FastQCFastQC Report
Fri 27 May 2016
ERR765422_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765422_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7469542
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA715740.9582113602145889No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT631820.8458617676960649No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG297040.3976682907733834No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC214640.28735362891058114No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT195020.2610869582097537No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC189390.25354968216257434No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG184060.2464140371658664No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG167930.22481967435218922No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC123990.16599411315981624No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG113550.15201735260341262No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT106480.14255224751397072No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC81520.10913654411475295No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA77100.10321917997114147No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG76990.10307191525263532No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC75420.10097004608850182No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19150.022.5828111
TCTAACG8150.021.1099662
GTACAAG35350.021.0472011
GTACATA19750.020.2486081
CTAACGC8550.020.1223683
GTATAAG25850.019.4279541
TAGTACT23650.016.7121074
ACGCCTA10450.016.4637556
GTATAAT21000.016.164691
TAGGCAT27950.016.1373925
CATGGGG174400.016.0240174
GTATTAG25600.015.803131
TACTAGG26800.015.615332
TATATAC23300.015.3666163
GTATTAT17600.015.3242471
ACGTTAA5850.015.1022348
GTATTAA21450.014.9584171
CTAGTAC23200.014.8315723
TATACAG33100.014.7504325
ATAAGGT20400.014.5879163
Produced by FastQC (version 0.11.5)