##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765444_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6678019 Sequences flagged as poor quality 0 Sequence length 99 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30744072456218 33.0 33.0 33.0 33.0 33.0 2 32.13527829136156 33.0 33.0 33.0 33.0 33.0 3 32.189856303194105 33.0 33.0 33.0 33.0 33.0 4 36.123406057994146 37.0 37.0 37.0 33.0 37.0 5 36.08049827351495 37.0 37.0 37.0 33.0 37.0 6 36.09114978558761 37.0 37.0 37.0 33.0 37.0 7 36.00327477355186 37.0 37.0 37.0 33.0 37.0 8 35.975323370598375 37.0 37.0 37.0 33.0 37.0 9 35.698922539753184 37.0 37.0 37.0 33.0 37.0 10-11 35.873960676062765 37.0 37.0 37.0 33.0 37.0 12-13 35.80160576063051 37.0 37.0 37.0 33.0 37.0 14-15 37.145129865608354 37.0 37.0 40.0 33.0 40.0 16-17 37.29037862875203 37.0 37.0 40.0 33.0 40.0 18-19 37.36985526695866 37.0 37.0 40.0 33.0 40.0 20-21 37.40690622473521 37.0 37.0 40.0 33.0 40.0 22-23 37.328513141397174 37.0 37.0 40.0 33.0 40.0 24-25 37.268039219415215 37.0 37.0 40.0 33.0 40.0 26-27 37.10672671042116 37.0 37.0 40.0 33.0 40.0 28-29 37.01397854663187 37.0 37.0 40.0 33.0 40.0 30-31 36.8569561572077 37.0 37.0 40.0 33.0 40.0 32-33 36.6080099502562 37.0 37.0 40.0 33.0 40.0 34-35 36.48813360668785 37.0 37.0 40.0 33.0 40.0 36-37 36.29197543463114 37.0 37.0 40.0 33.0 40.0 38-39 36.09365711598005 37.0 37.0 40.0 33.0 40.0 40-41 35.90299735595242 37.0 37.0 40.0 33.0 40.0 42-43 35.66763002620987 37.0 37.0 40.0 27.0 40.0 44-45 35.377569755342115 37.0 35.0 40.0 27.0 40.0 46-47 35.02413178219469 37.0 33.0 40.0 27.0 40.0 48-49 34.70657630653641 37.0 33.0 40.0 22.0 40.0 50-51 34.37343117771902 37.0 33.0 40.0 22.0 40.0 52-53 34.222459010673674 37.0 33.0 40.0 22.0 40.0 54-55 34.09141872761967 37.0 33.0 40.0 22.0 40.0 56-57 33.90641064363548 37.0 33.0 40.0 22.0 40.0 58-59 33.723602912779974 37.0 33.0 37.0 22.0 40.0 60-61 33.534932515166545 37.0 33.0 37.0 22.0 40.0 62-63 33.208606699082466 37.0 33.0 37.0 22.0 40.0 64-65 33.087588924200425 37.0 33.0 37.0 22.0 40.0 66-67 32.923453946447296 37.0 33.0 37.0 22.0 40.0 68-69 32.69789132076444 37.0 33.0 37.0 22.0 40.0 70-71 32.48783935774965 37.0 33.0 37.0 22.0 37.0 72-73 32.28011548035428 37.0 33.0 37.0 22.0 37.0 74-75 32.078181044408524 35.0 33.0 37.0 22.0 37.0 76-77 31.89753615555751 33.0 33.0 37.0 22.0 37.0 78-79 31.7186308544495 33.0 33.0 37.0 22.0 37.0 80-81 31.59836524873619 33.0 33.0 37.0 18.5 37.0 82-83 31.473516172984834 33.0 33.0 37.0 15.0 37.0 84-85 31.31186853466574 33.0 33.0 37.0 15.0 37.0 86-87 31.208028833101555 33.0 33.0 37.0 15.0 37.0 88-89 31.093574381863842 33.0 33.0 37.0 15.0 37.0 90-91 31.027942118763065 33.0 33.0 37.0 15.0 37.0 92-93 30.90995742899204 33.0 30.0 37.0 15.0 37.0 94-95 30.86509734398779 33.0 30.0 37.0 15.0 37.0 96-97 30.829903823274538 33.0 27.0 37.0 15.0 37.0 98-99 29.993713854363097 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14969177201266604 1101 2 0.3733204565619559 1101 3 0.2577953564883515 1101 4 0.2153148049880187 1101 5 0.07836315305819852 1101 6 0.19016280639323213 1101 7 0.21292843013914364 1101 8 0.16057572223795802 1101 9 0.4161055985964808 1101 10-11 0.32828786718778247 1101 12-13 0.3194579431493807 1101 14-15 0.6024963986242042 1101 16-17 0.470960507467872 1101 18-19 0.31933068326375036 1101 20-21 0.3912983042161713 1101 22-23 0.2945680952051717 1101 24-25 0.3742345716478823 1101 26-27 0.2871031090343408 1101 28-29 0.22543674243518552 1101 30-31 0.29401179475846817 1101 32-33 0.36468447038813423 1101 34-35 0.42094389629021833 1101 36-37 0.4066128294441782 1101 38-39 0.17196758347901664 1101 40-41 0.14518075299392308 1101 42-43 0.27566396340482413 1101 44-45 0.3168154171891402 1101 46-47 0.4303703916505768 1101 48-49 0.4714436916632536 1101 50-51 0.36720987736817534 1101 52-53 0.384175264411013 1101 54-55 0.3692384193722944 1101 56-57 0.46524422829322987 1101 58-59 0.38742032045682606 1101 60-61 0.2390405726849849 1101 62-63 0.37331821495079254 1101 64-65 0.2722697860497263 1101 66-67 0.291698279155284 1101 68-69 0.24065641655631964 1101 70-71 0.2716270580773852 1101 72-73 0.3761859150453546 1101 74-75 0.21223757515628705 1101 76-77 0.21245620836536716 1101 78-79 0.49407760349607344 1101 80-81 0.46105390005449465 1101 82-83 0.4192859164969249 1101 84-85 0.463424899031903 1101 86-87 0.28024597768924764 1101 88-89 0.3915842933746312 1101 90-91 0.29473220999181393 1101 92-93 0.4582198212719035 1101 94-95 0.4346697226783043 1101 96-97 0.3526741659433057 1101 98-99 0.41333276943195685 1102 1 0.1215694321825822 1102 2 0.3248972301226516 1102 3 0.24428245841418317 1102 4 0.21908549139541122 1102 5 0.08321924919115276 1102 6 0.22915585381140602 1102 7 0.19244952103329638 1102 8 0.10597544535881553 1102 9 0.4357653867734612 1102 10-11 0.32390852786426194 1102 12-13 0.2919655879290701 1102 14-15 0.5682850632501157 1102 16-17 0.42218884658764466 1102 18-19 0.3220945117178786 1102 20-21 0.41261177682663686 1102 22-23 0.2591507993592401 1102 24-25 0.4282663926154697 1102 26-27 0.3147423700617864 1102 28-29 0.31368906969376553 1102 30-31 0.3221577111937961 1102 32-33 0.41507875941355366 1102 34-35 0.47761871309334225 1102 36-37 0.43655669860066126 1102 38-39 0.3027741429697741 1102 40-41 0.3189562896183986 1102 42-43 0.32619116529062353 1102 44-45 0.38432598941408 1102 46-47 0.5111750585701245 1102 48-49 0.4812942731658296 1102 50-51 0.42534227601293395 1102 52-53 0.4563283742276596 1102 54-55 0.3773807842165411 1102 56-57 0.4644274630115177 1102 58-59 0.42716876552836425 1102 60-61 0.39918827780145705 1102 62-63 0.42046042234921543 1102 64-65 0.3011506341954444 1102 66-67 0.41167434819627857 1102 68-69 0.39704191156839386 1102 70-71 0.3870199639151224 1102 72-73 0.5199930193550202 1102 74-75 0.3175897564855532 1102 76-77 0.28286888754555406 1102 78-79 0.4818172956270885 1102 80-81 0.43689873176465 1102 82-83 0.42410538936349695 1102 84-85 0.46251903190006516 1102 86-87 0.4377136710959313 1102 88-89 0.43438098612656617 1102 90-91 0.3220134025854975 1102 92-93 0.42042440902559974 1102 94-95 0.42340703137760727 1102 96-97 0.39131950373505475 1102 98-99 0.3019127732493132 1103 1 0.11776358969030554 1103 2 0.3096444654147703 1103 3 0.2145285229243683 1103 4 0.21114029881229612 1103 5 0.13201131070878347 1103 6 0.2102231382182751 1103 7 0.21121488191255366 1103 8 0.1768601318516616 1103 9 0.3615249928939832 1103 10-11 0.2939697360230866 1103 12-13 0.24895808050276713 1103 14-15 0.4728941532851465 1103 16-17 0.45282752866175713 1103 18-19 0.21743339249441362 1103 20-21 0.3187946371400301 1103 22-23 0.2613710786242862 1103 24-25 0.32533132544324417 1103 26-27 0.15060883381962498 1103 28-29 0.06359092031040348 1103 30-31 0.16421492226905343 1103 32-33 0.16227456643183302 1103 34-35 0.2776102001366283 1103 36-37 0.30469402861695016 1103 38-39 0.11541971452968625 1103 40-41 0.06285813322436695 1103 42-43 0.07716189273780572 1103 44-45 0.18272560362692047 1103 46-47 0.2961012412201214 1103 48-49 0.16797798076879644 1103 50-51 0.1832457551081177 1103 52-53 0.21138056365997215 1103 54-55 0.1476881920913229 1103 56-57 0.2182424193751018 1103 58-59 0.20583033844233967 1103 60-61 0.14414985932853597 1103 62-63 0.2214722373316178 1103 64-65 -0.011108899815319262 1103 66-67 0.15367342078475588 1103 68-69 0.07373914475730459 1103 70-71 0.08229241859113756 1103 72-73 0.10446438477318765 1103 74-75 0.09655752042226595 1103 76-77 0.06476555927993033 1103 78-79 0.2909065544790579 1103 80-81 0.14945764643277215 1103 82-83 0.21818242461114323 1103 84-85 0.2771322482152989 1103 86-87 0.2091062556095622 1103 88-89 0.12705308305809382 1103 90-91 0.005730069352317457 1103 92-93 0.05746308188569316 1103 94-95 0.17992895774954576 1103 96-97 0.16965933696122448 1103 98-99 0.14696879851977585 1104 1 0.09504358092465992 1104 2 0.2733565841957244 1104 3 0.19188871265750151 1104 4 0.17779996735516335 1104 5 -5.028122548509373E-4 1104 6 0.14794492500723777 1104 7 0.10947792759000663 1104 8 0.040703425464641896 1104 9 0.3988393189231729 1104 10-11 0.2589299751829657 1104 12-13 0.24760631791031784 1104 14-15 0.42504001797882296 1104 16-17 0.23106459830339077 1104 18-19 0.1609757237953957 1104 20-21 0.24763151097415914 1104 22-23 0.20401746512806795 1104 24-25 0.23716834693777855 1104 26-27 0.12109452160672873 1104 28-29 0.10852697795335331 1104 30-31 0.13074091664995535 1104 32-33 0.1351481240310406 1104 34-35 0.2997587471111842 1104 36-37 0.14155794040219405 1104 38-39 0.03515142445825603 1104 40-41 0.04893204180947208 1104 42-43 0.16657821522174743 1104 44-45 0.19768323848501268 1104 46-47 0.2680173355152391 1104 48-49 0.33827195995297643 1104 50-51 0.24280436244549009 1104 52-53 0.2070024724262325 1104 54-55 0.2059872571119996 1104 56-57 0.24274645984185383 1104 58-59 0.14895220247059626 1104 60-61 0.15148592752865397 1104 62-63 0.1917892153498073 1104 64-65 0.04447731751981365 1104 66-67 0.09062668361499249 1104 68-69 0.02611793200444623 1104 70-71 0.14260575358602523 1104 72-73 0.20248381576373475 1104 74-75 0.09103690050046254 1104 76-77 0.08503545492090936 1104 78-79 0.3071628511659199 1104 80-81 0.19656878093761065 1104 82-83 0.11770123861710857 1104 84-85 0.19358002356672444 1104 86-87 0.1682115344842927 1104 88-89 0.12053264118856077 1104 90-91 0.08356051301037226 1104 92-93 0.0219819093880389 1104 94-95 0.1968479267739518 1104 96-97 0.13153612779360202 1104 98-99 0.07381314155140828 1105 1 0.10962586739355373 1105 2 0.22953400865636553 1105 3 0.19134119361820723 1105 4 0.16766702495178976 1105 5 -0.07370555921146149 1105 6 0.05561753099664202 1105 7 0.11151881485024262 1105 8 -0.05740218021141885 1105 9 0.2937269781849423 1105 10-11 0.2439476295126468 1105 12-13 0.19199655088383594 1105 14-15 0.27261447434747055 1105 16-17 0.2632393161057678 1105 18-19 0.10967034396873032 1105 20-21 0.3018565025252826 1105 22-23 0.11206522305180755 1105 24-25 0.23671226230452191 1105 26-27 0.03346454020473999 1105 28-29 0.06288048022663162 1105 30-31 0.08584847969923715 1105 32-33 0.07950046465093408 1105 34-35 0.17271569240440243 1105 36-37 0.0636320065912841 1105 38-39 0.015859318279964896 1105 40-41 -0.033900168086105964 1105 42-43 0.05687241114178221 1105 44-45 -0.021702267250852003 1105 46-47 0.16280378359513747 1105 48-49 0.050876889591442875 1105 50-51 -0.03875287555146656 1105 52-53 -0.09894785065898049 1105 54-55 -0.053816566492955076 1105 56-57 0.06026196910970327 1105 58-59 -0.01710838736366327 1105 60-61 -0.040389984182624517 1105 62-63 -0.05963490723991072 1105 64-65 -0.24487526810725768 1105 66-67 -0.05861072493124908 1105 68-69 -0.1512503072791418 1105 70-71 0.004244562417355269 1105 72-73 -0.037830548919913554 1105 74-75 -0.11264080908348362 1105 76-77 -0.09485658361150229 1105 78-79 -0.006580630061037596 1105 80-81 -0.0689119794745423 1105 82-83 -0.14193894644726868 1105 84-85 -0.22067225949166414 1105 86-87 -0.15978975789182925 1105 88-89 -0.06528972702045266 1105 90-91 -0.11620792630722576 1105 92-93 -0.17323439746682823 1105 94-95 0.010737853914633888 1105 96-97 -0.14199647856689168 1105 98-99 -0.10439788911649472 1106 1 0.06839042153643504 1106 2 0.22560332146683493 1106 3 0.16089800905956508 1106 4 0.16874021104125347 1106 5 -0.0028226451353745574 1106 6 0.14061645485670482 1106 7 0.13902653786377783 1106 8 0.02873408958277679 1106 9 0.3396751516462331 1106 10-11 0.23638607533449374 1106 12-13 0.20892568214438256 1106 14-15 0.36117365100538024 1106 16-17 0.2649706297140426 1106 18-19 0.20569135851406628 1106 20-21 0.2754373869809186 1106 22-23 0.1074178828648158 1106 24-25 0.25016303807568363 1106 26-27 0.11815576534186789 1106 28-29 0.15768710907217098 1106 30-31 0.11264714532025266 1106 32-33 0.10628468711407635 1106 34-35 0.2089403023446792 1106 36-37 0.011769115431299326 1106 38-39 0.04978203315324947 1106 40-41 0.039780652280661855 1106 42-43 0.08382872872192593 1106 44-45 0.08446359356241828 1106 46-47 0.2253715818017028 1106 48-49 0.35590595879628495 1106 50-51 0.1793755297994295 1106 52-53 0.2269280467852397 1106 54-55 0.2432665888663479 1106 56-57 0.28288396598541254 1106 58-59 0.2134809627197427 1106 60-61 0.24700090116434126 1106 62-63 0.28510174997233406 1106 64-65 0.048808523176795404 1106 66-67 0.2533848470612412 1106 68-69 0.1438499684202128 1106 70-71 0.1413380817494101 1106 72-73 0.19255498096933366 1106 74-75 0.16563412757042073 1106 76-77 0.16090931489591043 1106 78-79 0.26911851899912165 1106 80-81 0.26193256567176704 1106 82-83 0.18431871166315972 1106 84-85 0.16182299378272802 1106 86-87 0.13350086493300495 1106 88-89 0.07620242918439146 1106 90-91 0.04634967633418796 1106 92-93 -0.043898210948079 1106 94-95 0.21866601878790348 1106 96-97 0.1740997706370777 1106 98-99 0.029775412627561337 1107 1 0.10014978669047281 1107 2 0.23114815235962993 1107 3 0.15435712886817043 1107 4 0.15379949033791007 1107 5 -0.0019524867047735484 1107 6 0.044545068557361844 1107 7 0.06259870823510738 1107 8 -0.004503164891538347 1107 9 0.27018119575974 1107 10-11 0.1845116401066491 1107 12-13 0.14508578461147437 1107 14-15 0.2648522666586999 1107 16-17 0.18219569419720472 1107 18-19 0.06902015789858496 1107 20-21 0.19092080153029656 1107 22-23 0.0540582451931968 1107 24-25 0.1645755541176186 1107 26-27 -0.06479027097898182 1107 28-29 0.022485020151194135 1107 30-31 0.03583548466779973 1107 32-33 -0.08245041401618636 1107 34-35 0.08440403323254486 1107 36-37 0.07026949402391125 1107 38-39 -0.01041050270678312 1107 40-41 -0.056394544698655125 1107 42-43 -0.008535091718371746 1107 44-45 -0.0013902889908194993 1107 46-47 0.07551745749295691 1107 48-49 0.2598809296264051 1107 50-51 0.09701388433273905 1107 52-53 0.10479730713799285 1107 54-55 0.12418527151426417 1107 56-57 0.19103845730595026 1107 58-59 0.12565814135838593 1107 60-61 0.15002955005810747 1107 62-63 0.2302501600035356 1107 64-65 0.03207788967967673 1107 66-67 0.11150957518954385 1107 68-69 0.023011182632288296 1107 70-71 0.04797275767833753 1107 72-73 0.1407626799103241 1107 74-75 0.09072553128758187 1107 76-77 0.07942644589240899 1107 78-79 0.09375250233113519 1107 80-81 0.0951780758082279 1107 82-83 -0.0017972116521960402 1107 84-85 0.014467103809959525 1107 86-87 0.08742101456157769 1107 88-89 -0.02829381620730942 1107 90-91 -0.040050456176984994 1107 92-93 -0.03930407672276459 1107 94-95 0.1750149515487749 1107 96-97 0.06299307180843883 1107 98-99 0.018229220423577885 1108 1 0.041472486377791995 1108 2 0.21707246005955483 1108 3 0.1399955757661857 1108 4 0.03413200004137451 1108 5 -0.061434073341466444 1108 6 -0.007530730003274755 1108 7 -0.01025154558172403 1108 8 -0.06335506375742739 1108 9 0.22484724100109332 1108 10-11 0.14564424282126254 1108 12-13 0.10617813892839223 1108 14-15 0.13776274891657891 1108 16-17 0.13810405120703706 1108 18-19 -0.09368145898626778 1108 20-21 0.08211897390025058 1108 22-23 -0.0527736354580739 1108 24-25 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-0.39780511421621867 2215 92-93 -0.18835259635493884 2215 94-95 -0.2713173113105505 2215 96-97 -0.3038285124476374 2215 98-99 -0.14343323593926627 2216 1 -0.24478548047984106 2216 2 -0.02688902233145285 2216 3 -0.12267537705635334 2216 4 -0.21312785025330072 2216 5 -0.2200976101750598 2216 6 -0.2512125719172502 2216 7 -0.233124788636637 2216 8 -0.2328713537151117 2216 9 0.037060947860993565 2216 10-11 -0.18723768309426703 2216 12-13 -0.06589004681224964 2216 14-15 -0.4324842033650995 2216 16-17 -0.548615343734582 2216 18-19 -0.5195136729461751 2216 20-21 -0.8072869840546275 2216 22-23 -0.6923278509716582 2216 24-25 -0.567074325839954 2216 26-27 -0.6995147030885462 2216 28-29 -0.7867984424352414 2216 30-31 -0.4024102173543582 2216 32-33 -0.5000091835239218 2216 34-35 -0.43521068640529137 2216 36-37 -0.4950180300318152 2216 38-39 -0.476484358659917 2216 40-41 -0.4480354940122737 2216 42-43 -0.5265195049274922 2216 44-45 -0.3789745034112926 2216 46-47 -0.7111012276274593 2216 48-49 -0.8245805005856965 2216 50-51 -0.43811561084801554 2216 52-53 -0.38963085836536493 2216 54-55 -0.3355748266587142 2216 56-57 -0.45150900972456043 2216 58-59 -0.4630078694272157 2216 60-61 -0.4273524765401646 2216 62-63 -0.5080758697364942 2216 64-65 -0.500101397468022 2216 66-67 -0.4668607897826291 2216 68-69 -0.37522633943285655 2216 70-71 -0.49342646201213114 2216 72-73 -0.4537574531754984 2216 74-75 -0.4014367827444971 2216 76-77 -0.35975652248899337 2216 78-79 -0.2411180944261666 2216 80-81 -0.22731158917284944 2216 82-83 -0.23376548829769916 2216 84-85 -0.28195952523126877 2216 86-87 -0.3979722036821727 2216 88-89 -0.21491640236929754 2216 90-91 -0.26205613474397893 2216 92-93 -0.2511703662240805 2216 94-95 -0.33640400867730236 2216 96-97 -0.3877222653495167 2216 98-99 -0.3860647003281734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 25.0 10 139.0 11 284.0 12 550.0 13 1019.0 14 2692.0 15 7136.0 16 15526.0 17 27596.0 18 42081.0 19 54217.0 20 65765.0 21 79568.0 22 88931.0 23 77321.0 24 59720.0 25 55123.0 26 63781.0 27 80232.0 28 105146.0 29 136852.0 30 178600.0 31 233196.0 32 305262.0 33 397823.0 34 526245.0 35 744387.0 36 1194564.0 37 1584180.0 38 549299.0 39 753.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.69587975162605 19.601114818115406 13.292551957823653 24.410453472434888 2 14.986959136631347 22.01499309918484 37.622785988469886 25.375261775713927 3 17.846819543340622 28.06760507869175 30.082873379066456 24.00270199890117 4 12.39177366820909 18.321825679142272 37.33412558424886 31.95227506839978 5 12.731074976807708 38.13960000688843 33.75249424778728 15.376830768516584 6 29.570730481599412 37.723133162693905 18.023353931757306 14.682782423949378 7 24.761145483413568 33.14464963337181 23.719099930683036 18.375104952531583 8 27.284064331053866 31.559703558794904 21.071578262954926 20.0846538471963 9 27.432831985470518 13.499164087447655 20.956696075853298 38.11130785122853 10-11 24.869650715279487 25.59887146173139 27.180306015900825 22.3511718070883 12-13 26.576564097826015 23.764083330700316 27.490974494082753 22.168378077390916 14-15 24.53070761659466 23.661018563394652 26.7189205382304 25.089353281780287 16-17 22.23907759039284 25.932534683393417 28.391709604801935 23.436678121411813 18-19 22.895449982996453 24.502663140071927 31.431821323060028 21.170065553871588 20-21 23.79622609639176 23.890565450622407 30.529083550076752 21.78412490290908 22-23 22.613510084352857 24.00005151228231 29.691058081745496 23.695380321619332 24-25 22.366453285023596 24.88773242484036 29.769913802281785 22.975900487854258 26-27 23.130047997767004 23.849101058262935 29.835015443951267 23.185835500018793 28-29 22.188047084022973 23.79300657874738 31.117499066714245 22.9014472705154 30-31 23.342124363527567 23.83643263069482 30.38356135255081 22.437881653226803 32-33 22.09450886557825 24.24622481607195 29.872384310377075 23.786882007972725 34-35 22.537140130928048 24.388684728210567 30.75038420825098 22.323790932610404 36-37 23.41910827147991 24.1703490211693 29.645041441181885 22.765501266168904 38-39 23.567498085884452 23.406140353898365 29.64932414837394 23.377037411843244 40-41 22.834084479244517 24.195326488289417 29.814111041013806 23.156477991452256 42-43 24.357155917046658 23.772798490091148 28.905316082508897 22.964729510353298 44-45 23.869867590700313 24.186386052343025 28.409774381202062 23.5339719757546 46-47 23.178527943691087 24.163835113377186 29.14210037437749 23.51553656855424 48-49 24.33732967815755 24.83028275301403 27.76802521825709 23.06436235057133 50-51 22.915882689162757 25.560005145238428 26.977948101076084 24.546164064522728 52-53 22.59628678109253 25.575439317107584 26.186781938592002 25.64149196320788 54-55 23.276790617097674 25.48251959151359 26.095283646242994 25.14540614514574 56-57 23.9077126617123 24.360643745701804 25.93872593337998 25.792917659205916 58-59 23.02068173211247 24.425821489875965 27.48751538442763 25.065981393583936 60-61 23.290170333447687 25.017493960409514 26.79351466355516 24.89882104258763 62-63 22.246979231415782 25.00875633926768 26.813445723948977 25.930818705367564 64-65 22.13051505244295 24.873154748436622 27.126712277997413 25.869617921123016 66-67 23.61682409109648 24.641791225811126 26.558736954776556 25.18264772831584 68-69 23.740312000147952 23.998394109177966 26.599064213694888 25.66222967697919 70-71 22.537240001704102 24.2809355884295 27.16657478537176 26.015249624494636 72-73 23.76731033559503 23.95145925760319 27.063774451674966 25.21745595512681 74-75 23.978458282313962 23.763911123942595 27.004175939002266 25.253454654741176 76-77 22.886016047573392 24.504100692136397 27.5336068974946 25.076276362795614 78-79 23.14607631635831 24.646889773604393 27.15241595941047 25.054617950626824 80-81 22.16886170157009 24.56896951908894 27.419994159884396 25.84217461945657 82-83 22.447300614149196 24.743707677381572 26.414944312078177 26.394047396391056 84-85 22.796663202066362 24.870676468575486 25.799140433712452 26.533519895645703 86-87 22.38346431778646 24.040639896352495 26.44174866828022 27.134147117580827 88-89 22.54169986638253 23.569242615212683 26.83808626480398 27.050971253600807 90-91 22.974185507531978 23.93263215460094 26.500372192663274 26.592810145203806 92-93 22.112111143032067 24.328778270169146 26.363465992987223 27.195644593811565 94-95 21.1415600875953 24.613318929784796 27.06918734755878 27.17593363506112 96-97 22.42235867193461 24.15726446220908 26.280550035980088 27.139826829876224 98-99 22.157102278385253 24.008504617911388 26.287159410597667 27.547233693105692 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 302.0 1 238.5 2 437.0 3 666.0 4 461.0 5 423.0 6 802.0 7 1966.5 8 3559.5 9 3579.5 10 2200.0 11 1333.5 12 1230.5 13 1398.0 14 1780.5 15 2418.5 16 3145.5 17 3960.5 18 5042.0 19 6264.5 20 7753.0 21 9479.5 22 11514.0 23 14418.5 24 17676.5 25 21831.0 26 27825.5 27 34036.0 28 43120.0 29 53624.5 30 61258.5 31 71971.0 32 85587.0 33 97674.5 34 108455.5 35 120443.5 36 128157.0 37 137522.5 38 145948.5 39 135094.5 40 127971.5 41 132261.5 42 137311.0 43 154053.0 44 176265.0 45 202196.5 46 239919.0 47 284032.0 48 324046.0 49 368286.5 50 424464.0 51 382646.0 52 296931.5 53 268750.0 54 239889.0 55 226158.5 56 208229.0 57 186606.5 58 181208.0 59 161676.0 60 129017.5 61 93887.5 62 74170.5 63 59660.5 64 42943.0 65 34913.5 66 25526.5 67 18422.5 68 14246.5 69 12181.0 70 11983.0 71 12102.5 72 13720.0 73 12025.0 74 7332.0 75 5460.5 76 4456.5 77 3006.0 78 1401.0 79 896.5 80 831.5 81 679.5 82 596.5 83 479.0 84 297.5 85 217.5 86 104.5 87 35.0 88 24.5 89 20.5 90 15.0 91 8.5 92 3.5 93 2.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05341404389535279 2 0.008191051867327721 3 0.0 4 0.0 5 0.0023060731034158484 6 0.0 7 0.0 8 0.0 9 0.0042976816927295354 10-11 0.0 12-13 0.0 14-15 0.002583101365839181 16-17 0.004365066945751427 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 7.711867845838714E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.8718125839414353E-4 54-55 0.0 56-57 0.001070676798014501 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0015798098208465712 70-71 2.7702826242333243E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 6.064672771970251E-4 80-81 0.0010332405463356722 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.893370174598185E-4 92-93 9.80829793985312E-4 94-95 5.241075235036019E-4 96-97 2.920027630948639E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6678019.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.251531369693296 #Duplication Level Percentage of deduplicated Percentage of total 1 72.58203645931643 20.505536799066 2 14.913597816720902 8.4266395310816 3 5.327744441066825 4.515508176195255 4 2.3182212448064363 2.6197320087815394 5 1.1947068700635248 1.6876149308593875 6 0.7030512956841174 1.1917365440714014 7 0.46036393125443875 0.9104190231717072 8 0.31777271135632373 0.7182052578652538 9 0.22920742743146627 0.5827914743622092 >10 1.4580059242293502 8.055739841222358 >50 0.19254415330156974 3.8200183879189837 >100 0.22470921966998245 13.985890908036241 >500 0.0445703345065284 8.817047710859049 >1k 0.031042940839138784 16.163929930947237 >5k 0.0016707138298842018 2.9673562868366523 >10k+ 7.545159231735105E-4 5.031833188725327 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 59360 0.8888863598621088 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 55826 0.8359664744889165 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25100 0.3758599668554402 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 22820 0.34171810532434843 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 19947 0.2986963648950385 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 19526 0.29239210011232375 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 18216 0.2727755042326175 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 17724 0.265408049902224 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 17098 0.25603401248184526 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 16876 0.2527096733327653 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16844 0.2522304893112763 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 16191 0.24245214037276622 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12713 0.19037082703717975 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 12238 0.1832579392182023 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 9947 0.14895135817972366 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 9363 0.14020624978754928 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 7923 0.11864296882054394 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 7722 0.11563309418556611 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 7457 0.11166485150761027 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 6913 0.10351872314229714 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 6688 0.10014946049120256 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.9949001343062964E-5 0.0 2 0.0 0.0 0.0 2.9949001343062964E-5 0.0 3 0.0 0.0 0.0 2.9949001343062964E-5 0.0 4 0.0 0.0 0.0 5.989800268612593E-5 0.0 5 0.0 0.0 0.0 8.98470040291889E-5 0.0 6 0.0 0.0 0.0 8.98470040291889E-5 0.0 7 0.0 0.0 0.0 8.98470040291889E-5 0.0 8 0.0 0.0 0.0 8.98470040291889E-5 0.0 9 0.0 0.0 0.0 8.98470040291889E-5 0.0 10-11 0.0 0.0 0.0 8.98470040291889E-5 0.0 12-13 0.0 0.0 0.0 1.0482150470072038E-4 0.0 14-15 7.487250335765741E-6 0.0 0.0 1.272832557080176E-4 0.0 16-17 1.4974500671531482E-5 0.0 0.0 1.7220675772261206E-4 0.0 18-19 1.4974500671531482E-5 0.0 0.0 2.545665114160352E-4 0.0 20-21 1.4974500671531482E-5 0.0 0.0 7.487250335765741E-4 0.0 22-23 1.4974500671531482E-5 0.0 0.0 0.0034740841557953043 0.0 24-25 2.2461751007297224E-5 0.0 0.0 0.009591167680115914 0.0 26-27 4.492350201459445E-5 0.0 0.0 0.016172460725254002 0.0 28-29 4.492350201459445E-5 0.0 0.0 0.03500289531970484 0.0 30-31 4.492350201459445E-5 0.0 0.0 0.08520490882101414 0.0 32-33 4.492350201459445E-5 0.0 0.0 0.1715628541937362 0.0 34-35 4.492350201459445E-5 0.0 0.0 0.28249395516844145 0.0 36-37 4.492350201459445E-5 0.0 0.0 0.4394117477054198 0.0 38-39 4.492350201459445E-5 0.0 0.0 0.6755745977961429 0.0 40-41 4.492350201459445E-5 0.0 0.0 0.9842889036404359 0.0 42-43 4.492350201459445E-5 0.0 0.0 1.2347898381241502 0.0 44-45 4.492350201459445E-5 0.0 0.0 1.5097291577038041 0.0 46-47 4.492350201459445E-5 0.0 0.0 1.8158154386802434 0.0 48-49 4.492350201459445E-5 0.0 0.0 2.1233242972204778 0.0 50-51 5.241075235036019E-5 0.0 0.0 2.4676329911610013 0.0 52-53 5.989800268612593E-5 0.0 0.0 2.833250399557114 0.0 54-55 5.989800268612593E-5 0.0 0.0 3.17269238077939 0.0 56-57 5.989800268612593E-5 0.0 0.0 3.5472570533267427 0.0 58-59 5.989800268612593E-5 0.0 0.0 3.96342088873961 0.0 60-61 5.989800268612593E-5 0.0 0.0 4.3696191939555735 0.0 62-63 5.989800268612593E-5 0.0 0.0 4.781957044446863 0.0 64-65 7.487250335765742E-5 0.0 0.0 5.220717401373072 0.0 66-67 7.487250335765742E-5 0.0 0.0 5.686626528016767 0.0 68-69 7.487250335765742E-5 0.0 0.0 6.210194969496193 0.0 70-71 7.487250335765742E-5 0.0 0.0 6.838719686182385 0.0 72-73 7.487250335765742E-5 0.0 0.0 7.398241903774158 0.0 74-75 8.235975369342316E-5 0.0 0.0 7.938095114733876 0.0 76-77 8.98470040291889E-5 0.0 0.0 8.525200362562611 0.0 78-79 8.98470040291889E-5 0.0 0.0 9.110965392581242 0.0 80-81 8.98470040291889E-5 0.0 0.0 9.762303761040512 0.0 82-83 8.98470040291889E-5 0.0 0.0 10.482300215078752 0.0 84-85 8.98470040291889E-5 0.0 0.0 11.154962272494283 0.0 86-87 8.98470040291889E-5 0.0 0.0 11.839791111705432 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 480 0.0 53.283356 2 TCACGCA 735 0.0 36.06319 4 TATTCGC 410 0.0 27.221405 9 ATCACGC 940 0.0 27.20893 3 TAACGCA 590 0.0 26.79807 4 CTATTGA 3265 0.0 23.928156 9 TAGTACT 3535 0.0 23.021057 4 GGGCTAT 3960 0.0 22.663607 6 CTGTGCG 3450 0.0 22.37547 9 AGTACTT 42370 0.0 21.501667 12-13 AGGGCTA 4050 0.0 20.43815 5 GGCTATT 4240 0.0 20.399239 7 CATGGGT 5095 0.0 20.353415 4 ACTGTGC 4430 0.0 20.049776 8 GTACTAG 3015 0.0 19.907042 1 TAGGCAT 4000 0.0 19.182297 5 CTAGTAC 4575 0.0 19.007595 3 TACTGTG 4885 0.0 18.943289 7 GTGATCG 1920 0.0 18.891798 8 TCTAACG 1540 0.0 18.72152 2 >>END_MODULE