FastQCFastQC Report
Fri 27 May 2016
ERR765438_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765438_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6882362
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA469150.681670042930029No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT426830.6201795255756672No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG181570.2638193108703088No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG165260.24012105146459894No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG131990.19178008945184807No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC131340.1908356462505169No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC130540.1896732546181093No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT128250.18634590857034258No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA124550.18096984727045745No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC122390.17783138986295693No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC89680.13030410199289139No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC87660.12736906312106222No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT83340.12109214830606121No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT76750.11151694723410363No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC71260.10354003465670651No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA17950.044.297391
TACATGA37250.033.0800442
GTACAAG48850.029.6034471
GTACTAG35400.028.372461
GTACAAA55700.026.964591
GTAACAA21000.026.3496061
GTATAAG37950.026.0984251
TACAAGA29800.025.746262
GTGATCG31000.025.1974288
TGATCGC32500.023.749389
TACATAG18800.023.0023162
TCTAACG21100.022.919092
TACATAA18700.022.8766632
ATAAGGT37500.022.5676613
ACGTTAA8600.022.1663428
CTAACGC22200.021.7834593
CGCCTAC22250.021.7324547
TATTCGC5450.021.329039
TAGTACT46800.020.5658054
GGAAACA63950.020.5047761
Produced by FastQC (version 0.11.5)