##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765442_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6140277
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61195740843613	34.0	31.0	34.0	31.0	34.0
2	32.650910699957024	34.0	31.0	34.0	31.0	34.0
3	32.695277428037855	34.0	31.0	34.0	31.0	34.0
4	36.1254874983653	37.0	37.0	37.0	35.0	37.0
5	36.133203274054246	37.0	37.0	37.0	35.0	37.0
6	36.253319027789786	37.0	37.0	37.0	35.0	37.0
7	36.21717098430576	37.0	37.0	37.0	35.0	37.0
8	36.254744207793884	37.0	37.0	37.0	35.0	37.0
9	38.01889833308823	39.0	39.0	39.0	37.0	39.0
10-11	37.96781586563603	39.0	38.5	39.0	37.0	39.0
12-13	37.95659487348861	39.0	39.0	39.0	37.0	39.0
14-15	39.41129870851103	41.0	39.5	41.0	37.0	41.0
16-17	39.349382039279334	41.0	39.0	41.0	37.0	41.0
18-19	39.33928583352184	41.0	39.0	41.0	37.0	41.0
20-21	39.32843518297301	41.0	39.0	41.0	37.0	41.0
22-23	39.29533854905894	41.0	39.0	41.0	36.5	41.0
24-25	39.25358807102676	41.0	39.0	41.0	36.0	41.0
26-27	39.12658964734001	41.0	39.0	41.0	36.0	41.0
28-29	38.98931090568064	41.0	39.0	41.0	35.5	41.0
30-31	38.76398572898258	40.0	38.5	41.0	35.0	41.0
32-33	38.457602319895344	40.0	38.0	41.0	35.0	41.0
34-35	38.38766614274894	40.0	38.0	41.0	35.0	41.0
36-37	38.30879022233036	40.0	38.0	41.0	35.0	41.0
38-39	38.13367621688728	40.0	38.0	41.0	34.5	41.0
40-41	37.90696234062405	40.0	38.0	41.0	34.0	41.0
42-43	37.666813321288274	40.0	38.0	41.0	33.0	41.0
44-45	37.283464003334046	40.0	37.0	41.0	33.0	41.0
46-47	36.89846638840561	40.0	37.0	41.0	32.0	41.0
48-49	36.5769177025727	40.0	36.5	41.0	31.0	41.0
50-51	35.394585211709504	38.5	35.0	40.0	29.5	40.5
52-53	35.38116855314507	38.5	35.0	39.5	29.5	40.5
54-55	36.22523959423981	39.5	36.0	40.5	31.0	41.0
56-57	36.23772820020986	40.0	36.0	41.0	31.0	41.0
58-59	36.06062096221392	39.0	35.0	41.0	31.0	41.0
60-61	35.67581560245572	39.0	35.0	41.0	30.0	41.0
62-63	35.24341059857723	38.0	35.0	40.5	28.5	41.0
64-65	35.022341500228734	38.0	35.0	40.0	29.0	41.0
66-67	34.665865074165225	37.0	35.0	40.0	28.5	41.0
68-69	34.3925752046691	37.0	35.0	39.5	28.0	41.0
70-71	33.94603248355082	36.0	35.0	39.0	27.5	41.0
72-73	33.459555000531736	35.0	34.5	39.0	26.5	39.5
74-75	33.00703706038017	35.0	34.0	37.0	26.0	39.0
76-77	32.54415900129587	35.0	34.0	37.0	25.0	39.0
78-79	32.205108824895035	35.0	34.0	36.0	24.0	38.0
80-81	31.882744947825643	35.0	34.0	36.0	23.5	37.0
82-83	31.61761106217195	35.0	34.0	35.5	21.0	37.0
84-85	31.344705947304984	35.0	33.5	35.0	20.0	36.0
86-87	31.053991538166763	35.0	33.0	35.0	18.0	36.0
88-89	30.79162609439281	35.0	33.0	35.0	12.5	36.0
90-91	30.544698227783535	35.0	33.0	35.0	4.5	35.5
92-93	30.226960933521404	35.0	32.0	35.0	2.0	35.0
94-95	29.931298376278463	35.0	32.0	35.0	2.0	35.0
96-97	29.628053994958208	35.0	32.0	35.0	2.0	35.0
98-99	29.272751213015308	35.0	31.5	35.0	2.0	35.0
100	28.664280617959093	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.38097194948106505
1101	2	0.41667574738064417
1101	3	0.4267279028941928
1101	4	0.33623925021524315
1101	5	0.3395831483150076
1101	6	0.2995517563081407
1101	7	0.3196620312471907
1101	8	0.23838296408266757
1101	9	0.3230920218367075
1101	10-11	0.3033081299161964
1101	12-13	0.31717265505745473
1101	14-15	0.4934721344259003
1101	16-17	0.4708777133679334
1101	18-19	0.46264994396221937
1101	20-21	0.4658566646245177
1101	22-23	0.5215923780095792
1101	24-25	0.5013891048723522
1101	26-27	0.5940067970060525
1101	28-29	0.5961974878322351
1101	30-31	0.5580228505914135
1101	32-33	0.5134885661262558
1101	34-35	0.5578853408927458
1101	36-37	0.5865078905504078
1101	38-39	0.617175859454612
1101	40-41	0.6355246298477653
1101	42-43	0.5362331399004034
1101	44-45	0.6288493027982298
1101	46-47	0.7037778873023086
1101	48-49	0.570302097822676
1101	50-51	0.6378208703973911
1101	52-53	0.6493063649967254
1101	54-55	0.7759406971450531
1101	56-57	0.8682216957350235
1101	58-59	0.8413136481606855
1101	60-61	0.8853619208890677
1101	62-63	0.8615392760792915
1101	64-65	0.8482271604689089
1101	66-67	0.7778015688523041
1101	68-69	0.7382701062279651
1101	70-71	0.7049881621481049
1101	72-73	0.7409509446756815
1101	74-75	0.7140800041085811
1101	76-77	0.6500865714449873
1101	78-79	0.6364558684875448
1101	80-81	0.6188706740360921
1101	82-83	0.7065353672416812
1101	84-85	0.704280667594336
1101	86-87	0.674920783950256
1101	88-89	0.6709967393460055
1101	90-91	0.7021528083084192
1101	92-93	0.6511111977838375
1101	94-95	0.6188462414386215
1101	96-97	0.6789265265347666
1101	98-99	0.6624547579274989
1101	100	0.716728020198655
1102	1	0.37167241920710126
1102	2	0.4304153875852563
1102	3	0.44714023504706546
1102	4	0.35931146751886445
1102	5	0.34270215377465263
1102	6	0.3113319503467977
1102	7	0.3148916067722496
1102	8	0.296794226440646
1102	9	0.23185450277713926
1102	10-11	0.3436563051166317
1102	12-13	0.34779192181757423
1102	14-15	0.4748474818018238
1102	16-17	0.5127954590518655
1102	18-19	0.5258181815058762
1102	20-21	0.43045483060258505
1102	22-23	0.48650279805777785
1102	24-25	0.4795952617264163
1102	26-27	0.4392286103698879
1102	28-29	0.3741918375936777
1102	30-31	0.370231807971777
1102	32-33	0.4184668781866563
1102	34-35	0.44685830705279983
1102	36-37	0.49669253776402655
1102	38-39	0.47509287964545877
1102	40-41	0.4559119847491431
1102	42-43	0.4703008812805649
1102	44-45	0.3983567667695027
1102	46-47	0.40327788115406094
1102	48-49	0.39415767858604767
1102	50-51	0.38836783377843886
1102	52-53	0.3998400327946712
1102	54-55	0.5193160383727289
1102	56-57	0.5595695758196229
1102	58-59	0.5586992754047984
1102	60-61	0.5898612845455631
1102	62-63	0.5292599059056684
1102	64-65	0.5230121255468703
1102	66-67	0.4950355649666065
1102	68-69	0.5164047897162334
1102	70-71	0.4660909731264127
1102	72-73	0.37605627950918574
1102	74-75	0.32398667372640233
1102	76-77	0.38450629993842256
1102	78-79	0.39944926527078906
1102	80-81	0.3914880748033944
1102	82-83	0.46327123242292956
1102	84-85	0.46263259937339996
1102	86-87	0.48765434046493894
1102	88-89	0.518496478045531
1102	90-91	0.5013953443371442
1102	92-93	0.5390291248410399
1102	94-95	0.5530892846976876
1102	96-97	0.559672908291688
1102	98-99	0.5281819725258927
1102	100	0.5372943659268508
1103	1	0.3850417916427986
1103	2	0.44231318263587127
1103	3	0.45332190199157907
1103	4	0.3146269746363828
1103	5	0.34671530984144283
1103	6	0.33145460253762593
1103	7	0.3303891154086642
1103	8	0.28913823258107385
1103	9	0.3434611081321819
1103	10-11	0.37313213472217654
1103	12-13	0.38167488619292556
1103	14-15	0.46328433349160036
1103	16-17	0.4951445619525927
1103	18-19	0.4887107420302641
1103	20-21	0.46705375174490626
1103	22-23	0.49391987223373235
1103	24-25	0.442289494312341
1103	26-27	0.41743200091659816
1103	28-29	0.44096388361444383
1103	30-31	0.5189756634958371
1103	32-33	0.520339930361736
1103	34-35	0.5183586763580834
1103	36-37	0.49626802480707966
1103	38-39	0.4364902747118151
1103	40-41	0.45398275533531063
1103	42-43	0.4578964491540205
1103	44-45	0.5145435102146223
1103	46-47	0.5357460387027047
1103	48-49	0.5216595337952157
1103	50-51	0.5101616647593374
1103	52-53	0.4597137975321459
1103	54-55	0.5693752111520354
1103	56-57	0.5614517014436231
1103	58-59	0.5748178160353277
1103	60-61	0.5430715923308327
1103	62-63	0.5401464527738682
1103	64-65	0.502908285929557
1103	66-67	0.520100714355813
1103	68-69	0.4785854390452329
1103	70-71	0.445619709477171
1103	72-73	0.3805618185096762
1103	74-75	0.4384614729817784
1103	76-77	0.4037281192821496
1103	78-79	0.36492414093414993
1103	80-81	0.35697467214717093
1103	82-83	0.43158550427792974
1103	84-85	0.5424831465009241
1103	86-87	0.4114948926168651
1103	88-89	0.38988323387800605
1103	90-91	0.42986820329262443
1103	92-93	0.3856746693290134
1103	94-95	0.3686039996032626
1103	96-97	0.457918809466058
1103	98-99	0.48886195733691196
1103	100	0.4945427926168584
1104	1	0.2601956344513283
1104	2	0.33081628667275
1104	3	0.36030951846091597
1104	4	0.33117472570458517
1104	5	0.32593484298560327
1104	6	0.28319688593455794
1104	7	0.2905826964126135
1104	8	0.236100788437426
1104	9	0.3104642719666231
1104	10-11	0.3317064929856528
1104	12-13	0.328849859284027
1104	14-15	0.4154884171211961
1104	16-17	0.36467498803737897
1104	18-19	0.36661343781290867
1104	20-21	0.3862309645263622
1104	22-23	0.3646462494802236
1104	24-25	0.38769208137214406
1104	26-27	0.3645716390870106
1104	28-29	0.4164137243354915
1104	30-31	0.37619581092400267
1104	32-33	0.42505822950173666
1104	34-35	0.39087836878119475
1104	36-37	0.3857684569803368
1104	38-39	0.4366912760491033
1104	40-41	0.37724434518929684
1104	42-43	0.3249875425795494
1104	44-45	0.34379423322145897
1104	46-47	0.302474405577037
1104	48-49	0.2794326047803537
1104	50-51	0.16566168106776047
1104	52-53	0.2195998288225809
1104	54-55	0.349552048839719
1104	56-57	0.33260366062906854
1104	58-59	0.33224412913533996
1104	60-61	0.38894793913492975
1104	62-63	0.35618388604522977
1104	64-65	0.44418932629834273
1104	66-67	0.3975197973874387
1104	68-69	0.37576469309787797
1104	70-71	0.3379508765080601
1104	72-73	0.29767044349200944
1104	74-75	0.2802165449019327
1104	76-77	0.2082784416453478
1104	78-79	0.2617882346749738
1104	80-81	0.2834013195699008
1104	82-83	0.30647943156410307
1104	84-85	0.31452303147216654
1104	86-87	0.1936502140559213
1104	88-89	0.17157702689148024
1104	90-91	0.13503396484170693
1104	92-93	0.14151335726186787
1104	94-95	0.10591319103155783
1104	96-97	0.11536013990736294
1104	98-99	0.14488554203743575
1104	100	0.1844109118205708
1105	1	0.2183811748757023
1105	2	0.2640387753243658
1105	3	0.25192679515035366
1105	4	0.24708791245148376
1105	5	0.24010599622271656
1105	6	0.23101618648199462
1105	7	0.1832394247602025
1105	8	0.22053842133793466
1105	9	0.22576837849815945
1105	10-11	0.28440531592070073
1105	12-13	0.2756887122300853
1105	14-15	0.33017948469875336
1105	16-17	0.35996258097348033
1105	18-19	0.33484465454065315
1105	20-21	0.31040773540211575
1105	22-23	0.3725810697911882
1105	24-25	0.3413428866926935
1105	26-27	0.30940096089852176
1105	28-29	0.34190988624413166
1105	30-31	0.351389412094278
1105	32-33	0.359694930402064
1105	34-35	0.37990672466048636
1105	36-37	0.38320649329516954
1105	38-39	0.34656154252876803
1105	40-41	0.3316527776796079
1105	42-43	0.3210467125263108
1105	44-45	0.275560377334628
1105	46-47	0.22955255484787074
1105	48-49	0.41186494805200624
1105	50-51	0.3495247313324299
1105	52-53	0.25664589345936406
1105	54-55	0.31861695707238624
1105	56-57	0.35990736374629506
1105	58-59	0.3297071432058303
1105	60-61	0.3426081700236452
1105	62-63	0.3747432068010852
1105	64-65	0.3243959159015688
1105	66-67	0.30274193917814074
1105	68-69	0.3118471819003048
1105	70-71	0.29500544300732656
1105	72-73	0.2544647700540139
1105	74-75	0.3486547471067567
1105	76-77	0.34499065265742956
1105	78-79	0.3099940777731973
1105	80-81	0.2426275369762827
1105	82-83	0.2072844693417757
1105	84-85	0.20163281294979285
1105	86-87	0.23791117410659268
1105	88-89	0.25443606563433363
1105	90-91	0.34361377678277094
1105	92-93	0.2960938063713421
1105	94-95	0.2618020975148383
1105	96-97	0.19111823835553565
1105	98-99	0.1894080616049152
1105	100	0.24869705698940336
1106	1	0.1475728841136501
1106	2	0.19067646993124754
1106	3	0.21187196142079046
1106	4	0.20303386855130157
1106	5	0.17779754513578894
1106	6	0.21187392021339235
1106	7	0.19105270699925825
1106	8	0.20956389945119014
1106	9	0.22874631482428498
1106	10-11	0.2116514150460418
1106	12-13	0.24270695781284957
1106	14-15	0.2692119961377415
1106	16-17	0.27541222017697464
1106	18-19	0.28770162640122976
1106	20-21	0.24872092546529245
1106	22-23	0.2917316362578646
1106	24-25	0.3295862240363334
1106	26-27	0.35517279631695686
1106	28-29	0.3076131183412585
1106	30-31	0.30911161058084247
1106	32-33	0.3245845683474258
1106	34-35	0.27009226466700653
1106	36-37	0.2729784650115832
1106	38-39	0.31743042473672745
1106	40-41	0.2854159127430549
1106	42-43	0.38688611155701835
1106	44-45	0.29954410545159504
1106	46-47	0.31129508969341657
1106	48-49	0.2809761104204753
1106	50-51	0.2181741842620042
1106	52-53	0.24213797725466435
1106	54-55	0.2393824799717592
1106	56-57	0.2942546754868758
1106	58-59	0.22889564594620282
1106	60-61	0.2592152063199791
1106	62-63	0.3001823528068073
1106	64-65	0.3352397315113578
1106	66-67	0.2881734315275466
1106	68-69	0.22646811185449423
1106	70-71	0.2400501810749205
1106	72-73	0.17393353990350136
1106	74-75	0.26284874108343814
1106	76-77	0.2881108805895991
1106	78-79	0.2926138561841185
1106	80-81	0.2698725439702976
1106	82-83	0.2904518423989835
1106	84-85	0.25674595338652395
1106	86-87	0.2865845403436573
1106	88-89	0.31428136741643087
1106	90-91	0.3089139250505184
1106	92-93	0.3180192164229645
1106	94-95	0.28780591910076936
1106	96-97	0.3070612588063497
1106	98-99	0.32217239451799884
1106	100	0.31929391112696237
1107	1	0.2128433854818752
1107	2	0.21576818664173203
1107	3	0.2775823189174105
1107	4	0.23877551912424622
1107	5	0.24936493279284377
1107	6	0.21783731363771608
1107	7	0.2470136930528355
1107	8	0.23334850300523868
1107	9	0.21044722445378028
1107	10-11	0.2657799872996023
1107	12-13	0.22653858945741234
1107	14-15	0.2844622989109098
1107	16-17	0.3346489313875409
1107	18-19	0.33203704187021543
1107	20-21	0.3345574374196332
1107	22-23	0.3107800156309608
1107	24-25	0.3259916422803073
1107	26-27	0.37278566942683966
1107	28-29	0.36125371448581944
1107	30-31	0.36982812093302186
1107	32-33	0.39194302146288607
1107	34-35	0.41880783292103985
1107	36-37	0.39544616131964716
1107	38-39	0.4509338515021071
1107	40-41	0.4326815990954245
1107	42-43	0.4201444803253409
1107	44-45	0.3884095424799199
1107	46-47	0.43605498703928447
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2316	54-55	-0.58376072415588
2316	56-57	-0.6005880728967767
2316	58-59	-0.610931818620081
2316	60-61	-0.5298395263693934
2316	62-63	-0.5831411866013028
2316	64-65	-0.5016653669414097
2316	66-67	-0.4920536745987292
2316	68-69	-0.4727319273917914
2316	70-71	-0.3673780965106914
2316	72-73	-0.39454632643324317
2316	74-75	-0.30630558259043283
2316	76-77	-0.3205897955265229
2316	78-79	-0.2883324240477876
2316	80-81	-0.27670161157277207
2316	82-83	-0.23198884611925408
2316	84-85	-0.3127415755055587
2316	86-87	-0.28983846405143154
2316	88-89	-0.311141933090493
2316	90-91	-0.18870359234649214
2316	92-93	-0.1337473782804075
2316	94-95	0.02895607532711253
2316	96-97	-0.16351613102610685
2316	98-99	0.033930575269032204
2316	100	-0.06568351343422307
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	15114.0
3	1748.0
4	1603.0
5	1681.0
6	2013.0
7	2302.0
8	2939.0
9	3540.0
10	5047.0
11	8558.0
12	15619.0
13	24361.0
14	24104.0
15	28159.0
16	37515.0
17	52263.0
18	52708.0
19	29926.0
20	25323.0
21	22442.0
22	22713.0
23	25957.0
24	29142.0
25	30399.0
26	33085.0
27	38815.0
28	46595.0
29	56812.0
30	70337.0
31	90902.0
32	122138.0
33	172412.0
34	259609.0
35	426191.0
36	806160.0
37	1672677.0
38	1544325.0
39	334383.0
40	660.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.59802010085307	17.20293281688704	13.424104789656905	23.774942292602987
2	12.8058882872738	22.42933653565726	37.159689125499725	27.605086051569216
3	19.630473108600924	28.69512194325362	29.393755507320122	22.280649440825336
4	12.99438986517516	19.067713569562603	34.2000364515048	33.73786011375744
5	12.349995940703893	36.93944755348592	34.08962566429115	16.620930841519037
6	30.027266819432192	32.23678286764207	19.55159490340799	18.184355409517742
7	23.615131906843377	32.6916102558882	22.744519169386667	20.948738667881756
8	27.73413832240412	31.22864180601292	22.363563208511426	18.67365666307153
9	28.19227362709015	15.02252747647228	19.755565621432662	37.02963327500491
10-11	23.803426213836644	26.9395049301754	26.053309625296876	23.20375923069108
12-13	27.19798457515738	24.338199035608636	26.210161644345874	22.253654744888106
14-15	26.53525841994309	22.393706353934157	25.658251018992708	25.412784207130045
16-17	22.799796873525715	26.151359233997574	29.190778149404302	21.858065743072412
18-19	24.8237050894333	25.418978237453814	29.81129034547098	19.946026327641903
20-21	23.02286054995991	25.354604663488228	29.65942515910419	21.963109627447672
22-23	21.604847397778748	27.452650610732675	25.37348515760918	25.569016833879388
24-25	22.108626938283777	28.228651039823504	24.552627813457477	25.110094208435246
26-27	23.82424045895715	24.90883468038807	25.238874306842956	26.028050553811827
28-29	23.068421565855434	24.084657198566934	29.176680061379855	23.670241174197777
30-31	25.651729292277594	24.434265147918598	27.783575135387167	22.13043042441664
32-33	20.313878569817508	26.258691875049166	29.04880702250299	24.378622532630335
34-35	19.648116822625006	27.099386908893543	29.400248093794197	23.852248174687258
36-37	23.22739271497307	26.597681281519197	27.021221963220572	23.153704040287167
38-39	24.654349386783952	24.4907691320709	26.66812201764795	24.186759463497197
40-41	22.524307582257226	26.453370775572587	26.58891305104448	24.433408591125712
42-43	25.425144807332796	24.99515759974774	26.289733361463817	23.289964231455645
44-45	25.376297077442544	23.913783124093914	27.673716795392277	23.036203003071265
46-47	23.119553924030185	24.759557823809132	30.361753448306107	21.75913480385458
48-49	23.640818633637263	25.931562744865754	29.3387396754563	21.088878946040683
50-51	21.573617707972375	26.831384919711827	27.682540827423935	23.912456544891867
52-53	20.861551336742256	28.033269406181965	24.703809317328634	26.401369939747145
54-55	22.139572007038467	27.057867845568396	24.369823914484854	26.432736232908283
56-57	23.59790507009527	24.35365389470746	25.15418366808716	26.894257367110107
58-59	23.298544988590745	24.12932311331428	28.341766439052314	24.230365459042666
60-61	23.747661077600334	25.149991436281162	26.840056226255655	24.26229125986285
62-63	19.86267442818994	25.91851183312133	28.440952539909926	25.77786119877881
64-65	20.010947799863132	26.127629499714892	27.945534899474662	25.915887800947313
66-67	23.227866670503587	25.56486896360684	25.740318386654433	25.466945979235145
68-69	24.205436428393504	24.16040238347064	25.866968534692436	25.76719265344342
70-71	22.555721094745795	25.258107158190334	26.246167954355755	25.940003792708122
72-73	24.704851587277187	24.128495378149513	26.08728448618574	25.07936854838756
74-75	24.974341792853	23.145730050942788	26.927204437888864	24.952723718315347
76-77	22.88903392360578	24.8870983028165	28.824184591603753	23.399683181973966
78-79	23.429884278043684	24.65898493711281	28.326903248012297	23.58422753683121
80-81	21.94840847713466	25.336169923498954	27.511612661173075	25.203808938193312
82-83	21.963294474194022	26.365946814096077	25.01916375184391	26.651594959865992
84-85	22.77058556579006	26.005563891278683	24.916862988443196	26.30698755448806
86-87	23.358820023171894	23.637656064202606	25.895524829190286	27.107999083435214
88-89	23.59956196079554	22.88205132973216	27.818213567030366	25.700173142441933
90-91	24.393904409125362	23.82749446179025	26.83088797886521	24.947713150219183
92-93	22.251976366108146	24.45182192862288	27.660035681059203	25.63616602420977
94-95	21.800465477164753	24.689090865116864	28.00541139265329	25.505032265065093
96-97	24.108924316324423	24.131769949054075	26.102655167480304	25.6566505671412
98-99	25.06636738976024	23.219263305011403	25.754029599017937	25.960339706210416
100	24.44925992040291	23.813170072343894	26.62404152721094	25.11352848004226
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2002.0
1	2656.5
2	2136.0
3	847.0
4	664.5
5	666.5
6	860.0
7	1387.0
8	2081.0
9	2246.5
10	1807.5
11	1604.0
12	1816.0
13	2009.0
14	2223.5
15	2558.0
16	2964.0
17	3393.5
18	3901.0
19	4380.5
20	5165.5
21	6387.5
22	7794.5
23	9460.0
24	11540.5
25	14355.0
26	18497.0
27	23344.5
28	28271.5
29	35079.5
30	42154.5
31	49054.5
32	56957.0
33	65430.0
34	73778.5
35	79763.5
36	86373.0
37	93295.5
38	96154.5
39	98057.5
40	100972.0
41	103449.0
42	106448.5
43	113731.5
44	123890.5
45	135071.5
46	157506.0
47	196047.0
48	336164.5
49	515632.0
50	729758.5
51	696790.0
52	405752.5
53	272558.5
54	211111.5
55	159750.0
56	133528.5
57	115988.0
58	105091.0
59	94550.5
60	79586.0
61	64654.5
62	51754.5
63	40096.5
64	29309.5
65	22968.0
66	18550.0
67	14289.5
68	11151.5
69	9078.5
70	7668.0
71	6407.5
72	5428.0
73	5363.5
74	4303.5
75	3169.5
76	2528.5
77	1885.5
78	1400.5
79	1056.5
80	839.5
81	677.5
82	571.5
83	491.0
84	352.5
85	199.5
86	126.5
87	97.0
88	71.5
89	46.0
90	44.5
91	46.5
92	42.0
93	29.5
94	19.5
95	17.0
96	11.0
97	12.5
98	13.0
99	8.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0816738397958268
2	0.26694235455501436
3	0.270492682984823
4	0.2780493453308377
5	0.301712772892819
6	0.15877133881745076
7	0.17403123670153642
8	0.14409773370159035
9	0.19153859019715233
10-11	0.2110572536059855
12-13	0.22679758584181137
14-15	0.15818504604922545
16-17	0.14855193014907958
18-19	0.1639339723598789
20-21	0.19385118944959648
22-23	0.1598054941169592
24-25	0.18253248184080295
26-27	0.18182404474586408
28-29	0.20942866258313753
30-31	0.24235063011000968
32-33	0.2117656907009244
34-35	0.1420945667434873
36-37	0.13677721705388862
38-39	0.10733229136079693
40-41	0.09817960981239121
42-43	0.11326850563907784
44-45	0.12684281181451587
46-47	0.19094415447381283
48-49	0.19411176401325217
50-51	0.19962454462559262
52-53	0.1308817175511789
54-55	0.07897037869789914
56-57	0.11544267465457991
58-59	0.1334548913672787
60-61	0.11145262664860234
62-63	0.13128886530689088
64-65	0.10788601230856525
66-67	0.1121366348781985
68-69	0.09818775276750544
70-71	0.12142774666354629
72-73	0.14514817491132728
74-75	0.18153089836175143
76-77	0.16194709131200433
78-79	0.18574894911092774
80-81	0.09635558786680144
82-83	0.13448090371167293
84-85	0.163429109142796
86-87	0.177003415318234
88-89	0.15920291543850545
90-91	0.16141779922957872
92-93	0.11483195302101191
94-95	0.34143410794008155
96-97	0.3411165326906262
98-99	0.38689622634288323
100	0.41906904199924533
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6140277.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.447557587241526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.99992267738382	29.421445388431565
2	9.804344683625077	6.950801455559303
3	2.7538860879465177	2.928556070735624
4	1.2052235082259355	1.7088891885334443
5	0.6563965514114849	1.1633827278112674
6	0.4409765698891531	0.9378925413461995
7	0.28914874526225653	0.7174731759273727
8	0.22791991567424355	0.6463363468913584
9	0.16954017518605463	0.5408806610932819
>10	1.161052261430387	8.104221570499256
>50	0.13963531536642515	3.4896718460837612
>100	0.1281575921022944	8.977804069781584
>500	0.014027554007775537	3.395477206616361
>1k	0.0067128557387500926	4.599805095230539
>5k	0.001111093363655187	2.8367111288552738
>10k+	0.0019444133863965771	23.580651526603855
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	175075	2.851255733251122	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	144098	2.3467670921034993	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	79008	1.2867171953317416	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	75994	1.237631461903103	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	70907	1.1547850365708257	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	61471	1.0011111876548893	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	60803	0.9902321996222646	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	57667	0.9391595851457516	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	54317	0.8846017858803438	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	50044	0.8150120914740492	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	49047	0.7987750389762547	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	41805	0.6808324771016031	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	30789	0.5014268900246682	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	29131	0.47442485086584857	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	25963	0.4228310872620242	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	25380	0.41333640159882035	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	24041	0.3915295678028858	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	22037	0.358892603705012	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	20754	0.33799778088187227	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	20456	0.33314457963378524	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	19482	0.31728210307124577	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	18228	0.296859571644732	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18225	0.29681071391404656	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	18015	0.2933906727660658	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	17839	0.2905243525658533	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	16876	0.27484102101582714	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	15489	0.2522524635289255	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	15042	0.24497266165679496	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	14953	0.24352321564646023	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	14946	0.2434092142748609	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	14484	0.23588512374930318	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	14369	0.23401224407302795	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	14317	0.233165376741147	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	12976	0.21132597112475546	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	12899	0.2100719560371625	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	12380	0.20161956862858144	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	12232	0.1992092539147664	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11548	0.1880696913184861	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	11357	0.18495908246484646	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	11072	0.1803175980497297	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	10986	0.1789170097700804	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	10410	0.16953632547847597	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	9896	0.16116536762103728	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	9688	0.15777789829351346	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	9447	0.1538529939284498	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	9246	0.15057952597252533	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	9133	0.1487392181167071	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9036	0.14715948482454455	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	8835	0.1438860168686201	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG	8431	0.1373065091363142	No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA	7928	0.1291146962913888	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	7719	0.1257109410536365	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	7516	0.12240490127725509	No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA	6727	0.10955531810698442	No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA	6324	0.10299209628490702	No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	6194	0.10087492795520461	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT	6162	0.10035377882789327	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	6157	0.10027234927675087	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1400137159935945E-4	0.0	0.0	0.0	0.0
2	2.7686047388415867E-4	1.628591022847992E-5	0.0	0.0	0.0
3	3.094322943411185E-4	1.628591022847992E-5	0.0	0.0	0.0
4	4.0714775571199804E-4	1.628591022847992E-5	0.0	8.14295511423996E-5	0.0
5	0.0013517305489638334	1.628591022847992E-5	0.0	1.1400137159935945E-4	0.0
6	0.002866320200212466	1.628591022847992E-5	0.0	1.3028728182783937E-4	0.0
7	0.003289753866152944	1.628591022847992E-5	0.0	1.3028728182783937E-4	0.0
8	0.0036480438911795023	1.628591022847992E-5	0.0	1.3028728182783937E-4	0.0
9	0.004218050749176299	1.628591022847992E-5	0.0	1.628591022847992E-4	0.0
10-11	0.008053382607983321	1.628591022847992E-5	0.0	2.5243160854143877E-4	3.257182045695984E-5
12-13	0.015015609230658486	1.628591022847992E-5	0.0	3.501470699123183E-4	3.257182045695984E-5
14-15	0.023541283235267725	1.628591022847992E-5	0.0	4.478625312831978E-4	3.257182045695984E-5
16-17	0.02709975462019059	2.442886534271988E-5	0.0	7.817236909670362E-4	3.257182045695984E-5
18-19	0.029054063847608176	4.885773068543976E-5	0.0	0.0010260123443942351	3.257182045695984E-5
20-21	0.034395842402549594	8.14295511423996E-5	0.0	0.001824021945589751	3.257182045695984E-5
22-23	0.03658629732828014	9.771546137087953E-5	0.0	0.0046659132804594975	3.257182045695984E-5
24-25	0.04977788461334888	1.1400137159935945E-4	0.0	0.010764986661025228	3.257182045695984E-5
26-27	0.09041937358852052	1.1400137159935945E-4	0.0	0.021497401501593495	3.257182045695984E-5
28-29	0.10085864204497615	1.1400137159935945E-4	0.0	0.0680588188448176	3.257182045695984E-5
30-31	0.10415653886624333	1.3028728182783937E-4	0.0	0.173575231215139	3.257182045695984E-5
32-33	0.10683557109882827	1.3028728182783937E-4	0.0	0.3479891868070447	3.257182045695984E-5
34-35	0.10881430919158859	1.3843023694207933E-4	0.0	0.5839231682870334	3.257182045695984E-5
36-37	0.11237278057651146	1.4657319205631927E-4	0.0	0.9145678607007468	3.257182045695984E-5
38-39	0.11609411106371911	1.4657319205631927E-4	0.0	1.4395360339606829	3.257182045695984E-5
40-41	0.1199864436083258	1.4657319205631927E-4	0.0	2.238978795256305	3.257182045695984E-5
42-43	0.1309061464165216	1.4657319205631927E-4	0.0	2.783962352187043	3.257182045695984E-5
44-45	0.14256685814011322	1.4657319205631927E-4	0.0	3.338383594095185	3.257182045695984E-5
46-47	0.14650804841540538	1.4657319205631927E-4	0.0	3.9687704642640718	3.257182045695984E-5
48-49	0.14974894455087287	1.4657319205631927E-4	0.0	4.499039701303378	3.257182045695984E-5
50-51	0.15465914648475956	1.7914501251327912E-4	0.0	5.080153550075998	3.257182045695984E-5
52-53	0.15619002204623667	1.7914501251327912E-4	0.0	5.831096870711207	4.07147755711998E-5
54-55	0.16217509405520306	1.7914501251327912E-4	0.0	6.4536176462397385	4.885773068543976E-5
56-57	0.17508982086638764	1.7914501251327912E-4	0.0	7.082709786545461	4.885773068543976E-5
58-59	0.17994302211447463	1.7914501251327912E-4	0.0	7.732517604661809	4.885773068543976E-5
60-61	0.18329791962154152	1.7914501251327912E-4	0.0	8.26965135286242	4.885773068543976E-5
62-63	0.18622938346266787	1.7914501251327912E-4	0.0	8.81171973186226	6.514364091391968E-5
64-65	0.18811854904917158	1.9543092274175905E-4	0.0	9.413264255016507	6.514364091391968E-5
66-67	0.19065915104481443	1.9543092274175905E-4	0.0	10.021217283845663	8.14295511423996E-5
68-69	0.19372090216776866	1.9543092274175905E-4	0.0	10.789855245944116	8.14295511423996E-5
70-71	0.19744223265497632	1.9543092274175905E-4	0.0	11.756464732779971	8.14295511423996E-5
72-73	0.20802807430348824	1.9543092274175905E-4	0.0	12.465724266185386	8.14295511423996E-5
74-75	0.21908620734862613	1.9543092274175905E-4	0.0	13.147126750144984	8.14295511423996E-5
76-77	0.22266910759889172	1.9543092274175905E-4	0.0	13.860083185172265	8.14295511423996E-5
78-79	0.22505499344736402	1.9543092274175905E-4	0.0	14.495510544556865	8.14295511423996E-5
80-81	0.22855646414648723	1.9543092274175905E-4	0.0	15.21671579311487	8.14295511423996E-5
82-83	0.2300221960670504	1.9543092274175905E-4	0.0	16.110576118960104	8.957250625663956E-5
84-85	0.23566526396121867	1.9543092274175905E-4	0.0	16.89251152676011	9.771546137087953E-5
86-87	0.24655239494895753	2.1171683297023896E-4	0.0	17.637258058553385	1.0585841648511949E-4
88	0.2493372855980276	2.1171683297023896E-4	0.0	18.239551733578143	1.1400137159935945E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	6840	0.0	35.31063	1
ATCTACA	8370	0.0	29.226093	2
TCTACAC	9605	0.0	28.15808	3
TACACTC	9790	0.0	27.243622	5
CTACACT	10635	0.0	25.96467	4
CACTCTT	11385	0.0	23.285812	7
ACACTCT	12050	0.0	22.27362	6
ACTCTTT	13165	0.0	21.529888	8
AGTACTC	2795	0.0	20.70083	5
TATCACG	305	5.156835E-9	18.508629	2
GTACTAG	1155	0.0	17.935556	1
AGTACTT	23550	0.0	17.860668	12-13
GTATAAT	2080	0.0	17.202616	1
TGGTCGC	12615	0.0	17.171877	94
ACTACGC	165	0.001564258	17.104086	3
AAAGTAC	3445	0.0	17.066847	3
GTGATCG	935	0.0	16.59005	8
GAGTACT	23295	0.0	16.563269	12-13
GTATAAG	1735	0.0	16.552917	1
AAGTACT	3530	0.0	16.524532	4
>>END_MODULE