##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765437_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13377284
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.677279035116545	33.0	33.0	33.0	27.0	33.0
2	31.592979337210753	33.0	33.0	33.0	27.0	33.0
3	31.614823905958787	33.0	33.0	33.0	27.0	33.0
4	35.335888211687816	37.0	37.0	37.0	33.0	37.0
5	35.399857101037846	37.0	37.0	37.0	33.0	37.0
6	35.40147618903807	37.0	37.0	37.0	33.0	37.0
7	35.32461282873265	37.0	37.0	37.0	33.0	37.0
8	35.309301125699356	37.0	37.0	37.0	33.0	37.0
9	35.28071370840299	37.0	37.0	37.0	33.0	37.0
10-11	35.258268158170225	37.0	37.0	37.0	33.0	37.0
12-13	35.21288260008534	37.0	37.0	37.0	33.0	37.0
14-15	36.706565398477	37.0	37.0	40.0	33.0	40.0
16-17	36.74991597696513	37.0	37.0	40.0	33.0	40.0
18-19	36.73038884425269	38.5	37.0	40.0	33.0	40.0
20-21	36.826340309437995	40.0	37.0	40.0	33.0	40.0
22-23	36.81537253750462	40.0	37.0	40.0	33.0	40.0
24-25	36.80023807523261	37.0	37.0	40.0	33.0	40.0
26-27	36.759642615048016	37.0	37.0	40.0	33.0	40.0
28-29	36.7182406010069	37.0	37.0	40.0	33.0	40.0
30-31	36.63285398590626	37.0	37.0	40.0	33.0	40.0
32-33	36.5206978113046	37.0	37.0	40.0	33.0	40.0
34-35	36.40747034300834	37.0	37.0	40.0	33.0	40.0
36-37	36.31951205491339	37.0	37.0	40.0	33.0	40.0
38-39	36.21273537289034	37.0	37.0	40.0	33.0	40.0
40-41	36.106713589993305	37.0	37.0	40.0	27.0	40.0
42-43	35.96058781438744	37.0	37.0	40.0	27.0	40.0
44-45	35.79349717027762	37.0	37.0	40.0	27.0	40.0
46-47	35.60386801984618	37.0	37.0	40.0	27.0	40.0
48-49	35.33872866121404	37.0	33.0	40.0	27.0	40.0
50-51	35.228675716236566	37.0	33.0	40.0	27.0	40.0
52-53	35.066412397314735	37.0	33.0	40.0	27.0	40.0
54-55	34.909202944334595	37.0	33.0	40.0	27.0	40.0
56-57	34.740981577426325	37.0	33.0	40.0	27.0	40.0
58-59	34.55517868948585	37.0	33.0	40.0	27.0	40.0
60-61	34.369313531805105	37.0	33.0	38.5	27.0	40.0
62-63	34.14816404436058	37.0	33.0	37.0	27.0	40.0
64-65	33.944184783697494	37.0	33.0	37.0	27.0	40.0
66-67	33.754490934034145	37.0	33.0	37.0	27.0	40.0
68-69	33.55720604421645	37.0	33.0	37.0	27.0	40.0
70-71	33.37158305078968	37.0	33.0	37.0	27.0	40.0
72-73	33.15554192465376	37.0	33.0	37.0	24.5	37.0
74-75	32.93247556828426	37.0	33.0	37.0	22.0	37.0
76-77	32.74050270593044	37.0	33.0	37.0	22.0	37.0
78-79	32.57181098943552	37.0	33.0	37.0	22.0	37.0
80-81	32.41774440162891	35.0	33.0	37.0	22.0	37.0
82-83	32.27846717614727	33.0	33.0	37.0	22.0	37.0
84-85	32.13868491541332	33.0	33.0	37.0	22.0	37.0
86-87	32.02201960427841	33.0	33.0	37.0	22.0	37.0
88-89	31.910943207903784	33.0	33.0	37.0	22.0	37.0
90-91	31.783126343135123	33.0	33.0	37.0	22.0	37.0
92-93	31.682722815782334	33.0	33.0	37.0	22.0	37.0
94-95	31.57592367030557	33.0	33.0	37.0	22.0	37.0
96-97	31.495034156410227	33.0	33.0	37.0	22.0	37.0
98-99	31.392213359602742	33.0	33.0	37.0	22.0	37.0
100	31.33447170591579	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	3.0
6	6.0
7	149.0
8	866.0
9	1870.0
10	3032.0
11	6230.0
12	13316.0
13	25926.0
14	36522.0
15	40792.0
16	46637.0
17	55128.0
18	64922.0
19	76735.0
20	89376.0
21	105709.0
22	122484.0
23	124052.0
24	121479.0
25	128854.0
26	143160.0
27	167855.0
28	203797.0
29	254480.0
30	323123.0
31	415709.0
32	542199.0
33	708731.0
34	932253.0
35	1279264.0
36	2088379.0
37	3515984.0
38	1736052.0
39	2209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.67503249570369	20.50126936050474	13.413329275965442	24.410368867826133
2	15.664626740267588	21.451881562413025	38.418952753055876	24.464538944263516
3	17.615447004164565	27.27287475070869	29.92952000083131	25.182158244295426
4	12.650492436430008	17.980934385267812	37.92014683385025	31.448426344451924
5	13.420198879531082	38.22261281852401	33.91736554810486	14.439822753840042
6	29.888383920084227	38.67948082734881	17.847935350703477	13.584199901863487
7	25.911799435520695	32.89417343610258	23.128020605677506	18.066006522699226
8	26.08229742300455	33.64856423770326	20.489727212190456	19.779411127101735
9	26.609145772789155	14.949192975195864	20.652077058392422	37.78958419362256
10-11	24.810966859939583	26.20067720772019	27.482865729695206	21.50549020264502
12-13	25.62392336142374	24.36025130362785	27.275884252737704	22.73994108221071
14-15	23.581483356412257	24.830066402118696	25.28609693866109	26.30235330280795
16-17	22.79667299121422	27.27080541978703	26.113242405020497	23.81927918397825
18-19	22.98589972918985	26.821126593102303	28.11869642586891	22.07427725183894
20-21	24.592022551787704	25.917519422958897	26.845896864891856	22.64456116036154
22-23	24.17458854832394	25.596251142474856	26.6566875951564	23.572472714044803
24-25	23.487102435729167	26.247213971441447	27.020758699864427	23.244924892964956
26-27	23.588287809165855	26.221756747886865	26.808316459286463	23.381638983660817
28-29	23.059415493332647	26.234341007881913	27.270680965400828	23.435562533384616
30-31	23.761243903712824	25.912278064529083	27.074249831014097	23.252228200744
32-33	23.352526868425876	26.26898349774655	26.51005684045143	23.868432793376147
34-35	23.6581869029007	26.165867054658204	27.154604003371972	23.021342039069122
36-37	23.74067393281187	25.987875486512813	26.78425039364745	23.487200187027867
38-39	23.79148102975331	25.676955788273446	27.00459547742216	23.526967704551083
40-41	23.48800191904091	25.72578502599237	27.369648002104483	23.416565052862236
42-43	23.87212927409118	25.763580802007922	26.81642999052149	23.547859933379403
44-45	23.488048939857357	26.198202250366453	26.671648806191172	23.642100003585018
46-47	23.318967138263364	25.962673263692025	26.739231353734755	23.97912824430985
48-49	24.024482986674066	25.917926961489652	26.429441457989018	23.62814859384726
50-51	23.35400482738154	26.25764069698475	26.05029527350446	24.338059202129248
52-53	23.131326843566082	26.329378574944794	25.920857251353503	24.61843733013562
54-55	23.343541117846055	26.242719658565928	25.977681900630134	24.436057322957886
56-57	23.65800324980932	25.704916596351822	25.79437831135817	24.842701842480693
58-59	23.135805613892195	25.72045213996701	26.504851948134288	24.63889029800651
60-61	23.30945378036598	25.93821955386903	26.098308782754387	24.6540178830106
62-63	23.145956354948254	25.738150250876707	25.91244334642594	25.203450047749097
64-65	22.701456710345486	25.701634455959177	26.267246791467386	25.329662042227945
66-67	23.437915528528926	25.520252465648397	26.019479211097185	25.022352794725496
68-69	23.191927545301567	25.273242539437092	26.163122202001553	25.371707713259784
70-71	22.783845012260272	25.321724244734785	26.305970135305962	25.58846060769898
72-73	23.416075212686067	25.253374810807617	26.376058944664425	24.954491031841886
74-75	23.306153788534207	25.477034987836074	26.353796024758896	24.863015198870826
76-77	22.93225099534802	25.49226894942167	26.41624661253855	25.159233442691754
78-79	22.974479794222095	25.56473517196035	26.34186336806013	25.118921665757426
80-81	22.660900594498642	25.505562314261226	26.691147019216437	25.142390072023694
82-83	23.039305262526028	25.185182804139668	26.67660617505632	25.09890575827798
84-85	23.16170033925511	25.408901009901058	26.030907335107827	25.398491315736006
86-87	22.606101483573383	25.34228078546056	26.460921615976083	25.590696114989974
88-89	22.98166382892724	24.744723993102113	26.473109327529205	25.800502850441447
90-91	23.011222208687563	24.861619745776753	26.409617961693645	25.71754008384204
92-93	22.960267402459248	24.958908442271984	26.360523488597842	25.720300666670926
94-95	22.214654843165373	25.010295763707198	26.777578835572857	25.99747055755457
96-97	22.683445480144137	24.989295145109093	26.281278434770805	26.045980939975966
98-99	22.477547555137527	25.159054114393374	26.257177310604558	26.106221019864545
100	22.655243374521454	25.00512820666165	26.060940737367343	26.278687681449558
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	559.0
1	526.0
2	671.5
3	841.0
4	704.5
5	612.5
6	825.5
7	1580.5
8	2531.0
9	2509.0
10	1731.5
11	1364.5
12	1522.5
13	1825.5
14	2203.5
15	2793.5
16	3479.5
17	4210.5
18	5222.0
19	6416.0
20	7921.0
21	10024.0
22	12792.0
23	16219.5
24	20247.0
25	26076.5
26	35382.5
27	46006.0
28	58085.5
29	78956.5
30	103023.0
31	124155.5
32	152353.0
33	181558.0
34	211606.0
35	236294.0
36	258805.0
37	297029.5
38	317425.5
39	319696.0
40	321989.0
41	325512.0
42	337479.0
43	365112.5
44	407593.5
45	456085.0
46	508177.0
47	559151.0
48	606387.5
49	628249.5
50	639470.0
51	626259.0
52	580474.5
53	557162.5
54	534195.5
55	489679.0
56	453880.0
57	405146.0
58	358635.0
59	326541.0
60	276089.5
61	220774.0
62	177567.5
63	139711.5
64	104684.0
65	80428.0
66	62707.0
67	46979.0
68	36783.0
69	30807.5
70	26827.0
71	23478.5
72	20558.0
73	20781.0
74	16910.5
75	12786.5
76	10192.5
77	7292.5
78	5391.5
79	3629.5
80	2568.0
81	2010.0
82	1683.5
83	1374.0
84	933.5
85	605.0
86	411.5
87	219.0
88	111.5
89	77.5
90	61.0
91	40.0
92	25.0
93	20.0
94	17.5
95	15.5
96	9.0
97	6.0
98	9.0
99	7.0
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.018232400538106243
2	0.00613726971782912
3	0.006466185512694505
4	0.003976890974281476
5	0.04690787756318846
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	3.1770275640406525E-4
18-19	0.0014988094743297667
20-21	5.531765640917842E-4
22-23	0.004649673281960673
24-25	0.007030575115247609
26-27	0.005427110615278856
28-29	0.02137952666625004
30-31	0.019791012884229714
32-33	0.012607192910010731
34-35	0.012966010140772971
36-37	0.012984698538208505
38-39	0.0203441894483215
40-41	0.029430488281477766
42-43	0.017129785089409776
44-45	0.01624769273045261
46-47	0.017694174691962883
48-49	0.012782863845904746
50-51	0.009684327551093332
52-53	0.026638441704609096
54-55	0.02497143665335953
56-57	0.01293237102538901
58-59	0.015619762576618693
60-61	0.016767230179160434
62-63	0.02008255188422403
64-65	0.010663599576715274
66-67	0.014943242589452387
68-69	0.007333327153703248
70-71	0.008634039615216362
72-73	0.010084259256213743
74-75	0.003251781153782786
76-77	0.006970772243453903
78-79	0.005430848294765963
80-81	0.0022276569743155637
82-83	2.2052308973929236E-4
84-85	0.0024668684614903894
86-87	0.002179067140983177
88-89	0.0010876647307480353
90-91	7.8117501280529E-4
92-93	0.0010801893717738219
94-95	0.003162076846092226
96-97	0.001315663179461541
98-99	0.0017417586409916989
100	0.0021529033845734307
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.3377284E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.056104713766906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.03259262068681	24.531495170942236
2	14.790266075510154	10.07397700424096
3	5.425925627526097	5.543576740204205
4	2.5277182414444694	3.4433694847012633
5	1.4063383884385674	2.3947203709827027
6	0.8229866820166692	1.6816632372477165
7	0.5328515819912063	1.2702794491232212
8	0.37500437261540664	1.02169505455286
9	0.2730155438836765	0.8368061355889659
>10	1.4703155142007847	9.268134210737045
>50	0.14914702077712977	3.547489235250037
>100	0.14062417387090714	10.321795101124422
>500	0.02718295935384331	6.446875365946184
>1k	0.024592399941284312	15.668773496347722
>5k	0.0011953088940146839	2.7288994563222144
>10k+	2.4348884878076896E-4	1.2204504866883172
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	24259	0.18134473335544046	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	23611	0.17650070074015026	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19595	0.14647965909970964	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	16094	0.12030842733098887	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4950717948426602E-5
2	0.0	2.24260769226399E-5	0.0	0.0	1.4950717948426602E-5
3	0.0	2.24260769226399E-5	0.0	0.0	1.4950717948426602E-5
4	0.0	3.7376794871066506E-5	0.0	0.0	1.4950717948426602E-5
5	0.0	3.7376794871066506E-5	0.0	0.0	1.4950717948426602E-5
6	7.475358974213301E-6	3.7376794871066506E-5	0.0	0.0	1.4950717948426602E-5
7	7.475358974213301E-6	3.7376794871066506E-5	0.0	0.0	1.4950717948426602E-5
8	7.475358974213301E-6	3.7376794871066506E-5	0.0	0.0	1.4950717948426602E-5
9	7.475358974213301E-6	6.727823076791971E-5	0.0	0.0	1.4950717948426602E-5
10-11	7.475358974213301E-6	6.727823076791971E-5	0.0	0.0	1.4950717948426602E-5
12-13	1.4950717948426602E-5	6.727823076791971E-5	0.0	7.475358974213301E-6	8.222894871634631E-5
14-15	2.6163756409746552E-5	8.97043076905596E-5	0.0	7.475358974213301E-6	9.71796666647729E-5
16-17	2.9901435896853203E-5	8.97043076905596E-5	0.0	1.4950717948426602E-5	9.71796666647729E-5
18-19	2.9901435896853203E-5	9.71796666647729E-5	0.0	4.48521538452798E-5	1.0465502563898621E-4
20-21	4.48521538452798E-5	1.0465502563898621E-4	0.0	1.0839270512609287E-4	1.1213038461319951E-4
22-23	4.48521538452798E-5	1.2334342307451947E-4	0.0	4.634722564012246E-4	1.1960574358741281E-4
24-25	9.717966666477292E-5	1.3829414102294605E-4	0.0	0.0018763151025275385	1.1960574358741281E-4
26-27	1.420318205100527E-4	1.420318205100527E-4	0.0	0.003580696948648171	1.2334342307451947E-4
28-29	1.569825384584793E-4	1.4576949999715936E-4	0.0	0.014726457179200202	1.270811025616261E-4
30-31	1.569825384584793E-4	1.6445789743269263E-4	0.0	0.05833396375527349	1.3081878204873276E-4
32-33	1.6072021794558597E-4	1.6445789743269263E-4	0.0	0.14065261677931035	1.6445789743269263E-4
34-35	1.7193325640690592E-4	1.9809701281665247E-4	0.0	0.24604022759776947	1.7193325640690592E-4
36-37	1.7567093589401257E-4	2.317361282006123E-4	0.0	0.4154580257098526	1.7193325640690592E-4
38-39	1.794086153811192E-4	2.317361282006123E-4	0.0	0.6909063155121773	1.794086153811192E-4
40-41	1.8314629486822586E-4	2.616375640974655E-4	0.0	1.0178897300827283	1.794086153811192E-4
42-43	1.9435933332954584E-4	2.653752435845722E-4	0.0	1.3078813307693848	1.794086153811192E-4
44-45	2.0931005127797241E-4	2.9527667948142536E-4	0.0	1.6401759878911144	1.9809701281665247E-4
46-47	2.0931005127797241E-4	3.2144043589117194E-4	0.0	2.0094736719351998	2.2052308973929236E-4
48-49	2.0931005127797241E-4	3.2144043589117194E-4	0.0	2.4003826187737363	2.317361282006123E-4
50-51	2.0931005127797241E-4	3.476041923009185E-4	0.0	2.806391790740183	2.3921148717482562E-4
52-53	2.0931005127797241E-4	3.5134187178802515E-4	0.0	3.197951093809476	2.7658828204589215E-4
54-55	2.0931005127797241E-4	3.5134187178802515E-4	0.0	3.586060518712169	2.7658828204589215E-4
56-57	2.0931005127797241E-4	3.5134187178802515E-4	0.0	4.031203194908623	2.7658828204589215E-4
58-59	2.0931005127797241E-4	3.5134187178802515E-4	0.0	4.52664382396307	2.7658828204589215E-4
60-61	2.1678541025218573E-4	3.550795512751318E-4	0.0	5.032964838004485	2.7658828204589215E-4
62-63	2.2052308973929236E-4	3.7376794871066504E-4	0.0	5.546458458981659	3.064897179427453E-4
64-65	2.2426076922639902E-4	3.8124330768487836E-4	0.0	6.06582023675359	3.10227397429852E-4
66-67	2.2426076922639902E-4	3.84980987171985E-4	0.0	6.616279507858247	3.139650769169586E-4
68-69	2.2426076922639902E-4	3.924563461461983E-4	0.0	7.230929686474474	3.139650769169586E-4
70-71	2.2426076922639902E-4	4.036693846075182E-4	0.0	7.914794213832943	3.139650769169586E-4
72-73	2.2426076922639902E-4	4.148824230688382E-4	0.0	8.57623266426877	3.2517811537827857E-4
74-75	2.317361282006123E-4	4.4852153845279804E-4	0.0	9.220537591935702	3.4012883332670515E-4
76-77	2.317361282006123E-4	4.6720993588833125E-4	7.475358974213301E-6	9.921345020409225	3.5134187178802515E-4
78-79	2.317361282006123E-4	4.7094761537543793E-4	7.475358974213301E-6	10.644481346138722	3.5134187178802515E-4
80-81	2.317361282006123E-4	4.7094761537543793E-4	7.475358974213301E-6	11.433808237905392	3.5134187178802515E-4
82-83	2.317361282006123E-4	4.7094761537543793E-4	7.475358974213301E-6	12.266346442222503	3.662925897364517E-4
84-85	2.3921148717482562E-4	4.7094761537543793E-4	7.475358974213301E-6	13.056809588553254	3.700302692235584E-4
86-87	2.3921148717482562E-4	4.7842297434965125E-4	7.475358974213301E-6	13.869425961204083	3.812433076848783E-4
88	2.3921148717482562E-4	5.008490512722911E-4	7.475358974213301E-6	14.495580717281625	3.887186666590916E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	59295	0.0	21.975405	1
GTACATA	3590	0.0	21.858839	1
GTACTAG	7105	0.0	21.097532	1
GTATAAG	6470	0.0	21.06196	1
AATACGG	2685	0.0	20.12515	5
GTGATCG	5115	0.0	20.115786	8
CTATTGA	8200	0.0	19.881727	9
TAGGCAT	9565	0.0	19.551662	5
TGATCGC	5295	0.0	19.431963	9
GGGCTAT	9235	0.0	19.17975	6
CATGGGG	34425	0.0	18.822508	4
CAACGCA	64265	0.0	18.710327	5
AGGGCTA	9040	0.0	18.556044	5
GGTCGCC	7615	0.0	18.386585	94
GTACAAG	5055	0.0	18.312605	1
TAGTACT	7970	0.0	18.276398	4
TATCAAC	70710	0.0	18.201746	2
ATCAACG	66175	0.0	18.142433	3
AACGCAG	67865	0.0	18.103575	6
GGCTATT	9635	0.0	18.090923	7
>>END_MODULE