FastQCFastQC Report
Fri 27 May 2016
ERR765441_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765441_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13308190
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT618400.464676263263449No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA608330.45710949422874186No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG251670.18910911250891368No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189650.1425062311253446No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC181930.1367052920043973No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC181790.13660009362655628No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG162380.12201508995588431No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG157340.11822794835360782No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT153810.11557544639804512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT55450.018.817284
GTATAAG51000.017.975091
GTACTAG48400.017.8722441
CATGGGG300200.017.222154
CATGGGT99550.016.9495644
GTACATA29950.016.481591
CTAGTAC67050.016.4733143
TAGGCAT67100.016.0396735
GTACATG1416400.015.6196991
GTCCTAC44250.015.6174711
CGTTAAC15700.015.5707781
TACATGG1409050.015.2083782
TACATGA50650.014.9412222
ATGGGTA74700.014.8482055
TTTAGGG70050.014.7623072
ACGTTAA14700.014.7030958
GTATTAG58250.014.6886571
CTAGGCA75200.014.6252554
ACATGGG1340100.014.418573
GTACAAG60300.014.3452181
Produced by FastQC (version 0.11.5)