FastQCFastQC Report
Fri 27 May 2016
ERR765434_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765434_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6002758
Sequences flagged as poor quality0
Sequence length99
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA97230.16197554524103752No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT91030.15164695961423066No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG60760.1012201391427074No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13800.021.2283381
GTATTAC10450.020.0239981
GTATAAG21600.019.590351
CGTTAAC4850.019.175331
TAGTACT20250.018.3701324
ATGGGTA34950.018.227235
GTACATG343100.017.3748821
TACATGG334850.017.3166332
GTATCAA232550.016.8564381
GTCCTAC18100.016.6989561
GTATATA15900.016.6698721
ATAGGAC47600.016.6069283
CTAGTAC20500.016.5583213
CATGGGT48900.016.5457974
ACATGGG321000.016.3979173
CATGGGG121950.016.3958514
GTACATA16750.015.5463261
GGGTACC40150.015.51917
GTATTAG32250.015.2837761
GTATATG19050.015.1339151
Produced by FastQC (version 0.11.5)