##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765440_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12491598
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19440875378795	33.0	33.0	33.0	33.0	33.0
2	32.16147053403416	33.0	33.0	33.0	33.0	33.0
3	32.11993181336767	33.0	33.0	33.0	33.0	33.0
4	35.937093636858954	37.0	37.0	37.0	33.0	37.0
5	35.983635480424525	37.0	37.0	37.0	33.0	37.0
6	35.92444441455769	37.0	37.0	37.0	33.0	37.0
7	35.88883375849911	37.0	37.0	37.0	33.0	37.0
8	35.8687430543314	37.0	37.0	37.0	33.0	37.0
9	35.84385472539222	37.0	37.0	37.0	33.0	37.0
10-11	35.82272632372575	37.0	37.0	37.0	33.0	37.0
12-13	35.80680242031484	37.0	37.0	37.0	33.0	37.0
14-15	37.67207258030558	40.0	37.0	40.0	33.0	40.0
16-17	37.738001975407784	40.0	37.0	40.0	33.0	40.0
18-19	37.796025536524624	40.0	37.0	40.0	33.0	40.0
20-21	37.82090349849555	40.0	37.0	40.0	33.0	40.0
22-23	37.81737816891001	40.0	37.0	40.0	33.0	40.0
24-25	37.81902475567978	40.0	37.0	40.0	33.0	40.0
26-27	37.799715216579976	40.0	37.0	40.0	33.0	40.0
28-29	37.77766263371588	40.0	37.0	40.0	33.0	40.0
30-31	37.71405804125301	40.0	37.0	40.0	33.0	40.0
32-33	37.64241060271072	40.0	37.0	40.0	33.0	40.0
34-35	37.541349353381364	40.0	37.0	40.0	33.0	40.0
36-37	37.44693104917401	40.0	37.0	40.0	33.0	40.0
38-39	37.33781378491366	40.0	37.0	40.0	33.0	40.0
40-41	37.22660943779971	40.0	37.0	40.0	33.0	40.0
42-43	37.07761444932826	40.0	37.0	40.0	33.0	40.0
44-45	36.89250882873432	40.0	37.0	40.0	33.0	40.0
46-47	36.69014212593136	40.0	37.0	40.0	33.0	40.0
48-49	36.48163849813291	37.0	37.0	40.0	33.0	40.0
50-51	36.260067927257985	37.0	37.0	40.0	33.0	40.0
52-53	36.11194656600381	37.0	37.0	40.0	33.0	40.0
54-55	35.95042896032997	37.0	37.0	40.0	30.0	40.0
56-57	35.77029864393651	37.0	37.0	40.0	27.0	40.0
58-59	35.54159435806372	37.0	37.0	40.0	27.0	40.0
60-61	35.38818784434145	37.0	35.0	40.0	27.0	40.0
62-63	35.18854173020938	37.0	33.0	40.0	27.0	40.0
64-65	34.98499707563436	37.0	33.0	37.0	27.0	40.0
66-67	34.80684608966763	37.0	33.0	37.0	27.0	40.0
68-69	34.5796142735301	37.0	33.0	37.0	27.0	40.0
70-71	34.41985865219166	37.0	33.0	37.0	27.0	40.0
72-73	34.228554945492164	37.0	33.0	37.0	27.0	40.0
74-75	34.009628311766036	37.0	33.0	37.0	27.0	37.0
76-77	33.82758871202868	37.0	33.0	37.0	27.0	37.0
78-79	33.656455242956106	37.0	33.0	37.0	27.0	37.0
80-81	33.51027254479371	37.0	33.0	37.0	27.0	37.0
82-83	33.35435442286888	37.0	33.0	37.0	27.0	37.0
84-85	33.21970996024688	37.0	33.0	37.0	27.0	37.0
86-87	33.117762275090826	37.0	33.0	37.0	27.0	37.0
88-89	33.04571929067842	37.0	33.0	37.0	27.0	37.0
90-91	32.920696135114184	37.0	33.0	37.0	27.0	37.0
92-93	32.83365238778897	37.0	33.0	37.0	27.0	37.0
94-95	32.740887194736814	37.0	33.0	37.0	22.0	37.0
96-97	32.65461052300915	37.0	33.0	37.0	22.0	37.0
98-99	32.568811212144354	37.0	33.0	37.0	22.0	37.0
100	32.48208555862909	37.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	40.0
5	79.0
6	330.0
7	995.0
8	2161.0
9	4230.0
10	4700.0
11	5205.0
12	6948.0
13	11294.0
14	19882.0
15	27535.0
16	31181.0
17	36547.0
18	45252.0
19	53657.0
20	64204.0
21	77241.0
22	93667.0
23	92211.0
24	82438.0
25	79810.0
26	86087.0
27	96379.0
28	115871.0
29	146924.0
30	186840.0
31	241686.0
32	325499.0
33	450027.0
34	623732.0
35	922448.0
36	1765700.0
37	4188524.0
38	2598170.0
39	4097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.14936289439085	20.40467615169522	13.027803337950846	24.41815761596309
2	16.136945348092134	21.253686951960667	38.181867961728386	24.427499738218806
3	17.69755413078808	26.953786603441	30.386698977572923	24.961960288198
4	12.956757013794393	17.69815999522239	37.98526017247753	31.35982281850569
5	13.497174058902253	38.028595566636035	34.05979728028537	14.41443309417634
6	29.777274647476343	38.64201089806704	17.79280319620275	13.787911258253866
7	25.820747673756394	33.02013881650691	22.929532314440472	18.22958119529623
8	26.41042403061642	33.15640641013263	20.341528761972647	20.0916407972783
9	26.707527731840237	14.528461450648669	20.788341091347963	37.97566972616313
10-11	24.898327659919893	25.849414942747917	27.65426409015084	21.597993307181355
12-13	25.726964636550104	24.343050424773516	27.25504775289759	22.67493718577879
14-15	23.56158406924674	24.697779000825555	25.46969204212844	26.270944887799264
16-17	22.725875733171723	27.123921382455755	26.099748581090303	24.050454303282216
18-19	22.721174387537896	26.94296859463481	28.211288978099923	22.124568039727375
20-21	24.508541298472736	25.91615257135731	27.092923890674104	22.482382239495852
22-23	24.55365881284048	25.43340724292069	26.80569051908056	23.207243425158264
24-25	23.607195663914275	26.04853816305102	27.31465257949181	23.029613593542894
26-27	23.495564173144672	26.31570517979461	27.04925863481949	23.139472012241228
28-29	23.066533308301942	26.345151588350664	27.290063894119783	23.29825120922761
30-31	23.56362177237808	26.001049602214376	27.256247305663905	23.179081319743634
32-33	23.63623722361009	26.3161792246232	26.577037694215065	23.47054585755164
34-35	23.76514690693069	26.148003558001566	27.152557106377657	22.93429242869009
36-37	23.614200415527716	25.84730243600389	27.070384637273108	23.468112511195287
38-39	23.674910670115114	25.732914377882075	27.37047006487631	23.2217048871265
40-41	23.441705078729495	25.506259769032276	27.693966384577983	23.358068767660242
42-43	23.481167139303462	25.77462863674803	27.09947470457714	23.644729519371367
44-45	23.300075409686517	26.25733968994289	26.974234282931008	23.468350617439583
46-47	23.324608665364465	26.009208033955964	26.68777715423761	23.978406146441955
48-49	23.944672438846936	25.761023247954558	26.634870690535408	23.6594336226631
50-51	23.424179456630647	26.159365397716766	26.12343985984075	24.29301528581184
52-53	23.14452661228005	26.33981573942283	26.047282192124417	24.4683754561727
54-55	23.20889725278405	26.342000399219888	26.050543448484454	24.398558899511613
56-57	23.638937277433662	25.960228573954826	25.76479006588526	24.63604408272625
58-59	23.024061078995352	25.97086279350836	26.560791773534735	24.444284353961553
60-61	23.359428773440364	26.079967906028546	26.121591076735445	24.43901224379565
62-63	23.46863926648115	25.813040779964364	25.80322716261937	24.915092790935116
64-65	22.806344238328798	25.91330217894904	26.187836977300798	25.092516605421363
66-67	23.645290551553476	25.577684278687297	25.99914690455001	24.77787826520922
68-69	23.134343238514486	25.48049854148564	26.287540218552536	25.09761800144734
70-71	23.061731086453168	25.633610411142932	26.07380448801259	25.2308540143913
72-73	23.65733023567084	25.552516378303853	26.124216970749224	24.665936415276086
74-75	23.523354371085052	25.924434678830043	26.12436753400208	24.42784341608282
76-77	23.22633474129069	25.900472604178894	25.98531686168592	24.887875792844497
78-79	23.112927792327483	26.152637341168035	25.82565634556442	24.908778520940068
80-81	23.02119984222412	25.979273317237823	26.244574151879586	24.75495268865847
82-83	23.401899443666867	25.3194191331196	26.732795950330374	24.545885472883164
84-85	23.52157350421399	25.74614574167003	25.851371898025604	24.88090885609038
86-87	22.847491183032346	25.817001366977316	26.331245405139292	25.004262044851046
88-89	23.283511049663147	25.107240772008645	26.256220445227974	25.35302773310024
90-91	23.25275744913025	25.294421636910556	26.20369583740064	25.249125076558553
92-93	23.425057924778674	25.08656039847908	26.2537991837187	25.234582493023545
94-95	22.583751081743564	25.17376253480425	26.747698669008546	25.49478771444364
96-97	22.94287160964418	25.30738948566546	26.215150061346588	25.534588843343776
98-99	22.678834471047622	25.55988309050446	26.092946099351934	25.668336339095983
100	22.99837361664347	25.267592700448848	25.84881559822927	25.885218084678414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	639.0
1	503.0
2	511.0
3	712.0
4	658.5
5	599.0
6	821.0
7	1634.5
8	2562.5
9	2510.5
10	1865.5
11	1601.5
12	1791.5
13	2091.0
14	2551.5
15	3174.5
16	3830.5
17	4767.5
18	6007.0
19	7295.5
20	8760.0
21	10631.5
22	13248.0
23	16635.5
24	20591.5
25	25993.0
26	34110.0
27	42941.0
28	53224.5
29	71457.0
30	93459.5
31	113467.0
32	138909.5
33	165858.5
34	192772.5
35	214299.5
36	234290.0
37	269898.0
38	290951.0
39	300075.0
40	302599.0
41	303042.0
42	315461.0
43	343766.0
44	389025.0
45	444345.5
46	500229.5
47	550071.5
48	577691.0
49	568919.5
50	554669.5
51	552880.0
52	546443.5
53	539647.0
54	524704.5
55	481714.0
56	451455.5
57	406308.5
58	350211.5
59	310912.0
60	252142.5
61	192373.5
62	148206.0
63	115184.5
64	87038.0
65	62350.5
66	47936.0
67	37882.0
68	31092.5
69	26199.0
70	21993.5
71	18836.0
72	16096.0
73	14664.5
74	11993.0
75	9100.0
76	7133.5
77	5479.5
78	4128.5
79	2858.0
80	2058.0
81	1533.0
82	1158.0
83	884.5
84	630.0
85	416.0
86	294.5
87	155.0
88	73.0
89	56.0
90	41.5
91	29.0
92	21.5
93	18.5
94	17.0
95	13.5
96	7.5
97	5.0
98	5.5
99	4.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02911557032174747
2	0.0020013452242059024
3	3.7625290215070965E-4
4	0.0
5	0.002889942503753323
6	2.0013452242059022E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.841910218372381E-4
16-17	0.003946652782134039
18-19	0.006932659856649245
20-21	0.019417051365245665
22-23	0.025148904087371367
24-25	0.03219764196702456
26-27	0.031701308351421494
28-29	0.04756797328892588
30-31	0.047355830695160056
32-33	0.05797496845479658
34-35	0.05203097313890505
36-37	0.05596962054014226
38-39	0.06031253967666907
40-41	0.04318502724791496
42-43	0.049213079063223135
44-45	0.046443217272922166
46-47	0.04438983707288691
48-49	0.037569252548793196
50-51	0.03729706959830119
52-53	0.03862596282717391
54-55	0.05803901150197116
56-57	0.044617990428446386
58-59	0.044858151855351094
60-61	0.046143015489291284
62-63	0.030804705690977246
64-65	0.03290211548594503
66-67	0.033082236556123565
68-69	0.031148937069540663
70-71	0.044145672955533795
72-73	0.03296215584267121
74-75	0.04127574390402253
76-77	0.04626709889319205
78-79	0.04076740221707423
80-81	0.03413894683450428
82-83	0.021438410041693626
84-85	0.03369064550428216
86-87	0.02770662328390651
88-89	0.02676999371897815
90-91	0.022343018083034693
92-93	0.015278269441587857
94-95	0.015398350155040213
96-97	0.0152582559893458
98-99	0.011395659706628408
100	0.005547728961498761
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2491598E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.015236579210864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.31040748783843	21.80693922216028
2	15.224605622130163	9.443894903911017
3	5.960675308517373	5.546152645965811
4	2.7800459124229167	3.448951266994596
5	1.4824613450338089	2.2989444667879364
6	0.9194208597276565	1.710963328818883
7	0.5970048016070136	1.296137161253646
8	0.41400176830372615	1.0272290230521368
9	0.31109186447672216	0.8683728997152008
>10	1.6169929734383863	9.291187730484083
>50	0.15824129964699352	3.450843299502769
>100	0.16271214185681399	11.015860306610788
>500	0.03161453316099121	6.872916276304045
>1k	0.02875856851273518	17.27514659337551
>5k	0.0016293070995249976	3.3197680568429853
>10k+	3.362062268861107E-4	1.3266928182203677
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19241	0.15403153383578308	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	16120	0.12904674005679656	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	16103	0.12891064858155057	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	15036	0.12036890716463977	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.005380896823609E-6	0.0	0.0	0.0
3	0.0	8.005380896823609E-6	0.0	0.0	0.0
4	0.0	4.803228538094165E-5	0.0	0.0	0.0
5	0.0	4.803228538094165E-5	0.0	0.0	0.0
6	8.005380896823609E-6	4.803228538094165E-5	0.0	0.0	0.0
7	8.005380896823609E-6	4.803228538094165E-5	0.0	8.005380896823609E-6	0.0
8	1.6010761793647218E-5	5.6037666277765266E-5	0.0	8.005380896823609E-6	0.0
9	1.6010761793647218E-5	1.0406995165870691E-4	0.0	8.005380896823609E-6	0.0
10-11	2.4016142690470827E-5	1.0406995165870691E-4	0.0	8.005380896823609E-6	0.0
12-13	2.8018833138882633E-5	1.0406995165870691E-4	0.0	8.005380896823609E-6	0.0
14-15	3.602421403570624E-5	1.2808609434917774E-4	0.0	8.005380896823609E-6	0.0
16-17	4.402959493252985E-5	1.2808609434917774E-4	0.0	2.4016142690470827E-5	0.0
18-19	5.6037666277765266E-5	1.4409685614282495E-4	0.0	3.2021523587294436E-5	0.0
20-21	6.404304717458887E-5	1.601076179364722E-4	0.0	6.804573762300067E-5	0.0
22-23	6.404304717458887E-5	1.7211568928170759E-4	0.0	5.683820436744762E-4	0.0
24-25	8.00538089682361E-5	1.84123760626943E-4	0.0	0.0019973425337574905	0.0
26-27	1.1207533255553053E-4	1.921291415237666E-4	0.0	0.0034543218569793874	0.0
28-29	1.2008071345235414E-4	1.921291415237666E-4	0.0	0.014557785160873733	0.0
30-31	1.3609147524600135E-4	1.921291415237666E-4	0.0	0.05889158456748288	0.0
32-33	1.3609147524600135E-4	1.921291415237666E-4	0.0	0.1473990757627647	0.0
34-35	1.3609147524600135E-4	2.2415066511106106E-4	0.0	0.26325695079204436	0.0
36-37	1.4409685614282495E-4	2.561721886983555E-4	0.0	0.44784902620145156	0.0
38-39	1.5210223703964859E-4	2.561721886983555E-4	0.0	0.7428833364634373	0.0
40-41	1.601076179364722E-4	2.641775695951791E-4	0.0	1.0615895580373305	0.0
42-43	1.64110308384884E-4	2.7618564094041454E-4	0.0	1.3581769121932998	0.0
44-45	1.681129988332958E-4	3.242179263213561E-4	0.0	1.7132996114668435	0.0
46-47	1.681129988332958E-4	3.602421403570624E-4	0.0	2.098286384175988	0.0
48-49	1.681129988332958E-4	3.642448308054742E-4	0.0	2.4868715755982542	0.0
50-51	1.761183797301194E-4	4.3229056842847486E-4	0.0	2.8803120305344443	0.0
52-53	1.8012107017853122E-4	4.3229056842847486E-4	0.0	3.2505008566558096	0.0
54-55	2.0013452242059022E-4	4.3629325887688665E-4	0.0	3.6317050868912046	0.0
56-57	2.0013452242059022E-4	4.483013302221221E-4	0.0	4.0659529709489535	0.0
58-59	2.0013452242059022E-4	4.563067111189457E-4	0.0	4.550702800394313	0.0
60-61	2.0013452242059022E-4	4.563067111189457E-4	0.0	5.041424643988703	0.0
62-63	2.04137212869002E-4	4.563067111189457E-4	0.0	5.527711506566254	0.0
64-65	2.0813990331741382E-4	5.043389964998874E-4	0.0	6.0262025723210115	0.0
66-67	2.0813990331741382E-4	5.12344377396711E-4	0.0	6.5640360824932085	0.0
68-69	2.0813990331741382E-4	5.203497582935345E-4	0.0	7.14782848439407	0.0
70-71	2.0813990331741382E-4	5.3235782963877E-4	0.0	7.76721280976221	0.0
72-73	2.0813990331741382E-4	5.363605200871818E-4	0.0	8.395979441541428	0.0
74-75	2.0813990331741382E-4	5.523712818808291E-4	0.0	9.023365145115942	0.0
76-77	2.0813990331741382E-4	5.803901150197117E-4	0.0	9.704707115935047	0.0
78-79	2.0813990331741382E-4	5.843928054681235E-4	0.0	10.411165969317937	0.0
80-81	2.2415066511106106E-4	5.843928054681235E-4	0.0	11.172697840580526	0.0
82-83	2.2415066511106106E-4	5.843928054681235E-4	0.0	11.957833577417396	0.0
84-85	2.2815335555947285E-4	5.883954959165353E-4	0.0	12.733863193484133	0.0
86-87	2.4016142690470827E-4	6.084089481585942E-4	0.0	13.539640804963465	0.0
88	2.4016142690470827E-4	6.404304717458888E-4	0.0	14.163151904183916	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	30730	0.0	39.81971	1
CAACGCA	34880	0.0	31.984573	5
ATCAACG	35180	0.0	31.711823	3
TATCAAC	39275	0.0	30.95184	2
GTACAAG	4155	0.0	29.043459	1
GGTATCA	17810	0.0	28.658398	1
AACGCAG	41200	0.0	28.18367	6
CGCAGAG	40750	0.0	26.91633	8
ACGCAGA	41080	0.0	26.905846	7
GCAGAGT	41080	0.0	25.32883	9
TCAACGC	45495	0.0	24.831476	4
GTACATG	54475	0.0	23.695385	1
TACATGG	54415	0.0	23.24607	2
GTATAAG	6725	0.0	23.04135	1
AGAGTAC	37665	0.0	21.855186	10-11
CTATTGA	8355	0.0	21.636654	9
GTGATCG	5700	0.0	21.252766	8
CGTTAAC	2420	0.0	21.149366	1
ACGTTAA	1890	0.0	21.116781	8
ACATGGG	58040	0.0	20.791046	3
>>END_MODULE