##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765443_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7219820
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23011598627112	33.0	31.0	34.0	31.0	34.0
2	32.27107767229654	34.0	31.0	34.0	31.0	34.0
3	32.30632882814253	34.0	31.0	34.0	30.0	34.0
4	35.76362333132959	37.0	35.0	37.0	35.0	37.0
5	35.774323321080026	37.0	35.0	37.0	35.0	37.0
6	35.87243103013648	37.0	37.0	37.0	35.0	37.0
7	35.82185566953193	37.0	37.0	37.0	35.0	37.0
8	35.853920734865966	37.0	37.0	37.0	35.0	37.0
9	37.55474111542947	39.0	38.0	39.0	35.0	39.0
10-11	37.479989805840034	39.0	38.0	39.0	35.0	39.0
12-13	37.46456947680136	39.0	38.0	39.0	35.0	39.0
14-15	38.84781781263245	41.0	39.0	41.0	35.5	41.0
16-17	38.78996110706361	40.5	38.5	41.0	35.0	41.0
18-19	38.76842642891374	41.0	38.5	41.0	35.0	41.0
20-21	38.75324280937752	40.5	39.0	41.0	35.0	41.0
22-23	38.67514584851146	40.5	39.0	41.0	35.0	41.0
24-25	38.608440653645104	40.5	38.0	41.0	34.0	41.0
26-27	38.478856536589554	40.0	38.0	41.0	34.0	41.0
28-29	38.336323620256465	40.0	38.0	41.0	34.0	41.0
30-31	38.11610947364339	40.0	38.0	41.0	34.0	41.0
32-33	37.857355446534676	40.0	38.0	41.0	33.0	41.0
34-35	37.73075983888795	40.0	38.0	41.0	33.0	41.0
36-37	37.60172490449901	40.0	37.0	41.0	33.0	41.0
38-39	37.41176587504952	40.0	37.0	41.0	32.5	41.0
40-41	37.17349726170458	40.0	37.0	41.0	32.0	41.0
42-43	36.93245108603816	40.0	36.5	41.0	31.0	41.0
44-45	36.58505101235211	40.0	36.0	41.0	30.5	41.0
46-47	36.20471092077088	39.0	35.5	41.0	30.0	41.0
48-49	35.871256762633976	39.0	35.0	41.0	28.5	41.0
50-51	34.6273126615345	38.0	34.0	40.0	26.0	40.5
52-53	34.59098038455252	38.0	34.5	39.5	26.5	40.0
54-55	35.43685791335518	39.0	35.0	40.5	28.0	41.0
56-57	35.45903083456374	39.0	35.0	41.0	28.0	41.0
58-59	35.261257205858314	39.0	35.0	41.0	28.0	41.0
60-61	34.90603387619082	38.0	35.0	40.5	27.0	41.0
62-63	34.524937602322495	37.0	35.0	40.0	26.0	41.0
64-65	34.23308586640664	37.0	34.5	40.0	26.0	41.0
66-67	33.898383823973454	36.0	34.0	39.0	26.0	41.0
68-69	33.63169317240596	36.0	34.0	39.0	25.5	41.0
70-71	33.24208359765202	35.0	34.0	39.0	25.0	41.0
72-73	32.83621177536282	35.0	34.0	37.5	24.0	39.5
74-75	32.45841108227074	35.0	34.0	37.0	23.5	39.0
76-77	32.08904418669718	35.0	34.0	36.5	22.0	39.0
78-79	31.760079891188425	35.0	33.0	36.0	20.0	37.5
80-81	31.450729935095335	35.0	33.0	35.5	20.0	37.0
82-83	31.21137680717802	35.0	33.0	35.0	18.5	37.0
84-85	30.960579488131284	35.0	33.0	35.0	16.0	36.0
86-87	30.71235411686164	35.0	33.0	35.0	9.0	36.0
88-89	30.4858206298772	35.0	32.5	35.0	4.5	36.0
90-91	30.2362939796283	35.0	32.0	35.0	2.0	35.0
92-93	29.945522603056588	35.0	32.0	35.0	2.0	35.0
94-95	29.62434458199789	35.0	31.0	35.0	2.0	35.0
96-97	29.30246917513179	35.0	31.0	35.0	2.0	35.0
98-99	28.971222066478113	35.0	31.0	35.0	2.0	35.0
100	28.39390123299473	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5084481044965372
1101	2	0.5692220597967435
1101	3	0.582725594107572
1101	4	0.4998607053846911
1101	5	0.5310297515768454
1101	6	0.4600686122900228
1101	7	0.494332078989963
1101	8	0.4229717896153531
1101	9	0.5060880819122318
1101	10-11	0.5034165677736624
1101	12-13	0.5668748042367753
1101	14-15	0.7240167337277512
1101	16-17	0.7158375139700155
1101	18-19	0.6546205259285713
1101	20-21	0.6482280551224093
1101	22-23	0.6919567599534773
1101	24-25	0.6839156585292656
1101	26-27	0.7051179329779629
1101	28-29	0.6743813148288496
1101	30-31	0.634381078155883
1101	32-33	0.6605258379145127
1101	34-35	0.7573367776511972
1101	36-37	0.7147806415533964
1101	38-39	0.7052085201135228
1101	40-41	0.7589918822947936
1101	42-43	0.6851856793335358
1101	44-45	0.7330742747779269
1101	46-47	0.778038036780714
1101	48-49	0.6174043752093894
1101	50-51	0.6664448807757424
1101	52-53	0.725330991674447
1101	54-55	0.8185320649564218
1101	56-57	0.8762838650839981
1101	58-59	0.7932135086751231
1101	60-61	0.8698822624446052
1101	62-63	0.9251038684563184
1101	64-65	0.8222910711095608
1101	66-67	0.8109668214895862
1101	68-69	0.8381510329339079
1101	70-71	0.7469203965595099
1101	72-73	0.7366080558524288
1101	74-75	0.5728359491426644
1101	76-77	0.6088872543509538
1101	78-79	0.5686222077745775
1101	80-81	0.5384912098098056
1101	82-83	0.5022322084419883
1101	84-85	0.5414577070308901
1101	86-87	0.5609209384136662
1101	88-89	0.5349424173467163
1101	90-91	0.5164562147094252
1101	92-93	0.5822531994153195
1101	94-95	0.4902229427208624
1101	96-97	0.4301640772374249
1101	98-99	0.37031944360974833
1101	100	0.4253806839626719
1102	1	0.5853248081404274
1102	2	0.6183267642727515
1102	3	0.6620005452517432
1102	4	0.5261590572527766
1102	5	0.49970614639192235
1102	6	0.529819882826537
1102	7	0.5690262322083015
1102	8	0.5131313348860189
1102	9	0.5797143683766706
1102	10-11	0.6094355305251753
1102	12-13	0.6238750461920262
1102	14-15	0.775490883122032
1102	16-17	0.8458448377643677
1102	18-19	0.8319248953311558
1102	20-21	0.8182193336055832
1102	22-23	0.8400390779364244
1102	24-25	0.825469662658115
1102	26-27	0.8470114954617856
1102	28-29	0.7956691064286971
1102	30-31	0.7652186558838423
1102	32-33	0.8268671100400908
1102	34-35	0.916385585441418
1102	36-37	0.8798952765083143
1102	38-39	0.9256106441902929
1102	40-41	0.9107830670355668
1102	42-43	0.9272865457721906
1102	44-45	1.0059564334747861
1102	46-47	0.9020085395442052
1102	48-49	0.999029749354591
1102	50-51	0.8602436305876324
1102	52-53	0.9106330815680863
1102	54-55	1.0421442351023984
1102	56-57	1.0769130166081098
1102	58-59	1.0532359590834517
1102	60-61	1.0205608329571945
1102	62-63	1.060650108576084
1102	64-65	1.0735978059199525
1102	66-67	1.01158007900937
1102	68-69	0.9785841620233526
1102	70-71	0.9962401263892602
1102	72-73	0.9368026412112727
1102	74-75	0.9302107196549585
1102	76-77	0.9289507055231496
1102	78-79	0.9474117319396349
1102	80-81	0.9747431742544563
1102	82-83	0.9741156130116728
1102	84-85	0.9910342921126336
1102	86-87	0.9726903180053696
1102	88-89	0.8853398047727268
1102	90-91	0.8728437249870993
1102	92-93	0.9843937638862172
1102	94-95	0.8249029239832417
1102	96-97	0.8363202271281018
1102	98-99	0.8353080901679384
1102	100	0.8398987933571185
1103	1	0.5454384356897677
1103	2	0.5552249457996297
1103	3	0.5979830687853394
1103	4	0.4706674616986959
1103	5	0.5350436657356852
1103	6	0.5221999323923612
1103	7	0.5541211358804645
1103	8	0.5202161448307194
1103	9	0.5775570852652905
1103	10-11	0.6162279110197915
1103	12-13	0.5894034028523265
1103	14-15	0.7670938123120763
1103	16-17	0.7932803341664609
1103	18-19	0.7724049958869585
1103	20-21	0.7741260969624761
1103	22-23	0.7952864683556484
1103	24-25	0.7628206504777424
1103	26-27	0.776982541799093
1103	28-29	0.7161854848938844
1103	30-31	0.7807007273740822
1103	32-33	0.8201671091499847
1103	34-35	0.763178493637831
1103	36-37	0.7016169131722663
1103	38-39	0.7415118571414965
1103	40-41	0.7895368070426159
1103	42-43	0.8229605989925091
1103	44-45	0.7839881756918246
1103	46-47	0.759284943389936
1103	48-49	0.8239058983775749
1103	50-51	0.8160795992510401
1103	52-53	0.8738956583550532
1103	54-55	0.9050222253596232
1103	56-57	0.8903900755670193
1103	58-59	0.8813202489557739
1103	60-61	0.7945060015610963
1103	62-63	0.788804016939082
1103	64-65	0.7949231893938489
1103	66-67	0.7879966086643009
1103	68-69	0.7272612517542711
1103	70-71	0.6659422228277165
1103	72-73	0.6399482207867919
1103	74-75	0.6208436312083236
1103	76-77	0.6248857486103176
1103	78-79	0.6258271070084582
1103	80-81	0.5931370470643387
1103	82-83	0.505428770334202
1103	84-85	0.5234627122243012
1103	86-87	0.5671990403439402
1103	88-89	0.4834464361405928
1103	90-91	0.502710057485011
1103	92-93	0.5485859541538716
1103	94-95	0.5407645910885996
1103	96-97	0.6290863573191281
1103	98-99	0.6192685621240805
1103	100	0.6227107827420468
1104	1	0.4139882965087054
1104	2	0.49270821219855776
1104	3	0.5345000361255998
1104	4	0.48071665317359447
1104	5	0.47819616993236735
1104	6	0.4041233934322861
1104	7	0.43947100094920444
1104	8	0.39468916920334607
1104	9	0.5216498584745324
1104	10-11	0.5326124969826864
1104	12-13	0.5102574539774594
1104	14-15	0.6126596736409908
1104	16-17	0.6310533840379051
1104	18-19	0.6307725932605734
1104	20-21	0.6229111844021844
1104	22-23	0.6201680050845724
1104	24-25	0.6455380279240259
1104	26-27	0.687646626710162
1104	28-29	0.671247000798779
1104	30-31	0.681850033593669
1104	32-33	0.6666493343470776
1104	34-35	0.5887240621552863
1104	36-37	0.5512503676250091
1104	38-39	0.6461011136383874
1104	40-41	0.6627011575664028
1104	42-43	0.624738876122187
1104	44-45	0.6256652012543924
1104	46-47	0.6473177624970603
1104	48-49	0.6496698005471018
1104	50-51	0.5959384526540674
1104	52-53	0.581462699004426
1104	54-55	0.5973662044505943
1104	56-57	0.6539007319491574
1104	58-59	0.5753981478529511
1104	60-61	0.565438674049993
1104	62-63	0.5784395431709939
1104	64-65	0.559471063429676
1104	66-67	0.4610879156913441
1104	68-69	0.473198705272047
1104	70-71	0.5004129098084391
1104	72-73	0.5384751326671235
1104	74-75	0.5588788408298768
1104	76-77	0.5411938381111838
1104	78-79	0.5807199588390652
1104	80-81	0.5756982442339691
1104	82-83	0.5504517192728109
1104	84-85	0.5609066679413495
1104	86-87	0.5077979438946514
1104	88-89	0.5356531465802341
1104	90-91	0.4744254906462295
1104	92-93	0.47015928451234146
1104	94-95	0.43974543509956376
1104	96-97	0.46115856147968515
1104	98-99	0.3240758866603244
1104	100	0.3638536611339198
1105	1	0.39225635934385394
1105	2	0.41018414228425115
1105	3	0.45102016560774416
1105	4	0.3970836276628873
1105	5	0.4363111155907049
1105	6	0.4083342103345444
1105	7	0.3870314877173726
1105	8	0.3584623409865202
1105	9	0.3494639396580226
1105	10-11	0.45756276888007363
1105	12-13	0.497382507865332
1105	14-15	0.6190884017360503
1105	16-17	0.546370490505204
1105	18-19	0.552980729005057
1105	20-21	0.5252777126621098
1105	22-23	0.5867887252533848
1105	24-25	0.5386703500562504
1105	26-27	0.46012306731250163
1105	28-29	0.589237229132074
1105	30-31	0.6505860889261115
1105	32-33	0.5983006098823651
1105	34-35	0.6417506695263953
1105	36-37	0.6388512419320236
1105	38-39	0.5935916407184578
1105	40-41	0.6371483814903485
1105	42-43	0.5741944195766138
1105	44-45	0.5806409422598406
1105	46-47	0.5710716291879194
1105	48-49	0.6366790427891402
1105	50-51	0.5983487529891249
1105	52-53	0.5925905437166037
1105	54-55	0.6431084956492867
1105	56-57	0.610279921021835
1105	58-59	0.6549167873828168
1105	60-61	0.5948609771985574
1105	62-63	0.5603469278887729
1105	64-65	0.522532820817041
1105	66-67	0.5011105296878284
1105	68-69	0.4697806072679782
1105	70-71	0.5051106083237826
1105	72-73	0.46092765461933993
1105	74-75	0.497207484973309
1105	76-77	0.4478836860150395
1105	78-79	0.43711682691400355
1105	80-81	0.47915623816827235
1105	82-83	0.4381096958117361
1105	84-85	0.3876325923546311
1105	86-87	0.42431051016727395
1105	88-89	0.4544639831468409
1105	90-91	0.4569989105026764
1105	92-93	0.42610217093562497
1105	94-95	0.3603435526167118
1105	96-97	0.44173245781833614
1105	98-99	0.4922395961120678
1105	100	0.5665618573840803
1106	1	0.29973473673349105
1106	2	0.3360384645673875
1106	3	0.37644993531860393
1106	4	0.3985393571793878
1106	5	0.3246335119428494
1106	6	0.3310666982802104
1106	7	0.33913921170159256
1106	8	0.37342623500806127
1106	9	0.3813357982858179
1106	10-11	0.3656649850867453
1106	12-13	0.43279000229544806
1106	14-15	0.5084399684406478
1106	16-17	0.5988560662787492
1106	18-19	0.567828240704543
1106	20-21	0.5785067834876187
1106	22-23	0.5969301456161986
1106	24-25	0.5767702470652907
1106	26-27	0.547391010256014
1106	28-29	0.5529586837801475
1106	30-31	0.6014671516847017
1106	32-33	0.606492151105364
1106	34-35	0.5697743287217847
1106	36-37	0.5416259570171178
1106	38-39	0.593427205804268
1106	40-41	0.5597182298933916
1106	42-43	0.5193700026921775
1106	44-45	0.49651817348426874
1106	46-47	0.46666232333349456
1106	48-49	0.43013463881447933
1106	50-51	0.3625847822524122
1106	52-53	0.4239424204924376
1106	54-55	0.4904495292256925
1106	56-57	0.5109887970854388
1106	58-59	0.5379959594672812
1106	60-61	0.5723545159730961
1106	62-63	0.5881442435336837
1106	64-65	0.5370963469775134
1106	66-67	0.5451482485436188
1106	68-69	0.44121286599749254
1106	70-71	0.462822215041939
1106	72-73	0.45834010174036166
1106	74-75	0.5378046382321386
1106	76-77	0.5339844262150564
1106	78-79	0.540227234239449
1106	80-81	0.5074804425652033
1106	82-83	0.47713399796136713
1106	84-85	0.5243003953858967
1106	86-87	0.570665610163136
1106	88-89	0.5702261627363789
1106	90-91	0.6104158405609148
1106	92-93	0.6769909854750082
1106	94-95	0.5313344245251876
1106	96-97	0.5280799031946835
1106	98-99	0.4839420223560893
1106	100	0.5290958321508725
1107	1	0.20803721324418945
1107	2	0.2005533394169916
1107	3	0.2522423615744174
1107	4	0.2525863783451143
1107	5	0.26286335578605247
1107	6	0.24214037311860181
1107	7	0.2687558697800938
1107	8	0.2537160439833457
1107	9	0.2809319904145937
1107	10-11	0.3604418508611076
1107	12-13	0.27819355932309264
1107	14-15	0.33364026168489147
1107	16-17	0.3903771419730262
1107	18-19	0.3757621302326868
1107	20-21	0.3782117568783079
1107	22-23	0.32397406485059577
1107	24-25	0.37075700366123954
1107	26-27	0.38241181890137455
1107	28-29	0.4406963493287037
1107	30-31	0.37287466149985704
1107	32-33	0.26963052889224315
1107	34-35	0.27700580372623307
1107	36-37	0.3418383801998246
1107	38-39	0.35819356064195773
1107	40-41	0.31769367810188953
1107	42-43	0.33498859587475494
1107	44-45	0.3148445324036899
1107	46-47	0.1902960273161014
1107	48-49	0.24493812156443084
1107	50-51	0.28371855326516027
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2312	78-79	-0.34487699620024515
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2312	98-99	0.042221951582998685
2312	100	0.030751917141376595
2313	1	-0.34446697404458604
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2313	32-33	-0.6068149933666618
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2313	80-81	-0.28327099983661697
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2313	86-87	-0.15497638095369481
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2313	100	-0.13571415774456597
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2314	3	-0.5896840718186596
2314	4	-0.4316035527602793
2314	5	-0.53824005956713
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2314	92-93	-0.3269082809035062
2314	94-95	-0.42713567229624516
2314	96-97	-0.4243631877787273
2314	98-99	-0.4105580234293775
2314	100	-0.5261504080060746
2315	1	-0.5526737704003217
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2315	3	-0.6551693840511597
2315	4	-0.5143876329930492
2315	5	-0.534778607246146
2315	6	-0.5091633886234135
2315	7	-0.5313689868924385
2315	8	-0.5049551498508436
2315	9	-0.681574211148785
2315	10-11	-0.6152196040770121
2315	12-13	-0.6881335897320611
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2315	16-17	-0.8225642244111171
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2315	24-25	-0.6955055590333004
2315	26-27	-0.7567212872838809
2315	28-29	-0.7622704237752913
2315	30-31	-0.7815173307365484
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2315	98-99	-0.14078885018451714
2315	100	-0.05849125493708485
2316	1	-0.7804290090517902
2316	2	-0.8506650715342161
2316	3	-0.7619275161243735
2316	4	-0.7540599046333867
2316	5	-0.8058862386465577
2316	6	-0.835120953515414
2316	7	-0.8581534128754242
2316	8	-0.7612867569772064
2316	9	-1.0363947304489898
2316	10-11	-1.0746158965761907
2316	12-13	-0.9739464278255383
2316	14-15	-1.2541729063912044
2316	16-17	-1.3557275354029557
2316	18-19	-1.2631439009016034
2316	20-21	-1.2211762777207653
2316	22-23	-1.2129112844674523
2316	24-25	-1.2843843016062877
2316	26-27	-1.3717248153628816
2316	28-29	-1.2408420071718211
2316	30-31	-1.285848238291905
2316	32-33	-1.4029925108755776
2316	34-35	-1.2497998236367138
2316	36-37	-1.3168447399192615
2316	38-39	-1.2697959054924794
2316	40-41	-1.1723822658561147
2316	42-43	-1.0818349412216648
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2316	46-47	-1.2797335569397674
2316	48-49	-1.08580455420622
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2316	52-53	-0.9432434003730208
2316	54-55	-1.0930858981699174
2316	56-57	-1.0861551009170824
2316	58-59	-1.1795819862737602
2316	60-61	-1.071269967307039
2316	62-63	-1.1165109477256863
2316	64-65	-0.9543273946331894
2316	66-67	-0.917927570397076
2316	68-69	-0.9132472723353828
2316	70-71	-0.8731039300354908
2316	72-73	-0.9017858414285413
2316	74-75	-0.8325961962324797
2316	76-77	-0.8093569883755833
2316	78-79	-0.701303232169483
2316	80-81	-0.597556855178091
2316	82-83	-0.5733997575520142
2316	84-85	-0.7169199212829618
2316	86-87	-0.7971617651120404
2316	88-89	-0.7267412465438632
2316	90-91	-0.6383468915920396
2316	92-93	-0.6428113657570442
2316	94-95	-0.40545797127363414
2316	96-97	-0.6352900886683166
2316	98-99	-0.4738023869828858
2316	100	-0.5436533956767171
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	21314.0
3	3115.0
4	3058.0
5	3448.0
6	4196.0
7	5107.0
8	6516.0
9	8081.0
10	10272.0
11	14960.0
12	22763.0
13	31228.0
14	32017.0
15	37427.0
16	47711.0
17	63297.0
18	62193.0
19	38211.0
20	34561.0
21	33398.0
22	35990.0
23	41600.0
24	46471.0
25	50050.0
26	55408.0
27	64326.0
28	74901.0
29	89874.0
30	108438.0
31	134165.0
32	173203.0
33	233615.0
34	337420.0
35	545219.0
36	989130.0
37	1813103.0
38	1619662.0
39	324045.0
40	327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.554010877256026	17.860345148819228	13.477502999895751	24.108140974029
2	14.707405805892678	21.84577931751612	36.70545197850373	26.741362898087466
3	18.830316978414707	27.17655955513673	29.600279261939477	24.392844204509085
4	13.28845806008678	18.46173316503485	34.397058092149805	33.85275068272856
5	13.511810726013195	36.313290952352034	33.56438652466551	16.610511796969263
6	30.933313058024797	33.71380985765707	18.380348786180082	16.972528298138055
7	25.30781175628175	32.17804415370674	22.273545382511426	20.24059870750008
8	27.42103403564606	31.101361173369924	21.47439200955178	20.003212781432232
9	27.60610354768015	14.751540251964263	19.672227164784406	37.97012903557118
10-11	24.544604515740733	25.72281788629861	26.44581450536868	23.286763092591976
12-13	26.83980372909172	23.719608624479157	26.576227838645767	22.86435980778336
14-15	25.57091569610952	23.083091827118917	25.184071023361078	26.161921453410482
16-17	23.060065995795426	26.293071995236627	27.47198452680771	23.17487748216024
18-19	24.411823737178256	25.45932663521087	28.63316578661336	21.49568384099751
20-21	24.121063245609975	24.995358941059905	28.094795164304593	22.788782649025528
22-23	22.9917685977979	26.138666667406646	25.78295582081691	25.086608913978537
24-25	23.033227901461657	26.93810689767585	25.240177745062486	24.78848745580001
26-27	23.964873880260214	25.075704040638986	25.803011008519377	25.156411070581424
28-29	23.363714235124455	24.35244992655402	28.43257327054893	23.8512625677726
30-31	24.99442794622777	24.50812131176908	27.44071577804271	23.056734963960444
32-33	21.76180494536405	25.759394434905424	27.857568595396636	24.621232024333885
34-35	21.678951542050047	26.226180750143246	28.15556660532725	23.939301102479448
36-37	23.525412392105164	26.062094937052127	26.73850662602981	23.673986044812896
38-39	24.25106566696667	24.561268856607864	26.6196435765975	24.56802189982796
40-41	23.039565582230622	25.75445479538362	26.719260677837692	24.486718944548063
42-43	24.916151573741928	24.970803600432806	26.46317419276107	23.6498706330642
44-45	24.497283670588047	24.515071451748547	27.23588074932814	23.75176412833527
46-47	23.18365688297732	24.945781294444227	28.653769647753457	23.21679217482499
48-49	23.667177225561755	25.83598033518951	27.84183070028042	22.655011738968316
50-51	22.536947693165292	26.34391273967731	26.613360161479676	24.505779405677718
52-53	22.131007246263234	26.779757732937547	25.22958474943364	25.859650271365574
54-55	22.968070888458193	26.05516254559554	25.16571595238448	25.811050613561783
56-57	23.604777448978595	24.535047160074384	25.60959805472797	26.25057733621905
58-59	23.478692607783238	24.311228206910577	27.423962458326578	24.786116726979603
60-61	23.549040204959663	24.985872621313447	26.573561174149845	24.89152599957705
62-63	21.427864539826178	25.40318293722944	27.299374596158227	25.869577926786157
64-65	21.57592966661642	25.31866191738925	27.110380763568326	25.995027652426
66-67	23.20552651737659	25.147992094515253	25.891537270655242	25.754944117452915
68-69	23.66392775837635	24.175245946440228	26.01250429651477	26.14832199866865
70-71	22.547454606300857	24.666097675072052	26.540198715275416	26.24624900335168
72-73	23.70769537346485	24.090473028588608	26.644656916816366	25.557174681130178
74-75	23.745173316517192	23.482044126822704	27.114401002751126	25.658381553908978
76-77	22.614396596512943	24.49360857581724	27.90741170994852	24.984583117721293
78-79	22.880261825834662	24.206530920800308	27.868256594480766	25.044950658884265
80-81	22.24714391673138	24.337740091899075	27.492764686108316	25.92235130526123
82-83	22.351731164144862	25.048335831210544	26.053911148623577	26.546021856021017
84-85	22.594225248430924	24.757212750693423	26.19429265339329	26.454269347482363
86-87	22.854312945862805	23.444493669151452	26.815654474473828	26.885538910511915
88-89	23.015947948409316	22.851016535943636	27.76566739157867	26.36736812406838
90-91	23.545941167007143	23.31567461571425	27.160561317036986	25.97782290024162
92-93	22.641732496668773	23.629905388823044	27.447531245776812	26.28083086873137
94-95	22.293663454753617	23.70915764990055	27.687326687088422	26.30985220825741
96-97	23.547752775602433	23.37550983555772	26.70897824231296	26.367759146526883
98-99	23.813543846580604	22.83242698054692	26.637787435712607	26.71624173715987
100	23.452013277669778	23.160863401916636	27.05338434079185	26.333738979621735
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2523.0
1	3344.5
2	2663.0
3	1044.5
4	833.0
5	889.5
6	1161.0
7	1777.0
8	2634.5
9	2835.0
10	2320.0
11	2003.5
12	2167.5
13	2429.0
14	2694.0
15	3017.0
16	3426.5
17	3858.0
18	4387.5
19	5040.0
20	5882.5
21	6896.5
22	8372.0
23	10330.0
24	12540.0
25	15423.5
26	19722.5
27	25162.0
28	30668.5
29	38173.0
30	47439.5
31	56574.0
32	66420.5
33	76539.5
34	87345.5
35	95451.5
36	101559.0
37	110041.5
38	115977.0
39	119890.5
40	124370.5
41	128987.0
42	136342.0
43	152169.5
44	171255.5
45	188645.5
46	218321.0
47	261911.0
48	381156.0
49	520288.0
50	633182.0
51	583582.0
52	397606.5
53	308639.5
54	265171.5
55	231268.0
56	209268.0
57	188543.0
58	175639.5
59	159883.0
60	136273.0
61	113923.0
62	93672.0
63	73414.0
64	54274.0
65	43459.0
66	34131.0
67	25077.0
68	19782.5
69	16350.5
70	13585.0
71	11437.0
72	9489.0
73	9241.0
74	7736.0
75	6166.0
76	4782.0
77	3103.5
78	2128.0
79	1390.0
80	1008.0
81	802.5
82	581.5
83	410.0
84	292.0
85	197.5
86	151.5
87	108.5
88	79.5
89	64.0
90	47.5
91	43.0
92	42.0
93	39.5
94	32.5
95	23.5
96	18.5
97	16.5
98	12.5
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08637334448781271
2	0.28556113587319354
3	0.2887052585798538
4	0.29776365615763273
5	0.3234568174829843
6	0.17129235908928478
7	0.18878587000783953
8	0.15392350501813065
9	0.2054067829945899
10-11	0.2261621480867944
12-13	0.24280383721477822
14-15	0.1700319398544562
16-17	0.15903443576155638
18-19	0.17695039488519104
20-21	0.20923651836195364
22-23	0.17204030017368854
24-25	0.19694396813216952
26-27	0.1956835488973409
28-29	0.22474937048292065
30-31	0.25907848118097127
32-33	0.2266884769980415
34-35	0.1526838619245355
36-37	0.14683191547711716
38-39	0.11435880672925364
40-41	0.10515497616284063
42-43	0.12109027648888754
44-45	0.13593829209038452
46-47	0.20099531567268988
48-49	0.20735281489012192
50-51	0.21393885166112175
52-53	0.14090378984517618
54-55	0.08478050699324914
56-57	0.12327869669880966
58-59	0.14319609076126552
60-61	0.11973982730871408
62-63	0.14070295381325296
64-65	0.11547379297544814
66-67	0.11944896133144593
68-69	0.10497491627215083
70-71	0.13023870401201137
72-73	0.155080043546792
74-75	0.1945200849882684
76-77	0.17484507924020268
78-79	0.19972104567703902
80-81	0.1032089442673086
82-83	0.14356313592305625
84-85	0.17501128837007016
86-87	0.1888897507140067
88-89	0.17006656675651194
90-91	0.1734184508755066
92-93	0.12307093528647528
94-95	0.3626683213708929
96-97	0.362910709685283
98-99	0.41063350609849003
100	0.44478948228626203
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7219820.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.45839454140302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.96702083869253	31.15911864012324
2	11.6180390913515	9.168583405279819
3	3.555300326781235	4.208593290219391
4	1.6144269491423657	2.548107820701322
5	0.9433309546813118	1.8611162496466784
6	0.6092680636682518	1.4424443782619119
7	0.42841571792203564	1.1833217497854276
8	0.3010661389967614	0.9503669196472873
9	0.241640266854413	0.8581263287968204
>10	1.45930650106253	10.881722717264607
>50	0.1319847915464855	3.6351278049292572
>100	0.10611114760464217	8.528526925184002
>500	0.013740058685521927	3.6742154583606217
>1k	0.008477121919812574	6.184605873473997
>5k	8.477121919538299E-4	2.38891163145566
>10k+	0.001024318898610878	11.327110806869918
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	101097	1.400270366851251	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	89453	1.2389921078364836	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	54755	0.7583984088245967	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	44234	0.6126745542132629	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	42544	0.5892667684235895	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	41799	0.578947951610982	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	41163	0.5701388677280043	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	39144	0.5421741816277967	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	34920	0.4836685679144356	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	33412	0.4627816205944192	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	28835	0.39938668831078894	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	27103	0.37539717056657923	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	18304	0.2535243260912322	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18141	0.2512666520771986	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	18111	0.25085112925252984	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	17651	0.24447977927427555	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	17226	0.23859320592480143	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	13666	0.18928449739744205	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	13542	0.18756700305547783	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	13039	0.18060007036186498	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12902	0.17870251612921098	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	12544	0.17374394375483046	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	12504	0.17318991332193878	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	11999	0.16619527910668133	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	11311	0.15666595566094446	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	11291	0.1563889404444986	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	10952	0.15169353252574164	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	10762	0.14906188796950615	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	10319	0.14292600092523083	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	9896	0.13706712909740132	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	9291	0.1286874187999147	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	9132	0.12648514782917025	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9040	0.1252108778335194	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	9015	0.1248646088129621	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	9007	0.12475380272638376	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	8726	0.12086173893531972	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	7605	0.10533503605353042	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1080608657833575E-4	0.0	0.0	2.7701521644583938E-5	0.0
2	2.216121731566715E-4	0.0	0.0	4.15522824668759E-5	0.0
3	2.3546293397896345E-4	0.0	0.0	4.15522824668759E-5	0.0
4	3.324182597350072E-4	0.0	0.0	8.31045649337518E-5	0.0
5	0.0012604192348285692	0.0	0.0	1.2465684740062773E-4	0.0
6	0.0025485399913017224	0.0	0.0	1.3850760822291968E-4	1.3850760822291969E-5
7	0.0028394059685698534	0.0	0.0	1.3850760822291968E-4	1.3850760822291969E-5
8	0.0032549287932386126	0.0	0.0	1.800598906897956E-4	1.3850760822291969E-5
9	0.003545794770506744	0.0	0.0	2.0776141233437952E-4	2.7701521644583938E-5
10-11	0.005893498729885233	0.0	0.0	3.047167380904233E-4	2.7701521644583938E-5
12-13	0.012257923327728393	6.9253804111459844E-6	0.0	3.947466834353211E-4	3.462690205572992E-5
14-15	0.019640378846010012	2.0776141233437955E-5	0.0	5.817319545362627E-4	5.5403043289167875E-5
16-17	0.02239668024964611	2.7701521644583938E-5	0.0	8.033441276929341E-4	6.925380411145984E-5
18-19	0.023934114700920522	2.7701521644583938E-5	0.0	0.0011149862461945035	9.695532575604379E-5
20-21	0.029398239845314703	2.7701521644583938E-5	0.0	0.0019945095584100432	9.695532575604379E-5
22-23	0.031420450925369325	2.7701521644583938E-5	0.0	0.005914274871118671	1.0388070616718976E-4
24-25	0.043976165610776996	2.7701521644583938E-5	0.0	0.014377089733539064	1.1080608657833575E-4
26-27	0.0824397284142818	2.7701521644583938E-5	0.0	0.030499375330686918	1.2465684740062773E-4
28-29	0.09142194680753814	2.7701521644583938E-5	0.0	0.09300093354127942	1.3158222781177372E-4
30-31	0.09335412794224787	2.7701521644583938E-5	0.0	0.2447013914474322	1.3850760822291968E-4
32-33	0.09472535326365478	2.7701521644583938E-5	0.0	0.4833707765567562	1.3850760822291968E-4
34-35	0.0957987872273824	2.7701521644583938E-5	0.0	0.7973536736372929	1.5235836904521166E-4
36-37	0.09795950591565994	2.7701521644583938E-5	0.0	1.2309101888966762	1.5235836904521166E-4
38-39	0.09999556775653687	2.7701521644583938E-5	0.0	1.8315484319553672	1.5928374945635762E-4
40-41	0.1020039280757692	2.7701521644583938E-5	0.0	2.6639168289514146	1.662091298675036E-4
42-43	0.10678936593987107	2.7701521644583938E-5	0.0	3.2563831231249534	1.662091298675036E-4
44-45	0.11219116266056495	2.7701521644583938E-5	0.0	3.8661282414243017	1.662091298675036E-4
46-47	0.1144280605333651	2.7701521644583938E-5	0.0	4.547751883010934	1.800598906897956E-4
48-49	0.11592394270217263	2.7701521644583938E-5	0.0	5.143438479075655	1.9391065151208757E-4
50-51	0.11789767611934923	2.7701521644583938E-5	0.0	5.772283796548944	1.9391065151208757E-4
52-53	0.11885337861608739	2.7701521644583938E-5	0.0	6.526589305550553	2.493136948012554E-4
54-55	0.12160275463931233	2.7701521644583938E-5	0.0	7.184943668955736	2.977913576792773E-4
56-57	0.1277247909227654	2.7701521644583938E-5	0.0	7.8552720150917885	3.047167380904233E-4
58-59	0.1298508827089872	2.7701521644583938E-5	0.0	8.531417680773206	3.1164211850156934E-4
60-61	0.1310697496613489	2.7701521644583938E-5	0.0	9.11800432697768	3.185674989127153E-4
62-63	0.13222628819001025	2.7701521644583938E-5	0.0	9.707187990836337	3.462690205572992E-4
64-65	0.13304348307852548	4.15522824668759E-5	0.0	10.353035117218989	3.462690205572992E-4
66-67	0.13417924546595345	4.15522824668759E-5	0.0	11.001022186148685	3.462690205572992E-4
68-69	0.13550199312448233	4.15522824668759E-5	0.0	11.757502264599395	3.462690205572992E-4
70-71	0.1369009199675338	5.5403043289167875E-5	0.0	12.649795978293088	3.531944009684452E-4
72-73	0.1409938197905211	5.5403043289167875E-5	0.0	13.36847040507935	4.0167206384646707E-4
74-75	0.14493436124446316	6.232842370031385E-5	0.0	14.055385037300098	4.0167206384646707E-4
76-77	0.14603549672983537	6.925380411145984E-5	0.0	14.779163469449376	4.0167206384646707E-4
78-79	0.14701197536780697	6.925380411145984E-5	0.0	15.466500549875203	4.0167206384646707E-4
80-81	0.14878487275306032	6.925380411145984E-5	0.0	16.223777047073195	4.2244820507990506E-4
82-83	0.14958821688075324	8.31045649337518E-5	0.0	17.103612001407235	4.57075107135635E-4
84-85	0.15224756295863331	8.31045649337518E-5	0.0	17.897523760980192	4.57075107135635E-4
86-87	0.15762165815768261	8.31045649337518E-5	1.3850760822291969E-5	18.658436082894035	4.57075107135635E-4
88	0.15895133119662264	8.31045649337518E-5	1.3850760822291969E-5	19.256408054494432	4.57075107135635E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTACA	7795	0.0	28.545897	2
TCTACAC	9055	0.0	26.803251	3
TACACTC	9155	0.0	26.102547	5
CTACACT	10720	0.0	23.34551	4
CACTCTT	11150	0.0	20.995552	7
ACACTCT	11820	0.0	19.924759	6
TATCACG	380	0.0	19.807743	2
AGTACTC	3080	0.0	18.785896	5
ACTCTTT	13950	0.0	18.60106	8
AGTACTT	25990	0.0	17.930023	12-13
GAGTACT	25450	0.0	17.470196	12-13
GTATAAG	1815	0.0	16.6003	1
GTACTTT	28580	0.0	15.610566	14-15
TATACCG	855	0.0	15.405275	5
GCCGTAT	6455	0.0	15.393943	94
TACTTTT	30985	0.0	15.210213	14-15
CATGGGG	12725	0.0	15.156978	4
GTATATA	1480	0.0	14.950261	1
ATACCGA	795	0.0	14.782426	6
TAACGCA	485	4.838512E-10	14.548797	5
>>END_MODULE