##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765425_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8143545 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21647759053336 33.0 31.0 34.0 31.0 34.0 2 32.20572907744723 34.0 31.0 34.0 30.0 34.0 3 32.22487516186133 34.0 31.0 34.0 30.0 34.0 4 35.6983684623834 37.0 35.0 37.0 35.0 37.0 5 35.72885236098038 37.0 35.0 37.0 35.0 37.0 6 35.84824581923474 37.0 37.0 37.0 35.0 37.0 7 35.807448476062945 37.0 36.0 37.0 35.0 37.0 8 35.85320778604404 37.0 37.0 37.0 35.0 37.0 9 37.53420040043986 39.0 38.0 39.0 35.0 39.0 10-11 37.46943382765123 39.0 38.0 39.0 35.0 39.0 12-13 37.45287384056944 39.0 38.0 39.0 35.0 39.0 14-15 38.802179456244176 41.0 38.5 41.0 35.0 41.0 16-17 38.754615465377796 40.0 38.0 41.0 35.0 41.0 18-19 38.75032482782375 40.0 38.5 41.0 35.0 41.0 20-21 38.737886325918254 40.5 39.0 41.0 35.0 41.0 22-23 38.65424468090985 40.0 39.0 41.0 34.5 41.0 24-25 38.581314587197596 40.5 38.0 41.0 34.0 41.0 26-27 38.44274176663849 40.0 38.0 41.0 34.0 41.0 28-29 38.301408968698524 40.0 38.0 41.0 34.0 41.0 30-31 38.06492068257742 40.0 38.0 41.0 34.0 41.0 32-33 37.752634509909385 40.0 37.0 41.0 33.0 41.0 34-35 37.61407071490365 40.0 37.0 41.0 33.0 41.0 36-37 37.478998826678065 40.0 37.0 41.0 33.0 41.0 38-39 37.28553080998509 40.0 37.0 41.0 32.0 41.0 40-41 37.01343917176119 40.0 36.0 41.0 31.0 41.0 42-43 36.73654360600942 40.0 36.0 41.0 31.0 41.0 44-45 36.35255567446364 40.0 35.5 41.0 30.0 41.0 46-47 35.884772847697164 39.0 35.0 41.0 28.5 41.0 48-49 35.45553846635587 39.0 35.0 41.0 27.0 41.0 50-51 34.21080567492413 38.0 34.0 40.0 24.5 40.5 52-53 34.156045677895804 38.0 34.0 39.5 24.0 40.0 54-55 34.95925859069975 39.0 35.0 40.5 26.0 41.0 56-57 34.96611095045216 39.0 35.0 41.0 26.0 41.0 58-59 34.76486959917334 38.5 35.0 41.0 26.0 41.0 60-61 34.419284476232406 38.0 35.0 40.0 25.0 41.0 62-63 33.93875118268518 37.0 34.0 40.0 22.5 41.0 64-65 33.675436680217274 36.5 34.0 39.5 22.0 41.0 66-67 33.386854987600614 36.0 34.0 39.0 22.0 41.0 68-69 33.17257275547688 36.0 34.0 39.0 22.0 41.0 70-71 32.78321891755986 35.0 34.0 38.5 20.5 40.5 72-73 32.38515185954029 35.0 34.0 37.5 20.0 39.5 74-75 32.00567001226124 35.0 33.0 37.0 18.0 39.0 76-77 31.610403638710167 35.0 33.0 36.5 14.5 39.0 78-79 31.278953146326323 35.0 33.0 36.0 9.5 37.0 80-81 30.973187229885756 35.0 33.0 35.5 6.5 37.0 82-83 30.7074870342093 35.0 32.5 35.0 2.0 37.0 84-85 30.439560412572167 35.0 32.0 35.0 2.0 36.0 86-87 30.164147861895525 35.0 32.0 35.0 2.0 36.0 88-89 29.937213707298234 35.0 32.0 35.0 2.0 36.0 90-91 29.68387710757416 35.0 31.5 35.0 2.0 35.0 92-93 29.358033264383018 35.0 31.0 35.0 2.0 35.0 94-95 29.076506607380445 35.0 31.0 35.0 2.0 35.0 96-97 28.816735217893438 35.0 31.0 35.0 2.0 35.0 98-99 28.487725308818213 35.0 30.5 35.0 2.0 35.0 100 27.908268696249607 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4256926032561097 1101 2 0.5429761656994998 1101 3 0.5777225067520533 1101 4 0.4476150092858333 1101 5 0.4845715985417911 1101 6 0.47398829041639345 1101 7 0.5329503794290886 1101 8 0.4908978955541201 1101 9 0.5774087405766153 1101 10-11 0.6164354568391417 1101 12-13 0.5753259516248619 1101 14-15 0.7547753940251667 1101 16-17 0.7486411727048079 1101 18-19 0.748375253625376 1101 20-21 0.7626394807621111 1101 22-23 0.7404669579621128 1101 24-25 0.7815686745004342 1101 26-27 0.7943921577248361 1101 28-29 0.8172763580144604 1101 30-31 0.8418724632522014 1101 32-33 0.8594552176015071 1101 34-35 1.008274695255757 1101 36-37 1.0121250168704705 1101 38-39 0.9751939141393393 1101 40-41 1.0768759983838905 1101 42-43 1.0325699077596795 1101 44-45 1.050043679905059 1101 46-47 1.162368658745656 1101 48-49 1.2777285431262229 1101 50-51 1.172246263404233 1101 52-53 1.2532392729282549 1101 54-55 1.4428182834011452 1101 56-57 1.457653108557082 1101 58-59 1.4769182876527154 1101 60-61 1.4549150104748136 1101 62-63 1.471090290928423 1101 64-65 1.4335917969139942 1101 66-67 1.408547317567887 1101 68-69 1.4195888423286291 1101 70-71 1.359689095860226 1101 72-73 1.3977483291380963 1101 74-75 1.3430554705305227 1101 76-77 1.2854990189465134 1101 78-79 1.3198549527079884 1101 80-81 1.3493148395249648 1101 82-83 1.4000853243482716 1101 84-85 1.3809684881390822 1101 86-87 1.372279933996655 1101 88-89 1.4291037997393516 1101 90-91 1.421861233084801 1101 92-93 1.4102351087718397 1101 94-95 1.3585111109711754 1101 96-97 1.3596388487646571 1101 98-99 1.335377227105134 1101 100 1.314017200144086 1102 1 0.4391641356444538 1102 2 0.5675100548308123 1102 3 0.6130736331433084 1102 4 0.46962858770594806 1102 5 0.4939945938201049 1102 6 0.43501878980328 1102 7 0.500855966999481 1102 8 0.48555961931100455 1102 9 0.5767371377232422 1102 10-11 0.569118674794602 1102 12-13 0.6654796800620986 1102 14-15 0.8270069293643942 1102 16-17 0.8102327236052176 1102 18-19 0.8049934932949938 1102 20-21 0.8009885510751573 1102 22-23 0.8013312249108537 1102 24-25 0.7799720590390322 1102 26-27 0.8327310185115948 1102 28-29 0.8716116272284253 1102 30-31 0.9236193792938678 1102 32-33 0.9661434271635798 1102 34-35 0.9720851839166187 1102 36-37 0.9903281552357015 1102 38-39 1.0035433723916753 1102 40-41 1.1504718398746263 1102 42-43 1.097421045825456 1102 44-45 1.1366132967909337 1102 46-47 1.026811531596877 1102 48-49 1.0556002432460687 1102 50-51 1.1363916222557933 1102 52-53 1.1738539259310983 1102 54-55 1.294048575608464 1102 56-57 1.2655353534481648 1102 58-59 1.3191617664342132 1102 60-61 1.2986904973817772 1102 62-63 1.3621478543044816 1102 64-65 1.310930630172507 1102 66-67 1.2648289744811052 1102 68-69 1.2424652699474734 1102 70-71 1.2308878586882273 1102 72-73 1.2730992915409587 1102 74-75 1.167400968349746 1102 76-77 1.2140917296672704 1102 78-79 1.2348059419849697 1102 80-81 1.278133397686375 1102 82-83 1.2691396778220145 1102 84-85 1.3189320449227147 1102 86-87 1.331957131715864 1102 88-89 1.3210689661530388 1102 90-91 1.3381147603114663 1102 92-93 1.4504280717712454 1102 94-95 1.3128163716731898 1102 96-97 1.3555190493883202 1102 98-99 1.4052460236765896 1102 100 1.3771829827725277 1103 1 0.3817930538268328 1103 2 0.46880377175184407 1103 3 0.5123338931463906 1103 4 0.456753073506583 1103 5 0.44186590415170457 1103 6 0.3716734790079599 1103 7 0.40939000734459796 1103 8 0.3973566985539563 1103 9 0.4657343912649452 1103 10-11 0.5304811484228225 1103 12-13 0.5408996592375459 1103 14-15 0.6398173175086512 1103 16-17 0.6997103241723863 1103 18-19 0.6780113297421408 1103 20-21 0.6591613883057548 1103 22-23 0.7019248120689028 1103 24-25 0.6913366521923265 1103 26-27 0.668898701778005 1103 28-29 0.6316398340741642 1103 30-31 0.6852959030393322 1103 32-33 0.7454710897804944 1103 34-35 0.6686003843438826 1103 36-37 0.5977699064817159 1103 38-39 0.6115239506770607 1103 40-41 0.7111323259375055 1103 42-43 0.7223003315781114 1103 44-45 0.6819590127845245 1103 46-47 0.6419124055448577 1103 48-49 0.6447995338659069 1103 50-51 0.5703420076918633 1103 52-53 0.6411096661113049 1103 54-55 0.7832184245661296 1103 56-57 0.7969878185073185 1103 58-59 0.7945301604880797 1103 60-61 0.7994972469793638 1103 62-63 0.7751877303800541 1103 64-65 0.7880791249578749 1103 66-67 0.7629928607942489 1103 68-69 0.7700430157578921 1103 70-71 0.7626945289966969 1103 72-73 0.767437864134358 1103 74-75 0.7499380209948043 1103 76-77 0.7569351832609179 1103 78-79 0.7304906918904486 1103 80-81 0.7373649977630592 1103 82-83 0.7476174666579354 1103 84-85 0.7253563855144343 1103 86-87 0.6875949697522294 1103 88-89 0.7463512662994134 1103 90-91 0.8573496435067547 1103 92-93 0.8914384279252552 1103 94-95 0.8488730330144421 1103 96-97 0.9353593925145454 1103 98-99 0.9185257922347461 1103 100 0.9531147543162142 1104 1 0.23186517355195235 1104 2 0.29237534447168656 1104 3 0.39424851783704185 1104 4 0.2912961913304599 1104 5 0.37307728186150513 1104 6 0.3382075898322938 1104 7 0.4355961274308413 1104 8 0.3716716673799638 1104 9 0.4853503192089548 1104 10-11 0.47127404054063504 1104 12-13 0.48871599292139223 1104 14-15 0.602541297582782 1104 16-17 0.556070243024422 1104 18-19 0.5901547407183543 1104 20-21 0.5059301272195995 1104 22-23 0.5087837271927995 1104 24-25 0.5122445747406275 1104 26-27 0.5507076429244862 1104 28-29 0.5328052208546907 1104 30-31 0.476997142563917 1104 32-33 0.5509018431607728 1104 34-35 0.5699687472579953 1104 36-37 0.6014152983605427 1104 38-39 0.49814335183904745 1104 40-41 0.5252010892807206 1104 42-43 0.5602982922574569 1104 44-45 0.6949609565120269 1104 46-47 0.6460987484693774 1104 48-49 0.5976997239900683 1104 50-51 0.5118634258694357 1104 52-53 0.5946415145642874 1104 54-55 0.7088952772145944 1104 56-57 0.6611930676337039 1104 58-59 0.5646116270995165 1104 60-61 0.5893778617495684 1104 62-63 0.536622583644565 1104 64-65 0.5630759916266683 1104 66-67 0.5510346881105193 1104 68-69 0.49924778691849525 1104 70-71 0.4450728867062921 1104 72-73 0.5194607215199909 1104 74-75 0.44766778976558186 1104 76-77 0.42797194413995143 1104 78-79 0.44851913616495054 1104 80-81 0.4643206744032753 1104 82-83 0.49801832062345497 1104 84-85 0.46608629236421706 1104 86-87 0.4025229031344182 1104 88-89 0.410338350601954 1104 90-91 0.39999088239404657 1104 92-93 0.44138648897741817 1104 94-95 0.38880579916171953 1104 96-97 0.37639849011515736 1104 98-99 0.45905877718413635 1104 100 0.5957828655016755 1105 1 0.28871199377549317 1105 2 0.3551355755577319 1105 3 0.4156895640338547 1105 4 0.37328513172899136 1105 5 0.42346853381481253 1105 6 0.35729460640867927 1105 7 0.33341976484463487 1105 8 0.36973341212040367 1105 9 0.4973751774923514 1105 10-11 0.49295130205034354 1105 12-13 0.4825385593512479 1105 14-15 0.5879042279128512 1105 16-17 0.5592841234621702 1105 18-19 0.5466665096080092 1105 20-21 0.5583680433487146 1105 22-23 0.4867333397422371 1105 24-25 0.50204383468553 1105 26-27 0.4809930722922715 1105 28-29 0.4743608119939964 1105 30-31 0.5733018314166145 1105 32-33 0.5838369857105619 1105 34-35 0.5883647361888436 1105 36-37 0.6016765698371742 1105 38-39 0.5901361587152181 1105 40-41 0.6122302414806384 1105 42-43 0.6766632825391881 1105 44-45 0.6506311004048797 1105 46-47 0.6249265157673065 1105 48-49 0.719322924244544 1105 50-51 0.6503665999828669 1105 52-53 0.6734637880791752 1105 54-55 0.7940000518154307 1105 56-57 0.7534095921172721 1105 58-59 0.7222767779854351 1105 60-61 0.7812539018689364 1105 62-63 0.8772973816308678 1105 64-65 0.7558349338868311 1105 66-67 0.7287927646442043 1105 68-69 0.7562464803007458 1105 70-71 0.6858383711681242 1105 72-73 0.6867028467721639 1105 74-75 0.7077831765673963 1105 76-77 0.6994744504072479 1105 78-79 0.7168438664770527 1105 80-81 0.7112465643115087 1105 82-83 0.7035903965834684 1105 84-85 0.6744007120692785 1105 86-87 0.5895332740602406 1105 88-89 0.7395196944784956 1105 90-91 0.7308221750864057 1105 92-93 0.7744307607973049 1105 94-95 0.7547074760673915 1105 96-97 0.7642377540003018 1105 98-99 0.8170254872890084 1105 100 0.8286805930456183 1106 1 0.19011303938206936 1106 2 0.24074207928408242 1106 3 0.26432290746277687 1106 4 0.23939096425075945 1106 5 0.3213139523215034 1106 6 0.27235932654136263 1106 7 0.2535143592673208 1106 8 0.2967809523197573 1106 9 0.2531525354558326 1106 10-11 0.348627619534291 1106 12-13 0.3420146323566158 1106 14-15 0.36653194349018037 1106 16-17 0.3904620759431694 1106 18-19 0.37464718929853547 1106 20-21 0.34615798741007353 1106 22-23 0.3981404382045497 1106 24-25 0.42623282044123556 1106 26-27 0.426110701425749 1106 28-29 0.45699349777477494 1106 30-31 0.46363681949272006 1106 32-33 0.3937793554051865 1106 34-35 0.41558700192160813 1106 36-37 0.42831606911225606 1106 38-39 0.4720132992359112 1106 40-41 0.45528238579520774 1106 42-43 0.3653508366445024 1106 44-45 0.5213311137171743 1106 46-47 0.32889712102846147 1106 48-49 0.43474154465602055 1106 50-51 0.405663884762113 1106 52-53 0.3913886720816393 1106 54-55 0.41055058066451267 1106 56-57 0.40586149501150004 1106 58-59 0.37488627453766554 1106 60-61 0.4191799789669375 1106 62-63 0.43790494867069896 1106 64-65 0.3983146107860307 1106 66-67 0.33373700204413836 1106 68-69 0.2970401002142964 1106 70-71 0.3268256586933589 1106 72-73 0.31595050301724825 1106 74-75 0.3581399423314551 1106 76-77 0.4020425018271574 1106 78-79 0.36454315166055196 1106 80-81 0.3791612536755622 1106 82-83 0.3478825410933588 1106 84-85 0.43590891204725324 1106 86-87 0.42295676149200645 1106 88-89 0.39578926217699717 1106 90-91 0.46917333503612113 1106 92-93 0.4931586472500733 1106 94-95 0.4654104297145061 1106 96-97 0.4529770121248866 1106 98-99 0.5247008324996685 1106 100 0.5869221077077178 1107 1 0.23553591202988855 1107 2 0.32241579055326497 1107 3 0.31754721666273156 1107 4 0.2484480749604927 1107 5 0.28817231346039307 1107 6 0.2787494016337462 1107 7 0.31954725580356325 1107 8 0.25893168338033945 1107 9 0.3138519099727546 1107 10-11 0.37353790825436306 1107 12-13 0.37396369580405064 1107 14-15 0.3764828183109401 1107 16-17 0.4036797448241529 1107 18-19 0.44397800209945615 1107 20-21 0.44710658762485167 1107 22-23 0.4481260548641828 1107 24-25 0.4884461300167331 1107 26-27 0.4429642523533275 1107 28-29 0.44978184225291074 1107 30-31 0.4780404808308987 1107 32-33 0.46184256387013534 1107 34-35 0.40006377267435056 1107 36-37 0.40593158375085636 1107 38-39 0.49311136963483193 1107 40-41 0.408590695960811 1107 42-43 0.411115832329493 1107 44-45 0.46203210872456424 1107 46-47 0.4234379873510292 1107 48-49 0.4705858876217306 1107 50-51 0.29706926068966055 1107 52-53 0.40308266495917167 1107 54-55 0.531965989231324 1107 56-57 0.5326612814155496 1107 58-59 0.4908465556480692 1107 60-61 0.4871310330760039 1107 62-63 0.5112964616089286 1107 64-65 0.5171046859638437 1107 66-67 0.4673648399162502 1107 68-69 0.43212398431911225 1107 70-71 0.4105260967353317 1107 72-73 0.3961066025360509 1107 74-75 0.3570565234730836 1107 76-77 0.37445555859271096 1107 78-79 0.37808713838076713 1107 80-81 0.4367192840769505 1107 82-83 0.5029240803878317 1107 84-85 0.576408364845669 1107 86-87 0.5041067425074601 1107 88-89 0.44516800347788177 1107 90-91 0.5049878220439474 1107 92-93 0.5470951362284815 1107 94-95 0.49896452932044966 1107 96-97 0.5912769107519331 1107 98-99 0.6022483420703608 1107 100 0.6896105535710078 1108 1 0.12265903167339332 1108 2 0.13730466642687134 1108 3 0.12278416163130856 1108 4 0.1557322182664862 1108 5 0.23447473338826796 1108 6 0.208416576248176 1108 7 0.26399680083151367 1108 8 0.1596900697949195 1108 9 0.2550375652324064 1108 10-11 0.23934214979408353 1108 12-13 0.25251911030209584 1108 14-15 0.27805360456060413 1108 16-17 0.32195966889229766 1108 18-19 0.3134681187515582 1108 20-21 0.328436367786189 1108 22-23 0.28031861631389887 1108 24-25 0.32576614097771284 1108 26-27 0.3274727073619701 1108 28-29 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-1.098959169299441 2316 76-77 -1.0786210668455105 2316 78-79 -1.014467182653064 2316 80-81 -0.9423450786840277 2316 82-83 -0.9456970279070696 2316 84-85 -0.9411056890570215 2316 86-87 -0.944063123603005 2316 88-89 -0.9348625838970506 2316 90-91 -0.9199206394305683 2316 92-93 -0.853056982306402 2316 94-95 -0.716032755476288 2316 96-97 -0.78344719441397 2316 98-99 -0.8578417922803325 2316 100 -0.9666092957225736 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22713.0 3 4038.0 4 3956.0 5 4491.0 6 5451.0 7 6419.0 8 7916.0 9 9732.0 10 12683.0 11 18298.0 12 27703.0 13 38888.0 14 41380.0 15 50077.0 16 66235.0 17 91040.0 18 91053.0 19 52690.0 20 43887.0 21 40330.0 22 41092.0 23 47083.0 24 52975.0 25 58145.0 26 64955.0 27 75972.0 28 89899.0 29 107889.0 30 132040.0 31 161926.0 32 205640.0 33 272594.0 34 390155.0 35 615864.0 36 1105257.0 37 1974189.0 38 1729841.0 39 378506.0 40 543.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.23656699068849 17.731180116160033 13.214663707445782 23.8175891857057 2 14.183610419950679 22.217230336809287 37.17778766294346 26.421371580296572 3 19.47928615963558 28.02615057784365 29.24488949142313 23.249673771097633 4 13.270005129022463 18.659829942274154 34.42277912156276 33.647385807140616 5 13.080332904031918 36.98019465386711 33.773108618513305 16.166363823587666 6 30.606303299579928 33.45039858134627 18.830141891196458 17.113156227877344 7 24.49154505956387 32.57046029665836 22.740995564685925 20.196999079091842 8 27.6931557001282 31.04263337091233 21.757342136548612 19.506868792410852 9 28.212746216386165 14.459895969846762 19.848716973748814 37.478640840018265 10-11 24.4514692347226 26.2792888193547 26.015397728297017 23.253844217625673 12-13 27.357053476625225 24.06301372644762 26.35613321112355 22.223799585803604 14-15 26.125649093062204 23.02648837132938 25.42397738672486 25.423885148883556 16-17 22.607230755704535 26.38121599612412 28.257538262333924 22.75401498583742 18-19 24.22178473881199 25.24894697330463 29.755026781954353 20.774241505929027 20-21 23.77145751954281 24.878826551398024 29.085944580999897 22.263771348059265 22-23 22.470759928217454 25.99731737946965 26.524493518537877 25.007429173775016 24-25 22.484764245450496 26.972984237186324 26.022832380928246 24.519419136434937 26-27 23.802993051679575 24.8131764387513 26.509538372595422 24.874292136973704 28-29 22.953060886446252 24.305138282837536 29.231285804254505 23.51051502646171 30-31 24.713033256021223 24.72697700303218 28.0004285271588 22.559561213787795 32-33 21.406086476673174 25.79875684121297 28.524141519455675 24.271015162658177 34-35 20.982810659529378 26.56481675087522 29.124082075963713 23.328290513631693 36-37 23.508924898405972 25.97055896472727 27.362424485859798 23.158091651006956 38-39 24.41145705374631 24.666478299166837 26.900384522877374 24.021680124209478 40-41 22.77047052022759 26.118229783700762 27.107583903220767 24.003715792850876 42-43 25.006009299298498 25.15230317148451 26.69019223367498 23.15149529554201 44-45 24.918548878171052 24.674705619310007 26.97814432797078 23.42860117454816 46-47 23.173311101151526 25.27167719196673 29.16852332808088 22.386488378800863 48-49 24.14297916123093 25.973165100353956 27.87810575441464 22.005749984000474 50-51 22.349123192690936 26.61565258725478 26.940691734776816 24.09453248527747 52-53 21.86798024663493 27.112741960497083 24.899816046926073 26.11946174594192 54-55 22.87067703447314 26.934579485185207 24.509645635550346 25.68509784479131 56-57 23.9534895724077 24.85555277415971 25.123119777432624 26.067837875999967 58-59 23.422124481927675 24.625175887044808 27.32704430349714 24.62565532753038 60-61 23.950935544292097 25.38792149883733 26.386490897739062 24.274652059131512 62-63 21.396247570289226 25.826439678151054 27.25020655768091 25.52710619387881 64-65 21.01171701317886 26.141851619247042 27.538533554650733 25.30789781292337 66-67 23.56260399211725 25.569676439467248 26.116065282465208 24.7516542859503 68-69 24.224403304074958 24.51105874214893 25.864463261736358 25.40007469203975 70-71 22.555357157677335 25.62962398291203 26.25637548285839 25.558643376552237 72-73 24.3591868991426 24.969263427888134 26.095279007643775 24.576270665325495 74-75 24.81102870968371 24.26936360917801 26.34370305169771 24.575904629440572 76-77 23.209647542156997 25.377937920237333 27.97202921960777 23.440385317997897 78-79 23.843411314830785 25.483037949680803 27.31601689806486 23.357533837423556 80-81 22.371274931020853 25.6850271206021 27.14949845891214 24.794199489464905 82-83 22.243330403804638 26.365247981683837 25.212593714946713 26.178827899564812 84-85 22.898225374111593 26.5482291285572 24.68953563749902 25.864009859832187 86-87 23.54602652257036 24.720901156275424 25.48075018777574 26.25232213337847 88-89 23.613623980212463 24.188013583637645 26.799481450413698 25.398880985736195 90-91 24.388947819879693 25.1234142070197 25.851034415732798 24.636603557367806 92-93 22.64509689905448 25.56662556362012 26.41042319010818 25.377854347217223 94-95 22.1141937192635 25.919856267943324 26.947345541300326 25.01860447149285 96-97 24.362822862775086 25.2542544450053 25.491995820611603 24.890926871608013 98-99 24.946191240961483 24.573060063339664 25.142418091453656 25.338330604245197 100 24.089845312564524 25.380154373092758 25.963266279716947 24.566734034625775 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2761.0 1 2843.5 2 2164.5 3 1323.5 4 1085.0 5 1016.5 6 1375.0 7 2419.0 8 3707.0 9 3943.5 10 3085.0 11 2616.5 12 2880.0 13 3167.0 14 3558.0 15 4199.5 16 4819.0 17 5524.5 18 6575.0 19 7828.0 20 9418.0 21 11505.0 22 13991.0 23 17052.0 24 20792.5 25 25902.0 26 32828.5 27 40227.0 28 47111.0 29 55966.0 30 65482.5 31 73814.0 32 82126.5 33 91329.5 34 99886.5 35 106004.0 36 113048.0 37 121547.5 38 127076.0 39 133639.5 40 140412.0 41 144143.5 42 150003.5 43 163573.0 44 183109.0 45 203024.0 46 230948.0 47 273233.0 48 385257.5 49 564222.0 50 842259.5 51 788699.5 52 443733.0 53 317752.5 54 274678.5 55 240264.0 56 219550.0 57 196194.5 58 180087.0 59 165327.0 60 140917.0 61 114398.5 62 90832.5 63 71356.5 64 52404.0 65 40514.5 66 32984.5 67 25200.0 68 19213.0 69 15841.0 70 13508.5 71 11399.5 72 9741.0 73 9781.0 74 7995.5 75 6046.0 76 4881.5 77 3655.5 78 2752.0 79 2053.0 80 1596.5 81 1346.5 82 1124.0 83 917.5 84 685.5 85 445.0 86 302.0 87 184.5 88 138.5 89 120.0 90 102.0 91 74.0 92 64.0 93 62.0 94 53.0 95 38.5 96 23.0 97 21.0 98 20.0 99 15.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11569899840916946 2 0.3730316465372267 3 0.3824255898383321 4 0.3792942754046303 5 0.3061197549715756 6 0.13910403884303457 7 0.15277130537130942 8 0.12839617144621907 9 0.2094296771246429 10-11 0.20981648655468843 12-13 0.22315220214292425 14-15 0.15216960181346084 16-17 0.13753224179396073 18-19 0.1559148994694571 20-21 0.15646134453729918 22-23 0.16497729183052345 24-25 0.17776042251869426 26-27 0.17047244167005893 28-29 0.17849106255322467 30-31 0.221512866939398 32-33 0.21849206948570923 34-35 0.1333510160501354 36-37 0.09192556804192768 38-39 0.08556470185895701 40-41 0.08501825679111494 42-43 0.07560589399334074 44-45 0.09812679858710181 46-47 0.14477110398481252 48-49 0.2247792576820046 50-51 0.1802409147367639 52-53 0.1121563152165304 54-55 0.06004755914039893 56-57 0.07544625835554417 58-59 0.11129059887309518 60-61 0.08724701588804384 62-63 0.09581822167127461 64-65 0.10587526685245799 66-67 0.10208085053867819 68-69 0.0917352332430164 70-71 0.12694103120938116 72-73 0.15167841523562528 74-75 0.1955106774752273 76-77 0.19549839781078143 78-79 0.15707532775959365 80-81 0.09297547935205124 82-83 0.13308086343232586 84-85 0.16188895622238228 86-87 0.17396600620491445 88-89 0.1617845790745922 90-91 0.18324329269378384 92-93 0.10192735473310457 94-95 0.34246142189918516 96-97 0.3438735833104624 98-99 0.3837456537662652 100 0.38533587031200783 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8143545.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.00318485530528 #Duplication Level Percentage of deduplicated Percentage of total 1 77.30108113159332 25.511818701009254 2 12.416715240099396 8.195822967293735 3 3.837782671840316 3.7997715285970006 4 1.7408718992239318 2.2981726839797525 5 1.0183165589856702 1.6803844818711233 6 0.6376334109358228 1.2626359998620278 7 0.43735162717375603 1.0103797618868817 8 0.3263372208254304 0.8616134099254608 9 0.24596767873936312 0.7305945092878989 >10 1.6452708486417877 10.58406507055999 >50 0.1934129047871618 4.459166076254435 >100 0.1661760357180081 11.16686438110419 >500 0.020878601761726196 4.681826585494767 >1k 0.010101297361437651 5.917728188830406 >5k 6.383719521883036E-4 1.5415928492761635 >10k+ 0.001464500360902579 16.29756280476698 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 172553 2.1188929391315456 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 161431 1.982318511164364 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 75594 0.9282689541225596 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 61228 0.7518592946929132 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 58107 0.7135344619572925 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 55952 0.687071785076401 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 53043 0.6513502412033089 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 51645 0.6341832703079556 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 42439 0.5211366794190982 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 38024 0.4669219608904967 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 34509 0.4237589403631956 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 34102 0.41876111693371865 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 33000 0.40522892671434857 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30658 0.3764699525820757 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 26901 0.3303352532588694 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 24720 0.30355330510238476 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 24129 0.29629602341486416 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 21691 0.26635820149578593 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 21402 0.2628093784709239 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20604 0.25301020624310416 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 19892 0.244267085157631 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 19209 0.23588007434108857 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 16881 0.2072930155110581 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 16464 0.20217239543712226 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 16029 0.1968307414031604 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 15658 0.19227498589373546 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 15372 0.18876300186221112 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15071 0.18506682286399842 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 14932 0.1833599495060198 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 14902 0.18299155957264313 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 13996 0.17186618358466735 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 13534 0.16619297861066648 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 13042 0.16015138370328894 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 12507 0.15358176322473813 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 12077 0.1483015075130057 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 12031 0.14773664294849478 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 10889 0.13371326615128915 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 10788 0.13247302004225434 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 10043 0.12332467003006677 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 9957 0.12226861888772027 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 9761 0.11986180465632595 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 9658 0.11859699921839936 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9588 0.1177374227071871 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 8766 0.10764353853266605 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 8699 0.10682080101479148 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.9647463113422963E-4 0.0 0.0 1.2279664445889352E-5 0.0 2 2.578729533636764E-4 1.2279664445889352E-5 0.0 1.2279664445889352E-5 0.0 3 2.578729533636764E-4 2.4559328891778703E-5 0.0 1.2279664445889352E-5 0.0 4 3.192712755931231E-4 4.9118657783557406E-5 0.0 2.4559328891778703E-5 0.0 5 0.001743712351316288 4.9118657783557406E-5 0.0 4.9118657783557406E-5 0.0 6 0.0030453567825805593 4.9118657783557406E-5 0.0 4.9118657783557406E-5 0.0 7 0.003364628058173682 4.9118657783557406E-5 0.0 6.139832222944676E-5 0.0 8 0.0036593400048750267 7.367798667533611E-5 0.0 8.595765112122546E-5 0.0 9 0.003966331616022261 1.2279664445889353E-4 0.0 9.823731556711481E-5 0.0 10-11 0.006753815445239144 1.2279664445889353E-4 0.0 1.9647463113422963E-4 0.0 12-13 0.014054075958320363 1.2279664445889353E-4 0.0 2.8857211447839976E-4 0.0 14-15 0.022508624929315182 1.8419496668834026E-4 0.0 4.17508591160238E-4 0.0 16-17 0.02585483349082003 2.2103396002600833E-4 0.0 6.201230545174123E-4 2.4559328891778703E-5 18-19 0.027807300137716436 2.3945345669484236E-4 0.0 8.043180212057525E-4 2.4559328891778703E-5 20-21 0.0347698698785357 2.578729533636764E-4 0.0 0.001252525773480714 2.4559328891778703E-5 22-23 0.037643311358873804 2.8243228225545506E-4 0.0 0.003051496614803504 2.4559328891778703E-5 24-25 0.0543252355086145 3.3155094003901246E-4 0.0 0.006673997626340862 2.4559328891778703E-5 26-27 0.10707867396815515 3.4383060448490185E-4 0.0 0.012248965284774628 2.4559328891778703E-5 28-29 0.11817949062723912 3.4383060448490185E-4 0.0 0.036845133169891 2.4559328891778703E-5 30-31 0.11995390213967014 3.9294926226845925E-4 0.0 0.10715849178705343 2.4559328891778703E-5 32-33 0.12138448304761623 3.9294926226845925E-4 0.0 0.22188125687277468 2.4559328891778703E-5 34-35 0.12267998764665757 4.113687589372933E-4 0.0 0.36994331092908556 2.4559328891778703E-5 36-37 0.12480436959579642 4.3592808782907195E-4 0.0 0.5909158726328645 2.4559328891778703E-5 38-39 0.1270269888605024 4.54347584497906E-4 0.0 0.9149639376954386 2.4559328891778703E-5 40-41 0.12913295131297242 5.034662422814634E-4 0.0 1.3698518274289637 2.4559328891778703E-5 42-43 0.1334431135334796 5.464450678420761E-4 0.0 1.7019369328713725 2.4559328891778703E-5 44-45 0.13883388622522502 6.078433900715229E-4 0.0 2.0459333128262935 2.4559328891778703E-5 46-47 0.1418117048533532 6.385425511862462E-4 0.0 2.4459372423189163 2.4559328891778703E-5 48-49 0.14329754425130578 6.385425511862462E-4 0.0 2.811650208846393 2.4559328891778703E-5 50-51 0.14556928217379533 7.36779866753361E-4 0.0 3.207172060816266 2.4559328891778703E-5 52-53 0.14651481633612878 7.36779866753361E-4 0.0 3.6672174096170647 2.4559328891778703E-5 54-55 0.14936369848757514 7.490595311992504E-4 0.0 4.077892367513165 2.4559328891778703E-5 56-57 0.15555264936830338 7.613391956451398E-4 0.0 4.5127275652065535 3.069916111472338E-5 58-59 0.15779982796190112 7.613391956451398E-4 0.0 4.969831934372562 3.6838993337668056E-5 60-61 0.15926110803096194 7.674790278680845E-4 0.0 5.384374986569117 3.6838993337668056E-5 62-63 0.1602434811866331 7.736188600910291E-4 0.0 5.81133891935269 3.6838993337668056E-5 64-65 0.16102937971117 8.595765112122546E-4 0.0 6.280986965750174 3.6838993337668056E-5 66-67 0.16190737571905112 8.595765112122546E-4 0.0 6.753637390104679 3.6838993337668056E-5 68-69 0.16303096501584996 8.90275672326978E-4 0.0 7.310016706483479 3.6838993337668056E-5 70-71 0.16428963062155363 9.148350012187568E-4 0.0 7.999212873509019 3.6838993337668056E-5 72-73 0.1682007037475694 9.393943301105353E-4 0.0 8.56247494180974 5.525849000650208E-5 74-75 0.1720073997257951 9.823731556711482E-4 0.0 9.098267400745009 6.139832222944676E-5 76-77 0.17316782801593164 0.0010437714779005947 0.0 9.687924607772167 6.139832222944676E-5 78-79 0.17400898503047507 0.0010683308067923735 0.0 10.25873866970711 6.139832222944676E-5 80-81 0.1754764049317588 0.001080610471238263 0.0 10.885934810945358 6.753815445239143E-5 82-83 0.17608424832183034 0.0010928901356841523 0.0 11.624360152734466 7.367798667533611E-5 84-85 0.17829458792209044 0.0011235892967988758 0.0 12.307127915422583 7.367798667533611E-5 86-87 0.18308365705598728 0.0011604282901365437 0.0 12.983332197464371 7.367798667533611E-5 88 0.18446511930614984 0.0012648054379266033 0.0 13.537151203806205 7.367798667533611E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 8055 0.0 25.49857 1 ATCTACA 9485 0.0 21.797375 2 TCTACAC 10600 0.0 21.549356 3 AGTACTT 34615 0.0 21.089653 12-13 TACACTC 10700 0.0 20.624239 5 GAGTACT 33855 0.0 20.03576 12-13 GTATAAG 2170 0.0 19.103714 1 CTACACT 12855 0.0 19.08157 4 TACTTTT 40220 0.0 18.273956 14-15 GTACTTT 38135 0.0 17.868702 14-15 TATCACG 400 2.5465852E-11 17.660715 2 CACTCTT 12940 0.0 17.04554 7 GTGATCG 1410 0.0 17.008018 8 CATGGGG 16510 0.0 16.710869 4 ACTTTTT 42230 0.0 16.34786 16-17 CATGGGT 8995 0.0 16.27825 4 GTCCTAC 1940 0.0 15.783615 1 ACTCTTT 15180 0.0 15.736009 8 GTACTAG 1895 0.0 15.6612425 1 AGTACTC 2800 0.0 15.460325 5 >>END_MODULE