FastQCFastQC Report
Fri 27 May 2016
ERR765440_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765440_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12491598
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192410.15403153383578308No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC161200.12904674005679656No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA161030.12891064858155057No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC150360.12036890716463977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA307300.039.819711
CAACGCA348800.031.9845735
ATCAACG351800.031.7118233
TATCAAC392750.030.951842
GTACAAG41550.029.0434591
GGTATCA178100.028.6583981
AACGCAG412000.028.183676
CGCAGAG407500.026.916338
ACGCAGA410800.026.9058467
GCAGAGT410800.025.328839
TCAACGC454950.024.8314764
GTACATG544750.023.6953851
TACATGG544150.023.246072
GTATAAG67250.023.041351
AGAGTAC376650.021.85518610-11
CTATTGA83550.021.6366549
GTGATCG57000.021.2527668
CGTTAAC24200.021.1493661
ACGTTAA18900.021.1167818
ACATGGG580400.020.7910463
Produced by FastQC (version 0.11.5)