FastQCFastQC Report
Fri 27 May 2016
ERR765447_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765447_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7346125
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA440760.5999897905358267No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT428530.5833415576239174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT373400.508295189640797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245620.33435314536575406No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG236280.3216389593152853No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA193950.26401674352124416No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC187270.25492351409756847No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG183020.2491381510660382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177430.24152869710221378No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG170450.23202708911160647No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT149760.20386258061222753No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC145410.19794109139172011No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC138740.1888614745869421No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT130080.17707294662151815No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC115600.15736187445762223No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG87200.11870203678810258No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA82060.11170515067467543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC86150.023.9975451
AGTACTT380150.022.31502712-13
GAGTACT371550.021.93977712-13
ATCTACA98300.021.1559052
TCTACAC112050.020.4880053
TACACTC111450.020.1791025
GTACTAG17350.020.0858331
GTACTTT421150.019.43379614-15
TACTTTT443350.019.26608714-15
CTACACT131900.018.7987384
GTATAAG22600.018.1287731
GTATCAA1226700.017.8322011
ACTTTTT468900.017.63361216-17
CATGGGG163800.017.1483884
TAGTACT19100.016.9972484
CATGGGT89100.016.8451884
CACTCTT137750.016.0734737
AACGCAG1356200.016.0450446
CAACGCA1357500.015.9153435
ATGGGTA52300.015.7431045
Produced by FastQC (version 0.11.5)