##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765434_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6002758 Sequences flagged as poor quality 0 Sequence length 99 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.859351651357592 33.0 33.0 33.0 27.0 33.0 2 30.794438822954383 33.0 33.0 33.0 27.0 33.0 3 30.855064788552195 33.0 33.0 33.0 27.0 33.0 4 34.43841530842989 37.0 37.0 37.0 27.0 37.0 5 34.508808284458574 37.0 37.0 37.0 27.0 37.0 6 34.452891487546225 37.0 37.0 37.0 27.0 37.0 7 34.306831792985825 37.0 33.0 37.0 27.0 37.0 8 34.237646261934934 37.0 33.0 37.0 27.0 37.0 9 34.283990125872144 37.0 37.0 37.0 27.0 37.0 10-11 34.20053515400754 37.0 33.0 37.0 27.0 37.0 12-13 34.12577335284881 37.0 33.0 37.0 27.0 37.0 14-15 35.31571945762265 37.0 33.0 40.0 27.0 40.0 16-17 35.324744309199204 37.0 33.0 40.0 27.0 40.0 18-19 35.302239987019306 37.0 33.0 40.0 27.0 40.0 20-21 35.27586211204916 37.0 33.0 40.0 27.0 40.0 22-23 35.27939290572767 37.0 33.0 40.0 27.0 40.0 24-25 35.18522285922571 37.0 33.0 40.0 27.0 40.0 26-27 35.17127627000788 37.0 33.0 40.0 27.0 40.0 28-29 35.055642922803145 37.0 33.0 40.0 27.0 40.0 30-31 34.999573196187484 37.0 33.0 40.0 27.0 40.0 32-33 34.85626173835427 37.0 33.0 40.0 27.0 40.0 34-35 34.792008939890636 37.0 33.0 40.0 27.0 40.0 36-37 34.66228198771299 37.0 33.0 40.0 27.0 40.0 38-39 34.42730941343962 37.0 33.0 40.0 22.0 40.0 40-41 34.352693128725164 37.0 33.0 40.0 22.0 40.0 42-43 34.203232247576864 37.0 33.0 40.0 22.0 40.0 44-45 34.06022756539578 37.0 33.0 40.0 22.0 40.0 46-47 33.90831681037283 37.0 33.0 40.0 22.0 40.0 48-49 33.72217687269752 37.0 33.0 40.0 22.0 40.0 50-51 33.53942421133752 37.0 33.0 37.0 22.0 40.0 52-53 33.32765039003738 37.0 33.0 37.0 22.0 40.0 54-55 33.18296106556353 37.0 33.0 37.0 22.0 40.0 56-57 32.98030330391464 37.0 33.0 37.0 22.0 40.0 58-59 32.738355102771095 37.0 33.0 37.0 22.0 40.0 60-61 32.49576136502588 37.0 33.0 37.0 22.0 40.0 62-63 32.32329514199972 37.0 33.0 37.0 22.0 40.0 64-65 32.0013297221044 33.0 27.0 37.0 22.0 40.0 66-67 31.771603319674057 33.0 27.0 37.0 22.0 37.0 68-69 31.467200160326303 33.0 27.0 37.0 22.0 37.0 70-71 31.330854167367733 33.0 27.0 37.0 22.0 37.0 72-73 31.047813938193077 33.0 27.0 37.0 18.5 37.0 74-75 30.76769719852108 33.0 27.0 37.0 15.0 37.0 76-77 30.59201437072759 33.0 27.0 37.0 15.0 37.0 78-79 30.356607579382676 33.0 27.0 37.0 15.0 37.0 80-81 30.082599031978297 33.0 27.0 37.0 15.0 37.0 82-83 29.924742010255954 33.0 27.0 37.0 15.0 37.0 84-85 29.743722468905126 33.0 27.0 37.0 15.0 37.0 86-87 29.538514379556865 33.0 27.0 37.0 15.0 37.0 88-89 29.353478601003072 33.0 27.0 37.0 15.0 37.0 90-91 29.156557702309506 33.0 27.0 33.0 15.0 37.0 92-93 29.012941634495345 33.0 27.0 33.0 15.0 37.0 94-95 28.75266469179667 33.0 27.0 33.0 15.0 37.0 96-97 28.686828621110493 33.0 27.0 33.0 15.0 37.0 98-99 27.54533299526651 33.0 24.5 33.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3680751031947196 1101 2 0.25925044851488366 1101 3 0.41275759839803783 1101 4 0.45051121743185973 1101 5 0.4490907773598636 1101 6 0.37771681974633964 1101 7 0.2565071329034794 1101 8 0.3621295805143703 1101 9 0.30838326198980326 1101 10-11 0.2783384434551621 1101 12-13 0.30887113852915604 1101 14-15 0.44928739590373823 1101 16-17 0.4309922935690622 1101 18-19 0.4502367313294897 1101 20-21 0.471450612142128 1101 22-23 0.3580574649849808 1101 24-25 0.46572802924901424 1101 26-27 0.5175402502214865 1101 28-29 0.3963794465566153 1101 30-31 0.4384242319746434 1101 32-33 0.5146457671240086 1101 34-35 0.5429268857235812 1101 36-37 0.4843588582688554 1101 38-39 0.44861855031562214 1101 40-41 0.4198201366198262 1101 42-43 0.436605732684086 1101 44-45 0.3841744599294401 1101 46-47 0.4127173247669518 1101 48-49 0.5204407286610788 1101 50-51 0.4933442333490916 1101 52-53 0.398654068452565 1101 54-55 0.5125312749422264 1101 56-57 0.5968764066165804 1101 58-59 0.48038993439988786 1101 60-61 0.6326751406430873 1101 62-63 0.4881171435928593 1101 64-65 0.42979824116318355 1101 66-67 0.4898876638619001 1101 68-69 0.5168070657987904 1101 70-71 0.5415336089180407 1101 72-73 0.6275756313105347 1101 74-75 0.49032201079379334 1101 76-77 0.3709812149183094 1101 78-79 0.4080023465471534 1101 80-81 0.3765474482075213 1101 82-83 0.5133904601659012 1101 84-85 0.39894821096749666 1101 86-87 0.4530531993571998 1101 88-89 0.4429554677929808 1101 90-91 0.4896870462371581 1101 92-93 0.5338317569911126 1101 94-95 0.566491896513547 1101 96-97 0.3635963037989498 1101 98-99 0.4380202497971446 1102 1 0.2877103270826282 1102 2 0.32849169544443413 1102 3 0.36141537907530363 1102 4 0.38175972314795814 1102 5 0.39291226905863397 1102 6 0.3047356260260372 1102 7 0.3207974955962314 1102 8 0.345049679179958 1102 9 0.31858929321455776 1102 10-11 0.3478368334495201 1102 12-13 0.3856539806958068 1102 14-15 0.553523710436906 1102 16-17 0.5554666435794786 1102 18-19 0.5399459514656257 1102 20-21 0.5748526183991913 1102 22-23 0.4633027148009532 1102 24-25 0.6068084854681146 1102 26-27 0.6161926611565036 1102 28-29 0.42486188743153974 1102 30-31 0.4897552883923453 1102 32-33 0.5933801364247415 1102 34-35 0.4587490548499318 1102 36-37 0.5574084389342175 1102 38-39 0.5632137237154993 1102 40-41 0.5165128520272688 1102 42-43 0.4644056909805627 1102 44-45 0.434243457098205 1102 46-47 0.5878633216393965 1102 48-49 0.535299772304441 1102 50-51 0.5332816431896674 1102 52-53 0.4539999044206766 1102 54-55 0.5300074820479495 1102 56-57 0.46135711495431053 1102 58-59 0.45836759459658083 1102 60-61 0.5942345563598863 1102 62-63 0.5878870620816912 1102 64-65 0.5156358692147762 1102 66-67 0.4651693749409631 1102 68-69 0.5085658473765378 1102 70-71 0.5078339421001914 1102 72-73 0.4836389658296767 1102 74-75 0.45152269082075946 1102 76-77 0.382857804316167 1102 78-79 0.4636449957821185 1102 80-81 0.5091337035557792 1102 82-83 0.458726212385276 1102 84-85 0.3949508210947066 1102 86-87 0.523268421071851 1102 88-89 0.5521710832887301 1102 90-91 0.5716600278859971 1102 92-93 0.6441136393630273 1102 94-95 0.6024462833438875 1102 96-97 0.5531862393509499 1102 98-99 0.5727231096813341 1103 1 0.12358973414122332 1103 2 0.23559850251763237 1103 3 0.24051507659151383 1103 4 0.2935978933249288 1103 5 0.2706597502619772 1103 6 0.22409839309488433 1103 7 0.09814880784138325 1103 8 0.16956389225889978 1103 9 0.30176858846049015 1103 10-11 0.1654597919325198 1103 12-13 0.1970214117795024 1103 14-15 0.24797218445537794 1103 16-17 0.18309976711395848 1103 18-19 0.2756303665783548 1103 20-21 0.326231684439378 1103 22-23 0.25078231970105236 1103 24-25 0.35778470482809865 1103 26-27 0.373452965142441 1103 28-29 0.313899818930345 1103 30-31 0.4314993297850904 1103 32-33 0.4707780897141447 1103 34-35 0.4235098010502014 1103 36-37 0.43322267818884086 1103 38-39 0.4373572448460763 1103 40-41 0.36075415207180583 1103 42-43 0.3277904603148656 1103 44-45 0.28153648953574617 1103 46-47 0.32794616547652566 1103 48-49 0.3958709578175359 1103 50-51 0.4170792256258906 1103 52-53 0.4164841229280114 1103 54-55 0.4060731155905515 1103 56-57 0.3456306642309528 1103 58-59 0.44184564064495646 1103 60-61 0.4077881016469149 1103 62-63 0.3896073626275509 1103 64-65 0.3370761748027462 1103 66-67 0.38226519310826035 1103 68-69 0.3312048846810214 1103 70-71 0.19538100050206353 1103 72-73 0.3092871208223329 1103 74-75 0.3054829875310716 1103 76-77 0.2549134624348923 1103 78-79 0.34811216988549276 1103 80-81 0.34193160055256655 1103 82-83 0.27837977386948154 1103 84-85 0.24703010294265226 1103 86-87 0.49828072458430483 1103 88-89 0.4415992315709758 1103 90-91 0.38424212361448085 1103 92-93 0.4547636184128514 1103 94-95 0.4636540693975597 1103 96-97 0.32149560361099105 1103 98-99 0.4181393932056743 1104 1 0.13142631756442924 1104 2 0.11612569300279674 1104 3 0.054626151323798666 1104 4 0.16593223324674966 1104 5 0.14837556803276897 1104 6 0.08377097956459068 1104 7 -0.07686228699483166 1104 8 -0.012895370275948892 1104 9 -0.007604286504459878 1104 10-11 0.07322626919980735 1104 12-13 0.020248737926472415 1104 14-15 0.060401796304290656 1104 16-17 0.0793321084854881 1104 18-19 0.11801824336872357 1104 20-21 0.12964142821345348 1104 22-23 0.09551737791733927 1104 24-25 0.09475693072073454 1104 26-27 0.12617937617252295 1104 28-29 5.53654684274818E-4 1104 30-31 0.04323444142726629 1104 32-33 0.15128396544952238 1104 34-35 0.10511426075449037 1104 36-37 0.15929832692866341 1104 38-39 0.1360076658824525 1104 40-41 0.11618419890819354 1104 42-43 0.031756308781716314 1104 44-45 0.029228464018963507 1104 46-47 -0.07449637911355467 1104 48-49 0.03471459445633229 1104 50-51 0.0923348789311973 1104 52-53 -0.06645624720125909 1104 54-55 0.13821336839168197 1104 56-57 0.0677907901453807 1104 58-59 0.16342104361208243 1104 60-61 0.10930565327609543 1104 62-63 0.018675350086219566 1104 64-65 0.05035841182130696 1104 66-67 0.03161181086156262 1104 68-69 -0.07476938560285262 1104 70-71 -0.02837933169891471 1104 72-73 0.03400356417984085 1104 74-75 -0.06873497233632975 1104 76-77 -0.13675200400282606 1104 78-79 -0.09296691908195598 1104 80-81 -0.0910025429871304 1104 82-83 0.021232007627570937 1104 84-85 0.04859967785825248 1104 86-87 0.03433206545376066 1104 88-89 0.08667687363650955 1104 90-91 0.061071400764056705 1104 92-93 0.1216885555875038 1104 94-95 0.13247575603145023 1104 96-97 -0.0780695745856761 1104 98-99 -0.10314121643407148 1105 1 -0.015535699289880966 1105 2 0.07319105584511121 1105 3 0.08349024222592405 1105 4 0.008163491122019195 1105 5 0.05877583807632192 1105 6 0.024103159255467688 1105 7 -0.08212148952292608 1105 8 -0.10710816715989324 1105 9 -0.11969712898084595 1105 10-11 -0.057294031775086296 1105 12-13 -0.09435281246448568 1105 14-15 0.08653419583947652 1105 16-17 0.05667238270281416 1105 18-19 0.05323852204639934 1105 20-21 0.08663290582274641 1105 22-23 0.0035730390528172506 1105 24-25 -0.02352446813594611 1105 26-27 0.024852183364799885 1105 28-29 0.006167875841832426 1105 30-31 0.07049819976504068 1105 32-33 0.10679556879287588 1105 34-35 -0.044069273802961106 1105 36-37 0.007790902064471084 1105 38-39 -0.05178808127516987 1105 40-41 -0.10939150248793084 1105 42-43 -0.04904686918391832 1105 44-45 -0.14199614803864335 1105 46-47 -0.15949199741469755 1105 48-49 -0.12171342759717163 1105 50-51 0.059433604428157594 1105 52-53 -0.05918834039719911 1105 54-55 0.06619719389320977 1105 56-57 0.04280195909304041 1105 58-59 -0.04864199956048765 1105 60-61 0.08740019871190441 1105 62-63 0.08733435406830381 1105 64-65 -0.1841006427306624 1105 66-67 -0.10775688098255998 1105 68-69 -0.15688468326886706 1105 70-71 -0.20368612406890207 1105 72-73 -0.11305960487504052 1105 74-75 -0.029680002039356168 1105 76-77 -0.15080270740922685 1105 78-79 -0.022052836313587676 1105 80-81 -0.13382572231870782 1105 82-83 -0.0447516921272495 1105 84-85 -0.13627780882025675 1105 86-87 -0.05621436954787029 1105 88-89 -0.05745842991546013 1105 90-91 -0.014830310959663251 1105 92-93 0.09359029018248322 1105 94-95 -0.01212454932242224 1105 96-97 -0.11691557911499473 1105 98-99 0.03988083661016262 1106 1 0.07970354536938018 1106 2 0.16049463177743206 1106 3 0.16537320184660587 1106 4 0.12890442950330794 1106 5 0.1202534852226762 1106 6 0.0849020835088723 1106 7 -0.01028544432773515 1106 8 0.002798508053906801 1106 9 -0.0032245827436696572 1106 10-11 -0.00576099343617642 1106 12-13 0.04870262536127257 1106 14-15 -0.02942223334254379 1106 16-17 0.06358744787621617 1106 18-19 0.11678599213062313 1106 20-21 0.06775242260063408 1106 22-23 0.057991720630219845 1106 24-25 0.1125854353430853 1106 26-27 0.10970561826219694 1106 28-29 0.048248906064117136 1106 30-31 0.05866979589005439 1106 32-33 0.1954791302637915 1106 34-35 -0.0630840466742768 1106 36-37 -7.078165655656221E-4 1106 38-39 0.05278335900926123 1106 40-41 0.1047959767160691 1106 42-43 0.09178263211401116 1106 44-45 0.13225807413645896 1106 46-47 -0.03135369198282234 1106 48-49 -0.006729575981587743 1106 50-51 0.046118764345990826 1106 52-53 -0.017788441303380864 1106 54-55 0.02170914336918628 1106 56-57 0.057164414918844386 1106 58-59 0.06673944397050491 1106 60-61 0.1076984612332339 1106 62-63 0.021477386122938924 1106 64-65 -0.06730018248911307 1106 66-67 0.013247143256673866 1106 68-69 -0.03490527370473728 1106 70-71 -0.059382099361336316 1106 72-73 0.03481273125302309 1106 74-75 0.01433984088908602 1106 76-77 0.02214672943321716 1106 78-79 -2.8214308379403974E-4 1106 80-81 -0.04866720726291973 1106 82-83 0.0837455236306539 1106 84-85 -0.057838942278390704 1106 86-87 -0.11654994706816169 1106 88-89 0.15649803069569046 1106 90-91 0.07814385502121013 1106 92-93 0.02327184398402693 1106 94-95 -0.09080738565920754 1106 96-97 -0.19294015070821047 1106 98-99 0.2582966557832336 1107 1 0.04295581073361987 1107 2 0.09251668480289155 1107 3 0.034172151776253656 1107 4 0.025521988979868127 1107 5 0.11678105990442589 1107 6 -0.034976433279950925 1107 7 -0.10350423399376751 1107 8 -0.1486346738490738 1107 9 -0.16852226613106325 1107 10-11 -0.058053611270253214 1107 12-13 -0.13250775698680428 1107 14-15 0.02227164762983591 1107 16-17 -0.06677749036055758 1107 18-19 -0.15583510604714235 1107 20-21 -0.10233826717255567 1107 22-23 -0.09508542863247982 1107 24-25 0.04214486871462242 1107 26-27 0.10263762729753267 1107 28-29 0.0437913251365174 1107 30-31 0.030691741607512313 1107 32-33 -0.11398963312497301 1107 34-35 -0.09378871368392083 1107 36-37 -0.08986332723158341 1107 38-39 -0.1534372637764747 1107 40-41 -0.25736894195897975 1107 42-43 -0.24464801397778047 1107 44-45 -0.09008892906334154 1107 46-47 -0.32106662373151096 1107 48-49 -0.1591152043442534 1107 50-51 -0.17875600194027186 1107 52-53 -0.19233444710170744 1107 54-55 -0.18595857617660982 1107 56-57 -0.05649526242007141 1107 58-59 0.012100123311071798 1107 60-61 0.03671945252519038 1107 62-63 -0.24289025354875804 1107 64-65 -0.31598156946788336 1107 66-67 -0.14772575285343947 1107 68-69 -0.12140398781185979 1107 70-71 -0.07261447316471248 1107 72-73 -0.15118406067755785 1107 74-75 -0.174699396720996 1107 76-77 -0.2785655142249013 1107 78-79 -0.10851116703045349 1107 80-81 -0.33063229249405524 1107 82-83 -0.20184767147648586 1107 84-85 -0.30810009872615396 1107 86-87 -0.18091179034869498 1107 88-89 -0.1636053513270319 1107 90-91 0.014961663100500289 1107 92-93 0.013132139522575415 1107 94-95 -0.04051516046686032 1107 96-97 -0.20049162138634102 1107 98-99 0.13624992367686772 1108 1 -0.025401542266230592 1108 2 -0.0983655103349399 1108 3 -0.09010481706292595 1108 4 -0.1398333606895008 1108 5 -0.029201980734065103 1108 6 -0.282820245599666 1108 7 -0.1363540503551377 1108 8 -0.22694533146925977 1108 9 -0.1721368094564113 1108 10-11 -0.1926885581875979 1108 12-13 -0.2246392162751647 1108 14-15 -0.22412238374727167 1108 16-17 -0.1975660269983308 1108 18-19 -0.1966284873918056 1108 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0.19189142726328612 2215 50-51 0.09335115241637482 2215 52-53 0.09101935595161592 2215 54-55 0.10113167060853101 2215 56-57 0.15032440019886906 2215 58-59 -0.03765361423444347 2215 60-61 0.030301018600681573 2215 62-63 0.05141462199654967 2215 64-65 0.012147439656928327 2215 66-67 -0.1154378068272166 2215 68-69 0.09272650052714226 2215 70-71 -0.0863456609352724 2215 72-73 -0.08216496204979151 2215 74-75 0.16460903010184325 2215 76-77 0.10868415662746855 2215 78-79 0.13153809427473107 2215 80-81 0.25828189316292693 2215 82-83 0.17786967054256309 2215 84-85 0.20933645138533308 2215 86-87 0.1511775234438275 2215 88-89 -0.03221955231206408 2215 90-91 0.1730162430365496 2215 92-93 0.1772344573900284 2215 94-95 0.23790122977631967 2215 96-97 0.19969198468610472 2215 98-99 0.07616905520837847 2216 1 -0.03359538504333415 2216 2 -0.029618181737930627 2216 3 0.01609384867957786 2216 4 0.007972623253202471 2216 5 -0.1228681564401839 2216 6 -0.007185462488706662 2216 7 -0.03236297428513524 2216 8 0.02460437106640967 2216 9 0.0921514421464309 2216 10-11 -0.03180526239830783 2216 12-13 0.03051168189384157 2216 14-15 -0.02924931148943699 2216 16-17 -0.050333311697698946 2216 18-19 -0.12751387698344274 2216 20-21 -0.06256829632847172 2216 22-23 -0.17693260262175414 2216 24-25 -0.1662024181517623 2216 26-27 -0.20791645525167013 2216 28-29 -0.03242645084804252 2216 30-31 -0.10859069615396777 2216 32-33 -0.11736081631732986 2216 34-35 -0.22277063413197595 2216 36-37 -0.1885731177604768 2216 38-39 -0.10151232465806714 2216 40-41 -0.06756138543637036 2216 42-43 -0.11479874095445552 2216 44-45 -0.10800954042502298 2216 46-47 0.026963238270205636 2216 48-49 -0.008809283339019203 2216 50-51 -0.007158718676343767 2216 52-53 0.20810154676929926 2216 54-55 0.06484530103229957 2216 56-57 0.20087746262568373 2216 58-59 -0.14493694841632987 2216 60-61 0.022170840913737777 2216 62-63 0.02921086993880806 2216 64-65 0.10284652588686072 2216 66-67 0.030425656189219552 2216 68-69 -0.04857771290084045 2216 70-71 -0.07498054566544354 2216 72-73 -0.059108850894233456 2216 74-75 0.01706206270808508 2216 76-77 0.011763990892994514 2216 78-79 0.03690424477889209 2216 80-81 0.13590221187932983 2216 82-83 0.04356793246412494 2216 84-85 0.12580117462379903 2216 86-87 0.003453984348574579 2216 88-89 -0.08531195728022212 2216 90-91 0.17420216821359347 2216 92-93 0.2577002764737415 2216 94-95 0.16297826624226985 2216 96-97 0.24154376919249998 2216 98-99 0.15780818027777244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 6.0 7 172.0 8 1155.0 9 2303.0 10 4763.0 11 9800.0 12 17669.0 13 26733.0 14 34444.0 15 38352.0 16 41701.0 17 44969.0 18 47611.0 19 52205.0 20 56810.0 21 62928.0 22 69725.0 23 78436.0 24 87838.0 25 98485.0 26 111145.0 27 127001.0 28 149764.0 29 179100.0 30 217050.0 31 269084.0 32 340554.0 33 435117.0 34 568219.0 35 767582.0 36 1004488.0 37 884047.0 38 173412.0 39 90.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.030963197675675 18.20881249989588 12.313693543308661 25.446530759119785 2 16.580611778785684 20.48986482546856 36.638958292171694 26.29056510357406 3 18.341791670359413 25.204222860299502 28.499425930355986 27.95455953898509 4 13.403338931870984 16.8797242867362 35.706736803316076 34.01019997807674 5 15.36155547166819 36.46010717073718 32.668100229927646 15.510237127666981 6 32.931295914311384 35.72452862500871 17.169790952758714 14.174384507921193 7 28.92058950235875 30.951755842897548 21.555758203145952 18.57189645159775 8 27.385844973260625 32.39027793557561 19.943715871937535 20.28016121922623 9 27.22455244739168 15.140457103218221 18.903610640309005 38.73137980908109 10-11 26.098753273078806 24.750481362067237 26.480269569421257 22.6704957954327 12-13 26.62007197358281 22.756564565821243 25.97997453837053 24.64338892222542 14-15 24.671109513327043 24.0707188262462 23.02138450358985 28.236787156836908 16-17 25.024290919631518 26.205306477897867 23.921439438458076 24.848963164012538 18-19 25.41806616225408 25.53627016114926 24.932306116621728 24.113357559974933 20-21 26.477852320594618 25.05620142270042 23.876623331730453 24.589322924974503 22-23 25.56847202569219 24.738478546028343 24.04170716194123 25.65134226633824 24-25 25.23951710738903 25.326822484120765 23.963197630851443 25.470462777638765 26-27 25.447862919666502 25.309201646076822 23.88046660222406 25.362468832032615 28-29 24.973026691746234 25.141772307958878 24.50486735763208 25.380333642662812 30-31 25.431135478564777 25.177608934076517 24.193680719479964 25.197574867878743 32-33 24.978795154991737 25.21243785458195 23.817844988022586 25.990922002403728 34-35 25.552448739548854 25.11191742653348 24.359987249180648 24.975646584737017 36-37 25.450026471165422 25.274057358300965 23.88194726490723 25.39396890562638 38-39 25.453102178457147 24.86155737592265 24.038327385372472 25.647013060247726 40-41 25.28912543200975 25.300445228676548 24.37642830179061 25.034001037523083 42-43 25.840388701326955 25.089950319503135 24.15969459371842 24.909966385451487 44-45 25.232768004307353 25.329323620908923 23.89984737015885 25.538061004624872 46-47 24.88060488195593 25.252917075784165 24.51135794579758 25.355120096462326 48-49 25.559175473818684 25.405604053279298 24.056612771041646 24.978607701860376 50-51 24.847911576645267 25.658505640240705 24.096232098645324 25.397350684468705 52-53 25.03627499226189 25.11215677860077 24.179102339291372 25.67246588984597 54-55 25.316621126488858 24.989896310995714 24.34160264331829 25.35187991919714 56-57 25.208320908489064 24.72583435814004 24.003516383635656 26.062328349735235 58-59 24.988263728106315 24.796710112251734 24.527184004419304 25.68784215522265 60-61 25.005547450022142 24.92078641184602 24.38115113086351 25.692515007268323 62-63 24.58128413639197 25.13386346742614 24.209854870044737 26.07499752613715 64-65 24.77750227478769 24.650135820900992 24.37698637859464 26.19537552571668 66-67 25.128640910941574 24.721361321366388 24.29795580563328 25.85204196205876 68-69 25.244271050073984 24.38493272592365 24.317072252454622 26.053723971547743 70-71 24.52836679406366 24.400958692654275 24.64928621143814 26.42138830184392 72-73 25.033009826483095 24.569439580939296 24.54508410967092 25.85246648290669 74-75 25.24392954038793 24.213778066682014 24.629670228251747 25.9126221646783 76-77 24.858656637498964 24.513398674409327 24.76377525130948 25.864169436782227 78-79 24.98783059387035 24.399742584991767 24.843205406581443 25.76922141455644 80-81 24.602865882649276 24.185407774226448 25.17790988742175 26.033816455702528 82-83 24.806513939092664 24.530349216143644 24.64001547288763 26.023121371876062 84-85 24.696881000366833 24.485161654026367 24.566607549396462 26.251349796210345 86-87 24.648869736211253 24.130532998331766 24.67548250320936 26.545114762247618 88-89 24.671934134276277 23.874617300914014 24.957527856362024 26.495920708447684 90-91 25.056432393243238 24.040832563964763 24.681779608639896 26.2209554341521 92-93 24.70985836843664 24.228238086559546 24.64789518418034 26.414008360823477 94-95 24.165841768067278 24.270168812402567 25.127258170327703 26.43673124920245 96-97 24.8717506186323 24.117339063143977 24.703028174715687 26.30788214350803 98-99 24.46312178501949 24.104220093497023 24.7724962425605 26.660161878922988 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 601.0 1 575.0 2 563.0 3 564.5 4 529.0 5 455.5 6 398.0 7 376.5 8 350.0 9 328.0 10 324.0 11 331.5 12 351.5 13 389.0 14 422.5 15 480.0 16 537.5 17 637.0 18 813.0 19 1049.5 20 1437.5 21 2064.0 22 2921.0 23 4096.5 24 5588.0 25 7629.0 26 10450.0 27 13499.5 28 17448.0 29 22269.5 30 27168.5 31 32942.0 32 39006.5 33 45781.5 34 52625.0 35 59357.5 36 66245.0 37 74748.0 38 86103.5 39 94102.5 40 101195.5 41 110786.0 42 125828.0 43 147729.5 44 167781.5 45 185914.5 46 216825.0 47 245170.0 48 263534.0 49 278675.5 50 290865.5 51 288270.5 52 280432.5 53 282948.0 54 276906.0 55 268692.5 56 252110.0 57 238731.0 58 228049.0 59 203448.5 60 173929.0 61 138357.0 62 113995.0 63 93512.0 64 71603.0 65 56815.5 66 41624.0 67 31306.5 68 25254.0 69 21845.0 70 19391.0 71 16965.5 72 16229.0 73 14596.5 74 11048.5 75 8322.5 76 6069.5 77 4159.5 78 2754.5 79 1810.5 80 1213.5 81 810.0 82 552.5 83 394.0 84 283.0 85 209.0 86 157.0 87 110.0 88 76.5 89 59.5 90 45.5 91 30.0 92 18.0 93 11.0 94 7.5 95 7.0 96 7.0 97 7.0 98 5.0 99 8.5 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0024655333431732544 2 0.0 3 3.331801815098993E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 8.329504537747482E-6 18-19 0.0 20-21 3.081916678966568E-4 22-23 0.0 24-25 0.0043979783959306705 26-27 8.329504537747482E-6 28-29 0.008795956791861341 30-31 5.580768040290813E-4 32-33 4.164752268873741E-5 34-35 1.0828355899071726E-4 36-37 0.0 38-39 8.329504537747482E-6 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 2.2489662251918202E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.331801815098993E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6002758.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.76263051216307 #Duplication Level Percentage of deduplicated Percentage of total 1 78.2311991224381 37.36527858208467 2 12.198587690334636 11.652732732473478 3 3.999903669578473 5.7313776316296545 4 1.7353348965369233 3.3153663791262318 5 0.9733849846232687 2.3245713683324363 6 0.5987771114078139 1.7159501958787033 7 0.39852439574573617 1.3324201424860656 8 0.294313401828914 1.124574581306576 9 0.21624863825464694 0.9295743426943602 >10 1.1557346037674545 10.087936191321667 >50 0.09496417339181269 3.1752275524633964 >100 0.08072913102475626 8.005596046467863 >500 0.013097998071102276 4.362490513177423 >1k 0.008954376580565828 8.111063219164 >5k 2.458064159336249E-4 0.7658405213935129 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 9723 0.16197554524103752 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9103 0.15164695961423066 No Hit GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG 6076 0.1012201391427074 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.663603630197986E-5 0.0 5 0.0 0.0 0.0 8.329504537747482E-5 0.0 6 4.9977027226484894E-5 0.0 0.0 8.329504537747482E-5 1.6659009075494965E-5 7 4.9977027226484894E-5 0.0 0.0 8.329504537747482E-5 1.6659009075494965E-5 8 4.9977027226484894E-5 0.0 0.0 8.329504537747482E-5 1.6659009075494965E-5 9 4.9977027226484894E-5 0.0 0.0 8.329504537747482E-5 3.331801815098993E-5 10-11 4.9977027226484894E-5 0.0 0.0 1.1661306352846475E-4 3.331801815098993E-5 12-13 4.9977027226484894E-5 0.0 0.0 1.2494256806621224E-4 3.331801815098993E-5 14-15 4.9977027226484894E-5 0.0 0.0 1.3327207260395972E-4 4.1647522688737415E-5 16-17 4.9977027226484894E-5 0.0 0.0 1.7491959529269713E-4 4.9977027226484894E-5 18-19 6.663603630197986E-5 8.329504537747482E-6 0.0 2.0823761344368707E-4 4.9977027226484894E-5 20-21 6.663603630197986E-5 1.6659009075494965E-5 0.0 3.9981621781187915E-4 4.9977027226484894E-5 22-23 6.663603630197986E-5 1.6659009075494965E-5 0.0 0.0012327666715866274 4.9977027226484894E-5 24-25 6.663603630197986E-5 1.6659009075494965E-5 0.0 0.003231847760646023 5.830653176423237E-5 26-27 9.16245499152223E-5 1.6659009075494965E-5 0.0 0.0049227371818087624 6.663603630197986E-5 28-29 9.995405445296979E-5 1.6659009075494965E-5 0.0 0.01326057122409399 7.496554083972733E-5 30-31 1.1661306352846475E-4 1.6659009075494965E-5 0.0 0.03824908483733644 8.329504537747482E-5 32-33 1.3327207260395972E-4 1.6659009075494965E-5 0.0 0.08467774313074089 9.16245499152223E-5 34-35 1.499310816794547E-4 1.6659009075494965E-5 0.0 0.14559140981528823 1.1661306352846475E-4 36-37 1.6659009075494963E-4 1.6659009075494965E-5 0.0 0.2364829633311888 1.1661306352846475E-4 38-39 1.6659009075494963E-4 1.6659009075494965E-5 0.0 0.372145603737482 1.1661306352846475E-4 40-41 1.6659009075494963E-4 1.6659009075494965E-5 0.0 0.5335214246518017 1.1661306352846475E-4 42-43 1.6659009075494963E-4 1.6659009075494965E-5 0.0 0.6828194639863876 1.1661306352846475E-4 44-45 1.6659009075494963E-4 1.6659009075494965E-5 0.0 0.8595715502773891 1.1661306352846475E-4 46-47 1.6659009075494963E-4 1.6659009075494965E-5 0.0 1.058413482602497 1.1661306352846475E-4 48-49 1.6659009075494963E-4 1.6659009075494965E-5 0.0 1.2646853329752759 1.1661306352846475E-4 50-51 1.9157860436819208E-4 1.6659009075494965E-5 0.0 1.4831432484867788 1.1661306352846475E-4 52-53 2.1656711798143455E-4 1.6659009075494965E-5 0.0 1.7045581381091826 1.416015771417072E-4 54-55 2.332261270569295E-4 2.4988513613242447E-5 0.0 1.9319369529806134 1.6659009075494963E-4 56-57 2.332261270569295E-4 3.331801815098993E-5 0.0 2.19216733374892 1.6659009075494963E-4 58-59 2.332261270569295E-4 3.331801815098993E-5 0.0 2.4753704880323344 1.6659009075494963E-4 60-61 2.332261270569295E-4 3.331801815098993E-5 0.0 2.761613911472027 1.832490998304446E-4 62-63 2.332261270569295E-4 3.331801815098993E-5 0.0 3.051030876140601 1.9157860436819208E-4 64-65 2.332261270569295E-4 3.331801815098993E-5 0.0 3.356498796053414 1.9990810890593958E-4 66-67 2.332261270569295E-4 5.830653176423237E-5 0.0 3.6852560106537693 1.9990810890593958E-4 68-69 2.332261270569295E-4 6.663603630197986E-5 0.0 4.047048040250831 1.9990810890593958E-4 70-71 2.332261270569295E-4 7.496554083972733E-5 0.0 4.448638442529251 1.9990810890593958E-4 72-73 2.332261270569295E-4 8.329504537747482E-5 0.0 4.839692021567419 2.1656711798143455E-4 74-75 2.332261270569295E-4 8.329504537747482E-5 0.0 5.2281967722170375 2.1656711798143455E-4 76-77 2.4155563159467696E-4 8.329504537747482E-5 0.0 5.64630458199381 2.1656711798143455E-4 78-79 2.4988513613242444E-4 8.329504537747482E-5 0.0 6.083703524280006 2.1656711798143455E-4 80-81 2.4988513613242444E-4 8.329504537747482E-5 0.0 6.556719427969609 2.1656711798143455E-4 82-83 2.5821464067017196E-4 8.329504537747482E-5 0.0 7.050850625662404 2.332261270569295E-4 84-85 2.832031542834144E-4 8.329504537747482E-5 0.0 7.5451400839414156 2.332261270569295E-4 86-87 2.832031542834144E-4 9.16245499152223E-5 0.0 8.056929831254234 2.332261270569295E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 1380 0.0 21.228338 1 GTATTAC 1045 0.0 20.023998 1 GTATAAG 2160 0.0 19.59035 1 CGTTAAC 485 0.0 19.17533 1 TAGTACT 2025 0.0 18.370132 4 ATGGGTA 3495 0.0 18.22723 5 GTACATG 34310 0.0 17.374882 1 TACATGG 33485 0.0 17.316633 2 GTATCAA 23255 0.0 16.856438 1 GTCCTAC 1810 0.0 16.698956 1 GTATATA 1590 0.0 16.669872 1 ATAGGAC 4760 0.0 16.606928 3 CTAGTAC 2050 0.0 16.558321 3 CATGGGT 4890 0.0 16.545797 4 ACATGGG 32100 0.0 16.397917 3 CATGGGG 12195 0.0 16.395851 4 GTACATA 1675 0.0 15.546326 1 GGGTACC 4015 0.0 15.5191 7 GTATTAG 3225 0.0 15.283776 1 GTATATG 1905 0.0 15.133915 1 >>END_MODULE