FastQCFastQC Report
Fri 27 May 2016
ERR765444_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765444_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6678019
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA598980.8969426412233926No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT536150.8028578535041605No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG235670.35290405732598246No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG202240.3028443015810527No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC194850.2917781455847909No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA184790.2767137979092303No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT182530.2733295607574641No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG168670.2525749028267215No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC161900.24243716587209468No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC154120.23078700434964322No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC115440.17286563575215944No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT108180.16199414826462757No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG83600.12518682561400318No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA81770.12244649199111292No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC75450.11298260756670503No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA74880.11212906102842773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGA26650.033.5006072
GTACAAG28150.032.871791
GTACATA17100.031.5435491
GTACTAG28850.025.14361
GTGATCG20300.024.9649318
GTATAAG27050.023.3786981
GTACAAA38950.022.9214691
TGATCGC23400.021.6589099
TACAAGA22000.021.558882
CTATTGA36400.021.0769849
TAGGCAT39900.020.8589765
TAAGGTG27550.020.5897544
ATAAGGT27100.019.9038643
CGTTAAC7750.019.7998051
CTAGGCA46950.018.915194
TCTAACG18000.018.5998172
TTTAGGG45350.018.4562682
GTATAAT28650.018.3401341
ATACGGG20050.018.3215163
GGCTATT44000.018.2807227
Produced by FastQC (version 0.11.5)