##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765447_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7346125
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.306322040531576	33.0	31.0	34.0	31.0	34.0
2	32.341382974016916	34.0	31.0	34.0	31.0	34.0
3	32.364122445506986	34.0	31.0	34.0	30.0	34.0
4	35.822955912130546	37.0	35.0	37.0	35.0	37.0
5	35.8422587418537	37.0	35.0	37.0	35.0	37.0
6	35.93131766747775	37.0	37.0	37.0	35.0	37.0
7	35.885492419472854	37.0	37.0	37.0	35.0	37.0
8	35.912971396484544	37.0	37.0	37.0	35.0	37.0
9	37.62220204529599	39.0	38.0	39.0	35.0	39.0
10-11	37.55158358998792	39.0	38.0	39.0	35.0	39.0
12-13	37.520329561503516	39.0	38.0	39.0	35.0	39.0
14-15	38.89610842450952	41.0	39.0	41.0	36.0	41.0
16-17	38.86684728343174	41.0	39.0	41.0	36.0	41.0
18-19	38.88129769095952	41.0	39.0	41.0	35.0	41.0
20-21	38.85320716704385	41.0	39.0	41.0	35.0	41.0
22-23	38.74181646786571	41.0	39.0	41.0	35.0	41.0
24-25	38.65068910480015	41.0	38.5	41.0	35.0	41.0
26-27	38.51957596692134	40.0	38.0	41.0	34.5	41.0
28-29	38.358897173680006	40.0	38.0	41.0	34.0	41.0
30-31	38.10988807024111	40.0	38.0	41.0	34.0	41.0
32-33	37.84677785907536	40.0	37.0	41.0	33.5	41.0
34-35	37.65266463611768	40.0	37.0	41.0	33.0	41.0
36-37	37.45604655515663	40.0	37.0	41.0	33.0	41.0
38-39	37.227648181864595	40.0	36.5	41.0	32.5	41.0
40-41	36.941007742177	40.0	36.0	41.0	31.5	41.0
42-43	36.6108415661318	40.0	35.5	41.0	31.0	41.0
44-45	36.17799159420783	40.0	35.0	41.0	30.0	41.0
46-47	35.64809719409893	39.0	35.0	41.0	27.5	41.0
48-49	35.16444642583675	39.0	35.0	41.0	25.5	41.0
50-51	33.833068318331094	38.0	33.5	40.0	23.0	40.5
52-53	33.720793615681735	38.0	33.5	39.5	22.0	40.0
54-55	34.478145280675186	39.0	35.0	40.5	23.0	41.0
56-57	34.46211376746244	38.5	35.0	41.0	22.0	41.0
58-59	34.22231121849955	38.0	35.0	41.0	21.5	41.0
60-61	33.88334380370603	37.0	35.0	40.0	20.0	41.0
62-63	33.518778607769406	37.0	34.0	40.0	18.0	41.0
64-65	33.17456393676939	36.0	34.0	39.5	15.5	41.0
66-67	32.849931086116825	36.0	34.0	39.0	12.0	41.0
68-69	32.594525481120996	35.0	34.0	39.0	7.5	41.0
70-71	32.267672003947666	35.0	34.0	37.5	4.5	40.5
72-73	31.919237897531012	35.0	34.0	37.0	2.0	39.5
74-75	31.559213735132467	35.0	33.5	37.0	2.0	39.0
76-77	31.211636815327807	35.0	33.0	36.0	2.0	39.0
78-79	30.881385220099034	35.0	33.0	36.0	2.0	37.0
80-81	30.567477683812896	35.0	33.0	35.0	2.0	37.0
82-83	30.29794851026902	35.0	33.0	35.0	2.0	36.5
84-85	30.03135768857731	35.0	32.5	35.0	2.0	36.0
86-87	29.783186101516108	35.0	32.0	35.0	2.0	36.0
88-89	29.54200125916725	35.0	32.0	35.0	2.0	36.0
90-91	29.289656111215095	35.0	32.0	35.0	2.0	35.0
92-93	29.010417396926954	35.0	31.0	35.0	2.0	35.0
94-95	28.672477734860216	35.0	31.0	35.0	2.0	35.0
96-97	28.364981946264187	35.0	31.0	35.0	2.0	35.0
98-99	28.063664040565605	35.0	30.0	35.0	2.0	35.0
100	27.537264884547977	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4575906114300352
1101	2	0.5457724870839868
1101	3	0.5695163460686032
1101	4	0.48217633296253126
1101	5	0.5136780248446158
1101	6	0.48484146769913394
1101	7	0.5032038000525247
1101	8	0.4310835499189807
1101	9	0.5527128609629557
1101	10-11	0.5215225351400292
1101	12-13	0.5893946181263843
1101	14-15	0.7124251897357752
1101	16-17	0.7198664961356727
1101	18-19	0.692162494477131
1101	20-21	0.7182391773231771
1101	22-23	0.7663613653900327
1101	24-25	0.6949592039646646
1101	26-27	0.7158991223472313
1101	28-29	0.7492473624560674
1101	30-31	0.746532640238847
1101	32-33	0.7797480306208868
1101	34-35	0.8105812532547034
1101	36-37	0.8592414884978155
1101	38-39	0.9220865814697206
1101	40-41	0.9358202130640692
1101	42-43	0.8335087690459773
1101	44-45	0.8300633313076347
1101	46-47	0.8489789889194697
1101	48-49	0.9379960205391953
1101	50-51	0.9351650009459576
1101	52-53	0.9759963926455981
1101	54-55	1.0717324770815821
1101	56-57	1.0480394055678275
1101	58-59	1.0595027071032632
1101	60-61	1.0838846709268068
1101	62-63	1.1073995281699638
1101	64-65	1.116413526961928
1101	66-67	1.1334930648917023
1101	68-69	1.1080918212316178
1101	70-71	1.0261015846651134
1101	72-73	1.0138007930930115
1101	74-75	0.963253425519472
1101	76-77	0.9428328074771137
1101	78-79	0.994849387122219
1101	80-81	0.9705926157365532
1101	82-83	0.884258215709405
1101	84-85	0.8229844118995295
1101	86-87	0.9007924471117867
1101	88-89	0.887487666513298
1101	90-91	0.8133032603149815
1101	92-93	0.8180372686407793
1101	94-95	0.7862666146507706
1101	96-97	0.7444474349474355
1101	98-99	0.7441521684802339
1101	100	0.7875038553889055
1102	1	0.4461917741110071
1102	2	0.5711509074350758
1102	3	0.608836504231796
1102	4	0.45593694939342555
1102	5	0.44264173913980187
1102	6	0.4162287675082581
1102	7	0.4540026216058166
1102	8	0.3993095359695005
1102	9	0.418488480879617
1102	10-11	0.4894454176432603
1102	12-13	0.49591596163418217
1102	14-15	0.673382889437427
1102	16-17	0.6624959702104647
1102	18-19	0.6689128832839373
1102	20-21	0.6723192540178502
1102	22-23	0.6686614909721555
1102	24-25	0.6741701379644951
1102	26-27	0.6452890303693621
1102	28-29	0.6137418222483362
1102	30-31	0.6395661400010368
1102	32-33	0.6849864429225576
1102	34-35	0.7289991391169437
1102	36-37	0.6873808605735263
1102	38-39	0.7285729259169642
1102	40-41	0.7976126080453767
1102	42-43	0.7697084552165165
1102	44-45	0.7744228415084606
1102	46-47	0.7824609035806134
1102	48-49	0.8102199625749833
1102	50-51	0.6903616421298651
1102	52-53	0.7489877754630214
1102	54-55	0.9366539850700448
1102	56-57	0.9536104549812876
1102	58-59	0.9407730860007177
1102	60-61	0.9035932279330012
1102	62-63	0.8769060901986307
1102	64-65	0.9318411930170711
1102	66-67	0.896500782364761
1102	68-69	0.8559182456528376
1102	70-71	0.8776897058770814
1102	72-73	0.8295802052995782
1102	74-75	0.7866470909070138
1102	76-77	0.747241238062248
1102	78-79	0.7712764043710933
1102	80-81	0.7664800925140192
1102	82-83	0.8449147693751549
1102	84-85	0.7621509240626203
1102	86-87	0.804832365305213
1102	88-89	0.7756153475853544
1102	90-91	0.8432633758653267
1102	92-93	0.8941985055592845
1102	94-95	0.8245750035409181
1102	96-97	0.8831021914934531
1102	98-99	0.885083868080784
1102	100	0.869894887799358
1103	1	0.4756144203898529
1103	2	0.5160697385428321
1103	3	0.5473024519303706
1103	4	0.42569868174766157
1103	5	0.44321039074175417
1103	6	0.42475320496090774
1103	7	0.4332368648604543
1103	8	0.42212200105757347
1103	9	0.4648675305648098
1103	10-11	0.515600941756766
1103	12-13	0.5233415034869822
1103	14-15	0.6429626095366174
1103	16-17	0.6126700607112809
1103	18-19	0.6073350448091688
1103	20-21	0.5487134237117104
1103	22-23	0.65970172884699
1103	24-25	0.5797278675188693
1103	26-27	0.5887817707442977
1103	28-29	0.5993569051284311
1103	30-31	0.603831023516264
1103	32-33	0.6496822331247714
1103	34-35	0.6330921533719831
1103	36-37	0.6067101508814616
1103	38-39	0.6528079298524716
1103	40-41	0.7311547327499639
1103	42-43	0.7123017199844526
1103	44-45	0.7629030536137549
1103	46-47	0.7508905914285009
1103	48-49	0.7796359110128108
1103	50-51	0.7303572713861683
1103	52-53	0.7218961195854234
1103	54-55	0.8398615212959939
1103	56-57	0.8467300243038096
1103	58-59	0.8690220266246556
1103	60-61	0.8654311064111013
1103	62-63	0.9027026639240034
1103	64-65	0.8758127380408496
1103	66-67	0.8574486336009599
1103	68-69	0.9517747088900776
1103	70-71	0.8710310580728517
1103	72-73	0.8028834123458921
1103	74-75	0.7898362640604581
1103	76-77	0.8141540783816446
1103	78-79	0.822544458075253
1103	80-81	0.8590728017530971
1103	82-83	0.7900525313496694
1103	84-85	0.8192139312725111
1103	86-87	0.819728718927422
1103	88-89	0.8656641282694686
1103	90-91	0.8388741291196098
1103	92-93	0.7990498823391761
1103	94-95	0.760848318147243
1103	96-97	0.8479925042859513
1103	98-99	0.8523283110204893
1103	100	0.7700079969772275
1104	1	0.4245611588845293
1104	2	0.5095192468786109
1104	3	0.47627721286596625
1104	4	0.422490620866121
1104	5	0.4406946875925897
1104	6	0.43468033512990445
1104	7	0.4470098617604279
1104	8	0.38242471888980845
1104	9	0.430472691419034
1104	10-11	0.4695351169117785
1104	12-13	0.5085617167233139
1104	14-15	0.6378228787850233
1104	16-17	0.6279746940118827
1104	18-19	0.595007057560359
1104	20-21	0.5676676600140595
1104	22-23	0.6596396709530907
1104	24-25	0.6994153549575799
1104	26-27	0.7058768542661014
1104	28-29	0.7502778475552745
1104	30-31	0.7659730421656192
1104	32-33	0.7458235282438892
1104	34-35	0.6896402250654674
1104	36-37	0.6295378990043403
1104	38-39	0.7432287012407031
1104	40-41	0.7236059826443508
1104	42-43	0.827838260312248
1104	44-45	0.8038924360946496
1104	46-47	0.6827583248510862
1104	48-49	0.6722268219308134
1104	50-51	0.5961288770080415
1104	52-53	0.6967149294027948
1104	54-55	0.6886127343575978
1104	56-57	0.6618666952279852
1104	58-59	0.6231774445289915
1104	60-61	0.6987107902234655
1104	62-63	0.6707003145015591
1104	64-65	0.6067441888830416
1104	66-67	0.6227950626675138
1104	68-69	0.6542152288802683
1104	70-71	0.6370115604915938
1104	72-73	0.6217406037196298
1104	74-75	0.6421422911001464
1104	76-77	0.5984849318602095
1104	78-79	0.6056005612004576
1104	80-81	0.6841892269559011
1104	82-83	0.7321252814126247
1104	84-85	0.6993796049308401
1104	86-87	0.6715647384282839
1104	88-89	0.572455837695788
1104	90-91	0.5747161107721297
1104	92-93	0.6048074849438834
1104	94-95	0.5607999187323003
1104	96-97	0.5542545682502897
1104	98-99	0.586827578056468
1104	100	0.6756028873115376
1105	1	0.341540473655499
1105	2	0.39771499245463815
1105	3	0.3903167050791012
1105	4	0.3516775074516545
1105	5	0.3726168351659922
1105	6	0.36662014286972067
1105	7	0.3105983638503744
1105	8	0.3640561295970173
1105	9	0.3772274840165295
1105	10-11	0.4124812164806215
1105	12-13	0.4547887448428227
1105	14-15	0.5705224259122801
1105	16-17	0.585087307816103
1105	18-19	0.5546598445993993
1105	20-21	0.50903470507469
1105	22-23	0.539082059093424
1105	24-25	0.5071504592630873
1105	26-27	0.4794757556920075
1105	28-29	0.46578427529713196
1105	30-31	0.5677674195281526
1105	32-33	0.560076995114315
1105	34-35	0.5896029856774163
1105	36-37	0.5783894537670093
1105	38-39	0.5455430575470714
1105	40-41	0.5512626291706582
1105	42-43	0.5699228012535897
1105	44-45	0.5580776161785437
1105	46-47	0.5292128703277186
1105	48-49	0.6511982156191678
1105	50-51	0.5846087156325055
1105	52-53	0.6333241264385094
1105	54-55	0.6573195632063005
1105	56-57	0.6302297216898154
1105	58-59	0.6607778335870762
1105	60-61	0.6546622055752778
1105	62-63	0.648715253496249
1105	64-65	0.6438596278280571
1105	66-67	0.6127673432508729
1105	68-69	0.5721032405908701
1105	70-71	0.5390045479237244
1105	72-73	0.5605825607770072
1105	74-75	0.5320118536454324
1105	76-77	0.5041748941568471
1105	78-79	0.4860822392029114
1105	80-81	0.4861517888606173
1105	82-83	0.47616204808150187
1105	84-85	0.45802923621717184
1105	86-87	0.5114315802876881
1105	88-89	0.5433407213463859
1105	90-91	0.5403708766243156
1105	92-93	0.5424150765320093
1105	94-95	0.6374864099681687
1105	96-97	0.5946878229023618
1105	98-99	0.6627541477161074
1105	100	0.7359688341381627
1106	1	0.2927433663374259
1106	2	0.3113713093197319
1106	3	0.34365174026854817
1106	4	0.3473808687577744
1106	5	0.3090529476759585
1106	6	0.3232440741845295
1106	7	0.38690922816751083
1106	8	0.36493752622730113
1106	9	0.40872359309209827
1106	10-11	0.4631089151139136
1106	12-13	0.44520186318113275
1106	14-15	0.5263082084344433
1106	16-17	0.5048353555149063
1106	18-19	0.5144681034589951
1106	20-21	0.5146853649560299
1106	22-23	0.519565655844012
1106	24-25	0.508109813792295
1106	26-27	0.4915595917019928
1106	28-29	0.5574413925869877
1106	30-31	0.5559437281391126
1106	32-33	0.5621660980226437
1106	34-35	0.4837178230000063
1106	36-37	0.5351448383419921
1106	38-39	0.5554529170413574
1106	40-41	0.5920317716872532
1106	42-43	0.5822061625763624
1106	44-45	0.5375068359081965
1106	46-47	0.6118517941970083
1106	48-49	0.5296583769079533
1106	50-51	0.5172063359536665
1106	52-53	0.6024850754253919
1106	54-55	0.5900857361111065
1106	56-57	0.6375497415281473
1106	58-59	0.6342004591284436
1106	60-61	0.6249938053564179
1106	62-63	0.5450744080902368
1106	64-65	0.5243030520940763
1106	66-67	0.5286520416661489
1106	68-69	0.44382903623600356
1106	70-71	0.39644013352464924
1106	72-73	0.4465735011331802
1106	74-75	0.45300139705041786
1106	76-77	0.5023008796453006
1106	78-79	0.5328474434263022
1106	80-81	0.575961995516888
1106	82-83	0.549598575183257
1106	84-85	0.4999058675188124
1106	86-87	0.4777095227638135
1106	88-89	0.4467464677367694
1106	90-91	0.4972821550773041
1106	92-93	0.5386228571183054
1106	94-95	0.45932628521098096
1106	96-97	0.415290148009575
1106	98-99	0.5463358240303506
1106	100	0.5779701915021249
1107	1	0.2518919852642654
1107	2	0.2654519891564604
1107	3	0.2915215950940464
1107	4	0.32054818930412665
1107	5	0.29977301616820284
1107	6	0.29857402748827155
1107	7	0.29947358412903924
1107	8	0.29379979208552953
1107	9	0.31950133839605144
1107	10-11	0.34198150104294456
1107	12-13	0.37853362786371036
1107	14-15	0.4088275358630469
1107	16-17	0.3799945529271227
1107	18-19	0.4084628336180387
1107	20-21	0.4366793347322542
1107	22-23	0.4061855189255681
1107	24-25	0.3952369083538656
1107	26-27	0.44948881170278554
1107	28-29	0.5074402780193381
1107	30-31	0.46108070850290517
1107	32-33	0.3853142232373017
1107	34-35	0.42185915348004954
1107	36-37	0.39870477153398554
1107	38-39	0.41123770888966504
1107	40-41	0.47976048509640634
1107	42-43	0.41285029185960553
1107	44-45	0.34121688072207945
1107	46-47	0.3017022935233129
1107	48-49	0.32127424818612127
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2316	80-81	-0.5193902009070364
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2316	84-85	-0.6220967059374942
2316	86-87	-0.6386872751376522
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2316	90-91	-0.5420409647318074
2316	92-93	-0.5296358727490862
2316	94-95	-0.3743521512900365
2316	96-97	-0.6332441672904459
2316	98-99	-0.4063831300680576
2316	100	-0.5005909672797486
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	21164.0
3	3359.0
4	3290.0
5	3883.0
6	4654.0
7	5591.0
8	7113.0
9	8560.0
10	11377.0
11	16695.0
12	26166.0
13	38068.0
14	41808.0
15	54259.0
16	73380.0
17	102941.0
18	104593.0
19	58089.0
20	46932.0
21	39941.0
22	40264.0
23	45520.0
24	50196.0
25	53903.0
26	58578.0
27	66747.0
28	78431.0
29	92725.0
30	112213.0
31	137938.0
32	174455.0
33	229649.0
34	330847.0
35	531521.0
36	978109.0
37	1755569.0
38	1591786.0
39	345306.0
40	505.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.02008690929731	17.95107257801032	13.620962760126362	23.40787775256601
2	15.854433848814024	21.524198113973842	37.23675065735972	25.38461737985242
3	18.54113323308024	26.730195179523797	29.711889547762087	25.016782039633874
4	13.231931750131686	17.705682000331507	35.10270002809884	33.95968622143797
5	14.01449731787601	36.36884469684037	32.7718294901711	16.844828495112523
6	30.919232932123396	35.263989121678044	17.623860637045095	16.19291730915347
7	26.16701558184732	32.61942787002625	21.438985304647275	19.77457124347915
8	27.60171797261562	30.680156712783578	20.56243125137361	21.155694063227195
9	27.871002538798106	14.191693170453037	19.54493967285352	38.39236461789534
10-11	25.066382807569127	25.400118262496353	26.224838180394784	23.308660749539733
12-13	27.18494442428248	23.72166502198268	26.54229294124558	22.551097612489254
14-15	25.092818324667338	23.438884583332225	25.637070686058923	25.83122640594152
16-17	22.614588317037118	26.53949152211909	26.61022744982119	24.2356927110226
18-19	23.480328935772565	25.074189874121146	29.384501726261643	22.060979463844646
20-21	24.808260792195806	24.229498158129097	28.430514824214875	22.531726225460222
22-23	23.656524895319937	24.843631886992917	27.614605007093214	23.88523821059393
24-25	22.999330775052236	25.630599422460165	27.4945883345901	23.87548146789749
26-27	23.44889221687562	25.03835368315384	27.6969308869903	23.815823212980238
28-29	22.83502601786187	24.61649235017559	29.235249495878346	23.313232136084196
30-31	23.782137112477855	24.616873421801287	28.497651387142962	23.10333807857789
32-33	22.43205990168968	25.326883004853435	28.215334458718498	24.025722634738383
34-35	22.55110815716148	25.68626669052549	28.490476068747494	23.27214908356553
36-37	23.06551864110091	25.708042824993584	27.650647681505724	23.57579085239978
38-39	23.37076108207368	24.932601909395082	27.666160662819838	24.030476345711406
40-41	22.985691185692986	25.58101537227964	27.69822304263918	23.735070399388196
42-43	24.02715845480408	25.31929216069207	27.307533001167084	23.34601638333677
44-45	23.62329955004537	25.3691927547613	27.294534828102172	23.712972867091153
46-47	23.082731242626963	25.476823989227942	27.73998302185709	23.700461746288003
48-49	23.67138084561122	26.044689417876178	26.857424562428733	23.42650517408387
50-51	22.997670034642876	26.440417406141968	25.97342847728009	24.588484081935057
52-53	22.942273609575803	26.307159672269297	25.431566661309272	25.319000056845624
54-55	23.383824160744464	26.00227201229906	25.497479545962676	25.116424280993805
56-57	23.59453002892544	25.255515988042742	25.668220555699612	25.481733427332205
58-59	23.348548381358	25.07973892989722	26.672159134052333	24.899553554692446
60-61	23.314027196684084	25.38540493140588	26.332323777213652	24.96824409469638
62-63	22.37917609520227	25.48744895742588	26.56628768037474	25.567087266997113
64-65	22.454249558517738	25.40965772049667	26.637957968639853	25.49813475234574
66-67	23.39636142100432	25.293684720544128	26.16445908217094	25.145494776280614
68-69	23.54934343502823	24.801457938558443	26.112080491595712	25.537118134817614
70-71	22.87537110249943	25.176528622659305	26.332154124564767	25.615946150276503
72-73	23.763546802059302	25.015288797910895	26.242182875632864	24.97898152439694
74-75	24.024863225798004	24.773581956498493	26.270228342538903	24.931326475164596
76-77	23.380391493217076	25.339753059719044	26.67968775834879	24.60016768871509
78-79	23.436708021634292	25.34702463020322	26.57631827484367	24.639949073318817
80-81	23.122355779745778	25.19576104418245	26.432030040290737	25.24985313578103
82-83	23.325511971563905	25.460522889752333	25.673413250092086	25.540551888591672
84-85	23.519816396536573	25.42520006627398	25.466416752861477	25.588566784327977
86-87	23.70128499640524	24.735503991673983	25.800757330730022	25.762453681190756
88-89	23.928302166201885	24.333686914904657	26.11126269801693	25.626748220876532
90-91	24.382964872266133	24.811367170060276	25.53544150827718	25.27022644939641
92-93	24.143176339831662	24.831828698032567	25.57521096844413	25.44978399369165
94-95	23.96548128323433	24.916127950721194	25.818682807662746	25.299707958381727
96-97	24.914161744398942	24.69752184945833	25.11567038820301	25.27264601793972
98-99	24.98970557148482	24.430064556403934	25.024370030524818	25.555859841586432
100	25.103120144984885	24.649645837714612	25.118776521834015	25.12845749546649
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3142.0
1	3777.5
2	2874.0
3	1190.5
4	981.5
5	1045.0
6	1414.5
7	2377.5
8	3789.0
9	4101.5
10	3208.0
11	2821.0
12	3127.5
13	3468.0
14	3975.5
15	4525.5
16	5173.5
17	6040.5
18	7085.0
19	8322.5
20	9897.0
21	11939.5
22	14501.0
23	17763.5
24	21566.0
25	26220.0
26	32217.5
27	38663.5
28	45288.0
29	53781.5
30	62507.0
31	70411.5
32	77658.5
33	84413.0
34	91317.5
35	96746.5
36	102348.5
37	109496.5
38	115464.0
39	122527.0
40	128839.5
41	134567.5
42	143686.5
43	161339.0
44	184926.0
45	206614.5
46	234876.5
47	268103.5
48	332647.5
49	401184.5
50	455744.0
51	435481.0
52	361260.0
53	332819.0
54	306128.5
55	277699.0
56	255850.5
57	230017.0
58	212519.0
59	193554.5
60	163107.0
61	133349.0
62	108258.0
63	84821.5
64	63341.0
65	50533.0
66	40231.0
67	30115.0
68	23526.0
69	19464.5
70	16928.5
71	14909.5
72	12669.5
73	12382.5
74	10354.5
75	7978.5
76	6097.5
77	4209.0
78	3063.5
79	2058.0
80	1388.0
81	1020.0
82	772.5
83	562.0
84	402.5
85	269.0
86	177.5
87	132.5
88	103.5
89	85.0
90	66.5
91	60.0
92	54.0
93	40.0
94	36.5
95	36.0
96	28.5
97	19.5
98	11.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08874066259422485
2	0.28570164542530924
3	0.2903571610883289
4	0.299191750752948
5	0.3259269342680665
6	0.17207711548605556
7	0.18887508720583981
8	0.15563307185761202
9	0.20550970749885142
10-11	0.22688151916826896
12-13	0.2446119552825469
14-15	0.17122632680494818
16-17	0.16068335346866547
18-19	0.17808708672939816
20-21	0.20962752471541118
22-23	0.17299596726165156
24-25	0.19751229389644198
26-27	0.19554527046572173
28-29	0.22522077966274737
30-31	0.2605523320117749
32-33	0.22845377665095543
34-35	0.15399275128043696
36-37	0.14868382991032686
38-39	0.11659888716840511
40-41	0.10687267096598546
42-43	0.12300362435978152
44-45	0.1368340451598632
46-47	0.20329765692797225
48-49	0.20775578961697494
50-51	0.21451445489969204
52-53	0.14249689462131396
54-55	0.08679405809185115
56-57	0.12465755755585428
58-59	0.144620463169358
60-61	0.12124759652197586
62-63	0.14238118736068334
64-65	0.11744286954006364
66-67	0.1214245605676462
68-69	0.10643026085180962
70-71	0.132158110568497
72-73	0.1566335993465943
74-75	0.19510966666099475
76-77	0.17552791437662713
78-79	0.20148037230512686
80-81	0.10500774217699808
82-83	0.14509690483077814
84-85	0.17601116234749614
86-87	0.18956252446017458
88-89	0.17149177287345369
90-91	0.174663513076622
92-93	0.12505232350388812
94-95	0.36470247919821674
96-97	0.3653082407391652
98-99	0.41211523081896917
100	0.44664363865303136
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7346125.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.19716395245089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.25711541766957	33.80485445142945
2	12.348043979364254	10.668009605373436
3	3.7566256226603625	4.868267187901128
4	1.6778571469615997	2.899146810643669
5	0.920943149926823	1.9891066119137768
6	0.5828457246077159	1.5106369394918728
7	0.4040850589157939	1.221872997850493
8	0.28281038177354856	0.9773285143141766
9	0.21533121809302175	0.8371528138840699
>10	1.2942823045596101	10.465318275715852
>50	0.12451524950578552	3.7605406395870262
>100	0.11014116213215308	9.699373860645405
>500	0.01569608770725891	4.63096320817577
>1k	0.008856514320105523	7.1129239406942375
>5k	3.7821413453607025E-4	1.039423049819173
>10k+	4.7276766817008786E-4	4.515081092560513
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	44076	0.5999897905358267	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	42853	0.5833415576239174	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37340	0.508295189640797	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24562	0.33435314536575406	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	23628	0.3216389593152853	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	19395	0.26401674352124416	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	18727	0.25492351409756847	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	18302	0.2491381510660382	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17743	0.24152869710221378	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	17045	0.23202708911160647	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	14976	0.20386258061222753	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	14541	0.19794109139172011	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	13874	0.1888614745869421	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	13008	0.17707294662151815	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	11560	0.15736187445762223	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	8720	0.11870203678810258	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	8206	0.11170515067467543	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3141452126120915E-4	0.0	0.0	0.0	0.0
2	3.947659480338274E-4	0.0	0.0	1.3612618897718184E-5	0.0
3	4.0837856693154553E-4	0.0	0.0	1.3612618897718184E-5	0.0
4	4.900542803178546E-4	0.0	0.0	1.3612618897718184E-5	0.0
5	0.002069118072453164	0.0	0.0	1.3612618897718184E-5	0.0
6	0.0035801187700998825	0.0	0.0	1.3612618897718184E-5	0.0
7	0.00397488471813371	0.0	0.0	2.722523779543637E-5	0.0
8	0.004260749714985792	0.0	0.0	2.722523779543637E-5	0.0
9	0.004451326379553846	0.0	0.0	5.445047559087274E-5	0.0
10-11	0.007221494325239497	0.0	0.0	1.2251357007946365E-4	0.0
12-13	0.015028331263080875	0.0	0.0	1.9057666456805458E-4	0.0
14-15	0.02450952032534159	0.0	0.0	2.790586874032228E-4	0.0
16-17	0.02811686433323691	1.3612618897718184E-5	0.0	4.0157225748268643E-4	1.3612618897718184E-5
18-19	0.03050587894978645	2.722523779543637E-5	0.0	6.125678503973184E-4	1.3612618897718184E-5
20-21	0.03991219860810972	5.445047559087274E-5	0.0	0.0012387483196923549	1.3612618897718184E-5
22-23	0.0434923173782096	5.445047559087274E-5	0.0	0.004641903044121902	1.3612618897718184E-5
24-25	0.06532695809014957	5.445047559087274E-5	0.0	0.011836172131565962	1.3612618897718184E-5
26-27	0.1360853511204887	5.445047559087274E-5	0.0	0.022447208562337288	2.722523779543637E-5
28-29	0.15055556500876313	5.445047559087274E-5	0.0	0.06380915108305399	2.722523779543637E-5
30-31	0.15238646225050623	5.445047559087274E-5	0.0	0.1889091187530841	2.722523779543637E-5
32-33	0.1534006023583862	5.445047559087274E-5	0.0	0.3550034882335925	2.722523779543637E-5
34-35	0.1545372560363457	6.125678503973183E-5	0.0	0.5609147679899267	4.0837856693154556E-5
36-37	0.15659276148990114	6.806309448859092E-5	0.0	0.8718950467082986	4.0837856693154556E-5
38-39	0.1585597849206214	6.806309448859092E-5	0.0	1.2800217801902365	5.445047559087274E-5
40-41	0.16026816859228504	6.806309448859092E-5	0.0	1.7812111827664245	5.445047559087274E-5
42-43	0.1634535214143511	8.167571338630911E-5	0.0	2.1692320781364325	5.445047559087274E-5
44-45	0.16743521244193366	8.167571338630911E-5	0.0	2.57217240381834	6.806309448859092E-5
46-47	0.17051847062226683	8.167571338630911E-5	0.0	3.0273171229729963	6.806309448859092E-5
48-49	0.172056696557709	8.167571338630911E-5	0.0	3.452241828174718	8.167571338630911E-5
50-51	0.1743844543892188	1.0890095118174547E-4	0.0	3.9013289319198896	8.167571338630911E-5
52-53	0.17522843676087735	1.2251357007946365E-4	0.0	4.382296789123518	8.167571338630911E-5
54-55	0.17750174411679626	1.2931987952832276E-4	0.0	4.817573890996954	8.167571338630911E-5
56-57	0.1826064762034406	1.3612618897718183E-4	0.0	5.286528612023346	9.528833228402729E-5
58-59	0.1844169545168371	1.3612618897718183E-4	0.0	5.760540420970239	1.0890095118174547E-4
60-61	0.1853153873640865	1.4973880787490004E-4	0.0	6.20152121016182	1.0890095118174547E-4
62-63	0.1860981129507053	1.4973880787490004E-4	0.0	6.667154452177169	1.0890095118174547E-4
64-65	0.18675832496724465	1.4973880787490004E-4	0.0	7.157208732495023	1.2251357007946365E-4
66-67	0.18745937484047712	1.4973880787490004E-4	0.0	7.651455699433376	1.2251357007946365E-4
68-69	0.18825571304599364	1.4973880787490004E-4	0.0	8.191223263965696	1.2251357007946365E-4
70-71	0.18886828089639096	1.5654511732375912E-4	0.0	8.796195273018089	1.4293249842604094E-4
72-73	0.19060388980585002	1.6335142677261822E-4	0.0	9.3407953172591	1.769640456703364E-4
74-75	0.1921897599074342	1.6335142677261822E-4	0.0	9.868107335500008	1.769640456703364E-4
76-77	0.19268662049720092	1.6335142677261822E-4	0.0	10.437611666014394	1.9057666456805458E-4
78-79	0.19312903061137676	1.6335142677261822E-4	0.0	11.017468393200497	1.973829740169137E-4
80-81	0.1940683013153193	1.6335142677261822E-4	0.0	11.642267181677415	2.0418928346577277E-4
82-83	0.194517517738944	1.7015773622147733E-4	0.0	12.322830063468835	2.0418928346577277E-4
84-85	0.1959808742704487	1.769640456703364E-4	0.0	12.968626316595483	2.0418928346577277E-4
86-87	0.19891439364290697	1.9057666456805458E-4	0.0	13.622712654630842	2.0418928346577277E-4
88	0.19956099304054858	2.0418928346577277E-4	0.0	14.134975922680324	2.0418928346577277E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	8615	0.0	23.997545	1
AGTACTT	38015	0.0	22.315027	12-13
GAGTACT	37155	0.0	21.939777	12-13
ATCTACA	9830	0.0	21.155905	2
TCTACAC	11205	0.0	20.488005	3
TACACTC	11145	0.0	20.179102	5
GTACTAG	1735	0.0	20.085833	1
GTACTTT	42115	0.0	19.433796	14-15
TACTTTT	44335	0.0	19.266087	14-15
CTACACT	13190	0.0	18.798738	4
GTATAAG	2260	0.0	18.128773	1
GTATCAA	122670	0.0	17.832201	1
ACTTTTT	46890	0.0	17.633612	16-17
CATGGGG	16380	0.0	17.148388	4
TAGTACT	1910	0.0	16.997248	4
CATGGGT	8910	0.0	16.845188	4
CACTCTT	13775	0.0	16.073473	7
AACGCAG	135620	0.0	16.045044	6
CAACGCA	135750	0.0	15.915343	5
ATGGGTA	5230	0.0	15.743104	5
>>END_MODULE