FastQCFastQC Report
Fri 27 May 2016
ERR765421_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765421_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7089925
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA91840.12953592597947086No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT85350.12038209148897909No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA240250.026.109491
CAACGCA270100.020.8654545
ATCAACG274700.020.651473
AACGCAG277350.020.5212156
TATCAAC303050.020.4840972
GTACATG384950.020.0638891
TCAACGC290650.019.4541874
TACATGG381050.019.30522
ACGCAGA292950.018.8887527
CGCAGAG295550.018.5652528
GTACTAG21800.018.3439041
CATGGGG174450.017.7788934
ACATGGG386850.017.7055723
GCAGAGT300950.017.5986449
GTACATA19850.017.5691431
GTACAAA44450.017.3654541
GTACAAG45350.016.8157541
TAGGCAT32800.016.3032365
GTATAAA102250.016.1896151
GTATATA20200.015.6533521
Produced by FastQC (version 0.11.5)