##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765448_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13601234
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.728561320244914	33.0	33.0	33.0	27.0	33.0
2	31.653946840411685	33.0	33.0	33.0	27.0	33.0
3	31.599399363322476	33.0	33.0	33.0	27.0	33.0
4	35.225198904746435	37.0	37.0	37.0	33.0	37.0
5	35.443007524170234	37.0	37.0	37.0	33.0	37.0
6	35.423951385587515	37.0	37.0	37.0	33.0	37.0
7	35.282548554050315	37.0	37.0	37.0	33.0	37.0
8	35.264736567285	37.0	37.0	37.0	33.0	37.0
9	34.974067720620056	37.0	37.0	37.0	33.0	37.0
10-11	34.9822614624526	37.0	37.0	37.0	33.0	37.0
12-13	34.960560710888444	37.0	37.0	37.0	33.0	37.0
14-15	36.26445578393843	37.0	37.0	40.0	30.0	40.0
16-17	36.502059666056766	37.0	37.0	40.0	33.0	40.0
18-19	36.317353815102365	37.0	37.0	40.0	33.0	40.0
20-21	36.31354794719361	37.0	37.0	40.0	33.0	40.0
22-23	36.50686812681849	37.0	37.0	40.0	33.0	40.0
24-25	36.52566359052422	37.0	37.0	40.0	33.0	40.0
26-27	36.18853057744614	37.0	37.0	40.0	30.0	40.0
28-29	36.0719526257691	37.0	37.0	40.0	27.0	40.0
30-31	35.873329287621985	37.0	37.0	40.0	27.0	40.0
32-33	35.82564273212269	37.0	37.0	40.0	27.0	40.0
34-35	35.778390806304785	37.0	37.0	40.0	27.0	40.0
36-37	35.55386992827268	37.0	37.0	40.0	27.0	40.0
38-39	35.41924798882219	37.0	35.0	40.0	27.0	40.0
40-41	35.561253853878256	37.0	37.0	40.0	27.0	40.0
42-43	35.48278174612686	37.0	33.0	40.0	27.0	40.0
44-45	35.43090483554654	37.0	33.0	40.0	27.0	40.0
46-47	35.390172024097225	37.0	33.0	40.0	27.0	40.0
48-49	35.22696584001128	37.0	33.0	40.0	27.0	40.0
50-51	35.08011637032346	37.0	33.0	40.0	27.0	40.0
52-53	34.97145858236098	37.0	33.0	40.0	27.0	40.0
54-55	34.8408301408534	37.0	33.0	40.0	27.0	40.0
56-57	34.62716908627556	37.0	33.0	40.0	27.0	40.0
58-59	34.432654198876364	37.0	33.0	38.5	27.0	40.0
60-61	34.15014909676578	37.0	33.0	37.0	27.0	40.0
62-63	33.90296064312988	37.0	33.0	37.0	27.0	40.0
64-65	33.70862673195683	37.0	33.0	37.0	27.0	40.0
66-67	33.48539672944382	37.0	33.0	37.0	27.0	40.0
68-69	33.27491976095698	37.0	33.0	37.0	27.0	40.0
70-71	33.01886894968501	37.0	33.0	37.0	22.0	37.0
72-73	32.75918670320649	37.0	33.0	37.0	22.0	37.0
74-75	32.531962871898244	35.0	33.0	37.0	22.0	37.0
76-77	32.31601242946044	33.0	33.0	37.0	22.0	37.0
78-79	32.1165079947893	33.0	33.0	37.0	22.0	37.0
80-81	31.976591351931745	33.0	33.0	37.0	22.0	37.0
82-83	31.84328157283376	33.0	33.0	37.0	22.0	37.0
84-85	31.67499493060703	33.0	33.0	37.0	22.0	37.0
86-87	31.459948045890542	33.0	33.0	37.0	22.0	37.0
88-89	31.3089139191341	33.0	33.0	37.0	22.0	37.0
90-91	31.212725073327906	33.0	27.0	37.0	22.0	37.0
92-93	31.097594931459895	33.0	27.0	37.0	22.0	37.0
94-95	30.971702641098595	33.0	27.0	37.0	15.0	37.0
96-97	30.869298734217793	33.0	27.0	37.0	15.0	37.0
98-99	30.731186082086374	33.0	27.0	37.0	15.0	37.0
100	30.632187050086777	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	5.0
7	217.0
8	1295.0
9	2791.0
10	4314.0
11	7639.0
12	13391.0
13	22147.0
14	32197.0
15	38641.0
16	44512.0
17	52751.0
18	62163.0
19	74257.0
20	86771.0
21	102794.0
22	119138.0
23	125889.0
24	132715.0
25	147274.0
26	172892.0
27	209383.0
28	259367.0
29	325373.0
30	405790.0
31	505770.0
32	651069.0
33	856637.0
34	1130556.0
35	1534726.0
36	2269617.0
37	3095177.0
38	1113277.0
39	699.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49687327184771	18.311400556774903	12.59731534046816	25.594410830909233
2	15.52761933281595	20.65621328262768	37.50408241473333	26.31208496982304
3	18.05255477372626	26.18062482479438	29.11306318403731	26.653757217442053
4	12.741552297312422	17.366629715681363	36.33823726886085	33.55358071814536
5	14.09914402103776	37.18381700760179	33.49995139407814	15.217087577282312
6	31.99981614672962	35.75629145891247	17.507302651899572	14.736589742458339
7	27.46813677244637	31.53939470974944	22.30982764713342	18.682640870670767
8	27.455461229884598	32.618979238695815	20.16324081104097	19.762318720378623
9	27.32189527610315	14.357909215996436	19.626705374540887	38.693490133359525
10-11	25.22573131697069	25.126620662314302	26.93881266244488	22.708835358270125
12-13	26.700634369814054	22.781788209548715	26.423556639752338	24.094020780884897
14-15	24.608222548319215	23.43760402551899	24.36034610722745	27.593827318934345
16-17	23.92518874482718	26.046479173098046	25.686601254170267	24.341730827904502
18-19	24.545659450066168	25.372485485619766	27.154544001062895	22.927311063251167
20-21	25.12352460904033	24.907960398008136	26.415575328577884	23.55293966437365
22-23	24.526057372167774	24.8481416133803	25.647625596179775	24.97817541827216
24-25	24.20052441434521	25.53564499291082	25.617661722705794	24.646168870038178
26-27	24.666952357404558	24.83946994782994	25.87580366147957	24.61777403328594
28-29	24.163202406016275	24.818059656558063	26.58281166227653	24.43592627514913
30-31	24.887409693670662	24.889090393012232	26.172096551432965	24.051403361884134
32-33	23.945534125204727	25.231915394786757	25.909077786773576	24.91347269323494
34-35	24.11087590652415	25.334378243891187	26.494006819085907	24.060739030498755
36-37	24.83977838131802	24.9790743443978	25.923741868747562	24.257405405536613
38-39	25.037861789049394	24.599582730441238	25.909833273508486	24.45272220700088
40-41	24.38775610787386	25.04186719987548	26.15574510080568	24.414631591444984
42-43	25.149403674525043	24.849818377701187	25.700804964447048	24.29997298332672
44-45	24.880724816171718	25.023966487415034	25.441126635855525	24.654182060557723
46-47	24.303926296838647	25.053716875237324	26.314804044539624	24.327552783384405
48-49	25.188087360318978	25.168543475524384	25.572259145806065	24.071110018350577
50-51	24.045942782418816	25.68711366990287	25.369926294588502	24.89701725308981
52-53	23.87487698227593	25.6645401738914	24.7908188625106	25.669763981322074
54-55	24.24101004364751	25.590424368847707	24.808388709436215	25.360176878068565
56-57	24.706625884092574	24.77209788464782	24.810969357633287	25.710306873626322
58-59	24.215398396939573	24.833022503693414	25.678725180377015	25.272853918989995
60-61	24.52088170823324	25.20813185038946	25.249936880727148	25.02104956065016
62-63	23.736908332197096	25.329897401034273	25.198052033868727	25.735142232899904
64-65	23.508580563897137	25.240022868563695	25.48430271320098	25.767093854338192
66-67	24.624261447325214	24.963250429861617	25.144348694170237	25.268139428642932
68-69	24.658167003950688	24.682874656106502	25.06586348354956	25.59309485639325
70-71	23.90383059529625	24.979465089475745	25.260796304568974	25.85590801065904
72-73	24.758975186956025	24.944969900802082	25.1290089038513	25.167046008390585
74-75	24.948304288768107	24.786028464347883	25.083196550310728	25.18247069657329
76-77	24.33640025308034	25.048755756333378	25.607422778311452	25.00742121227483
78-79	24.53554581885732	25.216634755346462	25.329948003247356	24.917871422548863
80-81	23.906186839333454	25.25366463287942	25.598916597665532	25.241231930121593
82-83	23.971758963482674	25.27100457051567	24.96013862893941	25.79709783706225
84-85	24.192555342761622	25.492274276350308	24.578973867911433	25.73619651297664
86-87	24.249504538314707	24.867417655056467	24.922256750269113	25.960821056359716
88-89	24.242979576713154	24.50733763641543	25.279835928160928	25.96984685871049
90-91	24.565945262833452	24.784731731533896	25.03830316430679	25.61101984132586
92-93	24.051641329665824	24.982615778729844	25.00378381384981	25.961959077754514
94-95	23.362410964093154	25.151841802630763	25.49511489480093	25.99063233847515
96-97	24.348406578869344	24.867158690171323	24.9399516216141	25.844483109345234
98-99	24.19288066612314	24.937693320001145	24.791318162044757	26.07810785183096
100	23.911974169804097	25.14588452201359	24.886407634359855	26.055733673822456
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	985.0
1	999.5
2	1410.5
3	1789.0
4	1492.5
5	1331.0
6	1733.5
7	3091.5
8	5070.5
9	5118.0
10	3440.5
11	2433.0
12	2232.5
13	2366.0
14	2676.5
15	3218.5
16	3839.0
17	4475.0
18	5316.5
19	6369.0
20	7646.0
21	9479.5
22	12128.0
23	15399.0
24	19167.0
25	23744.0
26	30438.0
27	38226.5
28	46919.5
29	60291.5
30	76312.0
31	91743.0
32	110772.0
33	130758.0
34	148943.0
35	166025.5
36	183132.5
37	207132.5
38	225615.0
39	236062.5
40	244983.5
41	253838.0
42	270652.0
43	304464.5
44	348233.0
45	395436.5
46	453878.5
47	511160.5
48	578788.0
49	657772.5
50	780499.0
51	785010.0
52	657333.0
53	608834.0
54	592791.0
55	559080.5
56	526727.5
57	487801.0
58	453309.0
59	417565.5
60	360296.5
61	298875.0
62	245318.5
63	194710.0
64	148356.0
65	116277.0
66	89999.0
67	67937.0
68	53435.0
69	43106.0
70	36526.0
71	31848.5
72	26987.0
73	25633.5
74	21449.5
75	16232.0
76	12027.5
77	8241.0
78	5809.0
79	3763.0
80	2447.0
81	1697.5
82	1134.0
83	711.5
84	513.5
85	342.5
86	201.5
87	135.5
88	86.5
89	49.0
90	29.0
91	21.5
92	15.0
93	10.5
94	7.0
95	4.5
96	4.0
97	3.5
98	2.0
99	2.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04787800871597386
2	0.024093402113367066
3	0.021512753916299067
4	0.022350913159791236
5	0.015204502767910617
6	0.02524770914168523
7	0.04827503151552278
8	0.00614650111894259
9	0.004087864380540766
10-11	0.00201819923104036
12-13	0.0013013525096325818
14-15	0.002238767453011984
16-17	0.006819234195956044
18-19	0.004929699761065797
20-21	0.00816102421295009
22-23	0.018104974886837474
24-25	0.02498670341235214
26-27	0.02779159596842463
28-29	0.043973951187076116
30-31	0.04193001899680573
32-33	0.0395662628846765
34-35	0.04742216772389917
36-37	0.04470550245661533
38-39	0.054285515564249535
40-41	0.05190705490398886
42-43	0.057174959272077816
44-45	0.05715290244988065
46-47	0.062042164703584986
48-49	0.06431034125285985
50-51	0.03337197198430671
52-53	3.198239218588548E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.403921438304789E-4
64-65	2.2056822197162403E-4
66-67	4.006989365817837E-4
68-69	0.0013564945651254878
70-71	4.411364439432481E-5
72-73	3.014432366945529E-4
74-75	6.617046659148722E-5
76-77	1.4336934428155562E-4
78-79	0.0
80-81	5.881819252576641E-5
82-83	2.463011812016468E-4
84-85	0.0013087047836983028
86-87	0.003937142762193489
88-89	0.0026651993488237906
90-91	0.003896705254832025
92-93	0.003959199584390651
94-95	0.0029482619003540415
96-97	0.002393165208392121
98-99	0.004157710984165113
100	0.002999727818814087
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.3601234E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.58865969756508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.57035188044149	24.755225456275774
2	13.298578685881859	9.199600252545197
3	5.375599756345874	5.5780437192768355
4	2.698055746331536	3.7328852821968592
5	1.5952875275721448	2.7589428705481445
6	1.0118709630330078	2.09995562389177
7	0.7358532295179281	1.7816523860204905
8	0.5226034570728711	1.4460922506771678
9	0.40627653553609666	1.2647304747687376
>10	2.4081489954649005	15.633694428184594
>50	0.2077148020305405	4.957883563248141
>100	0.1358991335572813	9.371841508956145
>500	0.019778148492005463	4.744207637544635
>1k	0.013023834957808116	8.31196461768367
>5k	6.45623469252265E-4	1.5253091118938849
>10k+	3.116802955010935E-4	2.837970816288062
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	79523	0.5846748905283152	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	76506	0.5624930796720357	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	30567	0.22473696136688773	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	22464	0.16516148461235208	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	22379	0.16453654131676582	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	21377	0.15716956270291357	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	19841	0.14587646973796642	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	19085	0.14031815054428148	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17802	0.1308851829179617	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	14356	0.10554924648748783	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4704548131441603E-5
2	0.0	0.0	0.0	0.0	1.4704548131441603E-5
3	0.0	0.0	0.0	0.0	1.4704548131441603E-5
4	0.0	0.0	0.0	0.0	1.4704548131441603E-5
5	0.0	0.0	0.0	0.0	1.4704548131441603E-5
6	0.0	0.0	0.0	0.0	1.4704548131441603E-5
7	0.0	0.0	0.0	0.0	2.2056822197162404E-5
8	0.0	0.0	0.0	0.0	2.2056822197162404E-5
9	0.0	0.0	0.0	1.4704548131441603E-5	3.676137032860401E-5
10-11	0.0	1.4704548131441603E-5	0.0	2.2056822197162404E-5	3.676137032860401E-5
12-13	3.6761370328604007E-6	1.4704548131441603E-5	0.0	3.676137032860401E-5	4.411364439432481E-5
14-15	7.3522740657208015E-6	1.4704548131441603E-5	0.0	4.7789781427185206E-5	5.881819252576641E-5
16-17	7.3522740657208015E-6	1.4704548131441603E-5	0.0	7.719887769006841E-5	8.822728878864962E-5
18-19	1.4704548131441603E-5	1.4704548131441603E-5	0.0	9.557956285437043E-5	8.822728878864962E-5
20-21	1.4704548131441603E-5	1.4704548131441603E-5	0.0	2.3159663307020524E-4	9.557956285437041E-5
22-23	2.2056822197162404E-5	2.2056822197162404E-5	0.0	9.668240396422854E-4	1.1396024801867241E-4
24-25	3.30852332957436E-5	2.5732959230022805E-5	0.0	0.00302913691507697	1.2498865911725362E-4
26-27	5.146591846004561E-5	2.9409096262883206E-5	0.0	0.005161296394136003	1.2498865911725362E-4
28-29	5.5142055492906014E-5	2.9409096262883206E-5	0.0	0.01598016768184416	1.2498865911725362E-4
30-31	6.249432955862682E-5	2.9409096262883206E-5	0.0	0.047128076761270336	1.2498865911725362E-4
32-33	9.190342582151003E-5	2.9409096262883206E-5	0.0	0.10308991081250421	1.2498865911725362E-4
34-35	9.557956285437043E-5	3.30852332957436E-5	0.0	0.1766530889770737	1.3234093318297444E-4
36-37	9.557956285437043E-5	4.411364439432481E-5	0.0	0.29091110409540777	1.3234093318297444E-4
38-39	9.557956285437043E-5	4.7789781427185206E-5	0.0	0.46999411965120225	1.3969320724869523E-4
40-41	9.557956285437043E-5	5.146591846004561E-5	0.0	0.6998629683159631	1.3969320724869523E-4
42-43	1.0293183692009122E-4	5.146591846004561E-5	0.0	0.8978964702761529	1.4336934428155565E-4
44-45	1.1396024801867243E-4	5.146591846004561E-5	0.0	1.1263316254980982	1.4704548131441603E-4
46-47	1.2131252208439322E-4	5.146591846004561E-5	0.0	1.3862051046250656	1.6175002944585765E-4
48-49	1.2498865911725362E-4	5.146591846004561E-5	0.0	1.6547358864644193	1.7645457757729924E-4
50-51	1.2498865911725362E-4	5.146591846004561E-5	0.0	1.9480144228089893	1.7645457757729924E-4
52-53	1.2498865911725362E-4	5.146591846004561E-5	0.0	2.2463697043959394	1.9115912570874082E-4
54-55	1.2498865911725362E-4	5.146591846004561E-5	0.0	2.5386042178231767	2.0953981087304283E-4
56-57	1.2498865911725362E-4	5.146591846004561E-5	0.0	2.8651481181781007	2.1689208493876365E-4
58-59	1.2498865911725362E-4	5.146591846004561E-5	0.0	3.2249206211730495	2.2056822197162406E-4
60-61	1.2498865911725362E-4	5.146591846004561E-5	0.0	3.5832189932178213	2.2792049603734485E-4
62-63	1.2498865911725362E-4	5.146591846004561E-5	0.0	3.9490093325355624	2.2792049603734485E-4
64-65	1.2498865911725362E-4	5.881819252576641E-5	0.0	4.33742629529056	2.3159663307020524E-4
66-67	1.2498865911725362E-4	5.881819252576641E-5	0.0	4.760891548516848	2.3527277010306565E-4
68-69	1.2498865911725362E-4	5.881819252576641E-5	0.0	5.226088309340167	2.3527277010306565E-4
70-71	1.2498865911725362E-4	5.881819252576641E-5	0.0	5.766142248563623	2.3894890713592606E-4
72-73	1.2498865911725362E-4	5.881819252576641E-5	0.0	6.270541334705365	2.646818663659489E-4
74-75	1.2498865911725362E-4	5.881819252576641E-5	0.0	6.7638862767892975	2.646818663659489E-4
76-77	1.3234093318297444E-4	5.881819252576641E-5	0.0	7.292636094636707	2.6835800339880924E-4
78-79	1.3234093318297444E-4	6.617046659148722E-5	0.0	7.839516620330185	2.7938641449739047E-4
80-81	1.3234093318297444E-4	6.617046659148722E-5	0.0	8.426014874826798	2.867386885631113E-4
82-83	1.3234093318297444E-4	6.617046659148722E-5	0.0	9.055759205377981	2.9409096262883206E-4
84-85	1.3234093318297444E-4	6.617046659148722E-5	0.0	9.66653834497664	2.9409096262883206E-4
86-87	1.3234093318297444E-4	7.352274065720801E-5	0.0	10.291294157574232	2.9409096262883206E-4
88	1.3234093318297444E-4	8.087501472292882E-5	0.0	10.791631112294663	3.014432366945529E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	4510	0.0	20.740513	1
GTATAAG	5435	0.0	19.286272	1
CATGGGG	30145	0.0	18.893625	4
CGTTAAC	1540	0.0	18.31359	1
TAGTACT	5405	0.0	18.25793	4
ATTTAGG	6100	0.0	16.721409	1
GGGCTAT	6575	0.0	16.650139	6
GTACAAG	7170	0.0	16.586102	1
TAGGCAT	6910	0.0	16.047686	5
GGCTATT	6505	0.0	15.961683	7
AGGGCTA	6380	0.0	15.907851	5
CTAGTAC	6155	0.0	15.881341	3
CTAACGC	2310	0.0	15.86845	3
TACATGA	5125	0.0	15.773092	2
CATGGGT	10375	0.0	15.762281	4
TTTAGGG	6800	0.0	15.550912	2
GTATTAG	4905	0.0	15.332887	1
CTAGGCA	7475	0.0	15.213605	4
TCTAACG	2415	0.0	15.179579	2
GTACATA	3665	0.0	15.133904	1
>>END_MODULE