FastQCFastQC Report
Fri 27 May 2016
ERR765446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765446_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6864256
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT216130.31486296548380477No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA212510.3095892693978779No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174730.2545505295839782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116880.17027336975777127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94650.13788821395938614No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG85580.12467483730210528No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG6550.027.6870522
GTACTAG21050.023.4286731
GTATCAA496000.022.4749641
TAACGCA4850.022.051574
AGTACTT284750.021.68894212-13
CAACGCA584250.018.7989925
ATCAACG589550.018.6773173
TACTTTT332400.018.62876914-15
TCTAACG9550.018.5026382
CTAACGC9300.018.500023
AACGCAG604200.018.4780166
TCAACGC605600.018.1899954
TATCAAC614200.018.1397132
GAGTACT273750.018.0160912-13
CATGGGG128500.017.224924
ACGCAGA641750.017.1577267
CGCAGAG649750.016.9536298
TAGTACT24500.016.5122624
ATCACGC11350.016.3876823
ATATACG27100.016.3003986
Produced by FastQC (version 0.11.5)