##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765449_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6660199 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51685227423385 34.0 31.0 34.0 31.0 34.0 2 32.646775269027245 34.0 31.0 34.0 31.0 34.0 3 32.691335198843156 34.0 31.0 34.0 31.0 34.0 4 36.19333731619731 37.0 35.0 37.0 35.0 37.0 5 36.13892497806747 37.0 35.0 37.0 35.0 37.0 6 36.19939554358661 37.0 37.0 37.0 35.0 37.0 7 36.153997951112274 37.0 36.0 37.0 35.0 37.0 8 36.16783297315891 37.0 37.0 37.0 35.0 37.0 9 37.896333578020716 39.0 38.0 39.0 35.0 39.0 10-11 37.8558501029774 39.0 38.0 39.0 35.0 39.0 12-13 37.82159249295704 39.0 38.0 39.0 35.0 39.0 14-15 39.16934328839123 40.5 39.0 41.0 36.0 41.0 16-17 39.15088100220429 40.0 38.5 41.0 36.0 41.0 18-19 39.197711735039746 40.0 39.0 41.0 36.0 41.0 20-21 39.19330143138366 40.0 39.0 41.0 36.0 41.0 22-23 39.13097701735339 40.0 39.0 41.0 35.5 41.0 24-25 39.067660290630954 40.0 39.0 41.0 35.0 41.0 26-27 38.927215162790176 40.0 38.0 41.0 35.0 41.0 28-29 38.79495590146781 40.0 38.0 41.0 35.0 41.0 30-31 38.60314136259292 40.0 38.0 41.0 35.0 41.0 32-33 38.32993443589298 40.0 38.0 41.0 34.0 41.0 34-35 38.252791170353916 40.0 38.0 41.0 34.0 41.0 36-37 38.13642925684353 40.0 38.0 41.0 34.0 41.0 38-39 37.9608430618965 40.0 37.5 41.0 33.5 41.0 40-41 37.72907641348254 40.0 37.0 41.0 33.0 41.0 42-43 37.45528099085328 40.0 37.0 41.0 33.0 41.0 44-45 37.07465257419486 40.0 36.0 41.0 32.0 41.0 46-47 36.697000570103086 40.0 36.0 41.0 31.0 41.0 48-49 36.37127802037146 39.0 35.5 41.0 30.5 41.0 50-51 36.09313040045801 39.0 35.0 41.0 30.0 41.0 52-53 35.941469691821524 39.0 35.0 41.0 30.0 41.0 54-55 35.9137842577977 39.0 35.0 41.0 30.0 41.0 56-57 35.728352936601446 39.0 35.0 41.0 29.0 41.0 58-59 35.58640582661269 39.0 35.0 41.0 29.0 41.0 60-61 35.25193804269212 38.0 35.0 40.5 29.0 41.0 62-63 34.90667110697443 37.0 35.0 40.0 28.0 41.0 64-65 34.68236076729839 37.0 35.0 40.0 28.0 41.0 66-67 34.322177685681766 36.5 35.0 39.0 28.0 41.0 68-69 33.96783572082456 36.0 34.5 39.0 28.0 41.0 70-71 33.58032380113568 35.0 34.0 38.5 27.0 40.5 72-73 33.189458002681306 35.0 34.0 37.0 26.0 39.5 74-75 32.832555603819046 35.0 34.0 37.0 26.0 39.0 76-77 31.408152293947975 34.5 31.5 35.5 24.0 37.5 78-79 32.15553994707966 35.0 33.5 36.0 26.0 37.0 80-81 32.1037372907326 35.0 34.0 35.5 26.0 37.0 82-83 31.929010829856587 35.0 34.0 35.0 25.0 37.0 84-85 31.74118611170627 35.0 34.0 35.0 25.0 36.0 86-87 31.56210362483163 35.0 33.0 35.0 24.5 36.0 88-89 31.407931880113495 35.0 33.0 35.0 24.0 36.0 90-91 31.27847658906288 35.0 33.0 35.0 23.5 35.5 92-93 31.125058575577096 35.0 33.0 35.0 20.5 35.0 94-95 31.03053474828605 35.0 33.0 35.0 20.0 35.0 96-97 30.91682748518475 35.0 33.0 35.0 18.5 35.0 98-99 30.781336563667242 35.0 33.0 35.0 9.5 35.0 100 30.45875686297061 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4124832139988044 1101 2 0.39249222201483747 1101 3 0.41397985871319776 1101 4 0.24343074654109387 1101 5 0.29969306569969945 1101 6 0.2970064025234791 1101 7 0.3114866689112006 1101 8 0.32617654304478094 1101 9 0.3554736923943338 1101 10-11 0.4098763487141639 1101 12-13 0.42097463548953584 1101 14-15 0.5331641506214027 1101 16-17 0.5279011990192757 1101 18-19 0.5069481490380241 1101 20-21 0.5165546505851921 1101 22-23 0.4735520515593734 1101 24-25 0.5269037701989703 1101 26-27 0.5702815427246009 1101 28-29 0.5537258504543274 1101 30-31 0.6187440716448478 1101 32-33 0.6821751499588018 1101 34-35 0.6060213551043816 1101 36-37 0.7242034622659546 1101 38-39 0.7365001760464978 1101 40-41 0.8004037517557521 1101 42-43 0.8732719723857585 1101 44-45 0.906138603469671 1101 46-47 0.9002721368156443 1101 48-49 0.9724012860514151 1101 50-51 0.9783771623376225 1101 52-53 0.9163426726810542 1101 54-55 1.0607123149378737 1101 56-57 1.0716442556490762 1101 58-59 1.1085843322598308 1101 60-61 1.2038751044794154 1101 62-63 1.1957561317399055 1101 64-65 1.117988144979094 1101 66-67 1.1019816537475435 1101 68-69 1.167926106837875 1101 70-71 1.1317672856178262 1101 72-73 1.1216913825911945 1101 74-75 1.116671842132675 1101 76-77 1.1091940348353084 1101 78-79 0.9544899260098418 1101 80-81 1.033383564045856 1101 82-83 1.0527111170034793 1101 84-85 1.0515829191214863 1101 86-87 1.0323926024234247 1101 88-89 1.0865113077538062 1101 90-91 1.0717592100179907 1101 92-93 1.1021366943319855 1101 94-95 1.1203456983569247 1101 96-97 1.2250403420057232 1101 98-99 1.1912590652142718 1101 100 1.2459415524298834 1102 1 0.3831934778533679 1102 2 0.3486147702895366 1102 3 0.3732242994252317 1102 4 0.2701954422298911 1102 5 0.32270235939486724 1102 6 0.29376154058257953 1102 7 0.3368210526506985 1102 8 0.2910942434550776 1102 9 0.35378311165835186 1102 10-11 0.34594337075936465 1102 12-13 0.3613725834651049 1102 14-15 0.47978438880233654 1102 16-17 0.504743108892292 1102 18-19 0.4825250393025726 1102 20-21 0.5011536367033003 1102 22-23 0.5116082802813224 1102 24-25 0.5082915526955745 1102 26-27 0.5506174194271765 1102 28-29 0.6171501212351771 1102 30-31 0.6653798393077963 1102 32-33 0.6334570653771081 1102 34-35 0.691013776983759 1102 36-37 0.7234231557819584 1102 38-39 0.6809907757111162 1102 40-41 0.7784616933862267 1102 42-43 0.7709343521155816 1102 44-45 0.9202644335971257 1102 46-47 0.9656650860309028 1102 48-49 0.9989558671819623 1102 50-51 1.0324064297459685 1102 52-53 1.0707782060021245 1102 54-55 1.109837131983241 1102 56-57 1.1564308952291285 1102 58-59 1.10193798055505 1102 60-61 1.1587837871261186 1102 62-63 1.1205646125699857 1102 64-65 1.1642135144208083 1102 66-67 1.1676480351516147 1102 68-69 1.1392247247806253 1102 70-71 1.1615568977940072 1102 72-73 1.1725944253032736 1102 74-75 1.173373613426726 1102 76-77 1.2291689477235757 1102 78-79 1.201342539894931 1102 80-81 1.1921770166029546 1102 82-83 1.1967430637473448 1102 84-85 1.17298547795356 1102 86-87 1.1688784206071823 1102 88-89 1.1961541163372793 1102 90-91 1.2418893510763418 1102 92-93 1.2039354482410154 1102 94-95 1.238378926116436 1102 96-97 1.293172611869462 1102 98-99 1.2860687190867743 1102 100 1.334551243893067 1103 1 0.37698271606168987 1103 2 0.2585802857507886 1103 3 0.2771389140553495 1103 4 0.23855608442699605 1103 5 0.2702412233323628 1103 6 0.2400980229531271 1103 7 0.2453617578285403 1103 8 0.24705390824608742 1103 9 0.31283960504193686 1103 10-11 0.3124398001297308 1103 12-13 0.28486615633176626 1103 14-15 0.39013911149777414 1103 16-17 0.39354389357041697 1103 18-19 0.3776588475726612 1103 20-21 0.40873853164935525 1103 22-23 0.402969180596223 1103 24-25 0.4277831982826257 1103 26-27 0.45687792912379876 1103 28-29 0.46746037883674774 1103 30-31 0.5039712732382426 1103 32-33 0.5299252566596024 1103 34-35 0.5253869120826167 1103 36-37 0.6448270929388826 1103 38-39 0.6321898189544513 1103 40-41 0.5925596060735785 1103 42-43 0.6761113520983741 1103 44-45 0.6587294414267006 1103 46-47 0.6379778559791092 1103 48-49 0.5153164233397973 1103 50-51 0.6762888140661758 1103 52-53 0.6706782111644785 1103 54-55 0.6792617529802669 1103 56-57 0.6275714145621549 1103 58-59 0.7241444403833199 1103 60-61 0.7506035864825478 1103 62-63 0.7535510527812974 1103 64-65 0.7203458772311961 1103 66-67 0.7068941590825801 1103 68-69 0.7080348705311508 1103 70-71 0.5548378504207889 1103 72-73 0.6240632704842071 1103 74-75 0.6664044499004973 1103 76-77 0.6674939352478866 1103 78-79 0.7024925579631756 1103 80-81 0.6428040363747769 1103 82-83 0.6721436109570895 1103 84-85 0.6955174759574518 1103 86-87 0.662860094243026 1103 88-89 0.6800873498650581 1103 90-91 0.7432168853629939 1103 92-93 0.7504173885985956 1103 94-95 0.7906029895790034 1103 96-97 0.8194340679980421 1103 98-99 0.7662513116220104 1103 100 0.8203758958642275 1104 1 0.23958420607494446 1104 2 0.16373021643013175 1104 3 0.21445612843138662 1104 4 0.18259438599470457 1104 5 0.20848986358100774 1104 6 0.20370775526874496 1104 7 0.1844894754268509 1104 8 0.14439357946810816 1104 9 0.22598539171713128 1104 10-11 0.23000545669538752 1104 12-13 0.24779075678578266 1104 14-15 0.35107492513947136 1104 16-17 0.2684373225814909 1104 18-19 0.2656817767132367 1104 20-21 0.2982174993047906 1104 22-23 0.26176309256660346 1104 24-25 0.2945186980769421 1104 26-27 0.2977948966878259 1104 28-29 0.3029888155416529 1104 30-31 0.35804083766075223 1104 32-33 0.2873556084429012 1104 34-35 0.3550702614130259 1104 36-37 0.36361827778888767 1104 38-39 0.4076446691151858 1104 40-41 0.4177827576496682 1104 42-43 0.42107685935800276 1104 44-45 0.39091048260774386 1104 46-47 0.408292038528586 1104 48-49 0.43845445524252824 1104 50-51 0.39595435331994366 1104 52-53 0.39244701534504856 1104 54-55 0.4512992166059604 1104 56-57 0.36050617696749754 1104 58-59 0.4103166298496248 1104 60-61 0.4624494051518937 1104 62-63 0.4412423092372606 1104 64-65 0.4369998985910968 1104 66-67 0.41555253774096457 1104 68-69 0.43470220755143174 1104 70-71 0.4074322103092598 1104 72-73 0.42009994187382205 1104 74-75 0.49404360159847016 1104 76-77 0.41590382601071596 1104 78-79 0.47763586128295543 1104 80-81 0.43493024567744953 1104 82-83 0.41337876795695294 1104 84-85 0.38137152169385047 1104 86-87 0.4095960358613233 1104 88-89 0.42088616760505104 1104 90-91 0.4433006338585095 1104 92-93 0.43773483150427595 1104 94-95 0.47678070946260576 1104 96-97 0.5119875363162159 1104 98-99 0.48695154167517885 1104 100 0.5731788576083652 1105 1 0.18883979577289978 1105 2 0.11666574324305401 1105 3 0.17507467359564544 1105 4 0.14846355459054195 1105 5 0.18359410383921926 1105 6 0.16388644198952562 1105 7 0.13733553121884512 1105 8 0.1351906402337093 1105 9 0.14893066147335077 1105 10-11 0.1906933731369449 1105 12-13 0.2399614856377923 1105 14-15 0.273451915387561 1105 16-17 0.26503792628000866 1105 18-19 0.24408893450356572 1105 20-21 0.3394046580675081 1105 22-23 0.3623556547379607 1105 24-25 0.3524874858912881 1105 26-27 0.30240128259367083 1105 28-29 0.3630740520355076 1105 30-31 0.3460390498700363 1105 32-33 0.37589637792084574 1105 34-35 0.4162408976432488 1105 36-37 0.35410910266335804 1105 38-39 0.45854608829289845 1105 40-41 0.4315270438222143 1105 42-43 0.5156650577120487 1105 44-45 0.49299598039826975 1105 46-47 0.4778425973965241 1105 48-49 0.4670566620314176 1105 50-51 0.4957579462306043 1105 52-53 0.5211775481322078 1105 54-55 0.5536278941324682 1105 56-57 0.6744167070330533 1105 58-59 0.6223666298632011 1105 60-61 0.6812097453156056 1105 62-63 0.6479360535180021 1105 64-65 0.5822760378323437 1105 66-67 0.6145941738166272 1105 68-69 0.628414124988204 1105 70-71 0.624476276824204 1105 72-73 0.6147526767019826 1105 74-75 0.6332235157616992 1105 76-77 0.5612805861982331 1105 78-79 0.5687045809482996 1105 80-81 0.6163693818882621 1105 82-83 0.6198674948527803 1105 84-85 0.6372740694449988 1105 86-87 0.6620728539306633 1105 88-89 0.698455111762808 1105 90-91 0.683786651091598 1105 92-93 0.678648565849219 1105 94-95 0.7546809327055684 1105 96-97 0.8012053955359804 1105 98-99 0.8029392444347394 1105 100 0.8187445418049961 1106 1 0.1743076746363741 1106 2 0.09936519985522807 1106 3 0.12522244448348374 1106 4 0.14255973835212643 1106 5 0.15721713291895867 1106 6 0.1303607939879825 1106 7 0.16121358974798738 1106 8 0.13197775648072962 1106 9 0.19677154006438258 1106 10-11 0.1675779411599052 1106 12-13 0.16919781376920184 1106 14-15 0.2406030466901754 1106 16-17 0.2070118650754651 1106 18-19 0.1891287310152876 1106 20-21 0.22243178605035752 1106 22-23 0.25128585410809734 1106 24-25 0.22792235901283675 1106 26-27 0.28095869786432814 1106 28-29 0.2443947639717834 1106 30-31 0.22708373765495793 1106 32-33 0.28683148674358705 1106 34-35 0.25137342938211304 1106 36-37 0.24287024607799879 1106 38-39 0.2309125030315542 1106 40-41 0.32063398752636374 1106 42-43 0.36654114150199035 1106 44-45 0.33645209717880675 1106 46-47 0.3352003630249527 1106 48-49 0.39599773875284683 1106 50-51 0.3624613841351447 1106 52-53 0.3858593212212682 1106 54-55 0.44082063357622303 1106 56-57 0.446596227263079 1106 58-59 0.37645204989485137 1106 60-61 0.32310522225136395 1106 62-63 0.29007454128842625 1106 64-65 0.35055080861387466 1106 66-67 0.37792009062105336 1106 68-69 0.37924035425135827 1106 70-71 0.38761202934981753 1106 72-73 0.38691592382868123 1106 74-75 0.3748723081055374 1106 76-77 0.28353664934948597 1106 78-79 0.36406568603467093 1106 80-81 0.3732366441159982 1106 82-83 0.42062558917517023 1106 84-85 0.377721076065086 1106 86-87 0.401288609021595 1106 88-89 0.4472145432823247 1106 90-91 0.43082652436085667 1106 92-93 0.4691846137398663 1106 94-95 0.5061729452503769 1106 96-97 0.567062897184119 1106 98-99 0.5312551638221557 1106 100 0.5509583428610689 1107 1 0.1758440731563411 1107 2 0.08419318186845715 1107 3 0.13882750118402498 1107 4 0.13298979281828593 1107 5 0.14561930654289768 1107 6 0.14437638298122835 1107 7 0.09044277861051597 1107 8 0.1212802926782004 1107 9 0.14767799041054275 1107 10-11 0.1819908461144948 1107 12-13 0.21206927982544954 1107 14-15 0.22662025071910108 1107 16-17 0.21287760333891015 1107 18-19 0.19551816640865383 1107 20-21 0.2551501359517303 1107 22-23 0.17493304282095323 1107 24-25 0.22961148876201776 1107 26-27 0.22391048617438258 1107 28-29 0.24351001318966325 1107 30-31 0.28939072759089157 1107 32-33 0.3043391608889934 1107 34-35 0.30344916160404267 1107 36-37 0.3283267142083872 1107 38-39 0.376375823829747 1107 40-41 0.34195316903235806 1107 42-43 0.36701222111270937 1107 44-45 0.3925328734136002 1107 46-47 0.3532074492914177 1107 48-49 0.32956816205706474 1107 50-51 0.3561549638230943 1107 52-53 0.429435382942863 1107 54-55 0.3818477110307015 1107 56-57 0.4316619380365623 1107 58-59 0.4374213818935573 1107 60-61 0.334704783270908 1107 62-63 0.3968017035091478 1107 64-65 0.29443327445750356 1107 66-67 0.3295908579298583 1107 68-69 0.3406620482315006 1107 70-71 0.36336972575647764 1107 72-73 0.40058854902326857 1107 74-75 0.3403567849461524 1107 76-77 0.2857906120599374 1107 78-79 0.3432886219204292 1107 80-81 0.32748905655078175 1107 82-83 0.3433994261917057 1107 84-85 0.37672150840433005 1107 86-87 0.35735157624988645 1107 88-89 0.37194720580975016 1107 90-91 0.4318300569210223 1107 92-93 0.4722138794884039 1107 94-95 0.40925775263143294 1107 96-97 0.3973505820248384 1107 98-99 0.3569524863751461 1107 100 0.4405985067634006 1108 1 0.09905453613350801 1108 2 0.0024587316292397077 1108 3 0.003431270347704185 1108 4 0.06454744441835203 1108 5 0.1014377565288953 1108 6 0.04217032902543849 1108 7 0.0274371419039241 1108 8 0.057769786961962666 1108 9 0.09423793644026546 1108 10-11 0.10797958466951485 1108 12-13 0.06974700521261212 1108 14-15 0.0476841070427696 1108 16-17 0.06172382175034841 1108 18-19 0.12401684093065768 1108 20-21 0.1233868832977052 1108 22-23 0.06645045607749722 1108 24-25 0.11744537394479693 1108 26-27 0.11697234821821922 1108 28-29 0.16485973623610306 1108 30-31 0.08350968077665044 1108 32-33 0.15801084674519217 1108 34-35 0.19588198457768868 1108 36-37 0.11614488325666628 1108 38-39 0.1725556693203032 1108 40-41 0.2581054656194368 1108 42-43 0.25480295778998396 1108 44-45 0.3400161817134446 1108 46-47 0.3265126185138669 1108 48-49 0.3281241831475583 1108 50-51 0.21993670671406562 1108 52-53 0.3099589034451711 1108 54-55 0.15141319613171333 1108 56-57 0.22094936094010365 1108 58-59 0.16216139640027194 1108 60-61 0.256657640536595 1108 62-63 0.15890761513786344 1108 64-65 0.33607528296059286 1108 66-67 0.324714446902874 1108 68-69 0.29285043334670746 1108 70-71 0.26229757988051716 1108 72-73 0.3157637051846436 1108 74-75 0.33582154631758954 1108 76-77 0.15196567971962693 1108 78-79 0.32493176740238283 1108 80-81 0.35894297851372414 1108 82-83 0.42649336930685067 1108 84-85 0.3246870091269898 1108 86-87 0.34663326701619823 1108 88-89 0.27182566560337307 1108 90-91 0.309145470291579 1108 92-93 0.3604615336427379 1108 94-95 0.3342430522190618 1108 96-97 0.40378301634698843 1108 98-99 0.4321813545520534 1108 100 0.49468342617175765 1109 1 0.14057698514842087 1109 2 0.06778897085067825 1109 3 0.09446853290786095 1109 4 0.09114480788954893 1109 5 0.08985610909250852 1109 6 0.08641836430260952 1109 7 0.10176957650620722 1109 8 0.11296311659192781 1109 9 0.12322036107361356 1109 10-11 0.10138322171460601 1109 12-13 0.1385462586322248 1109 14-15 0.1641313606724495 1109 16-17 0.12812223040510418 1109 18-19 0.15320017878343606 1109 20-21 0.18396761159768715 1109 22-23 0.19626296819229339 1109 24-25 0.16136381860961535 1109 26-27 0.10366429863200466 1109 28-29 0.158609301680805 1109 30-31 0.1845441557884513 1109 32-33 0.22624630262956913 1109 34-35 0.17324533555952826 1109 36-37 0.1623420922212091 1109 38-39 0.16935894091903947 1109 40-41 0.20753242599084842 1109 42-43 0.1763044833535119 1109 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64-65 -0.759109171062299 2316 66-67 -0.7480321959636171 2316 68-69 -0.6598591142579764 2316 70-71 -0.6995418348858422 2316 72-73 -0.6554239804533779 2316 74-75 -0.7248887471076131 2316 76-77 -0.7408278397735906 2316 78-79 -0.7767846739084305 2316 80-81 -0.7399926700301336 2316 82-83 -0.743629105839819 2316 84-85 -0.6611999495071501 2316 86-87 -0.6851197712475532 2316 88-89 -0.6735612914222102 2316 90-91 -0.7688062242768474 2316 92-93 -0.7735044408970992 2316 94-95 -0.7270037275112351 2316 96-97 -0.7652493739345374 2316 98-99 -0.779225924439924 2316 100 -0.810750337662558 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38.0 3 27.0 4 56.0 5 124.0 6 271.0 7 643.0 8 1511.0 9 2904.0 10 5025.0 11 9265.0 12 15262.0 13 21911.0 14 25390.0 15 34216.0 16 47088.0 17 64015.0 18 63251.0 19 38360.0 20 25845.0 21 22062.0 22 23175.0 23 25623.0 24 28608.0 25 31129.0 26 36991.0 27 45812.0 28 58283.0 29 73556.0 30 92630.0 31 116957.0 32 150772.0 33 196814.0 34 279593.0 35 448127.0 36 872538.0 37 1780040.0 38 1681557.0 39 340382.0 40 348.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.95777063117784 18.890651765810603 13.936895879537534 23.21468172347403 2 15.018766829338063 22.945395384935967 36.45934539724787 25.5764923884781 3 19.467554751239952 27.777848697722092 29.858669900616125 22.895926650421828 4 13.75943570745583 19.147458905722207 34.61913616728891 32.47396921953305 5 13.36108725880413 37.600888501980194 33.10378563763635 15.934238601579324 6 30.61040668604647 34.70481888003647 18.065631372275814 16.61914306164125 7 24.796571393737636 33.318734169954986 22.397829254050816 19.486865182256565 8 27.710087941816752 31.410232637192976 21.415246000907782 19.464433420082493 9 28.071533598320407 14.710971849339638 19.79654061387655 37.420953938463406 10-11 24.961356224962252 26.41362645196618 25.663828360529493 22.961188962542074 12-13 27.407350796850448 24.469056023105647 26.198879176035454 21.924714004008443 14-15 25.855376063974344 23.57997618679403 25.40995199822227 25.15469575100936 16-17 22.98093509195751 26.783091148519738 27.486932199988633 22.749041559534117 18-19 24.350240886195742 25.896921398294552 29.002894658252703 20.749943057257 20-21 24.60373206422689 25.449451007160047 28.112185650060194 21.83463127855287 22-23 23.351429577242204 26.474914200439997 25.878151824210548 24.295504398107255 24-25 23.382042512313177 27.21772707214457 25.493807113092647 23.906423302449603 26-27 24.105720756593527 25.701348674688084 26.154085002060008 24.038845566658377 28-29 23.48053908624533 24.91467540919016 28.617304682889827 22.987480821674684 30-31 25.108398332292925 25.036206600783707 27.483105400603673 22.3722896663197 32-33 22.053405636324026 26.42770304023005 28.002659411142833 23.516231912303095 34-35 21.86257805079394 27.01755653005081 28.223089011162916 22.896776407992327 36-37 23.833098407824426 26.81241682980069 26.760681915866012 22.593802846508872 38-39 24.249631563260383 25.30672780877176 26.686929748892435 23.75671087907542 40-41 23.096739030064704 26.31075045776099 26.77291826667177 23.81959224550253 42-43 25.319886042521144 25.419352591310428 26.55613459883352 22.70462676733491 44-45 24.982960278737483 25.187639631309906 27.183811352662207 22.645588737290403 46-47 23.54887462122074 25.683499356948936 28.35741462165467 22.41021140017566 48-49 24.2390352717795 26.469412241109385 27.566812212912023 21.724740274199096 50-51 22.99676399991952 27.205448467989996 26.006850674632144 23.79093685745834 52-53 22.52088980477823 27.520786196632045 24.617333072260823 25.340990926328903 54-55 23.426401600840574 26.638211516714755 24.528357278592047 25.407029603852628 56-57 24.193375553721133 25.157800808248314 25.19222311239956 25.456600525630986 58-59 23.597117937004114 25.087523488344832 27.15048783442098 24.164870740230075 60-61 23.892033333128083 25.799345226035786 26.090785571063428 24.2178358697727 62-63 21.642357443446887 26.356076107042618 26.855502015009353 25.14606443450114 64-65 21.502439252920936 26.449844351646128 26.558602620664356 25.48911377476858 66-67 23.448937953914655 26.209708992383323 25.36398943182747 24.977363621874552 68-69 23.646533545271726 25.158122352756408 25.511036864773214 25.684307237198656 70-71 22.660439715782818 25.79069646452199 25.687103300454634 25.861760519240555 72-73 24.032353952680758 25.088757458640725 25.798576892738588 25.080311695939933 74-75 24.108425265714306 24.705985429210934 26.083129880988732 25.10245942408603 76-77 22.40214230454643 25.759823306420643 26.928819198760383 24.909215190272544 78-79 22.6310210707117 25.540787806949165 26.783670538142584 25.04452058419655 80-81 21.84180232452514 25.73462144299292 26.283321264124393 26.140254968357553 82-83 21.861330943785365 26.044013347515264 25.056553069045645 27.038102639653722 84-85 22.334487828626703 25.599533647463158 24.87381192524696 27.192166598663174 86-87 22.448168494387335 24.389570074303744 25.698029139679402 27.46423229162952 88-89 22.43494540587366 23.767894466145094 26.631587110556605 27.165573017424645 90-91 22.865909482825604 24.287924549577365 26.0079278858328 26.83823808176423 92-93 21.52545002998811 24.747161827466226 26.380438427753656 27.34694971479201 94-95 20.86629989014422 24.972622389573843 26.790696920186807 27.370380800095134 96-97 22.11166308931439 24.4574007049202 25.789825855477172 27.64111035028824 98-99 22.360157706999445 23.882957551268362 25.6851709686152 28.07171377311699 100 22.362577774160908 24.238226502139817 26.039552568568663 27.359643155130613 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 801.0 1 489.0 2 209.5 3 246.5 4 232.0 5 258.5 6 404.5 7 855.5 8 1375.0 9 1478.5 10 1243.0 11 1150.5 12 1332.5 13 1575.0 14 1875.5 15 2213.0 16 2623.0 17 3177.0 18 3825.5 19 4520.0 20 5303.5 21 6272.5 22 7497.0 23 9025.5 24 10862.5 25 13297.5 26 16839.0 27 21030.5 28 26078.0 29 33676.5 30 42557.0 31 51106.5 32 60927.0 33 72142.5 34 84097.0 35 93353.0 36 102348.0 37 113998.0 38 121342.5 39 126854.0 40 129925.0 41 131325.5 42 135360.5 43 147356.0 44 165481.5 45 183768.0 46 209324.0 47 246744.0 48 353737.0 49 457788.0 50 553367.5 51 533346.0 52 386997.5 53 312404.0 54 264744.5 55 225680.5 56 198236.0 57 171738.5 58 153412.5 59 138329.5 60 115889.5 61 92805.5 62 71752.5 63 54940.0 64 40966.0 65 30832.5 66 24009.5 67 18373.5 68 13867.0 69 10684.0 70 8330.5 71 6584.5 72 5366.0 73 5011.5 74 3743.5 75 2847.0 76 2198.0 77 1500.0 78 1071.5 79 603.5 80 429.0 81 355.5 82 285.0 83 225.5 84 150.0 85 93.0 86 56.0 87 25.5 88 15.0 89 10.0 90 5.5 91 2.5 92 3.5 93 2.5 94 1.0 95 1.5 96 1.5 97 1.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.06065884818156334 3 0.025134384122756693 4 0.005870695455195859 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 9.008739828945051E-5 12-13 1.426383806249633E-4 14-15 4.3542242506567746E-4 16-17 4.579442746380401E-4 18-19 0.0 20-21 0.0027776947805913904 22-23 0.005187532684834192 24-25 8.708448501313549E-4 26-27 4.6545155782882764E-4 28-29 0.0050599088705908036 30-31 0.002349779638716501 32-33 0.0011711361777628567 34-35 0.0016966460011179846 36-37 0.004301673268321262 38-39 0.0045644281799988255 40-41 9.609322484208055E-4 42-43 0.002567490851249339 44-45 3.8287144273016466E-4 46-47 0.0022897213731902003 48-49 3.002913276315017E-5 50-51 0.0018768207976968855 52-53 0.007372152093353367 54-55 0.014226301646542394 56-57 0.02590763429140781 58-59 0.027679353124433672 60-61 0.025795025043546 62-63 0.008295547925820235 64-65 0.007139426314438952 66-67 1.8768207976968857E-4 68-69 0.00131377455838782 70-71 1.576529470065384E-4 72-73 8.182938677958421E-4 74-75 0.005517853145228844 76-77 0.0012236871600983695 78-79 0.001163628894572069 80-81 0.0 82-83 0.001246209009670732 84-85 2.2521849572362628E-5 86-87 5.480316729274906E-4 88-89 6.756554871708787E-5 90-91 5.630462393090657E-4 92-93 8.933666997037175E-4 94-95 0.0015540076204930214 96-97 1.8768207976968857E-4 98-99 0.0 100 7.507283190787543E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6660199.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.32100911794592 #Duplication Level Percentage of deduplicated Percentage of total 1 78.19102413017485 29.18167924503785 2 12.594462276066668 9.4007608288142 3 3.7972871618739523 4.251557663752703 4 1.5422904345513015 2.302393414816396 5 0.8421979324479193 1.5715838358002 6 0.5278793592067319 1.1820594226877934 7 0.3716877377248927 0.9710233014061589 8 0.27185781651200014 0.811680643906338 9 0.20271717026344327 0.6809048423789545 >10 1.334903690842634 9.681165818885331 >50 0.1516770502018929 3.9874323667213156 >100 0.1432012686070511 11.238342674168768 >500 0.018482915929869462 4.74662895492238 >1k 0.008232559928737929 5.371212464215097 >5k 8.47469404427903E-4 2.223869049779144 >10k+ 0.0012510262636792851 12.397705472707516 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 93835 1.4088918364150982 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 87434 1.312783597006636 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 52045 0.7814331073290752 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 47233 0.709183013900936 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 42492 0.6379989546858885 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 42421 0.6369329204727967 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 39767 0.5970842612960964 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 34555 0.5188283413153271 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 31649 0.47519601141046985 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 30636 0.4599862556659343 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 29767 0.44693859748034553 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 27653 0.4151978041496958 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 20190 0.30314409524400093 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19265 0.289255621341044 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 17617 0.26451161594420824 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 17280 0.2594517070736175 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 15606 0.2343173229508608 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 15360 0.2306237396209933 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 15318 0.22999312783296716 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 14727 0.22111951910145627 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14261 0.21412273116764227 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 13384 0.20095495645100092 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 12373 0.18577522983922853 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 11933 0.1791688206313355 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 11327 0.17006999340410098 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 11272 0.16924419225311435 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 11169 0.16769769191581213 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 11109 0.16679681793291762 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10876 0.16329842396601063 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 10444 0.15681213128917018 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 10160 0.15254799443680286 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 9044 0.13579173835496508 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 8974 0.13474071870825483 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 8805 0.13220325698976862 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 8760 0.13152760150259774 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 8497 0.1275787705442435 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 7916 0.11885530747654836 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 7817 0.11736886540477244 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 7777 0.11676828274950943 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 7276 0.10924598499234033 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 7159 0.10748928072569604 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 7111 0.10676858153938042 No Hit ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC 6859 0.1029849108112235 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 6823 0.10244438642148679 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.00442929708256465 0.0 0.0 4.5043699144725257E-5 3.002913276315017E-5 2 0.007041831632958715 0.0 0.0 4.5043699144725257E-5 3.002913276315017E-5 3 0.007417195792498092 0.0 0.0 4.5043699144725257E-5 3.002913276315017E-5 4 0.008378128040918897 1.5014566381575086E-5 0.0 4.5043699144725257E-5 4.5043699144725257E-5 5 0.033167177136899366 1.5014566381575086E-5 0.0 2.7026219486835155E-4 4.5043699144725257E-5 6 0.06643945623846975 1.5014566381575086E-5 0.0 6.005826552630034E-4 6.005826552630034E-5 7 0.07390169573011257 1.5014566381575086E-5 0.0 6.756554871708789E-4 6.005826552630034E-5 8 0.08390139694024158 1.5014566381575086E-5 0.0 7.807574518419045E-4 6.005826552630034E-5 9 0.09083812660852926 3.002913276315017E-5 0.0 9.459176820392303E-4 6.005826552630034E-5 10-11 0.16153421241617555 3.002913276315017E-5 0.0 0.0016065586028285342 7.507283190787543E-5 12-13 0.28284440149611145 4.5043699144725257E-5 0.0 0.003738627029012196 9.008739828945051E-5 14-15 0.424626951837325 4.5043699144725257E-5 0.0 0.006426234411314137 1.2011653105260068E-4 16-17 0.48858900462283483 4.5043699144725257E-5 0.0 0.0077550235360835315 1.2011653105260068E-4 18-19 0.5196316206167413 6.005826552630034E-5 0.0 0.008445693589635987 1.2762381424338823E-4 20-21 0.6340801528602974 7.507283190787542E-5 0.0 0.0110281990072669 1.3513109743417578E-4 22-23 0.667697766988644 7.507283190787542E-5 0.0 0.013768357371904353 1.3513109743417578E-4 24-25 0.8229859197900844 7.507283190787542E-5 0.0 0.024939194759796217 1.3513109743417578E-4 26-27 1.1639592150324638 7.507283190787542E-5 0.0 0.0615296930316947 1.3513109743417578E-4 28-29 1.2419373655351738 7.507283190787542E-5 0.0 0.09661873466543566 1.3513109743417578E-4 30-31 1.266103310126319 7.507283190787542E-5 0.0 0.1823444014210386 1.3513109743417578E-4 32-33 1.2876492128838795 7.507283190787542E-5 0.0 0.32255792957537754 1.5014566381575084E-4 34-35 1.304330396133809 7.507283190787542E-5 0.0 0.5055329427844423 1.8017479657890103E-4 36-37 1.3315668195499866 7.507283190787542E-5 1.5014566381575086E-5 0.7757801230864123 1.8768207976968857E-4 38-39 1.3573543373103416 1.0510196467102559E-4 1.5014566381575086E-5 1.168365990265456 1.9518936296047612E-4 40-41 1.379425749891257 1.3513109743417578E-4 1.5014566381575086E-5 1.7228989704361686 1.9518936296047612E-4 42-43 1.4281705396490405 1.3513109743417578E-4 1.5014566381575086E-5 2.1318507149711294 1.9518936296047612E-4 44-45 1.4849931661201115 1.3513109743417578E-4 1.5014566381575086E-5 2.544323375322569 1.9518936296047612E-4 46-47 1.5083783532594146 1.3513109743417578E-4 1.5014566381575086E-5 3.0176200440857697 1.9518936296047612E-4 48-49 1.5247066941993777 1.3513109743417578E-4 1.5014566381575086E-5 3.4552496104095387 2.1020392934205118E-4 50-51 1.5504416609773974 1.3513109743417578E-4 1.5014566381575086E-5 3.9508278956829965 2.1020392934205118E-4 52-53 1.5591426021955201 1.5014566381575084E-4 1.5014566381575086E-5 4.538790807902286 2.2521849572362628E-4 54-55 1.5868144480367627 1.5014566381575084E-4 1.5014566381575086E-5 5.051440655151595 2.4023306210520137E-4 56-57 1.641047061807012 1.5014566381575084E-4 1.5014566381575086E-5 5.582783637545965 2.4023306210520137E-4 58-59 1.6583813786945405 1.5014566381575084E-4 1.5014566381575086E-5 6.126686304718523 2.4023306210520137E-4 60-61 1.6715866898271359 1.576529470065384E-4 1.5014566381575086E-5 6.629283899775367 2.5524762848677646E-4 62-63 1.6824497286042053 1.6516023019732593E-4 1.5014566381575086E-5 7.160604360320164 2.5524762848677646E-4 64-65 1.6910380605744662 1.9518936296047612E-4 1.5014566381575086E-5 7.734746364185215 2.62754911677564E-4 66-67 1.7025767548387067 1.9518936296047612E-4 1.5014566381575086E-5 8.307867377536317 2.7026219486835155E-4 68-69 1.7153241216966642 2.1771121253283873E-4 1.5014566381575086E-5 8.990864987667786 2.7026219486835155E-4 70-71 1.7310668945477454 2.2521849572362628E-4 1.5014566381575086E-5 9.804076424743466 2.7026219486835155E-4 72-73 1.7695191990509591 2.4023306210520137E-4 1.5014566381575086E-5 10.473275648370265 2.852767612499266E-4 74-75 1.8070255858721338 2.4023306210520137E-4 1.5014566381575086E-5 11.120891432823555 3.002913276315017E-4 76-77 1.8192324283403543 2.4774034529598894E-4 1.5014566381575086E-5 11.822131741108636 3.002913276315017E-4 78-79 1.8277531947618983 2.5524762848677646E-4 1.5014566381575086E-5 12.504258506389974 3.002913276315017E-4 80-81 1.8445620018260716 2.62754911677564E-4 1.5014566381575086E-5 13.274483239915202 3.2281317720386435E-4 82-83 1.851085830918866 2.7026219486835155E-4 1.5014566381575086E-5 14.17441130512767 3.6785687634858957E-4 84-85 1.874230784996064 2.7026219486835155E-4 1.5014566381575086E-5 15.003868503028212 3.828714427301647E-4 86-87 1.914154517004672 2.7026219486835155E-4 1.5014566381575086E-5 15.811862378286294 3.9037872592095224E-4 88 1.9238914633031234 2.7026219486835155E-4 1.5014566381575086E-5 16.451985894115175 3.9037872592095224E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATC 2190 0.0 29.408022 3 CGATCTC 2230 0.0 26.551678 5 GATCTAC 3110 0.0 21.326784 1 TCTCGCG 1105 0.0 20.413078 8 CCGATCT 3265 0.0 19.575285 4 AGTACTT 25945 0.0 19.480028 12-13 TCTACAC 4065 0.0 19.428448 3 TACACTC 3980 0.0 18.301004 5 CTCGCGC 1235 0.0 18.264334 9 GTATCAT 2410 0.0 17.957151 1 GAGTACT 24795 0.0 17.834393 12-13 GTATATA 1510 0.0 17.445272 1 CTACACT 5310 0.0 16.99257 4 TACTTTT 30205 0.0 16.865578 14-15 ATCTACA 3985 0.0 16.64399 2 CGCGCCA 1505 0.0 16.565557 1 TAGTACT 2360 0.0 16.129667 4 GTACTTT 28045 0.0 15.885601 14-15 CATGGGG 16715 0.0 15.407301 4 GATGTAA 2415 0.0 15.387804 1 >>END_MODULE