##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765442_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6140277 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3505094314149 33.0 31.0 34.0 31.0 34.0 2 32.485509367085555 34.0 31.0 34.0 31.0 34.0 3 32.552978635980104 34.0 31.0 34.0 31.0 34.0 4 36.09787050323625 37.0 35.0 37.0 35.0 37.0 5 36.01028324943647 37.0 35.0 37.0 35.0 37.0 6 36.11622651551388 37.0 36.0 37.0 35.0 37.0 7 36.038166844915956 37.0 35.0 37.0 35.0 37.0 8 36.07407173976028 37.0 36.0 37.0 35.0 37.0 9 37.79255952133756 39.0 38.0 39.0 35.0 39.0 10-11 37.716144727672706 39.0 37.5 39.0 35.0 39.0 12-13 37.708538311870946 39.0 37.5 39.0 35.0 39.0 14-15 38.98643440678653 40.0 38.0 41.0 35.5 41.0 16-17 38.92047158458813 40.0 38.0 41.0 35.5 41.0 18-19 38.930933816178 40.0 38.0 41.0 35.5 41.0 20-21 38.94565872191108 40.0 38.5 41.0 35.0 41.0 22-23 38.925414358342465 40.0 38.0 41.0 35.0 41.0 24-25 38.89131516379473 40.0 38.0 41.0 35.0 41.0 26-27 38.7176979149312 40.0 38.0 41.0 35.0 41.0 28-29 38.58315699764033 40.0 38.0 41.0 34.5 41.0 30-31 38.37779614828452 40.0 38.0 41.0 34.0 41.0 32-33 38.03038250554495 40.0 37.0 41.0 33.5 41.0 34-35 38.03354913792977 40.0 37.5 41.0 34.0 41.0 36-37 37.994534122809114 40.0 38.0 41.0 33.5 41.0 38-39 37.82955907363788 40.0 37.0 41.0 33.0 41.0 40-41 37.595677198276235 40.0 37.0 41.0 33.0 41.0 42-43 37.36559425576403 40.0 37.0 41.0 33.0 41.0 44-45 37.0062295235215 40.0 36.0 41.0 31.5 41.0 46-47 36.688434251418954 39.0 36.0 41.0 31.0 41.0 48-49 36.45504681629184 39.0 35.5 40.5 30.5 41.0 50-51 36.26721872645159 39.0 35.5 40.5 30.0 41.0 52-53 36.19349949521821 39.0 35.0 40.5 30.0 41.0 54-55 36.21401534491034 39.0 35.0 41.0 30.5 41.0 56-57 36.060198505702594 39.0 35.0 41.0 30.0 41.0 58-59 35.997681944967624 39.0 35.0 41.0 30.0 41.0 60-61 35.64656749198774 39.0 35.0 40.0 29.0 41.0 62-63 35.24580821028107 38.0 35.0 40.0 28.5 41.0 64-65 35.11054208140773 37.5 35.0 40.0 29.0 41.0 66-67 34.70836844982726 37.0 35.0 39.5 29.0 41.0 68-69 34.36643257299304 36.5 34.5 39.0 28.5 41.0 70-71 33.93653747216941 35.5 34.0 39.0 28.0 40.5 72-73 33.47892212354589 35.0 34.0 37.5 27.5 39.5 74-75 33.091384639487764 35.0 34.0 37.0 26.0 39.0 76-77 31.57987351384962 34.0 31.5 35.5 25.0 37.0 78-79 32.37189055151747 35.0 33.0 36.0 26.0 37.0 80-81 32.33550400087813 35.0 34.0 35.5 26.0 37.0 82-83 32.1804968244918 35.0 34.0 35.0 26.0 36.5 84-85 31.975448257464606 35.0 33.0 35.0 26.0 36.0 86-87 31.77261937857201 35.0 33.0 35.0 26.0 36.0 88-89 31.60494958126482 35.0 33.0 35.0 25.0 36.0 90-91 31.45099602184071 35.0 33.0 35.0 25.0 35.0 92-93 31.25817125188326 35.0 33.0 35.0 24.0 35.0 94-95 31.19257925986075 35.0 33.0 35.0 24.0 35.0 96-97 31.093228774532484 35.0 33.0 35.0 23.5 35.0 98-99 30.939077097010447 35.0 33.0 35.0 21.5 35.0 100 30.547819259619722 35.0 32.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38868182370720206 1101 2 0.3551227983893952 1101 3 0.3883536251290991 1101 4 0.2930174200861373 1101 5 0.2815343721741854 1101 6 0.27744333646785435 1101 7 0.2889206567730582 1101 8 0.32519007784566156 1101 9 0.3807449111834629 1101 10-11 0.3608985848983082 1101 12-13 0.3457999525685338 1101 14-15 0.5315391504126836 1101 16-17 0.5165627668121502 1101 18-19 0.4710264280514167 1101 20-21 0.5436410791471005 1101 22-23 0.47397042396237765 1101 24-25 0.4664891882207911 1101 26-27 0.5539927499944355 1101 28-29 0.5109196332288306 1101 30-31 0.5869878082819966 1101 32-33 0.6211362320306151 1101 34-35 0.5677692712922564 1101 36-37 0.5901456274801475 1101 38-39 0.5829849294400447 1101 40-41 0.6532668486580135 1101 42-43 0.6245365859963172 1101 44-45 0.5843105159858908 1101 46-47 0.545328510292812 1101 48-49 0.5568678796477329 1101 50-51 0.6387351702696193 1101 52-53 0.7065625097204418 1101 54-55 0.8203305960641316 1101 56-57 0.7390586552316947 1101 58-59 0.7536088220631143 1101 60-61 0.6885437095420812 1101 62-63 0.7309675661945363 1101 64-65 0.7136605427101586 1101 66-67 0.7190857557455459 1101 68-69 0.6727397510287645 1101 70-71 0.6543169265360902 1101 72-73 0.7094621454967793 1101 74-75 0.7110351594226216 1101 76-77 0.7234740336101844 1101 78-79 0.7873163915705916 1101 80-81 0.7254804067476144 1101 82-83 0.7127142549506189 1101 84-85 0.7539797386029576 1101 86-87 0.7586514428621491 1101 88-89 0.768097471741811 1101 90-91 0.7761554268566258 1101 92-93 0.8001013986152827 1101 94-95 0.81466457846183 1101 96-97 0.9094234623567807 1101 98-99 0.9250599804728807 1101 100 0.9008631733706487 1102 1 0.4214284606163545 1102 2 0.3740395695762473 1102 3 0.3780840552604303 1102 4 0.2584833342074333 1102 5 0.2689988660505378 1102 6 0.2976970438605022 1102 7 0.23471003014377345 1102 8 0.28004780742119095 1102 9 0.3163215859658166 1102 10-11 0.33212256244492266 1102 12-13 0.35084766296152736 1102 14-15 0.46100077920615945 1102 16-17 0.5107432209216398 1102 18-19 0.5249005918898888 1102 20-21 0.48392500517139325 1102 22-23 0.4578718552741634 1102 24-25 0.48143342362484987 1102 26-27 0.4704921843557557 1102 28-29 0.5387124742105769 1102 30-31 0.5857124313944837 1102 32-33 0.6380307680839366 1102 34-35 0.5019647424938753 1102 36-37 0.6299260121144528 1102 38-39 0.5847998918127715 1102 40-41 0.559727041835913 1102 42-43 0.528226148733026 1102 44-45 0.5668624071865196 1102 46-47 0.609020732146142 1102 48-49 0.6320077522560652 1102 50-51 0.6226190790788237 1102 52-53 0.6089134454835587 1102 54-55 0.6629969676149656 1102 56-57 0.7446082162468954 1102 58-59 0.713114395448585 1102 60-61 0.6751446053415933 1102 62-63 0.7036060247061684 1102 64-65 0.6913531672736326 1102 66-67 0.7012760531977165 1102 68-69 0.6399277121472551 1102 70-71 0.6108870196205345 1102 72-73 0.6214906518417749 1102 74-75 0.6293296512085647 1102 76-77 0.5493928121995317 1102 78-79 0.6443362288879868 1102 80-81 0.6762171203868874 1102 82-83 0.6798931474054939 1102 84-85 0.6834832523258498 1102 86-87 0.6923633544749528 1102 88-89 0.736804895134668 1102 90-91 0.7050373528563298 1102 92-93 0.7630803470191978 1102 94-95 0.6580089478976738 1102 96-97 0.7476326625928742 1102 98-99 0.7153608910281939 1102 100 0.760383118282359 1103 1 0.3721098520290269 1103 2 0.30915109630052484 1103 3 0.31255942757572797 1103 4 0.2475170509699538 1103 5 0.23275499605525596 1103 6 0.24866004502746364 1103 7 0.2601153976893684 1103 8 0.2806665963776851 1103 9 0.2734831853934523 1103 10-11 0.3512644283512074 1103 12-13 0.3699068259004079 1103 14-15 0.48518839439699235 1103 16-17 0.510016259667438 1103 18-19 0.5002591595410593 1103 20-21 0.5390493186571774 1103 22-23 0.469444192802996 1103 24-25 0.5005368684675418 1103 26-27 0.6276020636774362 1103 28-29 0.5629734584381154 1103 30-31 0.5954539437401607 1103 32-33 0.5163036493829622 1103 34-35 0.5876026160826413 1103 36-37 0.6078174757210988 1103 38-39 0.6092226641725276 1103 40-41 0.5418845459110173 1103 42-43 0.5535166148680801 1103 44-45 0.6657233492738968 1103 46-47 0.651027544613143 1103 48-49 0.574915643580745 1103 50-51 0.5700720881644301 1103 52-53 0.6014374734813046 1103 54-55 0.6837591025931573 1103 56-57 0.7185839150327027 1103 58-59 0.7232605228369096 1103 60-61 0.8026707721842357 1103 62-63 0.8133180591962699 1103 64-65 0.8101418343478102 1103 66-67 0.8032467860429691 1103 68-69 0.7280541274316334 1103 70-71 0.701425640965688 1103 72-73 0.7160036464098596 1103 74-75 0.6870484356500484 1103 76-77 0.6753472608811428 1103 78-79 0.7367870759747674 1103 80-81 0.6820989438520115 1103 82-83 0.5824547976560126 1103 84-85 0.6725345516089973 1103 86-87 0.7053253623380336 1103 88-89 0.7252465946799163 1103 90-91 0.7228475779820585 1103 92-93 0.7883841266678218 1103 94-95 0.8636782761093542 1103 96-97 0.9155487735650603 1103 98-99 0.9056180183887363 1103 100 0.9437044115686462 1104 1 0.3543005083887678 1104 2 0.27076024858859427 1104 3 0.28339303271614114 1104 4 0.19912343082578587 1104 5 0.20030422031784667 1104 6 0.20692107767693102 1104 7 0.21268574136224316 1104 8 0.23381860720648007 1104 9 0.23447275343763607 1104 10-11 0.33449189282602276 1104 12-13 0.3174304595262143 1104 14-15 0.3854589783473301 1104 16-17 0.36842488356254677 1104 18-19 0.4319775510954713 1104 20-21 0.4326300507967318 1104 22-23 0.415410100040674 1104 24-25 0.3941816791267314 1104 26-27 0.4255579385157162 1104 28-29 0.4183991355095529 1104 30-31 0.4571387263488518 1104 32-33 0.5634928923458773 1104 34-35 0.4993999947751391 1104 36-37 0.5010646326190127 1104 38-39 0.4977829836002101 1104 40-41 0.5641461575095619 1104 42-43 0.45451774830360847 1104 44-45 0.48316004540444624 1104 46-47 0.5430510260269727 1104 48-49 0.6025626368508057 1104 50-51 0.5956867119290692 1104 52-53 0.6184125732345009 1104 54-55 0.6350279660046638 1104 56-57 0.6006820890327305 1104 58-59 0.55885631037075 1104 60-61 0.5503450548853479 1104 62-63 0.5653579033964533 1104 64-65 0.5987538650728794 1104 66-67 0.5246699407446869 1104 68-69 0.647584213757554 1104 70-71 0.557413958697289 1104 72-73 0.4890153096517551 1104 74-75 0.5595321136347593 1104 76-77 0.42728884682111 1104 78-79 0.5806813375836484 1104 80-81 0.5380042245200016 1104 82-83 0.6015173758005545 1104 84-85 0.5919449069152236 1104 86-87 0.6074046185651305 1104 88-89 0.6258162343724507 1104 90-91 0.5630058512460252 1104 92-93 0.5752951886724489 1104 94-95 0.6051389130077105 1104 96-97 0.6085223566347473 1104 98-99 0.6235222906524527 1104 100 0.6693941220397406 1105 1 0.26760187019485926 1105 2 0.20942656839435614 1105 3 0.2466195555109394 1105 4 0.18664699474621926 1105 5 0.19128696775786125 1105 6 0.1545175577927509 1105 7 0.18610092632958697 1105 8 0.16279035312332724 1105 9 0.20473414329421757 1105 10-11 0.21781372696618462 1105 12-13 0.22420810283254866 1105 14-15 0.3316399266348924 1105 16-17 0.28495092075206685 1105 18-19 0.26800452330135727 1105 20-21 0.28338560434149684 1105 22-23 0.2316992727020022 1105 24-25 0.23193645223445003 1105 26-27 0.25629754399913196 1105 28-29 0.3364753016408528 1105 30-31 0.2883086090193814 1105 32-33 0.3112473449842881 1105 34-35 0.3696519590635461 1105 36-37 0.3187885652676741 1105 38-39 0.3167342194807148 1105 40-41 0.32607958660666725 1105 42-43 0.3433773865126071 1105 44-45 0.2959776870518809 1105 46-47 0.3006960101166172 1105 48-49 0.27864601165664027 1105 50-51 0.31035393281914736 1105 52-53 0.26454780751642915 1105 54-55 0.26001203679444984 1105 56-57 0.28342472200863966 1105 58-59 0.2665868786194068 1105 60-61 0.2754673987602345 1105 62-63 0.39964105123460314 1105 64-65 0.4455809360587182 1105 66-67 0.3599262784967223 1105 68-69 0.3546240763704702 1105 70-71 0.33353729791218 1105 72-73 0.3591876278890993 1105 74-75 0.3422979335083838 1105 76-77 0.32348308515979696 1105 78-79 0.4217788884440097 1105 80-81 0.4288814055964778 1105 82-83 0.41258874243253274 1105 84-85 0.4569604968049745 1105 86-87 0.48127741486729647 1105 88-89 0.4620406722315664 1105 90-91 0.5120468939400808 1105 92-93 0.4596043510787595 1105 94-95 0.4744831950810351 1105 96-97 0.4078956506069069 1105 98-99 0.5379878043836221 1105 100 0.5963917769583809 1106 1 0.2032363679149256 1106 2 0.17334211256844156 1106 3 0.15989934819820206 1106 4 0.1119223210532212 1106 5 0.1786006829177822 1106 6 0.1400782711205082 1106 7 0.12226619987353615 1106 8 0.15293434845424514 1106 9 0.1882059151942883 1106 10-11 0.2236163954361814 1106 12-13 0.17412719774399932 1106 14-15 0.250786343002531 1106 16-17 0.23944211424191764 1106 18-19 0.25155235122689135 1106 20-21 0.31065363346383634 1106 22-23 0.24315966197255534 1106 24-25 0.2706047160339722 1106 26-27 0.3230522801358333 1106 28-29 0.27612054243833484 1106 30-31 0.2777377388703073 1106 32-33 0.3252130398288102 1106 34-35 0.3196382855793587 1106 36-37 0.2836452645322751 1106 38-39 0.24060733653708866 1106 40-41 0.302822781099529 1106 42-43 0.36038672030375807 1106 44-45 0.2641433258045467 1106 46-47 0.2623773593968153 1106 48-49 0.3094681419068408 1106 50-51 0.31188405249051954 1106 52-53 0.3036827608736772 1106 54-55 0.24173565592544577 1106 56-57 0.13046364819017953 1106 58-59 0.2042102785378006 1106 60-61 0.19651150251795002 1106 62-63 0.32161337924794964 1106 64-65 0.25483137575078985 1106 66-67 0.21299040582700712 1106 68-69 0.2691091015026075 1106 70-71 0.2200891859451346 1106 72-73 0.29079792747382527 1106 74-75 0.3438873777533331 1106 76-77 0.2085274387971623 1106 78-79 0.34775641150763903 1106 80-81 0.3208894506726736 1106 82-83 0.3457606587560491 1106 84-85 0.341798656048212 1106 86-87 0.3862005244535034 1106 88-89 0.3425661796139394 1106 90-91 0.29278763856395074 1106 92-93 0.3665422682183177 1106 94-95 0.3892333896988376 1106 96-97 0.4326003601392898 1106 98-99 0.41018380089229467 1106 100 0.4106505561652476 1107 1 0.140996108473054 1107 2 0.18535345231347833 1107 3 0.16361907208169413 1107 4 0.11914857590722505 1107 5 0.143392933680488 1107 6 0.15902626793427288 1107 7 0.14169986830464865 1107 8 0.1573041310268266 1107 9 0.17637994985576455 1107 10-11 0.16544434565531674 1107 12-13 0.16122319669381113 1107 14-15 0.278452835323435 1107 16-17 0.2849256329427945 1107 18-19 0.27726157568177 1107 20-21 0.25193588554477486 1107 22-23 0.2581102573750087 1107 24-25 0.2286549021010984 1107 26-27 0.25098551736638086 1107 28-29 0.2716797458578739 1107 30-31 0.23742421003651515 1107 32-33 0.30370442356768734 1107 34-35 0.33406525383814056 1107 36-37 0.2528296569235593 1107 38-39 0.27039955780779934 1107 40-41 0.2727148856230457 1107 42-43 0.28009421172604476 1107 44-45 0.19764232191037223 1107 46-47 0.21011826485079155 1107 48-49 0.2425686804308782 1107 50-51 0.22551564191351048 1107 52-53 0.2548023391626799 1107 54-55 0.3546746770185436 1107 56-57 0.34523808715277227 1107 58-59 0.33061297665858547 1107 60-61 0.33177909995233534 1107 62-63 0.29891707188033934 1107 64-65 0.34179577176615794 1107 66-67 0.2629432940614933 1107 68-69 0.27045914953342276 1107 70-71 0.2829376665786896 1107 72-73 0.27683298235844944 1107 74-75 0.28870554307344776 1107 76-77 0.21912804556432874 1107 78-79 0.26989058751676254 1107 80-81 0.29974907297471276 1107 82-83 0.3624942722771607 1107 84-85 0.26394381954434465 1107 86-87 0.266133847828133 1107 88-89 0.3019045079329423 1107 90-91 0.3550403120829131 1107 92-93 0.3821253368437496 1107 94-95 0.3720712367494521 1107 96-97 0.39353047492080506 1107 98-99 0.41142173814730043 1107 100 0.36541870715773683 1108 1 0.15995979317820996 1108 2 0.12513534847671792 1108 3 0.14430819903421366 1108 4 0.09419955426319149 1108 5 0.13162004514476422 1108 6 0.09806091410361262 1108 7 0.14866165124172426 1108 8 0.12349043562821294 1108 9 0.10319963440341695 1108 10-11 0.16352612810570122 1108 12-13 0.1268417594500022 1108 14-15 0.18407423939987666 1108 16-17 0.17510279560540454 1108 18-19 0.21869332797608365 1108 20-21 0.19390032663851287 1108 22-23 0.12198434558131765 1108 24-25 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-0.8945401810181295 2316 80-81 -0.9266269687106217 2316 82-83 -1.0121779793907066 2316 84-85 -0.9872303265588371 2316 86-87 -0.993472051391187 2316 88-89 -0.9201906815159688 2316 90-91 -0.986690541100046 2316 92-93 -0.9971733387809323 2316 94-95 -0.9930030416559816 2316 96-97 -1.0569260338715587 2316 98-99 -1.1430290477493834 2316 100 -1.1820569047587561 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 20.0 4 35.0 5 92.0 6 209.0 7 598.0 8 1388.0 9 2343.0 10 4411.0 11 8016.0 12 13293.0 13 18946.0 14 21173.0 15 26269.0 16 33666.0 17 44202.0 18 43455.0 19 29300.0 20 21476.0 21 19594.0 22 20932.0 23 23293.0 24 26134.0 25 28842.0 26 35445.0 27 44922.0 28 57508.0 29 73430.0 30 92490.0 31 116197.0 32 148380.0 33 197669.0 34 284028.0 35 457081.0 36 874736.0 37 1688274.0 38 1459689.0 39 222562.0 40 167.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.823549148407 18.225816696988602 13.775170326743947 23.175463827860455 2 13.57254658723087 23.58581358395135 35.83531002297733 27.006329805840444 3 20.6726811238387 28.716077069088087 29.494536420200955 21.116705386872262 4 14.04006692206231 19.59050055885101 33.68823588903237 32.68119663005431 5 12.85034480141611 38.04077423524083 32.964784051857585 16.14409691148547 6 30.68403917282559 32.27592175401859 18.828189672876324 18.211849400279498 7 23.990969788496514 33.47845707937932 22.44820551255261 20.08236761957156 8 28.423961980868288 31.334579856902224 22.15303967557164 18.08841848665785 9 29.045431367462026 15.003902948896531 19.62361664361378 36.32704904002766 10-11 24.503560669982804 27.34635587287023 25.298834238259936 22.85124921888703 12-13 27.678140578999937 24.774973506895538 25.7252889405478 21.82159697355673 14-15 27.058420980030707 22.991495334819586 25.21391950232864 24.736164182821067 16-17 23.427281863668366 26.642804550999898 28.760494029829598 21.169419555502138 18-19 25.596247530852434 25.74159927964162 29.11654474219974 19.545608447306204 20-21 23.874664294774533 25.988317568084206 28.76404534639535 21.37297279074591 22-23 22.260058626974434 28.077798083019427 24.467364703004492 25.194778587001643 24-25 22.94523113625311 28.767619133239457 23.626543918640508 24.660605811866922 26-27 24.432716569366892 25.555898672722904 24.5276547007812 25.483730057129005 28-29 23.92341723457495 24.484859796523693 28.383842480125654 23.207880488775707 30-31 26.525268838394677 25.04299720485595 26.73440789428681 21.69732606246256 32-33 20.971305088711063 26.755221244890482 28.66454548482317 23.608928181575294 34-35 20.219420815498125 27.707183424983977 28.481472351750842 23.59192340776706 36-37 24.038956222984726 27.31493220908438 26.032359777905782 22.61375179002511 38-39 25.3842477614811 24.949332493414587 25.917814938789807 23.748604806314503 40-41 23.05176020493957 27.072222892220164 25.840567614659022 24.035449288181244 42-43 26.236512504942887 25.372410560554727 25.75651468079188 22.63456225371051 44-45 26.103588736845346 24.28233161952513 27.107379049325854 22.50670059430367 46-47 23.58523860647098 25.3194953479979 29.677799507243467 21.417466538287652 48-49 24.413048167585565 26.212161668188195 28.81918615762615 20.555604006600092 50-51 22.323540469614148 27.35117122600137 27.016506143113173 23.308782161271306 52-53 21.431704929836854 28.585677364262768 24.09155016919336 25.891067536707013 54-55 22.770759766087508 27.45207956777068 23.668273379115625 26.108887287026185 56-57 24.07996484453113 24.78159176241224 24.498906278827015 26.63953711422962 58-59 23.646059965966522 24.557002004642587 27.657715137470397 24.139222891920493 60-61 24.45627819765041 25.605995473878533 26.110464338753136 23.827261989717922 62-63 20.317467841159555 26.68393565063813 27.817381716133376 25.18121479206894 64-65 20.07631587433377 26.954793198459058 26.96196730431792 26.006923622889254 66-67 23.33624068401213 26.545061723318202 24.81803678994284 25.300660802726828 68-69 24.377939301435426 24.917719510048162 25.024905228216905 25.679435960299514 70-71 22.53136137017923 26.220706329698157 25.303337292438112 25.944595007684505 72-73 24.382184758631524 24.95363082621533 25.53900169288098 25.12518272227216 74-75 24.575266139626116 23.92381320602365 26.332391887036867 25.16852876731337 76-77 22.452442534924792 25.573718376487392 27.840107605098474 24.13373148348934 78-79 23.21020204965734 25.471818494146554 27.411901061212447 23.906078394983666 80-81 21.515292958071743 26.008557730971688 26.54983673928724 25.926312571669328 82-83 21.228518386962755 27.16568518285989 24.13809305342682 27.467703376750535 84-85 22.018455831630906 26.408309888069407 24.13328934860942 27.439944931690263 86-87 22.349891091275275 24.013744754169362 25.210165053169398 28.42619910138596 88-89 22.533309240841398 23.434000917393018 27.05256508901831 26.980124752747276 90-91 23.543217519914343 24.288636812057128 26.202504773636125 25.965640894392404 92-93 20.604982817855426 25.10834876907509 27.044803863952886 27.241864549116602 94-95 19.88200477553962 25.573696243726946 27.296387108762783 27.247911871970654 96-97 22.0638777943288 24.85993493935895 25.763722337033467 27.31246492927878 98-99 22.93146546971741 23.74390438737536 25.580564199302408 27.744065943604824 100 22.38938910653808 24.971038768880987 26.23380219067915 26.40576993390178 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 782.0 1 474.5 2 245.5 3 349.0 4 301.0 5 326.5 6 568.5 7 1131.5 8 1724.0 9 1799.0 10 1448.5 11 1272.0 12 1417.0 13 1605.5 14 1847.0 15 2153.0 16 2491.5 17 2855.5 18 3343.0 19 3901.5 20 4604.0 21 5601.5 22 6813.0 23 8295.0 24 10106.5 25 12560.0 26 16423.5 27 21043.5 28 25580.0 29 32152.0 30 38950.0 31 44964.0 32 52644.5 33 61503.5 34 70425.0 35 77661.0 36 85636.5 37 93785.5 38 97720.0 39 100206.0 40 102794.0 41 105192.0 42 108207.0 43 114591.0 44 124226.0 45 134471.0 46 154400.0 47 194984.0 48 333763.5 49 508304.5 50 729272.0 51 717883.0 52 446648.5 53 289770.5 54 202367.0 55 156779.5 56 132684.0 57 114808.0 58 104863.0 59 94457.5 60 78822.5 61 63324.5 62 50677.0 63 39376.0 64 28494.0 65 22210.5 66 18042.5 67 13865.0 68 10589.5 69 8631.5 70 7311.0 71 6006.0 72 5229.5 73 5414.0 74 4251.0 75 2999.0 76 2275.5 77 1673.0 78 1221.5 79 741.0 80 534.0 81 463.0 82 428.5 83 367.0 84 240.0 85 117.5 86 78.0 87 45.0 88 21.0 89 12.0 90 9.5 91 6.0 92 3.0 93 3.5 94 4.0 95 2.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009885547508687312 2 0.040551916468915004 3 0.0011725855364505542 4 0.0 5 0.001237729177364474 6 0.0 7 0.0 8 0.0 9 7.817236909670362E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.130061721971174E-4 22-23 0.0016285910228479921 24-25 0.0035666143400371027 26-27 0.007963810101726681 28-29 0.003273467955924464 30-31 0.005586067208368612 32-33 0.0023858858484723084 34-35 0.00627821839307901 36-37 0.0 38-39 8.14295511423996E-6 40-41 0.00204388173367423 42-43 0.0023614569831295886 44-45 0.002442886534271988 46-47 0.00203573877855999 48-49 9.527257483660752E-4 50-51 0.0017670212597900715 52-53 0.0066527943283340475 54-55 0.003306039776381424 56-57 0.029795072763004016 58-59 0.01674191571487736 60-61 0.02029224414468598 62-63 0.015414614031256246 64-65 0.002263741521758709 66-67 0.0015227326063628726 68-69 0.0 70-71 0.0 72-73 0.0024836013098431878 74-75 0.0012865869080499136 76-77 7.735807358527963E-4 78-79 0.002890749065555186 80-81 0.0015960192023910323 82-83 0.0013680164591923134 84-85 0.004340195075889898 86-87 7.084370949388766E-4 88-89 8.387243767667159E-4 90-91 6.758652744819167E-4 92-93 5.944357233395171E-4 94-95 4.6414844151167775E-4 96-97 8.957250625663956E-5 98-99 0.0 100 0.002801176559298546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6140277.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.91962588304133 #Duplication Level Percentage of deduplicated Percentage of total 1 82.66054141065744 28.864751813498035 2 10.122802408746253 7.069689460027375 3 2.7493980550861608 2.8802385446151053 4 1.190989330719331 1.663556074376513 5 0.690151750332676 1.204992046207159 6 0.4178675393566229 0.8755066885800191 7 0.302284012929849 0.7388951249354327 8 0.22115902970753165 0.6178232462434742 9 0.18760576747275093 0.5896010892284375 >10 1.1521884946810907 7.9866407786855635 >50 0.1501109022069271 3.6644752382142056 >100 0.1321407765079337 9.211290618957191 >500 0.013524367995708763 3.218746953231829 >1k 0.0060788970122461535 4.070421625098903 >5k 0.001130957583673702 2.8674271847577617 >10k+ 0.0020262990040820494 24.47594351334318 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 175540 2.858828681507365 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 136727 2.226723647809374 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 82583 1.3449393243985572 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 76845 1.2514907715075396 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 74094 1.2066882324689912 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 69969 1.1395088527765116 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 62858 1.0236997451417909 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 55292 0.9004805483531118 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 54875 0.8936893237878355 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 53211 0.8665895691676451 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 50255 0.8184484185322585 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 47688 0.7766424869757504 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 31252 0.5089672664604545 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 27721 0.45146171744369185 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 26963 0.4391169974905041 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 26881 0.43778155285176873 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 25156 0.4096883577076409 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 24677 0.40188740670819895 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 22797 0.37126989547865674 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 21252 0.34610816417565526 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 21138 0.3442515704096085 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 21005 0.3420855443492207 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 19125 0.31146803311967847 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 18057 0.2940746809956619 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 18041 0.29381410643200623 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 17103 0.27853792263769206 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 15011 0.2444677984397121 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 15001 0.24430493933742728 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 14954 0.24353950155668871 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 14931 0.2431649256214337 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 14758 0.24034746315190667 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 14483 0.2358688378390747 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 14262 0.23226965167858063 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 14189 0.23108078023190157 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 14036 0.22858903596694413 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 13966 0.22744902225095057 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13461 0.21922463758556823 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 13450 0.21904549257305492 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 11807 0.19228774206766241 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 10920 0.17784213969500073 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 10561 0.17199549792297644 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 10428 0.16982947186258862 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT 10101 0.16450397921787566 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9678 0.15761503919122866 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 9538 0.15533501175924147 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 9410 0.15325041524999605 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9399 0.15307127023748276 No Hit GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA 9160 0.14917893769287607 No Hit ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC 9114 0.148429785822366 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 9061 0.14756663258025654 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8615 0.1403031166183545 No Hit CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA 8115 0.13216016150411455 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 7853 0.1278932530242528 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 7017 0.1142782320732436 No Hit GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA 6804 0.11080933319457736 No Hit GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG 6469 0.10535355326803661 No Hit ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT 6449 0.10502783506346701 No Hit TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA 6372 0.10377381997587404 No Hit TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA 6244 0.10168922346662863 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.004641484415116777 0.0 0.0 0.0 0.0 2 0.007019227308474846 0.0 0.0 0.0 0.0 3 0.007279801872130525 0.0 0.0 0.0 0.0 4 0.00807781147332604 1.628591022847992E-5 0.0 1.1400137159935945E-4 1.628591022847992E-5 5 0.02897263429646578 3.257182045695984E-5 0.0 2.280027431987189E-4 1.628591022847992E-5 6 0.06936169166309598 3.257182045695984E-5 0.0 6.840082295961567E-4 1.628591022847992E-5 7 0.07893780687744217 3.257182045695984E-5 0.0 7.654377807385563E-4 1.628591022847992E-5 8 0.0943279920433557 3.257182045695984E-5 0.0 8.631532421094358E-4 1.628591022847992E-5 9 0.10463697321798349 3.257182045695984E-5 0.0 0.0011074418955366347 1.628591022847992E-5 10-11 0.20111470541149853 3.257182045695984E-5 0.0 0.0021578831052735895 4.07147755711998E-5 12-13 0.3282262347447843 3.257182045695984E-5 0.0 0.004535625998631658 4.885773068543976E-5 14-15 0.4764443037341801 3.257182045695984E-5 0.0 0.007849808730127321 4.885773068543976E-5 16-17 0.5457001369807909 3.257182045695984E-5 0.0 0.009690116585945553 4.885773068543976E-5 18-19 0.5771808014524427 3.257182045695984E-5 0.0 0.010650985289425867 6.514364091391968E-5 20-21 0.6649211428083782 3.257182045695984E-5 0.0 0.013273016836211135 6.514364091391968E-5 22-23 0.6903434486750353 6.514364091391968E-5 0.0 0.01670120093930616 6.514364091391968E-5 24-25 0.7963972960828967 9.771546137087953E-5 0.0 0.028101338099242104 6.514364091391968E-5 26-27 1.0161105109753192 9.771546137087953E-5 0.0 0.0604614417232317 6.514364091391968E-5 28-29 1.0754645108030143 9.771546137087953E-5 0.0 0.11257635445436745 6.514364091391968E-5 30-31 1.1034355616204286 1.1400137159935945E-4 0.0 0.21893149120145555 6.514364091391968E-5 32-33 1.1300060241581935 1.1400137159935945E-4 0.0 0.39367116499793087 6.514364091391968E-5 34-35 1.1507054160585914 1.221443267135994E-4 0.0 0.6293038571386926 6.514364091391968E-5 36-37 1.1850524007304557 1.3028728182783937E-4 0.0 0.9590528244898398 6.514364091391968E-5 38-39 1.2202381097790864 1.3028728182783937E-4 0.0 1.4812605359660485 6.514364091391968E-5 40-41 1.25443037830378 1.3028728182783937E-4 0.0 2.27701453859492 6.514364091391968E-5 42-43 1.3241259311265599 1.3028728182783937E-4 0.0 2.8239524047530757 6.514364091391968E-5 44-45 1.4028031634403464 1.3028728182783937E-4 0.0 3.3805803874971767 6.514364091391968E-5 46-47 1.429227052786055 1.3028728182783937E-4 0.0 4.009477096880158 6.514364091391968E-5 48-49 1.4469054083390702 1.3028728182783937E-4 0.0 4.536505437783996 6.514364091391968E-5 50-51 1.4817083984973318 1.628591022847992E-4 0.0 5.113987528575665 6.514364091391968E-5 52-53 1.492139523998673 1.7100205739903915E-4 0.0 5.860191649334387 6.514364091391968E-5 54-55 1.5284896756286401 1.7914501251327912E-4 0.0 6.480684829039472 6.514364091391968E-5 56-57 1.5976559363689944 1.9543092274175905E-4 0.0 7.112113997462981 6.514364091391968E-5 58-59 1.6216125103150882 2.1171683297023896E-4 0.0 7.7608306595940215 6.514364091391968E-5 60-61 1.641546464434748 2.1171683297023896E-4 0.0 8.298167981672488 6.514364091391968E-5 62-63 1.6600716873196437 2.1171683297023896E-4 0.0 8.839869927692188 6.514364091391968E-5 64-65 1.6731574161882272 2.442886534271988E-4 0.0 9.440934016494696 6.514364091391968E-5 66-67 1.692423647988519 2.442886534271988E-4 0.0 10.048545041209053 6.514364091391968E-5 68-69 1.7139780501759123 2.442886534271988E-4 0.0 10.815611412970457 6.514364091391968E-5 70-71 1.741151091392131 2.442886534271988E-4 0.0 11.780576022873234 6.514364091391968E-5 72-73 1.8048697151610589 2.442886534271988E-4 0.0 12.49262044692772 6.514364091391968E-5 74-75 1.8695817794539238 2.6057456365567873E-4 0.0 13.17765468886827 6.514364091391968E-5 76-77 1.8905743177384342 2.6057456365567873E-4 0.0 13.891360275766061 6.514364091391968E-5 78-79 1.904914061694611 2.6057456365567873E-4 0.0 14.526844635836461 6.514364091391968E-5 80-81 1.9320789599557155 2.6057456365567873E-4 0.0 15.248261601227437 6.514364091391968E-5 82-83 1.9419889363297456 2.6057456365567873E-4 0.0 16.14109591472828 6.514364091391968E-5 84-85 1.976791926488007 2.6057456365567873E-4 0.0 16.924765771967614 6.514364091391968E-5 86-87 2.034077615716685 2.7686047388415867E-4 0.0 17.67452836411126 6.514364091391968E-5 88 2.048262643525691 3.094322943411185E-4 0.0 18.279142781343577 6.514364091391968E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATC 2810 0.0 35.120464 3 CGATCTC 2710 0.0 32.254547 5 CCGATCT 3150 0.0 31.180496 4 GATCTAC 2400 0.0 28.99507 1 AGTACTC 2645 0.0 26.11794 5 CGCGCCA 1890 0.0 25.87291 1 TTCCGAT 4225 0.0 23.59106 2 TCTACAC 3570 0.0 22.378345 3 TCTCGCG 1260 0.0 22.378164 8 TACACTC 3490 0.0 21.410114 5 ATCTACA 3550 0.0 20.660202 2 AAGTACT 3465 0.0 20.615204 4 CTCGCGC 1380 0.0 20.432238 9 CGATACA 600 0.0 20.374912 1 AAAGTAC 3560 0.0 20.197086 3 GCGCCAT 2405 0.0 19.939919 2 ATCTCGC 2935 0.0 19.854425 7 CTACACT 4250 0.0 19.79299 4 GATGTAA 1645 0.0 18.86478 1 GTATAAT 1855 0.0 18.503447 1 >>END_MODULE