##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765429_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17374404
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91966964737323	33.0	33.0	33.0	27.0	33.0
2	31.78757556230418	33.0	33.0	33.0	27.0	33.0
3	31.838954763570595	33.0	33.0	33.0	27.0	33.0
4	35.604259806552214	37.0	37.0	37.0	33.0	37.0
5	35.64272633467024	37.0	37.0	37.0	33.0	37.0
6	35.64727687925295	37.0	37.0	37.0	33.0	37.0
7	35.5709732546797	37.0	37.0	37.0	33.0	37.0
8	35.556668130889555	37.0	37.0	37.0	33.0	37.0
9	35.544306037778334	37.0	37.0	37.0	33.0	37.0
10-11	35.51192915739728	37.0	37.0	37.0	33.0	37.0
12-13	35.46780019044107	37.0	37.0	37.0	33.0	37.0
14-15	37.021590179438675	40.0	37.0	40.0	33.0	40.0
16-17	37.05818213965785	40.0	37.0	40.0	33.0	40.0
18-19	37.027216645819905	40.0	37.0	40.0	33.0	40.0
20-21	37.11926642203093	40.0	37.0	40.0	33.0	40.0
22-23	37.095799084676514	40.0	37.0	40.0	33.0	40.0
24-25	37.06964667680111	40.0	37.0	40.0	33.0	40.0
26-27	37.024099186366335	40.0	37.0	40.0	33.0	40.0
28-29	36.97189060413238	38.5	37.0	40.0	33.0	40.0
30-31	36.893830430097054	37.0	37.0	40.0	33.0	40.0
32-33	36.78477727926668	37.0	37.0	40.0	33.0	40.0
34-35	36.66655331026031	37.0	37.0	40.0	33.0	40.0
36-37	36.58113081173892	37.0	37.0	40.0	33.0	40.0
38-39	36.46953915656618	37.0	37.0	40.0	33.0	40.0
40-41	36.373245954220934	37.0	37.0	40.0	33.0	40.0
42-43	36.22524999994244	37.0	37.0	40.0	33.0	40.0
44-45	36.074619394138644	37.0	37.0	40.0	30.0	40.0
46-47	35.89705845449433	37.0	37.0	40.0	27.0	40.0
48-49	35.649408405606316	37.0	37.0	40.0	27.0	40.0
50-51	35.54114889351025	37.0	33.0	40.0	27.0	40.0
52-53	35.38296795677135	37.0	33.0	40.0	27.0	40.0
54-55	35.21654288112559	37.0	33.0	40.0	27.0	40.0
56-57	35.04567034356977	37.0	33.0	40.0	27.0	40.0
58-59	34.87817556216605	37.0	33.0	40.0	27.0	40.0
60-61	34.695071266905046	37.0	33.0	38.5	27.0	40.0
62-63	34.47608220115061	37.0	33.0	37.0	27.0	40.0
64-65	34.27712662834362	37.0	33.0	37.0	27.0	40.0
66-67	34.086523831263506	37.0	33.0	37.0	27.0	40.0
68-69	33.88813003312229	37.0	33.0	37.0	27.0	40.0
70-71	33.690403941338076	37.0	33.0	37.0	27.0	40.0
72-73	33.478415173262924	37.0	33.0	37.0	27.0	37.0
74-75	33.25449917591418	37.0	33.0	37.0	27.0	37.0
76-77	33.06488058525633	37.0	33.0	37.0	27.0	37.0
78-79	32.89897457777545	37.0	33.0	37.0	27.0	37.0
80-81	32.713319489980776	37.0	33.0	37.0	24.5	37.0
82-83	32.55840459333166	35.0	33.0	37.0	22.0	37.0
84-85	32.391925184886915	33.0	33.0	37.0	22.0	37.0
86-87	32.25437189097249	33.0	33.0	37.0	22.0	37.0
88-89	32.11414339737927	33.0	33.0	37.0	22.0	37.0
90-91	31.963278136044263	33.0	33.0	37.0	22.0	37.0
92-93	31.83993358851331	33.0	33.0	37.0	22.0	37.0
94-95	31.697925494307604	33.0	33.0	37.0	22.0	37.0
96-97	31.59493068078767	33.0	33.0	37.0	22.0	37.0
98-99	31.45991652433085	33.0	33.0	37.0	18.5	37.0
100	31.371979838848	33.0	33.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	14.0
7	239.0
8	1329.0
9	2601.0
10	3905.0
11	6446.0
12	12431.0
13	24122.0
14	36277.0
15	41713.0
16	46399.0
17	54113.0
18	64090.0
19	75955.0
20	89752.0
21	106417.0
22	125630.0
23	128772.0
24	130880.0
25	144693.0
26	169986.0
27	207214.0
28	258889.0
29	331907.0
30	427701.0
31	554205.0
32	723785.0
33	941263.0
34	1227455.0
35	1690039.0
36	2807808.0
37	4711207.0
38	2224484.0
39	2682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.754180995342786	19.51303474423565	12.681346092892534	25.051438167529028
2	16.29831180609873	21.21276761931546	37.629812531430915	24.85910804315489
3	17.93431584565167	26.462387542577414	29.505507658769154	26.09778895300176
4	12.694460920464138	17.244288576981287	37.661280087442336	32.399970415112236
5	13.91268261372307	37.63068762186892	33.645816052704205	14.8108137117038
6	30.82398107008448	38.15464403843723	17.522874453707878	13.49850043777041
7	27.01191361729588	32.05576433010307	22.86792110969677	18.064400942904285
8	26.36375901009324	32.95564555768359	20.580907408392253	20.099688023830918
9	26.896542753351426	14.571227882119006	20.265581484118822	38.26664788041075
10-11	25.415366765962162	25.448455670767185	27.345568227836765	21.790609335433896
12-13	26.067895623930465	23.614228724047166	27.02652131261596	23.291354339406407
14-15	23.956220311211826	24.460605958051858	24.695540635523383	26.887633095212937
16-17	23.60364315662617	26.683221167367705	25.500740935110095	24.21239474089603
18-19	23.90575370162478	26.004395567457948	27.290793617520247	22.799057113397026
20-21	25.487733062488594	25.31243374726791	25.891369851309264	23.308463338934235
22-23	24.539907545500714	24.988987550535963	26.06621606135176	24.404888842611562
24-25	23.91668717253566	25.71847816232878	26.329593350630216	24.03524131450535
26-27	24.1402545451207	25.595725208045973	26.092542652961065	24.17147759387226
28-29	23.746783734522715	25.602847656245615	26.62369936424669	24.02666924498498
30-31	24.317707138951373	25.40566374480332	26.30707476903151	23.969554347213805
32-33	23.86975675162426	25.569429542658657	25.826249696423787	24.7345640092933
34-35	24.562339679203994	25.39979336294822	26.41275067521709	23.625116282630696
36-37	24.37044911339362	25.343717805642367	26.023252784490836	24.262580296473175
38-39	24.309315565757174	24.95738505853661	26.318737425641892	24.414561950064325
40-41	24.186895200094465	25.27710606974892	26.573358471503745	23.96264025865287
42-43	24.762539362817783	25.117939860105036	26.223476465924513	23.896044311152668
44-45	24.028459728703293	25.558567494483107	25.897365290760426	24.515607486053174
46-47	23.75686759356459	25.261774992281033	26.355699675586013	24.625657738568364
48-49	24.499466096401978	25.49543663528484	25.838018541277513	24.16707872703567
50-51	23.70754011549842	25.717309109159338	25.702581910723477	24.872568864618767
52-53	23.735534635985157	25.55864287904484	25.699460793384116	25.006361691585884
54-55	23.975526122335882	25.420044954863503	25.836757990144655	24.76767093265596
56-57	23.907918675042964	25.032985485005067	25.5887004602512	25.470395379700765
58-59	23.5787335278183	25.034541445633216	26.10115492179802	25.28557010475046
60-61	23.651089723260796	25.19568298053282	25.84563295271976	25.307594343486628
62-63	23.31665096641124	25.173670673088616	25.697212089999756	25.81246627050039
64-65	23.212733686962437	24.80490505679715	25.899463512290932	26.082897743949484
66-67	23.642399436944693	24.986572231579263	25.702016627608298	25.669011703867746
68-69	23.74715453226403	24.63684715771248	25.68493766700642	25.93106064301707
70-71	23.056150331087956	24.514254336829573	26.022629454589087	26.406965877493384
72-73	23.6422580028689	24.552393665397997	25.996418481601978	25.80892985013113
74-75	23.60118358892603	24.52464472517865	25.985772747000745	25.888398938894575
76-77	23.272166121795294	24.725444892107525	25.997371044669993	26.005017941427187
78-79	23.38581399076806	24.747874406743417	25.899014498326032	25.96729710416249
80-81	23.07938586854154	24.488033605450223	26.263667179071216	26.168913346937018
82-83	23.3189775097405	24.550228192499226	25.96865980606141	26.162134491698865
84-85	23.358202185779138	24.58306813732888	25.596885266452485	26.461844410439493
86-87	22.93513710219456	24.379989228152365	25.896143330325483	26.78873033932759
88-89	23.162704073684854	24.042070751021548	25.9563478819769	26.8388772933167
90-91	23.404252135177327	24.062124777173956	25.857317832739863	26.67630525490885
92-93	23.2636224399159	24.221358099774264	25.655501045961255	26.859518414348578
94-95	22.57398564523048	24.227896859175416	26.22471368940853	26.973403806185576
96-97	23.14747364226987	24.05883503228715	25.81458196066604	26.979109364776942
98-99	22.918545480310023	24.069750004122515	25.859703922040538	27.152000593526925
100	23.001076434443	24.1117056000722	25.619369972164197	27.267847993320604
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1138.0
1	1013.5
2	1203.0
3	1550.5
4	1254.0
5	1030.0
6	1378.5
7	2683.0
8	4107.0
9	3802.5
10	2435.0
11	1754.0
12	1854.0
13	2121.5
14	2485.0
15	3031.5
16	3705.0
17	4463.5
18	5444.5
19	6527.0
20	7981.5
21	10024.0
22	12728.5
23	16770.0
24	21558.5
25	27786.5
26	39513.5
27	53929.5
28	67096.5
29	88714.5
30	112729.5
31	133484.5
32	164804.5
33	199676.5
34	237418.5
35	267090.5
36	294283.5
37	332841.0
38	347252.0
39	349732.0
40	362454.5
41	372811.5
42	385491.0
43	429703.5
44	489288.5
45	538557.0
46	607946.0
47	677036.0
48	737047.5
49	782442.5
50	806624.5
51	794481.0
52	769028.5
53	768556.0
54	737424.5
55	692041.0
56	661100.0
57	606777.5
58	566557.5
59	524811.0
60	447848.0
61	361774.0
62	290350.5
63	227313.5
64	168774.0
65	135817.0
66	109505.5
67	80849.5
68	62011.5
69	52071.5
70	46679.0
71	42578.5
72	38405.0
73	41240.5
74	32425.0
75	22578.5
76	17525.5
77	12700.5
78	9436.5
79	5920.0
80	4214.0
81	3416.5
82	3132.0
83	3022.0
84	2354.0
85	1586.0
86	1147.0
87	613.5
88	273.0
89	208.0
90	165.0
91	136.0
92	95.0
93	64.0
94	43.0
95	35.5
96	31.5
97	14.5
98	9.0
99	10.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017623626111146026
2	0.0056692592160283595
3	0.0060491283614678235
4	0.00385049179240911
5	0.045451918811143105
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	4.345472800102956E-4
18-19	0.0017871116614992953
20-21	6.935489700826572E-4
22-23	0.005093699904756445
24-25	0.007649183246803746
26-27	0.00587358277152989
28-29	0.022495735681062785
30-31	0.020619412326316346
32-33	0.013381753987072018
34-35	0.013804790080856874
36-37	0.013681044828933413
38-39	0.021502895869118733
40-41	0.030694578070131213
42-43	0.018167529660297988
44-45	0.017154545272459416
46-47	0.0186222215162028
48-49	0.013548666187340871
50-51	0.010276611502760036
52-53	0.0276844028721791
54-55	0.026113125952406772
56-57	0.01363500008403166
58-59	0.01658474155430022
60-61	0.017652404076709625
62-63	0.02110863774089747
64-65	0.011309740466493124
66-67	0.015848025635872173
68-69	0.007951351885221501
70-71	0.009229093556245153
72-73	0.010843537424362873
74-75	0.0036576794231330183
76-77	0.007732639346938174
78-79	0.005864949381860811
80-81	0.0025123163937019076
82-83	2.187125382833276E-4
84-85	0.002659084018076246
86-87	0.002368426565883929
88-89	0.0011252184535365934
90-91	8.316832047879168E-4
92-93	0.0011194628604238742
94-95	0.0030331975704029905
96-97	0.0013324198055944824
98-99	0.0017122889510339462
100	0.0021756141966078374
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.7374404E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.51612229897618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41902192408341	23.8730189595471
2	14.449903786967432	9.397096774909441
3	4.93212608403371	4.811208448272318
4	2.2555014376619673	2.9336064237013253
5	1.1677890509976439	1.898598580082236
6	0.7296543373993605	1.4235317802513623
7	0.4898910860841184	1.1150550927802623
8	0.35004026254795123	0.9105561589259931
9	0.2612773120117119	0.7646152528188525
>10	1.545044246854236	9.679998850584488
>50	0.17758691810035215	4.043951625602517
>100	0.1690653637452394	11.570503669108117
>500	0.02671411453786252	6.07147457789114
>1k	0.023786626385433763	14.763360933291716
>5k	0.002081517020474491	4.582887574408649
>10k+	5.15931569177438E-4	2.1605352978243655
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	25384	0.14609997557326282	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	24732	0.14234732886376994	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21164	0.12181137263758803	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.755593112719147E-6
2	0.0	0.0	0.0	0.0	5.755593112719147E-6
3	0.0	0.0	0.0	0.0	5.755593112719147E-6
4	0.0	5.755593112719147E-6	0.0	5.755593112719147E-6	5.755593112719147E-6
5	5.755593112719147E-6	5.755593112719147E-6	0.0	5.755593112719147E-6	5.755593112719147E-6
6	1.1511186225438294E-5	5.755593112719147E-6	0.0	5.755593112719147E-6	1.726677933815744E-5
7	1.726677933815744E-5	5.755593112719147E-6	0.0	1.726677933815744E-5	1.726677933815744E-5
8	1.726677933815744E-5	1.1511186225438294E-5	0.0	1.726677933815744E-5	1.726677933815744E-5
9	1.726677933815744E-5	1.1511186225438294E-5	0.0	4.028915178903403E-5	1.726677933815744E-5
10-11	1.726677933815744E-5	1.1511186225438294E-5	0.0	4.6044744901753177E-5	3.741135523267446E-5
12-13	2.877796556359574E-5	1.1511186225438294E-5	0.0	5.180033801447233E-5	6.906711735262976E-5
14-15	4.6044744901753177E-5	1.1511186225438294E-5	0.0	5.46781345708319E-5	9.78450829162255E-5
16-17	5.180033801447233E-5	1.1511186225438294E-5	0.0	6.331152423991061E-5	1.2662304847982123E-4
18-19	5.755593112719147E-5	1.1511186225438294E-5	0.0	9.208948980350635E-5	1.4964542093069783E-4
20-21	7.770050702170849E-5	1.1511186225438294E-5	0.0	1.841789796070127E-4	1.726677933815744E-4
22-23	8.921169324714679E-5	1.1511186225438294E-5	0.0	6.417486320681848E-4	1.841789796070127E-4
24-25	1.1511186225438294E-4	1.4388982781797868E-5	0.0	0.002043235555015297	1.8993457271973186E-4
26-27	1.525232174870574E-4	1.726677933815744E-5	0.0	0.0038763919614163457	1.9281236927609142E-4
28-29	1.6691220026885525E-4	1.726677933815744E-5	0.0	0.015836514449646732	1.95690165832451E-4
30-31	1.726677933815744E-4	1.726677933815744E-5	0.0	0.06494611268392286	2.0144575894517016E-4
32-33	1.726677933815744E-4	1.726677933815744E-5	0.0	0.1657783484256496	2.1295694517060843E-4
34-35	1.726677933815744E-4	2.3022372450876588E-5	0.0	0.2987670828881382	2.3597931762148503E-4
36-37	1.726677933815744E-4	2.8777965563595735E-5	0.0	0.5147514700360369	2.3597931762148503E-4
38-39	1.726677933815744E-4	3.165576211995531E-5	0.0	0.8761451615836722	2.503683004032829E-4
40-41	1.726677933815744E-4	3.453355867631488E-5	0.0	1.3139932742441123	2.5324609695964246E-4
42-43	1.8130118305065312E-4	3.741135523267446E-5	0.0	1.6982194036699043	2.6475728318508076E-4
44-45	1.8993457271973186E-4	4.028915178903403E-5	0.0	2.13388902433718	2.9065745219231695E-4
46-47	1.8993457271973186E-4	4.028915178903403E-5	0.0	2.625551932601544	3.1080202808683396E-4
48-49	1.8993457271973186E-4	4.028915178903403E-5	0.0	3.1441797945989975	3.1367982464319355E-4
50-51	1.95690165832451E-4	4.028915178903403E-5	0.0	3.68917978423893	3.280688074249914E-4
52-53	1.95690165832451E-4	4.028915178903403E-5	0.0	4.215451073890074	3.6260236610130623E-4
54-55	1.95690165832451E-4	4.028915178903403E-5	0.0	4.7313536625486545	3.91380331664902E-4
56-57	1.95690165832451E-4	4.028915178903403E-5	0.0	5.316087389242243	3.971359247776212E-4
58-59	1.95690165832451E-4	4.028915178903403E-5	0.0	5.960270637197109	4.028915178903403E-4
60-61	1.985679623888106E-4	4.028915178903403E-5	0.0	6.6262790942354055	4.028915178903403E-4
62-63	2.0144575894517016E-4	4.028915178903403E-5	0.0	7.282615277047777	4.3166948345393604E-4
64-65	2.072013520578893E-4	4.6044744901753177E-5	0.0	7.922432907626644	4.4030287312301475E-4
66-67	2.1007914861424887E-4	4.6044744901753177E-5	0.0	8.612309809303387	4.4318066967937434E-4
68-69	2.1583474172696802E-4	4.6044744901753177E-5	0.0	9.371259583925871	4.4318066967937434E-4
70-71	2.1871253828332758E-4	4.6044744901753177E-5	0.0	10.218097265379578	4.4318066967937434E-4
72-73	2.2159033483968714E-4	4.6044744901753177E-5	0.0	11.025330710624663	4.575696524611722E-4
74-75	2.2446813139604673E-4	5.180033801447233E-5	0.0	11.789909455311388	4.7483643179932966E-4
76-77	2.2446813139604673E-4	6.043372768355104E-5	0.0	12.602688414520578	4.777142283556892E-4
78-79	2.2446813139604673E-4	6.331152423991061E-5	0.0	13.443350344564337	4.8346982146840837E-4
80-81	2.2446813139604673E-4	6.618932079627019E-5	0.0	14.35644353613511	4.921032111374871E-4
82-83	2.2446813139604673E-4	7.482271046534892E-5	0.0	15.29718371922283	5.237589732574423E-4
84-85	2.2446813139604673E-4	8.345610013442764E-5	0.0	16.18288892096673	5.295145663701615E-4
86-87	2.2446813139604673E-4	1.0360067602894465E-4	0.0	17.06713795765311	5.439035491519595E-4
88	2.2446813139604673E-4	1.0935626914166379E-4	0.0	17.741161078100866	5.582925319337573E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	65485	0.0	25.234869	1
GTACTAG	6995	0.0	21.226082	1
CAACGCA	73500	0.0	21.19034	5
ATCAACG	74425	0.0	20.902925	3
AACGCAG	76505	0.0	20.638906	6
GTACATG	109665	0.0	19.721659	1
TACATGG	106785	0.0	19.668285	2
TCAACGC	79875	0.0	19.56402	4
CATGGGG	43660	0.0	19.52092	4
TATCAAC	85530	0.0	19.414103	2
AATACGG	3260	0.0	19.312075	5
TATTCGC	1050	0.0	19.239206	9
ACGCAGA	80745	0.0	19.083858	7
CGCAGAG	81080	0.0	18.860153	8
CTATTGA	8180	0.0	18.780294	9
ACATGGG	104730	0.0	18.636406	3
GTACAAG	6985	0.0	18.633045	1
GTGATCG	4225	0.0	18.4582	8
TAGGCAT	9280	0.0	17.973064	5
GCAGAGT	83115	0.0	17.929234	9
>>END_MODULE