FastQCFastQC Report
Fri 27 May 2016
ERR765446_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765446_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6864256
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA222620.3243177410632703No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT197200.2872853226919276No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG86400.12586943144311635No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG74610.10869349861077442No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG21000.023.6935081
GTACATA19200.022.0396671
CATGGGG156250.017.9152284
TAGTACT25700.017.7312284
GTATAAG24800.017.0629671
TCTAACG10100.017.0343762
TAGGCAT31150.016.8681035
CTAACGC11400.016.7234123
GTACATG572350.016.5498561
TACATGA28650.016.3924052
GTACAAG35350.016.311621
GTATATA18950.016.1956941
TACATGG559500.016.1646162
CTAGGCA35700.016.0207484
CTAGTAC30650.015.7778843
GTATATG24700.015.4376691
TACTAGG30150.015.2684082
ACATGGG526250.015.2420333
GTATTAG26400.015.1481431
ATGGGTA35950.015.1332725
Produced by FastQC (version 0.11.5)