FastQCFastQC Report
Fri 27 May 2016
ERR765447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765447_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7346125
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA450210.6128537153941704No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT424810.5782776633939662No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338830.46123636611138524No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG255310.347543773077643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243710.3317531351562899No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG208150.28334666235600403No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA200410.2728104953291702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188040.25597168575269275No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC184830.25160203508652523No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG183700.25006380915108306No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT170890.23262604434310608No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC148360.201956813966547No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC142110.19344892715547313No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT131770.17937347921523253No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC130280.17734519899947251No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG89560.12191461484796406No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA83270.11335227756129931No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC19450.029.9596183
GATCTAC35150.024.7478711
CGATCTC22350.023.1284435
TCTCGCG10100.021.8679338
AGTACTT359850.021.28587212-13
GAGTACT348200.020.61473812-13
CCGATCT28550.020.245584
TCTACAC51450.019.9115623
TACTTTT414900.018.64855214-15
TACACTC49550.018.588475
GTACTTT393900.018.27677714-15
ATCTACA49000.018.038852
CTCGCGC13750.017.4300549
GTACTAG16800.017.0730861
ACTTTTT430500.016.91964516-17
GTATAAG21600.016.1090321
CTACACT68500.016.0530134
CATGGGG200300.015.977084
GTATCAA1288100.015.5252021
GTATATA16400.015.1957971
Produced by FastQC (version 0.11.5)