##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765425_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8143545
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50420596926769	34.0	31.0	34.0	31.0	34.0
2	32.6279914951044	34.0	31.0	34.0	31.0	34.0
3	32.669634784359886	34.0	31.0	34.0	31.0	34.0
4	36.16924287886909	37.0	35.0	37.0	35.0	37.0
5	36.11206876120903	37.0	35.0	37.0	35.0	37.0
6	36.17974039561395	37.0	37.0	37.0	35.0	37.0
7	36.13491507691061	37.0	36.0	37.0	35.0	37.0
8	36.15317174522889	37.0	37.0	37.0	35.0	37.0
9	37.88116268774839	39.0	38.0	39.0	35.0	39.0
10-11	37.83819423850424	39.0	38.0	39.0	35.0	39.0
12-13	37.80635662969874	39.0	38.0	39.0	35.0	39.0
14-15	39.15584717712004	40.5	38.5	41.0	36.0	41.0
16-17	39.12391630426307	40.0	38.5	41.0	36.0	41.0
18-19	39.16727586081983	40.0	39.0	41.0	36.0	41.0
20-21	39.15588941916573	40.0	39.0	41.0	36.0	41.0
22-23	39.105851812693366	40.0	39.0	41.0	35.5	41.0
24-25	39.05068259584739	40.0	39.0	41.0	35.0	41.0
26-27	38.90146238523886	40.0	38.0	41.0	35.0	41.0
28-29	38.766078654934674	40.0	38.0	41.0	35.0	41.0
30-31	38.57267080859748	40.0	38.0	41.0	34.5	41.0
32-33	38.26120737344731	40.0	38.0	41.0	34.0	41.0
34-35	38.17533973226648	40.0	38.0	41.0	34.0	41.0
36-37	38.07856265299694	40.0	38.0	41.0	34.0	41.0
38-39	37.90769744625958	40.0	37.0	41.0	33.0	41.0
40-41	37.6616669398892	40.0	37.0	41.0	33.0	41.0
42-43	37.38527926105891	40.0	37.0	41.0	33.0	41.0
44-45	37.025006308677604	40.0	36.0	41.0	32.0	41.0
46-47	36.62639520012476	39.5	36.0	41.0	31.0	41.0
48-49	36.2816013173624	39.0	35.5	41.0	30.5	41.0
50-51	35.99946577319828	39.0	35.0	41.0	29.5	41.0
52-53	35.86489170256934	39.0	35.0	41.0	29.5	41.0
54-55	35.851173536831936	39.0	35.0	41.0	29.5	41.0
56-57	35.67245038862068	39.0	35.0	41.0	29.0	41.0
58-59	35.54799678763978	39.0	35.0	41.0	29.0	41.0
60-61	35.21657343331436	38.0	35.0	40.5	28.0	41.0
62-63	34.834521513665116	37.0	35.0	40.0	28.0	41.0
64-65	34.629780765010814	37.0	35.0	40.0	28.0	41.0
66-67	34.26408474442027	36.5	35.0	39.0	28.0	41.0
68-69	33.93534560194608	36.0	34.0	39.0	27.0	41.0
70-71	33.53008309034948	35.0	34.0	38.5	26.0	40.5
72-73	33.130768111430584	35.0	34.0	37.0	26.0	39.5
74-75	32.77974843879416	35.0	34.0	37.0	26.0	39.0
76-77	31.329225601381218	34.5	31.5	35.5	24.0	37.0
78-79	32.05596518469537	35.0	33.0	36.0	25.0	37.0
80-81	32.016259872082735	35.0	34.0	35.5	25.0	37.0
82-83	31.859863364173712	35.0	34.0	35.0	25.0	36.5
84-85	31.670976767488852	35.0	33.5	35.0	24.5	36.0
86-87	31.482404468815485	35.0	33.0	35.0	24.0	36.0
88-89	31.330160022447227	35.0	33.0	35.0	23.5	36.0
90-91	31.186315357746537	35.0	33.0	35.0	23.0	35.0
92-93	30.99538843341567	35.0	33.0	35.0	20.0	35.0
94-95	30.91183213207516	35.0	33.0	35.0	19.0	35.0
96-97	30.812178418612532	35.0	33.0	35.0	17.0	35.0
98-99	30.673388186594416	35.0	33.0	35.0	2.0	35.0
100	30.34019447304583	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4626880501437398
1101	2	0.4106762764756908
1101	3	0.39837455474828687
1101	4	0.2734296909669567
1101	5	0.32145521382403786
1101	6	0.3261699508257152
1101	7	0.2774508104297695
1101	8	0.29623450092514503
1101	9	0.3744071288087767
1101	10-11	0.41499458247725585
1101	12-13	0.41114892840867157
1101	14-15	0.5782538971950189
1101	16-17	0.5529743807984389
1101	18-19	0.5057304156906071
1101	20-21	0.5124404332193535
1101	22-23	0.5394034866147592
1101	24-25	0.5778486366484827
1101	26-27	0.5315764673312557
1101	28-29	0.5454333005280674
1101	30-31	0.6314120562750887
1101	32-33	0.6926137213802619
1101	34-35	0.6282111731622706
1101	36-37	0.7315805282124472
1101	38-39	0.7328256666187727
1101	40-41	0.7403022725843513
1101	42-43	0.7617751881363333
1101	44-45	0.9106269988366407
1101	46-47	1.0059490151895503
1101	48-49	1.0575018331722958
1101	50-51	1.0701845906931169
1101	52-53	1.022470312818207
1101	54-55	1.1462613274026836
1101	56-57	1.177461035676842
1101	58-59	1.2878314549770096
1101	60-61	1.274908736473492
1101	62-63	1.2659983706263702
1101	64-65	1.2076665631373302
1101	66-67	1.2096262989373727
1101	68-69	1.2149834544214215
1101	70-71	1.1811760670185976
1101	72-73	1.2221359031572945
1101	74-75	1.2368882505646823
1101	76-77	1.2661307888562696
1101	78-79	1.2232417955688746
1101	80-81	1.2328943673631372
1101	82-83	1.2160298826621379
1101	84-85	1.2020864897790347
1101	86-87	1.2316112789661027
1101	88-89	1.2700708586875784
1101	90-91	1.2939240638507812
1101	92-93	1.3414219059344497
1101	94-95	1.2965050402962142
1101	96-97	1.3622368389477693
1101	98-99	1.4037300171335438
1101	100	1.4928110754349824
1102	1	0.4226519890521132
1102	2	0.4004422200762221
1102	3	0.40834177796701
1102	4	0.3023551073225619
1102	5	0.3003248332446944
1102	6	0.2989261195373345
1102	7	0.3319865565819242
1102	8	0.2842387958763837
1102	9	0.4057804693121696
1102	10-11	0.40231519122031756
1102	12-13	0.3947695321457658
1102	14-15	0.49367560355187123
1102	16-17	0.5637720280329859
1102	18-19	0.5463480223014585
1102	20-21	0.5028691556444258
1102	22-23	0.555306146833459
1102	24-25	0.5403295588028811
1102	26-27	0.5790182961987895
1102	28-29	0.6112455877008927
1102	30-31	0.6110548514112466
1102	32-33	0.6779015782355629
1102	34-35	0.6648692438393553
1102	36-37	0.7251416105248936
1102	38-39	0.6837817257261776
1102	40-41	0.7492131675639229
1102	42-43	0.8062633635742884
1102	44-45	0.8835326990638706
1102	46-47	0.8734025058572428
1102	48-49	0.9194552850914874
1102	50-51	0.8754242604014237
1102	52-53	0.917127966392421
1102	54-55	0.991431737683456
1102	56-57	1.0362457072962243
1102	58-59	0.9729894475665475
1102	60-61	1.0419362389158309
1102	62-63	0.9852922039271306
1102	64-65	1.0786426356418275
1102	66-67	1.1116989758647122
1102	68-69	1.0916110732273694
1102	70-71	1.033085900298289
1102	72-73	1.0319230707616995
1102	74-75	1.0330091779630735
1102	76-77	1.0777195006225604
1102	78-79	1.0662616639656974
1102	80-81	1.0349280414016562
1102	82-83	1.0362535823546146
1102	84-85	1.0330521215381623
1102	86-87	1.0713080885152557
1102	88-89	1.0429873271826828
1102	90-91	1.0559212793188948
1102	92-93	1.0998070841280487
1102	94-95	1.2045181056734329
1102	96-97	1.2032929804854682
1102	98-99	1.229682794201917
1102	100	1.2685510287387203
1103	1	0.3829033424160784
1103	2	0.33904421222385395
1103	3	0.3357949823714321
1103	4	0.2893172821234913
1103	5	0.30145290112853473
1103	6	0.2874727036952649
1103	7	0.30476589068035054
1103	8	0.2640791518742205
1103	9	0.3668173341175347
1103	10-11	0.3687190453578637
1103	12-13	0.34186549960771373
1103	14-15	0.46231982573507935
1103	16-17	0.4652758310788201
1103	18-19	0.45929415015224606
1103	20-21	0.5126265438197422
1103	22-23	0.5054696977982545
1103	24-25	0.5325435434808625
1103	26-27	0.5625289273761993
1103	28-29	0.5517988729905099
1103	30-31	0.5237926737099272
1103	32-33	0.5142568223096049
1103	34-35	0.511982300508599
1103	36-37	0.568501906448347
1103	38-39	0.5749441771122505
1103	40-41	0.574927172922294
1103	42-43	0.6391214127153475
1103	44-45	0.6579128795650249
1103	46-47	0.6113262542691018
1103	48-49	0.5916365570021895
1103	50-51	0.6902001648165097
1103	52-53	0.6769004787092641
1103	54-55	0.7463504088459132
1103	56-57	0.7389555415665257
1103	58-59	0.6141534649984806
1103	60-61	0.6871943936384213
1103	62-63	0.69698298941627
1103	64-65	0.6859138595105208
1103	66-67	0.6538234642396787
1103	68-69	0.6346433318842557
1103	70-71	0.5668818564503795
1103	72-73	0.6825402987764306
1103	74-75	0.7257960522505229
1103	76-77	0.6478010182437082
1103	78-79	0.7365478273112345
1103	80-81	0.7027764934704379
1103	82-83	0.7812880370797508
1103	84-85	0.8323707671633471
1103	86-87	0.7816372293078366
1103	88-89	0.7990107686152221
1103	90-91	0.8226408783356334
1103	92-93	0.7883041512030324
1103	94-95	0.8562093622634812
1103	96-97	0.8581770613418307
1103	98-99	0.8478496427015223
1103	100	0.8937372594800053
1104	1	0.3176491177296725
1104	2	0.17071804837718219
1104	3	0.2350899297420881
1104	4	0.18532085093463024
1104	5	0.21081295805957012
1104	6	0.20202134467923827
1104	7	0.24763538478012492
1104	8	0.2148457027669508
1104	9	0.2892602703144149
1104	10-11	0.2453285685604456
1104	12-13	0.29013515727800154
1104	14-15	0.3965360149670474
1104	16-17	0.3950885747086019
1104	18-19	0.37602443937464614
1104	20-21	0.3622824084598406
1104	22-23	0.3324901653550043
1104	24-25	0.3635502456232089
1104	26-27	0.418570521020321
1104	28-29	0.4200152109229336
1104	30-31	0.4540430215628035
1104	32-33	0.4483545822433257
1104	34-35	0.4684456723978343
1104	36-37	0.5383254839690892
1104	38-39	0.5204725417257876
1104	40-41	0.5016728632641971
1104	42-43	0.6148277930274091
1104	44-45	0.609130417722632
1104	46-47	0.5414081457390054
1104	48-49	0.5865327322138185
1104	50-51	0.5852722314343168
1104	52-53	0.566852682131497
1104	54-55	0.6065511544725268
1104	56-57	0.5741479493456865
1104	58-59	0.6188564358213284
1104	60-61	0.7073608319436602
1104	62-63	0.6986553385649614
1104	64-65	0.670191759435113
1104	66-67	0.6562447326546348
1104	68-69	0.6343275558271557
1104	70-71	0.6216836068672649
1104	72-73	0.6053313002740595
1104	74-75	0.5666305804982983
1104	76-77	0.5296410802730414
1104	78-79	0.6152014508229016
1104	80-81	0.5688721503547356
1104	82-83	0.5807720464691251
1104	84-85	0.5672391141452593
1104	86-87	0.5842643416510391
1104	88-89	0.6015444120952402
1104	90-91	0.5853957668633925
1104	92-93	0.6386463746998317
1104	94-95	0.6273273773247148
1104	96-97	0.6808128832836466
1104	98-99	0.6624645616066651
1104	100	0.7052105624359584
1105	1	0.27231596491875365
1105	2	0.19669265460182572
1105	3	0.2653367898020704
1105	4	0.2056952873987683
1105	5	0.21198345496920723
1105	6	0.2191580943124407
1105	7	0.1967421937296976
1105	8	0.1927575531520631
1105	9	0.24039807791310608
1105	10-11	0.22828837238118638
1105	12-13	0.2910105260796527
1105	14-15	0.33041988090658236
1105	16-17	0.3517142115816867
1105	18-19	0.35252095064883804
1105	20-21	0.3371913119033465
1105	22-23	0.3296010256441022
1105	24-25	0.3462403159792089
1105	26-27	0.35965385511305925
1105	28-29	0.394445079050449
1105	30-31	0.4008833889779808
1105	32-33	0.38453351293443205
1105	34-35	0.433357869507482
1105	36-37	0.4179218775131517
1105	38-39	0.43328928587389015
1105	40-41	0.45015742534329206
1105	42-43	0.4519277698979778
1105	44-45	0.4706348550590036
1105	46-47	0.49852471678520516
1105	48-49	0.4764498785016258
1105	50-51	0.4655779391971677
1105	52-53	0.4918954281898422
1105	54-55	0.5677804600297094
1105	56-57	0.60151507768974
1105	58-59	0.5461208486483144
1105	60-61	0.577457901795853
1105	62-63	0.5280740694142878
1105	64-65	0.5283529737607822
1105	66-67	0.5570702668556038
1105	68-69	0.5971219125923781
1105	70-71	0.5422376631321129
1105	72-73	0.5091296914548451
1105	74-75	0.5321371476519872
1105	76-77	0.5134369285179154
1105	78-79	0.5942175776364209
1105	80-81	0.6139656162678904
1105	82-83	0.6415520161187978
1105	84-85	0.6092714406755846
1105	86-87	0.5890574807578766
1105	88-89	0.6255968625351152
1105	90-91	0.6492745686285204
1105	92-93	0.6167271176884235
1105	94-95	0.6783180244411504
1105	96-97	0.7087054707122569
1105	98-99	0.7334227320467228
1105	100	0.8087723651084708
1106	1	0.1575690213185723
1106	2	0.12365439086487129
1106	3	0.1558357562356747
1106	4	0.1052049360530134
1106	5	0.16810166433132423
1106	6	0.14182672651352846
1106	7	0.14515417521018747
1106	8	0.12171984465106789
1106	9	0.17293300631516217
1106	10-11	0.1901859073185861
1106	12-13	0.17972645073768945
1106	14-15	0.2538993458498098
1106	16-17	0.21551820333115046
1106	18-19	0.19565339761776102
1106	20-21	0.20796694575704322
1106	22-23	0.22434876464131293
1106	24-25	0.23981018633977413
1106	26-27	0.2682734660572592
1106	28-29	0.2828325249740473
1106	30-31	0.22639890005526553
1106	32-33	0.31027969075617534
1106	34-35	0.2693841700362327
1106	36-37	0.30748591513351187
1106	38-39	0.24229398816089542
1106	40-41	0.3532370847411741
1106	42-43	0.37967963480846834
1106	44-45	0.36786706132716773
1106	46-47	0.29587237930095256
1106	48-49	0.3004079164254989
1106	50-51	0.2970220493207236
1106	52-53	0.363958032746325
1106	54-55	0.3902915242722287
1106	56-57	0.32631121543965946
1106	58-59	0.37630120588823246
1106	60-61	0.39159231258590665
1106	62-63	0.34507890456728774
1106	64-65	0.3551500747249321
1106	66-67	0.37146497260818023
1106	68-69	0.3385677644940799
1106	70-71	0.3888649500431285
1106	72-73	0.37739402623758167
1106	74-75	0.38395383799852567
1106	76-77	0.2295239988573563
1106	78-79	0.2862875527863693
1106	80-81	0.32013734467078336
1106	82-83	0.32160063813520523
1106	84-85	0.35997679336350075
1106	86-87	0.3897555364169101
1106	88-89	0.3829802896477723
1106	90-91	0.4196199797466278
1106	92-93	0.44341215312213933
1106	94-95	0.44514925680412176
1106	96-97	0.5138533326426433
1106	98-99	0.5321109003681563
1106	100	0.5610379456063121
1107	1	0.151489146119836
1107	2	0.08773070153380047
1107	3	0.14330578890910317
1107	4	0.11942012498249
1107	5	0.17246032906426478
1107	6	0.14462282774344004
1107	7	0.07772514268479824
1107	8	0.09671905744236398
1107	9	0.11036701656254877
1107	10-11	0.15081254283619927
1107	12-13	0.21353779672177353
1107	14-15	0.25159550940956876
1107	16-17	0.20643392934444904
1107	18-19	0.2231101081569733
1107	20-21	0.2414208364414563
1107	22-23	0.21378555282004896
1107	24-25	0.2598157529426999
1107	26-27	0.26971912296583866
1107	28-29	0.29933750721181696
1107	30-31	0.3333877480540579
1107	32-33	0.3364672179652146
1107	34-35	0.33282260366422634
1107	36-37	0.2951964583722031
1107	38-39	0.3028888210572376
1107	40-41	0.3241553450782888
1107	42-43	0.3032664456819987
1107	44-45	0.22461638277157903
1107	46-47	0.2574505380796026
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2311	84-85	-0.1870132764271304
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2313	78-79	-0.3170788735898
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2314	82-83	-0.3465418812586343
2314	84-85	-0.36516510521300916
2314	86-87	-0.3427814416032824
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2314	90-91	-0.364176098799355
2314	92-93	-0.36978419327835965
2314	94-95	-0.3535940115650291
2314	96-97	-0.38662030855972773
2314	98-99	-0.4770629992232607
2314	100	-0.4697277171376797
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2315	4	-0.24450105363940366
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2315	9	-0.318365444351862
2315	10-11	-0.3582180982411032
2315	12-13	-0.39330842560473656
2315	14-15	-0.5476879303785864
2315	16-17	-0.6098309931954446
2315	18-19	-0.5218129564497289
2315	20-21	-0.4986954219221005
2315	22-23	-0.48065143256376786
2315	24-25	-0.508998645052877
2315	26-27	-0.4797236542493195
2315	28-29	-0.4794922995009472
2315	30-31	-0.5760053169382147
2315	32-33	-0.5493699844046276
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2315	70-71	-0.5461114864461081
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2315	82-83	-0.6818365465550897
2315	84-85	-0.6829026304581767
2315	86-87	-0.6937837742939195
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2315	90-91	-0.734309481244459
2315	92-93	-0.7930781818319339
2315	94-95	-0.8198340044786292
2315	96-97	-0.7218850019280119
2315	98-99	-0.7809427716153898
2315	100	-0.7859092823143321
2316	1	-0.5138451671031845
2316	2	-0.4004465269871531
2316	3	-0.4175594239336107
2316	4	-0.31315235934354035
2316	5	-0.3837088890655025
2316	6	-0.3505456941717213
2316	7	-0.370618698853427
2316	8	-0.38571307679693945
2316	9	-0.5213889138856942
2316	10-11	-0.5880168663893883
2316	12-13	-0.5267008658183485
2316	14-15	-0.722507454198329
2316	16-17	-0.7998901282999853
2316	18-19	-0.7529341860723804
2316	20-21	-0.7721910123387872
2316	22-23	-0.6837538053522039
2316	24-25	-0.6362349970247934
2316	26-27	-0.6544169761826666
2316	28-29	-0.6693474814086855
2316	30-31	-0.7785436062833639
2316	32-33	-0.6718179849544086
2316	34-35	-0.6678807653973422
2316	36-37	-0.7012285039372443
2316	38-39	-0.715047490156806
2316	40-41	-0.7197385175514341
2316	42-43	-0.7004260959740236
2316	44-45	-0.7672414011344415
2316	46-47	-0.8786021816798879
2316	48-49	-0.7614662575527689
2316	50-51	-0.85558070082444
2316	52-53	-0.8204602029124146
2316	54-55	-0.8875486918078082
2316	56-57	-0.8643790760531402
2316	58-59	-0.8311131711144597
2316	60-61	-0.7460636342413309
2316	62-63	-0.7236097159651393
2316	64-65	-0.8265084250020038
2316	66-67	-0.7784927192003934
2316	68-69	-0.7301314661662772
2316	70-71	-0.76680657745068
2316	72-73	-0.7323069779068874
2316	74-75	-0.6882208834133294
2316	76-77	-0.7803290364135513
2316	78-79	-0.8350726097460885
2316	80-81	-0.8610904524725917
2316	82-83	-0.8174128169772388
2316	84-85	-0.830496076269835
2316	86-87	-0.8515359009108998
2316	88-89	-0.8496740379710239
2316	90-91	-0.8929554673293651
2316	92-93	-0.9010373679567785
2316	94-95	-0.9017000644809414
2316	96-97	-0.9423981694378263
2316	98-99	-0.9841291378733921
2316	100	-1.0464929320713132
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	49.0
3	43.0
4	82.0
5	144.0
6	353.0
7	890.0
8	2012.0
9	3672.0
10	6190.0
11	11361.0
12	18945.0
13	26835.0
14	31479.0
15	41451.0
16	56815.0
17	78396.0
18	79025.0
19	49064.0
20	32937.0
21	27680.0
22	28864.0
23	31931.0
24	35689.0
25	39875.0
26	48160.0
27	59744.0
28	74552.0
29	93450.0
30	117016.0
31	146787.0
32	187105.0
33	243536.0
34	345595.0
35	559027.0
36	1078474.0
37	2161532.0
38	2025688.0
39	398648.0
40	449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.367606490785036	18.501119598405854	13.633890400310921	23.497383510498192
2	14.610305713771405	22.994202245009674	36.14373419164109	26.25175784957784
3	19.987532841443535	27.95163970771576	29.477042618029596	22.583784832811105
4	13.875654200632129	19.067775535484486	33.91724107949536	33.139329184388025
5	13.255467981081948	37.79241104457579	33.14921204463167	15.802908929710588
6	31.054534603787413	33.61524986968206	18.261199514462067	17.069016012068456
7	24.64901956089148	33.05862496001434	22.44819670057696	19.84415877851722
8	28.17028701873693	31.17939423187322	21.523906357734866	19.126412391654984
9	28.50876368952342	14.466930556655608	19.775159343995767	37.24914640982521
10-11	24.897926730244688	26.513004944104452	25.49074649683875	23.09832182881211
12-13	27.625106825596095	24.4263038002989	25.99257778920334	21.95601158490166
14-15	26.325262305488522	23.509609277227554	25.12202814744329	25.04310026984063
16-17	22.94777158368185	26.697396668450295	27.983998295574725	22.37083345229313
18-19	24.678140785124906	25.43606009422186	29.30936711223429	20.576432008418937
20-21	24.343419061655673	25.30880517803422	28.438117760786074	21.909657999524025
22-23	22.881291616998173	26.387026554934405	25.87838475912966	24.85329706893776
24-25	23.03722170173139	27.26881114893206	25.401724334724605	24.292242814611946
26-27	24.237367974587855	25.27308869838404	26.010197325280455	24.47934600174765
28-29	23.462034113189457	24.551664802927036	28.74630700789844	23.23999407598507
30-31	25.22152558781176	25.0502510665285	27.388960293353133	22.339263052306606
32-33	21.817173756676	26.15980281066637	28.191554124683755	23.831469307973872
34-35	21.407330781087445	26.99570425973572	28.53499686126805	23.06196809790879
36-37	24.11859869786249	26.342827768749146	26.75253640264927	22.786037130739096
38-39	24.786562675587486	25.02920074955805	26.46461338256706	23.719623192287404
40-41	23.157072766756702	26.428837505216606	26.69279764159345	23.721292086433238
42-43	25.57707564413366	25.326938154167188	26.417009324757718	22.67897687694143
44-45	25.391952887186214	24.92638622845633	26.703701852651623	22.977959031705833
46-47	23.604323295863793	25.67364459224838	28.721958555121617	22.000073556766207
48-49	24.731491239838192	26.258903910675237	27.501630893434893	21.507973956051682
50-51	22.79412753417847	27.091218175813314	26.466515464345424	23.64813882566279
52-53	22.30596165324888	27.527186131816904	24.408556838097876	25.758295376836344
54-55	23.410424625901264	27.16248674761534	24.035645498978955	25.39144312750444
56-57	24.36885264778902	25.102019446751733	24.735954995800817	25.79317290965843
58-59	23.69729693478272	24.96793675579971	26.855981727313278	24.47878458210429
60-61	24.418203319681474	25.742117181297214	25.839273677197728	24.000405821823584
62-63	21.60461522184932	26.352560374673313	26.795978401009325	25.246846002468043
64-65	21.16437586949889	26.637144716237497	26.85137217263384	25.347107241629768
66-67	23.76659722911249	26.20270968553187	25.40962590169764	24.621067183657992
68-69	24.327832540383465	25.0494386150704	25.251220861179906	25.371507983366225
70-71	22.659867567250785	26.0878038201986	25.62324481049648	25.629083802054133
72-73	24.387726100483757	25.340028345391353	25.604215300948596	24.6680302531763
74-75	24.69975152676234	24.651612791910924	25.827677697170643	24.820957984156095
76-77	22.930964953708855	25.666062255125528	27.504372221235744	23.898600569929876
78-79	23.44052467236221	25.760304853818607	26.9274277566101	23.87174271720908
80-81	21.943668267320927	25.95439087031508	26.747890507144	25.354050355219993
82-83	21.76683719045708	26.55336563295713	24.82396103316799	26.855836143417793
84-85	22.36318732974243	26.43210990202791	24.355661368072674	26.84904140015698
86-87	22.929913701608733	24.686979991008766	25.194084501127218	27.18902180625528
88-89	22.839705717570528	24.161465456504622	26.5489229956883	26.449905830236553
90-91	23.506060757324665	24.936920515275045	25.746855266267417	25.810163461132873
92-93	21.484918500042458	25.538368438861543	26.3382546790094	26.638458382086604
94-95	20.825816527431538	25.852194268770894	26.882682814184065	26.439306389613503
96-97	22.779571054751603	25.22364523950199	25.650069469229784	26.346714236516615
98-99	23.21146994337233	24.52210922884321	25.45983966442133	26.80658116336313
100	22.32619235704284	25.54753372364192	26.24739499901209	25.87887892030315
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1711.0
1	1073.0
2	515.5
3	590.0
4	540.5
5	627.0
6	988.0
7	2002.0
8	3064.5
9	3129.5
10	2455.0
11	2046.5
12	2244.0
13	2566.0
14	3038.5
15	3490.0
16	4031.0
17	4814.0
18	5611.0
19	6608.5
20	7884.5
21	9375.0
22	11355.0
23	13669.0
24	16338.0
25	20470.0
26	26399.5
27	32776.0
28	39296.5
29	48274.5
30	58042.5
31	67551.5
32	78381.5
33	89691.0
34	101136.0
35	109965.0
36	118424.5
37	128570.5
38	135173.0
39	141301.0
40	145969.5
41	147482.0
42	151945.5
43	164623.0
44	182792.0
45	201643.0
46	228650.5
47	270056.0
48	375844.0
49	550011.5
50	854419.0
51	828882.5
52	469389.0
53	321776.5
54	275199.5
55	240815.0
56	218653.5
57	196277.0
58	182829.5
59	167582.0
60	140526.0
61	112847.5
62	89410.0
63	70114.5
64	51293.5
65	39408.0
66	32108.5
67	24420.5
68	18231.5
69	14943.0
70	12656.0
71	10572.5
72	9370.5
73	9715.0
74	7453.0
75	5343.5
76	4281.0
77	3157.5
78	2317.0
79	1439.5
80	1104.5
81	974.5
82	804.5
83	672.0
84	483.5
85	273.5
86	180.5
87	92.5
88	44.0
89	32.0
90	23.5
91	17.0
92	12.0
93	7.0
94	4.5
95	2.0
96	1.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.062012305451741224
3	0.02646267688089155
4	0.007269561351966496
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	9.209748334417013E-5
12-13	1.7805513446539562E-4
14-15	4.236484233831826E-4
16-17	4.604874167208507E-4
18-19	0.0
20-21	0.0027383651714333254
22-23	0.004764509805005069
24-25	6.938010411927483E-4
26-27	4.54347584497906E-4
28-29	0.005145179402827638
30-31	0.0022778777547124746
32-33	0.0012586656057036586
34-35	0.0015840767135197262
36-37	0.0039172129582387034
38-39	0.004254903730500661
40-41	9.88512987894093E-4
42-43	0.00274450500365627
44-45	3.4383060448490185E-4
46-47	0.0021428014458076917
48-49	3.069916111472338E-5
50-51	0.0018603691635522368
52-53	0.006882751921920982
54-55	0.014029516629428584
56-57	0.025572401208564573
58-59	0.027181037250976082
60-61	0.025062795134060168
62-63	0.008159837024293475
64-65	0.006931870579704539
66-67	2.08754295580119E-4
68-69	0.0013876020823854966
70-71	1.9033479891128496E-4
72-73	8.5343667898931E-4
74-75	0.005089920912821137
76-77	0.0010683308067923737
78-79	0.0012402461090348245
80-81	0.0
82-83	0.001307784263487216
84-85	1.2279664445889352E-5
86-87	6.876612089698037E-4
88-89	9.209748334417013E-5
90-91	6.07843390071523E-4
92-93	9.148350012187568E-4
94-95	0.0015472377201820584
96-97	1.4121614112772756E-4
98-99	0.0
100	9.08695168995812E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8143545.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.830371180536392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.44907844626867	24.33402543354676
2	12.862167869885221	8.188151549696315
3	3.963448565122543	3.7847411694844473
4	1.8290983273691261	2.3288351474343023
5	1.0304437158835136	1.6399702978611712
6	0.6365205765679557	1.215641172972424
7	0.4640989908335618	1.0340710199921241
8	0.35116001348600717	0.8942042858417419
9	0.2729161088224276	0.781831894046898
>10	1.7300260342687328	10.65648609007106
>50	0.2013638030433445	4.491266909107694
>100	0.17473788583733618	11.355913296031789
>500	0.022191957777504896	4.839813567082541
>1k	0.01049353751602617	5.90248323290516
>5k	6.607042139580835E-4	1.4728621066415815
>10k+	0.0015934631042518485	17.07970282728423
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	171809	2.1097568687838035	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	158606	1.9476284591047266	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	78992	0.9699952539096915	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	64572	0.7929224925999672	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	59421	0.7296699410391911	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	57777	0.709482172690149	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	57620	0.7075542653721445	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	51978	0.6382723985684368	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	46699	0.5734480499585869	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	41964	0.5153038388073007	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	36101	0.4433081661610515	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	35536	0.436370155749124	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	32862	0.40353433302081587	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27693	0.3400607475000138	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	26910	0.33044577023888244	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	26507	0.32549706546718904	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	23538	0.28903874172734356	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	22950	0.2818182990331606	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	22669	0.27836771332386573	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	22300	0.27383651714333257	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	20399	0.25049287503169687	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19933	0.24477055139991247	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	19032	0.23370657373416615	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	16975	0.20844730396897176	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	16851	0.20692462557768146	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	16706	0.20514407423302752	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	16022	0.1967447837520392	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15987	0.19631499549643305	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	15985	0.19629043616754127	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	15780	0.19377310495613398	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	15642	0.19207851126260125	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	15640	0.19205395193370944	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	14197	0.17433439613829113	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	13789	0.16932429304436827	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	12886	0.1582357560497302	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	12273	0.15070832174440002	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	12055	0.14803135489519614	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	11352	0.1393987507897359	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	11170	0.13716385186058408	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	11049	0.13567801246263145	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG	10413	0.12786814587504583	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	9969	0.12241597486107095	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	9953	0.12221950022993672	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	8834	0.10847855571498653	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	8705	0.10689447900146681	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.003696178998212695	0.0	0.0	3.6838993337668056E-5	0.0
2	0.005832840611797442	3.6838993337668056E-5	0.0	3.6838993337668056E-5	0.0
3	0.00625034920295768	3.6838993337668056E-5	0.0	3.6838993337668056E-5	0.0
4	0.007551993634221951	6.139832222944676E-5	0.0	6.139832222944676E-5	0.0
5	0.031902568230420536	6.139832222944676E-5	0.0	3.683899333766805E-4	0.0
6	0.06637158633003194	6.139832222944676E-5	0.0	7.245002023074718E-4	0.0
7	0.07413233425983401	6.139832222944676E-5	0.0	7.981781889828079E-4	0.0
8	0.08472968467663652	7.367798667533611E-5	0.0	9.08695168995812E-4	0.0
9	0.09209748334417013	1.2279664445889353E-4	0.0	0.0010314918134547056	0.0
10-11	0.15961721829989273	1.2279664445889353E-4	0.0	0.0018603691635522368	0.0
12-13	0.27640910684474634	1.2279664445889353E-4	0.0	0.003935632454907537	0.0
14-15	0.4168516291123829	1.5963563779656156E-4	0.0	0.0063792856796395184	6.139832222944676E-6
16-17	0.4815961599033345	1.7191530224245093E-4	0.0	0.007502874976438393	1.2279664445889352E-5
18-19	0.5125531939714216	1.8419496668834026E-4	0.0	0.0082457946754147	1.2279664445889352E-5
20-21	0.6241630641201099	2.08754295580119E-4	0.0	0.010548231759018954	1.2279664445889352E-5
22-23	0.6552735939937706	2.2717379224895302E-4	0.0	0.01296118582263621	1.8419496668834028E-5
24-25	0.7956178789458399	2.517331211407317E-4	0.0	0.023902366843923623	2.4559328891778703E-5
26-27	1.092245453300743	2.701526178095657E-4	0.0	0.06296397944629764	2.4559328891778703E-5
28-29	1.1611405106744055	2.8243228225545506E-4	0.0	0.09393329317883059	2.4559328891778703E-5
30-31	1.1815861519768112	2.9471194670134445E-4	0.0	0.16467644005159915	2.4559328891778703E-5
32-33	1.202105471265892	3.069916111472338E-4	0.0	0.2798105738962577	2.4559328891778703E-5
34-35	1.2176638061188338	3.806695978225699E-4	0.0	0.4278357889592309	2.4559328891778703E-5
36-37	1.240639058297093	4.54347584497906E-4	0.0	0.6485136387163084	3.069916111472338E-5
38-39	1.2640011199053975	4.54347584497906E-4	0.0	0.9719354408921421	3.6838993337668056E-5
40-41	1.2836363033543745	5.034662422814634E-4	0.0	1.4265838771689725	3.6838993337668056E-5
42-43	1.3247117809258744	5.280255711732421E-4	0.0	1.760019745700429	3.6838993337668056E-5
44-45	1.3731304978360162	6.139832222944676E-4	0.0	2.1061220881078206	3.6838993337668056E-5
46-47	1.3928700584327833	6.63101880078025E-4	0.0	2.5079863867639953	5.525849000650208E-5
48-49	1.40697939288111	6.63101880078025E-4	0.0	2.873251145539197	7.367798667533611E-5
50-51	1.4311150733495057	7.122205378615824E-4	0.0	3.2666915943854917	7.367798667533611E-5
52-53	1.439281050206022	7.122205378615824E-4	0.0	3.726687824528507	8.595765112122546E-5
54-55	1.4630729000699327	7.306400345304164E-4	0.0	4.13822235893582	9.823731556711481E-5
56-57	1.5092321587220308	7.490595311992504E-4	0.0	4.576188871062909	9.823731556711481E-5
58-59	1.5239984552182126	7.551993634221951E-4	0.0	5.034343151539041	9.823731556711481E-5
60-61	1.5340125215738354	7.920383567598632E-4	0.0	5.4504702804491165	9.823731556711481E-5
62-63	1.5423074349070338	8.104578534286972E-4	0.0	5.880356773370811	9.823731556711481E-5
64-65	1.5487481189089025	8.841358401040333E-4	0.0	6.352178320375217	9.823731556711481E-5
66-67	1.557006193248763	9.025553367728674E-4	0.0	6.827542550572263	9.823731556711481E-5
68-69	1.5668115053088059	9.393943301105353E-4	0.0	7.386261143028006	9.823731556711481E-5
70-71	1.5783605297201648	9.4553416233348E-4	0.0	8.077452755526004	9.823731556711481E-5
72-73	1.6082553728136824	9.578138267793694E-4	0.0	8.644368023999377	9.823731556711481E-5
74-75	1.638831737283947	0.0010130723167858717	0.0	9.18474079777296	9.823731556711481E-5
76-77	1.6488458036395697	0.0010499113101235396	0.0	9.777989806650544	9.823731556711481E-5
78-79	1.6559066106959561	0.0010560511423464843	0.0	10.35208253899254	9.823731556711481E-5
80-81	1.6702001401109714	0.0010560511423464843	0.0	10.983220452517914	9.823731556711481E-5
82-83	1.6752409423660088	0.0010683308067923735	0.0	11.725434070788582	9.823731556711481E-5
84-85	1.6945077358816092	0.0010928901356841523	0.0	12.414120631739618	9.823731556711481E-5
86-87	1.7282276944500214	0.001117449464575931	0.0	13.097711131945609	9.823731556711481E-5
88	1.736369111977646	0.0012156867801430458	0.0	13.656472703226912	9.823731556711481E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATC	2360	0.0	30.475327	3
CGATCTC	2370	0.0	25.380169	5
TCTCGCG	1205	0.0	24.959076	8
GATCTAC	3270	0.0	24.739737	1
CCGATCT	3225	0.0	22.73288	4
CTCGCGC	1450	0.0	21.065943	9
AGTACTT	31865	0.0	20.550793	12-13
TACACTC	4370	0.0	20.109121	5
TCTACAC	4915	0.0	18.745691	3
GAGTACT	31405	0.0	18.682083	12-13
ATCTACA	4445	0.0	18.517427	2
TACTTTT	36690	0.0	17.989988	14-15
CTACACT	6125	0.0	17.877607	4
GTATAAG	2545	0.0	17.741806	1
GTACTAG	1725	0.0	17.177732	1
GTACTTT	34500	0.0	17.034191	14-15
AGTACTC	2935	0.0	16.971907	5
GTGATCG	1555	0.0	16.923618	8
CATGGGG	19750	0.0	15.919103	4
ACTTTTT	37445	0.0	15.864832	16-17
>>END_MODULE