FastQCFastQC Report
Fri 27 May 2016
ERR765435_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765435_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7420636
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA96310.1297867190898462No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT91380.1231430836925568No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG24950.024.4140171
GTATCAA247950.023.103891
GTATAAG30200.020.1698591
GTACATG399050.019.6923681
TAGTACT34250.019.4127754
CAACGCA276250.019.1536755
TACATGG395000.018.8936752
ATCAACG281750.018.8634363
AACGCAG289850.018.639466
CTAGTAC36650.018.3963873
CATGGGG172950.018.388564
TATCAAC310450.018.3627322
TCAACGC300200.017.7959634
ACATGGG392300.017.4472923
ACGCAGA301650.017.3400237
TAGGCAT41400.017.1831685
CGCAGAG304800.016.9472648
GTATTAG29250.016.5327841
CTAGGCA44150.016.4288084
TATTCGC5100.016.4101129
Produced by FastQC (version 0.11.5)