FastQCFastQC Report
Fri 27 May 2016
ERR765422_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765422_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7469542
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA697560.9338725185560239No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT690120.9239120685043339No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG293990.3935850417602579No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC211010.2824938931998776No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC209040.27985651596844896No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198880.26625461105915194No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT193540.2591055783607616No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG177970.23826092684129763No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG174910.2341642901264897No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC136340.1825279247375542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125760.1683637363575973No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG121640.16284800326445717No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT104070.1393258114085174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101560.1359654982862403No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC84530.113166242321149No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG82500.1104485388796261No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA81410.1089892793962468No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG7400.027.0212
TAACGCA6050.021.5212424
AGTACTT288700.021.3271112-13
GTACTAG16100.019.9388521
TAGACCG5250.019.4862385
GAGTACT279150.018.7498912-13
TACTTTT331850.018.60302414-15
TAGTACT22800.018.5597114
GTATAAG23900.018.49281
TCTAACG8350.018.3777432
ATCACGC10800.017.6532553
TCACGCA11900.017.1937434
CTAACGC8150.017.1170253
CGTTAAC7100.017.0369551
GTACTTT314750.016.62178414-15
CATGGGG147450.016.214
CTAGTAC24100.016.2079073
ATAGGAC40950.015.7843083
GTATATA21100.015.6549921
ATGGGTA35750.015.478745
Produced by FastQC (version 0.11.5)