##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765428_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16662104
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.264437072292914	33.0	33.0	33.0	33.0	33.0
2	32.19627095113558	33.0	33.0	33.0	33.0	33.0
3	32.2078964337277	33.0	33.0	33.0	33.0	33.0
4	36.020427972361716	37.0	37.0	37.0	33.0	37.0
5	36.01762880606195	37.0	37.0	37.0	33.0	37.0
6	36.02477772314949	37.0	37.0	37.0	33.0	37.0
7	35.95930207853702	37.0	37.0	37.0	33.0	37.0
8	35.94399488804055	37.0	37.0	37.0	33.0	37.0
9	35.92670049352711	37.0	37.0	37.0	33.0	37.0
10-11	35.920250857874855	37.0	37.0	37.0	33.0	37.0
12-13	35.90839581243762	37.0	37.0	37.0	33.0	37.0
14-15	37.84667758645607	40.0	37.0	40.0	33.0	40.0
16-17	37.891875119732774	40.0	37.0	40.0	33.0	40.0
18-19	37.9410172928941	40.0	37.0	40.0	33.0	40.0
20-21	37.970704420042026	40.0	37.0	40.0	33.0	40.0
22-23	37.973465355875824	40.0	37.0	40.0	33.0	40.0
24-25	37.989973805228914	40.0	37.0	40.0	33.0	40.0
26-27	37.97873119745262	40.0	37.0	40.0	33.0	40.0
28-29	37.953885115589244	40.0	37.0	40.0	33.0	40.0
30-31	37.897397831630386	40.0	37.0	40.0	33.0	40.0
32-33	37.82278042436897	40.0	37.0	40.0	33.0	40.0
34-35	37.73281921658873	40.0	37.0	40.0	33.0	40.0
36-37	37.65706962938174	40.0	37.0	40.0	33.0	40.0
38-39	37.53624512246472	40.0	37.0	40.0	33.0	40.0
40-41	37.44685149006392	40.0	37.0	40.0	33.0	40.0
42-43	37.309665424006475	40.0	37.0	40.0	33.0	40.0
44-45	37.1186918530817	40.0	37.0	40.0	33.0	40.0
46-47	36.92559610118866	40.0	37.0	40.0	33.0	40.0
48-49	36.73216332703241	38.5	37.0	40.0	33.0	40.0
50-51	36.556093786234925	37.0	37.0	40.0	33.0	40.0
52-53	36.3936384924737	37.0	37.0	40.0	33.0	40.0
54-55	36.266463797129106	37.0	37.0	40.0	33.0	40.0
56-57	36.10118761112042	37.0	37.0	40.0	33.0	40.0
58-59	35.91286877695638	37.0	37.0	40.0	33.0	40.0
60-61	35.72335570585804	37.0	37.0	40.0	33.0	40.0
62-63	35.50903028213004	37.0	37.0	40.0	27.0	40.0
64-65	35.3049922446769	37.0	33.0	38.5	27.0	40.0
66-67	35.102225325205026	37.0	33.0	37.0	27.0	40.0
68-69	34.89529221519683	37.0	33.0	37.0	27.0	40.0
70-71	34.68182979772543	37.0	33.0	37.0	27.0	40.0
72-73	34.46794564479972	37.0	33.0	37.0	27.0	40.0
74-75	34.24344626584974	37.0	33.0	37.0	27.0	37.0
76-77	34.0382044188417	37.0	33.0	37.0	27.0	37.0
78-79	33.8570998896658	37.0	33.0	37.0	27.0	37.0
80-81	33.71497792235603	37.0	33.0	37.0	27.0	37.0
82-83	33.571190379078175	37.0	33.0	37.0	27.0	37.0
84-85	33.4418769082224	37.0	33.0	37.0	27.0	37.0
86-87	33.33398813259117	37.0	33.0	37.0	27.0	37.0
88-89	33.241158799632984	37.0	33.0	37.0	27.0	37.0
90-91	33.116096172488184	37.0	33.0	37.0	27.0	37.0
92-93	33.00357028740188	37.0	33.0	37.0	27.0	37.0
94-95	32.93228433215877	37.0	33.0	37.0	27.0	37.0
96-97	32.874937642929126	37.0	33.0	37.0	27.0	37.0
98-99	32.76808703150574	37.0	33.0	37.0	27.0	37.0
100	32.669947144730344	37.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	188.0
8	1752.0
9	3975.0
10	4292.0
11	5258.0
12	7694.0
13	13006.0
14	23204.0
15	31124.0
16	34804.0
17	40976.0
18	49702.0
19	59587.0
20	70634.0
21	87092.0
22	108635.0
23	112754.0
24	103048.0
25	99853.0
26	103755.0
27	116807.0
28	137475.0
29	173976.0
30	227285.0
31	298683.0
32	410962.0
33	580397.0
34	828100.0
35	1256532.0
36	2424849.0
37	5550470.0
38	3688630.0
39	6603.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01769378406523	19.7069037730918	12.567209956470862	24.708192486372106
2	15.45756594208248	20.860258420822987	39.086278513285045	24.59589712380949
3	17.65080808522141	27.413362682167868	29.8341193885238	25.101709844086916
4	12.225952976886953	17.803219809455037	38.17239407460186	31.798433139056147
5	12.958036177293419	38.51200529918888	34.43936485401858	14.090593669499127
6	30.16204316093574	38.2095022333314	18.146267722251643	13.482186883481223
7	26.240468185995326	32.59544897592716	23.31033262561925	17.853750212458262
8	26.56654885841548	33.815693384220864	20.254620905018957	19.3631368523447
9	27.115987272675767	14.615681188882268	20.134540031679073	38.13379150676289
10-11	24.779880241936002	26.179690390885924	27.66167550198552	21.37875386519255
12-13	25.904558631971085	24.24487927815119	27.34795377582567	22.502608314052054
14-15	23.83570526267271	24.520321683264008	25.30603878117674	26.337934272886542
16-17	22.773456461440883	27.032942538349296	26.449678864085836	23.743922136123985
18-19	23.04248911181925	26.78220289586477	28.28970458952843	21.88560340278755
20-21	24.285738463761838	25.871153486978592	27.13350306779984	22.709604981459723
22-23	23.88123068817996	25.674302054202837	26.609395733569258	23.835071524047944
24-25	23.269070990683616	26.456486747131557	26.97174662156292	23.302695640621902
26-27	23.565600476603006	26.069287665100504	26.75593040258976	23.60918145570673
28-29	23.029868004314018	26.143049678355627	27.312725874511294	23.51435644281906
30-31	23.87984434618821	25.858967150847217	26.971260652316175	23.289927850648393
32-33	23.195439126932165	26.462643633838358	26.575423718285236	23.766493520944245
34-35	23.39921839850465	26.296997345138028	27.286895269894863	23.016888986462458
36-37	23.707975231323722	26.036242084465556	27.00400162800671	23.251781056204013
38-39	23.86037265874451	25.565852955757833	27.159237407357384	23.414536978140273
40-41	23.353180873345575	25.681771116645365	27.43911278642363	23.52593522358543
42-43	24.029417293278208	25.62229535957764	26.836250691989438	23.512036655154713
44-45	23.695218803099536	26.14942866759204	26.745613879255586	23.409738650052837
46-47	23.335978457462513	25.86407454904855	27.13900657443982	23.66094041904912
48-49	24.008622556139446	25.861246574178708	26.765634287198253	23.364496582483593
50-51	23.27410215699701	26.288011536169016	26.221621068332578	24.216265238501393
52-53	22.90271750193423	26.51833379814085	25.87517983969587	24.70376886022904
54-55	23.21225228255244	26.536931546488113	25.688296198616218	24.562519972343228
56-57	23.757697382535085	25.737789331451737	25.894690603205788	24.609822682807387
58-59	23.277148849375966	25.673809739658182	26.76886206786761	24.28017934309824
60-61	23.376718585328497	26.255368516068938	26.075938845129865	24.2919740534727
62-63	22.98572855787662	26.013269509919873	26.085230496622035	24.915771435581473
64-65	22.60590886593967	26.047670366703645	26.313679075635044	25.032741691721643
66-67	23.558648994278983	25.744045940013716	26.05100348840085	24.646301577306453
68-69	23.434576449648855	25.554323751670253	26.07681178799508	24.93428801068581
70-71	23.104205891208906	25.685077992358817	26.23957955791957	24.97113655851271
72-73	23.91126052106692	25.534170250347998	26.2952169598745	24.259352268710575
74-75	23.788870187559535	25.72236310405842	26.249515119537627	24.239251588844414
76-77	23.24077948997428	25.88302428617451	26.496329982148453	24.379866241702754
78-79	23.27476879179948	26.050953731951537	26.30649663683522	24.367780839413758
80-81	22.915951111886972	26.050278363751183	26.511690593317994	24.52207993104385
82-83	23.214690185796936	25.732801206869254	26.39199760124173	24.660511006092083
84-85	23.575583966168224	25.871896927399174	25.724349237052586	24.828169869380023
86-87	23.02856587789586	25.7129211089058	26.227764272548583	25.030748740649756
88-89	23.42805449797993	25.038662404154238	26.352974080719456	25.180309017146378
90-91	23.440617767439722	25.17702639823172	26.42909824551738	24.95325758881118
92-93	23.19586955979617	25.45573223596546	26.245441942415315	25.102956261823056
94-95	22.288646786131054	25.602956311881854	26.74863399702848	25.359762904958615
96-97	23.115985656362284	25.349403132146957	26.196677275923573	25.33793393556718
98-99	22.96676338114322	25.593985609500457	25.96567636356129	25.473574645795033
100	22.916439102347887	25.57704509602594	25.959335146485802	25.547180655140366
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1398.0
1	1201.5
2	1474.0
3	1916.5
4	1554.5
5	1330.0
6	1755.5
7	3464.5
8	5587.0
9	5315.5
10	3389.5
11	2495.0
12	2717.5
13	3197.5
14	3868.0
15	4992.5
16	6122.0
17	7304.0
18	8829.5
19	10572.5
20	12759.0
21	15171.5
22	18311.0
23	22227.5
24	26693.5
25	32828.0
26	43059.0
27	55165.5
28	68877.5
29	95543.5
30	129250.5
31	158817.5
32	200186.5
33	239139.0
34	276114.5
35	309299.0
36	336111.5
37	389011.5
38	412478.5
39	390969.0
40	377626.0
41	376363.5
42	393647.5
43	431070.0
44	469155.5
45	521874.0
46	593503.5
47	663161.0
48	748813.5
49	804419.5
50	871248.5
51	867544.0
52	760453.0
53	714007.0
54	675515.5
55	610902.0
56	558905.0
57	495140.0
58	442751.5
59	407465.5
60	338306.5
61	263732.5
62	210469.5
63	166189.0
64	123718.5
65	90711.0
66	69552.5
67	53334.0
68	42461.0
69	35278.0
70	30824.0
71	27075.0
72	23136.5
73	23106.5
74	18665.5
75	13715.0
76	10595.5
77	7578.5
78	5719.0
79	3903.0
80	2917.0
81	2203.5
82	1599.5
83	1230.5
84	898.5
85	638.0
86	460.5
87	253.5
88	124.0
89	90.5
90	74.0
91	57.0
92	38.5
93	23.5
94	18.0
95	14.5
96	8.5
97	6.0
98	4.0
99	2.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0823125338792748
2	8.042201633119083E-4
3	0.0
4	0.0
5	0.06301124995978899
6	0.0
7	4.8013144078322877E-5
8	0.0
9	0.0
10-11	5.671552644251891E-4
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0011433129933650637
24-25	0.0013233622836587744
26-27	1.1103039568112167E-4
28-29	0.003676006343496595
30-31	0.0
32-33	1.8605093330350116E-4
34-35	1.950533978181867E-4
36-37	6.301725160279879E-5
38-39	1.4103861073007346E-4
40-41	3.300903655384698E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0013413672126881455
50-51	4.4112076121959146E-4
52-53	0.006514783487127436
54-55	3.0008215048951798E-6
56-57	0.002115579160951102
58-59	0.0017854887954126322
60-61	8.642365934098118E-4
62-63	0.0017884896169175274
64-65	0.0014614000728839528
66-67	1.680460042741301E-4
68-69	0.0
70-71	1.380377892251783E-4
72-73	7.051930536503673E-4
74-75	0.004687283190646271
76-77	0.0064907769150882744
78-79	0.0042851731089903165
80-81	0.006484775272078485
82-83	9.002464514685541E-4
84-85	0.0022356120211469094
86-87	2.3706489888671923E-4
88-89	0.0034149348725707154
90-91	2.4606736340140477E-4
92-93	0.003231884760772109
94-95	0.0015754312900699697
96-97	4.951355483077047E-4
98-99	0.0
100	8.882431654489734E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.6662104E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.455348107342235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.94932427158145	16.37269030612861
2	15.172757383157276	7.4211012710671636
3	6.239737863704878	4.577848846664003
4	3.2567117442569216	3.1857607756429096
5	1.8888272577143643	2.30959640510207
6	1.2176351970477506	1.7866615566933
7	0.8533742548968162	1.460869512854181
8	0.6072038144907379	1.1879504524381643
9	0.47574011245198267	1.047095105277544
>10	2.761519446551026	12.62373377701332
>50	0.2618176791328049	4.474749368097217
>100	0.23878308005042276	12.14973654486812
>500	0.04058922977956037	6.899770476665287
>1k	0.03262328132801344	15.828025920689562
>5k	0.0021797749139235555	3.384237634352653
>10k+	0.0011756089423407938	5.290172046445852
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	73930	0.44370146771380137	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	70066	0.4205111191239713	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	32479	0.19492736331498112	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	29121	0.1747738460881051	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	26398	0.15843137217244593	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26237	0.1574651076478697	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	25280	0.1517215352875003	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	25000	0.15004107524475901	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	24739	0.14847464641920372	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	24401	0.14644609108189457	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	22240	0.1334765405377376	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	21786	0.13075179461129277	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	21415	0.12852518505466057	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	20677	0.12409597251343528	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	20202	0.12124519208378484	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	19982	0.11992483062163099	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	17084	0.10253206917925851	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	6.00164300979036E-6	0.0	0.0	0.0
6	0.0	6.00164300979036E-6	0.0	0.0	6.00164300979036E-6
7	0.0	6.00164300979036E-6	0.0	0.0	6.00164300979036E-6
8	0.0	6.00164300979036E-6	0.0	0.0	6.00164300979036E-6
9	0.0	6.00164300979036E-6	0.0	0.0	6.00164300979036E-6
10-11	6.00164300979036E-6	6.00164300979036E-6	0.0	2.400657203916144E-5	6.00164300979036E-6
12-13	1.200328601958072E-5	6.00164300979036E-6	0.0	6.601807310769396E-5	6.00164300979036E-6
14-15	2.400657203916144E-5	6.00164300979036E-6	0.0	9.602628815664577E-5	6.00164300979036E-6
16-17	3.300903655384698E-5	6.00164300979036E-6	0.0	1.4403943223496862E-4	6.00164300979036E-6
18-19	3.600985805874216E-5	6.00164300979036E-6	0.0	2.0705668383776742E-4	6.00164300979036E-6
20-21	4.201150106853252E-5	6.00164300979036E-6	0.0	3.33091187043365E-4	9.00246451468554E-6
22-23	4.801314407832288E-5	6.00164300979036E-6	0.0	8.372291998657551E-4	1.200328601958072E-5
24-25	9.602628815664575E-5	6.00164300979036E-6	0.0	0.0018935183695888585	1.200328601958072E-5
26-27	1.5904353975944454E-4	6.00164300979036E-6	0.0	0.0033609200854826018	1.200328601958072E-5
28-29	1.6804600427413008E-4	6.00164300979036E-6	0.0	0.013545708273096842	1.200328601958072E-5
30-31	1.7404764728392046E-4	6.00164300979036E-6	0.0	0.0504918226413663	1.200328601958072E-5
32-33	1.800492902937108E-4	6.00164300979036E-6	0.0	0.11849944040680577	1.200328601958072E-5
34-35	1.800492902937108E-4	9.00246451468554E-6	0.0	0.2061294299927548	1.200328601958072E-5
36-37	1.83050111798606E-4	1.200328601958072E-5	0.0	0.34468936215978485	1.200328601958072E-5
38-39	1.8605093330350116E-4	1.200328601958072E-5	0.0	0.5807699915928985	1.200328601958072E-5
40-41	1.8605093330350116E-4	1.200328601958072E-5	0.0	0.8776052532141199	1.200328601958072E-5
42-43	1.9205257631329153E-4	1.200328601958072E-5	0.0	1.1265654085462435	2.100575053426626E-5
44-45	1.9805421932308188E-4	1.200328601958072E-5	0.0	1.3957270942493216	2.400657203916144E-5
46-47	2.0405586233287223E-4	1.5004107524475901E-5	0.0	1.6920372121071865	2.400657203916144E-5
48-49	2.0405586233287223E-4	1.800492902937108E-5	0.0	2.0097551905809734	2.400657203916144E-5
50-51	2.100575053426626E-4	1.800492902937108E-5	0.0	2.3560920037469457	2.400657203916144E-5
52-53	2.2206079136224334E-4	1.800492902937108E-5	0.0	2.690914664798635	2.400657203916144E-5
54-55	2.2206079136224334E-4	2.7007393544056624E-5	0.0	3.0001733274501228	2.400657203916144E-5
56-57	2.2506161286713853E-4	3.0008215048951802E-5	0.0	3.3542282535266854	2.400657203916144E-5
58-59	2.3406407738182404E-4	3.0008215048951802E-5	0.0	3.7581568330146062	2.400657203916144E-5
60-61	2.3706489888671923E-4	3.0008215048951802E-5	0.0	4.175922200461598	2.400657203916144E-5
62-63	2.430665418965096E-4	3.0008215048951802E-5	0.0	4.593327469328003	2.7007393544056624E-5
64-65	2.4606736340140477E-4	3.0008215048951802E-5	0.0	5.003788837232081	3.600985805874216E-5
66-67	2.4606736340140477E-4	3.0008215048951802E-5	0.0	5.435565040285429	3.600985805874216E-5
68-69	2.4606736340140477E-4	3.0008215048951802E-5	0.0	5.934910741164501	4.201150106853252E-5
70-71	2.490681849063E-4	3.300903655384698E-5	0.0	6.513010001618043	4.201150106853252E-5
72-73	2.5206900641119514E-4	3.600985805874216E-5	0.0	7.06233738548265	4.201150106853252E-5
74-75	2.5807064942098547E-4	3.600985805874216E-5	0.0	7.583589083347457	4.50123225734277E-5
76-77	2.5807064942098547E-4	3.600985805874216E-5	0.0	8.133357587973283	4.801314407832288E-5
78-79	2.5807064942098547E-4	3.600985805874216E-5	0.0	8.708317989132706	4.801314407832288E-5
80-81	2.5807064942098547E-4	3.600985805874216E-5	0.0	9.3505718125394	4.801314407832288E-5
82-83	2.6407229243077584E-4	3.600985805874216E-5	0.0	10.044787861124862	5.401478708811324E-5
84-85	2.7907639995525176E-4	3.600985805874216E-5	0.0	10.696149177798915	5.401478708811324E-5
86-87	2.940805074797276E-4	3.9010679563637344E-5	0.0	11.349157945479154	5.401478708811324E-5
88	2.940805074797276E-4	4.201150106853252E-5	0.0	11.855747629471043	5.401478708811324E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATTGA	13100	0.0	25.400227	9
GTACTAG	8570	0.0	24.678516	1
AGGGCTA	14250	0.0	23.680191	5
ATTTAGG	15340	0.0	22.947945	1
TTTAGGG	14865	0.0	22.858772	2
GGGCTAT	15305	0.0	22.662022	6
TAGTACT	10260	0.0	22.262018	4
GGCTATT	15700	0.0	21.972122	7
TATTCGC	1325	0.0	21.63663	9
TAGGCAT	11275	0.0	21.383385	5
GCTATTG	16620	0.0	20.557915	8
GTACAAG	7110	0.0	20.161257	1
TACGGGC	5815	0.0	20.124508	4
CATGGGG	49170	0.0	20.091305	4
CTGTGCG	10910	0.0	20.074133	9
ACGTTAA	1735	0.0	20.045082	8
GTGATCG	6500	0.0	19.73899	8
GGCGTCG	6015	0.0	19.689766	8
ATACGGG	6020	0.0	19.673294	3
CGGGCGT	6085	0.0	19.308788	6
>>END_MODULE