##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765435_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7420636 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33550102713568 33.0 33.0 33.0 27.0 33.0 2 31.246869405802954 33.0 33.0 33.0 27.0 33.0 3 31.274870644510795 33.0 33.0 33.0 27.0 33.0 4 34.85717693200421 37.0 37.0 37.0 33.0 37.0 5 34.939046464480946 37.0 37.0 37.0 33.0 37.0 6 34.87762315790722 37.0 37.0 37.0 33.0 37.0 7 34.81520020655911 37.0 37.0 37.0 33.0 37.0 8 34.76914027315179 37.0 37.0 37.0 33.0 37.0 9 34.6574494423389 37.0 37.0 37.0 27.0 37.0 10-11 34.657371551441145 37.0 37.0 37.0 27.0 37.0 12-13 34.57805786458196 37.0 37.0 37.0 27.0 37.0 14-15 35.99852768684517 37.0 37.0 40.0 27.0 40.0 16-17 36.06217216421881 37.0 37.0 40.0 27.0 40.0 18-19 36.068693370756904 37.0 37.0 40.0 27.0 40.0 20-21 36.07624124131678 37.0 37.0 40.0 27.0 40.0 22-23 36.07751033738887 37.0 37.0 40.0 27.0 40.0 24-25 36.147787871551714 37.0 37.0 40.0 27.0 40.0 26-27 36.10846806122818 37.0 37.0 40.0 27.0 40.0 28-29 36.12080029258948 37.0 37.0 40.0 27.0 40.0 30-31 36.037042916537075 37.0 37.0 40.0 27.0 40.0 32-33 35.959480157765455 37.0 37.0 40.0 27.0 40.0 34-35 35.82219933709186 37.0 37.0 40.0 27.0 40.0 36-37 35.614377932026315 37.0 37.0 40.0 27.0 40.0 38-39 35.33153634001182 37.0 33.0 40.0 27.0 40.0 40-41 35.43825562930186 37.0 33.0 40.0 27.0 40.0 42-43 35.3613136394239 37.0 33.0 40.0 27.0 40.0 44-45 35.26461539145701 37.0 33.0 40.0 27.0 40.0 46-47 35.17179275469111 37.0 33.0 40.0 27.0 40.0 48-49 35.03594429102843 37.0 33.0 40.0 27.0 40.0 50-51 34.85816451581778 37.0 33.0 40.0 27.0 40.0 52-53 34.71224528463598 37.0 33.0 40.0 27.0 40.0 54-55 34.52218825987422 37.0 33.0 40.0 27.0 40.0 56-57 34.31907110118324 37.0 33.0 38.5 27.0 40.0 58-59 34.10426242440675 37.0 33.0 37.0 27.0 40.0 60-61 33.89282946906438 37.0 33.0 37.0 27.0 40.0 62-63 33.66710056927735 37.0 33.0 37.0 27.0 40.0 64-65 33.44076208562177 37.0 33.0 37.0 27.0 40.0 66-67 33.22535258433374 37.0 33.0 37.0 22.0 40.0 68-69 33.0210374825015 37.0 33.0 37.0 22.0 40.0 70-71 32.773418949534786 37.0 33.0 37.0 22.0 37.0 72-73 32.437603865760295 33.0 33.0 37.0 22.0 37.0 74-75 32.20166795676273 33.0 33.0 37.0 22.0 37.0 76-77 32.014025212933234 33.0 33.0 37.0 22.0 37.0 78-79 31.760751841216845 33.0 33.0 37.0 22.0 37.0 80-81 31.55869564279935 33.0 33.0 37.0 22.0 37.0 82-83 31.438597985401792 33.0 27.0 37.0 22.0 37.0 84-85 31.307045986354808 33.0 27.0 37.0 22.0 37.0 86-87 31.04952109226217 33.0 27.0 37.0 22.0 37.0 88-89 30.90738401667997 33.0 27.0 37.0 22.0 37.0 90-91 30.769878821707465 33.0 27.0 37.0 18.5 37.0 92-93 30.527501887978335 33.0 27.0 37.0 15.0 37.0 94-95 30.386426176947637 33.0 27.0 37.0 15.0 37.0 96-97 30.201208899075496 33.0 27.0 37.0 15.0 37.0 98-99 29.150758371654398 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27004922943100595 1101 2 0.33982582001786454 1101 3 0.3610478734528577 1101 4 0.2987292756220157 1101 5 0.23992842392070202 1101 6 0.4105677658564204 1101 7 0.31860624735579535 1101 8 0.3985497831526956 1101 9 0.37142159235217065 1101 10-11 0.3306507593954322 1101 12-13 0.4298347472609052 1101 14-15 0.5614703645028953 1101 16-17 0.538243446533194 1101 18-19 0.4265096422774448 1101 20-21 0.4334537576685378 1101 22-23 0.44777587733076274 1101 24-25 0.3235670284308796 1101 26-27 0.4520302984356519 1101 28-29 0.4340822215886959 1101 30-31 0.6400024413550724 1101 32-33 0.6822965459277484 1101 34-35 0.7413718201050017 1101 36-37 0.8287932195732566 1101 38-39 0.9043634418827224 1101 40-41 0.59206848553859 1101 42-43 0.5520258245866572 1101 44-45 0.41761190903812206 1101 46-47 0.44016773026311995 1101 48-49 0.34665467100683145 1101 50-51 0.2758096804029009 1101 52-53 0.4163559389399154 1101 54-55 0.33636352286531945 1101 56-57 0.4494984946799079 1101 58-59 0.3613176887461549 1101 60-61 0.14937050517464456 1101 62-63 0.3419177805613529 1101 64-65 0.4389306374010218 1101 66-67 0.4278501751421686 1101 68-69 0.5161912568686091 1101 70-71 0.45084383340341105 1101 72-73 0.2734066442366725 1101 74-75 0.3691112451612284 1101 76-77 0.29234304423430046 1101 78-79 0.37323092125367907 1101 80-81 0.34776042256469 1101 82-83 0.4511016900695175 1101 84-85 0.3864357189023444 1101 86-87 0.39858362475253273 1101 88-89 0.27923953156497205 1101 90-91 0.29253725360192817 1101 92-93 0.352725310896961 1101 94-95 0.44566731874473575 1101 96-97 0.36028011208353306 1101 98-99 0.261461523563554 1102 1 0.17156546020721208 1102 2 0.20346754519903953 1102 3 0.173028043028431 1102 4 0.26351915597955156 1102 5 0.2459756074076651 1102 6 0.2322353428965016 1102 7 0.24542153657482402 1102 8 0.25224845570396326 1102 9 0.15822088808265988 1102 10-11 0.16384291718972577 1102 12-13 0.22079286530978948 1102 14-15 0.39071685292256575 1102 16-17 0.2288762921322629 1102 18-19 0.25579752154835234 1102 20-21 0.20907402064764113 1102 22-23 0.300083686069911 1102 24-25 0.1382365854645542 1102 26-27 0.26258529869819114 1102 28-29 0.22683423742056164 1102 30-31 0.38336774833241094 1102 32-33 0.4896828797119852 1102 34-35 0.5711763335399667 1102 36-37 0.6089024535683976 1102 38-39 0.5931350510382316 1102 40-41 0.4003340230299699 1102 42-43 0.30771039280576673 1102 44-45 0.2562052627870699 1102 46-47 0.2326514451102284 1102 48-49 0.11157338704878583 1102 50-51 0.17682359085410582 1102 52-53 0.2959259690276923 1102 54-55 0.15120417798842567 1102 56-57 0.2297163070963606 1102 58-59 0.1700445771743162 1102 60-61 0.1023793325907505 1102 62-63 0.1425698228475838 1102 64-65 0.16524968888143832 1102 66-67 0.23031630170049766 1102 68-69 0.23738344904793252 1102 70-71 0.28968623602360566 1102 72-73 0.14471560513648285 1102 74-75 0.2885201644022075 1102 76-77 0.19491384710238435 1102 78-79 0.17792815783266036 1102 80-81 0.03108082480233776 1102 82-83 0.057115922303776046 1102 84-85 0.2183070711745394 1102 86-87 0.261808952403662 1102 88-89 0.17219420193487522 1102 90-91 0.11190943160370992 1102 92-93 0.2139724818958939 1102 94-95 0.13071755638348748 1102 96-97 0.005954723993806255 1102 98-99 0.1396276364132092 1103 1 0.20568098891290632 1103 2 0.2301915651213129 1103 3 0.17327398603699606 1103 4 0.21071416704518953 1103 5 0.1863651977123979 1103 6 0.3371946043899072 1103 7 0.26609439990323125 1103 8 0.358682607101926 1103 9 0.28144475259922785 1103 10-11 0.2315124432722655 1103 12-13 0.31319515071301396 1103 14-15 0.44608809235978697 1103 16-17 0.24022728571578256 1103 18-19 0.28955157316276114 1103 20-21 0.3390109322691828 1103 22-23 0.23901077283756678 1103 24-25 0.16042133376860335 1103 26-27 0.3036337845415744 1103 28-29 0.27338059410499227 1103 30-31 0.44828997730756015 1103 32-33 0.5092542885127784 1103 34-35 0.5425517463508669 1103 36-37 0.6961616119348903 1103 38-39 0.681356421318057 1103 40-41 0.5304001313456439 1103 42-43 0.3839648357749823 1103 44-45 0.292567560931289 1103 46-47 0.28299629182284747 1103 48-49 0.20175431587079373 1103 50-51 0.20518220720660452 1103 52-53 0.2857550009895391 1103 54-55 0.12848524358217617 1103 56-57 0.2431066904806798 1103 58-59 0.2048221884278263 1103 60-61 0.12537409171702762 1103 62-63 0.23121123741299954 1103 64-65 0.31354768996818194 1103 66-67 0.3253665732292035 1103 68-69 0.4105592051692497 1103 70-71 0.4609287306290497 1103 72-73 0.1746755115531613 1103 74-75 0.2790268978972179 1103 76-77 0.18760861480950553 1103 78-79 0.17527613093484362 1103 80-81 0.08281062823004248 1103 82-83 0.21624920640316603 1103 84-85 0.3269886241318467 1103 86-87 0.19814831256361032 1103 88-89 0.1580112510623728 1103 90-91 0.1634311432575899 1103 92-93 0.12371390623277634 1103 94-95 0.23264758688179654 1103 96-97 0.16151422501459223 1103 98-99 0.1990387280116117 1104 1 0.18263128486300317 1104 2 0.12844159876323147 1104 3 0.0933435385972139 1104 4 0.13357921822393592 1104 5 0.08841208310796844 1104 6 0.16884166631074038 1104 7 0.17592547761826438 1104 8 0.18751101469067777 1104 9 0.19108441718593383 1104 10-11 0.16694041727386377 1104 12-13 0.18483377139616408 1104 14-15 0.21414342931987562 1104 16-17 0.14676782976062697 1104 18-19 0.23994585137797486 1104 20-21 0.19397515230921414 1104 22-23 0.12718174429959817 1104 24-25 0.09282002887641738 1104 26-27 0.20056217356113848 1104 28-29 0.11825091509705032 1104 30-31 0.33253887608046995 1104 32-33 0.4156436829601162 1104 34-35 0.5002866965235313 1104 36-37 0.5803632032503501 1104 38-39 0.5102865116530708 1104 40-41 0.2574305240722907 1104 42-43 0.25571956172384347 1104 44-45 0.20671733858863206 1104 46-47 0.16652188929285217 1104 48-49 -0.009758378209305363 1104 50-51 6.739332064000791E-4 1104 52-53 0.09578751906964555 1104 54-55 -0.046316696181349926 1104 56-57 0.07919904381704157 1104 58-59 0.11576517864671132 1104 60-61 -0.013936824526979308 1104 62-63 0.01922968395880531 1104 64-65 0.07945083642326978 1104 66-67 0.02004672891973769 1104 68-69 0.10722463261480186 1104 70-71 0.14317005294172702 1104 72-73 0.018200331003932035 1104 74-75 0.04220650693442707 1104 76-77 0.04580421228523335 1104 78-79 0.04217732848523781 1104 80-81 0.0562493434866731 1104 82-83 0.1345588211462747 1104 84-85 0.196970272631237 1104 86-87 0.0023318695145206902 1104 88-89 0.02092018367290649 1104 90-91 -0.10298177949832876 1104 92-93 -0.032661097568077224 1104 94-95 -0.01894549593125916 1104 96-97 -0.1331112175336422 1104 98-99 -0.07890142130560562 1105 1 0.07004917610068873 1105 2 0.10614134368202954 1105 3 0.08702967984725163 1105 4 0.04505424439868477 1105 5 -0.03370303078849446 1105 6 0.17407940899354202 1105 7 0.05567948092086539 1105 8 0.1740850046259652 1105 9 0.07465666259321324 1105 10-11 0.1020186324105552 1105 12-13 0.18177165332588885 1105 14-15 0.2238556604064641 1105 16-17 0.04542563220650919 1105 18-19 0.1372329632226652 1105 20-21 0.11905943350731008 1105 22-23 0.05188525181510073 1105 24-25 -0.03392190772471082 1105 26-27 0.1203386277462073 1105 28-29 0.04551117004915284 1105 30-31 0.2872341243054706 1105 32-33 0.4214148284569532 1105 34-35 0.423063501774223 1105 36-37 0.5380721242275612 1105 38-39 0.42275241682513354 1105 40-41 0.27207347835405926 1105 42-43 0.0717764675620387 1105 44-45 0.10097267663577725 1105 46-47 0.08047066809841397 1105 48-49 -0.0663198542937593 1105 50-51 -0.029798482197094245 1105 52-53 0.055417664670017075 1105 54-55 -0.06616057035932954 1105 56-57 0.10962530271875437 1105 58-59 -0.0013050117604578304 1105 60-61 -0.07744775976183149 1105 62-63 -0.03284181735818237 1105 64-65 0.049989395048122276 1105 66-67 0.02770330816014166 1105 68-69 0.12593797896785475 1105 70-71 0.1758050583430304 1105 72-73 -0.04441449774481043 1105 74-75 0.04798679993766797 1105 76-77 0.049895079563810896 1105 78-79 -0.02222612095795995 1105 80-81 -0.08641125600132682 1105 82-83 0.005295493501002113 1105 84-85 0.09488040802488129 1105 86-87 -0.049362784021695916 1105 88-89 -0.1090711582394377 1105 90-91 -0.18422191663985643 1105 92-93 -0.23194022285604632 1105 94-95 -0.13472369222361635 1105 96-97 -0.25470729772706235 1105 98-99 -0.2742696345851705 1106 1 0.16842227929463505 1106 2 0.12192616918488497 1106 3 0.084198780999035 1106 4 -4.7668226618924336E-4 1106 5 0.007645837083359197 1106 6 0.1744512314521529 1106 7 0.13880000790764768 1106 8 0.13329100839558805 1106 9 0.1548997368062004 1106 10-11 0.1310260820129585 1106 12-13 0.08012465825543558 1106 14-15 0.21562182682801279 1106 16-17 0.05039884893236035 1106 18-19 0.3128126920419376 1106 20-21 0.13282292348917935 1106 22-23 0.03500156793995046 1106 24-25 0.14207711089783714 1106 26-27 0.2513827978915799 1106 28-29 0.184234469591658 1106 30-31 0.35104247534289357 1106 32-33 0.39675179788834924 1106 34-35 0.4391316546508648 1106 36-37 0.5610418280654343 1106 38-39 0.5269633944660939 1106 40-41 0.3312418279446234 1106 42-43 0.2350446316919914 1106 44-45 0.13718794661566136 1106 46-47 0.1250889673169695 1106 48-49 0.00874297675975555 1106 50-51 0.024942783933283863 1106 52-53 0.06149170361918266 1106 54-55 -0.060105104264721376 1106 56-57 -0.05161784176660689 1106 58-59 -0.024180831340949283 1106 60-61 0.01938271562529792 1106 62-63 0.04943358385201435 1106 64-65 0.09446160810909277 1106 66-67 0.10868110623071203 1106 68-69 0.13507603653712863 1106 70-71 0.16778357947613642 1106 72-73 0.01175824831445027 1106 74-75 0.124820628790701 1106 76-77 0.13098748128113868 1106 78-79 0.10562835932607584 1106 80-81 0.106204408429452 1106 82-83 0.07584789160362249 1106 84-85 0.1403290965255941 1106 86-87 0.009379211991408454 1106 88-89 -0.0736448387629558 1106 90-91 -0.11815408650462444 1106 92-93 -0.10175944686879035 1106 94-95 0.022576267780806347 1106 96-97 -0.1435888471245228 1106 98-99 -0.09156586114135834 1107 1 0.009481112502147226 1107 2 0.06724581602363244 1107 3 -0.03270687348197043 1107 4 -0.0966745014712771 1107 5 -0.09005385161520252 1107 6 -0.0044484922385166215 1107 7 -0.007087487192947606 1107 8 -0.012209031267332193 1107 9 -0.09897714098410404 1107 10-11 -0.021726770284438146 1107 12-13 0.004913176659293583 1107 14-15 0.1415076259866339 1107 16-17 -0.08166554235997836 1107 18-19 0.12187419366740215 1107 20-21 0.04069463053017586 1107 22-23 -0.060084962575288614 1107 24-25 -0.011042893200965409 1107 26-27 -0.014639386840244129 1107 28-29 -0.031802223733407686 1107 30-31 0.16263245047607455 1107 32-33 0.23828395540055425 1107 34-35 0.22728784584039374 1107 36-37 0.41604458258459687 1107 38-39 0.36252540601336847 1107 40-41 0.1686513429795724 1107 42-43 0.03964391271092893 1107 44-45 0.037060770454090175 1107 46-47 -0.08716801187250667 1107 48-49 -0.22193918884392616 1107 50-51 -0.13762875396921004 1107 52-53 -0.0732035187890574 1107 54-55 -0.12022103840256904 1107 56-57 -0.0047591852944250945 1107 58-59 -0.1327287551476175 1107 60-61 -0.11767050919173272 1107 62-63 -0.04588112835619995 1107 64-65 0.02092276690064665 1107 66-67 0.08633183925718413 1107 68-69 0.08268465042338846 1107 70-71 0.09494212957496018 1107 72-73 -0.2345908670825736 1107 74-75 -0.02888385621285039 1107 76-77 0.007249499549821792 1107 78-79 0.01603465569878182 1107 80-81 -0.007073138396023637 1107 82-83 0.06468045695135416 1107 84-85 0.0816267871364893 1107 86-87 -0.16775506218349534 1107 88-89 -0.23784201664595273 1107 90-91 -0.11161272557669477 1107 92-93 -0.19529653787230927 1107 94-95 -0.08022064543091645 1107 96-97 -0.23045394140084952 1107 98-99 -0.2107463173422559 1108 1 0.0497630726370879 1108 2 0.036185021066753364 1108 3 -0.0010077140736051149 1108 4 -0.030484881033956412 1108 5 -0.030981430948564537 1108 6 0.07483781115446675 1108 7 0.0665957007612974 1108 8 0.0035058427931033975 1108 9 0.06184443735046585 1108 10-11 -0.012789556966815496 1108 12-13 -0.025508343672818512 1108 14-15 -0.03820275599133538 1108 16-17 -0.07020117746379384 1108 18-19 0.18245115294633507 1108 20-21 0.06625060876131528 1108 22-23 0.038278394932248716 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2216 1 -0.1686372277385786 2216 2 -0.1765956847762311 2216 3 -0.12080126887755682 2216 4 -0.14222999129785308 2216 5 -0.24434185718455836 2216 6 -0.20211532785094732 2216 7 -0.3522826178785934 2216 8 -0.3392483411531302 2216 9 -0.22778984664131485 2216 10-11 -0.32225861940165856 2216 12-13 -0.36859405132842227 2216 14-15 -0.3815001425105464 2216 16-17 -0.43629763496965523 2216 18-19 -0.35136219053230633 2216 20-21 -0.179277286205469 2216 22-23 -0.20336085017137862 2216 24-25 -0.2820373077972036 2216 26-27 -0.3850115095192592 2216 28-29 -0.31391700023787905 2216 30-31 -0.1840475563887054 2216 32-33 -0.08180397159483022 2216 34-35 -0.2608022924895934 2216 36-37 -0.08252466320375618 2216 38-39 -8.435797761330335 2216 40-41 -1.8020737067307593 2216 42-43 -0.48544493003398514 2216 44-45 -0.5140297819684818 2216 46-47 -0.22045940955862875 2216 48-49 -0.13995884203219333 2216 50-51 -0.11731206505216818 2216 52-53 -0.1273703546521503 2216 54-55 -0.10059377269533343 2216 56-57 -0.05533347973697289 2216 58-59 -0.029435862684181302 2216 60-61 -0.10005768358470135 2216 62-63 -0.17639191857619352 2216 64-65 -0.18865217575221038 2216 66-67 -0.11205366339154921 2216 68-69 -0.07210001976223168 2216 70-71 0.10822737018229844 2216 72-73 0.057433409805327074 2216 74-75 -0.017416202976448858 2216 76-77 -0.09611096704127675 2216 78-79 -0.08770443489174085 2216 80-81 -0.0636679220660703 2216 82-83 -0.15147727104730535 2216 84-85 -0.006757477245081134 2216 86-87 0.05503405857830046 2216 88-89 0.05959873912147273 2216 90-91 0.05186460937156667 2216 92-93 0.06924704370100443 2216 94-95 0.15915899942871548 2216 96-97 0.2430581076405467 2216 98-99 0.2750088476606045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 220.0 8 1349.0 9 2504.0 10 3875.0 11 6995.0 12 13140.0 13 21372.0 14 28739.0 15 32522.0 16 34755.0 17 38129.0 18 41836.0 19 45774.0 20 50715.0 21 56876.0 22 63817.0 23 72179.0 24 81220.0 25 90922.0 26 99959.0 27 111142.0 28 131750.0 29 162344.0 30 203791.0 31 261677.0 32 346524.0 33 466091.0 34 633672.0 35 901876.0 36 1351065.0 37 1594502.0 38 468985.0 39 316.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.76344561301754 18.61525076826299 12.4369932711967 25.18431034752277 2 16.0707410430575 20.639102116536236 37.846320578216506 25.443836262189755 3 18.006160550589776 25.998987077830048 29.02884537115053 26.96600700042964 4 13.07235660124011 17.324930100331024 36.476523036569915 33.12619026185896 5 14.462040080999705 37.451838031857044 33.25644039559725 14.829681491545998 6 31.678187691728848 36.926295805373016 17.47653435635436 13.918982146543774 7 27.82807179918136 31.98170602699032 21.920169107938374 18.270053065889943 8 26.689410449454737 33.727917660965986 19.78597252310988 19.796699366469397 9 26.704193548908744 15.779779522941158 19.225602765046016 38.29042416310408 10-11 25.336684438954048 25.749688150024348 26.884435975867564 22.02919143515404 12-13 26.077785848371366 23.72560685548262 26.387348513535912 23.8092587826101 14-15 24.09583293269417 24.58784590039842 23.705640148161137 27.610681018746273 16-17 24.071015283342597 27.003698339278024 24.46031521860414 24.464971158775242 18-19 24.428350885288 26.57235848787085 25.654195947624974 23.345094679216174 20-21 25.85001474267165 25.762454862359508 24.61643449429402 23.771095900674823 22-23 25.31852237663029 25.507569604073506 24.54015206924747 24.633755950048734 24-25 24.660401418356088 26.002799652991264 24.67013787701404 24.666661051638606 26-27 24.78731719412865 25.993943755356824 24.546260060050347 24.672478990464178 28-29 24.45620109313654 25.98778623814464 24.946991314041327 24.6090213546775 30-31 24.796118827225484 25.92459002585764 24.818374927354352 24.460916219562527 32-33 24.502767307632702 26.132392794623595 24.39622562687233 24.968614270871374 34-35 24.86337927033059 26.11404725566257 24.842114242387225 24.180459231619615 36-37 24.815384423611363 25.939953150830675 24.643842211350865 24.600820214207097 38-39 24.709110668100738 25.78173168543183 24.830325371709247 24.678832274758182 40-41 24.672548344198077 25.940827175926902 24.961726779879932 24.424897699995093 42-43 24.922440711943832 25.860745880377678 24.8418072171526 24.37500619052589 44-45 24.473650236987773 26.155413093971998 24.705550844968005 24.66538582407222 46-47 24.29441586810396 26.074710013912 24.97666116417044 24.654212953813598 48-49 24.813791016999573 26.0622568643959 24.78770157914543 24.336250539459094 50-51 24.22223755495189 26.275465994446257 24.746610171547193 24.75568627905466 52-53 24.323647794199296 26.01759072292651 24.688321023483763 24.970440459390435 54-55 24.4768507712816 25.888778266445083 24.797692542795524 24.836678419477792 56-57 24.609964364086807 25.67844881629582 24.588739676716344 25.122847142901033 58-59 24.29546633842883 25.721920986416862 25.128818145022752 24.85379453013155 60-61 24.401106833769358 25.911579029075238 24.889987388531143 24.797326748624258 62-63 24.13920915770994 26.010154794756378 24.665761308082566 25.184874739451118 64-65 24.06610111715804 25.85109864531305 24.832814404229435 25.249985833299476 66-67 24.634654899348803 25.829262713254842 24.661391452994906 24.874690934401446 68-69 24.482333055231866 25.666887629533807 24.792714235718012 25.05806507951631 70-71 24.12441420083896 25.777858274066144 24.79998334373676 25.29774418135814 72-73 24.61025029379761 25.8262466024019 24.839136502872886 24.724366600927606 74-75 24.744783979306053 25.814431655970548 24.778575377559076 24.662208987164323 76-77 24.492825807418402 25.9015748806098 24.801034306270118 24.804565005701676 78-79 24.58744731577974 25.891615911753018 24.806539284815248 24.714397487651986 80-81 24.365384584286307 25.76820908612146 25.09418330180863 24.772223027783603 82-83 24.66408255762784 25.62980498706946 24.979777607246735 24.726334848055963 84-85 24.690643047534802 25.76086727283104 24.661878505825914 24.886611173808245 86-87 24.4634422170822 25.595845153973322 24.848335102274252 25.092377526670223 88-89 24.61777534971396 25.19864874115911 24.978862997726882 25.204712911400044 90-91 24.777173932085685 25.36755183485509 24.847222308319047 25.008051924740176 92-93 24.65498914109249 25.43083234375059 24.82685446368748 25.087324051469444 94-95 24.15697252903929 25.482128486021953 25.20295430203018 25.157944682908578 96-97 24.5973203112387 25.503354664578016 24.788414770388822 25.110910253794465 98-99 24.397301183646462 25.657009362167788 24.714175591520007 25.231513862665743 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1114.0 1 1049.0 2 998.5 3 1010.0 4 956.5 5 862.5 6 771.0 7 694.5 8 665.5 9 666.0 10 639.5 11 618.5 12 609.0 13 611.5 14 651.5 15 832.0 16 1011.0 17 1133.0 18 1431.5 19 1899.5 20 2611.5 21 3663.0 22 5315.5 23 7636.5 24 10308.0 25 14002.5 26 19125.0 27 24742.0 28 32234.5 29 41583.0 30 49985.5 31 59814.0 32 70446.0 33 81094.5 34 91101.0 35 101689.5 36 111293.5 37 123609.0 38 140386.0 39 145902.5 40 150708.0 41 162792.0 42 176643.0 43 198082.5 44 222612.5 45 249705.0 46 281270.0 47 306960.5 48 323420.5 49 333692.5 50 344277.0 51 341153.5 52 333242.0 53 332831.0 54 318573.5 55 303730.5 56 281432.5 57 255289.5 58 239252.5 59 213621.0 60 181558.0 61 146261.5 62 118812.5 63 96464.0 64 73521.0 65 56792.0 66 42533.0 67 33022.0 68 26463.0 69 22364.5 70 19515.0 71 16669.5 72 15087.5 73 13116.5 74 9926.0 75 7519.5 76 5550.5 77 3844.5 78 2573.0 79 1736.5 80 1166.5 81 804.0 82 534.5 83 356.5 84 256.5 85 174.5 86 128.0 87 94.0 88 71.0 89 60.5 90 43.0 91 27.5 92 21.0 93 15.5 94 9.5 95 9.0 96 9.5 97 8.5 98 6.5 99 6.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0021965772206048104 3 0.007007485611745409 4 0.0 5 0.004878288060484303 6 0.0 7 6.737966934370585E-4 8 0.0 9 0.0 10-11 6.737966934370586E-6 12-13 8.085560321244702E-5 14-15 0.002715400674551346 16-17 1.0780747094992937E-4 18-19 0.0 20-21 0.0 22-23 4.7165768540594093E-5 24-25 9.90481139352476E-4 26-27 0.002641283038273269 28-29 0.0020752938157861402 30-31 0.002674972872945122 32-33 8.894116353369171E-4 34-35 2.0213900803111754E-5 36-37 1.819251072280058E-4 38-39 0.06005549928604502 40-41 0.0040966838960973155 42-43 3.0320851204667634E-4 44-45 0.0 46-47 8.691977345338055E-4 48-49 1.1454543788429994E-4 50-51 7.277004289120232E-4 52-53 2.695186773748234E-4 54-55 0.0 56-57 4.312298837997175E-4 58-59 6.737966934370586E-6 60-61 1.280213717530411E-4 62-63 4.7165768540594093E-5 64-65 8.826736684025466E-4 66-67 0.0011252404780398876 68-69 0.0011185025111055172 70-71 5.3903735474964685E-5 72-73 0.0018394649730831699 74-75 0.001462138824758417 76-77 1.347593386874117E-4 78-79 1.0780747094992937E-4 80-81 0.0 82-83 3.4363631365289985E-4 84-85 5.592512555527586E-4 86-87 0.0 88-89 0.0 90-91 6.737966934370585E-4 92-93 0.0 94-95 0.0 96-97 0.00299165731886054 98-99 0.004662673118584444 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7420636.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.60831149416028 #Duplication Level Percentage of deduplicated Percentage of total 1 74.5790312394457 33.26844656461905 2 13.75772075602662 12.274173858690196 3 4.8244022560761834 6.456253158365281 4 2.1413045693043835 3.820799249255947 5 1.1764769807473223 2.624032581144288 6 0.727493091746952 1.9471343067898657 7 0.48555371138320097 1.5161811843169202 8 0.3544591223187824 1.2649458352274328 9 0.25496352542268313 1.0236143125533872 >10 1.4534740923340956 12.007985898538752 >50 0.12134630759086835 3.7578413496655334 >100 0.10134058118737807 9.487498381777595 >500 0.015067605417097162 4.650544976253935 >1k 0.007274844953554124 5.5763013572812365 >5k 9.131604527335175E-5 0.324246985520694 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 9631 0.1297867190898462 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9138 0.1231430836925568 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.347593386874117E-5 2 0.0 0.0 0.0 0.0 1.347593386874117E-5 3 0.0 0.0 0.0 1.347593386874117E-5 1.347593386874117E-5 4 0.0 1.347593386874117E-5 0.0 2.695186773748234E-5 1.347593386874117E-5 5 0.0 2.695186773748234E-5 0.0 4.042780160622351E-5 1.347593386874117E-5 6 0.0 2.695186773748234E-5 0.0 4.042780160622351E-5 2.695186773748234E-5 7 0.0 2.695186773748234E-5 0.0 4.042780160622351E-5 2.695186773748234E-5 8 1.347593386874117E-5 2.695186773748234E-5 0.0 4.042780160622351E-5 2.695186773748234E-5 9 1.347593386874117E-5 2.695186773748234E-5 0.0 4.042780160622351E-5 2.695186773748234E-5 10-11 1.347593386874117E-5 2.695186773748234E-5 0.0 1.1454543788429994E-4 2.695186773748234E-5 12-13 1.347593386874117E-5 2.695186773748234E-5 0.0 1.347593386874117E-4 8.085560321244702E-5 14-15 1.347593386874117E-5 2.695186773748234E-5 0.0 1.4149730562178228E-4 9.433153708118819E-5 16-17 1.347593386874117E-5 2.695186773748234E-5 0.0 1.5497323949052345E-4 1.1454543788429994E-4 18-19 2.695186773748234E-5 4.7165768540594093E-5 0.0 1.8866307416237637E-4 1.2128340481867053E-4 20-21 5.390373547496468E-5 6.737966934370585E-5 0.0 3.0320851204667634E-4 1.2128340481867053E-4 22-23 6.737966934370585E-5 6.737966934370585E-5 0.0 9.500533377462525E-4 1.280213717530411E-4 24-25 8.75935701468176E-5 6.737966934370585E-5 0.0 0.002621069137470158 1.4823527255615287E-4 26-27 1.347593386874117E-4 6.737966934370585E-5 0.0 0.004393154441209622 1.4823527255615287E-4 28-29 1.5497323949052345E-4 6.737966934370585E-5 0.0 0.01105700373930213 1.5497323949052345E-4 30-31 1.8866307416237637E-4 6.737966934370585E-5 0.0 0.03278694710264727 1.6171120642489403E-4 32-33 2.2909087576859988E-4 6.737966934370585E-5 0.0 0.0738279036998985 1.6171120642489403E-4 34-35 2.3582884270297047E-4 7.411763627807643E-5 0.0 0.12877602404969063 1.8866307416237637E-4 36-37 2.4256680963734105E-4 8.085560321244702E-5 0.0 0.21471205433065305 2.0213900803111754E-4 38-39 2.560427435060822E-4 8.085560321244702E-5 0.0 0.3461832651540919 2.0887697496548813E-4 40-41 2.695186773748234E-4 9.433153708118819E-5 0.0 0.49992345669562555 2.156149418998587E-4 42-43 3.3689834671852924E-4 1.0106950401555877E-4 0.0 0.6457195852215363 2.223529088342293E-4 44-45 3.638502144560116E-4 1.2128340481867053E-4 0.0 0.8175377420479861 2.4256680963734105E-4 46-47 3.7058818139038214E-4 1.347593386874117E-4 0.0 1.007117179713437 2.4256680963734105E-4 48-49 3.8406411525912336E-4 1.347593386874117E-4 0.0 1.2072347976642432 2.4930477657171166E-4 50-51 3.908020821934939E-4 1.4823527255615287E-4 0.0 1.429850487208913 2.560427435060822E-4 52-53 3.908020821934939E-4 1.4823527255615287E-4 0.0 1.6479382629736858 2.695186773748234E-4 54-55 4.042780160622351E-4 1.6171120642489403E-4 0.0 1.8664842204899958 2.8299461124356456E-4 56-57 4.042780160622351E-4 1.6171120642489403E-4 0.0 2.115020868831189 2.8299461124356456E-4 58-59 4.1775394993097626E-4 1.6171120642489403E-4 0.0 2.390260080133293 2.8299461124356456E-4 60-61 4.1775394993097626E-4 1.6171120642489403E-4 0.0 2.6665436763102246 2.8299461124356456E-4 62-63 4.1775394993097626E-4 1.6171120642489403E-4 0.0 2.9448621385013363 3.099464789810469E-4 64-65 4.1775394993097626E-4 1.8866307416237637E-4 0.0 3.2361040212725705 3.099464789810469E-4 66-67 4.1775394993097626E-4 1.8866307416237637E-4 0.0 3.556851461249413 3.301603797841587E-4 68-69 4.1775394993097626E-4 1.8866307416237637E-4 0.0 3.9030616782712424 3.503742805872704E-4 70-71 4.1775394993097626E-4 1.9540104109674696E-4 0.0 4.279363655621971 3.503742805872704E-4 72-73 4.1775394993097626E-4 2.0213900803111754E-4 0.0 4.656305739831465 3.908020821934939E-4 74-75 4.1775394993097626E-4 2.0213900803111754E-4 0.0 5.032439268008834 3.908020821934939E-4 76-77 4.1775394993097626E-4 2.0213900803111754E-4 0.0 5.438307444267581 3.908020821934939E-4 78-79 4.1775394993097626E-4 2.0213900803111754E-4 0.0 5.8607240673171415 3.908020821934939E-4 80-81 4.1775394993097626E-4 2.2909087576859988E-4 0.0 6.32271277017226 4.110159829966057E-4 82-83 4.1775394993097626E-4 2.3582884270297047E-4 0.0 6.811842003839024 4.5818175153719976E-4 84-85 4.1775394993097626E-4 2.4256680963734105E-4 0.0 7.29608621147837 4.7165768540594093E-4 86-87 4.1775394993097626E-4 2.560427435060822E-4 0.0 7.796124213611879 4.783956523403115E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 2495 0.0 24.414017 1 GTATCAA 24795 0.0 23.10389 1 GTATAAG 3020 0.0 20.169859 1 GTACATG 39905 0.0 19.692368 1 TAGTACT 3425 0.0 19.412775 4 CAACGCA 27625 0.0 19.153675 5 TACATGG 39500 0.0 18.893675 2 ATCAACG 28175 0.0 18.863436 3 AACGCAG 28985 0.0 18.63946 6 CTAGTAC 3665 0.0 18.396387 3 CATGGGG 17295 0.0 18.38856 4 TATCAAC 31045 0.0 18.362732 2 TCAACGC 30020 0.0 17.795963 4 ACATGGG 39230 0.0 17.447292 3 ACGCAGA 30165 0.0 17.340023 7 TAGGCAT 4140 0.0 17.183168 5 CGCAGAG 30480 0.0 16.947264 8 GTATTAG 2925 0.0 16.532784 1 CTAGGCA 4415 0.0 16.428808 4 TATTCGC 510 0.0 16.410112 9 >>END_MODULE