##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765436_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6550779 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.377142626853995 33.0 33.0 33.0 33.0 33.0 2 32.27068353244706 33.0 33.0 33.0 33.0 33.0 3 32.321593355538326 33.0 33.0 33.0 33.0 33.0 4 36.23345712013793 37.0 37.0 37.0 33.0 37.0 5 36.16915209626214 37.0 37.0 37.0 33.0 37.0 6 36.19375741419456 37.0 37.0 37.0 33.0 37.0 7 36.09309427168891 37.0 37.0 37.0 33.0 37.0 8 36.101487166640794 37.0 37.0 37.0 33.0 37.0 9 36.12071724599471 37.0 37.0 37.0 33.0 37.0 10-11 36.07926362650915 37.0 37.0 37.0 33.0 37.0 12-13 36.05624445581205 37.0 37.0 37.0 33.0 37.0 14-15 37.5396068925543 40.0 37.0 40.0 33.0 40.0 16-17 37.53472602266082 40.0 37.0 40.0 33.0 40.0 18-19 37.58312164095293 40.0 37.0 40.0 33.0 40.0 20-21 37.57306734054072 40.0 37.0 40.0 33.0 40.0 22-23 37.54110083701495 40.0 37.0 40.0 33.0 40.0 24-25 37.51921206622907 40.0 37.0 40.0 33.0 40.0 26-27 37.397386249788 40.0 37.0 40.0 33.0 40.0 28-29 37.29406037052998 37.0 37.0 40.0 33.0 40.0 30-31 37.145827618364166 37.0 37.0 40.0 33.0 40.0 32-33 37.03563820425021 37.0 37.0 40.0 33.0 40.0 34-35 36.938191625759316 37.0 37.0 40.0 33.0 40.0 36-37 36.79372834894903 37.0 37.0 40.0 33.0 40.0 38-39 36.67619713624899 37.0 37.0 40.0 33.0 40.0 40-41 36.51866739818272 37.0 37.0 40.0 33.0 40.0 42-43 36.36458122003505 37.0 37.0 40.0 33.0 40.0 44-45 36.08744479091723 37.0 37.0 40.0 33.0 40.0 46-47 35.850954825372675 37.0 37.0 40.0 30.0 40.0 48-49 35.590170955240595 37.0 37.0 40.0 27.0 40.0 50-51 35.39425585873069 37.0 35.0 40.0 27.0 40.0 52-53 35.2435353108386 37.0 33.0 40.0 27.0 40.0 54-55 35.042798955666186 37.0 33.0 40.0 27.0 40.0 56-57 34.834423127386835 37.0 33.0 40.0 27.0 40.0 58-59 34.62875690051519 37.0 33.0 38.5 27.0 40.0 60-61 34.46388360834643 37.0 33.0 37.0 27.0 40.0 62-63 34.245731461861254 37.0 33.0 37.0 27.0 40.0 64-65 34.028467225043 37.0 33.0 37.0 27.0 40.0 66-67 33.80050571084752 37.0 33.0 37.0 27.0 40.0 68-69 33.592881625223505 37.0 33.0 37.0 27.0 40.0 70-71 33.401389666786194 37.0 33.0 37.0 27.0 37.0 72-73 33.19394372791389 37.0 33.0 37.0 27.0 37.0 74-75 32.966054800505404 37.0 33.0 37.0 27.0 37.0 76-77 32.789880867603685 37.0 33.0 37.0 27.0 37.0 78-79 32.633983607140465 33.0 33.0 37.0 24.5 37.0 80-81 32.46963040578838 33.0 33.0 37.0 22.0 37.0 82-83 32.380974995492906 33.0 33.0 37.0 22.0 37.0 84-85 32.252314114092385 33.0 33.0 37.0 22.0 37.0 86-87 32.14268829707123 33.0 33.0 37.0 22.0 37.0 88-89 32.031092256356075 33.0 33.0 37.0 22.0 37.0 90-91 31.92984590076997 33.0 33.0 37.0 22.0 37.0 92-93 31.853681447656836 33.0 33.0 37.0 22.0 37.0 94-95 31.75941540693099 33.0 33.0 37.0 22.0 37.0 96-97 31.70901758401558 33.0 33.0 37.0 22.0 37.0 98-99 30.91455703207206 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.021663476795374947 1101 2 0.0869046192411318 1101 3 0.0602528838703833 1101 4 0.07452569982022794 1101 5 0.08520345503032445 1101 6 0.038188200339085654 1101 7 0.05118796077314158 1101 8 0.11218389687782349 1101 9 0.0659686113468041 1101 10-11 0.06614998747888023 1101 12-13 0.08985933179594241 1101 14-15 0.15660709481932145 1101 16-17 0.17570189161502725 1101 18-19 0.1586547882134255 1101 20-21 0.20482570449886595 1101 22-23 0.1648625422777812 1101 24-25 0.20440414345264202 1101 26-27 0.1913677760458654 1101 28-29 0.15278962597789558 1101 30-31 0.15076267747125627 1101 32-33 0.21035495970792795 1101 34-35 0.2592168990654642 1101 36-37 0.23832549111097734 1101 38-39 0.18664772175007727 1101 40-41 0.09228639933184013 1101 42-43 0.21991509179739666 1101 44-45 0.252512386071416 1101 46-47 0.2555385816383975 1101 48-49 0.20046029631316742 1101 50-51 0.24506274386723703 1101 52-53 0.2776124241180753 1101 54-55 0.23869131019425538 1101 56-57 0.2432015874483966 1101 58-59 0.27856303634222 1101 60-61 0.2025989140104656 1101 62-63 0.19814680679046148 1101 64-65 0.17262522267543545 1101 66-67 0.29584390720716414 1101 68-69 0.3214291910929674 1101 70-71 0.15267769965541333 1101 72-73 0.20374399189322645 1101 74-75 0.22373793263648878 1101 76-77 0.23724464939451906 1101 78-79 0.2625274791085346 1101 80-81 0.27344833074905495 1101 82-83 0.24463204669172 1101 84-85 0.2277925984597644 1101 86-87 0.20177844577786885 1101 88-89 0.2771700950663387 1101 90-91 0.26679865924914736 1101 92-93 0.20316724430412947 1101 94-95 0.318237208300733 1101 96-97 0.2727933363756776 1101 98-99 0.39550929870181406 1102 1 0.012426791723129327 1102 2 0.12158325910567669 1102 3 0.09391209425735525 1102 4 0.11400970555884271 1102 5 0.10631264418578468 1102 6 0.1513097025777057 1102 7 0.13748241807063977 1102 8 0.1342636297340789 1102 9 0.09266466998224132 1102 10-11 0.05463874012829706 1102 12-13 0.05469491074698141 1102 14-15 0.1170597515940841 1102 16-17 0.12211727695163432 1102 18-19 0.13665894112755694 1102 20-21 0.16648932092354585 1102 22-23 0.14120065845310137 1102 24-25 0.20912521197328005 1102 26-27 0.12989820250805195 1102 28-29 0.06642197892452373 1102 30-31 0.10355066490958365 1102 32-33 0.13132144993306127 1102 34-35 0.17477029531913502 1102 36-37 0.2129721501493691 1102 38-39 0.21981993678262768 1102 40-41 0.1965708945085538 1102 42-43 0.20239642969583826 1102 44-45 0.25399271653802913 1102 46-47 0.2823386987584726 1102 48-49 0.3244554625995093 1102 50-51 0.2798609691869842 1102 52-53 0.292486706281224 1102 54-55 0.16438843310113782 1102 56-57 0.2463693929859474 1102 58-59 0.31291314032662143 1102 60-61 0.20513407524174454 1102 62-63 0.21819039713970767 1102 64-65 0.19464546634659996 1102 66-67 0.21617060187053028 1102 68-69 0.2501608849593069 1102 70-71 0.15000797649069852 1102 72-73 0.134713411354916 1102 74-75 0.32646063477140785 1102 76-77 0.3189972723001091 1102 78-79 0.3053270692788246 1102 80-81 0.21305970292043952 1102 82-83 0.2997542944718745 1102 84-85 0.2757397703758997 1102 86-87 0.2689016424215893 1102 88-89 0.34193654144139884 1102 90-91 0.25076512770153414 1102 92-93 0.23284267546721438 1102 94-95 0.26971265739237893 1102 96-97 0.3377960520006411 1102 98-99 0.2989842192796601 1103 1 -0.012785364470587979 1103 2 0.07380887469046371 1103 3 0.06284096880969514 1103 4 0.0677738416943825 1103 5 0.01913163954750985 1103 6 0.06297978493169154 1103 7 0.029278801704073487 1103 8 0.14348263678586193 1103 9 0.076722238621727 1103 10-11 0.11820017561807106 1103 12-13 0.07798758461520805 1103 14-15 0.1201818176648004 1103 16-17 0.17196658486397354 1103 18-19 0.17317760076558386 1103 20-21 0.11054690782874133 1103 22-23 0.11243146880513422 1103 24-25 0.1646181668898592 1103 26-27 0.16431405300201618 1103 28-29 0.13256606369688484 1103 30-31 0.14327139441758874 1103 32-33 0.09073209470036403 1103 34-35 0.17419303807344022 1103 36-37 0.15867934355033242 1103 38-39 0.09933311914380738 1103 40-41 0.07593795120897084 1103 42-43 -0.001479066370336568 1103 44-45 0.10478757924688864 1103 46-47 0.08461972032296217 1103 48-49 0.020074189090024674 1103 50-51 0.045562232321550766 1103 52-53 -0.06116789649443177 1103 54-55 -0.06610162974507006 1103 56-57 0.028038507257427625 1103 58-59 0.08439507371523547 1103 60-61 0.06091842908016076 1103 62-63 0.10461039151387297 1103 64-65 0.06424131321177384 1103 66-67 0.14047589612499678 1103 68-69 0.16309102715175783 1103 70-71 -0.0666459041806533 1103 72-73 -0.007936741894368993 1103 74-75 0.09546025849783035 1103 76-77 0.11746834124585348 1103 78-79 0.084063197996052 1103 80-81 0.1293048579414986 1103 82-83 0.03019975854870438 1103 84-85 0.03759549320475486 1103 86-87 0.02131819553644476 1103 88-89 0.08894233532384987 1103 90-91 0.06787720364790673 1103 92-93 0.012946662140560505 1103 94-95 0.055992797332052646 1103 96-97 -0.01239388091550353 1103 98-99 0.10640438900461291 1104 1 -0.033345336025242034 1104 2 0.026202497532416658 1104 3 -0.07478252910337346 1104 4 -0.07188291256511548 1104 5 -0.04906778207858764 1104 6 0.009081166390032536 1104 7 0.04909196061112908 1104 8 0.09934185102270732 1104 9 -0.00439792232700853 1104 10-11 -0.013407971129161922 1104 12-13 -0.041015588206086306 1104 14-15 0.0379791626499042 1104 16-17 0.04146913424710874 1104 18-19 0.018047481907700558 1104 20-21 0.008326192498977036 1104 22-23 0.07828613386223537 1104 24-25 0.02525957782167154 1104 26-27 0.024045052847391446 1104 28-29 0.07251831614144066 1104 30-31 -0.04714902450801617 1104 32-33 0.04928737232400948 1104 34-35 0.0030107531840357638 1104 36-37 0.047113372147769894 1104 38-39 0.0012892766148482337 1104 40-41 0.023737223156977905 1104 42-43 0.13846223302564198 1104 44-45 0.053384835752524396 1104 46-47 0.08921111918708391 1104 48-49 0.07442492626785224 1104 50-51 0.025854435430559874 1104 52-53 0.13075612864098218 1104 54-55 0.020654750125792987 1104 56-57 0.1329271704347974 1104 58-59 0.11407248607583398 1104 60-61 0.1584832844118651 1104 62-63 0.05420800440076334 1104 64-65 0.1052693158612783 1104 66-67 0.18284065931265303 1104 68-69 0.14770147475174866 1104 70-71 -0.04129624388293962 1104 72-73 0.11539835145950406 1104 74-75 0.12707463504592909 1104 76-77 0.11102447750299405 1104 78-79 0.0583032055532442 1104 80-81 0.11911708237841623 1104 82-83 0.11008268817160172 1104 84-85 0.18334199487127023 1104 86-87 0.25742235495663834 1104 88-89 0.15716290468083116 1104 90-91 0.15934340967576333 1104 92-93 0.14489479619813395 1104 94-95 0.11179602479643691 1104 96-97 0.07730828370616649 1104 98-99 0.19500243339683365 1105 1 -0.010682565074318973 1105 2 0.0512197784481927 1105 3 0.01863089982305155 1105 4 0.030645866064475058 1105 5 0.0548671748281393 1105 6 0.02975152627320199 1105 7 0.04595036549349629 1105 8 0.04553576177801233 1105 9 0.014557559684625687 1105 10-11 -0.03050389502104167 1105 12-13 0.01775262317691073 1105 14-15 0.006256987243375534 1105 16-17 0.034010317425817504 1105 18-19 0.02573058386673921 1105 20-21 0.050490578360360416 1105 22-23 0.04834972954803618 1105 24-25 0.040248225680443284 1105 26-27 0.005138251317909237 1105 28-29 -0.0025907070148036837 1105 30-31 0.04036049442688494 1105 32-33 0.020227816624156958 1105 34-35 0.03708203913573982 1105 36-37 0.0563988413817853 1105 38-39 -0.003070238022189642 1105 40-41 0.0899059265375115 1105 42-43 0.08866656652627825 1105 44-45 0.07918293526770981 1105 46-47 0.0365233198337549 1105 48-49 0.034013057930003754 1105 50-51 0.02920873037651006 1105 52-53 -0.02740208443847081 1105 54-55 -0.023822684610422584 1105 56-57 -0.046283894191113006 1105 58-59 0.02830591594501186 1105 60-61 -0.04248351419860086 1105 62-63 -0.05093337746248494 1105 64-65 -0.002041770619428007 1105 66-67 0.13387648327745438 1105 68-69 0.028224517695285556 1105 70-71 -0.002289222872846608 1105 72-73 -0.02777400673830499 1105 74-75 0.06243391197742909 1105 76-77 0.030320450650080488 1105 78-79 0.04337071972545914 1105 80-81 0.010121271087882633 1105 82-83 -0.0010242710047947412 1105 84-85 -0.02147618811561358 1105 86-87 0.10409920876369227 1105 88-89 0.11300089486658749 1105 90-91 0.09184932269570822 1105 92-93 0.18021909033492634 1105 94-95 0.04472414117787693 1105 96-97 0.036649095731497994 1105 98-99 0.07841748315745178 1106 1 0.014756849978752484 1106 2 0.0893805710697535 1106 3 0.08650379194767055 1106 4 0.06749126487785873 1106 5 0.0824354120055375 1106 6 0.09348850055995683 1106 7 0.02451807857242727 1106 8 0.04712703669294882 1106 9 0.004310015260287514 1106 10-11 0.007119905434493035 1106 12-13 -0.02335088651448558 1106 14-15 0.04154741981862742 1106 16-17 0.08979560307912493 1106 18-19 0.027144903101152806 1106 20-21 0.03385066838544759 1106 22-23 0.07495497141677276 1106 24-25 0.06157022106621213 1106 26-27 0.07030528252853685 1106 28-29 0.036343113643319214 1106 30-31 0.053244941768248566 1106 32-33 0.08864486244765146 1106 34-35 0.11848052735481929 1106 36-37 0.09416279765222413 1106 38-39 0.007705781354225394 1106 40-41 -0.04664326086427906 1106 42-43 -0.0484447078778274 1106 44-45 0.061037344939805394 1106 46-47 0.023764494030423577 1106 48-49 0.056377355969615905 1106 50-51 0.03649406735905103 1106 52-53 0.011599908314529728 1106 54-55 -0.010620668968023494 1106 56-57 0.13073499742169048 1106 58-59 0.13658833673958526 1106 60-61 -0.02252849235789256 1106 62-63 -0.02178686713793354 1106 64-65 0.03611934618673729 1106 66-67 0.08910838579416236 1106 68-69 0.042199355074949096 1106 70-71 -0.06387814499338162 1106 72-73 -0.0024030781409436486 1106 74-75 0.01734174285326162 1106 76-77 0.09040220902743101 1106 78-79 -0.025142952760646153 1106 80-81 0.055048238679738404 1106 82-83 -0.12422647821083643 1106 84-85 -0.03945152749023606 1106 86-87 0.041850983551661614 1106 88-89 0.11851937658232714 1106 90-91 -0.0028579066703144917 1106 92-93 0.137092152902067 1106 94-95 0.0770170301883617 1106 96-97 0.0878159491317767 1106 98-99 0.13466351864977 1107 1 -0.010038696205093345 1107 2 0.047384260195237005 1107 3 -0.0015935610211244011 1107 4 -0.011992332651267645 1107 5 -0.012446923346111305 1107 6 0.02142725922062283 1107 7 0.05718974187900727 1107 8 -1.5573828208204077E-4 1107 9 0.023400083349073952 1107 10-11 -0.06799720349391691 1107 12-13 -0.1297584944245287 1107 14-15 -0.04725012872854961 1107 16-17 -0.10983588874221084 1107 18-19 -0.10653461782742824 1107 20-21 -0.08853706516158155 1107 22-23 -0.005234415476046195 1107 24-25 -0.03033713514273728 1107 26-27 -0.05978202692870127 1107 28-29 -0.09603635284743461 1107 30-31 -0.10423785426097965 1107 32-33 -0.009845016738722734 1107 34-35 -0.019844853721082245 1107 36-37 -0.031185126648090034 1107 38-39 -0.10046878876626408 1107 40-41 -0.1966650641108032 1107 42-43 -0.1155317020997515 1107 44-45 -0.12005230345140916 1107 46-47 -0.02352508783999241 1107 48-49 -0.09478304254126613 1107 50-51 -0.04116046346359781 1107 52-53 -0.06285126576709388 1107 54-55 -0.1675473840067241 1107 56-57 -0.15462022903101058 1107 58-59 -0.061899360774226864 1107 60-61 -0.043859256580844885 1107 62-63 -0.07796243693813665 1107 64-65 -0.08938422687492675 1107 66-67 -0.08161462568881461 1107 68-69 -0.18394190855017456 1107 70-71 -0.027278426469578676 1107 72-73 -0.1676114597379197 1107 74-75 -0.030144598730849737 1107 76-77 -0.06712872629024957 1107 78-79 -0.0024469679450902504 1107 80-81 -0.12693135592314064 1107 82-83 -0.15794514215764366 1107 84-85 -0.07139688446838477 1107 86-87 -0.04088110611535001 1107 88-89 -0.15508014897994826 1107 90-91 -0.12911089773279016 1107 92-93 0.07800413208093815 1107 94-95 -0.07798594741918663 1107 96-97 -0.08649990926115336 1107 98-99 -0.03296258168679245 1108 1 -0.03407722290015869 1108 2 0.03550664694672889 1108 3 0.04327891762900293 1108 4 -0.014795145891618233 1108 5 0.05724451613220083 1108 6 0.048629331759485694 1108 7 -0.06138740379046226 1108 8 -0.06768390517609646 1108 9 0.004085628142767916 1108 10-11 -0.03043980777032118 1108 12-13 -0.006667765687375038 1108 14-15 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66-67 -0.16002230073031853 2215 68-69 -0.10524262171696819 2215 70-71 -0.22015504813910525 2215 72-73 -0.21643205695163203 2215 74-75 -0.2643030265755826 2215 76-77 -0.21964626350496985 2215 78-79 -0.16292373295975437 2215 80-81 -0.1828910626222111 2215 82-83 -0.13623382988603083 2215 84-85 -0.08385320188876477 2215 86-87 -0.12670584100357019 2215 88-89 -0.13786723718402172 2215 90-91 -0.21826847320949483 2215 92-93 -0.0717050671633288 2215 94-95 -0.09353190164856073 2215 96-97 -0.20369333925101074 2215 98-99 -0.16367967293070862 2216 1 -0.059618897849126995 2216 2 -0.05705142633622273 2216 3 -0.06738079623519866 2216 4 -0.18376878384042072 2216 5 -0.13005834230427382 2216 6 -0.1660121471959144 2216 7 -0.11433589722178539 2216 8 -0.19658625974092558 2216 9 -0.12835406549723416 2216 10-11 -0.13567204795826626 2216 12-13 -0.11092280751406491 2216 14-15 -0.34001973770813265 2216 16-17 -0.29486465479542545 2216 18-19 -0.1876055633359286 2216 20-21 -0.26556741897780256 2216 22-23 -0.27662588221677 2216 24-25 -0.3313575037812413 2216 26-27 -0.3218126922421476 2216 28-29 -0.2456376306730803 2216 30-31 -0.2335769360702855 2216 32-33 -0.18693142338942437 2216 34-35 -0.2822207672960815 2216 36-37 -0.24250822998673272 2216 38-39 -0.28274021859441234 2216 40-41 -0.2069929238864603 2216 42-43 -0.2707496555497855 2216 44-45 -0.29936588249314866 2216 46-47 -0.2537001701713777 2216 48-49 -0.2088071239584508 2216 50-51 -0.2800180456341934 2216 52-53 -0.268399957699323 2216 54-55 -0.22574661480110336 2216 56-57 -0.34281698039330877 2216 58-59 -0.32783718676666496 2216 60-61 -0.3636206829432638 2216 62-63 -0.29126975271388744 2216 64-65 -0.3557559863812969 2216 66-67 -0.37201531151089995 2216 68-69 -0.16749138862613933 2216 70-71 -0.2980570231513866 2216 72-73 -0.3056158017840218 2216 74-75 -0.29716443130105574 2216 76-77 -0.299877146087681 2216 78-79 -0.16339237791498107 2216 80-81 -0.32323003609690204 2216 82-83 -0.34172771745016206 2216 84-85 -0.3584160156982321 2216 86-87 -0.4667309222266951 2216 88-89 -0.39476478023937744 2216 90-91 -0.33496833208146626 2216 92-93 -0.23052260071251496 2216 94-95 -0.3254947744246195 2216 96-97 -0.2873434267762889 2216 98-99 -0.36714839215695605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 27.0 10 38.0 11 118.0 12 250.0 13 485.0 14 1165.0 15 2924.0 16 6520.0 17 12466.0 18 20471.0 19 28212.0 20 36151.0 21 46690.0 22 56290.0 23 55382.0 24 48575.0 25 47104.0 26 55461.0 27 70696.0 28 92958.0 29 123008.0 30 161540.0 31 214186.0 32 282289.0 33 372411.0 34 496052.0 35 711489.0 36 1191470.0 37 1781210.0 38 634280.0 39 858.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01290924761809 18.66511118473074 13.155201326008639 25.16677824164253 2 16.213003064215723 20.979306430578713 36.84303805700055 25.964652448205015 3 18.232701790122977 25.877044546915716 29.393053864280873 26.49719979868043 4 13.299303792724498 17.25426853813875 36.135793926188015 33.310633742948745 5 14.365752786195815 36.88516719312154 33.073716353106576 15.675363667576075 6 31.959191418303075 36.60634864952703 17.116422336946492 14.318037595223407 7 27.46537472871547 31.703695087256033 22.302553635224147 18.52837654880435 8 27.574354134065583 31.916753717382313 20.140520692271867 20.368371456280236 9 27.515918335819293 14.034880431777655 19.819078005837167 38.63012322656588 10-11 25.793405639237715 24.76626825603489 26.919920516323327 22.520405588404067 12-13 26.911700119939937 22.780962386305507 26.93936095233865 23.367976541415914 14-15 24.52385715604006 23.428704762470314 25.167491987290187 26.879946094199443 16-17 23.665400877602554 25.778046562993023 26.010106619237934 24.546445940166485 18-19 24.072463569001364 24.514459435117253 28.53139492637473 22.881682069506653 20-21 25.134570171160934 23.95336452714794 27.633339589600574 23.27872571209055 22-23 24.274532845635612 23.688854409529004 27.659542475787998 24.37707026904739 24-25 23.963340848470082 24.329465243751926 27.640552367893957 24.066641539884035 26-27 24.231713510713764 24.20408320903514 27.48082327307943 24.083380007171666 28-29 23.761334339015253 24.01574682949921 28.18711331888925 24.03580551259629 30-31 24.33457731094143 23.94846186572827 27.796923136105868 23.92003768722443 32-33 23.877305279265258 24.087593246543655 27.33905387435601 24.696047599835072 34-35 24.351554219734783 24.065969864042124 27.82647682054302 23.75599909568007 36-37 24.46867006198805 24.11110190101055 27.247011385974098 24.173216651027303 38-39 24.497147591149083 23.704272423172878 27.362188527501846 24.436391458176196 40-41 24.25982467123376 24.055764970853087 27.53071046970139 24.15369988821177 42-43 25.057325243303126 23.908935105275265 26.967082846177533 24.06665680524408 44-45 24.630582103288784 24.255893841022573 26.5093128618749 24.604211193813743 46-47 24.312818368624555 24.241399381661328 26.810551844292107 24.63523040542201 48-49 25.197758923022747 24.59354070714338 25.87757883451724 24.331121535316637 50-51 24.361369846242713 25.0301529024258 25.594055302430448 25.014421948901038 52-53 24.389603129643056 24.782815906321982 25.45487338223439 25.372707581800576 54-55 24.650198090944603 24.775328247220674 25.50557727561867 25.06889638621605 56-57 24.731837236456915 24.310780443058757 25.307516861735067 25.649865458749254 58-59 24.29448074717232 24.387439171347282 25.980738471064047 25.337341610416352 60-61 24.38522197131059 24.59846378575739 25.761806343947796 25.25450789898423 62-63 23.983216347246643 24.653617531594335 25.64327845589051 25.719887665268516 64-65 24.043926684139397 24.33738033293445 25.802770937624363 25.815922045301786 66-67 24.609012149547404 24.398533365268467 25.677304943427337 25.31514954175679 68-69 24.613332246439697 24.023265019320604 25.671778883091612 25.691623851148083 70-71 23.952510075519264 24.15020412076182 25.92946579330489 25.967820010414027 72-73 24.61448478112298 24.243154898066322 25.7679582840453 25.374402036765396 74-75 24.835126453682296 24.113586203043365 25.655143556994297 25.39614378628004 76-77 24.35870956356926 24.375539630724667 25.817735181297607 25.448015624408466 78-79 24.37366795412179 24.475297096368013 25.725866017298554 25.425168932211644 80-81 23.95197761609555 24.32339148698128 26.05401022183852 25.67062067508465 82-83 24.15571675946607 24.552753539140422 25.48342918326425 25.808100518129258 84-85 24.131683527583327 24.58153271281415 25.19470668324899 26.092077076353537 86-87 23.948611113655364 24.268492177038357 25.487503961399028 26.295392747907254 88-89 23.961167849672346 23.90699090906662 25.735512267092776 26.396328974168252 90-91 24.231962496253264 24.081871792282524 25.551713475747 26.134452235717205 92-93 23.846489102268087 24.33692711056494 25.392276368465843 26.424307418701126 94-95 23.22922780032114 24.42228195621893 25.884665757910003 26.46382448554992 96-97 23.957786235111136 24.235933801779804 25.478106441038495 26.328173522070564 98-99 23.61143847042531 24.17815341665425 25.56440222900144 26.646005883919 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 128.0 1 137.5 2 450.0 3 777.5 4 557.5 5 460.0 6 917.5 7 2478.0 8 4628.5 9 4602.0 10 2737.5 11 1676.0 12 1637.0 13 1841.5 14 2119.0 15 2589.5 16 3152.5 17 3733.5 18 4485.5 19 5498.5 20 6718.5 21 8197.5 22 9919.0 23 11897.5 24 14493.0 25 17916.5 26 21939.5 27 25910.0 28 31110.5 29 38254.0 30 44179.5 31 50513.0 32 58736.0 33 67316.5 34 75184.5 35 83093.0 36 88957.5 37 96561.5 38 106948.5 39 108664.5 40 110949.5 41 119477.5 42 132771.0 43 153698.0 44 174398.5 45 197667.5 46 233806.0 47 265519.5 48 286736.0 49 308078.0 50 332134.0 51 323374.5 52 298933.5 53 296817.0 54 288399.5 55 278094.5 56 256682.0 57 240186.0 58 231872.5 59 205359.5 60 170941.5 61 132180.0 62 108647.0 63 88098.0 64 64503.0 65 50117.5 66 36338.0 67 26882.5 68 21124.5 69 18116.0 70 16532.0 71 14980.0 72 15418.0 73 13067.5 74 8326.5 75 6395.5 76 4895.5 77 3174.0 78 1677.0 79 1038.5 80 741.0 81 539.0 82 382.5 83 263.5 84 162.5 85 105.0 86 56.5 87 26.0 88 16.0 89 9.5 90 7.0 91 5.5 92 2.5 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.055138480476902056 2 0.0 3 0.0 4 0.0 5 0.005495529615638079 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014120458040181175 16-17 0.0048772825338787955 18-19 5.953490416941253E-4 20-21 2.2134772062986708E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.7477648078190394E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.526536004343911E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.526536004343911E-5 76-77 4.5796080130317326E-5 78-79 7.632680021719555E-6 80-81 7.632680021719555E-6 82-83 7.632680021719555E-6 84-85 4.2743008121629505E-4 86-87 9.617176827366639E-4 88-89 3.282052409339408E-4 90-91 0.0011220039631927746 92-93 0.0015875974445176673 94-95 0.0010762078830624572 96-97 0.0018242105251909735 98-99 6.564104818678816E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6550779.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.748921286552104 #Duplication Level Percentage of deduplicated Percentage of total 1 72.53744884069923 27.382104466148554 2 14.86105145744519 11.21977323404998 3 5.077088137379169 5.749638013884413 4 2.3158830439640123 3.4968834694183286 5 1.284152767623052 2.4237690872455318 6 0.7824109283475087 1.772110112875696 7 0.5405812848337895 1.4284452259121747 8 0.3767982306669815 1.137898140028799 9 0.27688834275146684 0.9407012630120085 >10 1.6064149949571196 11.238307747613895 >50 0.15756422985783178 4.17081843728293 >100 0.14763312949824217 11.726678014672833 >500 0.02123815918701915 5.507094869821077 >1k 0.014199195992047942 9.67283190525719 >5k 4.854425980137863E-4 1.104825567054644 >10k+ 1.618141993379288E-4 1.0281204457217235 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19491 0.2975371326066717 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 18174 0.27743265342946233 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 17682 0.26992209628809033 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11979 0.18286374796035706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9960 0.15204298603265354 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 7006 0.10694911246433439 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5265360043439107E-5 2 0.0 0.0 0.0 0.0 1.5265360043439107E-5 3 0.0 0.0 0.0 0.0 1.5265360043439107E-5 4 0.0 1.5265360043439107E-5 0.0 0.0 1.5265360043439107E-5 5 0.0 1.5265360043439107E-5 0.0 1.5265360043439107E-5 1.5265360043439107E-5 6 0.0 1.5265360043439107E-5 0.0 1.5265360043439107E-5 1.5265360043439107E-5 7 0.0 1.5265360043439107E-5 0.0 1.5265360043439107E-5 1.5265360043439107E-5 8 0.0 1.5265360043439107E-5 0.0 1.5265360043439107E-5 1.5265360043439107E-5 9 0.0 1.5265360043439107E-5 0.0 3.0530720086878215E-5 3.0530720086878215E-5 10-11 1.5265360043439107E-5 1.5265360043439107E-5 0.0 6.106144017375643E-5 3.0530720086878215E-5 12-13 1.5265360043439107E-5 1.5265360043439107E-5 0.0 9.92248402823542E-5 3.0530720086878215E-5 14-15 1.5265360043439107E-5 3.0530720086878215E-5 0.0 1.0685752030407376E-4 3.816340010859777E-5 16-17 1.5265360043439107E-5 3.0530720086878215E-5 0.0 1.7555164049954975E-4 6.106144017375643E-5 18-19 1.5265360043439107E-5 5.3428760152036874E-5 0.0 2.1371504060814752E-4 6.106144017375643E-5 20-21 3.0530720086878215E-5 6.106144017375643E-5 0.0 3.58735961020819E-4 6.106144017375643E-5 22-23 3.816340010859777E-5 6.106144017375643E-5 0.0 0.0014578418841484347 6.869412019547598E-5 24-25 7.632680021719554E-5 1.2212288034751286E-4 0.0 0.00377054393072946 7.632680021719554E-5 26-27 9.92248402823542E-5 1.2212288034751286E-4 0.0 0.006365655138114108 7.632680021719554E-5 28-29 1.144902003257933E-4 1.2212288034751286E-4 0.0 0.01747120456971606 7.632680021719554E-5 30-31 1.2212288034751286E-4 1.2212288034751286E-4 0.0 0.050826016264630514 7.632680021719554E-5 32-33 1.2212288034751286E-4 1.3738824039095197E-4 0.0 0.10616294642209728 7.632680021719554E-5 34-35 1.2212288034751286E-4 1.9081700054298886E-4 0.0 0.17466624961703028 7.632680021719554E-5 36-37 1.2212288034751286E-4 1.984496805647084E-4 0.0 0.28230230328331946 7.632680021719554E-5 38-39 1.2212288034751286E-4 2.0608236058642797E-4 0.0 0.44133682421586806 7.632680021719554E-5 40-41 1.2212288034751286E-4 2.1371504060814752E-4 0.0 0.6357564497291086 7.632680021719554E-5 42-43 1.2212288034751286E-4 2.2134772062986708E-4 0.0 0.8095907372237714 7.632680021719554E-5 44-45 1.3738824039095197E-4 2.366130806733062E-4 0.0 1.0081014792286536 7.632680021719554E-5 46-47 1.5265360043439108E-4 2.442457606950257E-4 0.0 1.223892608802709 9.159216026063465E-5 48-49 1.6028628045611064E-4 2.5951112073846483E-4 0.0 1.448965077283175 9.159216026063465E-5 50-51 1.679189604778302E-4 2.7477648078190394E-4 0.0 1.6939588406203292 9.159216026063465E-5 52-53 1.7555164049954975E-4 2.976745208470626E-4 0.0 1.9421583295666056 1.144902003257933E-4 54-55 1.831843205212693E-4 3.0530720086878216E-4 0.0 2.1867322955025656 1.3738824039095197E-4 56-57 1.831843205212693E-4 3.2057256091222127E-4 0.0 2.4607455082822973 1.3738824039095197E-4 58-59 1.831843205212693E-4 3.511032809990995E-4 0.0 2.755947040802323 1.3738824039095197E-4 60-61 1.984496805647084E-4 3.816340010859777E-4 0.0 3.0548504231328826 1.4502092041267153E-4 62-63 1.984496805647084E-4 3.816340010859777E-4 0.0 3.3585547611971034 1.5265360043439108E-4 64-65 1.984496805647084E-4 3.8926668110769724E-4 0.0 3.684386238644289 1.5265360043439108E-4 66-67 1.984496805647084E-4 4.1216472117285594E-4 0.0 4.035924582404627 1.5265360043439108E-4 68-69 2.1371504060814752E-4 4.1216472117285594E-4 0.0 4.415444331124588 1.5265360043439108E-4 70-71 2.1371504060814752E-4 4.961242014117711E-4 0.0 4.843149188821665 1.5265360043439108E-4 72-73 2.1371504060814752E-4 5.495529615638079E-4 0.0 5.261259767731441 1.679189604778302E-4 74-75 2.1371504060814752E-4 5.724510016289666E-4 0.0 5.671920850940018 1.679189604778302E-4 76-77 2.1371504060814752E-4 6.33512441802723E-4 0.0 6.113883554917667 1.679189604778302E-4 78-79 2.2898040065158663E-4 7.251046020633577E-4 0.0 6.5751035105901146 1.679189604778302E-4 80-81 2.2898040065158663E-4 7.632680021719554E-4 0.0 7.079791578986255 1.679189604778302E-4 82-83 2.366130806733062E-4 7.70900682193675E-4 0.0 7.623246029212709 1.679189604778302E-4 84-85 2.442457606950257E-4 7.937987222588337E-4 0.0 8.148061474826124 1.679189604778302E-4 86-87 2.442457606950257E-4 8.090640823022727E-4 0.0 8.691096127651385 1.679189604778302E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 645 0.0 36.04708 2 TCACGCA 960 0.0 26.156662 4 GTATCAA 46600 0.0 25.41954 1 AGTACTT 27505 0.0 22.01383 12-13 CAACGCA 56015 0.0 20.853315 5 ATCACGC 1160 0.0 20.845156 3 ATCAACG 56260 0.0 20.812096 3 AACGCAG 57925 0.0 20.373648 6 TCAACGC 58030 0.0 20.185308 4 TATCAAC 58985 0.0 20.095001 2 TACTTTT 31250 0.0 19.457687 14-15 GAGTACT 26125 0.0 19.260485 12-13 GTACTAG 2150 0.0 19.259579 1 TAACGCA 340 5.820766E-11 19.14736 4 ACGCAGA 61805 0.0 18.906544 7 CGCAGAG 62255 0.0 18.777348 8 ACGTTAA 710 0.0 18.337618 8 GCAGAGT 65565 0.0 17.766237 9 GTACTTT 29495 0.0 17.107279 14-15 GACGTTA 745 0.0 16.85197 7 >>END_MODULE