FastQCFastQC Report
Fri 27 May 2016
ERR765436_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765436_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6550779
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194910.2975371326066717No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT181740.27743265342946233No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA176820.26992209628809033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119790.18286374796035706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99600.15204298603265354No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG70060.10694911246433439No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG6450.036.047082
TCACGCA9600.026.1566624
GTATCAA466000.025.419541
AGTACTT275050.022.0138312-13
CAACGCA560150.020.8533155
ATCACGC11600.020.8451563
ATCAACG562600.020.8120963
AACGCAG579250.020.3736486
TCAACGC580300.020.1853084
TATCAAC589850.020.0950012
TACTTTT312500.019.45768714-15
GAGTACT261250.019.26048512-13
GTACTAG21500.019.2595791
TAACGCA3405.820766E-1119.147364
ACGCAGA618050.018.9065447
CGCAGAG622550.018.7773488
ACGTTAA7100.018.3376188
GCAGAGT655650.017.7662379
GTACTTT294950.017.10727914-15
GACGTTA7450.016.851977
Produced by FastQC (version 0.11.5)