887 37.0 37.0 40.0 33.0 40.0 28-29 36.39848210552563 37.0 37.0 40.0 33.0 40.0 30-31 36.31845241086798 37.0 37.0 40.0 33.0 40.0 32-33 36.15702348574263 37.0 37.0 40.0 33.0 40.0 34-35 36.08794183345775 37.0 37.0 40.0 33.0 40.0 36-37 35.99802780671298 37.0 37.0 40.0 33.0 40.0 38-39 35.877552741566895 37.0 37.0 40.0 33.0 40.0 40-41 35.76136769829988 37.0 37.0 40.0 33.0 40.0 42-43 35.6067353563932 37.0 37.0 40.0 27.0 40.0 44-45 35.36453295941236 37.0 37.0 40.0 27.0 40.0 46-47 35.12725873112694 37.0 37.0 40.0 27.0 40.0 48-49 34.87462079310253 37.0 33.0 40.0 27.0 40.0 50-51 34.69574103239502 37.0 33.0 40.0 27.0 40.0 52-53 34.495267573747725 37.0 33.0 40.0 27.0 40.0 54-55 34.29456746827606 37.0 33.0 40.0 27.0 40.0 56-57 34.11180535018764 37.0 33.0 40.0 27.0 40.0 58-59 33.885246036788644 37.0 33.0 40.0 27.0 40.0 60-61 33.6292882804767 37.0 33.0 37.0 27.0 40.0 62-63 33.339211910945295 37.0 33.0 37.0 27.0 40.0 64-65 33.09009297237016 37.0 33.0 37.0 22.0 40.0 66-67 32.85614940756169 37.0 33.0 37.0 22.0 40.0 68-69 32.57878223335779 37.0 33.0 37.0 22.0 40.0 70-71 32.27451246464953 37.0 33.0 37.0 22.0 37.0 72-73 31.959752399916976 37.0 33.0 37.0 22.0 37.0 74-75 31.662085984893974 35.0 33.0 37.0 22.0 37.0 76-77 31.3939907863503 33.0 33.0 37.0 22.0 37.0 78-79 31.191962938725386 33.0 33.0 37.0 18.5 37.0 80-81 30.948771513373003 33.0 33.0 37.0 15.0 37.0 82-83 30.752427338553485 33.0 33.0 37.0 15.0 37.0 84-85 30.519992930347257 33.0 33.0 37.0 15.0 37.0 86-87 30.283795020982353 33.0 33.0 37.0 15.0 37.0 88-89 30.06554612053467 33.0 30.0 37.0 10.5 37.0 90-91 29.849616768038327 33.0 27.0 37.0 6.0 37.0 92-93 29.615731970048934 33.0 27.0 37.0 2.0 37.0 94-95 29.321926706975503 33.0 27.0 37.0 2.0 37.0 96-97 29.045160206372493 33.0 27.0 37.0 2.0 37.0 98-99 28.683586264311902 33.0 27.0 37.0 2.0 37.0 100 28.341124764293475 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 113681.0 3 26334.0 4 15012.0 5 11585.0 6 14441.0 7 23067.0 8 27572.0 9 25732.0 10 25817.0 11 28686.0 12 32989.0 13 36310.0 14 38964.0 15 44719.0 16 51010.0 17 57333.0 18 53931.0 19 41881.0 20 36753.0 21 39289.0 22 46278.0 23 57633.0 24 72413.0 25 88685.0 26 107124.0 27 129516.0 28 163988.0 29 211472.0 30 274493.0 31 360908.0 32 486401.0 33 670333.0 34 935189.0 35 1361800.0 36 2128465.0 37 2697196.0 38 803957.0 39 477.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.114352761247964 18.114401436556495 12.838042081567824 24.933203720627713 2 14.926382086954742 21.68817310918973 36.960546684371 26.424898119484524 3 18.37603320329609 26.69372387026339 29.361600136485738 25.568642789954783 4 13.136044679861195 18.021060832662762 35.634327601151504 33.20856688632453 5 13.846082439221705 37.600973610441464 33.14375154862087 15.40919240171596 6 31.590622500703518 35.6332291865181 17.807077086075054 14.969071226703326 7 26.80379742370337 32.344223227305726 21.978761673160673 18.873217675830233 8 27.34437070148547 33.00625959224593 20.462584916427797 19.186784789840797 9 27.57552703058751 15.31993416989486 19.071754602975403 38.032784196542224 10-11 24.639975258013433 26.315138972165176 26.889290457217907 22.15559531260348 12-13 26.412439880528854 23.846375164904348 26.55167970245641 23.189505252110383 14-15 24.734068432772986 23.871138072605298 24.74952648152016 26.645267013101552 16-17 23.715525398154426 26.678492410738947 26.225640540440093 23.38034165066654 18-19 24.655087462137832 26.114500346349782 27.209611100686914 22.020801090825472 20-21 24.74803720307727 25.68206898214971 26.646341896246582 22.923551918526442 22-23 23.86219908104502 26.203453157855055 25.212589356929925 24.721758404169996 24-25 23.882879360758082 26.58110958455518 24.99306084221801 24.54295021246872 26-27 24.454063745378228 25.581081722117325 25.1347625000507 24.83009203245374 28-29 24.073531895503194 25.166666762186757 26.723628089378764 24.03617325293128 30-31 25.188812102596547 25.15369749539608 26.245627316616222 23.41186308539114 32-33 23.116243501483147 25.841957022365953 26.516338233771847 24.525461242379052 34-35 23.097312914751345 26.08811196185597 26.710431855442618 24.104143267950068 36-37 24.38770077711527 25.96173481443966 25.778287962225583 23.87227644621949 38-39 24.84574701929227 25.102517900293737 25.748251940627615 24.30348313978638 40-41 23.98526941125787 25.899537924392984 25.8317687163722 24.28342394797695 42-43 25.231694686932887 25.353892638267787 25.505094858375056 23.909317816424274 44-45 25.020608153237017 25.109001237503183 26.02229798212476 23.848092627135046 46-47 24.22593731631817 25.279447686091682 26.995500021074086 23.49911497651606 48-49 24.74015499680428 25.76151622658203 26.41944208610157 23.078886690512128 50-51 23.753241138785857 26.25332336034991 25.78339075029007 24.210044750574163 52-53 23.412623255379238 26.64701281776588 24.682703643060005 25.257660283794873 54-55 23.989418677577184 26.106741294382307 24.655164622230256 25.24867540581025 56-57 24.478666997271453 25.039238743683 24.926530963513084 25.555563295532462 58-59 24.22999249461435 24.935702451979775 26.274881596831424 24.559423456574454 60-61 24.42146164159672 25.397714672189004 25.717578559521296 24.463245126692975 62-63 22.857000139228695 25.781900499010103 26.135165755772416 25.225933605988793 64-65 22.869045838248482 25.71387293417774 25.948547935752746 25.468533291821032 66-67 24.23585985952023 25.548388422220885 25.229517804741036 24.986233913517854 68-69 24.50581255656822 24.810581061677155 25.39383823594893 25.289768145805695 70-71 23.548582555445556 25.383801958771212 25.53843018972268 25.529185296060557 72-73 24.57874166613616 24.90739220577958 25.51105341872919 25.00281270935507 74-75 24.72009380472054 24.54257215368763 25.79358244264272 24.94375159894911 76-77 23.92329753010069 25.189345544840275 26.319401056339082 24.567955868719952 78-79 24.153689566487728 25.074570685391656 26.143488816061446 24.628250932059174 80-81 23.49075572880826 25.290193797880594 25.98462125078677 25.234429222524373 82-83 23.532431924607764 25.745278396477765 24.9223003830292 25.799989295885272 84-85 23.722429123867006 25.541013168913977 24.851455807154775 25.885101900064246 86-87 23.822590116581523 24.612971744278937 25.341606253890607 26.222831885248937 88-89 23.927081014672915 24.222785027097824 26.05296775969228 25.79716619853698 90-91 24.28544516922134 24.692019539658634 25.649463341846285 25.37307194927374 92-93 23.229903781507975 25.035694525575604 25.909354024534064 25.82504766838236 94-95 22.814719732068077 25.227426959229902 26.09562312409984 25.862230184602176 96-97 23.86123672128933 24.904192955456043 25.405479589386353 25.82909073386827 98-99 23.961931915647398 24.562848917505146 25.36088415410897 26.114335012738486 100 23.65622324521687 24.883387720948768 25.596772188339568 25.863616845494793 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1886.0 1 1684.5 2 1628.5 3 1720.0 4 1416.5 5 1381.0 6 1861.5 7 3232.5 8 5220.5 9 5118.0 10 3173.0 11 2177.5 12 2176.5 13 2317.0 14 2662.0 15 3128.0 16 3659.5 17 4298.5 18 5001.0 19 6015.5 20 7540.0 21 9541.0 22 12160.0 23 15322.0 24 19037.0 25 24678.0 26 32282.5 27 40097.5 28 47726.5 29 58216.0 30 69905.5 31 80215.0 32 91886.0 33 105205.0 34 120183.5 35 134136.0 36 149524.5 37 168210.5 38 184092.0 39 199383.0 40 214076.0 41 227251.0 42 241614.5 43 266534.0 44 298689.0 45 329153.0 46 373059.5 47 426129.5 48 536025.0 49 648558.0 50 764649.0 51 752467.0 52 587627.5 53 508781.0 54 472376.0 55 427427.5 56 388643.0 57 354769.0 58 331287.0 59 304492.0 60 261379.0 61 214754.0 62 172974.0 63 134869.0 64 100715.0 65 77705.5 66 60841.5 67 46026.0 68 35246.5 69 28836.0 70 24200.0 71 20496.0 72 17481.0 73 16538.5 74 13126.0 75 9608.5 76 7276.0 77 5210.0 78 3801.0 79 2501.5 80 1727.0 81 1231.5 82 896.0 83 674.5 84 500.5 85 341.0 86 235.5 87 167.5 88 113.5 89 60.5 90 34.5 91 24.0 92 17.0 93 12.5 94 7.0 95 6.5 96 7.0 97 2.5 98 2.0 99 9.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008658517079938921 2 0.04942937550930508 3 0.048062705298113094 4 0.046907648538976644 5 0.07732708227195961 6 0.017572733747778277 7 0.01539487863704008 8 0.019371448090250314 9 0.006401306924679895 10-11 0.017678540473806046 12-13 0.03351428046929515 14-15 0.009377121094210837 16-17 0.005638616774563075 18-19 0.00262753369635621 20-21 0.008116257609046617 22-23 0.012158956266024208 24-25 0.0 26-27 0.0 28-29 1.3666702111919885E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.7634454337961145E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 4.32044131280048E-4 46-47 0.004338075767138442 48-49 0.00432484992638497 50-51 0.0011197878504605326 52-53 0.0012696807123332022 54-55 0.006141198723194969 56-57 0.005307970755726304 58-59 0.013904767245482362 60-61 0.007309481323084894 62-63 0.0033593635513815977 64-65 0.0019177469092532744 66-67 0.0013843046655299498 68-69 3.438718595902423E-4 70-71 3.3505463242126173E-4 72-73 4.93764721462912E-4 74-75 4.408613584490286E-6 76-77 0.0 78-79 1.1903256678123771E-4 80-81 3.967752226041257E-5 82-83 8.817227168980572E-6 84-85 2.46882360731456E-4 86-87 1.23441180365728E-4 88-89 2.2483929280900456E-4 90-91 7.891418316237611E-4 92-93 5.202164029698537E-4 94-95 4.01183836188616E-4 96-97 1.6311870262614056E-4 98-99 1.5430147545716E-4 100 3.526890867592229E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1341434E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.42434022671508 #Duplication Level Percentage of deduplicated Percentage of total 1 72.59786232644689 27.16927099437169 2 13.753936294547527 10.294639826874231 3 5.054425298592361 5.674755960751095 4 2.509120497739128 3.7560871670885523 5 1.3702194018294322 2.563977853965535 6 0.9458945839872042 2.123968843784655 7 0.6286918854098933 1.646986531214837 8 0.45285382441830013 1.3558204478399645 9 0.36739563588120705 1.237458534752576 >10 2.012537482065383 13.900815475674294 >50 0.16417070138902626 4.26315259039118 >100 0.12038123626100805 8.90437595166803 >500 0.013671572963435581 3.51036244485924 >1k 0.007698709569226032 5.371054959362033 >5k 5.465130146204227E-4 1.4771432136399147 >10k+ 5.940358854569813E-4 6.750129203762157 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 110542 0.9746739257134504 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 88400 0.7794428817378826 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 55178 0.48651696073001 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 48807 0.43034240643643473 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 43552 0.38400787766344185 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 43342 0.38215625995795593 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 41435 0.36534180774671 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 40091 0.35349145443160007 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 30937 0.27277855692675196 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 27239 0.2401724508558618 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 26478 0.23346254098026756 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 25911 0.22846317317545559 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 17461 0.15395760359756977 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 17166 0.1513565215827205 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16495 0.14544016215233452 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 16146 0.14236294987036033 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 16067 0.14166638892401084 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 15206 0.13407475633151858 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 12778 0.11266652876523375 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 11611 0.1023768246590334 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.466680844767954E-4 0.0 0.0 5.290336301388343E-5 0.0 2 7.759159908702903E-4 0.0 0.0 7.053781735184458E-5 0.0 3 8.111848995462125E-4 0.0 0.0 7.935504452082514E-5 0.0 4 9.169916255739795E-4 0.0 0.0 7.935504452082514E-5 0.0 5 0.0038707627271824707 8.817227168980572E-6 0.0 3.70323541097184E-4 0.0 6 0.009328626344781444 8.817227168980572E-6 0.0 8.111848995462125E-4 0.0 7 0.0108011032820012 8.817227168980572E-6 0.0 0.0010227983516017463 0.0 8 0.012899603348218576 8.817227168980572E-6 0.0 0.001172691213474416 0.0 9 0.014918748369915127 8.817227168980572E-6 0.0 0.001296132393840144 0.0 10-11 0.030661907480129938 8.817227168980572E-6 0.0 0.0023497910405333227 0.0 12-13 0.052876911332376486 1.7634454337961145E-5 0.0 0.004003021134717179 1.7634454337961145E-5 14-15 0.07803246044547807 1.7634454337961145E-5 0.0 0.0057841010228512554 2.2043067922451428E-5 16-17 0.08866603641126863 1.7634454337961145E-5 0.0 0.006590877308812977 2.6451681506941715E-5 18-19 0.09348024244553202 2.2043067922451428E-5 0.0 0.006978835304248122 2.6451681506941715E-5 20-21 0.10971275766362526 2.6451681506941715E-5 0.0 0.008213247107905403 2.6451681506941715E-5 22-23 0.11520589018990016 2.6451681506941715E-5 0.0 0.009959058087363555 2.6451681506941715E-5 24-25 0.13808218607982026 2.6451681506941715E-5 0.0 0.013640250430412944 2.6451681506941715E-5 26-27 0.20012460505435203 2.6451681506941715E-5 0.0 0.02130242084025706 2.6451681506941715E-5 28-29 0.21423657713830546 2.6451681506941715E-5 0.0 0.03950558633061745 2.6451681506941715E-5 30-31 0.2202014313181208 2.6451681506941715E-5 0.0 0.07365911576966369 2.6451681506941715E-5 32-33 0.22592381175078918 2.6451681506941715E-5 0.0 0.11953074011628512 2.6451681506941715E-5 34-35 0.23001059654361167 3.526890867592229E-5 0.0 0.17960691743213425 2.6451681506941715E-5 36-37 0.24072793616750757 3.526890867592229E-5 0.0 0.27085640140391415 2.6451681506941715E-5 38-39 0.25232699850830154 3.526890867592229E-5 0.0 0.42390583060307896 3.9677522260412576E-5 40-41 0.2608179882720298 3.526890867592229E-5 0.0 0.6279585103612118 4.4086135844902856E-5 42-43 0.2797265319359087 3.526890867592229E-5 0.0 0.8067233825987083 4.849474942939314E-5 44-45 0.30032357460264725 3.526890867592229E-5 0.0 0.9962320461416079 5.290336301388343E-5 46-47 0.3069982155695655 3.526890867592229E-5 0.0 1.1847796319230883 5.290336301388343E-5 48-49 0.311534678948006 3.526890867592229E-5 0.0 1.3838285352628248 6.1720590182864E-5 50-51 0.3187824396809081 3.526890867592229E-5 0.0 1.623652705645512 6.1720590182864E-5 52-53 0.3212292202203002 4.4086135844902856E-5 0.0 1.9326700662367742 7.053781735184456E-5 54-55 0.329482144850466 4.4086135844902856E-5 0.0 2.1819418955310237 8.817227168980571E-5 56-57 0.34639358656057073 4.4086135844902856E-5 0.0 2.460808747817957 9.258088527429599E-5 58-59 0.35210274115248563 4.4086135844902856E-5 0.0 2.763508565142644 9.698949885878629E-5 60-61 0.35678909739279885 4.4086135844902856E-5 0.0 3.045990480568859 9.698949885878629E-5 62-63 0.3611536248414442 4.4086135844902856E-5 0.0 3.35199234946833 1.0580672602776686E-4 64-65 0.3643763213717066 4.4086135844902856E-5 0.0 3.652360010206822 1.0580672602776686E-4 66-67 0.36941536669877906 5.290336301388343E-5 0.0 4.00029220290838 1.1021533961225714E-4 68-69 0.3747850580446882 6.1720590182864E-5 0.0 4.402966150488554 1.1462395319674743E-4 70-71 0.3811158271520162 6.1720590182864E-5 0.0 4.8533104367578215 1.1903256678123773E-4 72-73 0.39746737493689066 6.1720590182864E-5 0.0 5.2691220528197755 1.3666702111919888E-4 74-75 0.41459483871263547 6.1720590182864E-5 0.0 5.677187734813781 1.4107563470368916E-4 76-77 0.4200482937166499 6.1720590182864E-5 0.0 6.06480626700292 1.4107563470368916E-4 78-79 0.42350023815330584 6.1720590182864E-5 0.0 6.445878008019092 1.4107563470368916E-4 80-81 0.4305848801835817 6.1720590182864E-5 0.0 6.925949575688577 1.4107563470368916E-4 82-83 0.4332873603108743 6.1720590182864E-5 0.0 7.451099217259475 1.4989286187266972E-4 84-85 0.4434359887823709 6.1720590182864E-5 0.0 7.9575827889136415 1.4989286187266972E-4 86-87 0.46200066058665945 6.1720590182864E-5 0.0 8.448138039687045 1.4989286187266972E-4 88 0.46646658614774816 6.1720590182864E-5 0.0 8.830232579054817 1.4989286187266972E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 8140 0.0 24.923025 1 GTACTAG 2555 0.0 21.370516 1 TCTACAC 11795 0.0 20.591326 3 TACACTC 10775 0.0 19.875015 5 ATCTACA 10905 0.0 19.207155 2 CTACACT 13495 0.0 18.868267 4 AGTACTC 5195 0.0 18.391783 5 GTACATA 3190 0.0 17.706734 1 CATGGGG 21405 0.0 16.601194 4 GTATAAG 4095 0.0 16.092445 1 CGTTAAC 875 0.0 15.600475 1 TAGGCAT 4680 0.0 15.588317 5 GTATTAG 3470 0.0 15.464051 1 CTAGGCA 5150 0.0 15.262191 4 TAGTACT 3625 0.0 14.9312935 4 CACTCTT 14060 0.0 14.764229 7 GTATAAA 7595 0.0 14.750173 1 TACTAGG 3675 0.0 14.728862 2 ATTATAC 3305 0.0 14.668926 3 GTATTAT 3125 0.0 14.610652 1 >>END_MODULE