##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765422_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7469542 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.49230849762944 33.0 33.0 33.0 27.0 33.0 2 31.38837936783808 33.0 33.0 33.0 27.0 33.0 3 31.442851248443343 33.0 33.0 33.0 27.0 33.0 4 35.067031285184555 37.0 37.0 37.0 33.0 37.0 5 35.206442510129804 37.0 37.0 37.0 33.0 37.0 6 35.130869737394875 37.0 37.0 37.0 33.0 37.0 7 35.02180508523816 37.0 37.0 37.0 33.0 37.0 8 34.94685551001655 37.0 37.0 37.0 33.0 37.0 9 34.90318415774354 37.0 37.0 37.0 33.0 37.0 10-11 34.894361997027396 37.0 37.0 37.0 33.0 37.0 12-13 34.84335023486045 37.0 37.0 37.0 33.0 37.0 14-15 36.32633547009978 37.0 37.0 40.0 33.0 40.0 16-17 36.36653472194145 37.0 37.0 40.0 33.0 40.0 18-19 36.379818535058774 37.0 37.0 40.0 33.0 40.0 20-21 36.393823075096165 37.0 37.0 40.0 33.0 40.0 22-23 36.45592541015232 37.0 37.0 40.0 33.0 40.0 24-25 36.42738088894875 37.0 37.0 40.0 33.0 40.0 26-27 36.43053108209312 37.0 37.0 40.0 33.0 40.0 28-29 36.35290651019835 37.0 37.0 40.0 33.0 40.0 30-31 36.30862387546653 37.0 37.0 40.0 33.0 40.0 32-33 36.15267709854233 37.0 37.0 40.0 27.0 40.0 34-35 36.11412326217591 37.0 37.0 40.0 27.0 40.0 36-37 36.01582894908416 37.0 37.0 40.0 27.0 40.0 38-39 35.81046635791057 37.0 37.0 40.0 27.0 40.0 40-41 35.71799167874014 37.0 37.0 40.0 27.0 40.0 42-43 35.579563178036885 37.0 35.0 40.0 27.0 40.0 44-45 35.44016614405542 37.0 33.0 40.0 27.0 40.0 46-47 35.30036185618877 37.0 33.0 40.0 27.0 40.0 48-49 35.13362921046565 37.0 33.0 40.0 27.0 40.0 50-51 34.97707048169754 37.0 33.0 40.0 27.0 40.0 52-53 34.8148799484627 37.0 33.0 40.0 27.0 40.0 54-55 34.680979904792025 37.0 33.0 40.0 27.0 40.0 56-57 34.477118610485086 37.0 33.0 40.0 27.0 40.0 58-59 34.24496977190837 37.0 33.0 37.0 27.0 40.0 60-61 33.994015630409464 37.0 33.0 37.0 27.0 40.0 62-63 33.77436722358613 37.0 33.0 37.0 27.0 40.0 64-65 33.48355816193282 37.0 33.0 37.0 24.5 40.0 66-67 33.25646150727849 37.0 33.0 37.0 24.5 40.0 68-69 32.948893921474706 37.0 33.0 37.0 22.0 40.0 70-71 32.742772850062295 37.0 33.0 37.0 22.0 37.0 72-73 32.433965563082715 33.0 33.0 37.0 22.0 37.0 74-75 32.15602328763933 33.0 33.0 37.0 22.0 37.0 76-77 31.940239107029587 33.0 33.0 37.0 22.0 37.0 78-79 31.692688989498954 33.0 33.0 37.0 22.0 37.0 80-81 31.442397137602278 33.0 30.0 37.0 22.0 37.0 82-83 31.28451456595331 33.0 27.0 37.0 22.0 37.0 84-85 31.088178231008005 33.0 27.0 37.0 22.0 37.0 86-87 30.8607547156171 33.0 27.0 37.0 18.5 37.0 88-89 30.66735878853081 33.0 27.0 37.0 15.0 37.0 90-91 30.46515803780205 33.0 27.0 37.0 15.0 37.0 92-93 30.28441703654655 33.0 27.0 37.0 15.0 37.0 94-95 30.06748546296413 33.0 27.0 37.0 15.0 37.0 96-97 29.994568475550444 33.0 27.0 37.0 15.0 37.0 98-99 28.826031435394565 33.0 24.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24028852490691222 1101 2 0.23360351103123733 1101 3 0.27576714654172463 1101 4 0.33992773922405206 1101 5 0.3122423194609638 1101 6 0.3012029985660334 1101 7 0.2556358774897802 1101 8 0.34470196813093423 1101 9 0.2511961185489682 1101 10-11 0.25883413397768607 1101 12-13 0.2700790404507032 1101 14-15 0.3628623019063326 1101 16-17 0.3670969961466355 1101 18-19 0.281771074997053 1101 20-21 0.2647230674815617 1101 22-23 0.23732304796309478 1101 24-25 0.2956787427297485 1101 26-27 0.3848515139042732 1101 28-29 0.25840912978880226 1101 30-31 0.2590392302527391 1101 32-33 0.38043374872442826 1101 34-35 0.3888306582015417 1101 36-37 0.3070579516840013 1101 38-39 0.2700531909553945 1101 40-41 0.26686734208720253 1101 42-43 0.3132258993015071 1101 44-45 0.27254996879960913 1101 46-47 0.24711202478571437 1101 48-49 0.24196538967690628 1101 50-51 0.22846082189307282 1101 52-53 0.17261507687590694 1101 54-55 0.3455291699060794 1101 56-57 0.3151719850453034 1101 58-59 0.26858005051877853 1101 60-61 0.3472820334553077 1101 62-63 0.2748323856959871 1101 64-65 0.12098259100801556 1101 66-67 0.20622546369491346 1101 68-69 0.22413261067592316 1101 70-71 0.2137879363703945 1101 72-73 0.2116365489423515 1101 74-75 0.20012836851028482 1101 76-77 0.1514254838670155 1101 78-79 0.20893551699034063 1101 80-81 0.16948169406157731 1101 82-83 0.21319410181735066 1101 84-85 0.21354608727288493 1101 86-87 0.2235744356015239 1101 88-89 0.2639897938342486 1101 90-91 0.17526326271444503 1101 92-93 0.2770387931788676 1101 94-95 0.2705184061905328 1101 96-97 0.1572663598477746 1101 98-99 0.16703040518411783 1102 1 0.21707025796577284 1102 2 0.3023756718603181 1102 3 0.2874550842966208 1102 4 0.3711166784075459 1102 5 0.2881157406144226 1102 6 0.2917131541755822 1102 7 0.21693957683777398 1102 8 0.2663712297305878 1102 9 0.30822235575422496 1102 10-11 0.26702388173035274 1102 12-13 0.3026642499088439 1102 14-15 0.35796641318926703 1102 16-17 0.3485847954268593 1102 18-19 0.3024667565782906 1102 20-21 0.36668007455286045 1102 22-23 0.30059323730463916 1102 24-25 0.4090581337228798 1102 26-27 0.39442984956419025 1102 28-29 0.2598581443171213 1102 30-31 0.31282668939614666 1102 32-33 0.45306430655713115 1102 34-35 0.33308089435431754 1102 36-37 0.3955416788293604 1102 38-39 0.39901732054156724 1102 40-41 0.33698957895571624 1102 42-43 0.2958854596716307 1102 44-45 0.33966772380698274 1102 46-47 0.40780986943352104 1102 48-49 0.35378926932365573 1102 50-51 0.3767807961763481 1102 52-53 0.2895974141529578 1102 54-55 0.3821708173961724 1102 56-57 0.3966319637523341 1102 58-59 0.3314128330690451 1102 60-61 0.4186660071406365 1102 62-63 0.3823753941494701 1102 64-65 0.2335772841886694 1102 66-67 0.2945682394046756 1102 68-69 0.29904946105254027 1102 70-71 0.29526499634119574 1102 72-73 0.28956016588415423 1102 74-75 0.3126016362724826 1102 76-77 0.2633399794379727 1102 78-79 0.2913490095322011 1102 80-81 0.31871050041054616 1102 82-83 0.28488266936632556 1102 84-85 0.2045587497349466 1102 86-87 0.26713606355279396 1102 88-89 0.3026909319769757 1102 90-91 0.25430444804611696 1102 92-93 0.36558051419561366 1102 94-95 0.43176425571152066 1102 96-97 0.3098369319952887 1102 98-99 0.42586948103373246 1103 1 0.08455812506839422 1103 2 0.1542426188124857 1103 3 0.15951688291345434 1103 4 0.17069896420579767 1103 5 0.09543710228161473 1103 6 0.12352801007666159 1103 7 0.05981298728549689 1103 8 0.07139357229304721 1103 9 0.1888145943186359 1103 10-11 0.10253543032699497 1103 12-13 0.08658069334227037 1103 14-15 0.10168750036300622 1103 16-17 0.04413053949939183 1103 18-19 0.1059587490375975 1103 20-21 0.19135623296106985 1103 22-23 0.14037032563388152 1103 24-25 0.16509335133513758 1103 26-27 0.22745617023829823 1103 28-29 0.12206593545128896 1103 30-31 0.2082212850353855 1103 32-33 0.2378029356996052 1103 34-35 0.1872204062183087 1103 36-37 0.20141081683133422 1103 38-39 0.21300347373692574 1103 40-41 0.21944662364801815 1103 42-43 0.2197277299779543 1103 44-45 0.13224562251426164 1103 46-47 0.09342755066111863 1103 48-49 0.16561481218834473 1103 50-51 0.1754469534646219 1103 52-53 0.17749685140864102 1103 54-55 0.24502627221643536 1103 56-57 0.16937653713323897 1103 58-59 0.2722084936143361 1103 60-61 0.25989365794903563 1103 62-63 0.1600169412393342 1103 64-65 0.22002041890446122 1103 66-67 0.19605092204700725 1103 68-69 0.24504300395216205 1103 70-71 0.13284216350267997 1103 72-73 0.2514629577997738 1103 74-75 0.2394314756038156 1103 76-77 0.1784192671463387 1103 78-79 0.16303887405117123 1103 80-81 0.12494030296181435 1103 82-83 0.1100822729589801 1103 84-85 0.24345049218965187 1103 86-87 0.3018021191421596 1103 88-89 0.31789616956401545 1103 90-91 0.2806513618743942 1103 92-93 0.2964052291189283 1103 94-95 0.2703379941226025 1103 96-97 0.11290489696594364 1103 98-99 0.13531055509892198 1104 1 0.1160411441775544 1104 2 0.061102200878600854 1104 3 0.08993331850027175 1104 4 0.1684012837973654 1104 5 0.11357857551912787 1104 6 0.12040944536003906 1104 7 0.00802047791200522 1104 8 0.01618466239028038 1104 9 0.05814775241928061 1104 10-11 0.0688841504628499 1104 12-13 0.04890343309398304 1104 14-15 0.08597658262372221 1104 16-17 0.10098426462054988 1104 18-19 0.03329769596510346 1104 20-21 0.08380635456294527 1104 22-23 0.04856580533662225 1104 24-25 0.06842746058886462 1104 26-27 0.09975011445126825 1104 28-29 0.07044150416244577 1104 30-31 0.11118365773940297 1104 32-33 0.0948358018052673 1104 34-35 0.12229656258086408 1104 36-37 0.16341625003522608 1104 38-39 0.09397902290423588 1104 40-41 0.09919616119938723 1104 42-43 0.09019500238457567 1104 44-45 0.02335387907066888 1104 46-47 -0.003748522092465123 1104 48-49 0.035584352379366635 1104 50-51 0.0774762875954309 1104 52-53 -0.01698198163791176 1104 54-55 0.18221279996465256 1104 56-57 0.1005315830495519 1104 58-59 0.13009465594249292 1104 60-61 0.15776897185813255 1104 62-63 0.09619715472209123 1104 64-65 0.055757529087117064 1104 66-67 0.07284533426914663 1104 68-69 0.0043664807771506275 1104 70-71 -0.04724773334943677 1104 72-73 0.015390781934783604 1104 74-75 -0.11264733259189796 1104 76-77 -0.14104511223059646 1104 78-79 -0.08025168868515564 1104 80-81 -0.05546749010303742 1104 82-83 0.0488450143104977 1104 84-85 -0.0030597352768850783 1104 86-87 0.006506323452150298 1104 88-89 0.15223350567638505 1104 90-91 0.09929425941603398 1104 92-93 0.08644879301179031 1104 94-95 0.04545901439258415 1104 96-97 -0.2148812895644241 1104 98-99 -0.08458626722284635 1105 1 0.10362833609605104 1105 2 0.0923898286192042 1105 3 0.09537841881776998 1105 4 0.0640091631135391 1105 5 0.054535617486195065 1105 6 0.05487052411373128 1105 7 -0.0034692167070033975 1105 8 -0.033031400626931884 1105 9 0.005992870273026085 1105 10-11 0.012611840739353397 1105 12-13 0.011670461758512829 1105 14-15 0.0727775776100188 1105 16-17 0.06378989056649687 1105 18-19 0.0022031710637975266 1105 20-21 0.09278667865855539 1105 22-23 0.06645005846626617 1105 24-25 0.0561235321587219 1105 26-27 0.10483611451132901 1105 28-29 0.07138247444663648 1105 30-31 0.06403252016982464 1105 32-33 0.09363497808921295 1105 34-35 0.0672736249286956 1105 36-37 0.08931585796680253 1105 38-39 0.009553076020942797 1105 40-41 -0.016400185582867266 1105 42-43 0.03883217371126335 1105 44-45 0.02204707874663825 1105 46-47 -0.020366896942846324 1105 48-49 -0.02583702873855742 1105 50-51 0.030068754630967476 1105 52-53 -0.034657673610318795 1105 54-55 0.08699645426553104 1105 56-57 0.05449702272164103 1105 58-59 0.028547028764030813 1105 60-61 0.11236421670255936 1105 62-63 0.1317236527279917 1105 64-65 0.013533108417561834 1105 66-67 0.060247100112817975 1105 68-69 0.0136979605716121 1105 70-71 -0.042550550930805286 1105 72-73 -0.03684770630863454 1105 74-75 0.0015152755774181514 1105 76-77 -0.02991657250962021 1105 78-79 0.07208337465246117 1105 80-81 -0.034214491866610075 1105 82-83 0.04478472778647813 1105 84-85 0.05807315396377177 1105 86-87 0.010332499708475495 1105 88-89 0.0687522199787054 1105 90-91 0.1175239126117198 1105 92-93 0.10854955365805807 1105 94-95 0.05409848704093889 1105 96-97 -0.0459527057746314 1105 98-99 0.0948049198686185 1106 1 0.00520843269466198 1106 2 0.11046845850586351 1106 3 0.0442447150995946 1106 4 0.027732372327371024 1106 5 0.0038194082152571696 1106 6 -0.034176939646762605 1106 7 -0.008742767502425863 1106 8 -0.004962997148901138 1106 9 0.03190610133726324 1106 10-11 -0.06081470118666488 1106 12-13 0.01946271463408067 1106 14-15 -0.011287881924658905 1106 16-17 -0.0387112628161006 1106 18-19 0.042652268945786886 1106 20-21 0.022005406586487197 1106 22-23 0.07436075284016397 1106 24-25 0.07788885749526742 1106 26-27 0.03426307120425065 1106 28-29 0.008056938029803007 1106 30-31 0.012170085277709575 1106 32-33 0.07464862482202506 1106 34-35 -0.025407895702954875 1106 36-37 -0.0999592654864685 1106 38-39 -0.03138386137630533 1106 40-41 0.06152732112703774 1106 42-43 -0.05778214115692748 1106 44-45 -0.030703453236995415 1106 46-47 -0.06515056068711544 1106 48-49 -0.10470926850990026 1106 50-51 -0.09553277760213774 1106 52-53 -0.057818358967544725 1106 54-55 -0.028686986917222157 1106 56-57 -0.09766878560796499 1106 58-59 -0.031544246253481845 1106 60-61 0.031245664749995683 1106 62-63 0.004085062725074806 1106 64-65 -0.09520241606720958 1106 66-67 0.02467805419691871 1106 68-69 -0.02028205472667821 1106 70-71 -0.1339623400789307 1106 72-73 0.03166507632646187 1106 74-75 -0.06877219094818798 1106 76-77 -0.14453728996620185 1106 78-79 -0.04522479941138258 1106 80-81 -0.09003097028520202 1106 82-83 -0.10576936548197224 1106 84-85 -0.16677136480142352 1106 86-87 -0.16510562037556298 1106 88-89 -0.014404839925056478 1106 90-91 -0.09657833494401302 1106 92-93 -0.1218279480926796 1106 94-95 -0.23177114090112738 1106 96-97 -0.3302894844784561 1106 98-99 0.04350412219372046 1107 1 0.05043427691907354 1107 2 0.09633137545642256 1107 3 0.07761260935364689 1107 4 0.03111438737578709 1107 5 0.0696605503847394 1107 6 1.4222422524312606E-4 1107 7 -0.07328047555370887 1107 8 0.0036792387000659232 1107 9 -0.0915022897327944 1107 10-11 2.360518240465126E-4 1107 12-13 -0.040219814514479424 1107 14-15 -0.0629707192973541 1107 16-17 -0.05786699784953697 1107 18-19 -0.09953894651910389 1107 20-21 -0.10948425242028748 1107 22-23 -0.1238170600368278 1107 24-25 -0.021497146032345427 1107 26-27 0.02504520467562088 1107 28-29 -0.03715960785429928 1107 30-31 -0.07823198867248493 1107 32-33 -0.15433232574708455 1107 34-35 -0.10174698206174071 1107 36-37 -0.13980812137030085 1107 38-39 -0.11350330781167628 1107 40-41 -0.2051949991665012 1107 42-43 -0.21791541491010236 1107 44-45 -0.14843573108704078 1107 46-47 -0.2724956485678547 1107 48-49 -0.18007952089974566 1107 50-51 -0.12176057145476449 1107 52-53 -0.23148229954664856 1107 54-55 -0.1655686362259985 1107 56-57 -0.16307015904877886 1107 58-59 -0.1295571702828724 1107 60-61 -0.027870915495938675 1107 62-63 -0.24957845017462432 1107 64-65 -0.26433619566189037 1107 66-67 -0.12327451541896295 1107 68-69 -0.18172638340272584 1107 70-71 -0.19575045663778923 1107 72-73 -0.12695125542298769 1107 74-75 -0.21193139977076925 1107 76-77 -0.2178452169303533 1107 78-79 -0.054861917117605685 1107 80-81 -0.3060655157209773 1107 82-83 -0.19027743461266056 1107 84-85 -0.2691031577514984 1107 86-87 -0.3751008864069014 1107 88-89 -0.1932311032752949 1107 90-91 -0.0719833694680645 1107 92-93 -0.11419607167137968 1107 94-95 1.7679134265691232E-4 1107 96-97 -0.11449849878064455 1107 98-99 0.12212894854113188 1108 1 0.08642557663493022 1108 2 0.05688544873530432 1108 3 0.04396862053929951 1108 4 0.060501373680857284 1108 5 0.09115972221182744 1108 6 -0.08198410215671714 1108 7 0.05763306287663994 1108 8 -0.03834100731928913 1108 9 0.03469511619597654 1108 10-11 -0.02066464432254378 1108 12-13 -0.03850748797396619 1108 14-15 0.03500212657534263 1108 16-17 -0.011990342002349053 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-0.1826516331145953 2215 90-91 0.10935740249048465 2215 92-93 0.10885121326286296 2215 94-95 0.14279560991046836 2215 96-97 0.15756148735382425 2215 98-99 0.08796215803446117 2216 1 -0.0169387040476785 2216 2 -0.09608735676164315 2216 3 -0.02625446933972242 2216 4 -0.028544166710268826 2216 5 -0.059254962390447474 2216 6 -0.05548778020731504 2216 7 -0.018088133465290923 2216 8 -0.04190445801531695 2216 9 -0.003034770513103524 2216 10-11 -0.036831813302271144 2216 12-13 -0.016803589916534634 2216 14-15 -0.08580029124017585 2216 16-17 -0.08220414798196174 2216 18-19 -0.13258231136029508 2216 20-21 -0.13815753231042294 2216 22-23 -0.2627813332894391 2216 24-25 -0.18251097379560122 2216 26-27 -0.2746565707486184 2216 28-29 -0.1089409857552539 2216 30-31 -0.16634586746241098 2216 32-33 -0.1496139190876562 2216 34-35 -0.28404043230989373 2216 36-37 -0.26240394195408356 2216 38-39 -0.2034192183306942 2216 40-41 -0.20799806120034958 2216 42-43 -0.21685951814319537 2216 44-45 -0.227219283376499 2216 46-47 -0.13479213375974552 2216 48-49 -0.13047589555346661 2216 50-51 -0.14136784777766565 2216 52-53 -0.056814639637693176 2216 54-55 -0.1371396737365842 2216 56-57 0.006803025576409993 2216 58-59 -0.19362438575166863 2216 60-61 -0.22332986389857012 2216 62-63 -0.17592451211727678 2216 64-65 -0.0024531903168139024 2216 66-67 -0.1927763237115201 2216 68-69 -0.1863532710314928 2216 70-71 -0.209866353117242 2216 72-73 -0.278145252509745 2216 74-75 -0.1607252988740484 2216 76-77 -0.22567573672288077 2216 78-79 -0.1470261823997454 2216 80-81 -0.08132453348582658 2216 82-83 -0.1622707633956324 2216 84-85 -0.1871245678760367 2216 86-87 -0.21729758630324625 2216 88-89 -0.17036521593584553 2216 90-91 -0.02124426156487047 2216 92-93 0.040310559964318315 2216 94-95 0.022830673588135397 2216 96-97 -0.027832194805156973 2216 98-99 0.045484748629611715 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 241.0 8 1445.0 9 2625.0 10 4014.0 11 6811.0 12 12929.0 13 22485.0 14 33070.0 15 36951.0 16 38167.0 17 40473.0 18 41803.0 19 43604.0 20 46362.0 21 51188.0 22 56239.0 23 63256.0 24 72386.0 25 81794.0 26 93091.0 27 106812.0 28 128509.0 29 160280.0 30 203309.0 31 260859.0 32 346665.0 33 464096.0 34 630917.0 35 899145.0 36 1359617.0 37 1662091.0 38 497886.0 39 417.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.805708851721754 17.689627108423814 12.172919771284992 25.33174426856944 2 15.707549405304905 20.65123939325865 37.10539414598646 26.535817055449982 3 18.772196005344913 26.102616081250616 28.15050904802457 26.974678865379904 4 13.303774180532086 17.43679866851274 35.044919755454885 34.214507395500284 5 14.532832133482884 37.33278425906167 32.97093717392579 15.163446433529659 6 32.703959626975795 35.00256106733184 17.621535564027887 14.671943741664482 7 28.236322923145757 31.477257909521093 21.620616096676343 18.665803070656807 8 27.576576984238123 32.9970699676098 20.00086484552868 19.425488202623402 9 27.68138930070947 15.581611295578766 18.61008345625475 38.12691594745702 10-11 25.46289317336993 25.901119506390085 26.204518295767 22.431469024472985 12-13 26.73415585587443 23.626495439747174 25.679626675905965 23.959722028472427 14-15 25.091022983738494 24.295987625479583 23.126973514574253 27.48601587620767 16-17 24.459833012519375 26.61698669075025 24.9151353590354 24.008044937694976 18-19 25.34897721975457 26.089738835393124 25.481120529210493 23.080163415641817 20-21 25.84900090568129 25.697987084837504 24.67722469263399 23.77578731684721 22-23 24.942332475003155 25.77364850482131 23.782897264651567 25.501121755523965 24-25 24.604278606818635 26.421044429482272 23.621958796964655 25.352718166734434 26-27 25.21731091990343 25.618935410944676 23.75060738349399 25.413146285657906 28-29 24.810803533778348 25.512072443969082 24.810863783699038 24.866260238553537 30-31 25.493741346473353 25.76537941371696 24.27751503514694 24.46336420466275 32-33 24.165010216496004 26.13385366898553 24.283277244407703 25.417858870110766 34-35 24.266229620390124 26.342516276443906 24.820454065626826 24.57080003753914 36-37 25.22145266737907 25.921990933312912 24.147464463015268 24.709091936292747 38-39 25.53221326859752 25.497365064789477 24.06687508204155 24.90354658457146 40-41 24.698274673333383 26.217731957327505 24.373837110762615 24.710156258576497 42-43 25.610365401252178 25.7700873761738 24.12923710717471 24.49031011539931 44-45 25.311592062806525 25.788729750766514 24.030000768454077 24.869677417972884 46-47 24.43650494233783 25.88962616449576 25.426485318644705 24.247383574521706 48-49 25.294548456317813 25.95501870165398 24.793820598936225 23.956612243091982 50-51 24.031667537313535 26.36891257857577 24.817016893405246 24.782402990705453 52-53 23.901224465971275 26.326801562933845 23.972400181965643 25.799573789129237 54-55 24.355669999579625 26.20544204718308 23.993646464535576 25.445241488701715 56-57 24.850452678356984 25.231259158861413 24.013487038428863 25.904801124352737 58-59 24.564096433221742 25.289268070251165 24.87325862817292 25.273376868354173 60-61 24.964790344575345 25.685021919683965 24.432883569032747 24.917304166707947 62-63 23.790889722555946 25.985810107232815 24.62531169916442 25.59798847104682 64-65 23.629855752869453 25.90262562282935 24.880976638192813 25.586541986108386 66-67 24.94729259025123 25.59632871537704 24.35832042088815 25.09805827348358 68-69 25.266207754103263 25.20005912009063 24.228265936519268 25.30546718928684 70-71 24.179347274571857 25.651365237654467 24.49998942371567 25.66929806405801 72-73 25.154895708465126 25.498009114882812 24.357236360676467 24.989858815975598 74-75 25.51408105075251 25.150524623865827 24.345917058904014 24.98947726647765 76-77 24.777462928784658 25.431331666653723 25.25715097391513 24.534054430646485 78-79 25.143529549736783 25.477023892495687 24.941154357255105 24.438292200512425 80-81 24.341927523802664 25.426786541932557 25.322543202782715 24.908742731482064 82-83 24.387164567787423 25.708055460428497 24.2779677790151 25.626812192768984 84-85 24.617051487226394 25.8424545976179 23.994275686514648 25.54621822864106 86-87 24.904117280550803 24.924734341141665 24.33333931317342 25.837809065134117 88-89 24.879952479014108 24.614849210299642 24.95301586094569 25.552182449740556 90-91 25.39308969679801 24.9692685307881 24.56240288895892 25.07523888345497 92-93 24.471721291613328 25.251467894550967 24.680984456610595 25.595826357225114 94-95 23.869830305526097 25.479875472954035 25.15711137309356 25.493182848426315 96-97 25.143168081791362 25.136326966231664 24.45603090524158 25.264474046735398 98-99 25.19462371320758 25.17000372981369 24.172131303365052 25.463241253613678 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1554.0 1 1418.5 2 1308.0 3 1289.5 4 1171.0 5 1013.0 6 905.5 7 865.5 8 839.5 9 808.5 10 743.0 11 726.5 12 752.0 13 774.0 14 838.5 15 977.5 16 1132.5 17 1328.0 18 1659.0 19 2154.5 20 2923.5 21 4005.5 22 5583.0 23 7707.5 24 10391.5 25 14185.5 26 18917.5 27 23553.5 28 29261.0 29 36409.0 30 43244.0 31 50954.5 32 59470.0 33 68172.0 34 76383.0 35 85650.5 36 94465.0 37 103785.5 38 114995.5 39 120499.0 40 127887.5 41 138381.0 42 150986.5 43 173841.5 44 197525.5 45 219897.5 46 255724.0 47 294166.5 48 331506.0 49 411157.0 50 495925.0 51 441070.5 52 347777.0 53 330938.0 54 316162.5 55 303259.0 56 282573.0 57 265061.0 58 251323.0 59 223079.5 60 189566.0 61 150823.0 62 123959.0 63 100677.0 64 75657.5 65 58623.5 66 43478.5 67 32976.5 68 25816.0 69 21642.0 70 19103.5 71 16236.0 72 15068.0 73 13133.0 74 9494.5 75 7038.0 76 5162.0 77 3564.0 78 2286.5 79 1488.5 80 1009.0 81 687.0 82 467.0 83 344.0 84 254.5 85 188.0 86 142.5 87 95.0 88 62.5 89 51.5 90 36.5 91 27.5 92 21.0 93 19.0 94 19.5 95 15.5 96 7.0 97 4.5 98 4.5 99 8.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0021286445674982483 2 0.0 3 1.4726471850616811E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.6146794542423085E-4 22-23 0.0 24-25 0.004484880063596939 26-27 3.34692542059473E-5 28-29 0.008735475347752245 30-31 5.890588740246725E-4 32-33 6.0244657570705137E-5 34-35 6.69385084118946E-5 36-37 0.0 38-39 4.6856955888326216E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 2.0750937607687324E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.355080672951568E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7469542.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.65279951623732 #Duplication Level Percentage of deduplicated Percentage of total 1 74.90830142308128 31.201404612774798 2 13.507852413105597 11.2527973691602 3 4.633256912830803 5.789643638920864 4 2.127941182093449 3.5453882976033397 5 1.1412673339047128 2.376848972678184 6 0.7248534938049936 1.8115306353662146 7 0.520812469496086 1.5185308164233855 8 0.3476929613540048 1.158590816998817 9 0.2696155074634212 1.0107216610958225 >10 1.544033069441688 11.807109841518788 >50 0.1387100488523408 4.027604268221026 >100 0.11326186199490956 9.511333430792194 >500 0.014085545177336125 4.034252510436325 >1k 0.007509985016494925 5.801653842538735 >5k 4.512437345295557E-4 1.2059771396512453 >10k+ 3.5454864855893664E-4 3.9466121458199894 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 71574 0.9582113602145889 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 63182 0.8458617676960649 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 29704 0.3976682907733834 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 21464 0.28735362891058114 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 19502 0.2610869582097537 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 18939 0.25354968216257434 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 18406 0.2464140371658664 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 16793 0.22481967435218922 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 12399 0.16599411315981624 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 11355 0.15201735260341262 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 10648 0.14255224751397072 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 8152 0.10913654411475295 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 7710 0.10321917997114147 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 7699 0.10307191525263532 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 7542 0.10097004608850182 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3387701682378919E-5 2 0.0 1.3387701682378919E-5 0.0 0.0 1.3387701682378919E-5 3 0.0 1.3387701682378919E-5 0.0 0.0 1.3387701682378919E-5 4 0.0 1.3387701682378919E-5 0.0 1.3387701682378919E-5 1.3387701682378919E-5 5 0.0 1.3387701682378919E-5 0.0 2.6775403364757838E-5 2.6775403364757838E-5 6 1.3387701682378919E-5 1.3387701682378919E-5 0.0 2.6775403364757838E-5 4.016310504713676E-5 7 1.3387701682378919E-5 1.3387701682378919E-5 0.0 2.6775403364757838E-5 4.016310504713676E-5 8 1.3387701682378919E-5 1.3387701682378919E-5 0.0 2.6775403364757838E-5 4.016310504713676E-5 9 1.3387701682378919E-5 1.3387701682378919E-5 0.0 2.6775403364757838E-5 4.016310504713676E-5 10-11 1.3387701682378919E-5 1.3387701682378919E-5 0.0 4.6856955888326216E-5 4.016310504713676E-5 12-13 2.6775403364757838E-5 1.3387701682378919E-5 0.0 9.371391177665243E-5 4.016310504713676E-5 14-15 2.6775403364757838E-5 2.0081552523568378E-5 0.0 9.371391177665243E-5 4.6856955888326216E-5 16-17 2.6775403364757838E-5 2.6775403364757838E-5 0.0 1.4057086766497865E-4 9.371391177665243E-5 18-19 2.6775403364757838E-5 2.6775403364757838E-5 0.0 1.9412167439449433E-4 9.371391177665243E-5 20-21 4.6856955888326216E-5 2.6775403364757838E-5 0.0 3.748556471066097E-4 9.371391177665243E-5 22-23 6.69385084118946E-5 4.016310504713676E-5 0.0 0.0015195041409500074 9.371391177665243E-5 24-25 8.032621009427352E-5 4.6856955888326216E-5 0.0 0.004002922803031297 9.371391177665243E-5 26-27 1.2048931514141027E-4 5.3550806729515676E-5 0.0 0.006673769288665891 9.371391177665243E-5 28-29 1.338770168237892E-4 5.3550806729515676E-5 0.0 0.017671766220740175 9.371391177665243E-5 30-31 1.4726471850616811E-4 6.69385084118946E-5 0.0 0.05167652849398263 1.004077626178419E-4 32-33 1.8073397271211542E-4 6.69385084118946E-5 0.0 0.11251693878955363 1.0710161345903135E-4 34-35 1.8742782355330486E-4 7.363235925308406E-5 0.0 0.194074817438606 1.338770168237892E-4 36-37 1.8742782355330486E-4 8.702006093546298E-5 0.0 0.3131851993067313 1.6065242018854705E-4 38-39 1.8742782355330486E-4 9.371391177665243E-5 0.0 0.492058281484996 1.673462710297365E-4 40-41 1.8742782355330486E-4 1.2048931514141027E-4 0.0 0.7243750687793173 1.8073397271211542E-4 42-43 2.008155252356838E-4 1.2048931514141027E-4 0.0 0.9172583807681917 1.9412167439449433E-4 44-45 2.142032269180627E-4 1.2718316598259974E-4 0.0 1.1401502260781182 2.008155252356838E-4 46-47 2.142032269180627E-4 1.338770168237892E-4 0.0 1.3914173051038472 2.008155252356838E-4 48-49 2.142032269180627E-4 1.338770168237892E-4 0.0 1.6421020191063924 2.008155252356838E-4 50-51 2.142032269180627E-4 1.7404012187092596E-4 0.0 1.9145819114478506 2.008155252356838E-4 52-53 2.142032269180627E-4 1.8742782355330486E-4 0.0 2.202410803768156 2.1420322691806273E-4 54-55 2.142032269180627E-4 1.9412167439449433E-4 0.0 2.4821802996756697 2.2759092860044164E-4 56-57 2.142032269180627E-4 2.008155252356838E-4 0.0 2.795064275694547 2.2759092860044164E-4 58-59 2.142032269180627E-4 2.008155252356838E-4 0.0 3.1408016716419827 2.342847794416311E-4 60-61 2.142032269180627E-4 2.142032269180627E-4 0.0 3.4790955054540156 2.4767248112401E-4 62-63 2.2089707775925217E-4 2.2759092860044164E-4 0.0 3.822811358447412 2.543663319651995E-4 64-65 2.2759092860044164E-4 2.543663319651995E-4 0.0 4.189366630510946 2.543663319651995E-4 66-67 2.2759092860044164E-4 2.543663319651995E-4 0.0 4.589324218272017 2.543663319651995E-4 68-69 2.2759092860044164E-4 2.61060182806389E-4 0.0 5.031145149193886 2.543663319651995E-4 70-71 2.2759092860044164E-4 2.677540336475784E-4 0.0 5.555059466832104 2.543663319651995E-4 72-73 2.2759092860044164E-4 2.677540336475784E-4 0.0 6.035624406422777 2.811417353299573E-4 74-75 2.342847794416311E-4 2.677540336475784E-4 0.0 6.508625294562906 2.811417353299573E-4 76-77 2.4097863028282055E-4 2.811417353299573E-4 0.0 7.008388198366111 2.811417353299573E-4 78-79 2.4097863028282055E-4 2.811417353299573E-4 0.0 7.5224156983118915 2.811417353299573E-4 80-81 2.4097863028282055E-4 2.8783558617114673E-4 0.0 8.07987959636615 2.8783558617114673E-4 82-83 2.4097863028282055E-4 2.9452943701233623E-4 0.0 8.685003712409676 2.9452943701233623E-4 84-85 2.4097863028282055E-4 3.012232878535257E-4 0.0 9.265414131147534 2.9452943701233623E-4 86-87 2.4097863028282055E-4 3.34692542059473E-4 0.0 9.857947113758783 3.012232878535257E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 1915 0.0 22.582811 1 TCTAACG 815 0.0 21.109966 2 GTACAAG 3535 0.0 21.047201 1 GTACATA 1975 0.0 20.248608 1 CTAACGC 855 0.0 20.122368 3 GTATAAG 2585 0.0 19.427954 1 TAGTACT 2365 0.0 16.712107 4 ACGCCTA 1045 0.0 16.463755 6 GTATAAT 2100 0.0 16.16469 1 TAGGCAT 2795 0.0 16.137392 5 CATGGGG 17440 0.0 16.024017 4 GTATTAG 2560 0.0 15.80313 1 TACTAGG 2680 0.0 15.61533 2 TATATAC 2330 0.0 15.366616 3 GTATTAT 1760 0.0 15.324247 1 ACGTTAA 585 0.0 15.102234 8 GTATTAA 2145 0.0 14.958417 1 CTAGTAC 2320 0.0 14.831572 3 TATACAG 3310 0.0 14.750432 5 ATAAGGT 2040 0.0 14.587916 3 >>END_MODULE