FastQCFastQC Report
Fri 27 May 2016
ERR765445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765445_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10887346
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT218420.2006182222921913No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA205830.18905433886274947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147300.1352946806319924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG44750.023.0047051
GTACAAG56650.022.321221
TACATGA39850.020.287282
GTACATG776350.019.6481971
TAGGCAT64300.019.2947355
GTATCAA562300.019.2861291
GTATAAG50100.019.0468831
TACATGG766650.018.7974382
TAGTACT52100.018.402224
CTAGGCA71000.017.9386084
GTACATA29650.017.7565631
TATTCGC9350.017.5871129
CATGGGG265750.017.5788674
CTAACGC20650.017.52553
GTACAAA50750.017.5061781
CGTTAAC12550.017.229781
ACATGGG746900.016.99033
TCTAACG21600.016.7556272
ATACGGG31800.016.7013283
GTATAAA152550.016.3008041
Produced by FastQC (version 0.11.5)