##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765431_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5945731
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.502098732687365	34.0	31.0	34.0	31.0	34.0
2	32.54261402004228	34.0	31.0	34.0	31.0	34.0
3	32.56412861597674	34.0	31.0	34.0	31.0	34.0
4	36.02851827639024	37.0	37.0	37.0	35.0	37.0
5	36.05993056194436	37.0	37.0	37.0	35.0	37.0
6	36.16595739026875	37.0	37.0	37.0	35.0	37.0
7	36.13188655860819	37.0	37.0	37.0	35.0	37.0
8	36.16540119288948	37.0	37.0	37.0	35.0	37.0
9	37.901362843357695	39.0	38.0	39.0	35.0	39.0
10-11	37.849904914971766	39.0	38.0	39.0	35.0	39.0
12-13	37.833010692882	39.0	38.0	39.0	35.0	39.0
14-15	39.262210735736275	41.0	39.0	41.0	36.0	41.0
16-17	39.22355030861638	41.0	39.0	41.0	36.0	41.0
18-19	39.21958645623221	41.0	39.0	41.0	36.0	41.0
20-21	39.20281290895939	41.0	39.0	41.0	36.0	41.0
22-23	39.11493658223017	41.0	39.0	41.0	36.0	41.0
24-25	39.04336649269871	41.0	39.0	41.0	36.0	41.0
26-27	38.918207197735654	41.0	39.0	41.0	35.0	41.0
28-29	38.77744385004972	40.0	38.5	41.0	35.0	41.0
30-31	38.54329005129899	40.0	38.0	41.0	35.0	41.0
32-33	38.26741043279624	40.0	38.0	41.0	35.0	41.0
34-35	38.12001106339994	40.0	38.0	41.0	34.0	41.0
36-37	37.964439528125304	40.0	38.0	41.0	34.0	41.0
38-39	37.76576538696419	40.0	37.5	41.0	33.5	41.0
40-41	37.49743059347959	40.0	37.0	41.0	33.0	41.0
42-43	37.20909178366798	40.0	37.0	41.0	33.0	41.0
44-45	36.81260790304842	40.0	36.0	41.0	31.5	41.0
46-47	36.335131962747724	39.5	36.0	41.0	30.5	41.0
48-49	35.888526742969034	39.0	35.0	41.0	29.5	41.0
50-51	34.62326953573917	38.0	34.0	40.0	27.0	40.5
52-53	34.5486967876616	38.0	34.5	39.5	26.5	40.0
54-55	35.34032308222488	39.0	35.0	40.5	28.0	41.0
56-57	35.3335868205272	39.0	35.0	41.0	28.0	41.0
58-59	35.1156249752974	38.5	35.0	41.0	28.0	41.0
60-61	34.75992195072398	38.0	35.0	40.0	27.0	41.0
62-63	34.303758108128335	37.0	35.0	40.0	26.0	41.0
64-65	33.99552149264741	37.0	35.0	39.5	26.0	41.0
66-67	33.689834521608866	36.0	34.0	39.0	25.0	41.0
68-69	33.44045341102717	36.0	34.0	39.0	24.5	41.0
70-71	33.05622386885649	35.0	34.0	38.5	24.0	40.5
72-73	32.658386832502174	35.0	34.0	37.0	22.5	39.5
74-75	32.26206979427761	35.0	34.0	37.0	20.0	39.0
76-77	31.887278788764576	35.0	34.0	36.0	19.5	39.0
78-79	31.592151663100804	35.0	33.5	36.0	17.5	37.0
80-81	31.308716539648366	35.0	33.0	35.5	14.0	37.0
82-83	31.061773649026502	35.0	33.0	35.0	8.5	36.5
84-85	30.819161680876583	35.0	33.0	35.0	4.0	36.0
86-87	30.566055376538227	35.0	33.0	35.0	2.0	36.0
88-89	30.35781176444074	35.0	33.0	35.0	2.0	36.0
90-91	30.13541253379946	35.0	32.5	35.0	2.0	35.0
92-93	29.869473324642506	35.0	32.0	35.0	2.0	35.0
94-95	29.601144249546437	35.0	32.0	35.0	2.0	35.0
96-97	29.347042441038788	35.0	31.5	35.0	2.0	35.0
98-99	29.048384546828643	35.0	31.0	35.0	2.0	35.0
100	28.520072636989465	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3989420673981172
1101	2	0.4306793804055786
1101	3	0.4434886407107541
1101	4	0.328071516805565
1101	5	0.3613034713969796
1101	6	0.3638254890615613
1101	7	0.3852426710285002
1101	8	0.31814874283766414
1101	9	0.41336525697918347
1101	10-11	0.41461809167620345
1101	12-13	0.40824533124322926
1101	14-15	0.5906593343625701
1101	16-17	0.5568938820806011
1101	18-19	0.5389267415422623
1101	20-21	0.5311773573733589
1101	22-23	0.4727797727630261
1101	24-25	0.5097668605798304
1101	26-27	0.5644152998905909
1101	28-29	0.5707934053819415
1101	30-31	0.5817364088758197
1101	32-33	0.5468576486190031
1101	34-35	0.6500512671937244
1101	36-37	0.7117093812591335
1101	38-39	0.6805309268522848
1101	40-41	0.7304270034211129
1101	42-43	0.7681584023314656
1101	44-45	0.8279177023944229
1101	46-47	0.8573736599967816
1101	48-49	0.9538457073311335
1101	50-51	0.8714501723126915
1101	52-53	0.9483601582873504
1101	54-55	1.0832734977241714
1101	56-57	1.1080792678625642
1101	58-59	1.1499014702183388
1101	60-61	1.1157287800259965
1101	62-63	1.1569170791609764
1101	64-65	1.0610485505873228
1101	66-67	1.047157608690128
1101	68-69	0.9938689242430669
1101	70-71	0.9903251156310588
1101	72-73	0.9742746460333365
1101	74-75	0.9769359055788911
1101	76-77	0.8802997527348886
1101	78-79	0.899204346557724
1101	80-81	1.0177326229231767
1101	82-83	1.0141396215086225
1101	84-85	1.022917313740411
1101	86-87	1.0032923795105795
1101	88-89	0.9656847959063164
1101	90-91	0.9817968558753591
1101	92-93	0.9946362891156468
1101	94-95	0.9851682880657364
1101	96-97	0.9531280824369581
1101	98-99	0.9307897979851063
1101	100	1.0179437970275558
1102	1	0.3683464556466234
1102	2	0.43171182198080515
1102	3	0.4676297086602048
1102	4	0.34461784838178033
1102	5	0.35860950871269637
1102	6	0.31344842442253196
1102	7	0.35965336809970694
1102	8	0.3292410111910442
1102	9	0.42173047494265603
1102	10-11	0.39365149050392034
1102	12-13	0.4977888876585581
1102	14-15	0.6312686341335763
1102	16-17	0.6439657568397692
1102	18-19	0.6134697180777096
1102	20-21	0.6237516311977416
1102	22-23	0.5958645917631387
1102	24-25	0.5753036152745636
1102	26-27	0.6062417464993572
1102	28-29	0.6528157244477768
1102	30-31	0.7257237004927575
1102	32-33	0.7818362809467629
1102	34-35	0.6763339472182537
1102	36-37	0.7287527420025626
1102	38-39	0.7118813777088917
1102	40-41	0.8192137489895046
1102	42-43	0.8667798205403869
1102	44-45	0.9398327729767786
1102	46-47	0.9443907766687616
1102	48-49	1.0363787263074826
1102	50-51	1.029594213423465
1102	52-53	1.050074732396375
1102	54-55	1.1662814060238276
1102	56-57	1.2016058178732294
1102	58-59	1.21359967894535
1102	60-61	1.2515369861074959
1102	62-63	1.2516341481055449
1102	64-65	1.222352073237417
1102	66-67	1.1711514382273762
1102	68-69	1.0697133630523012
1102	70-71	1.0445393964200136
1102	72-73	1.0441925171651008
1102	74-75	0.9800018889988351
1102	76-77	0.9391512886128517
1102	78-79	0.9646773482668749
1102	80-81	0.937215925912966
1102	82-83	0.9405174484042611
1102	84-85	0.9985143421414548
1102	86-87	1.0454294224026128
1102	88-89	0.9751776436288075
1102	90-91	1.015861475075301
1102	92-93	1.1062402939523857
1102	94-95	0.9994064235506102
1102	96-97	1.2262373848369528
1102	98-99	1.2718320284134457
1102	100	1.2505287347828578
1103	1	0.30007139988879317
1103	2	0.3619453087368072
1103	3	0.4010011326358409
1103	4	0.2893326109804235
1103	5	0.35612130800118535
1103	6	0.2964105359534557
1103	7	0.3312712575893002
1103	8	0.34182551722097543
1103	9	0.4085752453394136
1103	10-11	0.4008258013775503
1103	12-13	0.44677094946080587
1103	14-15	0.5890585709670546
1103	16-17	0.5915228118820934
1103	18-19	0.5751802300914193
1103	20-21	0.5540585653531167
1103	22-23	0.5997148998499711
1103	24-25	0.5735199230071899
1103	26-27	0.5998327409188349
1103	28-29	0.5979913840538345
1103	30-31	0.6489305839187125
1103	32-33	0.6965793101048021
1103	34-35	0.730139494265508
1103	36-37	0.7415659827046497
1103	38-39	0.7546912819166636
1103	40-41	0.7752870096695688
1103	42-43	0.7605323002956226
1103	44-45	0.8439106637582867
1103	46-47	0.7653300277363968
1103	48-49	0.8626173742053496
1103	50-51	0.8116711150591485
1103	52-53	0.8429444086878704
1103	54-55	1.0197007685180708
1103	56-57	0.9933091699581098
1103	58-59	1.0398366130116727
1103	60-61	1.057445492804014
1103	62-63	1.0894017938211533
1103	64-65	1.0622142507914916
1103	66-67	0.9880019642711844
1103	68-69	0.9627979255503263
1103	70-71	0.9791597088787043
1103	72-73	0.9004432000474623
1103	74-75	0.9303871547967901
1103	76-77	0.9773038997641912
1103	78-79	0.9729037573579689
1103	80-81	0.9938481288996002
1103	82-83	1.0073460303369295
1103	84-85	1.0227254637138081
1103	86-87	1.03845955219726
1103	88-89	1.0561270843967385
1103	90-91	1.1009835126026282
1103	92-93	1.070556448309457
1103	94-95	1.0891700822933217
1103	96-97	1.1712599303792892
1103	98-99	1.2429156265013575
1103	100	1.2128864213915485
1104	1	0.27404997383322893
1104	2	0.3213408623092988
1104	3	0.3024351083843442
1104	4	0.2337864984242728
1104	5	0.28834982105799156
1104	6	0.22535172393037328
1104	7	0.2986881899135696
1104	8	0.2785285843597478
1104	9	0.3292358426327411
1104	10-11	0.3425747755567272
1104	12-13	0.3215003111090766
1104	14-15	0.39377713904326583
1104	16-17	0.3897389160066709
1104	18-19	0.4142986985739938
1104	20-21	0.3500125419709903
1104	22-23	0.34668066597624403
1104	24-25	0.3602431741576595
1104	26-27	0.3912862102995476
1104	28-29	0.3856976908623153
1104	30-31	0.3709359989230876
1104	32-33	0.39571285187307836
1104	34-35	0.3970406555733206
1104	36-37	0.3716940640824973
1104	38-39	0.38215582485667454
1104	40-41	0.34952372481834715
1104	42-43	0.3711812602197284
1104	44-45	0.5372624370427701
1104	46-47	0.4612815272981976
1104	48-49	0.4642514001981368
1104	50-51	0.33905087875758255
1104	52-53	0.35262739665034104
1104	54-55	0.5728123824271663
1104	56-57	0.5513707249612239
1104	58-59	0.5039701356027422
1104	60-61	0.5535708177777892
1104	62-63	0.55262415930423
1104	64-65	0.5671154977575057
1104	66-67	0.5826512674673197
1104	68-69	0.5729764685555381
1104	70-71	0.5666207232123952
1104	72-73	0.5691574385492899
1104	74-75	0.4987799353449631
1104	76-77	0.4814580186769497
1104	78-79	0.44222650465925284
1104	80-81	0.47603306175060567
1104	82-83	0.49159310437437753
1104	84-85	0.42578147969993196
1104	86-87	0.39130073550771627
1104	88-89	0.4305423553355361
1104	90-91	0.37714699382846106
1104	92-93	0.40450039460816
1104	94-95	0.5337402475433279
1104	96-97	0.5972466297068735
1104	98-99	0.5658430497174152
1104	100	0.6020901964889234
1105	1	0.22457335561333736
1105	2	0.27134463286243715
1105	3	0.2871627729327315
1105	4	0.25193315553887174
1105	5	0.2659741985541686
1105	6	0.21597566346230224
1105	7	0.2169859591963288
1105	8	0.19780800265041165
1105	9	0.29669640409710496
1105	10-11	0.2872158043956574
1105	12-13	0.31981603402562087
1105	14-15	0.38597376178782383
1105	16-17	0.35238071055793085
1105	18-19	0.37005488300625444
1105	20-21	0.34521676092990816
1105	22-23	0.30132286948749964
1105	24-25	0.37954427312888583
1105	26-27	0.30663823494156617
1105	28-29	0.3251197665808263
1105	30-31	0.353241880695947
1105	32-33	0.3604969006930432
1105	34-35	0.3531041400864936
1105	36-37	0.3635488716827737
1105	38-39	0.3431330652178346
1105	40-41	0.390700122895943
1105	42-43	0.5125170366781333
1105	44-45	0.5507157706468107
1105	46-47	0.4361548512148161
1105	48-49	0.4254036812377606
1105	50-51	0.43882167263304694
1105	52-53	0.46114899176834
1105	54-55	0.5227454551089323
1105	56-57	0.5346538325931149
1105	58-59	0.5326712235523416
1105	60-61	0.5670672952110394
1105	62-63	0.6122474810455287
1105	64-65	0.5537697699392297
1105	66-67	0.4559093444272122
1105	68-69	0.4402292870440121
1105	70-71	0.37877742160004857
1105	72-73	0.40004871029885436
1105	74-75	0.4923191650499632
1105	76-77	0.5119179246451502
1105	78-79	0.4959034465666292
1105	80-81	0.4975534212873498
1105	82-83	0.44780335041746966
1105	84-85	0.458641560273751
1105	86-87	0.4237040715771201
1105	88-89	0.48241777045893386
1105	90-91	0.5124240714757526
1105	92-93	0.5261542462281916
1105	94-95	0.547409807867826
1105	96-97	0.55009999579568
1105	98-99	0.5573909923005935
1105	100	0.6232474952646534
1106	1	0.07850462957348725
1106	2	0.16432448459823235
1106	3	0.15840383890613907
1106	4	0.14645618386355608
1106	5	0.2144159432944619
1106	6	0.16945751746696658
1106	7	0.17315221504077272
1106	8	0.1787718367847546
1106	9	0.16905706996571723
1106	10-11	0.23205020899011686
1106	12-13	0.2317002386267717
1106	14-15	0.2678402003486582
1106	16-17	0.2510392927671816
1106	18-19	0.24448426007648294
1106	20-21	0.24933361588875158
1106	22-23	0.2881607767301162
1106	24-25	0.2822263393203315
1106	26-27	0.30478999520292405
1106	28-29	0.32599769556969704
1106	30-31	0.26500956392377617
1106	32-33	0.27862236979770216
1106	34-35	0.3286007435729559
1106	36-37	0.3712952554465474
1106	38-39	0.2974551206213363
1106	40-41	0.28434533214461766
1106	42-43	0.31137963089922494
1106	44-45	0.3624891143870528
1106	46-47	0.238892038706787
1106	48-49	0.3155028542396465
1106	50-51	0.2497709859043553
1106	52-53	0.36075752336959965
1106	54-55	0.4040364174001638
1106	56-57	0.42098056259065686
1106	58-59	0.4223059322756981
1106	60-61	0.4099985036356344
1106	62-63	0.4347814039331368
1106	64-65	0.43611842370588505
1106	66-67	0.4354857622378816
1106	68-69	0.4139000592766635
1106	70-71	0.4234498833307967
1106	72-73	0.39619379143034905
1106	74-75	0.44424447431384095
1106	76-77	0.4206231195254624
1106	78-79	0.3539035710429488
1106	80-81	0.42346474661252387
1106	82-83	0.4523198897615366
1106	84-85	0.44246800621523974
1106	86-87	0.41305859867496864
1106	88-89	0.4185002259892201
1106	90-91	0.43666830719872607
1106	92-93	0.45711962601216527
1106	94-95	0.393155403831166
1106	96-97	0.36575801731194346
1106	98-99	0.40155965468716204
1106	100	0.5162321007317452
1107	1	0.061240647360151
1107	2	0.1673035236814897
1107	3	0.1840809015106757
1107	4	0.14631771344313194
1107	5	0.17668135973210042
1107	6	0.08730348169677882
1107	7	0.15662660012390006
1107	8	0.12519929064456647
1107	9	0.24049418555595992
1107	10-11	0.20436260367526415
1107	12-13	0.23362516333696703
1107	14-15	0.25240295231537146
1107	16-17	0.2501053011519403
1107	18-19	0.24023655048742398
1107	20-21	0.23137603361362835
1107	22-23	0.22904082409310433
1107	24-25	0.24247530515339122
1107	26-27	0.23958933244605163
1107	28-29	0.2562788213238605
1107	30-31	0.2729116048237685
1107	32-33	0.23351669907685846
1107	34-35	0.2989653785409061
1107	36-37	0.3591776332813197
1107	38-39	0.3482291702714875
1107	40-41	0.25803381480168497
1107	42-43	0.22057087522468777
1107	44-45	0.2511699155867362
1107	46-47	0.2550577500160571
1107	48-49	0.3493359042640023
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2316	64-65	-0.5280228900481347
2316	66-67	-0.44218192305728365
2316	68-69	-0.5085973621894695
2316	70-71	-0.5353708435564926
2316	72-73	-0.5175436183719526
2316	74-75	-0.47997063862312217
2316	76-77	-0.46081980442609094
2316	78-79	-0.3815011310841996
2316	80-81	-0.3512988885929538
2316	82-83	-0.26614403669058007
2316	84-85	-0.26723553230420194
2316	86-87	-0.34112497662268737
2316	88-89	-0.33751386073885215
2316	90-91	-0.2723840434958653
2316	92-93	-0.22891705238178872
2316	94-95	-0.1531326410740661
2316	96-97	-0.07365267579174173
2316	98-99	-0.14615288330870868
2316	100	-0.23522039404224415
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13581.0
3	2212.0
4	1993.0
5	2026.0
6	2632.0
7	2940.0
8	3665.0
9	4148.0
10	5789.0
11	9419.0
12	16343.0
13	24689.0
14	26272.0
15	33602.0
16	46903.0
17	67994.0
18	67935.0
19	37390.0
20	29887.0
21	25997.0
22	25237.0
23	28642.0
24	31863.0
25	33282.0
26	36200.0
27	41869.0
28	49223.0
29	59760.0
30	74173.0
31	96257.0
32	129780.0
33	181699.0
34	273991.0
35	457304.0
36	858249.0
37	1554874.0
38	1322727.0
39	264860.0
40	324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.84613368053597	17.79364128204787	13.26778890164951	24.092436135766647
2	14.98312702558387	21.70735667084975	37.0591981843748	26.250318119191583
3	19.20325286681589	27.058063422868972	29.228829816573064	24.50985389374207
4	13.395280416711602	18.05332843211466	34.440647575204764	34.11074357596897
5	13.643799213743849	36.46664736079322	33.44437302658021	16.445180398882734
6	31.222950274730316	33.9015140352511	18.163135365956546	16.712400324062042
7	25.435461786040904	32.1139740866932	22.20145212876148	20.249111998504418
8	27.63332256822848	30.908922835077355	21.243009276736498	20.214745319957665
9	27.83571167986223	14.400318816671145	19.570086166440166	38.19388333702646
10-11	24.922926821870405	25.586635341141122	26.09334551641831	23.397092320570163
12-13	27.318243766697737	23.564679237448164	26.391284996679197	22.7257919991749
14-15	25.675913164539843	22.90311744997781	25.286814003999368	26.134155381482984
16-17	22.997406904783276	26.122289555395056	27.358013344530395	23.522290195291273
18-19	24.16768484905034	25.05066350347316	29.191862277123743	21.589789370352758
20-21	24.17917820682507	24.555730743925157	28.451649483693963	22.813441565555806
22-23	23.067444692727737	25.33288540717726	26.63029358056072	24.969376319534284
24-25	23.019586680750503	26.237158472062532	26.156073368584483	24.587181478602485
26-27	23.907375683674516	24.765929384655035	26.647346071113944	24.67934886055651
28-29	23.14258515644886	24.28138204423319	28.854510302092862	23.721522497225088
30-31	24.550190096126624	24.468598748870882	27.886688889488088	23.094522265514406
32-33	22.043547111523118	25.496492379019557	27.96860304690922	24.491357462548105
34-35	21.909912801322246	26.090572828521896	28.458481384624307	23.541032985531547
36-37	23.587720188598542	25.775226974217425	27.15737232558245	23.47968051160158
38-39	24.234877822826604	24.63160690553167	26.839970928471114	24.293544343170616
40-41	23.170610911580113	25.660253949750167	26.976305748908047	24.192829389761673
42-43	24.957277126626323	25.013522879163187	26.524896066533575	23.504303927676915
44-45	24.691790913312268	24.80886723065861	26.87142200760382	23.627919848425307
46-47	23.534402611954658	25.123879480866094	28.23384451328439	23.107873393894856
48-49	24.17029174710268	25.83642851707659	27.228892535012577	22.76438720080815
50-51	22.88773374081068	26.40105504759869	26.164685167398343	24.54652604419229
52-53	22.53162374729589	26.692470563615732	24.822003701976186	25.953901987112193
54-55	23.404169210823085	26.264596137728656	24.59862488082746	25.73260977062079
56-57	24.139863286943257	24.78238032053651	25.169452360131327	25.9083040323889
58-59	23.698384941587488	24.599543528360453	26.94746670361448	24.754604826437575
60-61	23.802227283503363	25.230183514782873	26.211107601709323	24.75648160000444
62-63	22.041633929965414	25.49201420874534	26.79522598949704	25.671125871792206
64-65	21.9223320649162	25.593892945234952	26.893577198928252	25.590197790920595
66-67	23.729473649213666	25.105740244841137	25.92717724826642	25.23760885767878
68-69	24.087123989142786	24.265799552949193	25.853049574431502	25.79402688347652
70-71	22.967132466502527	24.91730393702378	26.15618529339833	25.959378303075358
72-73	24.42001553648231	24.296102616570987	26.1053912435712	25.178490603375504
74-75	24.59626140385047	23.860213071809973	26.320705220785012	25.222820303554546
76-77	23.241063326269337	24.772411057843783	27.477396954001353	24.509128661885523
78-79	23.63736834610514	24.668595259226098	27.098990726806214	24.595045667862543
80-81	22.720764337793042	24.886829653959264	26.743398154515617	25.649007853732076
82-83	22.7828639841582	25.222531117816633	25.461993493547432	26.532611404477734
84-85	23.276522943046302	25.14997311419331	25.164483449333204	26.40902049342719
86-87	23.64418693244322	23.860797304389287	25.8255017232055	26.669514039961996
88-89	23.771591506925848	23.290560519549512	26.704693932359895	26.233154041164745
90-91	24.28760625543454	23.935659937461153	26.00565145783168	25.77108234927263
92-93	23.279280665425556	24.20614913627565	26.380345429138448	26.13422476916035
94-95	22.84177602185574	24.375791312235272	26.82537510562078	25.957057560288206
96-97	24.39138054248418	23.90634265454097	25.716279578693463	25.985997224281387
98-99	24.72876496142153	23.377258850262358	25.49106798123928	26.402908207076837
100	24.582911217418868	23.601261739773598	26.080011909829626	25.73581513297791
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1220.0
1	1536.0
2	1316.0
3	748.0
4	600.5
5	574.5
6	833.0
7	1440.0
8	2196.0
9	2356.5
10	1927.0
11	1725.0
12	1928.0
13	2221.0
14	2574.5
15	3008.5
16	3432.0
17	4018.0
18	4720.5
19	5382.5
20	6208.0
21	7329.5
22	8756.0
23	10589.0
24	12745.5
25	15440.0
26	19266.5
27	23820.0
28	28185.0
29	34172.0
30	41219.5
31	47832.0
32	55304.0
33	63066.0
34	70643.5
35	76199.0
36	80937.5
37	87976.0
38	93287.0
39	97953.0
40	101085.0
41	103294.5
42	108268.0
43	119315.5
44	135376.5
45	151964.5
46	175195.0
47	203983.0
48	281361.0
49	378169.5
50	493469.5
51	467873.0
52	315946.5
53	262875.5
54	237699.5
55	208659.0
56	189131.5
57	169946.5
58	156248.5
59	142632.5
60	122108.5
61	101708.0
62	83402.0
63	65433.0
64	49053.0
65	39237.0
66	31393.5
67	23799.0
68	18318.0
69	14956.5
70	12733.0
71	10720.0
72	8800.5
73	8518.0
74	7178.0
75	5632.5
76	4384.5
77	3021.0
78	2197.0
79	1564.5
80	1181.0
81	964.0
82	772.5
83	581.0
84	398.0
85	285.0
86	210.0
87	135.0
88	94.0
89	74.5
90	63.0
91	54.5
92	42.5
93	35.0
94	34.0
95	27.0
96	19.5
97	12.5
98	10.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10851483190208236
2	0.34106823870773834
3	0.35092404954075457
4	0.3477116606856247
5	0.2795282867657484
6	0.12381993063594703
7	0.13860364688547128
8	0.11418276407055752
9	0.18981686187955696
10-11	0.18960662700683903
12-13	0.20044633704417503
14-15	0.13524829831689325
16-17	0.12223896439310826
18-19	0.139116619974903
20-21	0.1398061903574178
22-23	0.1470046323992794
24-25	0.15768456393334984
26-27	0.15239505453576693
28-29	0.15994669116379467
30-31	0.19999222971910433
32-33	0.19597253895273767
34-35	0.11859769639763387
36-37	0.081705680933093
38-39	0.07607138634425271
40-41	0.07463337981486212
42-43	0.06641740098904575
44-45	0.08681018364268413
46-47	0.1312286075505266
48-49	0.20492854453052117
50-51	0.1630665766749286
52-53	0.09974383301229067
54-55	0.05367716770233971
56-57	0.06631648825014115
58-59	0.0979189943170991
60-61	0.07648344669477984
62-63	0.08448919064787828
64-65	0.09282290100241669
66-67	0.08995529733854424
68-69	0.08065450656950339
70-71	0.11207200594846958
72-73	0.1361228753873998
74-75	0.1758404475412695
76-77	0.17607591059871358
78-79	0.13859523749056257
80-81	0.08068814414913827
82-83	0.1179165354100278
84-85	0.14436408239794232
86-87	0.15583449705343214
88-89	0.14443135755721206
90-91	0.16382342221671314
92-93	0.09001416310290526
94-95	0.317320107485522
96-97	0.3191785837603484
98-99	0.35518761275947397
100	0.35677698839722144
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5945731.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.94180616039483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7996913507887	29.518608085701718
2	12.100123949001015	9.182011147794956
3	3.8570228584371415	4.390272409531021
4	1.7880992077464446	2.713748541434847
5	0.9829209838980968	1.864689872102308
6	0.635054701215935	1.4457073424869478
7	0.43633691037063815	1.1588787331735828
8	0.34208347058062	1.038341178515601
9	0.2374974649538502	0.8109974500977736
>10	1.4936509383984027	10.795375523479079
>50	0.16076466795453082	4.227464455060965
>100	0.14127477485867318	11.033790163682891
>500	0.015118265678614443	3.860289120112693
>1k	0.008448440610519697	5.543795389323094
>5k	8.893095379493424E-4	2.4234355207997056
>10k+	0.0010227059686417437	9.99259506670292
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	84132	1.4149984249203336	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	80633	1.356149479349133	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	35675	0.6000103267369479	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	35205	0.592105495522754	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	28974	0.4873076161703246	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	28972	0.4872739785906897	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	28127	0.4730621011949582	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	27466	0.4619448811256345	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	27434	0.46140667985147665	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	24800	0.4171059874723562	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	23316	0.39214690338328456	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23022	0.3872021791769591	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	19689	0.3311451527154525	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	16606	0.2792928237083043	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15142	0.2546701154155814	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	13313	0.22390854883949507	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	13017	0.21893018705353473	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	12036	0.20243095424263224	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	11759	0.1977721494632031	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11166	0.1877986071014649	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	10618	0.1785819102815112	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	10610	0.17844735996297176	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	10582	0.17797643384808362	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	9379	0.15774342969771085	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	9212	0.1549346917981994	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	9029	0.15185685326160905	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	8888	0.1494854038973509	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	8105	0.13631629147030028	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	7909	0.13301980866608326	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	7776	0.1307829096203646	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	7695	0.12942058764515246	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	7628	0.1282937287273844	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	7594	0.12772188987359165	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	7105	0.11949750165286656	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	6796	0.11430049559927956	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	6776	0.11396411980293088	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	6398	0.10760661725194093	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	6332	0.10649657712399031	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.850066879917709E-4	0.0	0.0	0.0	1.681878981743372E-5
2	2.859194268963732E-4	1.681878981743372E-5	0.0	0.0	1.681878981743372E-5
3	3.531945861661081E-4	1.681878981743372E-5	0.0	0.0	1.681878981743372E-5
4	4.541073250707104E-4	3.363757963486744E-5	0.0	1.681878981743372E-5	1.681878981743372E-5
5	0.0018164293002828416	3.363757963486744E-5	0.0	3.363757963486744E-5	1.681878981743372E-5
6	0.0030946573264078042	3.363757963486744E-5	0.0	5.0456369452301156E-5	5.0456369452301156E-5
7	0.0034814894922087797	3.363757963486744E-5	0.0	5.0456369452301156E-5	5.0456369452301156E-5
8	0.0038010464987400204	3.363757963486744E-5	0.0	5.0456369452301156E-5	5.0456369452301156E-5
9	0.004120603505271261	3.363757963486744E-5	0.0	5.0456369452301156E-5	5.0456369452301156E-5
10-11	0.006878885035330391	3.363757963486744E-5	0.0	1.9341608290048776E-4	5.0456369452301156E-5
12-13	0.014573481376806316	3.363757963486744E-5	0.0	2.859194268963732E-4	1.0091273890460231E-4
14-15	0.02363880908840309	5.0456369452301156E-5	0.0	3.111476116225238E-4	1.1773152872203603E-4
16-17	0.027540768326047713	5.0456369452301156E-5	0.0	3.952415607096924E-4	1.5136910835690347E-4
18-19	0.02979448616158383	5.0456369452301156E-5	0.0	4.961542996142947E-4	1.9341608290048776E-4
20-21	0.03834684078374888	5.0456369452301156E-5	0.0	8.577582806891196E-4	2.1864426762663834E-4
22-23	0.0416517329828746	5.0456369452301156E-5	0.0	0.0023041742049884194	2.1864426762663834E-4
24-25	0.06256589812085343	5.0456369452301156E-5	0.0	0.005869757646284368	2.270536625353552E-4
26-27	0.12767143350413937	5.0456369452301156E-5	0.0	0.01108358248968882	2.522818472615058E-4
28-29	0.14269902220601638	5.0456369452301156E-5	0.0	0.03463829762900474	2.522818472615058E-4
30-31	0.1450956997550007	5.0456369452301156E-5	0.0	0.10173685960565657	2.6069124217022264E-4
32-33	0.14706349816364045	5.0456369452301156E-5	0.0	0.210546020329544	3.111476116225238E-4
34-35	0.1487790147250187	5.0456369452301156E-5	0.0	0.35112587501856374	3.3637579634867437E-4
36-37	0.15127660501290757	5.8865764361018015E-5	0.0	0.564489042642528	3.531945861661081E-4
38-39	0.15380783288043137	7.568455417845173E-5	0.0	0.8681270646115675	3.700133759835418E-4
40-41	0.15649883925122077	1.0091273890460231E-4	0.0	1.282844783929848	3.700133759835418E-4
42-43	0.16205744928588262	1.0091273890460231E-4	0.0	1.59354669762221	3.700133759835418E-4
44-45	0.169499763780097	1.0932213381331917E-4	0.0	1.921462642692715	3.952415607096924E-4
46-47	0.1730821660112104	1.1773152872203603E-4	0.0	2.3023409568983193	4.0365095561840925E-4
48-49	0.17572271601254746	1.1773152872203603E-4	0.0	2.6532397782543473	4.0365095561840925E-4
50-51	0.17943966856220034	1.1773152872203603E-4	0.0	3.0454371380070846	4.0365095561840925E-4
52-53	0.18083562811704734	1.5136910835690347E-4	0.0	3.496718906388466	4.541073250707104E-4
54-55	0.18498986920195348	1.5136910835690347E-4	0.0	3.8993439158280117	5.213824843404453E-4
56-57	0.19400474054409794	1.5136910835690347E-4	0.0	4.326684136904277	5.38201274157879E-4
58-59	0.19693961936724014	1.5136910835690347E-4	0.0	4.768152141427185	5.38201274157879E-4
60-61	0.19894946475042347	1.5136910835690347E-4	0.0	5.171189211217258	5.38201274157879E-4
62-63	0.2002192833816397	1.5136910835690347E-4	0.0	5.592441030379612	5.550200639753127E-4
64-65	0.20140500806376876	1.5136910835690347E-4	0.0	6.051989233956262	5.550200639753127E-4
66-67	0.20281778640843323	1.5977850326562033E-4	0.0	6.52289012065968	5.634294588840296E-4
68-69	0.2044239808359981	1.6818789817433719E-4	0.0	7.0732429704606545	5.718388537927464E-4
70-71	0.20682065838498243	1.6818789817433719E-4	0.0	7.72681273337122	5.718388537927464E-4
72-73	0.21254745631781863	1.6818789817433719E-4	0.0	8.269168248614005	5.97067038518897E-4
74-75	0.21792946905939742	1.6818789817433719E-4	0.0	8.790845061776256	6.054764334276139E-4
76-77	0.2197795359393151	1.6818789817433719E-4	0.0	9.369823491846503	6.391140130624813E-4
78-79	0.22104094517562264	1.6818789817433719E-4	0.0	9.93982068815424	6.55932802879915E-4
80-81	0.22315170329771056	1.6818789817433719E-4	0.0	10.570592581467274	6.727515926973487E-4
82-83	0.22407673673766942	1.6818789817433719E-4	0.0	11.301217629926413	6.895703825147825E-4
84-85	0.2278105080771397	1.6818789817433719E-4	0.0	11.98072196673546	6.895703825147825E-4
86-87	0.23495008435464032	1.850066879917709E-4	0.0	12.654482686821856	6.895703825147825E-4
88	0.23650582241275295	1.850066879917709E-4	0.0	13.19225508183939	6.895703825147825E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	6100	0.0	27.251003	1
TCTACAC	7925	0.0	24.537264	3
ATCTACA	7090	0.0	24.038553	2
TACACTC	8230	0.0	22.17838	5
TATACCG	880	0.0	21.383322	5
CTACACT	9785	0.0	20.878435	4
AGTACTT	26200	0.0	20.597322	12-13
GAGTACT	25225	0.0	19.930574	12-13
GTATAAG	1470	0.0	19.861498	1
CGTTAAC	485	0.0	18.44801	1
CACTCTT	10035	0.0	18.416084	7
TACTTTT	30150	0.0	17.981245	14-15
ATACCGA	945	0.0	17.910688	6
ATGGGTA	3930	0.0	17.716057	5
TACCGAC	905	0.0	17.66658	7
GTACTTT	28615	0.0	17.640625	14-15
ACACTCT	10435	0.0	17.616753	6
CATGGGT	6525	0.0	17.53052	4
GTACTAG	1560	0.0	17.50818	1
ACTCTTT	12005	0.0	16.841501	8
>>END_MODULE