FastQCFastQC Report
Fri 27 May 2016
ERR765448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765448_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13601234
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT795230.5846748905283152No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA765060.5624930796720357No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG305670.22473696136688773No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC224640.16516148461235208No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC223790.16453654131676582No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT213770.15716956270291357No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG198410.14587646973796642No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG190850.14031815054428148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178020.1308851829179617No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC143560.10554924648748783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG45100.020.7405131
GTATAAG54350.019.2862721
CATGGGG301450.018.8936254
CGTTAAC15400.018.313591
TAGTACT54050.018.257934
ATTTAGG61000.016.7214091
GGGCTAT65750.016.6501396
GTACAAG71700.016.5861021
TAGGCAT69100.016.0476865
GGCTATT65050.015.9616837
AGGGCTA63800.015.9078515
CTAGTAC61550.015.8813413
CTAACGC23100.015.868453
TACATGA51250.015.7730922
CATGGGT103750.015.7622814
TTTAGGG68000.015.5509122
GTATTAG49050.015.3328871
CTAGGCA74750.015.2136054
TCTAACG24150.015.1795792
GTACATA36650.015.1339041
Produced by FastQC (version 0.11.5)