##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765444_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6678019 Sequences flagged as poor quality 0 Sequence length 99 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.169022879389832 33.0 33.0 33.0 27.0 33.0 2 31.01260613364532 33.0 33.0 33.0 27.0 33.0 3 31.051565891022474 33.0 33.0 33.0 27.0 33.0 4 34.49452210303684 37.0 37.0 37.0 27.0 37.0 5 34.73399776191113 37.0 37.0 37.0 27.0 37.0 6 34.59424344255385 37.0 37.0 37.0 27.0 37.0 7 34.52321085040339 37.0 37.0 37.0 27.0 37.0 8 34.36395164494141 37.0 37.0 37.0 27.0 37.0 9 34.15867340299571 37.0 37.0 37.0 27.0 37.0 10-11 34.303582244974145 37.0 37.0 37.0 27.0 37.0 12-13 34.21865166900544 37.0 37.0 37.0 27.0 37.0 14-15 35.61787365384855 37.0 37.0 40.0 27.0 40.0 16-17 35.73178625277946 37.0 37.0 40.0 27.0 40.0 18-19 35.765482248553056 37.0 37.0 40.0 27.0 40.0 20-21 35.80981942399385 37.0 37.0 40.0 27.0 40.0 22-23 35.897903929294 37.0 37.0 40.0 27.0 40.0 24-25 36.02540004752906 37.0 37.0 40.0 27.0 40.0 26-27 36.00015768149207 37.0 37.0 40.0 27.0 40.0 28-29 36.01074382986931 37.0 37.0 40.0 27.0 40.0 30-31 35.907322440981375 37.0 37.0 40.0 27.0 40.0 32-33 35.77371133565208 37.0 37.0 40.0 27.0 40.0 34-35 35.62671212825241 37.0 35.0 40.0 27.0 40.0 36-37 35.41063764568504 37.0 33.0 40.0 27.0 40.0 38-39 35.07317304727644 37.0 33.0 40.0 27.0 40.0 40-41 35.14514776013665 37.0 33.0 40.0 27.0 40.0 42-43 35.031572237215855 37.0 33.0 40.0 27.0 40.0 44-45 34.91553228584705 37.0 33.0 40.0 27.0 40.0 46-47 34.8072182034822 37.0 33.0 40.0 27.0 40.0 48-49 34.66492390932101 37.0 33.0 40.0 27.0 40.0 50-51 34.48167450556819 37.0 33.0 40.0 27.0 40.0 52-53 34.33956814139043 37.0 33.0 40.0 22.0 40.0 54-55 34.12945268649281 37.0 33.0 40.0 22.0 40.0 56-57 33.89759814999029 37.0 33.0 37.0 22.0 40.0 58-59 33.67958791372112 37.0 33.0 37.0 22.0 40.0 60-61 33.44278325653161 37.0 33.0 37.0 22.0 40.0 62-63 33.17402660878922 37.0 33.0 37.0 22.0 40.0 64-65 32.97580629824503 37.0 33.0 37.0 22.0 40.0 66-67 32.75725158913144 37.0 33.0 37.0 22.0 40.0 68-69 32.53474518416314 37.0 33.0 37.0 22.0 40.0 70-71 32.24381676661896 35.0 33.0 37.0 22.0 37.0 72-73 31.873229546067478 33.0 33.0 37.0 22.0 37.0 74-75 31.618799227735053 33.0 27.0 37.0 22.0 37.0 76-77 31.403577018873413 33.0 27.0 37.0 22.0 37.0 78-79 31.15125076763034 33.0 27.0 37.0 18.5 37.0 80-81 30.950877123889583 33.0 27.0 37.0 15.0 37.0 82-83 30.819753657484352 33.0 27.0 37.0 15.0 37.0 84-85 30.6423213231349 33.0 27.0 37.0 15.0 37.0 86-87 30.339189061905934 33.0 27.0 37.0 15.0 37.0 88-89 30.165834808196863 33.0 27.0 37.0 15.0 37.0 90-91 30.006613338476576 33.0 27.0 37.0 15.0 37.0 92-93 29.71017774882042 33.0 27.0 37.0 15.0 37.0 94-95 29.573876549317994 33.0 27.0 37.0 15.0 37.0 96-97 29.390785950144796 33.0 27.0 37.0 15.0 37.0 98-99 28.31656101906868 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33047456429596167 1101 2 0.3643589240941729 1101 3 0.4218143581644007 1101 4 0.3652180681253654 1101 5 0.36272381539793486 1101 6 0.4844369970602713 1101 7 0.3678357977695299 1101 8 0.4451898677518358 1101 9 0.38901023137066204 1101 10-11 0.3402292497559145 1101 12-13 0.48509356470867004 1101 14-15 0.5676160606913001 1101 16-17 0.6026350931965538 1101 18-19 0.4952702076169757 1101 20-21 0.43833528547590817 1101 22-23 0.4381613096006092 1101 24-25 0.32375172189603774 1101 26-27 0.4377651748242144 1101 28-29 0.43224119107285475 1101 30-31 0.6544774541336835 1101 32-33 0.7019754769005857 1101 34-35 0.7575518454896795 1101 36-37 0.8723785804993867 1101 38-39 0.8985917234454206 1101 40-41 0.6308905522741952 1101 42-43 0.5439714793261601 1101 44-45 0.43815493078665213 1101 46-47 0.4548237931144783 1101 48-49 0.3672362285019162 1101 50-51 0.31335178286607857 1101 52-53 0.44787315719666765 1101 54-55 0.30760607226195447 1101 56-57 0.4227796207527632 1101 58-59 0.3074695072962399 1101 60-61 0.24684756431202004 1101 62-63 0.4466875274044 1101 64-65 0.44919114457636766 1101 66-67 0.4426600913549734 1101 68-69 0.5149023100103491 1101 70-71 0.49581109478348395 1101 72-73 0.30885416240834473 1101 74-75 0.34537181391711513 1101 76-77 0.2514601098375415 1101 78-79 0.27616839485053646 1101 80-81 0.28132806299082347 1101 82-83 0.3944043153189085 1101 84-85 0.4094018233350667 1101 86-87 0.3068282562706557 1101 88-89 0.20338825163776875 1101 90-91 0.0913518851027959 1101 92-93 0.25210625955833876 1101 94-95 0.29207307502220203 1101 96-97 0.19072478569539442 1101 98-99 0.18748784705441324 1102 1 0.307804742270136 1102 2 0.32238872121859075 1102 3 0.31619213309661376 1102 4 0.4148276347104485 1102 5 0.271294706686227 1102 6 0.46837532903658996 1102 7 0.3449079216451594 1102 8 0.3877933169024743 1102 9 0.32534331021356877 1102 10-11 0.3114607679926493 1102 12-13 0.3771473326568895 1102 14-15 0.5472315083407651 1102 16-17 0.357520504049603 1102 18-19 0.43398735237934716 1102 20-21 0.3467905688502029 1102 22-23 0.38206629406943904 1102 24-25 0.3488930506582051 1102 26-27 0.49689649873013053 1102 28-29 0.3972401904261602 1102 30-31 0.5395170612197902 1102 32-33 0.5762410787341992 1102 34-35 0.6516984649950857 1102 36-37 0.735379165585762 1102 38-39 0.7547651598795895 1102 40-41 0.571301538207166 1102 42-43 0.4624737689346574 1102 44-45 0.3902259976411244 1102 46-47 0.372440047321426 1102 48-49 0.23507032973595443 1102 50-51 0.24461196550322484 1102 52-53 0.2885754279599837 1102 54-55 0.20717711294257413 1102 56-57 0.37468756407548653 1102 58-59 0.2559827818712819 1102 60-61 0.2494063105278883 1102 62-63 0.33054850556573 1102 64-65 0.26507254519182055 1102 66-67 0.3135661578108042 1102 68-69 0.4576686813553721 1102 70-71 0.4190156185118994 1102 72-73 0.233564659413652 1102 74-75 0.3278743386876535 1102 76-77 0.33813026769408694 1102 78-79 0.2864831294435959 1102 80-81 0.21530521281078663 1102 82-83 0.20793403512977804 1102 84-85 0.3495767412095532 1102 86-87 0.2650807023182793 1102 88-89 0.21125870030600424 1102 90-91 0.24045819888144138 1102 92-93 0.24084858155798727 1102 94-95 0.2440629123174496 1102 96-97 0.11767150659429149 1102 98-99 0.16919512950737925 1103 1 0.24865341394842844 1103 2 0.28559116533854123 1103 3 0.17781151780013005 1103 4 0.2873563233745955 1103 5 0.16239986874288093 1103 6 0.38057244304410887 1103 7 0.27306662919805547 1103 8 0.24049808355731983 1103 9 0.29398553367768443 1103 10-11 0.22655636849662386 1103 12-13 0.2435071072337962 1103 14-15 0.42104367545911003 1103 16-17 0.20520437890712628 1103 18-19 0.22367177707373287 1103 20-21 0.2831202626441538 1103 22-23 0.24323586916273854 1103 24-25 0.12415211996168551 1103 26-27 0.3189824886067214 1103 28-29 0.3303985824988942 1103 30-31 0.4611250986527651 1103 32-33 0.5244910652136241 1103 34-35 0.5736574365498015 1103 36-37 0.7220155010884 1103 38-39 0.6567961260100432 1103 40-41 0.45156249538398185 1103 42-43 0.369117255320937 1103 44-45 0.2785207572652766 1103 46-47 0.22221480450185283 1103 48-49 0.21075522214218978 1103 50-51 0.10851798045798233 1103 52-53 0.2581176462161636 1103 54-55 0.17766251672713906 1103 56-57 0.2059004450803883 1103 58-59 0.18969416559730945 1103 60-61 0.12730114146056337 1103 62-63 0.18925670945205297 1103 64-65 0.2736581875394748 1103 66-67 0.31721861685346653 1103 68-69 0.32628346305311595 1103 70-71 0.36346204096687273 1103 72-73 0.18037510491170394 1103 74-75 0.29099127766878397 1103 76-77 0.27470548517509386 1103 78-79 0.2989801999849604 1103 80-81 0.2026271668247972 1103 82-83 0.19734704673344083 1103 84-85 0.3338191080919479 1103 86-87 0.2506608302730271 1103 88-89 0.19880997835118563 1103 90-91 0.13029545618705995 1103 92-93 0.09215021264049739 1103 94-95 0.18448157952894917 1103 96-97 0.009864849862744052 1103 98-99 0.03839723010448637 1104 1 0.13106318988750232 1104 2 0.12025387226681516 1104 3 0.09728339738921221 1104 4 0.1585599236893529 1104 5 0.113589674755886 1104 6 0.2253748315237587 1104 7 0.18526993749110687 1104 8 0.18569843534300645 1104 9 0.12930596427023744 1104 10-11 0.10920410563829108 1104 12-13 0.21774286462724035 1104 14-15 0.2318563515806531 1104 16-17 0.07851411956566778 1104 18-19 0.20455028854480872 1104 20-21 0.16411966170526426 1104 22-23 0.09884114138925071 1104 24-25 -6.110110428991788E-4 1104 26-27 0.1139741713365865 1104 28-29 0.02298593482973388 1104 30-31 0.23760545926808163 1104 32-33 0.35483394528747425 1104 34-35 0.38622884615488573 1104 36-37 0.45436350858759056 1104 38-39 0.47500873321005344 1104 40-41 0.24857177719552936 1104 42-43 0.1543689343457828 1104 44-45 0.1571764796660986 1104 46-47 0.15996903259330253 1104 48-49 -0.04103285347473218 1104 50-51 -0.0437736251625509 1104 52-53 0.07080925859488474 1104 54-55 -0.06972275428171315 1104 56-57 0.05087471247929187 1104 58-59 0.0662514605865212 1104 60-61 -0.06974624013642483 1104 62-63 0.01803544387335876 1104 64-65 0.0588351266021121 1104 66-67 0.061290591157153074 1104 68-69 0.16289967624636148 1104 70-71 0.18837820594304588 1104 72-73 -0.0341227932018171 1104 74-75 -0.028467890328276013 1104 76-77 0.013463514147527889 1104 78-79 0.04614488013421436 1104 80-81 -0.02888951355319591 1104 82-83 0.06410736329257105 1104 84-85 0.11291283806293961 1104 86-87 -0.06466697034362667 1104 88-89 -0.07228260672675191 1104 90-91 -0.22810860568394986 1104 92-93 -0.05843400573056101 1104 94-95 -0.02780901536256053 1104 96-97 -0.12009683740889798 1104 98-99 -0.12639713852528445 1105 1 0.009698458174081992 1105 2 -0.02442093639688281 1105 3 0.03063875099981317 1105 4 -0.11272806033379368 1105 5 -0.11073251009449336 1105 6 0.08186876881575245 1105 7 -0.03598693424510202 1105 8 0.048175485145456776 1105 9 -0.06247297808677388 1105 10-11 -0.04521601781907236 1105 12-13 -0.017801951887854273 1105 14-15 0.013294269808433512 1105 16-17 -0.1469207515212787 1105 18-19 -0.033671753045439345 1105 20-21 -0.07970265031178059 1105 22-23 -0.07126499109632078 1105 24-25 -0.14255926501751048 1105 26-27 -0.038190000283172765 1105 28-29 -0.0601840440577206 1105 30-31 0.08772744169021962 1105 32-33 0.15794036298453307 1105 34-35 0.2173853774104586 1105 36-37 0.3101750014560025 1105 38-39 0.26185055855486183 1105 40-41 0.08708449957285325 1105 42-43 -0.09260614296763947 1105 44-45 -0.06857571049332734 1105 46-47 -0.07217216301547325 1105 48-49 -0.18296753947888078 1105 50-51 -0.20816371863577388 1105 52-53 -0.14712071249543612 1105 54-55 -0.22391854089885754 1105 56-57 0.011082211125284402 1105 58-59 -0.17714758463196034 1105 60-61 -0.25038821027548863 1105 62-63 -0.2435526364226206 1105 64-65 -0.13316931346479777 1105 66-67 -0.09114396023509386 1105 68-69 0.013614285086866573 1105 70-71 0.06546754359312956 1105 72-73 -0.1538141925055001 1105 74-75 -0.08172170908499865 1105 76-77 -0.1547497346141533 1105 78-79 -0.19990638982684317 1105 80-81 -0.21757843635450058 1105 82-83 -0.14990277704609056 1105 84-85 -0.1000917736167466 1105 86-87 -0.2154818489349779 1105 88-89 -0.234354840211207 1105 90-91 -0.2591783078063621 1105 92-93 -0.26551266181220967 1105 94-95 -0.22699157306155726 1105 96-97 -0.2914665936797505 1105 98-99 -0.2688163137333568 1106 1 0.18986966924706294 1106 2 0.15809632878714908 1106 3 0.164041770172215 1106 4 0.044180536515725066 1106 5 0.038711122393848996 1106 6 0.176737500362961 1106 7 0.15468941083343424 1106 8 0.1237565110404617 1106 9 0.14842418120110068 1106 10-11 0.19258130240164206 1106 12-13 0.1771987427455599 1106 14-15 0.28525338423992963 1106 16-17 0.11830493106802464 1106 18-19 0.2731139004987213 1106 20-21 0.1619110873601599 1106 22-23 0.1601155822195537 1106 24-25 0.18731488405655483 1106 26-27 0.25720154097113124 1106 28-29 0.15836137630751068 1106 30-31 0.3768102672161291 1106 32-33 0.36445712635993743 1106 34-35 0.4110702245986815 1106 36-37 0.5811625785564232 1106 38-39 0.5379292816676937 1106 40-41 0.36843924783196513 1106 42-43 0.2757948180068155 1106 44-45 0.19184594378607756 1106 46-47 0.2059581454564352 1106 48-49 0.10332070762870416 1106 50-51 0.05446503909555389 1106 52-53 0.11373625050588743 1106 54-55 0.017351802543132067 1106 56-57 0.04635141480456184 1106 58-59 0.021202834113161373 1106 60-61 0.0015016376096639306 1106 62-63 0.11107869161538275 1106 64-65 0.22464355645531242 1106 66-67 0.13865478519730345 1106 68-69 0.1560601068932499 1106 70-71 0.19716782378463904 1106 72-73 -0.0011740187334048358 1106 74-75 0.16983737034533064 1106 76-77 0.18716893878352536 1106 78-79 0.1593478899848506 1106 80-81 0.16148613056472527 1106 82-83 0.1470606995835091 1106 84-85 0.1423787665130476 1106 86-87 0.08809732766039957 1106 88-89 0.06689817278401122 1106 90-91 -0.008080244192669284 1106 92-93 0.01825802108234953 1106 94-95 0.039824832516739406 1106 96-97 -0.09302170459831416 1106 98-99 -0.11174427231690132 1107 1 0.041319064610405576 1107 2 0.10704815447322602 1107 3 -0.0033121798983231088 1107 4 -0.07016534091790305 1107 5 4.041178071787499E-4 1107 6 0.02142063239107017 1107 7 0.03192812785734844 1107 8 0.045576535878424806 1107 9 -0.1033148964658821 1107 10-11 -0.011876976707561937 1107 12-13 0.004305866730476282 1107 14-15 0.07096258711209913 1107 16-17 -0.03921174135150807 1107 18-19 0.2097858558961434 1107 20-21 0.05448782765449067 1107 22-23 -0.07027411282150808 1107 24-25 -0.018596059260147513 1107 26-27 -0.009302855089160289 1107 28-29 0.005834094132318057 1107 30-31 0.21167272867343456 1107 32-33 0.2248851598172763 1107 34-35 0.2331689949124538 1107 36-37 0.36097181812376533 1107 38-39 0.30365401802724534 1107 40-41 0.1518687370045626 1107 42-43 -0.009846210407232547 1107 44-45 0.008865867175337883 1107 46-47 -0.05602026267316518 1107 48-49 -0.17165135605176118 1107 50-51 -0.11566851750302476 1107 52-53 -0.03316142862601623 1107 54-55 -0.11481420419627852 1107 56-57 -0.04906484142595957 1107 58-59 -0.042382822070976545 1107 60-61 -0.1361492680835923 1107 62-63 -0.08926936800172314 1107 64-65 0.02027984100855207 1107 66-67 -3.1174108743670104E-4 1107 68-69 0.10378053025606704 1107 70-71 0.16090112432814152 1107 72-73 -0.23293123970591978 1107 74-75 0.0023611906624694257 1107 76-77 -0.07935307824315174 1107 78-79 -0.05109752786543709 1107 80-81 -0.04609344410476268 1107 82-83 -0.005037727511012946 1107 84-85 0.11076020949689536 1107 86-87 -0.1038723050965018 1107 88-89 -0.24716881647870892 1107 90-91 -0.18177117067735793 1107 92-93 -0.14853562544117693 1107 94-95 -0.06091401638748195 1107 96-97 -0.1854270943722156 1107 98-99 -0.2292530146444136 1108 1 0.09812893875133 1108 2 0.045777308023126295 1108 3 0.017133720581075096 1108 4 -0.003953415284968287 1108 5 0.052335475327829784 1108 6 0.04899656484590764 1108 7 0.06322866978620567 1108 8 -0.036648301591554855 1108 9 0.043769319670154516 1108 10-11 -0.00995195281735306 1108 12-13 -0.05775514985884911 1108 14-15 -0.064350075742162 1108 16-17 -0.053501571339040765 1108 18-19 0.17474111224200328 1108 20-21 0.16857073878264117 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2215 98-99 0.23655708815291376 2216 1 -0.31035497565745374 2216 2 -0.23796143913692447 2216 3 -0.1506871795609328 2216 4 -0.1609396938763865 2216 5 -0.23235932606775833 2216 6 -0.2051859135314018 2216 7 -0.3084379863712172 2216 8 -0.29270075403720597 2216 9 -0.1925955595805391 2216 10-11 -0.3191520017417062 2216 12-13 -0.42619641957510623 2216 14-15 -0.4188688693352418 2216 16-17 -0.44758064796673125 2216 18-19 -0.3395626322035241 2216 20-21 -0.19276419852542404 2216 22-23 -0.20384016753494194 2216 24-25 -0.3772476205877311 2216 26-27 -0.4826216116007416 2216 28-29 -0.42696802698839775 2216 30-31 -0.31092755297755303 2216 32-33 -0.17369363801751092 2216 34-35 -0.2533947171516999 2216 36-37 -0.13961423640748905 2216 38-39 -8.561936703414936 2216 40-41 -1.9972804521523528 2216 42-43 -0.6331692601732257 2216 44-45 -0.6477437455984187 2216 46-47 -0.3701129588436842 2216 48-49 -0.27346056635611404 2216 50-51 -0.343134076999263 2216 52-53 -0.32092524447054416 2216 54-55 -0.368604435110953 2216 56-57 -0.24726248008542484 2216 58-59 -0.3377090663893725 2216 60-61 -0.3624491752683241 2216 62-63 -0.4284693364897407 2216 64-65 -0.45876100125205 2216 66-67 -0.3235604559788996 2216 68-69 -0.336250482391911 2216 70-71 -0.1922838926694368 2216 72-73 -0.21809960613020607 2216 74-75 -0.18882752460687513 2216 76-77 -0.3255873585216449 2216 78-79 -0.33413021952171107 2216 80-81 -0.2883299622737461 2216 82-83 -0.46151691054096844 2216 84-85 -0.32021592894933804 2216 86-87 -0.2515709899942351 2216 88-89 -0.19915066790544955 2216 90-91 -0.20328743511079495 2216 92-93 -0.0877723522842544 2216 94-95 -0.007516297733470623 2216 96-97 -0.03759611988274969 2216 98-99 0.008028101075410632 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 250.0 8 1358.0 9 2371.0 10 3696.0 11 7221.0 12 16260.0 13 32789.0 14 50199.0 15 54450.0 16 53866.0 17 54214.0 18 51252.0 19 50382.0 20 52077.0 21 55045.0 22 59121.0 23 65653.0 24 72544.0 25 79719.0 26 86240.0 27 96666.0 28 115259.0 29 143741.0 30 180733.0 31 234182.0 32 311042.0 33 419350.0 34 570248.0 35 808120.0 36 1182122.0 37 1332759.0 38 434618.0 39 468.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.039692010459994 19.57156755618695 12.557436569138241 23.831303864214824 2 15.176276267732074 22.065359163925024 37.959049816782894 24.799314751560004 3 17.88908412026153 29.12475194858232 28.742950068340967 24.243213862815193 4 12.152466172977345 18.793282858284773 36.95483645673964 32.09941451199824 5 13.0951227500222 39.56606466590103 32.69770310843127 14.641109475645496 6 29.39564861974786 37.9846628169222 18.42523958077987 14.194448982550064 7 26.128774211015592 33.81978486817515 22.282789693606663 17.768651227202593 8 26.812771871418757 33.82405770333987 20.079577491468655 19.283592933772724 9 27.300102021273077 16.803081872034205 19.393356023695052 36.50346008299767 10-11 24.58319772046086 27.76826771841609 25.67578262198064 21.97275193914241 12-13 25.750575620753974 25.910391242418395 25.488993124895927 22.850040011931704 14-15 24.655340313955797 26.146118964889588 23.387096894959267 25.81144382619535 16-17 23.286523424221794 28.139382108600746 25.265273954694834 23.308820512482626 18-19 24.119166177874007 27.653148336355436 26.447641134294464 21.78004435147609 20-21 25.76770895680291 26.710443621079843 24.90439155683744 22.61745586527981 22-23 24.41171480550122 27.13112482144758 23.966222876841623 24.490937496209575 24-25 23.683576665152977 28.001374672850698 24.126414496059983 24.18863416593634 26-27 24.300529668464723 26.990165182495314 24.25696029856287 24.452344850477097 28-29 23.558803359659304 27.05843973375081 25.515367565407555 23.86738934118233 30-31 24.606714382666024 27.261837802739926 24.879324824000083 23.25212299059397 32-33 22.993367510933915 27.903332162566702 24.507729568032037 24.59557075846735 34-35 23.26325366849061 28.05119302595575 25.52116129049648 23.16439201505716 36-37 24.053613317150855 27.748728849409364 24.769448360700537 23.42820947273924 38-39 24.361818308090957 26.995457728495648 24.73654128526902 23.906182678144376 40-41 23.454335408308534 27.998380592386614 25.11358428261134 23.433699716693518 42-43 24.739058774958956 27.37432836250018 24.726555009790747 23.16005785275012 44-45 24.08243345292472 27.769051800856452 24.744074476000886 23.40444027021794 46-47 23.040066415596584 27.560728034855607 26.11897559273247 23.280229956815337 48-49 23.94550953187865 27.778603871930745 25.61764638937456 22.658240206816043 50-51 22.630603940696233 28.26594271271887 25.049270308269428 24.05418303831546 52-53 22.439077276039036 28.11009632616169 24.258322697021715 25.192503700777564 54-55 23.22698542786416 27.708816042601857 24.332425529187624 24.73177300034636 56-57 24.03960421540898 26.583391434770054 24.142464722844167 25.234539626976797 58-59 23.434324118748698 26.756537137475732 25.679241529504598 24.129897214270972 60-61 23.606023621260196 27.411289247192595 24.9619740732624 24.020713058284805 62-63 22.45539953276521 27.505621806965635 24.997038789831645 25.041939870437506 64-65 22.40558211385133 27.384863248902512 25.25594596682945 24.953608670416706 66-67 24.121431478128116 27.021128202670813 24.61123201115317 24.2462083080479 68-69 24.434818697758367 26.48002781104036 24.510433520240234 24.574719970961034 70-71 23.14442358411279 26.931763005834448 25.03897679137318 24.884836618679586 72-73 24.54776331720873 26.602611018762435 24.994363864701903 23.85526179932693 74-75 24.987088012509318 26.240378154639266 25.050798132287937 23.721735700563485 76-77 23.882750329963763 26.881422388659132 25.73244917719451 23.503378104182595 78-79 24.2079669314364 26.76533287491292 25.517946679876204 23.508753513774476 80-81 23.463874541237452 26.554783686599276 26.002396818577484 23.97894495358579 82-83 23.87593456236545 26.71673610869854 24.954020609788092 24.453308719147913 84-85 24.251941938825613 26.87512606710611 24.374576126263122 24.498355867805152 86-87 23.980629584911334 25.95296599186076 24.98052940550184 25.085875017726067 88-89 24.33874457043161 25.302309053720588 25.45242098065938 24.90652539518842 90-91 24.74577849862058 25.854136700356715 25.086382712271536 24.313702088751164 92-93 23.916321591777443 26.218478863267684 25.16085982983876 24.704339715116113 94-95 22.933245622691402 26.683991165643583 25.696033509338623 24.686729702326392 96-97 24.193894501751096 26.44597777986429 24.745983605121253 24.61414411326336 98-99 24.046316430415473 26.635354364061076 24.488094543705976 24.830234661817475 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2043.0 1 1576.5 2 1089.0 3 1040.0 4 888.5 5 740.0 6 681.5 7 684.5 8 695.0 9 664.0 10 624.5 11 600.5 12 598.5 13 625.5 14 703.5 15 865.0 16 1044.0 17 1274.0 18 1644.5 19 2165.5 20 2878.5 21 3921.0 22 5420.0 23 7840.0 24 10750.0 25 14877.0 26 21496.0 27 28772.5 28 38854.5 29 50829.5 30 60080.5 31 71911.0 32 86472.0 33 99907.0 34 112414.0 35 125330.0 36 133629.0 37 143132.5 38 151719.0 39 140690.0 40 133098.0 41 138941.5 42 145733.5 43 162562.5 44 185330.0 45 212369.0 46 251451.5 47 296763.5 48 338635.5 49 380726.5 50 423935.0 51 369064.0 52 281576.0 53 261561.0 54 239003.0 55 224517.0 56 204060.5 57 181821.0 58 176033.5 59 157083.0 60 127941.0 61 94586.0 62 74344.5 63 60123.5 64 43686.5 65 35206.5 66 25911.0 67 18842.0 68 14595.5 69 12338.0 70 11807.0 71 11641.5 72 12371.0 73 11376.5 74 8070.5 75 5727.0 76 4268.0 77 3049.0 78 1957.0 79 1330.0 80 1050.5 81 777.5 82 627.0 83 562.5 84 413.0 85 327.0 86 213.5 87 117.0 88 83.0 89 64.5 90 42.5 91 38.5 92 35.0 93 26.5 94 14.0 95 8.5 96 11.5 97 8.0 98 5.5 99 4.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.002231200600058191 3 0.007067964316962861 4 0.0 5 0.0051212792296637675 6 0.0 7 0.0010032915449926093 8 0.0 9 0.0 10-11 3.743625167882871E-5 12-13 1.647195073868463E-4 14-15 0.0029949001343062965 16-17 2.545665114160352E-4 18-19 0.0 20-21 0.0 22-23 1.1979600537225185E-4 24-25 9.958042946568435E-4 26-27 0.002433356359123866 28-29 0.0020889428436786416 30-31 0.0026205376175180094 32-33 8.760082892845917E-4 34-35 0.0 36-37 2.0964300940144078E-4 38-39 0.062226537540549076 40-41 0.004020653430306204 42-43 4.567222704817102E-4 44-45 2.9949001343062964E-5 46-47 0.001040727796671438 48-49 1.0482150470072039E-4 50-51 9.284190416349519E-4 52-53 4.567222704817102E-4 54-55 0.0 56-57 6.439035288758537E-4 58-59 7.487250335765741E-6 60-61 1.4225775637954907E-4 62-63 8.98470040291889E-5 64-65 7.038015315619796E-4 66-67 0.001445039314802788 68-69 0.0014300648141312565 70-71 9.733425436495462E-5 72-73 0.001909248835620264 74-75 0.001737042077897652 76-77 1.9466850872990925E-4 78-79 2.2461751007297223E-4 80-81 0.0 82-83 4.043115181313501E-4 84-85 4.1928601880288156E-4 86-87 0.0 88-89 2.9949001343062964E-5 90-91 5.016457724963047E-4 92-93 0.0 94-95 0.0 96-97 0.0030248491356493594 98-99 0.005173689982014127 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6678019.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.357805084066076 #Duplication Level Percentage of deduplicated Percentage of total 1 74.81366025536175 23.459921759131745 2 13.838305245664726 8.67877757174727 3 4.870353917781623 4.581708265326412 4 2.0859648778546274 2.6164512020789243 5 1.0960864423344379 1.718543250700537 6 0.6457496124683646 1.2149574288536507 7 0.3929145610709239 0.8624656754527384 8 0.29546177328508216 0.7412026157172901 9 0.21304011321743757 0.6012423310823798 >10 1.3236434193972035 8.08069955183795 >50 0.16533705007251087 3.661011558870944 >100 0.19287874435015995 13.313727283766175 >500 0.038080212367445264 8.306181224298426 >1k 0.026680401574092613 15.42723977400672 >5k 0.0012612553471388964 2.410417321812608 >10k+ 5.821178525256444E-4 4.325453185316446 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 59898 0.8969426412233926 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 53615 0.8028578535041605 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 23567 0.35290405732598246 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 20224 0.3028443015810527 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 19485 0.2917781455847909 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 18479 0.2767137979092303 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 18253 0.2733295607574641 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 16867 0.2525749028267215 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 16190 0.24243716587209468 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 15412 0.23078700434964322 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 11544 0.17286563575215944 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 10818 0.16199414826462757 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 8360 0.12518682561400318 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 8177 0.12244649199111292 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 7545 0.11298260756670503 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 7488 0.11212906102842773 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.9949001343062964E-5 0.0 2 0.0 0.0 0.0 2.9949001343062964E-5 0.0 3 0.0 0.0 0.0 2.9949001343062964E-5 0.0 4 1.4974500671531482E-5 0.0 0.0 5.989800268612593E-5 0.0 5 1.4974500671531482E-5 0.0 0.0 7.487250335765742E-5 0.0 6 4.492350201459445E-5 0.0 0.0 7.487250335765742E-5 0.0 7 4.492350201459445E-5 0.0 0.0 7.487250335765742E-5 0.0 8 4.492350201459445E-5 1.4974500671531482E-5 0.0 7.487250335765742E-5 0.0 9 4.492350201459445E-5 1.4974500671531482E-5 0.0 7.487250335765742E-5 0.0 10-11 4.492350201459445E-5 1.4974500671531482E-5 0.0 7.487250335765742E-5 0.0 12-13 5.9898002686125934E-5 1.4974500671531482E-5 0.0 8.98470040291889E-5 0.0 14-15 8.235975369342316E-5 1.4974500671531482E-5 0.0 1.1230875503648612E-4 0.0 16-17 8.98470040291889E-5 1.4974500671531482E-5 0.0 1.4974500671531484E-4 0.0 18-19 9.733425436495464E-5 1.4974500671531482E-5 0.0 2.2461751007297223E-4 0.0 20-21 1.1230875503648612E-4 1.4974500671531482E-5 0.0 7.337505329050426E-4 0.0 22-23 1.5723225705108058E-4 1.4974500671531482E-5 0.0 0.0034291606537807094 0.0 24-25 1.7220675772261206E-4 1.4974500671531482E-5 0.0 0.009531269677429788 0.0 26-27 1.8718125839414353E-4 1.4974500671531482E-5 0.0 0.01612004997290364 0.0 28-29 2.02155759065675E-4 1.4974500671531482E-5 0.0 0.03474084155795304 0.0 30-31 2.545665114160352E-4 1.4974500671531482E-5 0.0 0.0844936200391164 0.0 32-33 2.545665114160352E-4 1.4974500671531482E-5 0.0 0.17025258538497717 0.0 34-35 2.545665114160352E-4 1.4974500671531482E-5 0.0 0.2801803948146898 0.0 36-37 2.545665114160352E-4 1.4974500671531482E-5 0.0 0.4368136718389091 0.0 38-39 2.545665114160352E-4 1.4974500671531482E-5 0.0 0.6730289326819825 0.0 40-41 2.545665114160352E-4 1.4974500671531482E-5 0.0 0.9825668360632097 0.0 42-43 2.620537617518009E-4 1.4974500671531482E-5 0.0 1.233292388056997 0.0 44-45 2.920027630948639E-4 1.4974500671531482E-5 0.0 1.508523710399746 0.0 46-47 2.994900134306297E-4 1.4974500671531482E-5 0.0 1.8150816881473384 0.0 48-49 2.994900134306297E-4 1.4974500671531482E-5 0.0 2.1232793737184634 0.0 50-51 2.994900134306297E-4 1.4974500671531482E-5 0.0 2.4682169966871914 0.0 52-53 2.994900134306297E-4 1.4974500671531482E-5 0.0 2.834762824124939 0.0 54-55 2.994900134306297E-4 1.4974500671531482E-5 0.0 3.174983479382134 0.0 56-57 3.1446451410216115E-4 1.4974500671531482E-5 0.0 3.5505214944731365 0.0 58-59 3.1446451410216115E-4 1.4974500671531482E-5 0.0 3.967538876424281 0.0 60-61 3.219517644379269E-4 1.4974500671531482E-5 0.0 4.373894413897295 0.0 62-63 3.2943901477369263E-4 1.4974500671531482E-5 0.0 4.785513488356353 0.0 64-65 3.2943901477369263E-4 1.4974500671531482E-5 0.0 5.221862950674444 0.0 66-67 3.2943901477369263E-4 1.4974500671531482E-5 0.0 5.685458516964387 0.0 68-69 3.444135154452241E-4 1.4974500671531482E-5 0.0 6.206264163069916 0.0 70-71 3.593880161167556E-4 1.4974500671531482E-5 0.0 6.833149171932575 0.0 72-73 3.593880161167556E-4 1.4974500671531482E-5 0.0 7.390500086926977 0.0 74-75 3.593880161167556E-4 1.4974500671531482E-5 0.0 7.927665375016154 0.0 76-77 3.593880161167556E-4 2.2461751007297224E-5 0.0 8.513071017018671 0.0 78-79 3.593880161167556E-4 2.9949001343062964E-5 0.0 9.096889361950003 0.0 80-81 3.593880161167556E-4 2.9949001343062964E-5 0.0 9.74593663180653 0.0 82-83 3.593880161167556E-4 2.9949001343062964E-5 0.0 10.463754295997061 0.0 84-85 3.593880161167556E-4 2.9949001343062964E-5 0.0 11.135008750349467 0.0 86-87 3.593880161167556E-4 3.743625167882871E-5 0.0 11.817007408933698 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGA 2665 0.0 33.500607 2 GTACAAG 2815 0.0 32.87179 1 GTACATA 1710 0.0 31.543549 1 GTACTAG 2885 0.0 25.1436 1 GTGATCG 2030 0.0 24.964931 8 GTATAAG 2705 0.0 23.378698 1 GTACAAA 3895 0.0 22.921469 1 TGATCGC 2340 0.0 21.658909 9 TACAAGA 2200 0.0 21.55888 2 CTATTGA 3640 0.0 21.076984 9 TAGGCAT 3990 0.0 20.858976 5 TAAGGTG 2755 0.0 20.589754 4 ATAAGGT 2710 0.0 19.903864 3 CGTTAAC 775 0.0 19.799805 1 CTAGGCA 4695 0.0 18.91519 4 TCTAACG 1800 0.0 18.599817 2 TTTAGGG 4535 0.0 18.456268 2 GTATAAT 2865 0.0 18.340134 1 ATACGGG 2005 0.0 18.321516 3 GGCTATT 4400 0.0 18.280722 7 >>END_MODULE