FastQCFastQC Report
Fri 27 May 2016
ERR765428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765428_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16662104
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA739300.44370146771380137No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT700660.4205111191239713No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA324790.19492736331498112No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG291210.1747738460881051No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC263980.15843137217244593No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262370.1574651076478697No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC252800.1517215352875003No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA250000.15004107524475901No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC247390.14847464641920372No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG244010.14644609108189457No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA222400.1334765405377376No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG217860.13075179461129277No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC214150.12852518505466057No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC206770.12409597251343528No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC202020.12124519208378484No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC199820.11992483062163099No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT170840.10253206917925851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTGA131000.025.4002279
GTACTAG85700.024.6785161
AGGGCTA142500.023.6801915
ATTTAGG153400.022.9479451
TTTAGGG148650.022.8587722
GGGCTAT153050.022.6620226
TAGTACT102600.022.2620184
GGCTATT157000.021.9721227
TATTCGC13250.021.636639
TAGGCAT112750.021.3833855
GCTATTG166200.020.5579158
GTACAAG71100.020.1612571
TACGGGC58150.020.1245084
CATGGGG491700.020.0913054
CTGTGCG109100.020.0741339
ACGTTAA17350.020.0450828
GTGATCG65000.019.738998
GGCGTCG60150.019.6897668
ATACGGG60200.019.6732943
CGGGCGT60850.019.3087886
Produced by FastQC (version 0.11.5)