##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765431_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5945731 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23099901425073 33.0 31.0 34.0 31.0 34.0 2 32.38520023862499 34.0 31.0 34.0 31.0 34.0 3 32.4250984445815 34.0 31.0 34.0 30.0 34.0 4 36.02409224366188 37.0 35.0 37.0 35.0 37.0 5 35.943671013707146 37.0 35.0 37.0 35.0 37.0 6 36.01570992700477 37.0 35.0 37.0 35.0 37.0 7 35.952103282170015 37.0 35.0 37.0 35.0 37.0 8 35.97131134926891 37.0 35.0 37.0 35.0 37.0 9 37.65261126007887 39.0 37.0 39.0 35.0 39.0 10-11 37.58617821761529 39.0 37.0 39.0 35.0 39.0 12-13 37.550209133241985 39.0 37.0 39.0 35.0 39.0 14-15 38.82022059188348 40.0 38.0 41.0 35.0 41.0 16-17 38.789491065102006 40.0 38.0 41.0 35.0 41.0 18-19 38.82774910267552 40.0 38.0 41.0 35.0 41.0 20-21 38.80855953624542 40.0 38.0 41.0 35.0 41.0 22-23 38.73438808449289 40.0 38.0 41.0 34.5 41.0 24-25 38.662842886770356 40.0 38.0 41.0 34.0 41.0 26-27 38.50242594224326 40.0 38.0 41.0 34.0 41.0 28-29 38.34911678648092 40.0 38.0 41.0 34.0 41.0 30-31 38.138623072587706 40.0 37.5 41.0 34.0 41.0 32-33 37.84274927674999 40.0 37.0 41.0 33.0 41.0 34-35 37.726446420128994 40.0 37.0 41.0 33.0 41.0 36-37 37.58551866877261 40.0 37.0 41.0 33.0 41.0 38-39 37.3896674773884 40.0 36.0 41.0 33.0 41.0 40-41 37.130677371714256 40.0 36.0 41.0 32.0 41.0 42-43 36.829968762461675 39.0 35.5 41.0 31.0 41.0 44-45 36.44557599393582 39.0 35.0 41.0 30.5 41.0 46-47 36.028686800664204 39.0 35.0 40.0 30.0 41.0 48-49 35.66610817071946 39.0 35.0 40.0 28.5 41.0 50-51 35.34580466220218 38.5 35.0 40.0 28.0 41.0 52-53 35.18640458170745 38.0 35.0 40.0 27.5 41.0 54-55 35.17145301393555 38.0 35.0 40.0 27.5 41.0 56-57 34.986943405276826 38.0 35.0 40.0 27.0 41.0 58-59 34.85448198043268 38.0 35.0 40.0 27.0 41.0 60-61 34.52733389721129 37.0 34.0 40.0 26.0 41.0 62-63 34.167446946388935 37.0 34.0 40.0 26.0 41.0 64-65 33.93533713516471 36.0 34.0 39.0 26.0 41.0 66-67 33.582764087376304 36.0 34.0 39.0 26.0 41.0 68-69 33.239638994767844 35.0 34.0 38.5 25.5 40.5 70-71 32.86294780238124 35.0 33.0 37.5 25.0 40.0 72-73 32.48730845710982 35.0 33.0 37.0 24.0 39.0 74-75 32.139580061728324 35.0 33.0 36.5 23.5 39.0 76-77 30.710677375078017 34.0 30.5 35.0 21.0 37.0 78-79 31.525859646189843 35.0 32.5 35.0 23.0 37.0 80-81 31.51617429379163 35.0 33.0 35.0 23.0 37.0 82-83 31.362612856182025 35.0 33.0 35.0 22.0 36.0 84-85 31.18155875198525 35.0 33.0 35.0 20.0 36.0 86-87 31.00297818720692 35.0 33.0 35.0 20.0 36.0 88-89 30.84384476862475 35.0 33.0 35.0 19.0 35.5 90-91 30.699594128964126 35.0 33.0 35.0 18.0 35.0 92-93 30.525411173159362 35.0 32.0 35.0 13.5 35.0 94-95 30.410480057035883 35.0 32.0 35.0 4.5 35.0 96-97 30.27843918603112 35.0 32.0 35.0 2.0 35.0 98-99 30.11570890105859 35.0 32.0 35.0 2.0 35.0 100 29.73250908929449 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39281512488489057 1101 2 0.411447987634574 1101 3 0.40996366916032656 1101 4 0.30498972051066175 1101 5 0.3409469527748641 1101 6 0.3597080072668817 1101 7 0.31127087599052317 1101 8 0.3587723087808996 1101 9 0.36113907154461344 1101 10-11 0.4312800378115753 1101 12-13 0.3929343576260109 1101 14-15 0.5359820032812834 1101 16-17 0.5358746998331227 1101 18-19 0.5220155989623763 1101 20-21 0.5090265893694763 1101 22-23 0.5184477613698846 1101 24-25 0.5339464668306206 1101 26-27 0.6222418224798858 1101 28-29 0.6160252647238096 1101 30-31 0.6797391113473026 1101 32-33 0.7005097353476444 1101 34-35 0.6458979782084882 1101 36-37 0.7354230456947377 1101 38-39 0.797346914803235 1101 40-41 0.7391780226824736 1101 42-43 0.9231306783137683 1101 44-45 0.9692180202897944 1101 46-47 0.992196135701576 1101 48-49 1.0008620696311041 1101 50-51 1.0041124397031567 1101 52-53 0.9502903826055729 1101 54-55 0.9964334694647761 1101 56-57 1.0420863138133782 1101 58-59 1.1152038010589322 1101 60-61 1.196998309066224 1101 62-63 1.1839313006723415 1101 64-65 1.1382043346073232 1101 66-67 1.1737650411929224 1101 68-69 1.1726257138385066 1101 70-71 1.1725694695425233 1101 72-73 1.2227017996358427 1101 74-75 1.2341426956043904 1101 76-77 1.2401733238095893 1101 78-79 1.1678332526238897 1101 80-81 1.241986628674102 1101 82-83 1.185315097212122 1101 84-85 1.2323139873279452 1101 86-87 1.2322068064149647 1101 88-89 1.3185877147419411 1101 90-91 1.2383985632202865 1101 92-93 1.3625408217456965 1101 94-95 1.3234270976288443 1101 96-97 1.3512213285752637 1101 98-99 1.3329504584701262 1101 100 1.3759482725663261 1102 1 0.42922459579558137 1102 2 0.37474726228897026 1102 3 0.41933364919954386 1102 4 0.31702699777331844 1102 5 0.3210542560915499 1102 6 0.331094803522177 1102 7 0.3848106866221812 1102 8 0.36450249097285337 1102 9 0.4255839695105408 1102 10-11 0.45854100223594685 1102 12-13 0.4736916259170556 1102 14-15 0.5744492215148682 1102 16-17 0.6255997691670743 1102 18-19 0.5749673828516606 1102 20-21 0.5558427633911336 1102 22-23 0.5836999467734145 1102 24-25 0.544941274802504 1102 26-27 0.6061517707398778 1102 28-29 0.6533746743505091 1102 30-31 0.7244095232467913 1102 32-33 0.7401279062292261 1102 34-35 0.8226725758942806 1102 36-37 0.8649566998348561 1102 38-39 0.8897596748046226 1102 40-41 0.9831374319921835 1102 42-43 0.9818236485922256 1102 44-45 1.0282643642598188 1102 46-47 1.0824872451665257 1102 48-49 1.0711984007130724 1102 50-51 1.0421021584028196 1102 52-53 1.1659210823478219 1102 54-55 1.232378292842526 1102 56-57 1.3509880446045912 1102 58-59 1.27409291645818 1102 60-61 1.322512220099128 1102 62-63 1.2267458152760753 1102 64-65 1.2789747388301649 1102 66-67 1.2857014253654455 1102 68-69 1.2400640726823013 1102 70-71 1.1557212924610312 1102 72-73 1.155271102417423 1102 74-75 1.1371062010293471 1102 76-77 1.18595140017376 1102 78-79 1.1542931137441634 1102 80-81 1.168524185435853 1102 82-83 1.1640560022243527 1102 84-85 1.1505513372708798 1102 86-87 1.1830124676350167 1102 88-89 1.2657063877604493 1102 90-91 1.3059512079758058 1102 92-93 1.30419934730946 1102 94-95 1.3739910082786722 1102 96-97 1.3296455155153168 1102 98-99 1.3418338314060492 1102 100 1.3722622420679507 1103 1 0.3890472251849104 1103 2 0.3478167494300024 1103 3 0.3854624366082433 1103 4 0.2550726941055501 1103 5 0.3221380690268063 1103 6 0.352566427206888 1103 7 0.35690745951625047 1103 8 0.3330678874630095 1103 9 0.47056526295087053 1103 10-11 0.38211718985759546 1103 12-13 0.3753856300226204 1103 14-15 0.5074144497456459 1103 16-17 0.5425786594268729 1103 18-19 0.49336196864224746 1103 20-21 0.5318784158930967 1103 22-23 0.5651758659913781 1103 24-25 0.5479578303445649 1103 26-27 0.5963432477743211 1103 28-29 0.6173513840511902 1103 30-31 0.6459409331499302 1103 32-33 0.5884915009983231 1103 34-35 0.6081039582489041 1103 36-37 0.706431963909786 1103 38-39 0.6984325741171205 1103 40-41 0.6870221474558704 1103 42-43 0.749243385797179 1103 44-45 0.7465334975812183 1103 46-47 0.8008062916231538 1103 48-49 0.774223002429224 1103 50-51 0.9189577432533085 1103 52-53 0.9608397944341647 1103 54-55 0.9945364611898313 1103 56-57 1.051915517232402 1103 58-59 0.9995393642755133 1103 60-61 1.0817507262260193 1103 62-63 1.0524603840880715 1103 64-65 1.0499918084510256 1103 66-67 1.0002665270059055 1103 68-69 1.0095808859705002 1103 70-71 0.8347321282581603 1103 72-73 0.9735261399160535 1103 74-75 0.9973797942704792 1103 76-77 1.0580758912011277 1103 78-79 1.116571734412961 1103 80-81 1.0789946148803402 1103 82-83 1.112854949076631 1103 84-85 1.0199676564243383 1103 86-87 1.0015752527406079 1103 88-89 1.0807264003657657 1103 90-91 1.1352318008583353 1103 92-93 1.1250084708581305 1103 94-95 1.1522635749170362 1103 96-97 1.182094230961468 1103 98-99 1.189310014801432 1103 100 1.2465046401826108 1104 1 0.25579803717987204 1104 2 0.21800104879389437 1104 3 0.2540307685171399 1104 4 0.17930795498270413 1104 5 0.26230498744759956 1104 6 0.1862436824625675 1104 7 0.19344982164970048 1104 8 0.1753284136728226 1104 9 0.20200875698707677 1104 10-11 0.2523359922153787 1104 12-13 0.28886888544321465 1104 14-15 0.42309432652568546 1104 16-17 0.39543229194169527 1104 18-19 0.365554665066405 1104 20-21 0.4016078478577967 1104 22-23 0.4333043526475535 1104 24-25 0.4265478356950041 1104 26-27 0.46447672050464917 1104 28-29 0.4732000490298347 1104 30-31 0.4650503499902072 1104 32-33 0.5973738245344364 1104 34-35 0.5255007065614663 1104 36-37 0.5058123003675163 1104 38-39 0.5870707334375425 1104 40-41 0.622115863276484 1104 42-43 0.6204295185021778 1104 44-45 0.6447793103975954 1104 46-47 0.677506015535279 1104 48-49 0.6358543787710644 1104 50-51 0.6471180033473942 1104 52-53 0.6630341784526479 1104 54-55 0.6881711622203497 1104 56-57 0.6263302927599881 1104 58-59 0.7374143186497335 1104 60-61 0.8226702062424209 1104 62-63 0.7855808610635933 1104 64-65 0.7544577647512867 1104 66-67 0.7796515554872485 1104 68-69 0.7219356425893508 1104 70-71 0.6662090705417896 1104 72-73 0.7038308274010703 1104 74-75 0.744791462451083 1104 76-77 0.6865953244251308 1104 78-79 0.7398616388273531 1104 80-81 0.6984183142021685 1104 82-83 0.6643533762259288 1104 84-85 0.7189817082040761 1104 86-87 0.7300324399427645 1104 88-89 0.7728047111349845 1104 90-91 0.7169004353196513 1104 92-93 0.7707545345763229 1104 94-95 0.7857232168018484 1104 96-97 0.8624004046801268 1104 98-99 0.8581187809590283 1104 100 0.8636531555151059 1105 1 0.22823790410835443 1105 2 0.19188590770269798 1105 3 0.23127069461429528 1105 4 0.1850278840963 1105 5 0.18195885066933215 1105 6 0.12790727951225023 1105 7 0.17406366770092063 1105 8 0.14931696279297313 1105 9 0.19678581578160248 1105 10-11 0.21092204928994818 1105 12-13 0.2577811823771796 1105 14-15 0.2590778028016487 1105 16-17 0.3039298135281143 1105 18-19 0.2757828705441483 1105 20-21 0.3134795069301788 1105 22-23 0.35108345334162294 1105 24-25 0.3503921611575507 1105 26-27 0.39081642703959574 1105 28-29 0.4143296777463661 1105 30-31 0.3520127923804992 1105 32-33 0.30879069688961636 1105 34-35 0.31075081025168316 1105 36-37 0.34677580721396595 1105 38-39 0.35452150596586307 1105 40-41 0.4178270041008503 1105 42-43 0.4950147576567616 1105 44-45 0.4243996882070249 1105 46-47 0.39069671561097863 1105 48-49 0.35521914208184313 1105 50-51 0.33119031675577304 1105 52-53 0.36503402182967193 1105 54-55 0.42978733731323615 1105 56-57 0.45771035871565857 1105 58-59 0.3692042872381265 1105 60-61 0.47962390384985554 1105 62-63 0.42945487238105784 1105 64-65 0.41666246518083483 1105 66-67 0.44579742658654453 1105 68-69 0.45978111952081946 1105 70-71 0.4157556615266884 1105 72-73 0.48727273332867327 1105 74-75 0.5414786689642739 1105 76-77 0.4994521130160159 1105 78-79 0.5642512442824774 1105 80-81 0.5640253587556039 1105 82-83 0.5951653938489159 1105 84-85 0.6260212584060909 1105 86-87 0.5677829279393549 1105 88-89 0.6192238458220451 1105 90-91 0.6448753292365872 1105 92-93 0.582024790422853 1105 94-95 0.6127339367744682 1105 96-97 0.7175965894604346 1105 98-99 0.6598582386374723 1105 100 0.7143433117610272 1106 1 0.22300750820084403 1106 2 0.11815622217576305 1106 3 0.19724947621097755 1106 4 0.12387470307380255 1106 5 0.19037174603105456 1106 6 0.13438908831525254 1106 7 0.1868235892945762 1106 8 0.15490658141320068 1106 9 0.19600557184924128 1106 10-11 0.2531210044857488 1106 12-13 0.22919931797200377 1106 14-15 0.3009379241207242 1106 16-17 0.30684402942417677 1106 18-19 0.28913219986272765 1106 20-21 0.27692863025831826 1106 22-23 0.2959487571895494 1106 24-25 0.2944540981546737 1106 26-27 0.3386456411365302 1106 28-29 0.34421106940258284 1106 30-31 0.2837890955245683 1106 32-33 0.26557409971764656 1106 34-35 0.3113013637797266 1106 36-37 0.3447615990530224 1106 38-39 0.3705261514329976 1106 40-41 0.4293382880987835 1106 42-43 0.38475278447083383 1106 44-45 0.40601810839373087 1106 46-47 0.3964817180833293 1106 48-49 0.4439473994285237 1106 50-51 0.42367587660999106 1106 52-53 0.5401285228479438 1106 54-55 0.6094934935189258 1106 56-57 0.5036618352094919 1106 58-59 0.44396735975876567 1106 60-61 0.4431537306972544 1106 62-63 0.35054267173732256 1106 64-65 0.4240333179109257 1106 66-67 0.4795486028259148 1106 68-69 0.4538920295677187 1106 70-71 0.5073428485996132 1106 72-73 0.40437583420408174 1106 74-75 0.40690824346548027 1106 76-77 0.41623311424800136 1106 78-79 0.43854582696600986 1106 80-81 0.43746381641435406 1106 82-83 0.3670944181675502 1106 84-85 0.3458311696061145 1106 86-87 0.3354297059193563 1106 88-89 0.32615018345814306 1106 90-91 0.3998202470925243 1106 92-93 0.419383595329311 1106 94-95 0.4448497057942369 1106 96-97 0.48029399124401806 1106 98-99 0.4745615020740068 1106 100 0.517419030869366 1107 1 0.12629798757216548 1107 2 0.05927381999250514 1107 3 0.11414154785141051 1107 4 0.07049224509081142 1107 5 0.13375561208430042 1107 6 0.1700565195917747 1107 7 0.06418340472187367 1107 8 0.08024633113568314 1107 9 0.10329740825723377 1107 10-11 0.1858017575726052 1107 12-13 0.22115272616716197 1107 14-15 0.22525025784171504 1107 16-17 0.1942517126021599 1107 18-19 0.2116568446104239 1107 20-21 0.25461862926605505 1107 22-23 0.19317761756896346 1107 24-25 0.18425374831705454 1107 26-27 0.20430073646431168 1107 28-29 0.21145726022469802 1107 30-31 0.26734567335677895 1107 32-33 0.2801291066341278 1107 34-35 0.30439802253228976 1107 36-37 0.34746185190824264 1107 38-39 0.32181982494385863 1107 40-41 0.3495552031408522 1107 42-43 0.320631859089076 1107 44-45 0.24437666117790258 1107 46-47 0.30603630454361763 1107 48-49 0.27969678713834156 1107 50-51 0.350602314139401 1107 52-53 0.2943756549237406 1107 54-55 0.36985907985697963 1107 56-57 0.40017897355933485 1107 58-59 0.4515683502988921 1107 60-61 0.34862536479929673 1107 62-63 0.2755681480377348 1107 64-65 0.255097479952596 1107 66-67 0.2849147790240636 1107 68-69 0.24609759465541714 1107 70-71 0.32352392308919065 1107 72-73 0.25344512047443146 1107 74-75 0.32867463321874624 1107 76-77 0.2142090059503765 1107 78-79 0.33075196823934405 1107 80-81 0.26288518270026984 1107 82-83 0.32072083368241877 1107 84-85 0.34414922530191916 1107 86-87 0.42344218222372376 1107 88-89 0.4287920530419491 1107 90-91 0.4449143277196974 1107 92-93 0.33912047072979945 1107 94-95 0.43476437270065205 1107 96-97 0.4361254585899701 1107 98-99 0.35207214803307707 1107 100 0.4987144252369724 1108 1 0.14244241438481708 1108 2 0.10875490915677233 1108 3 0.10390924092546783 1108 4 0.14195322812922484 1108 5 0.1775455855619441 1108 6 0.13353216154531822 1108 7 0.12830172329698541 1108 8 0.11978672164088522 1108 9 0.16801220308981613 1108 10-11 0.16900870046960392 1108 12-13 0.14010595338977794 1108 14-15 0.16005382071363528 1108 16-17 0.1926597533408838 1108 18-19 0.17995921089793399 1108 20-21 0.21168271934828908 1108 22-23 0.18340414462887367 1108 24-25 0.2488888131176097 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-0.8327842518686701 2316 90-91 -0.887441120789827 2316 92-93 -0.9572594128481988 2316 94-95 -0.9342923828308116 2316 96-97 -0.8766716016663239 2316 98-99 -0.9032525517948713 2316 100 -0.9628867188678782 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18.0 3 31.0 4 56.0 5 136.0 6 330.0 7 835.0 8 1846.0 9 3266.0 10 5596.0 11 9923.0 12 15557.0 13 21661.0 14 25902.0 15 34944.0 16 46773.0 17 62870.0 18 61124.0 19 37426.0 20 25798.0 21 22635.0 22 23705.0 23 26661.0 24 30146.0 25 34402.0 26 41144.0 27 51860.0 28 65453.0 29 81867.0 30 102657.0 31 128396.0 32 164050.0 33 216138.0 34 309545.0 35 499105.0 36 921719.0 37 1536453.0 38 1173273.0 39 162371.0 40 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52058645101838 19.06029721156238 13.982839115997681 23.436277221421552 2 15.747118138767208 22.878480459246703 35.65798957227409 25.716411829711998 3 19.993916956701888 27.110539293078368 29.626860135256873 23.268683614962875 4 14.379051437913553 18.748868877219778 33.936391373527854 32.935688311338815 5 13.837861147771402 37.45978417119779 32.66696727450334 16.035387406527473 6 31.53629049144672 34.31319042183375 17.448418033039168 16.702101053680362 7 25.48441898901918 32.83972988350802 21.896987939750385 19.77886318772242 8 28.07932279479176 31.15342756004266 21.009107206498243 19.75814243866734 9 28.266970032784865 14.34780685503599 19.438703163664822 37.946519948514315 10-11 25.399217404298703 25.964404854478257 25.520808937309848 23.1155688039132 12-13 27.603110102990904 23.943182990721308 26.040776057464534 22.412930848823258 14-15 25.861267750607496 23.404926104728922 24.968960824204064 25.764845320459518 16-17 23.31131407000989 26.507445797410334 27.0351209941028 23.14611913847698 18-19 24.65831367076647 25.297663146886396 28.71961412314146 21.324409059205674 20-21 24.82938766420946 25.005670188532825 27.76319408931306 22.40174805794466 22-23 23.47209991792869 25.80403013099368 26.020843370788654 24.703026580288974 24-25 23.592395157160194 26.647627649295245 25.492798558954938 24.267178634589623 26-27 24.329316118773832 25.26787620674324 26.11331022476987 24.289497449713057 28-29 23.68995532196426 24.52557234324801 28.36388370423184 23.420588630555887 30-31 25.122067415541355 24.82265209283355 27.21324007089291 22.842040420732182 32-33 22.548399366496056 25.83144869582636 27.669528445236917 23.950623492440666 34-35 22.374117840972545 26.512553736855914 27.84428589689251 23.269042525279026 36-37 24.19662593700028 26.23756360374922 26.503714362242718 23.06209609700778 38-39 24.6736830278508 24.941062414677894 26.40620793814196 23.979046619329342 40-41 23.54862047306788 25.999442115909545 26.563011006563674 23.888926404458903 42-43 25.57867903935823 25.180466387814654 26.24599134111179 22.994863231715325 44-45 25.19289101478964 25.039267775196087 26.550583066965032 23.21725814304924 46-47 24.046105362975204 25.49994542181536 27.71502921740938 22.738919997800053 48-49 24.846158503665656 26.09227966961164 26.803815511299707 22.257746315423002 50-51 23.485805325005646 26.81469701894375 25.60915563580127 24.090342020249334 52-53 23.144045493131845 27.030494654886677 24.29356821993438 25.5318916320471 54-55 24.041159632138626 26.45294855550617 24.073994485191307 25.431897327163906 56-57 24.622466708970897 25.014038791139797 24.719341938184233 25.64415256170507 58-59 24.071316150000552 24.92588723554573 26.423771332207803 24.57902528224592 60-61 24.353256479127193 25.57109047713737 25.564268749489 24.511384294246433 62-63 22.309667116772534 26.011056187879166 26.31121927556504 25.368057419783263 64-65 22.13860489188294 26.139361114594234 26.112558603685898 25.609475389836927 66-67 23.863295591332186 25.84448575521312 25.121965905216847 25.170252748237846 68-69 24.18511919533635 24.86284276064837 25.177897179941706 25.77414086407358 70-71 23.01214857890959 25.465124805826584 25.549017141081077 25.973709474182755 72-73 24.281589440395983 24.891074229976315 25.549148940553874 25.278187389073825 74-75 24.311371867265088 24.446122106271627 25.77462675621871 25.467879270244577 76-77 22.95286083895607 25.299727481481842 26.74162173326352 25.005789946298563 78-79 23.356991533975773 25.287218483166175 26.368597670094157 24.987192312763888 80-81 22.30650865301508 25.34463802684649 26.033039503468956 26.31581381666947 82-83 22.187880556935415 25.73912718797769 24.79385540121008 27.279136853876818 84-85 22.661853352057136 25.47244202750899 24.509666980309145 27.356037640124732 86-87 22.790234646303244 24.232934307831375 25.18423628799567 27.79259475786971 88-89 22.787897227396105 23.728513671621567 26.072103695955846 27.411485405026482 90-91 23.250905571782994 24.33554821466504 25.46451707631628 26.94902913723569 92-93 21.84000559733844 24.718951548543707 25.798533154222987 27.642509699894863 94-95 21.177311543072236 24.952685778486607 26.27702145570123 27.592981222739922 96-97 22.563722717564445 24.45538564624879 25.28700051684228 27.69389111934448 98-99 22.77385236567211 23.852702047906305 25.159454741561632 28.21399084485995 100 22.51020152103661 24.391953690089714 25.61445432164048 27.483390467233193 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 631.0 1 391.5 2 221.5 3 293.5 4 268.0 5 316.5 6 531.0 7 1128.0 8 1793.5 9 1896.5 10 1500.0 11 1291.0 12 1467.5 13 1687.0 14 1986.0 15 2365.0 16 2758.5 17 3243.0 18 3801.0 19 4411.5 20 5212.0 21 6172.5 22 7438.5 23 8843.0 24 10522.5 25 12792.5 26 16106.0 27 20168.5 28 24194.0 29 30060.5 30 36803.5 31 43604.5 32 51599.5 33 59399.0 34 67372.5 35 74799.0 36 81695.5 37 90949.0 38 98078.0 39 102910.5 40 106055.0 41 107782.0 42 111674.5 43 122068.0 44 137877.0 45 153915.5 46 175774.5 47 205313.0 48 282806.0 49 378398.0 50 496049.0 51 476448.0 52 329349.0 53 267718.5 54 236675.0 55 210531.5 56 189382.0 57 169725.0 58 157193.0 59 143975.5 60 122205.5 61 100673.5 62 82245.5 63 64734.5 64 48746.0 65 38979.0 66 30632.0 67 22815.5 68 17517.0 69 14277.5 70 12248.5 71 10098.0 72 8372.5 73 8447.5 74 6972.0 75 5241.5 76 3992.5 77 2713.0 78 1859.0 79 1152.0 80 765.0 81 587.0 82 447.5 83 335.0 84 223.5 85 135.5 86 93.5 87 53.5 88 32.0 89 15.0 90 8.0 91 6.0 92 5.0 93 3.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.055569281556801006 3 0.022503540775726315 4 0.0052306436332218865 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.568455417845173E-5 12-13 1.934160829004878E-4 14-15 3.1955700653124065E-4 16-17 4.709261148881441E-4 18-19 0.0 20-21 0.0027162345555155456 22-23 0.004902677231781929 24-25 7.736643316019512E-4 26-27 4.6251671997942726E-4 28-29 0.0051129121044998505 30-31 0.0024134963388017386 32-33 0.0011689058923116434 34-35 0.0015305098733864684 36-37 0.0038430934732836043 38-39 0.004145831689997412 40-41 8.661676755978365E-4 42-43 0.00275828153005913 44-45 2.691006370789395E-4 46-47 0.002220080255901251 48-49 1.681878981743372E-5 50-51 0.0017407447461043899 52-53 0.006626603188068885 54-55 0.013934367363743837 56-57 0.025009540458523938 58-59 0.026565278516636558 60-61 0.02504317803815881 62-63 0.007787099685471812 64-65 0.006601375003342734 66-67 2.0182547780920462E-4 68-69 0.0015389192682951853 70-71 2.522818472615058E-4 72-73 8.998052552327038E-4 74-75 0.004826992677603477 76-77 0.0013455031853946975 78-79 0.0013959595548469986 80-81 0.0 82-83 0.0014548253192080167 84-85 2.5228184726150578E-5 86-87 6.307046181537644E-4 88-89 4.2046974543584296E-5 90-91 6.727515926973487E-4 92-93 8.073019112368185E-4 94-95 0.0018248386951915585 96-97 1.6818789817433719E-4 98-99 0.0 100 8.241207010542522E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5945731.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.36636396380697 #Duplication Level Percentage of deduplicated Percentage of total 1 77.32304268330977 28.89280955693534 2 12.385187517373454 9.255788490683507 3 3.906839166078587 4.379531226832458 4 1.7934212016973088 2.6805451745211886 5 1.0047692011224545 1.87722858343826 6 0.666818577769849 1.494995140486578 7 0.45577388412567904 1.1921428987606675 8 0.33611681334073235 1.004757054531582 9 0.25177507767740753 0.8467127270558803 >10 1.5422249626148887 10.963933410353613 >50 0.16395540739102338 4.276882960604686 >100 0.14400450173519613 11.036722914500793 >500 0.015859486599106846 4.015422886309824 >1k 0.008178252074291388 5.259872038688754 >5k 9.036742623525427E-4 2.4295540726499794 >10k+ 0.0011295928279406783 10.393100863646925 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 80979 1.361968780625965 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 75626 1.2719377987332425 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 37559 0.631696926752993 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 36510 0.6140540162345051 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 32840 0.5523290576045233 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 30488 0.5127712639539193 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 28031 0.47144749737248454 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 27074 0.4553519155172005 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 26212 0.44085411869457264 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 25873 0.4351525489464626 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 24724 0.4158277594462313 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 22248 0.3741844358582654 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19816 0.3332811390222666 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 17091 0.28744993676975966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14499 0.24385563356297146 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 13434 0.2259436224074046 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 13160 0.22133527399742772 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 12954 0.2178706032950364 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 11877 0.19975676666166026 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11861 0.19948766602458134 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 11223 0.1887572781210586 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 10654 0.17918738671493883 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 10442 0.1756218032736429 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 10264 0.1726280586861397 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 10138 0.17050889116914306 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 9683 0.1628563418022107 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 9287 0.15619610103450693 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 9085 0.15279870549138533 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 8178 0.13754406312697295 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 8161 0.13725814370007658 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 8066 0.13566035866742038 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 7735 0.13009333923784983 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 7489 0.12595591694276112 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 7295 0.12269307171817899 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 7260 0.1221044140745688 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 7219 0.121414843692054 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 7178 0.12072527330953924 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 7101 0.11943022649359683 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 6904 0.1161169248995624 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.003986053186731791 0.0 0.0 3.363757963486744E-5 1.681878981743372E-5 2 0.0063070461815376445 0.0 0.0 6.727515926973487E-5 1.681878981743372E-5 3 0.006609784398251451 0.0 0.0 6.727515926973487E-5 1.681878981743372E-5 4 0.007585274207662607 0.0 0.0 1.0091273890460231E-4 1.681878981743372E-5 5 0.02966834523795308 1.681878981743372E-5 0.0 5.213824843404453E-4 1.681878981743372E-5 6 0.06093447550856236 1.681878981743372E-5 0.0 9.418522297762882E-4 3.363757963486744E-5 7 0.06774608538462301 1.681878981743372E-5 0.0 0.0010595837584983243 3.363757963486744E-5 8 0.07788781564453555 1.681878981743372E-5 0.0 0.0011604964974029266 3.363757963486744E-5 9 0.08515353284566692 1.681878981743372E-5 0.0 0.0012277716566726615 5.0456369452301156E-5 10-11 0.15181480628706545 1.681878981743372E-5 0.0 0.00232940238971457 5.0456369452301156E-5 12-13 0.26359248341373 1.681878981743372E-5 0.0 0.00554179124484441 5.0456369452301156E-5 14-15 0.3964441041816389 3.363757963486744E-5 0.0 0.009460569272306468 6.727515926973487E-5 16-17 0.45941365325811073 5.8865764361018015E-5 0.0 0.011335864336950326 6.727515926973487E-5 18-19 0.489729521904035 7.568455417845173E-5 0.0 0.012353401120905067 8.409394908716859E-5 20-21 0.6018856218015918 8.409394908716859E-5 0.0 0.016347863702545574 1.261409236307529E-4 22-23 0.6329415171994831 8.409394908716859E-5 0.0 0.01906409825806112 1.429597134481866E-4 24-25 0.7758507742782175 8.409394908716859E-5 0.0 0.03442806275628682 1.7659729308305405E-4 26-27 1.0697843545225978 8.409394908716859E-5 0.0 0.08490125099840541 1.850066879917709E-4 28-29 1.1365633594927185 8.409394908716859E-5 0.0 0.11810154209801957 1.850066879917709E-4 30-31 1.1555013168271486 8.409394908716859E-5 0.0 0.1860578623553605 2.0182547780920462E-4 32-33 1.1719248650838727 8.409394908716859E-5 0.0 0.29522021766541406 2.3546305744407206E-4 34-35 1.1856574069698076 8.409394908716859E-5 0.0 0.4359093944882471 2.522818472615058E-4 36-37 1.2066640754517821 8.409394908716859E-5 0.0 0.6485325353602442 2.522818472615058E-4 38-39 1.2282678109722758 8.409394908716859E-5 0.0 0.9506989132202583 2.691006370789395E-4 40-41 1.2479037480841297 8.409394908716859E-5 0.0 1.3639870354040573 2.691006370789395E-4 42-43 1.2853676024024634 1.0932213381331917E-4 0.0 1.675101009446946 2.859194268963732E-4 44-45 1.3298028451001231 1.1773152872203603E-4 0.0 2.003975625537045 3.0273821671380693E-4 46-47 1.3480932790265823 1.1773152872203603E-4 0.0 2.3851398591695454 3.111476116225238E-4 48-49 1.3600598479816863 1.1773152872203603E-4 0.0 2.733372902339511 3.3637579634867437E-4 50-51 1.3818569995850805 1.1773152872203603E-4 0.0 3.1208105445739136 3.3637579634867437E-4 52-53 1.3893665892385645 1.3455031853946975E-4 0.0 3.5686444610427213 3.700133759835418E-4 54-55 1.4119878615430128 1.3455031853946975E-4 0.0 3.968385048028577 4.0365095561840925E-4 56-57 1.4571210840180964 1.3455031853946975E-4 0.0 4.396675530729527 4.120603505271261E-4 58-59 1.4718963908727118 1.3455031853946975E-4 0.0 4.837025085729577 4.2046974543584296E-4 60-61 1.4818447050497239 1.429597134481866E-4 0.0 5.240331256156729 4.372885352532767E-4 62-63 1.4893038383337558 1.5136910835690347E-4 0.0 5.662701524841941 4.372885352532767E-4 64-65 1.4954595154069366 1.5136910835690347E-4 0.0 6.122602923004758 4.372885352532767E-4 66-67 1.503910957290197 1.5136910835690347E-4 0.0 6.593301984230366 4.541073250707104E-4 68-69 1.5137835869130305 1.5136910835690347E-4 0.0 7.143511874317893 4.709261148881441E-4 70-71 1.5258426592121306 1.5136910835690347E-4 0.0 7.797275053311359 4.8774490470557784E-4 72-73 1.5544430112966765 1.5136910835690347E-4 0.0 8.341539501198422 5.213824843404453E-4 74-75 1.5832535982539406 1.5136910835690347E-4 0.0 8.865932548916188 5.38201274157879E-4 76-77 1.5923189259655373 1.5136910835690347E-4 8.40939490871686E-6 9.44652558280891 5.550200639753127E-4 78-79 1.5994080458735858 1.5136910835690347E-4 1.681878981743372E-5 10.018381255391475 5.718388537927464E-4 80-81 1.6137797017725828 1.5136910835690347E-4 1.681878981743372E-5 10.65096957800479 5.802482487014633E-4 82-83 1.618850566902539 1.5136910835690347E-4 1.681878981743372E-5 11.382401928375167 5.886576436101802E-4 84-85 1.637527832994799 1.5136910835690347E-4 1.681878981743372E-5 12.065101835249525 5.886576436101802E-4 86-87 1.6687519162908648 1.5136910835690347E-4 1.681878981743372E-5 12.743109299764823 5.97067038518897E-4 88 1.6764801502119757 1.5136910835690347E-4 1.681878981743372E-5 13.284220224561118 6.054764334276139E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATC 1940 0.0 27.620457 3 CGATCTC 1975 0.0 23.555765 5 CGCGCCA 1550 0.0 23.36316 1 GATCTAC 2485 0.0 21.95352 1 CCGATCT 2560 0.0 21.477972 4 AGTACTT 24300 0.0 19.996347 12-13 TCTACAC 3440 0.0 19.949047 3 TCTCGCG 980 0.0 19.660484 8 ATCTACA 3125 0.0 19.564371 2 GAGTACT 23230 0.0 18.864098 12-13 GTATAAG 1480 0.0 18.112804 1 TACACTC 3305 0.0 18.057655 5 TACTTTT 27425 0.0 17.752464 14-15 GTACTAG 1435 0.0 17.36987 1 GTACTTT 25665 0.0 17.221188 14-15 GCGCCAT 2165 0.0 17.160967 2 AGTACTC 2085 0.0 17.129183 5 CTACACT 4755 0.0 16.307253 4 ACTTTTT 28160 0.0 15.8134575 16-17 ATTCGTC 815 0.0 15.569921 16-17 >>END_MODULE