##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765438_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6882362 Sequences flagged as poor quality 0 Sequence length 99 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29328128918531 33.0 33.0 33.0 33.0 33.0 2 32.04088857865948 33.0 33.0 33.0 27.0 33.0 3 32.10993914589206 33.0 33.0 33.0 27.0 33.0 4 36.07316790369353 37.0 37.0 37.0 33.0 37.0 5 36.057556984070295 37.0 37.0 37.0 33.0 37.0 6 36.089582035934754 37.0 37.0 37.0 33.0 37.0 7 35.99877135204454 37.0 37.0 37.0 33.0 37.0 8 35.97243301064373 37.0 37.0 37.0 33.0 37.0 9 35.68949104972973 37.0 37.0 37.0 33.0 37.0 10-11 35.87284082121806 37.0 37.0 37.0 33.0 37.0 12-13 35.788685991815015 37.0 37.0 37.0 33.0 37.0 14-15 37.07995794176476 37.0 37.0 40.0 33.0 40.0 16-17 37.25070818710205 37.0 37.0 40.0 33.0 40.0 18-19 37.36890409135701 37.0 37.0 40.0 33.0 40.0 20-21 37.40642885393125 37.0 37.0 40.0 33.0 40.0 22-23 37.32215400759216 37.0 37.0 40.0 33.0 40.0 24-25 37.253243290602846 37.0 37.0 40.0 33.0 40.0 26-27 37.08586608783438 37.0 37.0 40.0 33.0 40.0 28-29 36.983841085371566 37.0 37.0 40.0 33.0 40.0 30-31 36.79034014775741 37.0 37.0 40.0 33.0 40.0 32-33 36.51293567528125 37.0 37.0 40.0 33.0 40.0 34-35 36.35519992990778 37.0 37.0 40.0 33.0 40.0 36-37 36.1203644184947 37.0 37.0 40.0 33.0 40.0 38-39 35.89361414002925 37.0 37.0 40.0 33.0 40.0 40-41 35.66978393464336 37.0 37.0 40.0 27.0 40.0 42-43 35.38843321522466 37.0 37.0 40.0 27.0 40.0 44-45 35.01778168599675 37.0 33.0 40.0 24.5 40.0 46-47 34.56060731766216 37.0 33.0 40.0 22.0 40.0 48-49 34.136569392891566 37.0 33.0 40.0 22.0 40.0 50-51 33.74381091840272 37.0 33.0 40.0 15.0 40.0 52-53 33.57005116847966 37.0 33.0 40.0 15.0 40.0 54-55 33.44417650510101 37.0 33.0 40.0 15.0 40.0 56-57 33.26915926538011 37.0 33.0 38.5 15.0 40.0 58-59 33.07962644220109 37.0 33.0 37.0 15.0 40.0 60-61 32.89657642245496 37.0 33.0 37.0 15.0 40.0 62-63 32.56747080435467 37.0 33.0 37.0 15.0 40.0 64-65 32.42759687735112 37.0 33.0 37.0 15.0 40.0 66-67 32.24761731510199 37.0 33.0 37.0 15.0 40.0 68-69 32.004647459694795 37.0 33.0 37.0 15.0 38.5 70-71 31.803394604933594 37.0 33.0 37.0 15.0 37.0 72-73 31.597832401143677 35.0 33.0 37.0 15.0 37.0 74-75 31.39770474148265 33.0 33.0 37.0 15.0 37.0 76-77 31.236706453394923 33.0 33.0 37.0 15.0 37.0 78-79 31.0549405858047 33.0 33.0 37.0 15.0 37.0 80-81 30.936411511048096 33.0 33.0 37.0 15.0 37.0 82-83 30.797740586734612 33.0 30.0 37.0 15.0 37.0 84-85 30.648919949284853 33.0 27.0 37.0 15.0 37.0 86-87 30.55414115095951 33.0 27.0 37.0 15.0 37.0 88-89 30.453516394516882 33.0 27.0 37.0 15.0 37.0 90-91 30.396498832813503 33.0 27.0 37.0 15.0 37.0 92-93 30.282287389126 33.0 27.0 37.0 15.0 37.0 94-95 30.228522853055388 33.0 27.0 37.0 15.0 37.0 96-97 30.18905725970241 33.0 27.0 37.0 15.0 37.0 98-99 29.381065904408977 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.185228581375803 1101 2 0.39002076682505304 1101 3 0.30391105592215695 1101 4 0.2566637602315538 1101 5 0.14086111434406945 1101 6 0.22855901593810302 1101 7 0.2459262070630075 1101 8 0.16577032757329846 1101 9 0.3916360798262204 1101 10-11 0.3728360317254129 1101 12-13 0.3036944681865492 1101 14-15 0.6110811297771548 1101 16-17 0.5292854016304886 1101 18-19 0.34966662697645745 1101 20-21 0.4225241887538189 1101 22-23 0.2951558157663996 1101 24-25 0.4021485266985749 1101 26-27 0.3067700991921214 1101 28-29 0.31511657372877977 1101 30-31 0.3670417849558021 1101 32-33 0.4822953168673365 1101 34-35 0.5294427924277016 1101 36-37 0.4875145122605957 1101 38-39 0.2367950196186399 1101 40-41 0.1991089503696699 1101 42-43 0.35463561535615895 1101 44-45 0.4071812755884281 1101 46-47 0.5124884028224415 1101 48-49 0.5251137107973847 1101 50-51 0.45425557434789 1101 52-53 0.43910452029629 1101 54-55 0.40621606048614467 1101 56-57 0.4958753475567619 1101 58-59 0.5250969758293778 1101 60-61 0.4071461826553602 1101 62-63 0.36656554372861194 1101 64-65 0.32710177394027085 1101 66-67 0.32056606466074555 1101 68-69 0.22924109022583394 1101 70-71 0.2562706522735603 1101 72-73 0.35914051828718385 1101 74-75 0.23007706301852338 1101 76-77 0.25315022601971293 1101 78-79 0.4504564280174357 1101 80-81 0.3999646208976557 1101 82-83 0.34496263307107 1101 84-85 0.41114383698957724 1101 86-87 0.22477155105867652 1101 88-89 0.3581375102584978 1101 90-91 0.2934128073297728 1101 92-93 0.3923817344339504 1101 94-95 0.43712188101587657 1101 96-97 0.271683011189225 1101 98-99 0.3939763187785985 1102 1 0.127021245756211 1102 2 0.36657879971767215 1102 3 0.23492703589416664 1102 4 0.17900895431331776 1102 5 0.13355046965069306 1102 6 0.20077268748545407 1102 7 0.1653205159350648 1102 8 0.13324807456310594 1102 9 0.4178174754538517 1102 10-11 0.2870570830613772 1102 12-13 0.2951986330275673 1102 14-15 0.5126944337244197 1102 16-17 0.3514669332565319 1102 18-19 0.2803046832742453 1102 20-21 0.3520522405201447 1102 22-23 0.23975861732474613 1102 24-25 0.3798787613393486 1102 26-27 0.3115792110645188 1102 28-29 0.29045832908682456 1102 30-31 0.26029168419631077 1102 32-33 0.35827609773813407 1102 34-35 0.4870149899182792 1102 36-37 0.47096178581756476 1102 38-39 0.3665316930814484 1102 40-41 0.3917552490472289 1102 42-43 0.39358755122110267 1102 44-45 0.419993366797506 1102 46-47 0.5102710616623867 1102 48-49 0.523568768861054 1102 50-51 0.4633337093116978 1102 52-53 0.5755260859873133 1102 54-55 0.48303563740604716 1102 56-57 0.5412813374307461 1102 58-59 0.5325196770592484 1102 60-61 0.43880219806597864 1102 62-63 0.5045616752325159 1102 64-65 0.3941037544565802 1102 66-67 0.4349800579941103 1102 68-69 0.42347510036919545 1102 70-71 0.4119961984765901 1102 72-73 0.5140503831885468 1102 74-75 0.3313573074479095 1102 76-77 0.407593143297472 1102 78-79 0.5878257777441753 1102 80-81 0.4844267010109391 1102 82-83 0.48465565696570323 1102 84-85 0.48822141119528695 1102 86-87 0.3506163450427131 1102 88-89 0.3497939671193464 1102 90-91 0.3510847853110306 1102 92-93 0.5024156035994203 1102 94-95 0.509248358296702 1102 96-97 0.3563739539811408 1102 98-99 0.36170628875232325 1103 1 0.09580563210852944 1103 2 0.2806811127738911 1103 3 0.21777377271064324 1103 4 0.18625456531924556 1103 5 0.09460598926251862 1103 6 0.15283519054508332 1103 7 0.1454941858957639 1103 8 0.10480007301245564 1103 9 0.370241813901778 1103 10-11 0.268272569913691 1103 12-13 0.31148574603157186 1103 14-15 0.4364183375839801 1103 16-17 0.3447847347086679 1103 18-19 0.1953544471481976 1103 20-21 0.29305497632314115 1103 22-23 0.12344072336713197 1103 24-25 0.23209396461106024 1103 26-27 0.14817060648832836 1103 28-29 0.041918157227279096 1103 30-31 0.06513798505724822 1103 32-33 0.15272265635751126 1103 34-35 0.20110929832783597 1103 36-37 0.18076959766911216 1103 38-39 0.05546787112949403 1103 40-41 -0.042505032844225354 1103 42-43 0.01752459258020167 1103 44-45 0.1407745321064695 1103 46-47 0.24270261256157255 1103 48-49 0.17378330937889785 1103 50-51 0.16019345389951667 1103 52-53 0.07188860254237994 1103 54-55 0.035841566511784606 1103 56-57 0.11975361202496515 1103 58-59 0.01933184251242892 1103 60-61 0.02683881259880394 1103 62-63 0.14126155236228755 1103 64-65 -0.024548734393135874 1103 66-67 0.06868584169311731 1103 68-69 0.08696701777094518 1103 70-71 -0.04184641506479991 1103 72-73 0.032295216663236204 1103 74-75 0.012814088640560328 1103 76-77 -0.00900809971610883 1103 78-79 0.2654353118516859 1103 80-81 0.042052742221418526 1103 82-83 0.09377364137803212 1103 84-85 0.15198847454401943 1103 86-87 0.04624070307225381 1103 88-89 0.07063259002462985 1103 90-91 -0.07619140101950705 1103 92-93 -0.08028759810488495 1103 94-95 0.15944498207786495 1103 96-97 0.06075265006609598 1103 98-99 0.04426774701812519 1104 1 0.09869909388583409 1104 2 0.2960226903795373 1104 3 0.2093640429985868 1104 4 0.18971589322720916 1104 5 0.054787951546401814 1104 6 0.15155340638323622 1104 7 0.12940294329028035 1104 8 0.0285916212034536 1104 9 0.37789881458811436 1104 10-11 0.25805966096837096 1104 12-13 0.24287761043004252 1104 14-15 0.3940392848990797 1104 16-17 0.20935683328041677 1104 18-19 0.1715801596363491 1104 20-21 0.26458915451055276 1104 22-23 0.1712625073655616 1104 24-25 0.25074775419827944 1104 26-27 0.037598593709297745 1104 28-29 0.08174436567909282 1104 30-31 0.16420866600635264 1104 32-33 0.15342993566593321 1104 34-35 0.21181091940179897 1104 36-37 0.1747666602896345 1104 38-39 0.007750264011150421 1104 40-41 -0.033793496582511295 1104 42-43 0.11823861274557856 1104 44-45 0.0521751596189759 1104 46-47 0.182069625667431 1104 48-49 0.2223629291128617 1104 50-51 0.15289085887386733 1104 52-53 0.23430801866160067 1104 54-55 0.15762893537146994 1104 56-57 0.19345415864718518 1104 58-59 0.24934060562646465 1104 60-61 0.15618309063259517 1104 62-63 0.16947714539428915 1104 64-65 0.0655515001069702 1104 66-67 0.09959828817480343 1104 68-69 0.09657018556517727 1104 70-71 0.18688005321640233 1104 72-73 0.11428088927819857 1104 74-75 0.09072409615763632 1104 76-77 0.06925631346165417 1104 78-79 0.2682465556010776 1104 80-81 0.1687277430384917 1104 82-83 0.12713066984487753 1104 84-85 0.13111735155810678 1104 86-87 0.1842296194692885 1104 88-89 0.0663919781999418 1104 90-91 0.1000462611695987 1104 92-93 0.14185325720772823 1104 94-95 0.1018933838819649 1104 96-97 0.11654345472014782 1104 98-99 0.07291434635792626 1105 1 0.08091825147429432 1105 2 0.29344282984532555 1105 3 0.22840890011499226 1105 4 0.18666127037239022 1105 5 -0.04018409521852817 1105 6 0.046462289242661825 1105 7 0.07634837073949541 1105 8 0.005769290177589426 1105 9 0.2884364073703196 1105 10-11 0.1711379220497733 1105 12-13 0.20081383231223526 1105 14-15 0.32190621440673794 1105 16-17 0.3006468041500696 1105 18-19 0.18226749496248829 1105 20-21 0.28106318785378903 1105 22-23 0.15343165735092867 1105 24-25 0.27194336044241396 1105 26-27 0.08966698255145644 1105 28-29 0.13697094081349093 1105 30-31 0.18143363981950955 1105 32-33 0.1953022530234776 1105 34-35 0.22479723009217878 1105 36-37 0.13761334673392867 1105 38-39 0.16622504091992596 1105 40-41 0.06807362580520504 1105 42-43 0.24777527339396954 1105 44-45 0.16364538566519826 1105 46-47 0.22039945185117205 1105 48-49 0.25176476775418166 1105 50-51 0.1573763082153974 1105 52-53 0.0862528068902293 1105 54-55 0.09271627288224238 1105 56-57 0.16971127271605724 1105 58-59 0.2325874836860251 1105 60-61 0.15656855430359684 1105 62-63 0.19141868865317946 1105 64-65 0.03584949008698146 1105 66-67 0.11621524786487925 1105 68-69 0.05479928474058937 1105 70-71 0.14631904534195073 1105 72-73 0.2030982100937777 1105 74-75 0.1640867320719579 1105 76-77 0.1426660734797771 1105 78-79 0.28462176845901865 1105 80-81 0.21204875379895327 1105 82-83 0.15947377658199358 1105 84-85 0.16248040988261891 1105 86-87 0.19279380102546995 1105 88-89 0.10461171040424233 1105 90-91 0.07636604867959562 1105 92-93 0.06176520502030414 1105 94-95 0.29127802465077224 1105 96-97 0.1333682329655801 1105 98-99 0.11953275085183179 1106 1 0.11096969749922891 1106 2 0.2839057548612587 1106 3 0.22562687048015562 1106 4 0.1433705450541325 1106 5 0.07500327034263421 1106 6 0.12192730340044022 1106 7 0.12869122392562105 1106 8 0.039810921006001365 1106 9 0.3102195917945494 1106 10-11 0.18932597887032898 1106 12-13 0.2056883225540176 1106 14-15 0.3788647293916867 1106 16-17 0.2363251217605864 1106 18-19 0.21293098361307017 1106 20-21 0.308730688159379 1106 22-23 0.19466399473903806 1106 24-25 0.2706729098005596 1106 26-27 0.09031739012616669 1106 28-29 0.17376154635523022 1106 30-31 0.09786551476793193 1106 32-33 0.14403679557752724 1106 34-35 0.2696702993705884 1106 36-37 0.1892823131735284 1106 38-39 0.09432949808271474 1106 40-41 0.07910022546781903 1106 42-43 0.13337597787177913 1106 44-45 0.1607597662428688 1106 46-47 0.29743050818451167 1106 48-49 0.3631920851059576 1106 50-51 0.21805699321320304 1106 52-53 0.230819793790495 1106 54-55 0.23522118138321702 1106 56-57 0.30239179834222796 1106 58-59 0.26254702198408353 1106 60-61 0.27178536396753117 1106 62-63 0.265388009650394 1106 64-65 0.15421096001730916 1106 66-67 0.147997293819202 1106 68-69 0.12739005178504215 1106 70-71 0.1571236239025886 1106 72-73 0.282409011705802 1106 74-75 0.248055852704141 1106 76-77 0.1799560074623301 1106 78-79 0.2767561396974152 1106 80-81 0.2697499380873225 1106 82-83 0.20924210715995528 1106 84-85 0.1979561464020243 1106 86-87 0.19193598377459864 1106 88-89 0.05099475150728949 1106 90-91 0.07677253128867179 1106 92-93 -0.03319184057382074 1106 94-95 0.18369160067785373 1106 96-97 0.21255901025484292 1106 98-99 0.07444213859468363 1107 1 0.06551662746437614 1107 2 0.2189099376288013 1107 3 0.23701459945429804 1107 4 0.1559819975247123 1107 5 0.06143343820368585 1107 6 0.08364912839585514 1107 7 0.08764917642216119 1107 8 -0.022628471519453797 1107 9 0.2914428096760133 1107 10-11 0.20855674810108837 1107 12-13 0.12633920404587684 1107 14-15 0.29669951231068126 1107 16-17 0.19369160949572972 1107 18-19 0.12519991834784605 1107 20-21 0.22251717007475236 1107 22-23 0.07696385001455042 1107 24-25 0.13199728605009398 1107 26-27 -0.07787231162030395 1107 28-29 0.009236096703816088 1107 30-31 -0.018647735205505 1107 32-33 -0.09160233452750788 1107 34-35 0.016469683727123652 1107 36-37 0.0018232968568341335 1107 38-39 -0.0402625824677898 1107 40-41 -0.0756564774938866 1107 42-43 -0.12010899417946064 1107 44-45 -0.14193928142194778 1107 46-47 -0.11780774414794593 1107 48-49 -0.02205409581036122 1107 50-51 -0.1341402784009773 1107 52-53 -0.17567724945425311 1107 54-55 -0.14721394670643662 1107 56-57 -0.03792284502703325 1107 58-59 -0.1198384029046764 1107 60-61 -0.029878031286990847 1107 62-63 -0.014317338538866409 1107 64-65 -0.13976862208463103 1107 66-67 -0.2234087434109142 1107 68-69 -0.19950280267888587 1107 70-71 -0.18158410535869152 1107 72-73 -0.04021706614621223 1107 74-75 -0.21341243654966036 1107 76-77 -0.1539424494444468 1107 78-79 -0.1059039402417703 1107 80-81 -0.18000199846486709 1107 82-83 -0.18313441536794883 1107 84-85 -0.20097662105867542 1107 86-87 -0.09708339574011049 1107 88-89 -0.09603314273012842 1107 90-91 -0.19040285063010032 1107 92-93 -0.20149647609564525 1107 94-95 0.05889274128698574 1107 96-97 -0.16298425275326878 1107 98-99 -0.17695506493834046 1108 1 0.08757528718464158 1108 2 0.24712696530845335 1108 3 0.16518858907707568 1108 4 0.12687912892767628 1108 5 -0.07137554264643597 1108 6 0.05800730337848137 1108 7 0.1000701878507968 1108 8 -0.005563353883225375 1108 9 0.2847134309943584 1108 10-11 0.1507457754715773 1108 12-13 0.16024333924480771 1108 14-15 0.21937462860478973 1108 16-17 0.26351989739544024 1108 18-19 0.07439761500184972 1108 20-21 0.25655736774892546 1108 22-23 0.10947837523139725 1108 24-25 0.16318555447281113 1108 26-27 -0.030154260848640035 1108 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0.06686533054329402 2216 1 -0.2312058941246704 2216 2 0.03282326919212153 2216 3 -0.15443006121486746 2216 4 -0.33857866908825685 2216 5 -0.3192006217308929 2216 6 -0.3117043990226449 2216 7 -0.338667719965315 2216 8 -0.23174673539293877 2216 9 0.05167570259372667 2216 10-11 -0.22983025249520495 2216 12-13 -0.18446375645393687 2216 14-15 -0.5154483035268456 2216 16-17 -0.66264538518395 2216 18-19 -0.6416263811632774 2216 20-21 -0.8669052232047605 2216 22-23 -0.7782033518000162 2216 24-25 -0.6122903457522426 2216 26-27 -0.738112331004281 2216 28-29 -0.7841697177634401 2216 30-31 -0.4835216729891414 2216 32-33 -0.5716728484252798 2216 34-35 -0.5769211983073177 2216 36-37 -0.5464633266333863 2216 38-39 -0.5940572848847836 2216 40-41 -0.5438129319343901 2216 42-43 -0.6415889838908342 2216 44-45 -0.4885120477802545 2216 46-47 -0.728365686793488 2216 48-49 -0.8955872784603685 2216 50-51 -0.4599857839044361 2216 52-53 -0.512674846012068 2216 54-55 -0.5078081680372435 2216 56-57 -0.581303451691511 2216 58-59 -0.465814907774039 2216 60-61 -0.5237899160119213 2216 62-63 -0.5431804716052362 2216 64-65 -0.5953803330895191 2216 66-67 -0.5560067497239665 2216 68-69 -0.5074314273073597 2216 70-71 -0.5361709062150588 2216 72-73 -0.4349478647115035 2216 74-75 -0.49621452496888807 2216 76-77 -0.35185457713216195 2216 78-79 -0.259775575348133 2216 80-81 -0.3386616328009069 2216 82-83 -0.22025519206659183 2216 84-85 -0.2989991178211042 2216 86-87 -0.4735004272652752 2216 88-89 -0.29940231224489366 2216 90-91 -0.2451086303268326 2216 92-93 -0.39581278778142703 2216 94-95 -0.41064515378027266 2216 96-97 -0.4023836010296442 2216 98-99 -0.3786687563384845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 5.0 9 26.0 10 111.0 11 284.0 12 462.0 13 1121.0 14 3459.0 15 9571.0 16 21220.0 17 39075.0 18 60740.0 19 79107.0 20 96327.0 21 115858.0 22 126971.0 23 102426.0 24 67988.0 25 54891.0 26 61673.0 27 78967.0 28 103027.0 29 135515.0 30 177674.0 31 233329.0 32 307709.0 33 404212.0 34 534122.0 35 742764.0 36 1183008.0 37 1623264.0 38 516771.0 39 682.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.038524190140244 18.887488913224914 13.44169988156177 23.63228701507307 2 15.199649629402302 22.035610742218378 37.343385272810124 25.421354355569196 3 17.92749349714531 27.927810248865143 30.199748284092003 23.94494796989754 4 12.577992846060699 18.040129246325606 36.528752774120285 32.853125133493414 5 12.489569140668648 38.35702504844728 33.40326228894356 15.750143521940513 6 29.599169587417805 37.80718015123296 17.43731294575903 15.156337315590198 7 24.438630226076455 33.54365841262055 23.218249781107126 18.79946158019587 8 27.548115022139203 31.52202688553726 20.45030470643654 20.479553385886998 9 27.181449756172128 13.209482732691388 20.637699867874804 38.97136764326168 10-11 24.97151414005831 25.723414432428864 26.814108295960022 22.490963131552803 12-13 27.116925846097605 23.766913161498916 27.358761425220003 21.757399567183473 14-15 24.34653560944203 23.122040683448695 27.53893767306006 24.992486034049218 16-17 21.64287546689317 25.634695359813335 28.642195365443918 24.08023380784958 18-19 22.315899686764514 23.696573937842853 32.88529432192029 21.10223205347234 20-21 23.43551530709951 23.044377787742057 32.226298180769916 21.293808724388516 22-23 22.59573094237124 22.937110834913945 31.568914858009506 22.898243364705316 24-25 22.231764327421313 23.75654608112738 31.543770874010985 22.467918717440323 26-27 22.723208979707838 23.048656841938858 31.538670880723796 22.689463297629505 28-29 22.009420602984846 22.862390847793243 32.663727946888 22.464460602333908 30-31 22.80724989473091 23.007348349302173 32.141915813204825 22.043485942762093 32-33 22.009268039083093 23.359952586045313 31.57397852655818 23.056800848313415 34-35 22.134987959075676 23.625377450357885 32.03500484281414 22.204629747752296 36-37 23.020381665480542 23.22847156252461 31.02060746005514 22.730539311939708 38-39 23.219986103608033 22.74389955076469 30.909933246754527 23.126181098872742 40-41 22.554800227015086 23.17275667859377 31.110344094077004 23.162099000314136 42-43 23.71171408885496 23.142251163190778 30.058524965702183 23.08750978225208 44-45 23.517348480137258 23.495306532727568 29.38310735372836 23.604237633406814 46-47 23.12215631784553 23.677053023366106 29.304939205464635 23.895851453323726 48-49 24.219068976610064 24.2747765955932 27.836250112969935 23.6699043148268 50-51 23.145302441225844 25.01908501761459 26.91312226819804 24.92249027296152 52-53 22.80483703721409 25.225992823530497 26.111198734560787 25.85797140469463 54-55 23.15050414378087 25.39840246706 26.00623158154134 25.44486180761779 56-57 23.72063746961501 24.32811567695094 25.80640685107728 26.144840002356766 58-59 22.796454182444922 24.41891315801174 27.215707340009143 25.5689253195342 60-61 23.27307107647055 24.785182761383375 26.617373512175035 25.324372649971043 62-63 22.495118681638658 24.666001294323085 26.61581154841899 26.22306847561927 64-65 22.09356322727575 24.66132266800264 26.987319179084157 26.25779492563745 66-67 23.614080456680426 24.297472292215957 26.57030391601023 25.51814333509339 68-69 23.36772775317793 23.80084796156268 26.727702249962746 26.103722035296638 70-71 22.593317366880804 24.138306307426813 26.882915086960885 26.385461238731494 72-73 23.590309547797688 23.881030960010534 27.036016123534335 25.492643368657447 74-75 23.770422131239247 23.76547470185381 27.013538375342648 25.45056479156429 76-77 23.001485536506216 24.309720994042454 27.126769850234556 25.562023619216774 78-79 23.265975627023707 24.60030464948999 26.74964770316704 25.384072020319266 80-81 22.49585931237342 24.589640020626792 27.044760110375716 25.869740556624077 82-83 22.719126079099006 24.465561387209796 26.45195065298803 26.363361880703167 84-85 22.88523184337005 24.697829030207945 25.842959146874282 26.57397997954772 86-87 22.65296420037191 24.170677159963397 26.29924145228048 26.877117187384215 88-89 22.863909221863075 23.59307749287236 26.53562832062597 27.007384964638593 90-91 23.09926230042946 24.08134819242464 26.12811541886692 26.69127408827897 92-93 22.669164861614263 24.192593975635333 26.121410676150898 27.01683048659951 94-95 21.941571421368707 24.396449625903603 26.67896581921466 26.98301313351303 96-97 22.937907450909737 24.178995611599095 25.9486170961289 26.93447984136227 98-99 22.73972947078343 24.16731348917712 26.031404625330662 27.06155241470879 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 60.0 1 79.0 2 242.0 3 431.0 4 331.0 5 278.0 6 554.5 7 1402.0 8 2497.0 9 2434.5 10 1502.0 11 1080.5 12 1169.0 13 1375.5 14 1772.5 15 2404.0 16 3128.5 17 4151.5 18 5477.5 19 6887.5 20 8703.0 21 11104.5 22 14175.5 23 17926.5 24 21721.5 25 26790.0 26 33757.0 27 40668.5 28 49034.5 29 58050.5 30 65130.5 31 73635.0 32 82915.0 33 90658.0 34 96780.5 35 102776.5 36 107002.0 37 113696.0 38 124481.5 39 122519.0 40 120750.5 41 129765.5 42 140347.0 43 160592.0 44 191451.5 45 230921.0 46 269818.5 47 313034.5 48 352147.5 49 392533.5 50 442566.5 51 405730.0 52 330047.5 53 303817.0 54 272127.5 55 248864.5 56 227113.0 57 197848.0 58 184742.0 59 157798.5 60 118420.5 61 85441.5 62 65823.0 63 52591.0 64 37675.0 65 29182.5 66 23195.5 67 17925.5 68 13625.0 69 11065.0 70 10232.5 71 9422.5 72 9589.0 73 8285.0 74 5387.0 75 4059.0 76 3213.0 77 2218.0 78 1213.0 79 760.5 80 580.5 81 484.0 82 413.5 83 312.0 84 191.5 85 132.5 86 69.5 87 25.0 88 21.5 89 14.0 90 8.0 91 7.5 92 4.5 93 2.5 94 2.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.053339246032103514 2 0.0073521270749780385 3 0.0 4 0.0 5 0.002019655461308196 6 0.0 7 0.0 8 0.0 9 0.0036760635374890193 10-11 0.0 12-13 0.0 14-15 0.0025790564344043516 16-17 0.004366233569231028 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 4.3589686215284815E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.2350411094330698E-4 54-55 0.0 56-57 8.354689857929589E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0013440153249712817 70-71 2.1794843107642408E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.850422282350158E-4 80-81 7.410246656598418E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.0512780350699367E-4 92-93 8.645287766031488E-4 94-95 6.538452932292721E-4 96-97 2.106834833738766E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6882362.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.77329585767025 #Duplication Level Percentage of deduplicated Percentage of total 1 69.11065932802667 17.12098810451863 2 14.407073277650506 7.1382137750074195 3 5.7199870720889985 4.251087961167294 4 2.942070426623645 2.9153912445139873 5 1.7427424580964683 2.158673725907365 6 1.0661035584470915 1.5846539320994908 7 0.7371601129994693 1.278331990166664 8 0.522319738646591 1.03516651342344 9 0.41150288027557225 0.917485433941519 >10 2.6528208774290687 12.874842603429137 >50 0.3150722852814156 5.486843423076121 >100 0.2899105319077432 14.984505619992117 >500 0.050536925218213756 8.691911904754143 >1k 0.030011890570278695 13.444741244036374 >5k 0.0012529815148625014 1.9819347611345766 >10k+ 7.756552234863105E-4 4.13522776283173 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 47838 0.6950811363889315 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 44304 0.6437324860273261 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32702 0.4751566395374146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23488 0.34127818327486986 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 18117 0.263238115054105 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16634 0.2416902801683492 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 15205 0.22092705963446851 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 14573 0.21174416573844854 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 14068 0.20440656855887557 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 13989 0.20325870682187308 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 13903 0.20200913581703492 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 13098 0.1903125700159335 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 11844 0.17209208117794444 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 9805 0.14246562444695585 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 9094 0.13213486881393335 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 8278 0.12027847416337589 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 7828 0.11374002123108316 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 7358 0.10691097039068855 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 7213 0.10480413555694978 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.4529895405094936E-5 0.0 4 0.0 0.0 0.0 1.4529895405094936E-5 0.0 5 0.0 0.0 0.0 1.4529895405094936E-5 0.0 6 0.0 0.0 0.0 1.4529895405094936E-5 1.4529895405094936E-5 7 0.0 0.0 0.0 2.9059790810189872E-5 1.4529895405094936E-5 8 0.0 0.0 0.0 4.358968621528481E-5 1.4529895405094936E-5 9 0.0 0.0 0.0 5.8119581620379744E-5 1.4529895405094936E-5 10-11 0.0 0.0 0.0 1.3076905864585443E-4 1.4529895405094936E-5 12-13 1.4529895405094936E-5 0.0 0.0 1.7435874486113924E-4 1.4529895405094936E-5 14-15 3.632473851273734E-5 0.0 0.0 1.8888864026623417E-4 1.4529895405094936E-5 16-17 5.0854633917832274E-5 0.0 0.0 2.1794843107642405E-4 1.4529895405094936E-5 18-19 5.8119581620379744E-5 0.0 0.0 2.6880306499425636E-4 1.4529895405094936E-5 20-21 6.538452932292721E-5 0.0 0.0 7.410246656598418E-4 1.4529895405094936E-5 22-23 9.44443201331171E-5 0.0 0.0 0.0040102511318062025 1.4529895405094936E-5 24-25 1.5982884945604428E-4 0.0 0.0 0.011304258625163861 1.4529895405094936E-5 26-27 2.3247832648151898E-4 0.0 0.0 0.018946983608243796 1.4529895405094936E-5 28-29 2.470082218866139E-4 0.0 0.0 0.0420422523546422 1.4529895405094936E-5 30-31 2.470082218866139E-4 0.0 0.0 0.10615541582962361 1.4529895405094936E-5 32-33 2.6880306499425636E-4 0.0 0.0 0.20558349008668825 1.4529895405094936E-5 34-35 2.905979081018987E-4 0.0 0.0 0.3266320487065342 1.4529895405094936E-5 36-37 3.0512780350699367E-4 0.0 0.0 0.5089749710927731 1.4529895405094936E-5 38-39 3.0512780350699367E-4 0.0 0.0 0.7800664946133319 1.4529895405094936E-5 40-41 3.341875943171835E-4 0.0 0.0 1.1261831330580985 1.4529895405094936E-5 42-43 3.5598243742482593E-4 0.0 0.0 1.411579338604973 1.4529895405094936E-5 44-45 3.7777728053246833E-4 0.0 0.0 1.7132780867963644 1.4529895405094936E-5 46-47 3.7777728053246833E-4 0.0 0.0 2.0318968981869885 1.4529895405094936E-5 48-49 3.7777728053246833E-4 0.0 0.0 2.369070386009919 1.4529895405094936E-5 50-51 3.7777728053246833E-4 0.0 0.0 2.7675237077038375 1.4529895405094936E-5 52-53 3.7777728053246833E-4 0.0 0.0 3.165286859366014 1.4529895405094936E-5 54-55 3.7777728053246833E-4 0.0 0.0 3.5229329698147236 2.9059790810189872E-5 56-57 3.7777728053246833E-4 0.0 0.0 3.914949547844185 2.9059790810189872E-5 58-59 3.7777728053246833E-4 0.0 0.0 4.343639581876106 2.9059790810189872E-5 60-61 3.7777728053246833E-4 0.0 0.0 4.7658274877142475 2.9059790810189872E-5 62-63 3.7777728053246833E-4 0.0 0.0 5.1976777739967766 2.9059790810189872E-5 64-65 3.7777728053246833E-4 0.0 0.0 5.650254956074674 2.9059790810189872E-5 66-67 3.7777728053246833E-4 0.0 0.0 6.121459464061902 2.9059790810189872E-5 68-69 3.7777728053246833E-4 0.0 0.0 6.633892550261088 2.9059790810189872E-5 70-71 3.923071759375633E-4 0.0 0.0 7.243327218184687 2.9059790810189872E-5 72-73 3.923071759375633E-4 0.0 0.0 7.793014956202536 2.9059790810189872E-5 74-75 3.923071759375633E-4 0.0 0.0 8.323966394095516 2.9059790810189872E-5 76-77 3.923071759375633E-4 0.0 0.0 8.882698992003036 2.9059790810189872E-5 78-79 3.923071759375633E-4 0.0 0.0 9.445507225571685 2.9059790810189872E-5 80-81 3.923071759375633E-4 0.0 0.0 10.062126926773105 2.9059790810189872E-5 82-83 3.923071759375633E-4 0.0 0.0 10.749739696923818 2.9059790810189872E-5 84-85 3.923071759375633E-4 0.0 0.0 11.39582602600677 2.9059790810189872E-5 86-87 3.995721236401108E-4 0.0 0.0 12.033274332271393 2.9059790810189872E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 705 0.0 59.366703 2 TCACGCA 935 0.0 43.76553 4 ATCACGC 1410 0.0 29.021822 3 TAACGCA 465 0.0 26.00049 4 GTACTAG 3445 0.0 24.714588 1 AGTACTT 61410 0.0 22.547699 12-13 GTATCAA 103400 0.0 21.935402 1 CTAACGC 1810 0.0 21.580517 3 TCAACGA 820 0.0 21.549185 3 GTGATCG 2765 0.0 20.181211 8 TCTAACG 1965 0.0 20.116198 2 GACGTTA 1165 0.0 19.957024 7 TACTTTT 71175 0.0 19.715357 14-15 GTATAAG 3550 0.0 19.658684 1 CTATTGA 2465 0.0 19.619175 9 GTTAATA 3755 0.0 19.576662 1 TAGTACT 4730 0.0 19.563793 4 GAGTACT 59680 0.0 19.297411 12-13 TACGGGC 3070 0.0 19.236517 4 ATACGGG 3170 0.0 19.06976 3 >>END_MODULE