FastQCFastQC Report
Fri 27 May 2016
ERR765437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765437_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13377284
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT242590.18134473335544046No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA236110.17650070074015026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195950.14647965909970964No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA160940.12030842733098887No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA592950.021.9754051
GTACATA35900.021.8588391
GTACTAG71050.021.0975321
GTATAAG64700.021.061961
AATACGG26850.020.125155
GTGATCG51150.020.1157868
CTATTGA82000.019.8817279
TAGGCAT95650.019.5516625
TGATCGC52950.019.4319639
GGGCTAT92350.019.179756
CATGGGG344250.018.8225084
CAACGCA642650.018.7103275
AGGGCTA90400.018.5560445
GGTCGCC76150.018.38658594
GTACAAG50550.018.3126051
TAGTACT79700.018.2763984
TATCAAC707100.018.2017462
ATCAACG661750.018.1424333
AACGCAG678650.018.1035756
GGCTATT96350.018.0909237
Produced by FastQC (version 0.11.5)