##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765436_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6550779 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.216001791542656 33.0 33.0 33.0 27.0 33.0 2 31.032494150695666 33.0 33.0 33.0 27.0 33.0 3 31.148618202506906 33.0 33.0 33.0 27.0 33.0 4 34.72303095555505 37.0 37.0 37.0 27.0 37.0 5 34.835872802303356 37.0 37.0 37.0 33.0 37.0 6 34.763954180105905 37.0 37.0 37.0 33.0 37.0 7 34.67027677166334 37.0 37.0 37.0 27.0 37.0 8 34.58280915903284 37.0 37.0 37.0 27.0 37.0 9 34.53499301380798 37.0 37.0 37.0 27.0 37.0 10-11 34.519629115865456 37.0 37.0 37.0 27.0 37.0 12-13 34.454037909079204 37.0 37.0 37.0 27.0 37.0 14-15 35.81032515369546 37.0 37.0 40.0 27.0 40.0 16-17 35.86355470089893 37.0 37.0 40.0 27.0 40.0 18-19 35.868560823071576 37.0 37.0 40.0 27.0 40.0 20-21 35.881802606987655 37.0 37.0 40.0 27.0 40.0 22-23 35.92973744038686 37.0 37.0 40.0 27.0 40.0 24-25 35.8887750601875 37.0 37.0 40.0 27.0 40.0 26-27 35.895960465159945 37.0 37.0 40.0 27.0 40.0 28-29 35.822082228693716 37.0 37.0 40.0 27.0 40.0 30-31 35.78593958062087 37.0 37.0 40.0 27.0 40.0 32-33 35.64774372635682 37.0 33.0 40.0 27.0 40.0 34-35 35.5920006612954 37.0 33.0 40.0 27.0 40.0 36-37 35.47370030342956 37.0 33.0 40.0 27.0 40.0 38-39 35.253162562803595 37.0 33.0 40.0 27.0 40.0 40-41 35.16615916977202 37.0 33.0 40.0 27.0 40.0 42-43 35.02901830454057 37.0 33.0 40.0 27.0 40.0 44-45 34.87692891181339 37.0 33.0 40.0 27.0 40.0 46-47 34.739055614607054 37.0 33.0 40.0 27.0 40.0 48-49 34.56544038502902 37.0 33.0 40.0 27.0 40.0 50-51 34.39204696113241 37.0 33.0 40.0 27.0 40.0 52-53 34.19532470871022 37.0 33.0 40.0 22.0 40.0 54-55 34.0552784638285 37.0 33.0 38.5 22.0 40.0 56-57 33.84907146157732 37.0 33.0 37.0 22.0 40.0 58-59 33.614641861677825 37.0 33.0 37.0 22.0 40.0 60-61 33.371445518158986 37.0 33.0 37.0 22.0 40.0 62-63 33.18964996376767 37.0 33.0 37.0 22.0 40.0 64-65 32.88185603574781 37.0 33.0 37.0 22.0 40.0 66-67 32.65896727702156 37.0 33.0 37.0 22.0 40.0 68-69 32.360341418936585 35.0 33.0 37.0 22.0 37.0 70-71 32.22155471891206 33.0 33.0 37.0 22.0 37.0 72-73 31.94281741148648 33.0 33.0 37.0 22.0 37.0 74-75 31.660178201707005 33.0 30.0 37.0 22.0 37.0 76-77 31.474453877928106 33.0 27.0 37.0 22.0 37.0 78-79 31.240489337222336 33.0 27.0 37.0 22.0 37.0 80-81 30.967132534924474 33.0 27.0 37.0 22.0 37.0 82-83 30.786791921998898 33.0 27.0 37.0 18.5 37.0 84-85 30.592606467108723 33.0 27.0 37.0 15.0 37.0 86-87 30.36942980369205 33.0 27.0 37.0 15.0 37.0 88-89 30.181945536553744 33.0 27.0 37.0 15.0 37.0 90-91 29.967556530299678 33.0 27.0 37.0 15.0 37.0 92-93 29.81318099725239 33.0 27.0 37.0 15.0 37.0 94-95 29.5668054135241 33.0 27.0 37.0 15.0 37.0 96-97 29.478698792922188 33.0 27.0 37.0 15.0 37.0 98-99 28.320602481017907 33.0 24.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3588022440725851 1101 2 0.28670775061744536 1101 3 0.3503048148060053 1101 4 0.45274612872884745 1101 5 0.4673012493056632 1101 6 0.38117948096922305 1101 7 0.3084037255202432 1101 8 0.37286882353376427 1101 9 0.2548618006668306 1101 10-11 0.3237173972754306 1101 12-13 0.33329717289064575 1101 14-15 0.48684287133176696 1101 16-17 0.47110354312125935 1101 18-19 0.4156883625891865 1101 20-21 0.4163743720133084 1101 22-23 0.34565792522423067 1101 24-25 0.3909660482155104 1101 26-27 0.4205168386649163 1101 28-29 0.297499777021649 1101 30-31 0.4267942043086137 1101 32-33 0.533155525666352 1101 34-35 0.509938299052692 1101 36-37 0.5351187934194144 1101 38-39 0.4427038234985332 1101 40-41 0.43644088442493967 1101 42-43 0.4265360486289538 1101 44-45 0.4428484027481048 1101 46-47 0.39267745432983503 1101 48-49 0.394947516254085 1101 50-51 0.40341933375691497 1101 52-53 0.43344701125238316 1101 54-55 0.5147767291637351 1101 56-57 0.5238576969035478 1101 58-59 0.34430344806746405 1101 60-61 0.4949726284966687 1101 62-63 0.49589758699502795 1101 64-65 0.3383405280885299 1101 66-67 0.3688807470235247 1101 68-69 0.3804423458026349 1101 70-71 0.3618068542915509 1101 72-73 0.44997877984404866 1101 74-75 0.4361740882327503 1101 76-77 0.3305468202661572 1101 78-79 0.4062707259375138 1101 80-81 0.34520804321759613 1101 82-83 0.42525788901060935 1101 84-85 0.32858761030990635 1101 86-87 0.4151681639448306 1101 88-89 0.5281373140082479 1101 90-91 0.4951846720760784 1101 92-93 0.5189389494897867 1101 94-95 0.5063667750298428 1101 96-97 0.48993810536475024 1101 98-99 0.5169869812981958 1102 1 0.32105440882450864 1102 2 0.3460689230990184 1102 3 0.3104522776043517 1102 4 0.457219100131681 1102 5 0.3482186148300528 1102 6 0.35872638054909345 1102 7 0.3076275827835744 1102 8 0.35918081632710397 1102 9 0.29190365779869154 1102 10-11 0.30315748113464736 1102 12-13 0.33226891495665 1102 14-15 0.43794833298533575 1102 16-17 0.4863501947593747 1102 18-19 0.4537995382090827 1102 20-21 0.4336605720081863 1102 22-23 0.38236730556824483 1102 24-25 0.49181068660337957 1102 26-27 0.5266320038004793 1102 28-29 0.3503155236787805 1102 30-31 0.3847710449831325 1102 32-33 0.46351541635483784 1102 34-35 0.4242294987115045 1102 36-37 0.5013652996452436 1102 38-39 0.4504788382506888 1102 40-41 0.38330656734226665 1102 42-43 0.41386985293677725 1102 44-45 0.33460366575317124 1102 46-47 0.36599823973072887 1102 48-49 0.34325613217313844 1102 50-51 0.30317415579190055 1102 52-53 0.3500004102636751 1102 54-55 0.42250294765843677 1102 56-57 0.3907553604412328 1102 58-59 0.39146838067300394 1102 60-61 0.4688975663671755 1102 62-63 0.37680504880228227 1102 64-65 0.2965743303507864 1102 66-67 0.40341884671082795 1102 68-69 0.3431673268729014 1102 70-71 0.34939471852660375 1102 72-73 0.33299731285258005 1102 74-75 0.3193965400634333 1102 76-77 0.31288782892368516 1102 78-79 0.27867337450736684 1102 80-81 0.26807650502020053 1102 82-83 0.24908591759406562 1102 84-85 0.1839919509388359 1102 86-87 0.3135156188984034 1102 88-89 0.340011278182331 1102 90-91 0.3068452355834843 1102 92-93 0.39778101552474965 1102 94-95 0.4564025066123385 1102 96-97 0.24265589114013508 1102 98-99 0.32921881178952717 1103 1 0.04680643844904964 1103 2 0.10323699561631372 1103 3 0.13645363413756684 1103 4 0.17523438487580023 1103 5 0.09381791878482204 1103 6 0.0709345097631413 1103 7 -0.05581007209440969 1103 8 0.021041105582852992 1103 9 0.11093189780034862 1103 10-11 0.043289889249010116 1103 12-13 -0.05003155349786681 1103 14-15 0.004511646189861551 1103 16-17 -0.03380473497244907 1103 18-19 0.055865785495100795 1103 20-21 0.09021077652322163 1103 22-23 0.04480972292505214 1103 24-25 0.1346751879312933 1103 26-27 0.256833194481068 1103 28-29 0.15035461229303593 1103 30-31 0.233298372308262 1103 32-33 0.2601687734092337 1103 34-35 0.2165604891634061 1103 36-37 0.2662259749162743 1103 38-39 0.14289817384167236 1103 40-41 0.08488627706363872 1103 42-43 0.1663142612557067 1103 44-45 0.141619767583272 1103 46-47 0.1468488372020218 1103 48-49 0.1391289346562985 1103 50-51 0.19847353682795443 1103 52-53 0.20560901755217742 1103 54-55 0.18636077469680146 1103 56-57 0.09322622359333366 1103 58-59 0.14030744274109708 1103 60-61 0.21451373565862752 1103 62-63 0.1795820147708298 1103 64-65 0.1505444123394568 1103 66-67 0.23530647973084484 1103 68-69 0.24868210931005308 1103 70-71 0.16465550412251417 1103 72-73 0.19138611305131192 1103 74-75 0.1595911733550004 1103 76-77 0.15023005887432817 1103 78-79 0.12080657778886206 1103 80-81 0.0909452927008303 1103 82-83 0.20566017195744024 1103 84-85 0.12098360107503936 1103 86-87 0.2789158631718003 1103 88-89 0.2614484301444939 1103 90-91 0.23497308579494458 1103 92-93 0.30908879756858454 1103 94-95 0.16459473735449492 1103 96-97 0.036728669025478666 1103 98-99 0.18481725116746972 1104 1 0.19358637958964664 1104 2 0.08962416817131214 1104 3 0.13320578039049025 1104 4 0.1907403592242929 1104 5 0.15911464002638098 1104 6 0.15413679842096428 1104 7 0.03629117153467831 1104 8 0.09137440705462296 1104 9 0.18284568549993452 1104 10-11 0.1572216478881998 1104 12-13 0.06177552239546458 1104 14-15 0.1387353422553801 1104 16-17 0.17654009366680157 1104 18-19 0.0740604482455467 1104 20-21 0.07255761274633699 1104 22-23 0.13836229638758368 1104 24-25 0.1698830703802443 1104 26-27 0.1466102286866473 1104 28-29 0.07752107898513572 1104 30-31 0.0794850318004805 1104 32-33 0.2245871690746739 1104 34-35 0.15068172434561689 1104 36-37 0.22835977943428531 1104 38-39 0.1134316992808877 1104 40-41 0.16018109068316733 1104 42-43 0.07763106098257566 1104 44-45 0.049591255861322736 1104 46-47 0.14416227120771197 1104 48-49 0.18433863514844262 1104 50-51 0.1850180780209314 1104 52-53 0.015833194696767805 1104 54-55 0.19476018514590265 1104 56-57 0.13765374306680656 1104 58-59 0.15828787465156324 1104 60-61 0.18526224521440326 1104 62-63 0.11729776361579525 1104 64-65 0.14071310964636297 1104 66-67 0.1468574721269249 1104 68-69 0.018028078959730465 1104 70-71 -0.009608745707161859 1104 72-73 0.05353499328023403 1104 74-75 -0.011917081792805817 1104 76-77 -0.03233200068154929 1104 78-79 -0.018546275180000293 1104 80-81 0.04380683771791283 1104 82-83 0.07367143731040926 1104 84-85 0.04709145460427422 1104 86-87 0.10564713959183436 1104 88-89 0.17309193304750892 1104 90-91 0.0428943590278017 1104 92-93 0.09719180182897347 1104 94-95 0.1216992755506574 1104 96-97 -0.08681820192979472 1104 98-99 0.10266354386081744 1105 1 0.06317382813194072 1105 2 0.11144839059312162 1105 3 0.07681205329611629 1105 4 0.13874976560826013 1105 5 0.13243989355585484 1105 6 0.11852520070949168 1105 7 0.0689322011248592 1105 8 0.03993792195079493 1105 9 -0.02610145034991973 1105 10-11 0.06346498022247715 1105 12-13 0.06809420461440396 1105 14-15 0.04680691506700896 1105 16-17 0.056724530986521415 1105 18-19 0.044885180612546094 1105 20-21 0.06668462212071091 1105 22-23 0.010968696086308682 1105 24-25 0.04805498114713913 1105 26-27 0.01193840727989226 1105 28-29 0.06046852690228377 1105 30-31 0.03310874064722924 1105 32-33 0.09460521503427799 1105 34-35 0.01524396677351092 1105 36-37 -0.019321033695128165 1105 38-39 0.02185247489668285 1105 40-41 -0.12495048551129173 1105 42-43 -0.04761692286857766 1105 44-45 -0.12358986694069074 1105 46-47 -0.0956478164307697 1105 48-49 -0.13647437831002662 1105 50-51 -0.06877749470700678 1105 52-53 -0.09590082817589973 1105 54-55 0.021587411026416703 1105 56-57 0.038471117401108756 1105 58-59 -0.02862870128956274 1105 60-61 0.0300750338230813 1105 62-63 0.0249198032301976 1105 64-65 -0.13491005320866378 1105 66-67 -0.09987186343440158 1105 68-69 -0.06180456423769698 1105 70-71 -0.1808047877460055 1105 72-73 -0.03279854112392755 1105 74-75 0.053384961794275654 1105 76-77 -0.08965343983107132 1105 78-79 -0.03134901007933166 1105 80-81 -0.05963266823770752 1105 82-83 -0.0010052390367292219 1105 84-85 -0.0290415374884212 1105 86-87 -0.003701887516935898 1105 88-89 -0.050878783041071074 1105 90-91 0.014829687995383978 1105 92-93 -0.07512980999106844 1105 94-95 -0.015172988346357386 1105 96-97 -0.07752846256648738 1105 98-99 0.01552542534431467 1106 1 0.0727952738283193 1106 2 0.10754487382860134 1106 3 0.08752696387273673 1106 4 -0.04814645651124749 1106 5 0.03150024936320506 1106 6 0.04322093625737011 1106 7 0.017934522974364597 1106 8 -0.0541092724524006 1106 9 -0.0352154194158274 1106 10-11 -0.027066947786920537 1106 12-13 -0.01028968105255501 1106 14-15 -0.02098491125648394 1106 16-17 -0.03949912594363525 1106 18-19 -0.03969888315948822 1106 20-21 -0.05722951905776341 1106 22-23 -0.05850596274622433 1106 24-25 0.039792345437810184 1106 26-27 0.10765917528306801 1106 28-29 -0.025508872518209103 1106 30-31 -0.07549809562038234 1106 32-33 0.04007969605645911 1106 34-35 -0.14385944664488193 1106 36-37 -0.1147909564851588 1106 38-39 -0.08097804973716904 1106 40-41 0.04412823093375806 1106 42-43 -0.04477068019281205 1106 44-45 -0.04298029180427676 1106 46-47 -0.08289415793371546 1106 48-49 -0.14804625126699023 1106 50-51 -0.06688926177348264 1106 52-53 -0.11196042313179078 1106 54-55 -0.15031086671826444 1106 56-57 -0.06686061432924362 1106 58-59 -0.01118847282781843 1106 60-61 0.0033301224163579946 1106 62-63 -0.033208860731328116 1106 64-65 -0.15879185942780083 1106 66-67 -0.0821670462966253 1106 68-69 -0.06194048600507074 1106 70-71 -0.06341290699878499 1106 72-73 -8.254700592225106E-4 1106 74-75 -0.11623600253706812 1106 76-77 -0.09550756559603713 1106 78-79 -0.012443049650485705 1106 80-81 -0.08593793618857504 1106 82-83 -0.08367392186972822 1106 84-85 -0.2273856712450275 1106 86-87 -0.22442315898863896 1106 88-89 -0.04534898433515622 1106 90-91 -0.07500432948209479 1106 92-93 -0.07124853916773333 1106 94-95 -0.13447734710673487 1106 96-97 -0.2729567178098087 1106 98-99 0.11986413539408858 1107 1 -0.018944865748274253 1107 2 0.02971481271847054 1107 3 0.031181062828206763 1107 4 -0.08206476371512395 1107 5 0.022679003962451816 1107 6 -0.09090782051104185 1107 7 -0.16981324633284345 1107 8 -0.15554585857735503 1107 9 -0.18787802367855022 1107 10-11 -0.0849951920567662 1107 12-13 -0.18710107534484877 1107 14-15 -0.13880275808123343 1107 16-17 -0.17214082639429762 1107 18-19 -0.2451700605981415 1107 20-21 -0.1993880006044222 1107 22-23 -0.16885589435858606 1107 24-25 -0.06031933674860568 1107 26-27 -0.0597671855178632 1107 28-29 -0.05593328311953627 1107 30-31 -0.06479571124483385 1107 32-33 -0.2638497571257048 1107 34-35 -0.22356510108377847 1107 36-37 -0.18199762016423904 1107 38-39 -0.13822613881441725 1107 40-41 -0.3033133719212344 1107 42-43 -0.21816884007429138 1107 44-45 -0.23804408724095083 1107 46-47 -0.34611662924572784 1107 48-49 -0.2820955926512383 1107 50-51 -0.19690779462931118 1107 52-53 -0.23681960367824217 1107 54-55 -0.17452498176226072 1107 56-57 -0.05810931050068291 1107 58-59 -0.09351833467018622 1107 60-61 -0.05855035743530124 1107 62-63 -0.27859531648834945 1107 64-65 -0.30214029123174413 1107 66-67 -0.1470344438345066 1107 68-69 -0.22692186678736448 1107 70-71 -0.1791910295908039 1107 72-73 -0.15486823734968524 1107 74-75 -0.19856748185951645 1107 76-77 -0.299488018503169 1107 78-79 -0.11852696898498039 1107 80-81 -0.3530325344924954 1107 82-83 -0.2483296740631502 1107 84-85 -0.3695851145122333 1107 86-87 -0.36085871194818253 1107 88-89 -0.26587019784651744 1107 90-91 -0.16836554515548485 1107 92-93 -0.14764841571548715 1107 94-95 -0.19206590611547725 1107 96-97 -0.22659788501193745 1107 98-99 0.06197557233971551 1108 1 0.03499754959153378 1108 2 0.029614453240242256 1108 3 0.004691109549565198 1108 4 0.04276457282697521 1108 5 0.04053394969277235 1108 6 -0.15644593842087318 1108 7 -0.05026600662678504 1108 8 -0.11221569905898576 1108 9 -0.05300244164116208 1108 10-11 -0.09366308648769461 1108 12-13 -0.07272833988104566 1108 14-15 -0.15672898331009577 1108 16-17 -0.07162587900873518 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0.1634923614143311 2215 96-97 0.1767941910748796 2215 98-99 0.13778870812068078 2216 1 -0.11741487779327286 2216 2 -0.03897621199445922 2216 3 -0.03677856407591662 2216 4 -0.06312137096294634 2216 5 -0.15618584533661561 2216 6 -0.026367767444469337 2216 7 0.01659467913964363 2216 8 -0.09012386605429157 2216 9 -0.043365630830244584 2216 10-11 -0.043097917925663864 2216 12-13 -0.048171892236261726 2216 14-15 -0.07063043922740775 2216 16-17 -0.08665188841548144 2216 18-19 -0.1570511971453925 2216 20-21 -0.1628564627960074 2216 22-23 -0.2981966343281215 2216 24-25 -0.15048128610002465 2216 26-27 -0.22919457199228788 2216 28-29 -0.16636677647527875 2216 30-31 -0.13827356818626413 2216 32-33 -0.11637791674410636 2216 34-35 -0.23530009844260746 2216 36-37 -0.27427755337143367 2216 38-39 -0.14718248951854207 2216 40-41 -0.21183739638630783 2216 42-43 -0.23380497960459223 2216 44-45 -0.13898816559412808 2216 46-47 -0.03611816080675112 2216 48-49 -0.00960910409092719 2216 50-51 0.001849836274530503 2216 52-53 0.14724741715799894 2216 54-55 0.028936332091788586 2216 56-57 0.113630454990016 2216 58-59 -0.10137122799353193 2216 60-61 -0.05916674998805149 2216 62-63 -0.004402037103005796 2216 64-65 0.06092612588502533 2216 66-67 -0.01036447333476076 2216 68-69 -0.05717935529558815 2216 70-71 -0.01128353120862613 2216 72-73 -0.02303101432483956 2216 74-75 0.021582841858542423 2216 76-77 -0.04861160844077972 2216 78-79 6.665804874614878E-4 2216 80-81 0.20071924297412025 2216 82-83 0.08663235279541226 2216 84-85 0.1038626867396637 2216 86-87 0.012382992269596826 2216 88-89 -0.006683952876958443 2216 90-91 0.12323735947449777 2216 92-93 0.297082072579272 2216 94-95 0.186483419856863 2216 96-97 0.15962068645810845 2216 98-99 0.2830577119212414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 175.0 8 1054.0 9 2099.0 10 3592.0 11 6903.0 12 13734.0 13 24293.0 14 33658.0 15 36869.0 16 38881.0 17 40282.0 18 42828.0 19 45625.0 20 49530.0 21 54301.0 22 61167.0 23 69510.0 24 79375.0 25 89061.0 26 100149.0 27 113154.0 28 132582.0 29 163639.0 30 202786.0 31 253348.0 32 325952.0 33 425266.0 34 570011.0 35 805180.0 36 1167492.0 37 1261164.0 38 336853.0 39 263.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.881894736552866 18.56350937795153 12.566087144887264 24.98850874060835 2 16.066536839828313 20.864959988715842 37.360835210845984 25.707667960609864 3 18.0675041048597 26.186913542403857 28.633602268557052 27.111980084179393 4 13.02490894594368 17.382054867062376 35.96546303882332 33.62757314817062 5 14.638655952215759 37.400361086826464 32.76018012514237 15.200802835815406 6 32.10873088528861 36.40058686150151 17.4206151665321 14.070067086677781 7 28.21925453446071 31.78144156595727 21.769762038988034 18.229541860593983 8 27.164967708420633 33.18586995531371 19.805003343877118 19.84415899238854 9 27.1704021765961 15.706070987893195 19.004869497200257 38.11865733831045 10-11 25.561708004498396 25.78362054344987 26.339684181072204 22.31498727097953 12-13 26.281889527947744 23.75116760922632 25.98838245039254 23.978560412433392 14-15 24.48143190298436 24.714129418806525 23.278185693640406 27.526252984568707 16-17 24.334113545885153 26.878215552684647 24.356927626470075 24.430743274960122 18-19 24.81231621460593 26.349507440260155 25.467284119949703 23.37089222518421 20-21 26.20698572272748 25.644249576499583 24.243702372899882 23.905062327873054 22-23 25.326537500349193 25.47108519460052 24.196977183934916 25.00540012111537 24-25 24.721183816673747 26.102229483746108 24.285385521656146 24.891201177924 26-27 24.9912796604128 25.965370214861533 24.138014467596744 24.90533565712892 28-29 24.503576554073412 25.838819845930587 24.879716046590428 24.777887553405574 30-31 25.009714541693835 25.85714467809127 24.54356156202961 24.589579218185285 32-33 24.477703099602248 26.06513335214026 24.13019465174813 25.32696889650936 34-35 24.87601658765439 26.080767078645877 24.694885361282797 24.348330972416928 36-37 24.91753270870533 26.078776279889766 24.26782371989652 24.735867291508384 38-39 25.03229196346663 25.709188610178163 24.322322911696677 24.936196514658526 40-41 24.711198469678187 26.083042948021905 24.66891342235786 24.536845159942047 42-43 25.32456826890359 25.845796354906796 24.41550844563677 24.41412693055284 44-45 24.788235109137403 26.128205515710423 24.292278826686108 24.79128054846607 46-47 24.325366494580265 26.072433522791716 24.940270462490034 24.66192952013799 48-49 24.985114341927975 26.173387137707582 24.52000860662379 24.321489913740653 50-51 24.223645767930808 26.426490650959224 24.435605292133957 24.91425828897601 52-53 24.301460940752236 26.032316156597556 24.300789264910325 25.365433637739876 54-55 24.656075254561326 25.867373941328196 24.425041662983897 25.051509141126576 56-57 24.750254893349325 25.420014932575196 24.20945661577043 25.62027355830505 58-59 24.50322320444637 25.43480706645729 24.888490361222686 25.173479367873654 60-61 24.50121580960066 25.71074371460249 24.57977898506422 25.20826149073263 62-63 24.062374871751892 25.747586661067334 24.467158791343746 25.722879675837024 64-65 24.09705776977059 25.402154461324372 24.690544437539412 25.81024333136563 66-67 24.71557576825226 25.308360319949784 24.56884510511576 25.407218806682195 68-69 24.731921195937154 24.962512092073325 24.569497765074964 25.736068946914557 70-71 24.010365790083895 25.078536461083484 24.829520275374882 26.081577473457735 72-73 24.67343960161074 25.122088533287418 24.79239491974924 25.412076945352606 74-75 24.91567033478003 24.867225714682178 24.80501173982514 25.41209221071265 76-77 24.370391674028387 25.130614236871672 25.08643628490596 25.412557804193973 78-79 24.514069242757238 25.09271798056384 24.993714488002112 25.399498288676813 80-81 24.08989068323019 24.89458123988002 25.438669202548276 25.576858874341507 82-83 24.367040927498852 25.17100637954662 24.872454100497055 25.589498592457478 84-85 24.40547147140821 25.208307286812758 24.590411308334474 25.79580993344456 86-87 24.220745349522552 24.838946635201705 24.9039923343468 26.03631568092894 88-89 24.368933832144236 24.377222922647825 25.178654324928377 26.075188920279558 90-91 24.686651770728336 24.642435655362515 24.946758240508498 25.724154333400655 92-93 24.318268102160065 24.82124645023134 24.903786251986215 25.956699195622384 94-95 23.74497750572871 24.926066044969613 25.351565058140412 25.977391391161262 96-97 24.497597919270365 24.732165441697852 24.856997923453072 25.913238715578714 98-99 24.28801215855397 24.708756012071238 24.80723284971146 26.195998979663337 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1151.0 1 1100.0 2 1046.5 3 1036.5 4 971.0 5 832.5 6 710.0 7 656.5 8 626.0 9 613.5 10 588.5 11 566.5 12 598.0 13 624.0 14 669.5 15 789.0 16 897.0 17 1098.5 18 1438.0 19 1910.5 20 2660.5 21 3616.0 22 4810.5 23 6382.5 24 8499.5 25 11642.5 26 15621.5 27 19639.0 28 25082.0 29 32547.0 30 39476.5 31 47246.5 32 56544.5 33 66235.5 34 75817.0 35 85046.5 36 91950.5 37 100771.5 38 111941.5 39 113991.5 40 116728.5 41 125856.5 42 139521.5 43 160446.0 44 181087.5 45 203452.0 46 237985.5 47 269027.5 48 289005.0 49 307851.5 50 329015.0 51 321724.5 52 301503.0 53 300610.5 54 291687.0 55 281037.5 56 259344.5 57 240424.5 58 230489.5 59 204631.0 60 171763.0 61 134536.5 62 109860.5 63 89661.5 64 66721.0 65 51214.5 66 37429.0 67 28087.5 68 22212.0 69 18953.5 70 16932.0 71 14838.5 72 14367.0 73 12915.0 74 9531.5 75 7115.5 76 5127.5 77 3540.0 78 2355.0 79 1552.5 80 1065.5 81 751.0 82 554.5 83 400.0 84 293.5 85 211.0 86 145.0 87 106.0 88 63.0 89 40.5 90 35.0 91 27.0 92 18.5 93 13.5 94 10.0 95 7.5 96 7.0 97 3.5 98 2.5 99 7.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002259273286428988 2 3.053072008687822E-5 3 1.0685752030407376E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.7477648078190394E-4 22-23 0.0 24-25 0.004144545251793718 26-27 3.053072008687822E-5 28-29 0.00838831534386979 30-31 4.961242014117711E-4 32-33 4.5796080130317326E-5 34-35 5.342876015203688E-5 36-37 0.0 38-39 7.632680021719555E-6 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 1.6028628045611064E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.526536004343911E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6550779.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.900795124824725 #Duplication Level Percentage of deduplicated Percentage of total 1 75.82483805969105 31.77121004912127 2 13.127533300247432 11.001081666159635 3 4.510621539865994 5.669958870826394 4 2.0234136333282553 3.391305604114578 5 1.1212715634238046 2.349108502915638 6 0.6894876683678013 1.7334048920023402 7 0.4689385490251833 1.375422864818575 8 0.3345379691580938 1.1213925525734576 9 0.24933121158207525 0.9402458413252376 >10 1.360603886107382 10.596838215274426 >50 0.1353798466607767 3.9761781360604256 >100 0.12451958680754664 10.956288985649747 >500 0.01794836041480659 5.227653490907021 >1k 0.011208533379571027 8.655047094884722 >5k 2.9303355239937315E-4 0.698453305128751 >10k+ 7.325838809984329E-5 0.5364099282378889 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 18191 0.2776921645502008 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 16759 0.25583216896799604 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 6948 0.10606372158181493 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.5796080130317326E-5 0.0 0.0 0.0 3 0.0 6.106144017375643E-5 0.0 0.0 0.0 4 0.0 7.632680021719554E-5 0.0 0.0 0.0 5 0.0 7.632680021719554E-5 0.0 1.5265360043439107E-5 0.0 6 0.0 7.632680021719554E-5 0.0 1.5265360043439107E-5 0.0 7 3.0530720086878215E-5 7.632680021719554E-5 0.0 1.5265360043439107E-5 0.0 8 4.5796080130317326E-5 7.632680021719554E-5 0.0 1.5265360043439107E-5 0.0 9 6.106144017375643E-5 1.2212288034751286E-4 0.0 3.0530720086878215E-5 0.0 10-11 1.0685752030407376E-4 1.2212288034751286E-4 0.0 3.0530720086878215E-5 0.0 12-13 1.2975556036923241E-4 1.2212288034751286E-4 0.0 3.816340010859777E-5 0.0 14-15 1.3738824039095197E-4 1.2212288034751286E-4 0.0 4.5796080130317326E-5 0.0 16-17 1.3738824039095197E-4 1.2212288034751286E-4 0.0 9.92248402823542E-5 0.0 18-19 1.6028628045611064E-4 1.4502092041267153E-4 0.0 1.2212288034751286E-4 0.0 20-21 1.831843205212693E-4 1.5265360043439108E-4 0.0 2.671438007601844E-4 7.632680021719554E-6 22-23 1.831843205212693E-4 1.5265360043439108E-4 0.0 0.0013586170438660808 2.289804006515866E-5 24-25 1.831843205212693E-4 1.984496805647084E-4 0.0 0.0036560537304036666 3.0530720086878215E-5 26-27 2.442457606950257E-4 1.984496805647084E-4 0.0 0.006228266897723156 3.0530720086878215E-5 28-29 2.442457606950257E-4 1.984496805647084E-4 0.0 0.017219326128999315 3.0530720086878215E-5 30-31 2.7477648078190394E-4 2.1371504060814752E-4 0.0 0.0503527901032839 3.0530720086878215E-5 32-33 2.976745208470626E-4 2.1371504060814752E-4 0.0 0.10539967841992533 3.0530720086878215E-5 34-35 3.2057256091222127E-4 2.442457606950257E-4 0.0 0.1735900417339678 3.0530720086878215E-5 36-37 3.358379209556604E-4 2.5951112073846483E-4 0.0 0.2804933581181719 3.0530720086878215E-5 38-39 3.358379209556604E-4 2.824091608036235E-4 0.0 0.438291384887202 3.0530720086878215E-5 40-41 3.511032809990995E-4 2.9004184082534305E-4 0.0 0.6317798234377927 3.0530720086878215E-5 42-43 3.511032809990995E-4 3.1293988089050174E-4 0.0 0.8049348024105225 3.0530720086878215E-5 44-45 3.58735961020819E-4 3.511032809990995E-4 0.0 1.0022090502518861 3.0530720086878215E-5 46-47 3.663686410425386E-4 3.663686410425386E-4 0.0 1.2167026242222492 3.0530720086878215E-5 48-49 3.663686410425386E-4 4.4269544125973416E-4 0.0 1.4408897018201958 4.5796080130317326E-5 50-51 3.740013210642582E-4 5.190222414769297E-4 0.0 1.683677620631073 4.5796080130317326E-5 52-53 3.816340010859777E-4 5.342876015203688E-4 0.0 1.9294728153705079 5.3428760152036874E-5 54-55 3.816340010859777E-4 5.342876015203688E-4 0.0 2.172749225702775 6.869412019547598E-5 56-57 3.968993611294168E-4 5.342876015203688E-4 0.0 2.4441673272751228 7.632680021719554E-5 58-59 4.1216472117285594E-4 5.571856415855275E-4 0.0 2.7367126871475898 8.39594802389151E-5 60-61 4.2743008121629505E-4 5.953490416941253E-4 0.0 3.032860671990308 9.159216026063465E-5 62-63 4.2743008121629505E-4 6.029817217158448E-4 0.0 3.3336416936062108 9.159216026063465E-5 64-65 4.2743008121629505E-4 6.18247081759284E-4 0.0 3.6559545055633844 9.159216026063465E-5 66-67 4.2743008121629505E-4 6.411451218244425E-4 0.0 4.0037223053929925 9.159216026063465E-5 68-69 4.2743008121629505E-4 6.564104818678817E-4 0.0 4.37967759254281 9.159216026063465E-5 70-71 4.2743008121629505E-4 7.098392420199185E-4 0.0 4.802390677505683 9.159216026063465E-5 72-73 4.3506276123801463E-4 7.556353221502359E-4 0.0 5.216005607882666 1.2212288034751286E-4 74-75 4.4269544125973416E-4 7.632680021719554E-4 0.0 5.622766391600144 1.2212288034751286E-4 76-77 4.4269544125973416E-4 8.090640823022727E-4 0.0 6.059203035242069 1.2212288034751286E-4 78-79 4.4269544125973416E-4 9.617176827366638E-4 0.0 6.514339744937205 1.2212288034751286E-4 80-81 4.5796080130317327E-4 0.0010533098429972984 0.0 7.013326201357121 1.2975556036923241E-4 82-83 4.6559348132489285E-4 0.0010838405630841767 0.0 7.549987566364244 1.5265360043439108E-4 84-85 4.732261613466124E-4 0.001114371283171055 0.0 8.068162580358763 1.5265360043439108E-4 86-87 4.732261613466124E-4 0.0011601673633013722 0.0 8.604365984564584 1.5265360043439108E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 2065 0.0 23.644339 1 GTACATA 1700 0.0 21.33554 1 TAGGCAT 3245 0.0 19.631367 5 CTAGGCA 3550 0.0 17.944729 4 GTATAAG 2535 0.0 17.609669 1 GTACAAA 2940 0.0 17.398159 1 CATGGGG 16580 0.0 17.331999 4 GTATATA 1670 0.0 16.985226 1 TACATGA 2395 0.0 16.502817 2 GTACAAG 2885 0.0 16.440397 1 GTACATG 53115 0.0 16.35376 1 ATGGGTA 3350 0.0 16.101187 5 TACATGG 52375 0.0 15.927334 2 TACTAGG 3020 0.0 15.704999 2 GTATCAA 37960 0.0 15.679843 1 CGTTAAC 595 0.0 15.630431 1 CATGGGT 4870 0.0 15.467878 4 TAGTACT 2615 0.0 15.292252 4 ACATGGG 49070 0.0 15.247009 3 GTCCTAT 3545 0.0 15.084799 1 >>END_MODULE