##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765435_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7420636 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.309845139958355 33.0 33.0 33.0 33.0 33.0 2 32.16298441265681 33.0 33.0 33.0 33.0 33.0 3 32.21689906363821 33.0 33.0 33.0 33.0 33.0 4 36.158896892395745 37.0 37.0 37.0 33.0 37.0 5 36.083202976133045 37.0 37.0 37.0 33.0 37.0 6 36.07556441253823 37.0 37.0 37.0 33.0 37.0 7 35.999924804289016 37.0 37.0 37.0 33.0 37.0 8 35.96064151374626 37.0 37.0 37.0 33.0 37.0 9 35.712406187286376 37.0 37.0 37.0 33.0 37.0 10-11 35.8860014155121 37.0 37.0 37.0 33.0 37.0 12-13 35.813337428220436 37.0 37.0 37.0 33.0 37.0 14-15 37.215286129113466 37.0 37.0 40.0 33.0 40.0 16-17 37.35726452557435 37.0 37.0 40.0 33.0 40.0 18-19 37.420137775252684 37.0 37.0 40.0 33.0 40.0 20-21 37.44278967463166 37.0 37.0 40.0 33.0 40.0 22-23 37.363882624076965 37.0 37.0 40.0 33.0 40.0 24-25 37.31303886890558 37.0 37.0 40.0 33.0 40.0 26-27 37.18614213121355 37.0 37.0 40.0 33.0 40.0 28-29 37.13259638392181 37.0 37.0 40.0 33.0 40.0 30-31 37.038889860653455 37.0 37.0 40.0 33.0 40.0 32-33 36.88581423479066 37.0 37.0 40.0 33.0 40.0 34-35 36.80654367091985 37.0 37.0 40.0 33.0 40.0 36-37 36.661936119761165 37.0 37.0 40.0 33.0 40.0 38-39 36.52140955303562 37.0 37.0 40.0 33.0 40.0 40-41 36.402530120059794 37.0 37.0 40.0 33.0 40.0 42-43 36.238589589894985 37.0 37.0 40.0 33.0 40.0 44-45 36.04612946922609 37.0 37.0 40.0 33.0 40.0 46-47 35.81770598908234 37.0 37.0 40.0 30.0 40.0 48-49 35.58905281164579 37.0 37.0 40.0 27.0 40.0 50-51 35.29069671386657 37.0 33.0 40.0 27.0 40.0 52-53 35.13839238307875 37.0 33.0 40.0 27.0 40.0 54-55 35.01279607300506 37.0 33.0 40.0 27.0 40.0 56-57 34.83697657451464 37.0 33.0 40.0 27.0 40.0 58-59 34.64385054057361 37.0 33.0 38.5 27.0 40.0 60-61 34.47314131834521 37.0 33.0 37.0 27.0 40.0 62-63 34.161228431094045 37.0 33.0 37.0 27.0 40.0 64-65 34.00807390363845 37.0 33.0 37.0 27.0 40.0 66-67 33.81634653687366 37.0 33.0 37.0 27.0 40.0 68-69 33.57059031058793 37.0 33.0 37.0 27.0 40.0 70-71 33.371788415440406 37.0 33.0 37.0 27.0 37.0 72-73 33.17190561563726 37.0 33.0 37.0 27.0 37.0 74-75 32.96764738224594 37.0 33.0 37.0 27.0 37.0 76-77 32.80985827899387 35.0 33.0 37.0 27.0 37.0 78-79 32.62092103695694 33.0 33.0 37.0 27.0 37.0 80-81 32.47858431541448 33.0 33.0 37.0 22.0 37.0 82-83 32.342812260296824 33.0 33.0 37.0 22.0 37.0 84-85 32.19657048263788 33.0 33.0 37.0 22.0 37.0 86-87 32.1026907936193 33.0 33.0 37.0 22.0 37.0 88-89 32.002533610326665 33.0 33.0 37.0 22.0 37.0 90-91 31.950740879892237 33.0 33.0 37.0 22.0 37.0 92-93 31.857512213238863 33.0 33.0 37.0 22.0 37.0 94-95 31.792405192762452 33.0 33.0 37.0 22.0 37.0 96-97 31.74235752838436 33.0 33.0 37.0 22.0 37.0 98-99 30.923745889166376 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18655842794132838 1101 2 0.3443779389633903 1101 3 0.25528741148964684 1101 4 0.18042470579321446 1101 5 0.1282765957155263 1101 6 0.1957336247694883 1101 7 0.17053128435595966 1101 8 0.14614930250294123 1101 9 0.42647000715452776 1101 10-11 0.3482670383385411 1101 12-13 0.30131673908639556 1101 14-15 0.5392549895354435 1101 16-17 0.45253639213326124 1101 18-19 0.2964837313057771 1101 20-21 0.4153034896129242 1101 22-23 0.2786302247475021 1101 24-25 0.3880586348107755 1101 26-27 0.21479806761643516 1101 28-29 0.25639927727218037 1101 30-31 0.2762103852472535 1101 32-33 0.30645163164798817 1101 34-35 0.3730347657331521 1101 36-37 0.4438282215494951 1101 38-39 0.2505676507205621 1101 40-41 0.15694915600629855 1101 42-43 0.3033522631324743 1101 44-45 0.40735104815214385 1101 46-47 0.4663574689091803 1101 48-49 0.5028948667012543 1101 50-51 0.4420598824036759 1101 52-53 0.47179028533216893 1101 54-55 0.45564305920277803 1101 56-57 0.5043808216853165 1101 58-59 0.4948568301101801 1101 60-61 0.40467762333774004 1101 62-63 0.4136385975436099 1101 64-65 0.3362458014906835 1101 66-67 0.3325009718582379 1101 68-69 0.2937450470035827 1101 70-71 0.36167492630394804 1101 72-73 0.42871370395214115 1101 74-75 0.249547616370414 1101 76-77 0.2735380177083897 1101 78-79 0.5764451396934334 1101 80-81 0.5418329244481299 1101 82-83 0.4704581387377118 1101 84-85 0.49053026603017 1101 86-87 0.33909354769803457 1101 88-89 0.4388146892358691 1101 90-91 0.3275365024014647 1101 92-93 0.48506147232788166 1101 94-95 0.5307820167336921 1101 96-97 0.3756668844662663 1101 98-99 0.4091857248790127 1102 1 0.11572645902831624 1102 2 0.3075891778208373 1102 3 0.26964283224029373 1102 4 0.1673951282408055 1102 5 0.08944010471086017 1102 6 0.2360466439369091 1102 7 0.17744783476178583 1102 8 0.07657097937443069 1102 9 0.38209038655285354 1102 10-11 0.2910080917577389 1102 12-13 0.27585490446113425 1102 14-15 0.5614565650167691 1102 16-17 0.3736836277249864 1102 18-19 0.24118396758829164 1102 20-21 0.3602756212442273 1102 22-23 0.24431742377070265 1102 24-25 0.33921948994083095 1102 26-27 0.2411096273925395 1102 28-29 0.24700419418562802 1102 30-31 0.24460346842806047 1102 32-33 0.337131818838337 1102 34-35 0.3366518695207361 1102 36-37 0.33480824215776295 1102 38-39 0.19684047693010598 1102 40-41 0.23913947428938087 1102 42-43 0.18633759509747705 1102 44-45 0.3139438250851825 1102 46-47 0.35354976540457983 1102 48-49 0.3824415811356374 1102 50-51 0.3091658627916374 1102 52-53 0.33252693735718 1102 54-55 0.33837044385993664 1102 56-57 0.3974564360430435 1102 58-59 0.3039972214920468 1102 60-61 0.22672796038610699 1102 62-63 0.30437486540109404 1102 64-65 0.1733640544847148 1102 66-67 0.2657577908300084 1102 68-69 0.24971840169677506 1102 70-71 0.23532070890185253 1102 72-73 0.29820127256980555 1102 74-75 0.20200815615784506 1102 76-77 0.24826860879272772 1102 78-79 0.3643881230920769 1102 80-81 0.359805296464458 1102 82-83 0.27799486325754685 1102 84-85 0.39516543064610943 1102 86-87 0.26330357904796386 1102 88-89 0.2885074271740393 1102 90-91 0.2192443578689378 1102 92-93 0.29521494952478733 1102 94-95 0.34145460916007764 1102 96-97 0.23139942631216215 1102 98-99 0.19488779725332606 1103 1 0.08180826404019825 1103 2 0.2546542120432136 1103 3 0.22000557677382915 1103 4 0.18923514265379993 1103 5 0.04409147695555049 1103 6 0.11912732844662344 1103 7 0.14544046900191887 1103 8 0.020860947181716938 1103 9 0.3580164731613422 1103 10-11 0.2640955478017375 1103 12-13 0.24589406586628826 1103 14-15 0.4457558984541592 1103 16-17 0.38043276972899065 1103 18-19 0.1920590620503546 1103 20-21 0.29046429378634997 1103 22-23 0.16482094113099777 1103 24-25 0.2770974041738441 1103 26-27 0.1239995394387634 1103 28-29 0.018933561677215494 1103 30-31 0.0786483044218329 1103 32-33 0.0746651760140935 1103 34-35 0.2294363287711434 1103 36-37 0.20746738273525978 1103 38-39 0.06683219566725995 1103 40-41 0.012631564473529977 1103 42-43 -0.027692065675758215 1103 44-45 0.17257723284021154 1103 46-47 0.2705286330774328 1103 48-49 0.2144749966745465 1103 50-51 0.20106198024793542 1103 52-53 0.22100290910051257 1103 54-55 0.18863189543274217 1103 56-57 0.25728405205425275 1103 58-59 0.23199775180140847 1103 60-61 0.13939244342473245 1103 62-63 0.20869014460630098 1103 64-65 0.04198526726884921 1103 66-67 0.1048892670638466 1103 68-69 0.12675099019760694 1103 70-71 0.09933743360254255 1103 72-73 0.09403560516079779 1103 74-75 0.12488616736939662 1103 76-77 -0.0027181718165678603 1103 78-79 0.213176873178611 1103 80-81 0.14561922179488818 1103 82-83 0.15562404978974342 1103 84-85 0.20271728687770718 1103 86-87 0.12811666796253007 1103 88-89 0.13865894973545778 1103 90-91 -0.004976162744490864 1103 92-93 -0.053270454581408444 1103 94-95 0.15587138952581725 1103 96-97 0.12945106831080366 1103 98-99 0.10506604108213935 1104 1 0.1200522915394302 1104 2 0.2764023195696126 1104 3 0.2017357825272441 1104 4 0.1644172217825286 1104 5 0.03896172942071274 1104 6 0.1417931703682882 1104 7 0.14915102468614094 1104 8 0.01463964600336709 1104 9 0.35381408565906725 1104 10-11 0.26675037506202415 1104 12-13 0.25678062977066674 1104 14-15 0.3935733585060177 1104 16-17 0.29549907060868463 1104 18-19 0.2389717647871521 1104 20-21 0.30622732666019203 1104 22-23 0.21755094716837675 1104 24-25 0.27168166843698316 1104 26-27 0.18485291401827197 1104 28-29 0.1645022497468389 1104 30-31 0.20151378747934245 1104 32-33 0.2738150457843176 1104 34-35 0.32234997603156756 1104 36-37 0.1829381831732917 1104 38-39 0.025721449395426532 1104 40-41 0.04612173832337163 1104 42-43 0.13716418435571853 1104 44-45 0.13434065564798203 1104 46-47 0.2441073742646438 1104 48-49 0.3128341888681163 1104 50-51 0.2506165698187388 1104 52-53 0.24582337980947955 1104 54-55 0.2106492817560408 1104 56-57 0.2502186908534867 1104 58-59 0.22981501902178536 1104 60-61 0.24469653292454296 1104 62-63 0.2607773206247188 1104 64-65 0.1879159287037524 1104 66-67 0.18593804144292392 1104 68-69 0.126335486177247 1104 70-71 0.1661554283553599 1104 72-73 0.21402150870184045 1104 74-75 0.13361901086616257 1104 76-77 0.15133683825433764 1104 78-79 0.26407967349323513 1104 80-81 0.20648520213448762 1104 82-83 0.14139377216277893 1104 84-85 0.19117398040244638 1104 86-87 0.15478591442748524 1104 88-89 0.13007558250522067 1104 90-91 0.1132522412617405 1104 92-93 0.05746779323260043 1104 94-95 0.18178716692064967 1104 96-97 0.1402741890660799 1104 98-99 0.07640082151211303 1105 1 0.09799925965080547 1105 2 0.2729945436895136 1105 3 0.20946189523023406 1105 4 0.15247060945407043 1105 5 -0.06954842777177106 1105 6 0.06829670386612463 1105 7 0.1164333813792382 1105 8 -0.026661787655285707 1105 9 0.34782345431943185 1105 10-11 0.23310080335463823 1105 12-13 0.20195625661770578 1105 14-15 0.3879167546860529 1105 16-17 0.319217980569789 1105 18-19 0.18532828893457776 1105 20-21 0.2931388820820118 1105 22-23 0.09424169963061502 1105 24-25 0.2567164643409825 1105 26-27 0.08544922746922623 1105 28-29 0.13835368760139488 1105 30-31 0.13466405895933065 1105 32-33 0.13764432665593773 1105 34-35 0.24727077367798245 1105 36-37 0.14339910701216496 1105 38-39 0.08642797175524919 1105 40-41 0.061536116794464135 1105 42-43 0.1893019061707193 1105 44-45 0.1719548373212163 1105 46-47 0.3260841921402857 1105 48-49 0.33064400365989854 1105 50-51 0.273518209436709 1105 52-53 0.2339631140448617 1105 54-55 0.2414852620472132 1105 56-57 0.33667555236157654 1105 58-59 0.3175825886272534 1105 60-61 0.22964659607415427 1105 62-63 0.23072420860424359 1105 64-65 0.08590753043942101 1105 66-67 0.16225977154844173 1105 68-69 0.12659952247108208 1105 70-71 0.23431561669437428 1105 72-73 0.24081174630921964 1105 74-75 0.15416806545934492 1105 76-77 0.23976819561455898 1105 78-79 0.3146614967974912 1105 80-81 0.26371924106255307 1105 82-83 0.18950973343334 1105 84-85 0.1568953876715966 1105 86-87 0.1991761017708953 1105 88-89 0.10813906300307963 1105 90-91 0.12002538397189966 1105 92-93 0.08056047530196153 1105 94-95 0.30429167441456073 1105 96-97 0.17641436529904198 1105 98-99 0.1258049984109526 1106 1 0.10786951395154887 1106 2 0.2551394538504823 1106 3 0.21581454222128826 1106 4 0.1794472544243959 1106 5 0.03961166755507861 1106 6 0.11778736312086835 1106 7 0.10736809286303384 1106 8 -0.014223242490729149 1106 9 0.34100158394894464 1106 10-11 0.18621609827695096 1106 12-13 0.2117806725044744 1106 14-15 0.367276619636435 1106 16-17 0.2675376578819737 1106 18-19 0.1464401720539641 1106 20-21 0.2697900941038682 1106 22-23 0.14285077246397293 1106 24-25 0.2749588286446638 1106 26-27 0.06044071135870155 1106 28-29 0.09711773355100206 1106 30-31 0.12125665018066911 1106 32-33 0.09245555155335694 1106 34-35 0.1675015660580783 1106 36-37 0.09954001128242851 1106 38-39 0.08483029522544427 1106 40-41 0.07921616509118934 1106 42-43 0.1428389723514627 1106 44-45 0.1127685847167541 1106 46-47 0.29716616626544123 1106 48-49 0.2987810233415189 1106 50-51 0.14581620924379735 1106 52-53 0.16838186225151475 1106 54-55 0.20357403164491927 1106 56-57 0.271649248327968 1106 58-59 0.27355892522729874 1106 60-61 0.21122661743110172 1106 62-63 0.2603570305771683 1106 64-65 0.0976042879722101 1106 66-67 0.18860900417898563 1106 68-69 0.13271052444552822 1106 70-71 0.19383120863621173 1106 72-73 0.27389616583991483 1106 74-75 0.16922666190203017 1106 76-77 0.23475029747223175 1106 78-79 0.2438786582856025 1106 80-81 0.2562624683158603 1106 82-83 0.19382414665049197 1106 84-85 0.17560359570413198 1106 86-87 0.11065909488200987 1106 88-89 0.035231337694256126 1106 90-91 0.04254068313247927 1106 92-93 -0.04267729572165635 1106 94-95 0.16225059320892754 1106 96-97 0.1843737773397045 1106 98-99 0.0714712542014162 1107 1 0.07348094015750206 1107 2 0.2136571088253163 1107 3 0.16982750309134786 1107 4 0.16234525201367944 1107 5 -0.006381832163448564 1107 6 0.058070703850148675 1107 7 0.07456575095813633 1107 8 0.01847340658334673 1107 9 0.27335315065217003 1107 10-11 0.16788198967298484 1107 12-13 0.09157364226886244 1107 14-15 0.22696648620174642 1107 16-17 0.21182878951147188 1107 18-19 0.07840348136709707 1107 20-21 0.17459185986216852 1107 22-23 0.06996528263413637 1107 24-25 0.1260519602325516 1107 26-27 -0.002265716438266452 1107 28-29 0.01525377939617556 1107 30-31 0.0012877698315492125 1107 32-33 -0.09812243548553568 1107 34-35 0.0031041623932424045 1107 36-37 0.02164258120564 1107 38-39 -0.023684163931555702 1107 40-41 -0.011716874011050038 1107 42-43 -0.0392409648258365 1107 44-45 -0.03300822596137465 1107 46-47 0.04080649627530164 1107 48-49 0.15865141164297825 1107 50-51 0.034561478698911685 1107 52-53 -0.020737320887853627 1107 54-55 0.015944651238449126 1107 56-57 0.03137570191093175 1107 58-59 0.04990015390952607 1107 60-61 0.09867262654716313 1107 62-63 0.12806101744085652 1107 64-65 -0.04493798631207113 1107 66-67 -0.06460732714566575 1107 68-69 -0.09757778509096227 1107 70-71 -0.059917911389653966 1107 72-73 0.03445560064663766 1107 74-75 -0.08576723816312892 1107 76-77 0.03353995042140667 1107 78-79 0.013363905868843062 1107 80-81 -0.04914018914157481 1107 82-83 -0.09266599964781363 1107 84-85 -0.07039362747193678 1107 86-87 -0.0158666458117267 1107 88-89 -0.0938526087054612 1107 90-91 -0.13593236440119938 1107 92-93 -0.1246830005862627 1107 94-95 0.05906385617670651 1107 96-97 -0.0022107115396003962 1107 98-99 -0.034306755957683066 1108 1 0.10725094354601339 1108 2 0.20030291343269369 1108 3 0.17190744880416986 1108 4 0.08745077883440189 1108 5 -0.03564350315987497 1108 6 0.044027041106907916 1108 7 0.029863323450548762 1108 8 -0.04430865833251829 1108 9 0.284676061302811 1108 10-11 0.18754863627144402 1108 12-13 0.16829723072775238 1108 14-15 0.17514978923740898 1108 16-17 0.1593444915543074 1108 18-19 -0.02396867880350584 1108 20-21 0.2001998067670101 1108 22-23 0.01214850148987523 1108 24-25 0.05910323308988552 1108 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2215 90-91 -0.31490055366462855 2215 92-93 -0.11142688195790029 2215 94-95 -0.12303366358679213 2215 96-97 -0.13580800019814987 2215 98-99 -0.03823169185487174 2216 1 -0.24982063270917365 2216 2 0.03754539640542731 2216 3 -0.12697937184818642 2216 4 -0.26686785176062955 2216 5 -0.2481136415424885 2216 6 -0.2233272229129497 2216 7 -0.2553435898375511 2216 8 -0.2508265580399751 2216 9 0.07708951738914038 2216 10-11 -0.2096877698040558 2216 12-13 -0.14489822625623816 2216 14-15 -0.44436448723833877 2216 16-17 -0.5305190828497501 2216 18-19 -0.4630540501631728 2216 20-21 -0.7952662983883698 2216 22-23 -0.6674476313393782 2216 24-25 -0.5087349667409313 2216 26-27 -0.5975937603153696 2216 28-29 -0.6598703272258106 2216 30-31 -0.3496490728612258 2216 32-33 -0.4037035445752011 2216 34-35 -0.3740248717299579 2216 36-37 -0.43527798286933717 2216 38-39 -0.45540007970866014 2216 40-41 -0.429161154912876 2216 42-43 -0.4920591213651946 2216 44-45 -0.38664218499562963 2216 46-47 -0.6398021646766239 2216 48-49 -0.7769481051821643 2216 50-51 -0.4270040912239992 2216 52-53 -0.4765251044548009 2216 54-55 -0.3278924796981997 2216 56-57 -0.3968696192021639 2216 58-59 -0.3614826337423622 2216 60-61 -0.4178120975640738 2216 62-63 -0.45038815820909406 2216 64-65 -0.39656481620273354 2216 66-67 -0.38010865239232317 2216 68-69 -0.35518984868742365 2216 70-71 -0.3666143591336919 2216 72-73 -0.2906573557220682 2216 74-75 -0.309915465761641 2216 76-77 -0.2927831363764426 2216 78-79 -0.28616519332604895 2216 80-81 -0.17394862118953824 2216 82-83 -0.14300899631117403 2216 84-85 -0.23192298935995126 2216 86-87 -0.3359319297300125 2216 88-89 -0.15293836062857835 2216 90-91 -0.21795703649853237 2216 92-93 -0.18827601993448084 2216 94-95 -0.26531123667244216 2216 96-97 -0.3203463415660899 2216 98-99 -0.1877929584696787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 32.0 10 95.0 11 189.0 12 284.0 13 502.0 14 1227.0 15 3165.0 16 6922.0 17 12637.0 18 20444.0 19 29772.0 20 38887.0 21 50184.0 22 60604.0 23 61392.0 24 55282.0 25 55222.0 26 65881.0 27 85676.0 28 112739.0 29 148220.0 30 196130.0 31 258529.0 32 340999.0 33 449902.0 34 596135.0 35 835138.0 36 1344912.0 37 1933182.0 38 655777.0 39 569.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.405351992387736 18.818214043829308 12.9585530980915 25.817880865691457 2 15.950960345744953 20.805923313579612 37.46365920596612 25.77945713470932 3 17.960158671035746 25.538282702452996 29.835340259244624 26.66621836726663 4 13.39745002988962 17.189510440884042 36.376625938800935 33.036413590425404 5 14.20911343890616 36.911907197253 33.53612372262134 15.342855641219492 6 31.668282880335326 36.96338157537979 17.143880928804485 14.224454615480397 7 27.21401507903096 31.806559437762477 22.36238241573903 18.61704306746753 8 27.15683669162589 32.5112833994283 20.03312115026259 20.29875875868322 9 26.964825617241317 13.882146512648047 19.738542096932544 39.41448577317809 10-11 25.56008676345262 24.816323021369058 27.37884596414647 22.244744251031854 12-13 26.673158473209035 22.75862203724856 27.17934823915363 23.38887125038878 14-15 24.107484396655753 23.47518400009595 25.305141760712583 27.11218984253571 16-17 23.487108559196507 26.045242578107576 25.93691153915247 24.53073732354345 18-19 23.784362957568597 24.939203324351176 28.35445641047479 22.92197730760544 20-21 24.856137667984253 24.313630260263405 27.629532023939728 23.200700047812614 22-23 24.343317742576243 24.003926348091998 27.608502829137553 24.044253080194206 24-25 23.94769127605774 24.521280925246838 27.643338118188254 23.88768968050717 26-27 24.09786708309099 24.510466488317174 27.498316855859795 23.89334957273204 28-29 23.71199045472652 24.44173248761966 27.932100429127637 23.914176628526178 30-31 24.16123092414181 24.29768149252975 27.7237624915169 23.817325091811536 32-33 23.92007908756069 24.468657403489406 27.288159667176775 24.32310384177313 34-35 24.256667487800236 24.430796767285177 27.63491565952029 23.677620085394295 36-37 24.35894982586398 24.330212396888893 27.22149422232811 24.08934355491901 38-39 24.265278609542364 24.055936714858404 27.434380287619554 24.24440438797968 40-41 24.21237883114062 24.22422417701124 27.510943805894804 24.052453185953336 42-43 24.584981664644378 24.253770162018455 27.074512211621755 24.086735961715412 44-45 24.257601497777774 24.56304202622068 26.66359503454811 24.51576144145344 46-47 24.239040966299925 24.538961350482627 26.627939977112476 24.594057706104973 48-49 24.8843764874062 24.74600559844197 25.959789699966418 24.409828214185413 50-51 24.297169407042738 25.117523619269296 25.72984984036409 24.85545713332388 52-53 24.315856144192708 24.987477469889665 25.65589151857155 25.040774867346077 54-55 24.47489676087063 24.93728300377488 25.69330310771206 24.89451712764243 56-57 24.54772366665975 24.767127179110403 25.472315214429504 25.212833939800344 58-59 24.1537989466132 24.7769665565054 26.038098351677675 25.031136145203725 60-61 24.277710158536276 24.93003968932043 25.849502657184644 24.942747494958653 62-63 24.049259389626442 24.916887177864538 25.677260008441326 25.356593424067697 64-65 23.93282732100052 24.683598548695826 25.837428220438248 25.54614590986541 66-67 24.429967997352247 24.723473837013433 25.71397519026671 25.13258297536761 68-69 24.270122987155503 24.445292392148215 25.829128670171986 25.455455950524296 70-71 23.892928835018548 24.519392999179583 25.895026774057573 25.6926513917443 72-73 24.352232072830414 24.519576219612443 25.89412147422404 25.2340702333331 74-75 24.419045752951636 24.57390444700427 25.789999671187214 25.217050128856876 76-77 24.151710176863546 24.712625710249096 25.73047647128898 25.405187641598374 78-79 24.1707023345749 24.786851847522065 25.75465748969272 25.28778832821032 80-81 23.903287554988154 24.733344469037473 25.914128897415033 25.449239078559337 82-83 24.077235428337946 24.63820486545897 25.76830341765854 25.516256288544543 84-85 24.076103449892976 24.754158538432556 25.45747426500909 25.712263746665382 86-87 23.82406305874591 24.645165185302176 25.671606854183388 25.859164901768526 88-89 23.90406294015769 24.3350367812139 25.76891657264957 25.99198370597884 90-91 23.993342596272907 24.468707797020205 25.623073734970998 25.914875871735894 92-93 23.885601363940097 24.546400504382383 25.55995601679954 26.008042114877977 94-95 23.355397607711406 24.51920621438206 26.001230629411964 26.124165548494577 96-97 23.80340668368816 24.47950935626188 25.65449901514274 26.06258494490722 98-99 23.54090673629592 24.502663922607173 25.730941391007455 26.225487950089455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 91.0 1 120.5 2 444.5 3 764.0 4 551.0 5 462.0 6 967.5 7 2613.0 8 4691.0 9 4667.5 10 2818.0 11 1659.5 12 1537.5 13 1717.5 14 2070.5 15 2549.0 16 3108.5 17 3735.5 18 4527.0 19 5452.0 20 6650.0 21 8149.0 22 10232.5 23 12950.5 24 15821.0 25 19037.0 26 23752.5 27 29430.5 28 36035.0 29 44106.0 30 51328.5 31 59840.0 32 69598.0 33 79756.0 34 89023.5 35 98382.0 36 107146.0 37 119361.5 38 136002.0 39 141973.0 40 146312.5 41 157701.0 42 170889.0 43 191708.0 44 217066.0 45 244513.5 46 276647.5 47 303518.0 48 320463.0 49 331542.0 50 344167.0 51 341181.5 52 333208.5 53 332558.0 54 318570.0 55 304800.0 56 283009.0 57 257815.5 58 242570.5 59 216244.5 60 181761.0 61 145265.5 62 118202.5 63 95462.0 64 72100.5 65 56039.5 66 41663.5 67 31988.0 68 25574.5 69 21518.0 70 18727.0 71 16318.5 72 15571.5 73 13045.5 74 9006.0 75 6834.5 76 5080.5 77 3389.5 78 2055.0 79 1307.5 80 815.5 81 545.0 82 324.0 83 192.0 84 126.0 85 71.5 86 40.5 87 24.5 88 10.5 89 3.0 90 3.0 91 2.5 92 2.0 93 2.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05636983137294431 2 0.008085560321244701 3 0.0 4 0.0 5 0.0022370050222110345 6 0.0 7 0.0 8 0.0 9 0.004325774771865915 10-11 0.0 12-13 0.0 14-15 0.002580641335863934 16-17 0.00437967850734088 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 5.727271894214997E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.4823527255615287E-4 54-55 0.0 56-57 8.355078998619526E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0014149730562178229 70-71 2.695186773748234E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.649197184715703E-4 80-81 8.152939990588408E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.4363631365289985E-4 92-93 0.0010174330070899585 94-95 5.794651563558703E-4 96-97 2.3582884270297047E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7420636.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.73779550303933 #Duplication Level Percentage of deduplicated Percentage of total 1 72.19924682589829 30.134373994928037 2 15.092666385258005 12.598692463669888 3 5.200006852458158 6.511104678669055 4 2.3506817480979145 3.9244909637935104 5 1.2970134920017404 2.7067241946925784 6 0.7806712704786617 1.955009870540177 7 0.5342161673530904 1.5607903603180506 8 0.386802810922491 1.2915437297826973 9 0.28902536541449037 1.0856953437175092 >10 1.5952229516993304 12.366178681728876 >50 0.13468322610359684 3.893957818270413 >100 0.1139803371728732 9.967913792496844 >500 0.0165816189075251 4.758950950098636 >1k 0.009038387311166548 6.409727671020114 >5k 3.2512184528145297E-5 0.12583339608944905 >10k+ 1.3004873811258119E-4 0.70901209018415 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19286 0.2598968605925422 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12342 0.1663199758080035 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 10511 0.14164554089433845 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10110 0.1362416914129732 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 9273 0.12496233476483687 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.347593386874117E-5 2 0.0 0.0 0.0 0.0 2.695186773748234E-5 3 0.0 0.0 0.0 1.347593386874117E-5 2.695186773748234E-5 4 0.0 0.0 0.0 2.695186773748234E-5 2.695186773748234E-5 5 0.0 0.0 0.0 4.042780160622351E-5 4.042780160622351E-5 6 0.0 0.0 0.0 4.042780160622351E-5 4.042780160622351E-5 7 0.0 1.347593386874117E-5 0.0 4.042780160622351E-5 4.042780160622351E-5 8 0.0 1.347593386874117E-5 0.0 4.042780160622351E-5 4.042780160622351E-5 9 0.0 1.347593386874117E-5 0.0 5.390373547496468E-5 4.042780160622351E-5 10-11 0.0 1.347593386874117E-5 0.0 9.433153708118819E-5 4.042780160622351E-5 12-13 0.0 1.347593386874117E-5 0.0 1.1454543788429994E-4 4.042780160622351E-5 14-15 0.0 1.347593386874117E-5 0.0 1.280213717530411E-4 6.064170240933526E-5 16-17 0.0 1.347593386874117E-5 0.0 1.4149730562178228E-4 8.085560321244702E-5 18-19 0.0 2.695186773748234E-5 0.0 1.751871402936352E-4 1.0106950401555877E-4 20-21 2.0213900803111754E-5 4.042780160622351E-5 0.0 2.897325781779351E-4 1.0780747094992936E-4 22-23 2.695186773748234E-5 6.737966934370585E-5 0.0 9.837431724181054E-4 1.2128340481867053E-4 24-25 3.3689834671852924E-5 7.411763627807643E-5 0.0 0.0026817108398794927 1.4823527255615287E-4 26-27 7.411763627807643E-5 8.085560321244702E-5 0.0 0.004426844275881475 1.4823527255615287E-4 28-29 8.085560321244702E-5 8.085560321244702E-5 0.0 0.011245666813464505 1.5497323949052345E-4 30-31 8.085560321244702E-5 8.085560321244702E-5 0.0 0.03310363154856268 1.6844917335926462E-4 32-33 1.0106950401555877E-4 8.085560321244702E-5 0.0 0.07459603193041675 1.8866307416237637E-4 34-35 1.1454543788429994E-4 9.433153708118819E-5 0.0 0.1298136709575837 1.8866307416237637E-4 36-37 1.2128340481867053E-4 1.1454543788429994E-4 0.0 0.2162415728247552 2.0887697496548813E-4 38-39 1.347593386874117E-4 1.2128340481867053E-4 0.0 0.348002516226372 2.156149418998587E-4 40-41 1.347593386874117E-4 1.2128340481867053E-4 0.0 0.5014866650243995 2.156149418998587E-4 42-43 1.347593386874117E-4 1.2128340481867053E-4 0.0 0.6472288898148353 2.223529088342293E-4 44-45 1.347593386874117E-4 1.2128340481867053E-4 0.0 0.8189594530711384 2.2909087576859988E-4 46-47 1.347593386874117E-4 1.2128340481867053E-4 0.0 1.0084849870011143 2.695186773748234E-4 48-49 1.347593386874117E-4 1.2128340481867053E-4 0.0 1.2083330862745458 2.8299461124356456E-4 50-51 1.347593386874117E-4 1.4823527255615287E-4 0.0 1.4304232143983344 2.8299461124356456E-4 52-53 1.347593386874117E-4 1.4823527255615287E-4 0.0 1.647614840560836 3.0320851204667634E-4 54-55 1.347593386874117E-4 1.4823527255615287E-4 0.0 1.8656082847885276 3.301603797841587E-4 56-57 1.347593386874117E-4 1.4823527255615287E-4 0.0 2.1133902808330713 3.503742805872704E-4 58-59 1.347593386874117E-4 1.4823527255615287E-4 0.0 2.3877198665990353 3.7058818139038214E-4 60-61 1.347593386874117E-4 1.4823527255615287E-4 0.0 2.663302714214792 3.7732614832475275E-4 62-63 1.347593386874117E-4 1.4823527255615287E-4 0.0 2.941897433050213 4.042780160622351E-4 64-65 1.347593386874117E-4 1.4823527255615287E-4 0.0 3.234143272894668 4.1775394993097626E-4 66-67 1.4823527255615287E-4 1.4823527255615287E-4 0.0 3.555874456043929 4.5818175153719976E-4 68-69 1.4823527255615287E-4 1.5497323949052345E-4 0.0 3.903001036568833 4.7165768540594093E-4 70-71 1.4823527255615287E-4 1.6171120642489403E-4 0.0 4.27968707803482 4.7165768540594093E-4 72-73 1.4823527255615287E-4 1.6171120642489403E-4 0.0 4.657996969531991 5.592512555527586E-4 74-75 1.4823527255615287E-4 1.6171120642489403E-4 0.0 5.035397235493022 5.659892224871291E-4 76-77 1.4823527255615287E-4 1.819251072280058E-4 0.0 5.442720812609593 5.659892224871291E-4 78-79 1.4823527255615287E-4 1.8866307416237637E-4 0.0 5.867448558317642 5.727271894214997E-4 80-81 1.4823527255615287E-4 1.8866307416237637E-4 0.0 6.331162180707961 5.996790571589821E-4 82-83 1.4823527255615287E-4 1.8866307416237637E-4 0.0 6.822339756322774 6.333688918308349E-4 84-85 1.4823527255615287E-4 1.8866307416237637E-4 0.0 7.308025888886074 6.468448256995761E-4 86-87 1.4823527255615287E-4 1.8866307416237637E-4 0.0 7.811136403941656 6.535827926339468E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 34430 0.0 34.785683 1 CAACGCA 43630 0.0 27.31656 5 ATCAACG 43830 0.0 27.149475 3 AACGCAG 46200 0.0 26.22892 6 TCAACGC 45965 0.0 25.949123 4 TATCAAC 46205 0.0 25.94587 2 GGTATCA 21695 0.0 25.457754 1 ACGCAGA 49140 0.0 23.959433 7 CGCAGAG 49895 0.0 23.560558 8 AGTACTT 28475 0.0 22.382881 12-13 GCAGAGT 53400 0.0 21.892797 9 AGAGTAC 50495 0.0 20.215607 10-11 TAGTACT 3235 0.0 19.980352 4 GAGTACT 27390 0.0 19.551163 12-13 TACTTTT 33195 0.0 19.50107 14-15 GTACTAG 2485 0.0 19.285894 1 CGTTAAC 1115 0.0 18.361443 1 GTATAAG 2650 0.0 18.26066 1 GTACTTT 30670 0.0 17.71768 14-15 CTAGTAC 3615 0.0 17.108265 3 >>END_MODULE