##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765441_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13308190
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.710419373333263	33.0	33.0	33.0	27.0	33.0
2	31.621713922028466	33.0	33.0	33.0	27.0	33.0
3	31.576606961577795	33.0	33.0	33.0	27.0	33.0
4	35.17911293722136	37.0	37.0	37.0	33.0	37.0
5	35.39026817320762	37.0	37.0	37.0	33.0	37.0
6	35.40384890807841	37.0	37.0	37.0	33.0	37.0
7	35.25777524967708	37.0	37.0	37.0	33.0	37.0
8	35.2410855270326	37.0	37.0	37.0	33.0	37.0
9	34.93929204497381	37.0	37.0	37.0	33.0	37.0
10-11	34.953054397329765	37.0	37.0	37.0	33.0	37.0
12-13	34.92703553225495	37.0	37.0	37.0	33.0	37.0
14-15	36.226107569849844	37.0	37.0	40.0	30.0	40.0
16-17	36.4703821857067	37.0	37.0	40.0	33.0	40.0
18-19	36.2907419416164	37.0	37.0	40.0	33.0	40.0
20-21	36.28689930786982	37.0	37.0	40.0	33.0	40.0
22-23	36.47501940534363	37.0	37.0	40.0	33.0	40.0
24-25	36.49369594963703	37.0	37.0	40.0	33.0	40.0
26-27	36.161417630797274	37.0	37.0	40.0	30.0	40.0
28-29	36.039895958804316	37.0	37.0	40.0	27.0	40.0
30-31	35.84195145996563	37.0	37.0	40.0	27.0	40.0
32-33	35.79781236216195	37.0	37.0	40.0	27.0	40.0
34-35	35.75223914747235	37.0	37.0	40.0	27.0	40.0
36-37	35.52259274176278	37.0	37.0	40.0	27.0	40.0
38-39	35.385617615919216	37.0	35.0	40.0	27.0	40.0
40-41	35.53046233184227	37.0	33.0	40.0	27.0	40.0
42-43	35.44939135224249	37.0	33.0	40.0	27.0	40.0
44-45	35.39755530241152	37.0	33.0	40.0	27.0	40.0
46-47	35.35410348815279	37.0	33.0	40.0	27.0	40.0
48-49	35.18736364599543	37.0	33.0	40.0	27.0	40.0
50-51	35.04027737806568	37.0	33.0	40.0	27.0	40.0
52-53	34.93007456310738	37.0	33.0	40.0	27.0	40.0
54-55	34.80428871243949	37.0	33.0	40.0	27.0	40.0
56-57	34.595580728859446	37.0	33.0	40.0	27.0	40.0
58-59	34.40906716089866	37.0	33.0	38.5	27.0	40.0
60-61	34.13452772315394	37.0	33.0	37.0	27.0	40.0
62-63	33.89882313071875	37.0	33.0	37.0	27.0	40.0
64-65	33.70641544793094	37.0	33.0	37.0	27.0	40.0
66-67	33.48954275525072	37.0	33.0	37.0	27.0	40.0
68-69	33.28865037243983	37.0	33.0	37.0	27.0	40.0
70-71	33.051592478015415	37.0	33.0	37.0	22.0	37.0
72-73	32.80880649434671	37.0	33.0	37.0	22.0	37.0
74-75	32.58811250816227	35.0	33.0	37.0	22.0	37.0
76-77	32.37894236556586	33.0	33.0	37.0	22.0	37.0
78-79	32.186157584164334	33.0	33.0	37.0	22.0	37.0
80-81	32.04849494935074	33.0	33.0	37.0	22.0	37.0
82-83	31.914376072178108	33.0	33.0	37.0	22.0	37.0
84-85	31.750266264608484	33.0	33.0	37.0	22.0	37.0
86-87	31.5410271419329	33.0	33.0	37.0	22.0	37.0
88-89	31.39282475678511	33.0	33.0	37.0	22.0	37.0
90-91	31.304737045383334	33.0	33.0	37.0	22.0	37.0
92-93	31.200419290677395	33.0	30.0	37.0	22.0	37.0
94-95	31.073261991300093	33.0	27.0	37.0	18.5	37.0
96-97	30.97830046760679	33.0	27.0	37.0	15.0	37.0
98-99	30.844980271547072	33.0	27.0	37.0	15.0	37.0
100	30.749235470789042	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	11.0
7	192.0
8	1180.0
9	2437.0
10	4175.0
11	7373.0
12	13373.0
13	22376.0
14	32471.0
15	38824.0
16	44173.0
17	52635.0
18	62434.0
19	74104.0
20	87344.0
21	103397.0
22	119781.0
23	127401.0
24	132974.0
25	145825.0
26	169012.0
27	203146.0
28	251647.0
29	313502.0
30	390085.0
31	483814.0
32	622637.0
33	821784.0
34	1088533.0
35	1482916.0
36	2213757.0
37	3055824.0
38	1138072.0
39	981.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09588823385289	18.717858560789754	12.892842109710598	25.293411095646757
2	15.748986926151867	21.03820386694882	37.34823621992205	25.864572986977265
3	17.89669578730224	26.181469883978682	29.422707331487093	26.499126997231993
4	12.65333498783662	17.593988952056808	36.52905753966091	33.223618520445655
5	14.147307241328672	37.25592950117442	33.36088712435069	15.235876133146212
6	31.67850112361763	36.157443411558525	17.574839383070447	14.589216081753396
7	27.375576785715495	31.785013694302688	22.314048841196083	18.525360678785734
8	27.16567772674749	32.46340536547217	20.36715507606437	20.00376183171597
9	27.142229361316872	14.412684390430993	19.860069770489595	38.58501647776254
10-11	25.152053622334876	25.193562840082624	26.94990470876764	22.704478828814867
12-13	26.445973714200356	23.04334934222381	26.47764258279705	24.033034360778785
14-15	24.422783812619617	23.89970949875827	24.428784025801896	27.248722662820217
16-17	23.754761833282753	26.363205873553003	25.613820773626035	24.268211519538212
18-19	24.288203110986505	25.51124055540781	27.336908865485682	22.863647468120003
20-21	25.248345786114612	24.97950608779191	26.409685638773368	23.362462487320116
22-23	24.48004824422678	24.902095041171847	25.96139823030598	24.656458484295392
24-25	24.139838320965453	25.57285517325145	25.922679090653233	24.364627415129856
26-27	24.497011448169953	25.10434272510874	26.092267467186588	24.30637835953472
28-29	23.948182853833398	25.006303505560997	26.8185895453372	24.22692409526841
30-31	24.629480784465134	25.092859085576496	26.402722844139415	23.87493728581895
32-33	23.844107128426696	25.28440977318557	26.0806413723504	24.790841726037335
34-35	24.13695099433479	25.356813227054243	26.629168722480507	23.877067056130468
36-37	24.645518696579913	25.150731703356584	26.02994171151168	24.17380788855183
38-39	24.740802118309706	24.718799671613578	26.127456230336932	24.41294197973978
40-41	24.21842310716485	25.218254813300376	26.390782262736384	24.172539816798388
42-43	25.017751284430613	24.998744403007237	25.936524985703485	24.046979326858665
44-45	24.628228356247114	25.254224449222495	25.59782307339947	24.51972412113092
46-47	24.129695083311255	25.258365486786083	26.301944154798534	24.30999527510413
48-49	24.97728196466389	25.380960145323243	25.643617515017812	23.998140374995053
50-51	23.963205118838115	25.81087933501528	25.455608252896727	24.77030729324988
52-53	23.887539674906222	25.68224683201885	25.03647761974608	25.393735873328843
54-55	24.22727282973868	25.619622202568493	25.043416873368958	25.10968809432387
56-57	24.56696966304208	24.962166154826466	24.91761839889572	25.553245783235734
58-59	24.094102954646726	24.939090139230053	25.796749971258297	25.17005693486492
60-61	24.313174819415714	25.356205464454597	25.413174143140427	24.917445572989262
62-63	23.682849463969433	25.37522064564724	25.335207429638917	25.606722460744407
64-65	23.54343698432756	25.2439976812739	25.587639983094597	25.62492535130395
66-67	24.538391957050084	25.07832793012884	25.26446196286306	25.11881814995801
68-69	24.57869602682435	24.668521307486085	25.26361191330891	25.48917075238065
70-71	23.762409233024293	25.016580960642603	25.52224719139961	25.698762614933496
72-73	24.613643058093682	25.021618315379435	25.335523468308164	25.029215158218715
74-75	24.849610335479046	24.839586424242807	25.249702654649546	25.061100585628605
76-77	24.201260781294867	25.13204570137765	25.721491595786993	24.94520192154049
78-79	24.407697395941703	25.203895135543984	25.553431800525228	24.834975667989085
80-81	23.835885497224865	25.118280600869358	25.837830315541172	25.2080035863646
82-83	23.94854513215287	25.269688708080956	25.2116078340613	25.570158325704874
84-85	24.124639124002105	25.381312847916288	24.836316404593198	25.65773162348841
86-87	24.08538397532925	24.867218280365048	25.19408813350243	25.853309610803276
88-89	24.070099614961514	24.48530817111848	25.538224859905483	25.90636735401452
90-91	24.422751786677942	24.795159444378505	25.224053075624436	25.55803569331911
92-93	23.942851895641468	24.91484277793867	25.253506644029212	25.88879868239065
94-95	23.235316075065054	25.081888737269864	25.77461179191054	25.90818339575454
96-97	24.195836980280035	24.858697096228894	25.168850376196577	25.776615547294483
98-99	23.914824484100272	24.905796062767433	25.14394394672707	26.035435506405218
100	23.63409976153146	25.169789075383036	25.176394206871365	26.01971695621414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1054.0
1	1032.0
2	1344.5
3	1695.0
4	1493.5
5	1320.0
6	1673.0
7	3135.5
8	5133.5
9	5177.5
10	3555.0
11	2549.0
12	2382.5
13	2514.5
14	2797.5
15	3348.0
16	3934.5
17	4630.0
18	5613.5
19	6742.5
20	8344.0
21	10524.0
22	13269.5
23	16688.5
24	20645.0
25	25925.5
26	33980.5
27	43050.5
28	52238.5
29	65952.0
30	82381.0
31	97273.5
32	114750.0
33	133432.0
34	152535.5
35	169922.0
36	186575.5
37	210094.0
38	225631.0
39	235109.5
40	245349.0
41	256209.0
42	274320.5
43	307122.5
44	350500.5
45	395646.5
46	453724.5
47	510934.0
48	568061.0
49	629484.5
50	728103.0
51	733354.5
52	633404.0
53	594906.0
54	577989.0
55	549885.5
56	516454.0
57	472919.0
58	440775.5
59	406902.5
60	348895.0
61	284692.0
62	231391.0
63	182874.0
64	137956.0
65	108123.5
66	83081.5
67	60510.0
68	46121.0
69	36915.0
70	32137.0
71	29076.5
72	25336.0
73	25466.5
74	21046.0
75	15248.0
76	11135.0
77	7485.5
78	5279.5
79	3196.0
80	2071.0
81	1606.0
82	1197.5
83	794.5
84	548.0
85	363.0
86	244.5
87	147.0
88	83.0
89	56.5
90	41.5
91	28.0
92	21.0
93	18.5
94	16.0
95	10.5
96	5.5
97	3.0
98	1.5
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04982646024741155
2	0.025435464927987952
3	0.022264485253065968
4	0.02321127065363509
5	0.01574969999676891
6	0.025525634966137394
7	0.050142055380934594
8	0.006334445179998182
9	0.004192906773948974
10-11	0.0022730363783504746
12-13	0.0012623805340921644
14-15	0.0024759189641867154
16-17	0.0071121617590371045
18-19	0.005312518080971191
20-21	0.00893810503156327
22-23	0.01920997521075368
24-25	0.02627329486579317
26-27	0.028828112613360643
28-29	0.045595982624233644
30-31	0.04340184502926393
32-33	0.041064938207224275
34-35	0.04871436311023512
36-37	0.04567112432269152
38-39	0.05557855726436127
40-41	0.05298616866756486
42-43	0.058189731285772144
44-45	0.05851659767406387
46-47	0.06331439512059867
48-49	0.06563251651802386
50-51	0.0341594161189463
52-53	2.2542509537360077E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	6.537327765834423E-4
64-65	1.540404818386272E-4
66-67	2.855384541398943E-4
68-69	9.956275045667367E-4
70-71	3.381376430604011E-5
72-73	2.2542509537360077E-4
74-75	8.641295322654697E-5
76-77	1.0144129291812034E-4
78-79	7.514169845786692E-6
80-81	4.5085019074720156E-5
82-83	1.6906882153020056E-4
84-85	9.129716362630831E-4
86-87	0.003358833921066651
88-89	0.0022504938688131144
90-91	0.0034677893838305583
92-93	0.003396404770295585
94-95	0.0026036598515650888
96-97	0.0019085991408298198
98-99	0.003700728649049946
100	0.0026675302952542754
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.330819E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.27966934688808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.35205372177063	27.3454030866278
2	13.458977087801843	10.034924311611908
3	5.143926964471395	5.7529168914010596
4	2.3994643440995596	3.578049494307171
5	1.353925670768664	2.523695066325972
6	0.8716398689100908	1.9496667661519833
7	0.575610842253034	1.5021007310173804
8	0.41075130473801635	1.2250138259548888
9	0.30264447766145924	1.0154237451192771
>10	1.8130549375118326	12.59789728626926
>50	0.15974006507913482	4.128736043362179
>100	0.12438006319840895	9.521617829408518
>500	0.01910279820350599	5.020172952221288
>1k	0.013650207722534795	9.594093850036439
>5k	8.240820899637007E-4	1.9607962985016671
>10k+	2.53563719988831E-4	2.24949182168324
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	61840	0.464676263263449	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	60833	0.45710949422874186	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	25167	0.18910911250891368	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18965	0.1425062311253446	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	18193	0.1367052920043973	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	18179	0.13660009362655628	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	16238	0.12201508995588431	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	15734	0.11822794835360782	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	15381	0.11557544639804512	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.514169845786693E-6	0.0
2	0.0	0.0	0.0	7.514169845786693E-6	0.0
3	7.514169845786693E-6	0.0	0.0	7.514169845786693E-6	0.0
4	7.514169845786693E-6	0.0	0.0	2.2542509537360078E-5	0.0
5	7.514169845786693E-6	0.0	0.0	2.2542509537360078E-5	0.0
6	7.514169845786693E-6	0.0	0.0	2.2542509537360078E-5	7.514169845786693E-6
7	7.514169845786693E-6	0.0	0.0	3.005667938314677E-5	2.2542509537360078E-5
8	7.514169845786693E-6	0.0	0.0	3.005667938314677E-5	2.2542509537360078E-5
9	7.514169845786693E-6	0.0	0.0	3.005667938314677E-5	4.5085019074720156E-5
10-11	3.005667938314677E-5	0.0	0.0	3.381376430604011E-5	4.8842103997613504E-5
12-13	3.005667938314677E-5	3.7570849228933463E-6	0.0	4.508501907472015E-5	1.0519837784101369E-4
14-15	3.757084922893346E-5	1.5028339691573385E-5	0.0	6.011335876629354E-5	1.3149797230126712E-4
16-17	4.5085019074720156E-5	1.5028339691573385E-5	0.0	1.0144129291812035E-4	1.540404818386272E-4
18-19	4.5085019074720156E-5	1.5028339691573385E-5	0.0	1.277408873783738E-4	1.5779756676152055E-4
20-21	5.2599188920506845E-5	1.5028339691573385E-5	0.0	3.005667938314677E-4	1.9161133106756066E-4
22-23	6.011335876629354E-5	1.5028339691573385E-5	0.0	0.001149667986405364	2.179109255278141E-4
24-25	9.01700381494403E-5	2.2542509537360078E-5	0.0	0.003497846063213705	2.366963501422808E-4
26-27	1.0895546276390704E-4	2.2542509537360078E-5	0.0	0.0057596111867955	2.479676049109608E-4
28-29	1.1646963260969373E-4	2.2542509537360078E-5	0.0	0.017376517768381727	2.479676049109608E-4
30-31	1.4652631199284048E-4	2.2542509537360078E-5	0.0	0.05004061408801648	2.5548177475674753E-4
32-33	1.5779756676152055E-4	2.2542509537360078E-5	0.0	0.11165680682346735	2.742671993712143E-4
34-35	1.8034007629888062E-4	2.2542509537360078E-5	0.0	0.19353120146315916	3.005667938314677E-4
36-37	2.028825858362407E-4	2.6299594460253422E-5	0.0	0.31713178125650443	3.080809636772544E-4
38-39	2.141538406049207E-4	3.005667938314677E-5	0.0	0.503039857411113	3.193522184459344E-4
40-41	2.3293926521938745E-4	3.005667938314677E-5	0.0	0.7318726288097781	3.3062347321461446E-4
42-43	2.4045343506517414E-4	4.132793415182681E-5	0.0	0.9376218704421864	3.343805581375078E-4
44-45	2.5548177475674753E-4	5.635627384340019E-5	0.0	1.1796570382598985	3.456518129061879E-4
46-47	2.6675302952542757E-4	6.011335876629354E-5	0.0	1.4503813065488245	3.606801525977612E-4
48-49	2.7051011444832095E-4	6.011335876629354E-5	0.0	1.7223303845226137	3.832226621351213E-4
50-51	2.7051011444832095E-4	6.011335876629354E-5	0.0	2.0159578425014972	3.832226621351213E-4
52-53	2.742671993712143E-4	6.011335876629354E-5	0.0	2.3142929278887663	4.2079351136405476E-4
54-55	2.855384541398943E-4	6.011335876629354E-5	0.0	2.612473972794197	4.320647661327348E-4
56-57	2.93052623985681E-4	7.138461353497358E-5	0.0	2.9522872757302085	4.4333602090141483E-4
58-59	2.93052623985681E-4	8.265586830365362E-5	0.0	3.318738310769534	4.5085019074720154E-4
60-61	3.005667938314677E-4	8.265586830365362E-5	0.0	3.686733507712168	4.658785304387749E-4
62-63	3.2310930336882775E-4	9.017003814944031E-5	0.0	4.064880348116461	5.485343987424286E-4
64-65	3.2310930336882775E-4	9.017003814944031E-5	0.0	4.468470167618587	5.485343987424286E-4
66-67	3.2686638829172113E-4	9.017003814944031E-5	0.0	4.9009745126873	5.485343987424286E-4
68-69	3.456518129061878E-4	9.392712307233365E-5	0.0	5.377466808033249	5.485343987424286E-4
70-71	3.456518129061878E-4	9.7684207995227E-5	0.0	5.912524543157259	5.485343987424286E-4
72-73	3.494088978290812E-4	9.7684207995227E-5	0.0	6.41893826282913	5.898623328942554E-4
74-75	3.5316598275197454E-4	9.7684207995227E-5	0.0	6.926772160601855	5.936194178171487E-4
76-77	3.5316598275197454E-4	1.1271254768680039E-4	0.0	7.484620372868136	5.97376502740042E-4
78-79	3.5316598275197454E-4	1.4276922706994716E-4	0.0	8.057372941023536	6.086477575087221E-4
80-81	3.5316598275197454E-4	1.4276922706994716E-4	0.0	8.669905524342528	6.311902670460822E-4
82-83	3.5316598275197454E-4	1.4276922706994716E-4	0.0	9.323070981102614	6.762752861208023E-4
84-85	3.5316598275197454E-4	1.4276922706994716E-4	0.0	9.969725409691325	6.762752861208023E-4
86-87	3.5316598275197454E-4	1.4276922706994716E-4	0.0	10.634909781119745	7.025748805810557E-4
88	3.5316598275197454E-4	1.5028339691573384E-4	0.0	11.159158382920593	7.363886448870958E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACT	5545	0.0	18.81728	4
GTATAAG	5100	0.0	17.97509	1
GTACTAG	4840	0.0	17.872244	1
CATGGGG	30020	0.0	17.22215	4
CATGGGT	9955	0.0	16.949564	4
GTACATA	2995	0.0	16.48159	1
CTAGTAC	6705	0.0	16.473314	3
TAGGCAT	6710	0.0	16.039673	5
GTACATG	141640	0.0	15.619699	1
GTCCTAC	4425	0.0	15.617471	1
CGTTAAC	1570	0.0	15.570778	1
TACATGG	140905	0.0	15.208378	2
TACATGA	5065	0.0	14.941222	2
ATGGGTA	7470	0.0	14.848205	5
TTTAGGG	7005	0.0	14.762307	2
ACGTTAA	1470	0.0	14.703095	8
GTATTAG	5825	0.0	14.688657	1
CTAGGCA	7520	0.0	14.625255	4
ACATGGG	134010	0.0	14.41857	3
GTACAAG	6030	0.0	14.345218	1
>>END_MODULE