##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765445_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10887346
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.425935301404035	33.0	33.0	33.0	27.0	33.0
2	31.28018784375917	33.0	33.0	33.0	27.0	33.0
3	31.26740144016733	33.0	33.0	33.0	27.0	33.0
4	34.804809730488955	37.0	37.0	37.0	27.0	37.0
5	35.05038528214314	37.0	37.0	37.0	33.0	37.0
6	35.060910804157416	37.0	37.0	37.0	33.0	37.0
7	34.896407811417035	37.0	37.0	37.0	33.0	37.0
8	34.860307920773344	37.0	37.0	37.0	33.0	37.0
9	34.556725027385	37.0	37.0	37.0	27.0	37.0
10-11	34.574743697867234	37.0	37.0	37.0	27.0	37.0
12-13	34.561645831775714	37.0	37.0	37.0	27.0	37.0
14-15	35.75395142213722	37.0	37.0	40.0	27.0	40.0
16-17	36.001594879045825	37.0	37.0	40.0	27.0	40.0
18-19	35.82099462072759	37.0	37.0	40.0	27.0	40.0
20-21	35.79967505395713	37.0	37.0	40.0	27.0	40.0
22-23	35.97052885983416	37.0	37.0	40.0	27.0	40.0
24-25	35.9926607457869	37.0	37.0	40.0	27.0	40.0
26-27	35.678267320612385	37.0	37.0	40.0	27.0	40.0
28-29	35.5538609225793	37.0	33.0	40.0	27.0	40.0
30-31	35.34528056699952	37.0	33.0	40.0	27.0	40.0
32-33	35.28911123059743	37.0	33.0	40.0	27.0	40.0
34-35	35.2270222697065	37.0	33.0	40.0	27.0	40.0
36-37	34.992925364914456	37.0	33.0	40.0	27.0	40.0
38-39	34.85936301647803	37.0	33.0	40.0	24.5	40.0
40-41	34.979688530152345	37.0	33.0	40.0	27.0	40.0
42-43	34.88091928005227	37.0	33.0	40.0	27.0	40.0
44-45	34.806337145893956	37.0	33.0	40.0	27.0	40.0
46-47	34.74234239455603	37.0	33.0	40.0	27.0	40.0
48-49	34.54802970347411	37.0	33.0	40.0	22.0	40.0
50-51	34.37336794476818	37.0	33.0	40.0	22.0	40.0
52-53	34.24695857925339	37.0	33.0	40.0	22.0	40.0
54-55	34.11547713281088	37.0	33.0	40.0	22.0	40.0
56-57	33.91198309486995	37.0	33.0	38.5	22.0	40.0
58-59	33.70354074353841	37.0	33.0	37.0	22.0	40.0
60-61	33.43074170693207	37.0	33.0	37.0	22.0	40.0
62-63	33.199096731196015	37.0	33.0	37.0	22.0	40.0
64-65	32.98035150164236	37.0	33.0	37.0	22.0	40.0
66-67	32.748623815207125	37.0	33.0	37.0	22.0	40.0
68-69	32.53308115678513	37.0	33.0	37.0	22.0	38.5
70-71	32.29823342621792	35.0	33.0	37.0	22.0	37.0
72-73	32.054232133340854	33.0	33.0	37.0	22.0	37.0
74-75	31.826210216888484	33.0	33.0	37.0	22.0	37.0
76-77	31.6266351781233	33.0	33.0	37.0	22.0	37.0
78-79	31.430922421313696	33.0	30.0	37.0	22.0	37.0
80-81	31.282270215349087	33.0	27.0	37.0	22.0	37.0
82-83	31.128798836741296	33.0	27.0	37.0	18.5	37.0
84-85	30.957325366531016	33.0	27.0	37.0	15.0	37.0
86-87	30.751458298468698	33.0	27.0	37.0	15.0	37.0
88-89	30.60561977179746	33.0	27.0	37.0	15.0	37.0
90-91	30.494306968842544	33.0	27.0	37.0	15.0	37.0
92-93	30.388629285778187	33.0	27.0	37.0	15.0	37.0
94-95	30.23633863569689	33.0	27.0	37.0	15.0	37.0
96-97	30.1202580500335	33.0	27.0	37.0	15.0	37.0
98-99	29.977287072533564	33.0	27.0	37.0	15.0	37.0
100	29.884462016730247	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	7.0
7	197.0
8	1121.0
9	2353.0
10	4240.0
11	8965.0
12	18776.0
13	32854.0
14	45526.0
15	50851.0
16	55971.0
17	63082.0
18	72085.0
19	83815.0
20	96181.0
21	111587.0
22	124144.0
23	125530.0
24	126143.0
25	136321.0
26	156205.0
27	186757.0
28	226890.0
29	280253.0
30	345720.0
31	428834.0
32	546045.0
33	709230.0
34	916914.0
35	1212092.0
36	1720597.0
37	2235319.0
38	761997.0
39	744.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49960048649212	18.891397111832077	12.72168634674648	24.887316054929318
2	15.97854914085489	21.068998499623184	37.56374488394106	25.388707475580862
3	17.989657046886702	26.38683188516494	29.257111159230785	26.366399908717575
4	12.686834618501516	17.377446605260698	36.666131671178604	33.26958710505918
5	14.066799974241206	37.52937710478694	33.194311789617906	15.209511131353953
6	31.445066057658437	36.92003055330926	17.303944239659362	14.330959149372937
7	27.38922465159055	32.006895040849024	22.09133153905928	18.51254876850115
8	27.047812136138322	32.9495681516479	19.99542193349458	20.007197778719192
9	27.05893094387095	14.425486308698005	19.57201454370165	38.9435682037294
10-11	25.303166793230623	25.37262962395694	26.754740178013144	22.569463404799293
12-13	26.551779644339796	23.198103647695106	26.39839793776509	23.85171877020001
14-15	24.260304402523694	23.869723982825413	24.582992736240282	27.28697887841062
16-17	23.696892165236918	26.366304519656055	25.322273239868988	24.614530075238044
18-19	24.017941702052358	25.466799726696937	27.473047559224273	23.042211012026428
20-21	25.43099225599381	24.88397585634101	26.461248347080506	23.22378354058467
22-23	24.905837438067266	24.695980978011857	26.15754415531592	24.240637428604963
24-25	24.216782586841827	25.170150213051528	26.40411220522329	24.208954994883356
26-27	24.35760594042841	25.050693590669944	26.435895008257564	24.155805460644082
28-29	23.959637651890965	25.07918548449975	26.910484921298316	24.050691942310966
30-31	24.363760212475356	25.05018484267973	26.712363067925367	23.873691876919548
32-33	24.051880883964742	25.21845143714472	26.25070201437696	24.478965664513577
34-35	24.27969524348634	25.279476536303097	26.683765034970175	23.75706318524039
36-37	24.424362965909268	25.064187100994367	26.27886374967166	24.232586183424708
38-39	24.403089892480708	24.864567579600305	26.457196324775712	24.275146203143276
40-41	24.191747860887784	25.06027679285235	26.54249338716267	24.2054819590972
42-43	24.651894698895997	25.011799147741044	26.110104394484644	24.22620175887831
44-45	24.222893604186144	25.39474401295685	25.89726955302706	24.485092829829945
46-47	24.005474556537575	25.378814754741413	26.04230810377008	24.573402584950934
48-49	24.660739491862437	25.465484258588152	25.519867040210908	24.35390920933851
50-51	23.821448178119983	25.790727856169905	25.365646254596907	25.0221777111132
52-53	23.858223577888058	25.67627580400707	25.090378147551768	25.37512247055311
54-55	23.985464409783617	25.515336795579014	25.16021811008854	25.338980684548833
56-57	24.298272508286225	25.037695137088505	25.034563060639385	25.629469293985878
58-59	23.791082785464887	25.059408417809077	25.697860617270727	25.451648179455304
60-61	23.95798296481071	25.184333261751764	25.372914574405918	25.48476919903161
62-63	23.67048133024153	25.076474522668	25.242783187655764	26.0102609594347
64-65	23.415495698690712	24.8721253784619	25.51685678148015	26.19552214136724
66-67	24.1288681189213	24.823994486787864	25.32895894032874	25.7181784539621
68-69	23.90718568168058	24.439535621626113	25.481424309346036	26.17185438734727
70-71	23.370172299669722	24.548513436179704	25.548041374111648	26.53327289003893
72-73	23.923039649654843	24.577461460788264	25.62040402870477	25.879094860852124
74-75	24.068675347940754	24.449048261733672	25.57506230630029	25.90721408402528
76-77	23.633962847830855	24.601348613232332	25.763791349432534	26.00089718950428
78-79	23.766523253992446	24.67706659993476	25.590654765204246	25.96575538086855
80-81	23.388799439936633	24.509991628349606	25.930852987545766	26.170355944167994
82-83	23.562781680994654	24.482126724346017	25.708388550493066	26.24670304416626
84-85	23.61141409152704	24.593355376938202	25.302076960220017	26.493153571314743
86-87	23.37864271721095	24.24610964635754	25.71127043367973	26.663977202751777
88-89	23.59483912236018	23.788885946558775	25.79840862498913	26.81786630609192
90-91	23.786305279287806	24.011979499275853	25.53877779622497	26.662937425211368
92-93	23.61460478535816	23.995372987548336	25.508713951125927	26.881308275967577
94-95	22.971087768195854	24.0172891598856	26.051701043432047	26.9599220284865
96-97	23.558190272301434	23.913661181356023	25.592991526904648	26.9351570194379
98-99	23.27404495310878	24.02717026885523	25.513479502888792	27.185305275147197
100	23.172617148240228	24.07278099475908	25.509400278386607	27.245201578614086
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	507.0
1	512.5
2	651.0
3	811.5
4	720.0
5	640.0
6	841.5
7	1578.5
8	2586.0
9	2682.0
10	1869.5
11	1359.5
12	1340.5
13	1455.0
14	1689.5
15	2007.0
16	2419.0
17	2978.5
18	3876.5
19	4940.0
20	6354.5
21	8201.0
22	10393.5
23	13056.5
24	16338.5
25	20699.5
26	26781.0
27	33919.5
28	41043.0
29	51926.5
30	64811.5
31	76260.5
32	89503.0
33	103817.5
34	119506.0
35	133204.5
36	146092.0
37	163139.5
38	175818.0
39	190127.0
40	203299.5
41	213146.0
42	228386.5
43	254561.5
44	291844.0
45	335744.0
46	386591.5
47	434660.5
48	478830.5
49	510322.0
50	536610.0
51	544057.0
52	526095.5
53	517462.0
54	502522.5
55	470880.0
56	441231.5
57	405276.5
58	375160.5
59	344015.5
60	292711.5
61	236863.0
62	188743.5
63	148876.5
64	111306.5
65	82908.0
66	63234.5
67	45954.0
68	34350.5
69	27753.0
70	23859.0
71	21271.0
72	18394.5
73	17666.0
74	14207.5
75	10078.0
76	7393.0
77	4948.5
78	3419.5
79	2098.5
80	1286.5
81	899.0
82	681.0
83	520.5
84	369.5
85	231.5
86	149.5
87	85.5
88	51.0
89	40.0
90	31.0
91	20.0
92	14.5
93	12.0
94	4.5
95	2.0
96	3.0
97	3.0
98	1.0
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.049240650568099883
2	0.02523112611650259
3	0.022475633639272602
4	0.0231369518338078
5	0.01600941129270623
6	0.025387270690212287
7	0.04888243654605998
8	0.006006973600361374
9	0.003986279117059383
10-11	0.002098766770156841
12-13	0.001180269277746845
14-15	0.001979362096143541
16-17	0.006420297471945871
18-19	0.0048726291972350285
20-21	0.008509879267178612
22-23	0.018815421132018766
24-25	0.025465342977067137
26-27	0.028294315253689924
28-29	0.044340466446092554
30-31	0.042048815202529614
32-33	0.04021182021770962
34-35	0.04790882920410539
36-37	0.045029339565400055
38-39	0.05507770213236541
40-41	0.05208340030710882
42-43	0.0575300904371001
44-45	0.05792963684629845
46-47	0.06273337873160273
48-49	0.06488266286384212
50-51	0.03371804294637095
52-53	3.214741223434986E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.510984954459976E-4
64-65	1.9747696086814913E-4
66-67	4.133238715844982E-4
68-69	0.0013455988263806442
70-71	5.970233700664974E-5
72-73	2.3421686056454896E-4
74-75	3.673989969639984E-5
76-77	1.2858964893739945E-4
78-79	4.59248746204998E-6
80-81	5.970233700664974E-5
82-83	2.755492477229988E-4
84-85	0.0012583415646016945
86-87	0.0038393195182737836
88-89	0.0025993479035202887
90-91	0.003797987131115333
92-93	0.0037428772815707337
94-95	0.0026911976527612884
96-97	0.0021906165193978405
98-99	0.004096498816148582
100	0.003012671775104787
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.0887346E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.518129060429835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.81040483057517	28.81556828327098
2	12.674690961885272	9.764107645419209
3	4.914102064016903	5.67846052553783
4	2.3170809846442717	3.5699849763998355
5	1.301562718198494	2.506688037990673
6	0.8162721430482778	1.8864765452620325
7	0.5312880652264986	1.4324955585262238
8	0.380551535551423	1.172650652841157
9	0.2812394359043424	0.9749535200141349
>10	1.626995843573483	11.802053947455802
>50	0.16451677058041553	4.422136385711716
>100	0.14551347582408125	11.638101195859871
>500	0.022556356519539772	6.089720538123454
>1k	0.0128248201317013	8.84220299673104
>5k	3.249953850932415E-4	0.8540310894237273
>10k+	7.499893502151727E-5	0.5503681014321021
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	21842	0.2006182222921913	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	20583	0.18905433886274947	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14730	0.1352946806319924	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.836994984819992E-5
2	0.0	0.0	0.0	0.0	1.836994984819992E-5
3	0.0	9.18497492409996E-6	0.0	1.836994984819992E-5	2.755492477229988E-5
4	0.0	9.18497492409996E-6	0.0	6.429482446869972E-5	2.755492477229988E-5
5	0.0	9.18497492409996E-6	0.0	8.266477431689964E-5	2.755492477229988E-5
6	0.0	9.18497492409996E-6	0.0	8.266477431689964E-5	2.755492477229988E-5
7	0.0	9.18497492409996E-6	0.0	1.1021969908919952E-4	2.755492477229988E-5
8	9.18497492409996E-6	9.18497492409996E-6	0.0	1.1021969908919952E-4	2.755492477229988E-5
9	9.18497492409996E-6	9.18497492409996E-6	0.0	1.2858964893739945E-4	2.755492477229988E-5
10-11	9.18497492409996E-6	9.18497492409996E-6	0.0	1.4236711132354938E-4	2.755492477229988E-5
12-13	1.3777462386149941E-5	9.18497492409996E-6	0.0	1.7451452355789923E-4	8.266477431689964E-5
14-15	2.29624373102499E-5	9.18497492409996E-6	0.0	1.791070110199492E-4	8.266477431689964E-5
16-17	4.133238715844982E-5	9.18497492409996E-6	0.0	2.0206944833019912E-4	9.18497492409996E-5
18-19	5.051736208254978E-5	2.755492477229988E-5	0.0	2.6636427279889887E-4	1.148121865512495E-4
20-21	6.429482446869972E-5	2.755492477229988E-5	0.0	4.133238715844982E-4	1.377746238614994E-4
22-23	7.347979939279968E-5	2.755492477229988E-5	0.0	0.0016532954863379928	1.5155208624764932E-4
24-25	9.644223670304957E-5	3.673989969639984E-5	0.0	0.005221658244350827	1.7451452355789923E-4
26-27	1.1481218655124949E-4	3.673989969639984E-5	0.0	0.008583359066571412	1.836994984819992E-4
28-29	1.1940467401329948E-4	3.673989969639984E-5	0.0	0.02230571160317675	1.836994984819992E-4
30-31	1.1940467401329948E-4	3.673989969639984E-5	0.0	0.0685750227833303	1.836994984819992E-4
32-33	1.1940467401329948E-4	3.673989969639984E-5	0.0	0.15423869141294858	1.9288447340609914E-4
34-35	1.1940467401329948E-4	3.673989969639984E-5	0.0	0.2628418349155065	2.1125442325429906E-4
36-37	1.2858964893739945E-4	3.673989969639984E-5	0.0	0.44078235412009503	2.1584691071634905E-4
38-39	1.3318213639944943E-4	3.673989969639984E-5	0.0	0.7107746920140133	2.2043939817839903E-4
40-41	1.377746238614994E-4	3.673989969639984E-5	0.0	1.028937630897374	2.29624373102499E-4
42-43	1.4695959878559936E-4	4.133238715844982E-5	0.0	1.32262720409547	2.525868104127489E-4
44-45	1.4695959878559936E-4	4.59248746204998E-5	0.0	1.6609282004999197	2.755492477229988E-4
46-47	1.4695959878559936E-4	4.59248746204998E-5	0.0	2.0343571335015898	2.8473422264709876E-4
48-49	1.4695959878559936E-4	4.59248746204998E-5	0.0	2.427019403994325	3.0310417249529865E-4
50-51	1.4695959878559936E-4	4.59248746204998E-5	0.0	2.8552504898806377	3.0310417249529865E-4
52-53	1.4695959878559936E-4	4.59248746204998E-5	0.0	3.2703929865001076	3.0769665995734863E-4
54-55	1.4695959878559936E-4	5.510984954459976E-5	0.0	3.6815262415652077	3.168816348814486E-4
56-57	1.561445737096993E-4	6.88873119307497E-5	9.18497492409996E-6	4.143098786426004	3.260666098055486E-4
58-59	1.561445737096993E-4	8.266477431689964E-5	9.18497492409996E-6	4.6350322658984116	3.3065909726759856E-4
60-61	1.607370611717493E-4	8.725726177894963E-5	9.18497492409996E-6	5.129753385260283	3.3065909726759856E-4
62-63	1.6532954863379928E-4	1.0103472416509956E-4	9.18497492409996E-6	5.628483746176524	3.4902904711579845E-4
64-65	1.6532954863379928E-4	1.0103472416509956E-4	9.18497492409996E-6	6.149648408344881	3.5362153457784844E-4
66-67	1.6532954863379928E-4	1.1021969908919952E-4	9.18497492409996E-6	6.710221205425087	3.582140220398984E-4
68-69	1.6532954863379928E-4	1.1940467401329948E-4	9.18497492409996E-6	7.3079426335858155	3.582140220398984E-4
70-71	1.6532954863379928E-4	1.1940467401329948E-4	9.18497492409996E-6	7.958514407459816	3.673989969639984E-4
72-73	1.6532954863379928E-4	1.1940467401329948E-4	9.18497492409996E-6	8.585503758216191	4.0413889666039823E-4
74-75	1.6532954863379928E-4	1.2858964893739945E-4	9.18497492409996E-6	9.205333421019228	4.3169382143269815E-4
76-77	1.6532954863379928E-4	1.2858964893739945E-4	9.18497492409996E-6	9.866725095353818	4.5006377128089804E-4
78-79	1.7451452355789923E-4	1.2858964893739945E-4	9.18497492409996E-6	10.54382766929608	4.5006377128089804E-4
80-81	1.7451452355789923E-4	1.3318213639944943E-4	9.18497492409996E-6	11.264021553094757	4.5006377128089804E-4
82-83	1.7451452355789923E-4	1.377746238614994E-4	9.18497492409996E-6	12.008895464514492	4.6843372112909793E-4
84-85	1.7451452355789923E-4	1.561445737096993E-4	9.18497492409996E-6	12.742223862454633	4.6843372112909793E-4
86-87	1.7451452355789923E-4	1.7451452355789923E-4	9.18497492409996E-6	13.486307866030895	4.776186960531979E-4
88	1.7451452355789923E-4	1.7451452355789923E-4	9.18497492409996E-6	14.066026743340387	4.868036709772979E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	4475	0.0	23.004705	1
GTACAAG	5665	0.0	22.32122	1
TACATGA	3985	0.0	20.28728	2
GTACATG	77635	0.0	19.648197	1
TAGGCAT	6430	0.0	19.294735	5
GTATCAA	56230	0.0	19.286129	1
GTATAAG	5010	0.0	19.046883	1
TACATGG	76665	0.0	18.797438	2
TAGTACT	5210	0.0	18.40222	4
CTAGGCA	7100	0.0	17.938608	4
GTACATA	2965	0.0	17.756563	1
TATTCGC	935	0.0	17.587112	9
CATGGGG	26575	0.0	17.578867	4
CTAACGC	2065	0.0	17.5255	3
GTACAAA	5075	0.0	17.506178	1
CGTTAAC	1255	0.0	17.22978	1
ACATGGG	74690	0.0	16.9903	3
TCTAACG	2160	0.0	16.755627	2
ATACGGG	3180	0.0	16.701328	3
GTATAAA	15255	0.0	16.300804	1
>>END_MODULE