FastQCFastQC Report
Fri 27 May 2016
ERR765436_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765436_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6550779
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA181910.2776921645502008No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT167590.25583216896799604No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG69480.10606372158181493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG20650.023.6443391
GTACATA17000.021.335541
TAGGCAT32450.019.6313675
CTAGGCA35500.017.9447294
GTATAAG25350.017.6096691
GTACAAA29400.017.3981591
CATGGGG165800.017.3319994
GTATATA16700.016.9852261
TACATGA23950.016.5028172
GTACAAG28850.016.4403971
GTACATG531150.016.353761
ATGGGTA33500.016.1011875
TACATGG523750.015.9273342
TACTAGG30200.015.7049992
GTATCAA379600.015.6798431
CGTTAAC5950.015.6304311
CATGGGT48700.015.4678784
TAGTACT26150.015.2922524
ACATGGG490700.015.2470093
GTCCTAT35450.015.0847991
Produced by FastQC (version 0.11.5)