FastQCFastQC Report
Fri 27 May 2016
ERR765438_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765438_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6882362
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT478380.6950811363889315No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA443040.6437324860273261No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327020.4751566395374146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234880.34127818327486986No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG181170.263238115054105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166340.2416902801683492No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG152050.22092705963446851No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT145730.21174416573844854No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC140680.20440656855887557No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG139890.20325870682187308No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA139030.20200913581703492No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC130980.1903125700159335No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC118440.17209208117794444No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC98050.14246562444695585No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC90940.13213486881393335No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT82780.12027847416337589No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG78280.11374002123108316No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG73580.10691097039068855No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT72130.10480413555694978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG7050.059.3667032
TCACGCA9350.043.765534
ATCACGC14100.029.0218223
TAACGCA4650.026.000494
GTACTAG34450.024.7145881
AGTACTT614100.022.54769912-13
GTATCAA1034000.021.9354021
CTAACGC18100.021.5805173
TCAACGA8200.021.5491853
GTGATCG27650.020.1812118
TCTAACG19650.020.1161982
GACGTTA11650.019.9570247
TACTTTT711750.019.71535714-15
GTATAAG35500.019.6586841
CTATTGA24650.019.6191759
GTTAATA37550.019.5766621
TAGTACT47300.019.5637934
GAGTACT596800.019.29741112-13
TACGGGC30700.019.2365174
ATACGGG31700.019.069763
Produced by FastQC (version 0.11.5)