FastQCFastQC Report
Fri 27 May 2016
ERR765434_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765434_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6002758
Sequences flagged as poor quality0
Sequence length99
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT99080.1650574619200041No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA99020.16495750786555113No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95570.15921014973450537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63610.10596795672922346No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA60770.1012367981517829No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT60110.10013730355280023No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA267800.025.5580841
ATCAACG323100.021.0130353
CAACGCA324200.020.984775
AACGCAG336500.020.4512846
TCAACGC335750.020.262884
AGTACTT163600.019.826612-13
TATCAAC355500.019.3072152
ACGCAGA365500.018.6377777
CGCAGAG369250.018.3855368
GTACTAG15250.018.3065971
GGTATCA195800.018.1079161
TATCACG5500.017.755362
TACTTTT185150.017.60684814-15
GAGTACT154700.017.42009212-13
GCAGAGT395450.017.1562399
TAGTACT21600.017.0077254
GTATAAG18650.016.466121
AGAGTAC374450.016.1882910-11
CTAGTAC21800.015.9984393
GTACTTT172000.015.61388114-15
Produced by FastQC (version 0.11.5)