##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765446_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6864256 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.49840142908423 33.0 33.0 33.0 27.0 33.0 2 31.314381048725455 33.0 33.0 33.0 27.0 33.0 3 31.399501562878775 33.0 33.0 33.0 27.0 33.0 4 35.00159507454268 37.0 37.0 37.0 33.0 37.0 5 35.126393304678615 37.0 37.0 37.0 33.0 37.0 6 35.08709159448598 37.0 37.0 37.0 33.0 37.0 7 34.9683206745203 37.0 37.0 37.0 33.0 37.0 8 34.86301414166371 37.0 37.0 37.0 33.0 37.0 9 34.79988843656181 37.0 37.0 37.0 33.0 37.0 10-11 34.809551901910424 37.0 37.0 37.0 33.0 37.0 12-13 34.76365843581591 37.0 37.0 37.0 33.0 37.0 14-15 36.25228844903221 37.0 37.0 40.0 33.0 40.0 16-17 36.310153787970606 37.0 37.0 40.0 33.0 40.0 18-19 36.319380935093335 37.0 37.0 40.0 33.0 40.0 20-21 36.33131034157234 37.0 37.0 40.0 33.0 40.0 22-23 36.38842468870625 37.0 37.0 40.0 33.0 40.0 24-25 36.35998715665616 37.0 37.0 40.0 33.0 40.0 26-27 36.37879516731311 37.0 37.0 40.0 33.0 40.0 28-29 36.30782578621776 37.0 37.0 40.0 33.0 40.0 30-31 36.274380063913696 37.0 37.0 40.0 33.0 40.0 32-33 36.14705213500196 37.0 37.0 40.0 27.0 40.0 34-35 36.093020059275176 37.0 37.0 40.0 27.0 40.0 36-37 35.9797165927378 37.0 37.0 40.0 27.0 40.0 38-39 35.77043979711712 37.0 37.0 40.0 27.0 40.0 40-41 35.6849396351185 37.0 37.0 40.0 27.0 40.0 42-43 35.55238266171891 37.0 35.0 40.0 27.0 40.0 44-45 35.41286280698156 37.0 33.0 40.0 27.0 40.0 46-47 35.293766942841295 37.0 33.0 40.0 27.0 40.0 48-49 35.12849324092808 37.0 33.0 40.0 27.0 40.0 50-51 34.96453213574785 37.0 33.0 40.0 27.0 40.0 52-53 34.78051481471553 37.0 33.0 40.0 27.0 40.0 54-55 34.64773334502676 37.0 33.0 40.0 27.0 40.0 56-57 34.452958339549106 37.0 33.0 40.0 27.0 40.0 58-59 34.22389258209484 37.0 33.0 37.0 27.0 40.0 60-61 33.98784704416619 37.0 33.0 37.0 27.0 40.0 62-63 33.79749655315886 37.0 33.0 37.0 27.0 40.0 64-65 33.49327538192048 37.0 33.0 37.0 24.5 40.0 66-67 33.26374169320025 37.0 33.0 37.0 22.0 40.0 68-69 32.96657511025229 37.0 33.0 37.0 22.0 40.0 70-71 32.8139984143948 37.0 33.0 37.0 22.0 37.0 72-73 32.54412816188673 37.0 33.0 37.0 22.0 37.0 74-75 32.276260894115836 33.0 33.0 37.0 22.0 37.0 76-77 32.09019710220598 33.0 33.0 37.0 22.0 37.0 78-79 31.859897999142223 33.0 33.0 37.0 22.0 37.0 80-81 31.611184373076995 33.0 33.0 37.0 22.0 37.0 82-83 31.44216751240047 33.0 33.0 37.0 22.0 37.0 84-85 31.268013022824324 33.0 27.0 37.0 22.0 37.0 86-87 31.067633986844314 33.0 27.0 37.0 22.0 37.0 88-89 30.89607402462845 33.0 27.0 37.0 18.5 37.0 90-91 30.71074461675089 33.0 27.0 37.0 15.0 37.0 92-93 30.57143345178268 33.0 27.0 37.0 15.0 37.0 94-95 30.344233956309324 33.0 27.0 37.0 15.0 37.0 96-97 30.270442565079158 33.0 27.0 37.0 15.0 37.0 98-99 29.114962495571262 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2883744284493517 1101 2 0.3104929328584234 1101 3 0.3634275068990114 1101 4 0.40387611502619336 1101 5 0.33474343607575463 1101 6 0.3177121913069314 1101 7 0.26109483898089536 1101 8 0.30972112474945845 1101 9 0.2871829301402613 1101 10-11 0.2581058635078648 1101 12-13 0.2825504184067569 1101 14-15 0.4808417380070509 1101 16-17 0.42351852619397334 1101 18-19 0.3520672888545633 1101 20-21 0.33075349128395715 1101 22-23 0.2564860258185604 1101 24-25 0.33990338308731793 1101 26-27 0.3734356038519948 1101 28-29 0.2346844909331196 1101 30-31 0.28991715234933935 1101 32-33 0.4245610957888175 1101 34-35 0.3410745867371645 1101 36-37 0.3749056003286384 1101 38-39 0.3077127090741385 1101 40-41 0.28502200628641106 1101 42-43 0.31594621654196686 1101 44-45 0.2770793526478883 1101 46-47 0.27070284175590587 1101 48-49 0.3154784155005217 1101 50-51 0.31567908383685506 1101 52-53 0.26761783958861685 1101 54-55 0.399035817358218 1101 56-57 0.3830846292420702 1101 58-59 0.29494397826362473 1101 60-61 0.39365562296968903 1101 62-63 0.33921409224584664 1101 64-65 0.2622719344130573 1101 66-67 0.29434386623124453 1101 68-69 0.31664527994109193 1101 70-71 0.36232373919666117 1101 72-73 0.4534924575030601 1101 74-75 0.3542754372694219 1101 76-77 0.35382891220541524 1101 78-79 0.3171822063715801 1101 80-81 0.3116941509067388 1101 82-83 0.32298682817754454 1101 84-85 0.2754610623159621 1101 86-87 0.24154164942287437 1101 88-89 0.30572373336104164 1101 90-91 0.2826472700449081 1101 92-93 0.3302535815603562 1101 94-95 0.32335941613981944 1101 96-97 0.2896235042755002 1101 98-99 0.26706692899859874 1102 1 0.20465474673431316 1102 2 0.2684874129078203 1102 3 0.2344243830586663 1102 4 0.28303143863415414 1102 5 0.19365476478770915 1102 6 0.1590771072643733 1102 7 0.11847574827089602 1102 8 0.10300538129370551 1102 9 0.11646910448522618 1102 10-11 0.09151008145275341 1102 12-13 0.12650720743651078 1102 14-15 0.20024338722441115 1102 16-17 0.2443860724736524 1102 18-19 0.1928475398416012 1102 20-21 0.23752715087661613 1102 22-23 0.17742526149664428 1102 24-25 0.2654739991408803 1102 26-27 0.2797130648268009 1102 28-29 0.14130249080840684 1102 30-31 0.20739956553881456 1102 32-33 0.29800896613271277 1102 34-35 0.25933392974213376 1102 36-37 0.24925163180429877 1102 38-39 0.2908136015890861 1102 40-41 0.21529259336764994 1102 42-43 0.21531687639620145 1102 44-45 0.21781418704176758 1102 46-47 0.2410023479336303 1102 48-49 0.2257884479099843 1102 50-51 0.26433055891610024 1102 52-53 0.18376887552037857 1102 54-55 0.22022277213097396 1102 56-57 0.18307654018372688 1102 58-59 0.1410255471493187 1102 60-61 0.2367165701746572 1102 62-63 0.17332529959341514 1102 64-65 0.1098990457428286 1102 66-67 0.0994863496234828 1102 68-69 0.04291715366737492 1102 70-71 0.16091428494100057 1102 72-73 0.13818547821409766 1102 74-75 0.15923884925134502 1102 76-77 0.07899308872216437 1102 78-79 0.10711361001616027 1102 80-81 0.13272998001715663 1102 82-83 0.08605042927803908 1102 84-85 0.037464854192759134 1102 86-87 0.12452272691966826 1102 88-89 0.09519717843681264 1102 90-91 0.04812295040030534 1102 92-93 0.15113754359063947 1102 94-95 0.11537917511439844 1102 96-97 0.05636049790615161 1102 98-99 0.12588198746034251 1103 1 0.1540835351865013 1103 2 0.15129360266956837 1103 3 0.16393927398785735 1103 4 0.19234319799620891 1103 5 0.17078503209444307 1103 6 0.15326341142984745 1103 7 0.05530646132800854 1103 8 0.1351831778194139 1103 9 0.06405176290798664 1103 10-11 0.11718510039001728 1103 12-13 0.03019922554842225 1103 14-15 0.08606904700909723 1103 16-17 0.06258054170442762 1103 18-19 0.16201361854913188 1103 20-21 0.08957352611134439 1103 22-23 0.09946546736417616 1103 24-25 0.12295414809351257 1103 26-27 0.2038312447623909 1103 28-29 0.16625925890372173 1103 30-31 0.19936182132746438 1103 32-33 0.24911850125675983 1103 34-35 0.14948561516520442 1103 36-37 0.20163549875864817 1103 38-39 0.20655929575527665 1103 40-41 0.16339526026209938 1103 42-43 0.15608619168518345 1103 44-45 0.09077640979857904 1103 46-47 0.06275267145127827 1103 48-49 0.09204998233423822 1103 50-51 0.16040945440038712 1103 52-53 0.05923523061915148 1103 54-55 0.12400817803239761 1103 56-57 0.08656230551931543 1103 58-59 0.16214791835357545 1103 60-61 0.15839717653596352 1103 62-63 0.15029143531448597 1103 64-65 0.08457376160180985 1103 66-67 0.12215445829401972 1103 68-69 0.16448809266520215 1103 70-71 0.07747016045194499 1103 72-73 0.12332917359573514 1103 74-75 0.10488563307190191 1103 76-77 0.03305070550389644 1103 78-79 -0.002224654214881383 1103 80-81 -0.038731094509831365 1103 82-83 0.03198572317016968 1103 84-85 0.01307069218044532 1103 86-87 0.09595643248139041 1103 88-89 0.11373768600005718 1103 90-91 0.0913366903794035 1103 92-93 0.11479196781591838 1103 94-95 0.03841545512628386 1103 96-97 -0.007451676670516605 1103 98-99 0.0503409062492679 1104 1 0.15171334379872192 1104 2 0.06958553226695585 1104 3 0.04107041373108444 1104 4 0.15980577305351318 1104 5 0.13212515788566037 1104 6 0.12823444231481318 1104 7 0.06245704452637568 1104 8 0.07303070951698487 1104 9 0.004926551333888085 1104 10-11 0.03796320546197762 1104 12-13 0.0569127996500427 1104 14-15 0.05682834783460322 1104 16-17 0.060224987540216546 1104 18-19 0.05358000409032826 1104 20-21 0.0230482855342089 1104 22-23 0.049779131179619185 1104 24-25 0.06894637286399075 1104 26-27 0.1213578225771883 1104 28-29 0.06136855914195394 1104 30-31 0.04959702999891391 1104 32-33 0.20912342494174396 1104 34-35 0.1570090919288205 1104 36-37 0.24723269631058287 1104 38-39 0.09227437162294194 1104 40-41 0.11310215781506372 1104 42-43 0.045452258118061195 1104 44-45 0.0029623055327903103 1104 46-47 -0.03173878633898397 1104 48-49 0.08446815789135798 1104 50-51 0.21294514982971435 1104 52-53 0.0813493847722313 1104 54-55 0.2280610230889195 1104 56-57 0.10976079343047473 1104 58-59 0.14197625666417224 1104 60-61 0.18091862734499387 1104 62-63 0.12839027428972116 1104 64-65 0.16375129563487434 1104 66-67 0.11974660226029243 1104 68-69 0.04350623631803785 1104 70-71 0.03252127304705965 1104 72-73 0.05851605767009005 1104 74-75 2.94453580750087E-4 1104 76-77 -0.06687426468704416 1104 78-79 -0.07681857069838216 1104 80-81 -0.06832996111704048 1104 82-83 0.04428416361798426 1104 84-85 3.0930274293083926E-4 1104 86-87 -0.03262877363743755 1104 88-89 -1.941835281726867E-5 1104 90-91 0.03928998022876584 1104 92-93 0.059946819861337985 1104 94-95 0.038062060701257394 1104 96-97 -0.12266784267909614 1104 98-99 -0.019266424491576117 1105 1 0.059639700721923816 1105 2 0.051784595301779035 1105 3 0.1231556225433188 1105 4 0.07831459805205299 1105 5 0.061394073449996256 1105 6 0.02282939609648338 1105 7 0.00941299144871266 1105 8 5.329483666329793E-4 1105 9 -0.05124614085250556 1105 10-11 0.06531390183285168 1105 12-13 0.034227132085597134 1105 14-15 0.05080924650331298 1105 16-17 0.031057827464771037 1105 18-19 0.04631792837458448 1105 20-21 0.06278018600377067 1105 22-23 0.04871415299167836 1105 24-25 0.11420953410241452 1105 26-27 0.11040605817307636 1105 28-29 0.04822703137833173 1105 30-31 0.09483062174531653 1105 32-33 0.09706042688207361 1105 34-35 0.06134891237893925 1105 36-37 0.11197191324410483 1105 38-39 -0.00627122511767908 1105 40-41 -0.041526203419870455 1105 42-43 0.007619793441108413 1105 44-45 8.545239402479865E-4 1105 46-47 -0.049009009313977 1105 48-49 -0.10519648716566365 1105 50-51 0.05332829850787846 1105 52-53 0.04824318998233679 1105 54-55 0.0769260520915438 1105 56-57 0.0789659612807867 1105 58-59 0.034467011760149546 1105 60-61 0.10223638591770623 1105 62-63 0.10355011654652202 1105 64-65 -0.1076804689785078 1105 66-67 -0.02240175166494396 1105 68-69 -0.08570097103309138 1105 70-71 -0.13743106401488348 1105 72-73 -0.027205736199341857 1105 74-75 0.0012709848868794893 1105 76-77 -0.08910070355930344 1105 78-79 -0.08003787556867437 1105 80-81 -0.055618176637437955 1105 82-83 -0.03406709102303651 1105 84-85 -0.1024363296959514 1105 86-87 -0.07339705443867572 1105 88-89 -0.026938843916152422 1105 90-91 -1.0748601611609843E-4 1105 92-93 -0.001736032715502489 1105 94-95 -0.08860664546623198 1105 96-97 -0.1709178636183104 1105 98-99 -0.05939717209662021 1106 1 0.09470355137345976 1106 2 0.14638765874629556 1106 3 0.06830572796918233 1106 4 0.0921491467045854 1106 5 0.04419089592869341 1106 6 0.028193793508719978 1106 7 -0.006579862930422564 1106 8 -0.029207614767720713 1106 9 0.0055403395977506875 1106 10-11 0.007516763873312016 1106 12-13 0.0402304223809935 1106 14-15 -0.005860947660494276 1106 16-17 -0.00652163079215029 1106 18-19 0.06584641037974848 1106 20-21 0.04465692319238457 1106 22-23 0.06325863094035356 1106 24-25 0.056152700822998725 1106 26-27 0.08999054276238638 1106 28-29 -0.025908300385097505 1106 30-31 0.020728998646397656 1106 32-33 0.12411139460927956 1106 34-35 -0.028641141617342214 1106 36-37 -0.018686027435151686 1106 38-39 -0.030653741222437247 1106 40-41 0.09446461376376192 1106 42-43 0.10130801677608048 1106 44-45 0.006978915398526908 1106 46-47 0.030087497740943547 1106 48-49 -0.02225186111628119 1106 50-51 -0.05143074799946845 1106 52-53 -0.0555418473366629 1106 54-55 -0.020463728660722325 1106 56-57 -0.012408100181801274 1106 58-59 -0.03725914245563189 1106 60-61 0.025332817275334207 1106 62-63 0.06386923423577429 1106 64-65 -0.044084312875092735 1106 66-67 -0.019221949295200602 1106 68-69 -0.027405278211375617 1106 70-71 -0.02953986112567719 1106 72-73 0.10081540512132392 1106 74-75 0.034307260727537425 1106 76-77 -0.06321212762681228 1106 78-79 0.02668246985880174 1106 80-81 -0.0833356129464704 1106 82-83 0.022281770155412772 1106 84-85 -0.11496731057215825 1106 86-87 -0.12285430553341214 1106 88-89 0.044933536692553844 1106 90-91 0.010254069169580049 1106 92-93 -0.08379337170642032 1106 94-95 -0.12914863875749205 1106 96-97 -0.13259434162616301 1106 98-99 0.2198168732032073 1107 1 0.011514924468194465 1107 2 0.1173204764292528 1107 3 0.06095518160915958 1107 4 0.055898866802166935 1107 5 0.0800164614090022 1107 6 0.03359001128668382 1107 7 0.012460029626460312 1107 8 -0.019962371402094448 1107 9 -0.1012100885830094 1107 10-11 -0.030689859310342626 1107 12-13 -0.05303133089010714 1107 14-15 -0.052429663348362965 1107 16-17 -0.06995026414727334 1107 18-19 -0.1190911188135928 1107 20-21 -0.07125099896723697 1107 22-23 -0.10906092969827341 1107 24-25 0.041105097440507166 1107 26-27 0.07740697365645843 1107 28-29 -0.006844693323287743 1107 30-31 -0.04582178498712608 1107 32-33 -0.16243203721979427 1107 34-35 -0.15996621049629312 1107 36-37 -0.1086879094633062 1107 38-39 -0.09655548819712578 1107 40-41 -0.19832749553407325 1107 42-43 -0.18324734245921803 1107 44-45 -0.11720791594349578 1107 46-47 -0.21663975541679292 1107 48-49 -0.1716304469508927 1107 50-51 -0.11739789351702257 1107 52-53 -0.16143112798630455 1107 54-55 -0.08466299324801696 1107 56-57 -0.12285992664825329 1107 58-59 -0.014379334446438463 1107 60-61 0.04302105783344956 1107 62-63 -0.20513693630391572 1107 64-65 -0.2495074528405823 1107 66-67 -0.06377026482545034 1107 68-69 -0.15968785706714073 1107 70-71 -0.13238733752404386 1107 72-73 -0.03971723488804457 1107 74-75 -0.14342877465346504 1107 76-77 -0.2271760401592431 1107 78-79 -0.09317026847632448 1107 80-81 -0.2850151735119013 1107 82-83 -0.17017877332490983 1107 84-85 -0.28157270487025343 1107 86-87 -0.20773787949924483 1107 88-89 -0.14801655229380373 1107 90-91 -0.07937206706356648 1107 92-93 -0.16001598706184694 1107 94-95 -0.12295805324790621 1107 96-97 -0.21856891918421795 1107 98-99 0.10877664460505443 1108 1 0.03161161482177732 1108 2 0.046177024189539395 1108 3 0.05147770915716876 1108 4 0.030988993645813423 1108 5 0.12227634171167523 1108 6 -0.11395231502660863 1108 7 -0.04363792902481123 1108 8 -0.0747875572410095 1108 9 -0.016601172477990644 1108 10-11 -0.1028662317846738 1108 12-13 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-0.20072095952857438 2215 74-75 -0.002852111793657741 2215 76-77 -0.10570921581446413 2215 78-79 0.05799079094186865 2215 80-81 0.17485706001321688 2215 82-83 0.03828836840484584 2215 84-85 0.020856107112113165 2215 86-87 -0.00891353774877146 2215 88-89 -0.12148449919044069 2215 90-91 0.09814396726668306 2215 92-93 0.11763271692183963 2215 94-95 0.09894275653246609 2215 96-97 0.07681640169209913 2215 98-99 0.02929044592615071 2216 1 -0.014566148498136755 2216 2 -0.006194804657077668 2216 3 -0.011549677437049155 2216 4 0.04883324634486286 2216 5 -0.096872303986828 2216 6 -0.011734834558644991 2216 7 0.034707254742983196 2216 8 0.016262478659719193 2216 9 0.01922039133408049 2216 10-11 0.008070888451520375 2216 12-13 -0.03670919557876573 2216 14-15 -0.08561988776522611 2216 16-17 -0.06973658348007916 2216 18-19 -0.1351987273667561 2216 20-21 -0.10241675275606354 2216 22-23 -0.19015569257203424 2216 24-25 -0.09597544402542724 2216 26-27 -0.1784774494451753 2216 28-29 -0.07691368746362315 2216 30-31 -0.12562184235364526 2216 32-33 -0.1338084685961647 2216 34-35 -0.27597622982816006 2216 36-37 -0.2960541533452954 2216 38-39 -0.10086860389909447 2216 40-41 -0.1174124222042181 2216 42-43 -0.1595465097380142 2216 44-45 -0.07685501123705762 2216 46-47 -0.02484610368870932 2216 48-49 0.005290221508801096 2216 50-51 -0.085277764618084 2216 52-53 -0.011600988902607412 2216 54-55 -0.03877605578131238 2216 56-57 0.07173732131118271 2216 58-59 -0.1590982844441058 2216 60-61 -0.0913136001213104 2216 62-63 -5.499153334582729E-4 2216 64-65 0.03979147865050692 2216 66-67 -1.2343506473655452E-4 2216 68-69 -0.0214248529901937 2216 70-71 -0.09636397355196635 2216 72-73 -0.11782244004601239 2216 74-75 -0.0017416248225856634 2216 76-77 -0.07771669851055663 2216 78-79 -0.010094410721702474 2216 80-81 0.046969521835414696 2216 82-83 -0.03626278292557217 2216 84-85 -0.021688336166839406 2216 86-87 -0.07074266902627357 2216 88-89 -0.017000480539330454 2216 90-91 0.11504768808359955 2216 92-93 0.1954109709432892 2216 94-95 0.2083482246106101 2216 96-97 0.1684271373797479 2216 98-99 0.20075889855294804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 198.0 8 1221.0 9 2197.0 10 3361.0 11 6173.0 12 12416.0 13 23455.0 14 34682.0 15 39064.0 16 39725.0 17 40808.0 18 40890.0 19 41725.0 20 44310.0 21 47603.0 22 51876.0 23 58346.0 24 65883.0 25 74020.0 26 82873.0 27 93382.0 28 111357.0 29 138311.0 30 175007.0 31 224741.0 32 297764.0 33 399682.0 34 550977.0 35 800955.0 36 1251125.0 37 1605149.0 38 504559.0 39 416.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.30811824783234 18.27372069022385 12.243148838914165 25.175012223029647 2 16.162887864322077 20.74128645551681 37.38427005053425 25.711555629626865 3 18.39604548029813 26.006931277385558 28.608134293535763 26.98888894878054 4 13.169803107576408 17.30461393048278 35.86226970555877 33.66331325638205 5 14.63893537770153 37.39583721819233 32.90755764353777 15.05766976056837 6 32.19517162530069 36.15303683312511 17.495107991310345 14.15668355026386 7 28.21625533779626 31.744328882838868 21.726972304063253 18.31244347530162 8 27.094196370298544 33.391208020213696 19.859311773919856 19.655283835567904 9 27.165318426352396 15.757060925466648 18.890947540418075 38.18667310776288 10-11 25.54115114587801 25.758254062785536 26.47772752065191 22.222867270684542 12-13 26.378751025602774 23.65624184179611 26.08058324165066 23.884423890950455 14-15 24.501570162884367 24.614211649448972 23.31715192440375 27.56706626326291 16-17 24.41927605987277 26.836005740170947 24.38023322412751 24.36448497582877 18-19 24.863233539075466 26.36314846066347 25.39610265118317 23.37751534907789 20-21 26.05181976227648 25.697599637658065 24.37265218721767 23.877928412847783 22-23 25.30646438594365 25.58420752372872 24.137859951610196 24.971468138717437 24-25 24.764714816488883 26.12621800178687 24.192457485350104 24.916609696374138 26-27 25.009902749824995 25.964820654244342 24.113716637858005 24.911559958072655 28-29 24.602971959900362 25.876652409194666 24.777121219350775 24.743254411554197 30-31 25.073283888121185 25.947460534724176 24.483210463284998 24.49604511386965 32-33 24.391448801282703 26.21482975426026 24.1994832941788 25.194238150278238 34-35 24.739237850117277 26.265135750012732 24.668946157073016 24.32668024279698 36-37 24.933802002722512 26.12931394167117 24.23550345441662 24.701380601189697 38-39 24.968902303957407 25.88257763461422 24.268695165658556 24.87982489576982 40-41 24.704847837842877 26.18394477129058 24.648425117011953 24.46278227385459 42-43 25.152711379062787 25.972159255132677 24.44847628060492 24.42665308519962 44-45 24.7114909467246 26.19318102355157 24.346760959964197 24.748567069759638 46-47 24.290243545695265 26.199117573703546 24.961124701642827 24.549514178958358 48-49 24.864404232687225 26.242356314667987 24.605956270934115 24.287283181710677 50-51 24.10147581908367 26.53412110504037 24.49872207563354 24.865681000242414 52-53 24.154810077013444 26.1930571936711 24.293273735711487 25.358858993603967 54-55 24.506057175023777 25.998309212243832 24.42809533910157 25.067538273630817 56-57 24.658098415909897 25.58983814123483 24.25881260838757 25.493250834467712 58-59 24.44438989454939 25.619659290096408 24.861893262722138 25.074057552632066 60-61 24.548275880153653 25.879432526992 24.567025180879035 25.005266411975313 62-63 24.072645309265855 26.014698461129655 24.485763642847818 25.426892586756672 64-65 24.172328363044734 25.719254934547898 24.615042038059187 25.493374664348185 66-67 24.88120884247775 25.679562341274597 24.40139694744621 25.037831868801447 68-69 24.93408608303653 25.38590489632088 24.407794522815 25.272214497827587 70-71 24.309633848154846 25.56786926361721 24.59200239618103 25.53049449204692 72-73 24.96510911014974 25.601609263990156 24.519722166539243 24.913559459320865 74-75 25.197610636899327 25.341369844015144 24.608617452495945 24.85240206658959 76-77 24.772087462938444 25.59672162576687 24.81197525267123 24.819215658623456 78-79 24.877138906241257 25.56847384479833 24.821757813228412 24.732629435732 80-81 24.55421243030563 25.416541865571446 25.106012945904116 24.92323275821881 82-83 24.80556523532922 25.519961668096297 24.619689300632146 25.054783795942342 84-85 24.825880619837022 25.569835973483507 24.415785192160666 25.188498214518805 86-87 24.7767784301751 25.151626046581015 24.6812036147836 25.39039190846029 88-89 24.860684100359894 24.796350835400077 24.91044185997725 25.43252320426278 90-91 25.13994961726369 25.094824551998062 24.61664454239469 25.148581288343557 92-93 24.88594539597591 25.171955999310047 24.633594667797936 25.308503936916104 94-95 24.38895781276223 25.311468569936785 24.99825181345218 25.301321803848808 96-97 24.9653203493576 25.274195775915114 24.538267512167376 25.22221636255991 98-99 24.76750575736103 25.413081913028883 24.398266906129376 25.421145423480706 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 722.0 1 683.5 2 685.0 3 718.5 4 656.0 5 589.0 6 568.5 7 545.5 8 506.0 9 480.5 10 482.0 11 475.0 12 513.0 13 559.5 14 588.5 15 711.5 16 877.0 17 1038.0 18 1310.0 19 1774.0 20 2417.5 21 3452.0 22 4978.5 23 6923.5 24 9495.5 25 13044.5 26 17623.5 27 22681.5 28 28517.5 29 35673.0 30 42565.0 31 50646.0 32 59775.0 33 68849.5 34 77405.0 35 86206.5 36 94145.0 37 103849.5 38 116614.0 39 122047.0 40 126554.5 41 136404.5 42 150989.5 43 172361.5 44 193334.0 45 215764.5 46 250464.5 47 282065.5 48 304725.0 49 328736.5 50 354397.0 51 343517.5 52 317703.5 53 314710.0 54 303490.5 55 291550.5 56 270149.0 57 248316.0 58 234285.5 59 208120.0 60 175918.0 61 138942.0 62 112936.0 63 91088.5 64 68325.0 65 52844.5 66 38138.0 67 28367.5 68 22297.0 69 18917.5 70 16686.0 71 14621.5 72 13807.5 73 12166.0 74 8938.5 75 6637.5 76 4819.5 77 3229.0 78 2116.5 79 1417.0 80 953.5 81 660.5 82 475.0 83 358.0 84 262.5 85 179.0 86 124.0 87 92.5 88 70.0 89 51.5 90 36.5 91 19.5 92 16.5 93 18.0 94 13.0 95 7.5 96 4.0 97 4.0 98 6.0 99 12.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0023891882820221157 2 0.0 3 1.311139910865795E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.284110615921085E-6 18-19 0.0 20-21 3.132167564846066E-4 22-23 0.0 24-25 0.004348614037704888 26-27 1.456822123184217E-5 28-29 0.00879192151341675 30-31 3.714896414119753E-4 32-33 2.913644246368434E-5 34-35 3.642055307960542E-5 36-37 0.0 38-39 2.185233184776325E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 1.6753454416618493E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.0988774311447595E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6864256.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.07044956007605 #Duplication Level Percentage of deduplicated Percentage of total 1 75.06238338740546 33.08032980933743 2 13.669597629281816 12.048506256555989 3 4.592393815916914 6.071665800731146 4 2.074230701290565 3.6564911798874813 5 1.1429501895981296 2.5185164340181863 6 0.6707935089553468 1.7737302900985816 7 0.4744868296152116 1.463759352403431 8 0.348054403451878 1.227113122519068 9 0.2720903733715674 1.0792030567908526 >10 1.4462977011965492 11.702875880316679 >50 0.1219763539178345 3.7416729680850866 >100 0.10155405470281154 9.225219825596671 >500 0.014452936408584948 4.3911638885950985 >1k 0.008405867172992474 6.64751913129836 >5k 2.657981713931583E-4 0.7575197077353101 >10k+ 6.644954284828958E-5 0.6147132960306134 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 22262 0.3243177410632703 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 19720 0.2872853226919276 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 8640 0.12586943144311635 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 7461 0.10869349861077442 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 2.9136442463684337E-5 0.0 0.0 0.0 4 0.0 4.370466369552651E-5 0.0 1.4568221231842169E-5 0.0 5 0.0 4.370466369552651E-5 0.0 5.8272884927368674E-5 0.0 6 0.0 4.370466369552651E-5 0.0 5.8272884927368674E-5 0.0 7 0.0 4.370466369552651E-5 0.0 5.8272884927368674E-5 0.0 8 1.4568221231842169E-5 4.370466369552651E-5 0.0 5.8272884927368674E-5 0.0 9 1.4568221231842169E-5 4.370466369552651E-5 0.0 7.284110615921085E-5 2.9136442463684337E-5 10-11 1.4568221231842169E-5 4.370466369552651E-5 0.0 7.284110615921085E-5 2.9136442463684337E-5 12-13 1.4568221231842169E-5 5.8272884927368674E-5 0.0 9.46934380069741E-5 4.370466369552651E-5 14-15 1.4568221231842169E-5 5.8272884927368674E-5 0.0 1.1654576985473735E-4 5.8272884927368674E-5 16-17 4.370466369552651E-5 5.8272884927368674E-5 0.0 1.6753454416618493E-4 7.284110615921085E-5 18-19 7.284110615921085E-5 5.8272884927368674E-5 0.0 1.9667098662986927E-4 7.284110615921085E-5 20-21 7.284110615921085E-5 8.740932739105302E-5 0.0 4.5161485818710724E-4 7.284110615921085E-5 22-23 7.284110615921085E-5 9.46934380069741E-5 0.0 0.001886584649523561 9.46934380069741E-5 24-25 9.46934380069741E-5 1.0197754862289518E-4 0.0 0.005193570869151733 1.0197754862289518E-4 26-27 1.2382988047065843E-4 1.0197754862289518E-4 0.0 0.009141558822980962 1.1654576985473735E-4 28-29 1.6025043355026385E-4 1.0197754862289518E-4 0.0 0.023229028754172338 1.1654576985473735E-4 30-31 1.7481865478210604E-4 1.0197754862289518E-4 0.0 0.06176197391239487 1.1654576985473735E-4 32-33 1.893868760139482E-4 1.0197754862289518E-4 0.0 0.12503904283290135 1.1654576985473735E-4 34-35 1.893868760139482E-4 1.0197754862289518E-4 0.0 0.20504771383817855 1.456822123184217E-4 36-37 1.893868760139482E-4 1.0926165923881627E-4 0.0 0.3255851763104406 1.456822123184217E-4 38-39 1.893868760139482E-4 1.1654576985473735E-4 0.0 0.5031135202416693 1.456822123184217E-4 40-41 1.893868760139482E-4 1.1654576985473735E-4 0.0 0.7226129095418352 1.456822123184217E-4 42-43 1.893868760139482E-4 1.1654576985473735E-4 0.0 0.9131579591437149 1.456822123184217E-4 44-45 1.893868760139482E-4 1.1654576985473735E-4 0.0 1.1341797275626084 1.456822123184217E-4 46-47 1.893868760139482E-4 1.1654576985473735E-4 0.0 1.380200563615343 1.456822123184217E-4 48-49 1.893868760139482E-4 1.1654576985473735E-4 0.0 1.631167310776288 1.6025043355026385E-4 50-51 1.9667098662986927E-4 1.1654576985473735E-4 0.0 1.908633652357954 1.6025043355026385E-4 52-53 2.0395509724579035E-4 1.1654576985473735E-4 0.0 2.188518318664106 1.893868760139482E-4 54-55 2.1852331847763254E-4 1.3839810170250061E-4 0.0 2.462524707703209 2.0395509724579035E-4 56-57 2.1852331847763254E-4 1.6025043355026385E-4 0.0 2.767692521957223 2.0395509724579035E-4 58-59 2.1852331847763254E-4 1.893868760139482E-4 0.0 3.1018948011262983 2.0395509724579035E-4 60-61 2.1852331847763254E-4 1.893868760139482E-4 0.0 3.4383114499226135 2.1852331847763254E-4 62-63 2.1852331847763254E-4 1.893868760139482E-4 0.0 3.7786250978984466 2.330915397094747E-4 64-65 2.1852331847763254E-4 2.0395509724579035E-4 0.0 4.138219786674623 2.330915397094747E-4 66-67 2.1852331847763254E-4 2.0395509724579035E-4 0.0 4.525756323773472 2.476597609413169E-4 68-69 2.1852331847763254E-4 2.0395509724579035E-4 0.0 4.945204549480672 2.6222798217315907E-4 70-71 2.1852331847763254E-4 2.1123920786171146E-4 0.0 5.422911091894008 2.6222798217315907E-4 72-73 2.476597609413169E-4 2.1852331847763254E-4 0.0 5.878430233371249 3.0593264586868554E-4 74-75 2.6222798217315907E-4 2.1852331847763254E-4 0.0 6.326614275458258 3.0593264586868554E-4 76-77 2.6222798217315907E-4 2.1852331847763254E-4 0.0 6.807707929307998 3.0593264586868554E-4 78-79 2.6222798217315907E-4 2.2580742909355362E-4 0.0 7.305474912357581 3.0593264586868554E-4 80-81 2.6222798217315907E-4 2.330915397094747E-4 0.0 7.84626913681541 3.0593264586868554E-4 82-83 2.6222798217315907E-4 2.330915397094747E-4 0.0 8.418071820165215 3.0593264586868554E-4 84-85 2.6222798217315907E-4 2.330915397094747E-4 0.0 8.977280276260093 3.0593264586868554E-4 86-87 2.6951209278908015E-4 2.330915397094747E-4 0.0 9.551435727338841 3.132167564846066E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 2100 0.0 23.693508 1 GTACATA 1920 0.0 22.039667 1 CATGGGG 15625 0.0 17.915228 4 TAGTACT 2570 0.0 17.731228 4 GTATAAG 2480 0.0 17.062967 1 TCTAACG 1010 0.0 17.034376 2 TAGGCAT 3115 0.0 16.868103 5 CTAACGC 1140 0.0 16.723412 3 GTACATG 57235 0.0 16.549856 1 TACATGA 2865 0.0 16.392405 2 GTACAAG 3535 0.0 16.31162 1 GTATATA 1895 0.0 16.195694 1 TACATGG 55950 0.0 16.164616 2 CTAGGCA 3570 0.0 16.020748 4 CTAGTAC 3065 0.0 15.777884 3 GTATATG 2470 0.0 15.437669 1 TACTAGG 3015 0.0 15.268408 2 ACATGGG 52625 0.0 15.242033 3 GTATTAG 2640 0.0 15.148143 1 ATGGGTA 3595 0.0 15.133272 5 >>END_MODULE