FastQCFastQC Report
Fri 27 May 2016
ERR765435_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765435_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7420636
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192860.2598968605925422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123420.1663199758080035No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT105110.14164554089433845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101100.1362416914129732No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA92730.12496233476483687No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA344300.034.7856831
CAACGCA436300.027.316565
ATCAACG438300.027.1494753
AACGCAG462000.026.228926
TCAACGC459650.025.9491234
TATCAAC462050.025.945872
GGTATCA216950.025.4577541
ACGCAGA491400.023.9594337
CGCAGAG498950.023.5605588
AGTACTT284750.022.38288112-13
GCAGAGT534000.021.8927979
AGAGTAC504950.020.21560710-11
TAGTACT32350.019.9803524
GAGTACT273900.019.55116312-13
TACTTTT331950.019.5010714-15
GTACTAG24850.019.2858941
CGTTAAC11150.018.3614431
GTATAAG26500.018.260661
GTACTTT306700.017.7176814-15
CTAGTAC36150.017.1082653
Produced by FastQC (version 0.11.5)